AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48275

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48275.t1 MTR_4g104020 98.848 521 6 0 1 521 1 521 0.0 1076
MS.gene48275.t1 MTR_8g442410 46.214 383 195 5 143 520 149 525 1.40e-104 323
MS.gene48275.t1 MTR_7g027190 41.191 403 207 7 133 521 288 674 6.53e-95 303
MS.gene48275.t1 MTR_3g065980 34.085 399 218 11 143 520 166 540 3.38e-66 224
MS.gene48275.t1 MTR_2g082090 32.376 383 237 5 146 519 209 578 6.32e-63 216
MS.gene48275.t1 MTR_3g089055 32.808 381 236 5 148 520 202 570 1.22e-61 212
MS.gene48275.t1 MTR_3g089055 32.808 381 236 5 148 520 197 565 1.50e-61 212
MS.gene48275.t1 MTR_1g029420 32.461 382 236 5 148 520 224 592 2.84e-61 211
MS.gene48275.t1 MTR_5g097480 32.292 384 238 6 146 520 174 544 3.36e-60 207
MS.gene48275.t1 MTR_4g064200 32.642 386 231 9 146 519 281 649 6.67e-58 204
MS.gene48275.t1 MTR_5g094450 30.928 388 242 7 146 520 158 532 8.00e-58 201
MS.gene48275.t1 MTR_4g102790 34.109 387 234 12 143 519 286 661 2.66e-56 200
MS.gene48275.t1 MTR_4g064160 29.688 384 247 6 146 519 313 683 1.19e-55 198
MS.gene48275.t1 MTR_3g056110 29.948 384 247 5 146 520 172 542 1.31e-55 195
MS.gene48275.t1 MTR_4g064120 29.306 389 244 8 146 518 251 624 1.68e-52 188
MS.gene48275.t1 MTR_2g097467 29.870 385 242 7 146 519 286 653 2.67e-52 188
MS.gene48275.t1 MTR_4g064150 28.713 404 257 8 132 519 344 732 8.58e-52 188
MS.gene48275.t1 MTR_4g133660 30.670 388 246 7 144 521 170 544 2.17e-51 184
MS.gene48275.t1 MTR_4g064180 28.792 389 246 8 146 518 251 624 3.09e-51 185
MS.gene48275.t1 MTR_7g062120 29.870 385 247 6 146 520 369 740 3.20e-51 186
MS.gene48275.t1 MTR_2g097410 29.950 404 246 10 134 519 356 740 6.06e-51 186
MS.gene48275.t1 MTR_2g026250 31.458 391 239 9 144 521 223 597 9.40e-51 183
MS.gene48275.t1 MTR_7g074650 31.622 370 232 8 153 520 442 792 3.39e-50 184
MS.gene48275.t1 MTR_4g076140 28.118 441 241 10 143 520 42 469 4.68e-49 176
MS.gene48275.t1 MTR_2g097473 27.594 424 279 7 107 519 247 653 9.60e-49 178
MS.gene48275.t1 MTR_2g097463 28.831 385 246 7 146 519 287 654 4.26e-48 177
MS.gene48275.t1 MTR_2g097390 28.010 382 254 8 146 519 272 640 1.53e-46 172
MS.gene48275.t1 MTR_5g009080 28.182 440 245 13 143 521 50 479 1.12e-45 167
MS.gene48275.t1 MTR_2g097310 28.721 383 250 10 146 519 269 637 1.17e-45 169
MS.gene48275.t1 MTR_2g097350 27.487 382 256 8 146 519 271 639 1.22e-45 169
MS.gene48275.t1 MTR_1g096030 30.097 412 232 15 141 520 72 459 8.47e-45 164
MS.gene48275.t1 MTR_2g034250 29.136 405 265 9 127 520 192 585 1.19e-44 166
MS.gene48275.t1 MTR_3g072710 30.732 410 251 11 133 521 104 501 4.31e-44 163
MS.gene48275.t1 MTR_4g076020 31.720 372 220 11 153 520 88 429 9.16e-44 160
MS.gene48275.t1 MTR_2g034260 29.177 401 255 12 133 520 200 584 8.39e-43 161
MS.gene48275.t1 MTR_1g069725 28.728 456 273 15 86 521 41 464 8.59e-43 159
MS.gene48275.t1 MTR_4g077760 27.532 385 250 7 146 520 190 555 3.92e-42 158
MS.gene48275.t1 MTR_5g058860 29.440 411 259 10 130 521 100 498 6.85e-41 154
MS.gene48275.t1 MTR_3g022830 26.241 423 248 6 150 520 26 436 1.03e-40 152
MS.gene48275.t1 MTR_1g086970 30.179 391 246 13 144 520 101 478 2.92e-40 152
MS.gene48275.t1 MTR_2g034280 28.321 399 259 11 133 520 193 575 5.40e-40 153
MS.gene48275.t1 MTR_2g097380 25.737 373 229 8 154 519 229 560 4.95e-39 150
MS.gene48275.t1 MTR_7g109580 26.500 400 272 11 127 520 171 554 3.50e-36 142
MS.gene48275.t1 MTR_4g097080 26.221 389 252 10 149 520 133 503 4.35e-36 140
MS.gene48275.t1 MTR_7g057230 30.592 304 199 6 224 519 357 656 8.38e-35 139
MS.gene48275.t1 MTR_5g015490 25.128 390 261 10 149 520 114 490 4.62e-33 132
MS.gene48275.t1 MTR_2g089100 25.829 422 260 11 132 519 40 442 3.14e-31 126
MS.gene48275.t1 MTR_6g047750 29.847 392 233 15 148 520 294 662 2.16e-30 125
MS.gene48275.t1 MTR_5g015950 25.891 421 254 13 148 519 34 445 1.78e-26 112
MS.gene48275.t1 MTR_3g053270 25.891 421 254 13 148 519 34 445 1.78e-26 112
MS.gene48275.t1 MTR_2g099110 24.824 427 267 14 121 520 52 451 4.87e-26 111
MS.gene48275.t1 MTR_4g095500 26.047 430 240 14 148 519 58 467 1.23e-25 110
MS.gene48275.t1 MTR_0092s0100 25.389 386 253 11 146 521 372 732 1.91e-22 101
MS.gene48275.t1 MTR_0092s0100 25.389 386 253 11 146 521 369 729 2.52e-22 101
MS.gene48275.t1 MTR_8g093070 27.366 391 236 16 146 519 136 495 2.18e-21 97.8
MS.gene48275.t1 MTR_4g026485 24.685 397 261 12 136 521 256 625 1.31e-20 95.9
MS.gene48275.t1 MTR_8g020840 24.146 410 255 13 142 520 164 548 7.91e-20 93.2
MS.gene48275.t1 MTR_3g022580 34.109 129 80 2 397 520 55 183 1.06e-17 81.6
MS.gene48275.t1 MTR_3g022005 34.653 101 66 0 420 520 25 125 1.02e-15 74.3
MS.gene48275.t1 MTR_8g077940 25.000 304 198 11 231 521 256 542 2.76e-15 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48275.t1 AT1G66350 36.554 383 215 10 143 521 149 507 1.25e-73 242
MS.gene48275.t1 AT1G14920 36.132 393 215 11 139 520 162 529 1.40e-69 232
MS.gene48275.t1 AT3G03450 35.294 391 218 12 143 520 177 545 2.70e-69 232
MS.gene48275.t1 AT5G17490 35.990 389 212 13 143 520 154 516 7.59e-69 230
MS.gene48275.t1 AT2G01570 34.359 390 220 11 142 520 217 581 4.31e-66 224
MS.gene48275.t1 AT5G48150 33.684 380 231 6 150 520 123 490 1.40e-63 215
MS.gene48275.t1 AT5G48150 33.684 380 231 6 150 520 123 490 1.40e-63 215
MS.gene48275.t1 AT5G48150 33.684 380 231 6 150 520 123 490 1.40e-63 215
MS.gene48275.t1 AT5G48150 33.684 380 231 6 150 520 123 490 1.40e-63 215
MS.gene48275.t1 AT1G21450 32.376 383 236 6 148 520 224 593 7.45e-60 208
MS.gene48275.t1 AT2G04890 31.733 375 239 5 149 520 53 413 6.12e-59 201
MS.gene48275.t1 AT1G50420 31.066 441 231 10 143 521 51 480 6.31e-58 200
MS.gene48275.t1 AT1G50600 32.031 384 239 6 146 520 156 526 5.36e-56 196
MS.gene48275.t1 AT1G50600 32.031 384 239 6 146 520 227 597 1.23e-55 196
MS.gene48275.t1 AT1G50600 32.031 384 239 6 146 520 231 601 1.80e-55 196
MS.gene48275.t1 AT2G29065 31.620 389 235 10 149 519 252 627 2.41e-55 196
MS.gene48275.t1 AT3G54220 34.054 370 224 9 153 519 297 649 3.62e-54 193
MS.gene48275.t1 AT3G46600 30.729 384 238 8 149 520 4 371 2.97e-53 185
MS.gene48275.t1 AT1G07520 31.105 389 236 10 149 519 154 528 5.11e-53 188
MS.gene48275.t1 AT1G07520 31.105 389 236 10 149 519 154 528 5.11e-53 188
MS.gene48275.t1 AT5G59450 30.670 388 243 8 146 520 224 598 2.40e-52 187
MS.gene48275.t1 AT1G07520 31.105 389 236 10 149 519 318 692 2.80e-52 189
MS.gene48275.t1 AT1G07520 31.105 389 236 10 149 519 319 693 2.95e-52 189
MS.gene48275.t1 AT3G46600 30.729 384 238 8 149 520 212 579 1.39e-51 185
MS.gene48275.t1 AT3G46600 30.729 384 238 8 149 520 180 547 1.54e-51 184
MS.gene48275.t1 AT5G41920 31.514 403 254 10 122 521 18 401 2.36e-51 181
MS.gene48275.t1 AT4G17230 31.606 386 242 6 144 520 153 525 1.10e-50 182
MS.gene48275.t1 AT4G17230 31.606 386 242 6 144 520 153 525 1.10e-50 182
MS.gene48275.t1 AT1G07530 28.385 384 253 5 146 519 393 764 1.16e-50 185
MS.gene48275.t1 AT2G37650 29.199 387 245 8 146 520 332 701 8.70e-49 179
MS.gene48275.t1 AT2G37650 29.199 387 245 8 146 520 344 713 9.05e-49 179
MS.gene48275.t1 AT2G29060 29.208 404 252 10 133 519 268 654 2.37e-48 177
MS.gene48275.t1 AT2G29060 29.208 404 252 10 133 519 305 691 3.45e-48 177
MS.gene48275.t1 AT1G63100 29.847 392 245 8 146 519 249 628 4.36e-45 168
MS.gene48275.t1 AT1G63100 29.847 392 245 8 146 519 274 653 5.27e-45 168
MS.gene48275.t1 AT1G55580 34.680 297 176 6 238 520 153 445 8.35e-45 164
MS.gene48275.t1 AT5G66770 27.792 385 251 7 146 520 217 584 1.11e-43 163
MS.gene48275.t1 AT3G50650 29.412 357 216 7 180 520 206 542 2.20e-41 156
MS.gene48275.t1 AT5G52510 30.128 312 190 8 232 520 334 640 2.05e-35 140
MS.gene48275.t1 AT4G08250 28.244 393 249 12 143 519 101 476 1.26e-34 136
MS.gene48275.t1 AT4G37650 26.551 403 246 14 149 520 146 529 4.70e-32 129
MS.gene48275.t1 AT3G49950 26.173 405 256 9 140 519 21 407 2.74e-28 117
MS.gene48275.t1 AT2G45160 26.111 360 216 14 181 521 312 640 1.82e-24 107
MS.gene48275.t1 AT3G60630 27.796 313 187 13 230 521 329 623 4.34e-24 106
MS.gene48275.t1 AT4G00150 25.140 358 222 12 188 521 220 555 6.78e-22 99.8
MS.gene48275.t1 AT4G36710 25.604 414 255 17 126 520 106 485 6.31e-19 90.1
MS.gene48275.t1 AT3G13840 24.818 411 253 15 133 520 133 510 9.73e-14 73.9

Find 96 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAATTGTTGAGAAGAATT+TGG 0.186388 8.1:+20410843 MS.gene48275:CDS
AATTGTCCACATATTCAATT+TGG 0.204871 8.1:+20410565 MS.gene48275:CDS
CAACCGTAACTATCCCTATT+TGG 0.207024 8.1:+20410050 MS.gene48275:CDS
CTGAAATTCGCAAACCAAAT+AGG 0.213265 8.1:-20410064 None:intergenic
CTCAAAGGCTTCCAATATTA+TGG 0.226205 8.1:-20410605 None:intergenic
TGTGGCTAATTCCATAATAT+TGG 0.269201 8.1:+20410594 MS.gene48275:CDS
GAGAAAGGGTGTTTGGTTCT+TGG 0.272544 8.1:+20411354 MS.gene48275:CDS
CATGATCAGAAAATGGATTA+AGG 0.277342 8.1:-20409988 None:intergenic
AAATCAAATGCTTTGGTGTT+TGG 0.284256 8.1:+20410373 MS.gene48275:CDS
GCTTGAACAACCATCTTAAT+AGG 0.288790 8.1:-20411279 None:intergenic
TCAGTTTCATGATCAGAAAA+TGG 0.293107 8.1:-20409995 None:intergenic
TTCTGCTATATTTGACTCAT+TGG 0.296218 8.1:+20411089 MS.gene48275:CDS
TATTGAAGAAACTATTACTA+TGG 0.299092 8.1:-20410090 None:intergenic
GTTATGGCTGAGCAAGATTC+TGG 0.301485 8.1:+20411015 MS.gene48275:CDS
CAATTTCTTGTGATCCCTAA+TGG 0.315162 8.1:-20410020 None:intergenic
AGATGAAAATCCATCATTAA+TGG 0.342726 8.1:+20409898 MS.gene48275:CDS
TTCATCTACCTCACATTTGA+AGG 0.343224 8.1:-20409882 None:intergenic
TTTCTTCACGTGGTTGATCT+TGG 0.361819 8.1:+20410637 MS.gene48275:CDS
CAGCAAGAAACTGCAACAAT+AGG 0.367426 8.1:-20411390 None:intergenic
CTATTGTTGCAGTTTCTTGC+TGG 0.367470 8.1:+20411391 MS.gene48275:CDS
CAAAACAAGAAGCAACTCTT+TGG 0.370059 8.1:-20410405 None:intergenic
ATCATGAAACTGAAACCATT+AGG 0.377820 8.1:+20410005 MS.gene48275:CDS
CATAATATTGGAAGCCTTTG+AGG 0.384786 8.1:+20410606 MS.gene48275:CDS
TGAAATTCGCAAACCAAATA+GGG 0.387079 8.1:-20410063 None:intergenic
CGAGCTTAAATCAAATGCTT+TGG 0.395337 8.1:+20410366 MS.gene48275:CDS
GATGAAAATCCATCATTAAT+GGG 0.395439 8.1:+20409899 MS.gene48275:CDS
GGATGCTGCATCAGAAGAAA+TGG 0.398391 8.1:+20410516 MS.gene48275:CDS
CCAGCAGAATTATTACCAAT+TGG 0.406382 8.1:-20410463 None:intergenic
AAAATCAATGATGAACATAA+TGG 0.412525 8.1:+20410495 MS.gene48275:CDS
TTCGCCGAAAGTGTTGGTTA+TGG 0.421237 8.1:+20410999 MS.gene48275:CDS
TCATGAAACTGAAACCATTA+GGG 0.431360 8.1:+20410006 MS.gene48275:CDS
AGGAGGAGAATGAGTAGGGC+TGG 0.435845 8.1:+20411243 MS.gene48275:CDS
ATGAGTAGGGCTGGATTTCA+AGG 0.440915 8.1:+20411252 MS.gene48275:CDS
GTTGATCTTGGCATGAGCCT+TGG 0.453420 8.1:+20410649 MS.gene48275:CDS
AAGGGTGTTTGGTTCTTGGA+TGG 0.458218 8.1:+20411358 MS.gene48275:CDS
TTTGGAGAATTTGAAACCTA+AGG 0.459147 8.1:+20410861 MS.gene48275:CDS
TCATGCCTCTCCATCCTCAA+TGG 0.459152 8.1:-20411204 None:intergenic
TCAAGGTTCACCTATTAAGA+TGG 0.464234 8.1:+20411269 MS.gene48275:CDS
CTAACTCTGATTCAACCAAT+TGG 0.465189 8.1:+20410448 MS.gene48275:CDS
GCCACCATGTACAGCGGCTT+AGG 0.465205 8.1:+20410728 MS.gene48275:CDS
CCAATTGGTAATAATTCTGC+TGG 0.468263 8.1:+20410463 MS.gene48275:CDS
TGTGGAAGAGAAAGGGTGTT+TGG 0.469216 8.1:+20411347 MS.gene48275:CDS
GCCCTCTTAGTGTCATACTT+TGG 0.484118 8.1:-20411123 None:intergenic
AAATGTCCAAATTGAATATG+TGG 0.495894 8.1:-20410571 None:intergenic
TGCCAAAGTATGACACTAAG+AGG 0.499635 8.1:+20411121 MS.gene48275:CDS
TATACTGTTGTGGAAGAGAA+AGG 0.500286 8.1:+20411339 MS.gene48275:CDS
AGAGTTCTTCACCAATGACT+TGG 0.501290 8.1:-20410783 None:intergenic
AAGCCGCTGTACATGGTGGC+TGG 0.503998 8.1:-20410725 None:intergenic
TCGCGACAAATCACACGCTT+CGG 0.504012 8.1:+20410333 MS.gene48275:CDS
ATAATATTGGAAGCCTTTGA+GGG 0.506937 8.1:+20410607 MS.gene48275:CDS
AAGAACATTGTGAGCTGTGA+AGG 0.508905 8.1:+20411180 MS.gene48275:CDS
ATTGCTAGAATCCAAGTCAT+TGG 0.519171 8.1:+20410772 MS.gene48275:CDS
TTTGAATTAGTCCTCGCCAT+TGG 0.520307 8.1:-20410687 None:intergenic
TGATCTCTGAAATGAAGCCT+TGG 0.520426 8.1:-20410148 None:intergenic
TCAGCCATAACCAACACTTT+CGG 0.520632 8.1:-20411003 None:intergenic
CAAAGCATTTGATTTAAGCT+CGG 0.521032 8.1:-20410365 None:intergenic
TAGTAAGAACCCATTAATGA+TGG 0.522254 8.1:-20409908 None:intergenic
CGGTCTTTCGCCGAAAGTGT+TGG 0.527317 8.1:+20410993 MS.gene48275:CDS
AGAAAGTTGATCAGTGGAGG+AGG 0.527630 8.1:+20411226 MS.gene48275:CDS
TGATCCTAAGCCGCTGTACA+TGG 0.536386 8.1:-20410732 None:intergenic
ATACTGTTGTGGAAGAGAAA+GGG 0.539638 8.1:+20411340 MS.gene48275:CDS
AAGAAAACTCTCACCCTCAA+AGG 0.545556 8.1:-20410620 None:intergenic
AATGGAAGAAGCATTCAAGT+TGG 0.548963 8.1:+20410534 MS.gene48275:CDS
ATGATTCCATAAACCTTCCA+AGG 0.552621 8.1:-20411056 None:intergenic
AGTGGAGGAGGAGAATGAGT+AGG 0.556868 8.1:+20411238 MS.gene48275:CDS
GTGAAGAATAATGTTTGTGA+TGG 0.562851 8.1:+20411315 MS.gene48275:CDS
GTGGAGGAGGAGAATGAGTA+GGG 0.563946 8.1:+20411239 MS.gene48275:CDS
GGCATGAGAAAGTTGATCAG+TGG 0.565335 8.1:+20411220 MS.gene48275:CDS
AATTGCGAAGCTGAAGAAGA+TGG 0.566408 8.1:+20410247 MS.gene48275:CDS
TGCTAGTACCTTCAAATGTG+AGG 0.569925 8.1:+20409874 MS.gene48275:CDS
CGGCTTAGGATCACAGCGAT+TGG 0.575772 8.1:+20410742 MS.gene48275:CDS
GTCTTCCACATGGACACCAA+TGG 0.579517 8.1:+20410671 MS.gene48275:CDS
AAACCAAATAGGGATAGTTA+CGG 0.582805 8.1:-20410053 None:intergenic
GAAGATGGTTCATGTGCTGA+TGG 0.596375 8.1:+20410262 MS.gene48275:CDS
AAGGTACTAGCATAAAGTGC+AGG 0.596660 8.1:-20409863 None:intergenic
ATGAGAAAGTTGATCAGTGG+AGG 0.606341 8.1:+20411223 MS.gene48275:CDS
CTGTGAAGGACCATTGAGGA+TGG 0.613485 8.1:+20411194 MS.gene48275:CDS
GAGTCGTGGCGCTTTGAATG+CGG 0.617247 8.1:+20410954 MS.gene48275:CDS
AAGGACCATTGAGGATGGAG+AGG 0.621814 8.1:+20411199 MS.gene48275:CDS
ATGAGCCTTGGTCTTCCACA+TGG 0.634567 8.1:+20410661 MS.gene48275:CDS
AAAACAGTGCTAGTTCAACA+TGG 0.638515 8.1:+20409963 MS.gene48275:CDS
GAGCAAGATTCTGGTCACAA+TGG 0.639301 8.1:+20411024 MS.gene48275:CDS
TTGTGATGGATATACTGTTG+TGG 0.639465 8.1:+20411329 MS.gene48275:CDS
TGGTTGTTCAAGCTAAGCAG+TGG 0.641582 8.1:+20411289 MS.gene48275:CDS
ACAGTGTGAATAGCATTCCA+AGG 0.646338 8.1:+20410131 MS.gene48275:CDS
GGTGTCCATGTGGAAGACCA+AGG 0.647949 8.1:-20410666 None:intergenic
ACACTTTCGGCGAAAGACCG+TGG 0.648958 8.1:-20410990 None:intergenic
CCTCGCCATTGGTGTCCATG+TGG 0.649204 8.1:-20410676 None:intergenic
GCCAAAGTATGACACTAAGA+GGG 0.650623 8.1:+20411122 MS.gene48275:CDS
TGTGTTGTTAAAGAGAGTCG+TGG 0.651261 8.1:+20410940 MS.gene48275:CDS
CCACATGGACACCAATGGCG+AGG 0.669979 8.1:+20410676 MS.gene48275:CDS
GAAGAATGCTGTTAACCACA+AGG 0.689238 8.1:-20410909 None:intergenic
GATCCAGCCACCATGTACAG+CGG 0.699851 8.1:+20410722 MS.gene48275:CDS
TCCTAAGCCGCTGTACATGG+TGG 0.702447 8.1:-20410729 None:intergenic
TGAGCTGTGAAGGACCATTG+AGG 0.705754 8.1:+20411190 MS.gene48275:CDS
GGTGGCTGGATCGATCTGCG+AGG 0.732660 8.1:-20410711 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATCAATGATGAACATAA+TGG + chr8.1:20410495-20410514 MS.gene48275:CDS 20.0%
!! TATTGAAGAAACTATTACTA+TGG - chr8.1:20410093-20410112 None:intergenic 20.0%
!!! TTTTCTAAAATAGGCAAATT+TGG - chr8.1:20409947-20409966 None:intergenic 20.0%
! AAATGTCCAAATTGAATATG+TGG - chr8.1:20410574-20410593 None:intergenic 25.0%
! AATTGTCCACATATTCAATT+TGG + chr8.1:20410565-20410584 MS.gene48275:CDS 25.0%
! AGATGAAAATCCATCATTAA+TGG + chr8.1:20409898-20409917 MS.gene48275:CDS 25.0%
! CATAAAAGTGAATGAAAAAG+AGG + chr8.1:20410885-20410904 MS.gene48275:CDS 25.0%
! GATGAAAATCCATCATTAAT+GGG + chr8.1:20409899-20409918 MS.gene48275:CDS 25.0%
! TCAATTTACGCGAAAAATTT+GGG + chr8.1:20410805-20410824 MS.gene48275:CDS 25.0%
!! TATTCAATTTGGACATTTTG+TGG + chr8.1:20410576-20410595 MS.gene48275:CDS 25.0%
AAACCAAATAGGGATAGTTA+CGG - chr8.1:20410056-20410075 None:intergenic 30.0%
ATAAACCTTCCAAGGAAAAA+AGG - chr8.1:20411051-20411070 None:intergenic 30.0%
ATAATATTGGAAGCCTTTGA+GGG + chr8.1:20410607-20410626 MS.gene48275:CDS 30.0%
ATCATGAAACTGAAACCATT+AGG + chr8.1:20410005-20410024 MS.gene48275:CDS 30.0%
CAATTTACGCGAAAAATTTG+GGG + chr8.1:20410806-20410825 MS.gene48275:CDS 30.0%
CTCAATTGTTGAGAAGAATT+TGG + chr8.1:20410843-20410862 MS.gene48275:CDS 30.0%
CTCAATTTACGCGAAAAATT+TGG + chr8.1:20410804-20410823 MS.gene48275:CDS 30.0%
GAAAAATTTGGGGATTCATT+TGG + chr8.1:20410816-20410835 MS.gene48275:CDS 30.0%
GTGAAGAATAATGTTTGTGA+TGG + chr8.1:20411315-20411334 MS.gene48275:CDS 30.0%
TAGTAAGAACCCATTAATGA+TGG - chr8.1:20409911-20409930 None:intergenic 30.0%
TCAGTTTCATGATCAGAAAA+TGG - chr8.1:20409998-20410017 None:intergenic 30.0%
TCATGAAACTGAAACCATTA+GGG + chr8.1:20410006-20410025 MS.gene48275:CDS 30.0%
TGAAATTCGCAAACCAAATA+GGG - chr8.1:20410066-20410085 None:intergenic 30.0%
TGTGGCTAATTCCATAATAT+TGG + chr8.1:20410594-20410613 MS.gene48275:CDS 30.0%
TTCTGCTATATTTGACTCAT+TGG + chr8.1:20411089-20411108 MS.gene48275:CDS 30.0%
! CAAAGCATTTGATTTAAGCT+CGG - chr8.1:20410368-20410387 None:intergenic 30.0%
! TTTGGAGAATTTGAAACCTA+AGG + chr8.1:20410861-20410880 MS.gene48275:CDS 30.0%
!! AAATCAAATGCTTTGGTGTT+TGG + chr8.1:20410373-20410392 MS.gene48275:CDS 30.0%
!! CATGATCAGAAAATGGATTA+AGG - chr8.1:20409991-20410010 None:intergenic 30.0%
!! TCATTCACTTTTATGTCCTT+AGG - chr8.1:20410880-20410899 None:intergenic 30.0%
!!! CTAGCACTGTTTTCTAAAAT+AGG - chr8.1:20409956-20409975 None:intergenic 30.0%
!!! TTTTTTCCTTGGAAGGTTTA+TGG + chr8.1:20411050-20411069 MS.gene48275:CDS 30.0%
AAAACAGTGCTAGTTCAACA+TGG + chr8.1:20409963-20409982 MS.gene48275:CDS 35.0%
ATACTGTTGTGGAAGAGAAA+GGG + chr8.1:20411340-20411359 MS.gene48275:CDS 35.0%
ATGATTCCATAAACCTTCCA+AGG - chr8.1:20411059-20411078 None:intergenic 35.0%
ATTGCTAGAATCCAAGTCAT+TGG + chr8.1:20410772-20410791 MS.gene48275:CDS 35.0%
CAAAACAAGAAGCAACTCTT+TGG - chr8.1:20410408-20410427 None:intergenic 35.0%
CAATTTCTTGTGATCCCTAA+TGG - chr8.1:20410023-20410042 None:intergenic 35.0%
CATAATATTGGAAGCCTTTG+AGG + chr8.1:20410606-20410625 MS.gene48275:CDS 35.0%
CCAGCAGAATTATTACCAAT+TGG - chr8.1:20410466-20410485 None:intergenic 35.0%
CGAGCTTAAATCAAATGCTT+TGG + chr8.1:20410366-20410385 MS.gene48275:CDS 35.0%
CTAACTCTGATTCAACCAAT+TGG + chr8.1:20410448-20410467 MS.gene48275:CDS 35.0%
CTCAAAGGCTTCCAATATTA+TGG - chr8.1:20410608-20410627 None:intergenic 35.0%
CTGAAATTCGCAAACCAAAT+AGG - chr8.1:20410067-20410086 None:intergenic 35.0%
GCTTGAACAACCATCTTAAT+AGG - chr8.1:20411282-20411301 None:intergenic 35.0%
TATACTGTTGTGGAAGAGAA+AGG + chr8.1:20411339-20411358 MS.gene48275:CDS 35.0%
TCAAGGTTCACCTATTAAGA+TGG + chr8.1:20411269-20411288 MS.gene48275:CDS 35.0%
TTCATCTACCTCACATTTGA+AGG - chr8.1:20409885-20409904 None:intergenic 35.0%
! CCAATTGGTAATAATTCTGC+TGG + chr8.1:20410463-20410482 MS.gene48275:CDS 35.0%
!! AATGGAAGAAGCATTCAAGT+TGG + chr8.1:20410534-20410553 MS.gene48275:CDS 35.0%
!! TTGTGATGGATATACTGTTG+TGG + chr8.1:20411329-20411348 MS.gene48275:CDS 35.0%
!!! GGAACAGTTTTATTTTGCTG+AGG + chr8.1:20411152-20411171 MS.gene48275:CDS 35.0%
!!! GTTGCTTCTTGTTTTGTTCA+AGG + chr8.1:20410412-20410431 MS.gene48275:CDS 35.0%
AAGAAAACTCTCACCCTCAA+AGG - chr8.1:20410623-20410642 None:intergenic 40.0%
AAGAACATTGTGAGCTGTGA+AGG + chr8.1:20411180-20411199 MS.gene48275:CDS 40.0%
AATTGCGAAGCTGAAGAAGA+TGG + chr8.1:20410247-20410266 MS.gene48275:CDS 40.0%
ACAGTGTGAATAGCATTCCA+AGG + chr8.1:20410131-20410150 MS.gene48275:CDS 40.0%
AGAGTTCTTCACCAATGACT+TGG - chr8.1:20410786-20410805 None:intergenic 40.0%
CAACCGTAACTATCCCTATT+TGG + chr8.1:20410050-20410069 MS.gene48275:CDS 40.0%
CAGCAAGAAACTGCAACAAT+AGG - chr8.1:20411393-20411412 None:intergenic 40.0%
CTATTGTTGCAGTTTCTTGC+TGG + chr8.1:20411391-20411410 MS.gene48275:CDS 40.0%
GAAGAATGCTGTTAACCACA+AGG - chr8.1:20410912-20410931 None:intergenic 40.0%
GAATGAAAAAGAGGTCCTTG+TGG + chr8.1:20410894-20410913 MS.gene48275:CDS 40.0%
GCCAAAGTATGACACTAAGA+GGG + chr8.1:20411122-20411141 MS.gene48275:CDS 40.0%
TCAGCCATAACCAACACTTT+CGG - chr8.1:20411006-20411025 None:intergenic 40.0%
TGACACTAAGAGGGCAAAAA+TGG + chr8.1:20411131-20411150 MS.gene48275:CDS 40.0%
TGCCAAAGTATGACACTAAG+AGG + chr8.1:20411121-20411140 MS.gene48275:CDS 40.0%
TGCTAGTACCTTCAAATGTG+AGG + chr8.1:20409874-20409893 MS.gene48275:CDS 40.0%
TTTCTTCACGTGGTTGATCT+TGG + chr8.1:20410637-20410656 MS.gene48275:CDS 40.0%
TTTGAATTAGTCCTCGCCAT+TGG - chr8.1:20410690-20410709 None:intergenic 40.0%
! AAGGTACTAGCATAAAGTGC+AGG - chr8.1:20409866-20409885 None:intergenic 40.0%
! ATGAGAAAGTTGATCAGTGG+AGG + chr8.1:20411223-20411242 MS.gene48275:CDS 40.0%
! GCGGTTTTACAAATGATCCA+CGG + chr8.1:20410973-20410992 MS.gene48275:CDS 40.0%
! TGTGTTGTTAAAGAGAGTCG+TGG + chr8.1:20410940-20410959 MS.gene48275:CDS 40.0%
!! TGATCTCTGAAATGAAGCCT+TGG - chr8.1:20410151-20410170 None:intergenic 40.0%
!!! ATGGACCTTTTTTCCTTGGA+AGG + chr8.1:20411043-20411062 MS.gene48275:CDS 40.0%
!!! CACAATGGACCTTTTTTCCT+TGG + chr8.1:20411039-20411058 MS.gene48275:CDS 40.0%
ATGAGTAGGGCTGGATTTCA+AGG + chr8.1:20411252-20411271 MS.gene48275:CDS 45.0%
GAAGATGGTTCATGTGCTGA+TGG + chr8.1:20410262-20410281 MS.gene48275:CDS 45.0%
GAGCAAGATTCTGGTCACAA+TGG + chr8.1:20411024-20411043 MS.gene48275:CDS 45.0%
GCCCTCTTAGTGTCATACTT+TGG - chr8.1:20411126-20411145 None:intergenic 45.0%
GGATGCTGCATCAGAAGAAA+TGG + chr8.1:20410516-20410535 MS.gene48275:CDS 45.0%
GTTATGGCTGAGCAAGATTC+TGG + chr8.1:20411015-20411034 MS.gene48275:CDS 45.0%
TGGTTGTTCAAGCTAAGCAG+TGG + chr8.1:20411289-20411308 MS.gene48275:CDS 45.0%
! AGAAAGTTGATCAGTGGAGG+AGG + chr8.1:20411226-20411245 MS.gene48275:CDS 45.0%
! GGCATGAGAAAGTTGATCAG+TGG + chr8.1:20411220-20411239 MS.gene48275:CDS 45.0%
!! AAGGGTGTTTGGTTCTTGGA+TGG + chr8.1:20411358-20411377 MS.gene48275:CDS 45.0%
!! GAGAAAGGGTGTTTGGTTCT+TGG + chr8.1:20411354-20411373 MS.gene48275:CDS 45.0%
!! TGTGGAAGAGAAAGGGTGTT+TGG + chr8.1:20411347-20411366 MS.gene48275:CDS 45.0%
!! TTCGCCGAAAGTGTTGGTTA+TGG + chr8.1:20410999-20411018 MS.gene48275:CDS 45.0%
AAGGACCATTGAGGATGGAG+AGG + chr8.1:20411199-20411218 MS.gene48275:CDS 50.0%
AGTGGAGGAGGAGAATGAGT+AGG + chr8.1:20411238-20411257 MS.gene48275:CDS 50.0%
CTGTGAAGGACCATTGAGGA+TGG + chr8.1:20411194-20411213 MS.gene48275:CDS 50.0%
GTCTTCCACATGGACACCAA+TGG + chr8.1:20410671-20410690 MS.gene48275:CDS 50.0%
GTGGAGGAGGAGAATGAGTA+GGG + chr8.1:20411239-20411258 MS.gene48275:CDS 50.0%
GTTGATCTTGGCATGAGCCT+TGG + chr8.1:20410649-20410668 MS.gene48275:CDS 50.0%
TCATGCCTCTCCATCCTCAA+TGG - chr8.1:20411207-20411226 None:intergenic 50.0%
TCGCGACAAATCACACGCTT+CGG + chr8.1:20410333-20410352 MS.gene48275:CDS 50.0%
TGAGCTGTGAAGGACCATTG+AGG + chr8.1:20411190-20411209 MS.gene48275:CDS 50.0%
TGATCCTAAGCCGCTGTACA+TGG - chr8.1:20410735-20410754 None:intergenic 50.0%
! ATGAGCCTTGGTCTTCCACA+TGG + chr8.1:20410661-20410680 MS.gene48275:CDS 50.0%
! GGGTGAGAGTTTTCTTCACG+TGG + chr8.1:20410627-20410646 MS.gene48275:CDS 50.0%
ACACTTTCGGCGAAAGACCG+TGG - chr8.1:20410993-20411012 None:intergenic 55.0%
AGGAGGAGAATGAGTAGGGC+TGG + chr8.1:20411243-20411262 MS.gene48275:CDS 55.0%
CGGCTTAGGATCACAGCGAT+TGG + chr8.1:20410742-20410761 MS.gene48275:CDS 55.0%
GATCCAGCCACCATGTACAG+CGG + chr8.1:20410722-20410741 MS.gene48275:CDS 55.0%
GGTGTCCATGTGGAAGACCA+AGG - chr8.1:20410669-20410688 None:intergenic 55.0%
TCCTAAGCCGCTGTACATGG+TGG - chr8.1:20410732-20410751 None:intergenic 55.0%
! CGGTCTTTCGCCGAAAGTGT+TGG + chr8.1:20410993-20411012 MS.gene48275:CDS 55.0%
! GAGTCGTGGCGCTTTGAATG+CGG + chr8.1:20410954-20410973 MS.gene48275:CDS 55.0%
AAGCCGCTGTACATGGTGGC+TGG - chr8.1:20410728-20410747 None:intergenic 60.0%
CCACATGGACACCAATGGCG+AGG + chr8.1:20410676-20410695 MS.gene48275:CDS 60.0%
GCCACCATGTACAGCGGCTT+AGG + chr8.1:20410728-20410747 MS.gene48275:CDS 60.0%
! CCTCGCCATTGGTGTCCATG+TGG - chr8.1:20410679-20410698 None:intergenic 60.0%
GGTGGCTGGATCGATCTGCG+AGG - chr8.1:20410714-20410733 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 20409857 20411422 20409857 ID=MS.gene48275
chr8.1 mRNA 20409857 20411422 20409857 ID=MS.gene48275.t1;Parent=MS.gene48275
chr8.1 exon 20409857 20411422 20409857 ID=MS.gene48275.t1.exon1;Parent=MS.gene48275.t1
chr8.1 CDS 20409857 20411422 20409857 ID=cds.MS.gene48275.t1;Parent=MS.gene48275.t1
Gene Sequence

>MS.gene48275

ATGTCACCTGCACTTTATGCTAGTACCTTCAAATGTGAGGTAGATGAAAATCCATCATTAATGGGTTCTTACTATGCTTCTTTATATCCAAATTTGCCTATTTTAGAAAACAGTGCTAGTTCAACATGGATCCTTAATCCATTTTCTGATCATGAAACTGAAACCATTAGGGATCACAAGAAATTGAAAAGATCAACCGTAACTATCCCTATTTGGTTTGCGAATTTCAGCAGCCATAGTAATAGTTTCTTCAATAACATCAACAGCAATAACAACAGTGTGAATAGCATTCCAAGGCTTCATTTCAGAGATCACATAAAAACTTATAAGCAAATATATTTTGCTGCTGAAGCTGTTGAAGAAGCAGCAGAAGATAACTTCAACTACAATAATTGCGAAGCTGAAGAAGATGGTTCATGTGCTGATGGAATGAGACTAGTTCAACTTCTAATTGCATGTGCTGAAGCTGTCGCGTGTCGCGACAAATCACACGCTTCGGTTTTATTATCCGAGCTTAAATCAAATGCTTTGGTGTTTGGTTCTTCATTCCAAAGAGTTGCTTCTTGTTTTGTTCAAGGACTCACAGAGAGACTAACTCTGATTCAACCAATTGGTAATAATTCTGCTGGATCAGATACAAAATCAATGATGAACATAATGGATGCTGCATCAGAAGAAATGGAAGAAGCATTCAAGTTGGTTTATGAAAATTGTCCACATATTCAATTTGGACATTTTGTGGCTAATTCCATAATATTGGAAGCCTTTGAGGGTGAGAGTTTTCTTCACGTGGTTGATCTTGGCATGAGCCTTGGTCTTCCACATGGACACCAATGGCGAGGACTAATTCAAAGCCTCGCAGATCGATCCAGCCACCATGTACAGCGGCTTAGGATCACAGCGATTGGTCTATGCATTGCTAGAATCCAAGTCATTGGTGAAGAACTCTCAATTTACGCGAAAAATTTGGGGATTCATTTGGAATTCTCAATTGTTGAGAAGAATTTGGAGAATTTGAAACCTAAGGACATAAAAGTGAATGAAAAAGAGGTCCTTGTGGTTAACAGCATTCTTCAATTGCATTGTGTTGTTAAAGAGAGTCGTGGCGCTTTGAATGCGGTTTTACAAATGATCCACGGTCTTTCGCCGAAAGTGTTGGTTATGGCTGAGCAAGATTCTGGTCACAATGGACCTTTTTTCCTTGGAAGGTTTATGGAATCATTGCATTATTATTCTGCTATATTTGACTCATTGGATGCTATGCTGCCAAAGTATGACACTAAGAGGGCAAAAATGGAACAGTTTTATTTTGCTGAGGAGATAAAGAACATTGTGAGCTGTGAAGGACCATTGAGGATGGAGAGGCATGAGAAAGTTGATCAGTGGAGGAGGAGAATGAGTAGGGCTGGATTTCAAGGTTCACCTATTAAGATGGTTGTTCAAGCTAAGCAGTGGCTTGTGAAGAATAATGTTTGTGATGGATATACTGTTGTGGAAGAGAAAGGGTGTTTGGTTCTTGGATGGAAATCAAAGCCTATTGTTGCAGTTTCTTGCTGGAAATGCTGA
Protein sequence

>MS.gene48275.t1

MSPALYASTFKCEVDENPSLMGSYYASLYPNLPILENSASSTWILNPFSDHETETIRDHKKLKRSTVTIPIWFANFSSHSNSFFNNINSNNNSVNSIPRLHFRDHIKTYKQIYFAAEAVEEAAEDNFNYNNCEAEEDGSCADGMRLVQLLIACAEAVACRDKSHASVLLSELKSNALVFGSSFQRVASCFVQGLTERLTLIQPIGNNSAGSDTKSMMNIMDAASEEMEEAFKLVYENCPHIQFGHFVANSIILEAFEGESFLHVVDLGMSLGLPHGHQWRGLIQSLADRSSHHVQRLRITAIGLCIARIQVIGEELSIYAKNLGIHLEFSIVEKNLENLKPKDIKVNEKEVLVVNSILQLHCVVKESRGALNAVLQMIHGLSPKVLVMAEQDSGHNGPFFLGRFMESLHYYSAIFDSLDAMLPKYDTKRAKMEQFYFAEEIKNIVSCEGPLRMERHEKVDQWRRRMSRAGFQGSPIKMVVQAKQWLVKNNVCDGYTVVEEKGCLVLGWKSKPIVAVSCWKC