Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48603.t1 | PNY07851.1 | 35.2 | 216 | 138 | 2 | 11 | 224 | 9 | 224 | 1.30E-31 | 146.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48603.t1 | A0A2K3NXT8 | 35.2 | 216 | 138 | 2 | 11 | 224 | 9 | 224 | 9.0e-32 | 146.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene48603.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48603.t1 | MTR_4g123470 | 28.796 | 191 | 136 | 0 | 19 | 209 | 20 | 210 | 1.19e-26 | 102 |
| MS.gene48603.t1 | MTR_1g054495 | 23.188 | 207 | 158 | 1 | 18 | 224 | 16 | 221 | 4.50e-15 | 72.0 |
| MS.gene48603.t1 | MTR_4g085790 | 24.103 | 195 | 147 | 1 | 30 | 224 | 28 | 221 | 9.97e-14 | 68.2 |
| MS.gene48603.t1 | MTR_7g025150 | 23.590 | 195 | 148 | 1 | 30 | 224 | 28 | 221 | 1.11e-13 | 68.2 |
| MS.gene48603.t1 | MTR_0014s0220 | 26.203 | 187 | 121 | 4 | 28 | 214 | 644 | 813 | 1.20e-13 | 69.7 |
| MS.gene48603.t1 | MTR_4g053550 | 45.714 | 70 | 38 | 0 | 28 | 97 | 49 | 118 | 1.69e-13 | 65.5 |
| MS.gene48603.t1 | MTR_1g090753 | 32.632 | 95 | 64 | 0 | 23 | 117 | 38 | 132 | 7.78e-13 | 64.7 |
| MS.gene48603.t1 | MTR_5g074790 | 31.731 | 104 | 71 | 0 | 15 | 118 | 160 | 263 | 8.47e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 50 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAATGTGATGTACTAATA+AGG | 0.256110 | 4.1:-66873661 | MS.gene48603:CDS |
| GAACTTAACTTTGTGTCTAT+TGG | 0.280740 | 4.1:+66874093 | None:intergenic |
| CAGATGTAATGAAGGTTTCT+AGG | 0.299323 | 4.1:-66873526 | MS.gene48603:CDS |
| TTCCAATTCACTGCATTTCA+TGG | 0.313981 | 4.1:-66873959 | MS.gene48603:CDS |
| TGATAACCTGCAGCCATATA+AGG | 0.324757 | 4.1:+66873851 | None:intergenic |
| AATGACCTTAACTCCTTATA+TGG | 0.332349 | 4.1:-66873864 | MS.gene48603:CDS |
| TAAATTCTCTTCCAAGTTTG+TGG | 0.333428 | 4.1:+66874134 | None:intergenic |
| CTGAGGGATGGATCAACTTA+AGG | 0.380199 | 4.1:-66874042 | MS.gene48603:CDS |
| GTCCATGAAATGCAGTGAAT+TGG | 0.385634 | 4.1:+66873957 | None:intergenic |
| AGGACTAAACATAGGGATAA+TGG | 0.397283 | 4.1:-66874308 | MS.gene48603:CDS |
| TAAGCTTAAGTTTAAGTTTG+TGG | 0.399998 | 4.1:-66873561 | MS.gene48603:CDS |
| GGTGATTACATTAGAAGCTC+AGG | 0.426576 | 4.1:-66873725 | MS.gene48603:CDS |
| CAGACACTGAAGATTGACTT+TGG | 0.437142 | 4.1:-66873746 | MS.gene48603:CDS |
| CAAATGTGATGTACTAATAA+GGG | 0.442101 | 4.1:-66873660 | MS.gene48603:CDS |
| TGCAGCCATATAAGGAGTTA+AGG | 0.444382 | 4.1:+66873859 | None:intergenic |
| TATGGCTGCAGGTTATCAAT+TGG | 0.445570 | 4.1:-66873846 | MS.gene48603:intron |
| GAGTTAAGGTCATTTCATAA+TGG | 0.446526 | 4.1:+66873873 | None:intergenic |
| TACTCTGCTATTAATATGTA+TGG | 0.453609 | 4.1:-66873701 | MS.gene48603:CDS |
| GTTGTTATCAATACAATGTC+TGG | 0.455307 | 4.1:-66874347 | None:intergenic |
| ATTAATATGTATGGACCTAC+AGG | 0.484413 | 4.1:-66873692 | MS.gene48603:CDS |
| TGAGGGATGGATCAACTTAA+GGG | 0.485967 | 4.1:-66874041 | MS.gene48603:CDS |
| CTGGTGGTGGATCAACTTCA+AGG | 0.486585 | 4.1:-66874328 | MS.gene48603:CDS |
| TTAACTCCTTATATGGCTGC+AGG | 0.501456 | 4.1:-66873857 | MS.gene48603:CDS |
| TGTAAAGACAATGGATTCCA+TGG | 0.503947 | 4.1:-66873590 | MS.gene48603:CDS |
| ATAAAATGCTCAACAAATGA+AGG | 0.510176 | 4.1:+66874157 | None:intergenic |
| CAACTTCAAGGACTAAACAT+AGG | 0.510435 | 4.1:-66874316 | MS.gene48603:CDS |
| CACTTACAAGCAAGGAATTG+AGG | 0.514420 | 4.1:-66873903 | MS.gene48603:CDS |
| TGCTTGTAAGTGTGCGATGA+TGG | 0.527718 | 4.1:+66873914 | None:intergenic |
| ATCCATCCCTCAGTCAGAAG+TGG | 0.551673 | 4.1:+66874052 | None:intergenic |
| ATATCTCATTTACAGCCGTA+TGG | 0.553596 | 4.1:-66874190 | MS.gene48603:intron |
| AAGACAATGGATTCCATGGT+GGG | 0.554852 | 4.1:-66873586 | MS.gene48603:CDS |
| AAGTACATCAGATGTAATGA+AGG | 0.558515 | 4.1:-66873534 | MS.gene48603:CDS |
| ATCAATACAATGTCTGGTGG+TGG | 0.560856 | 4.1:-66874341 | None:intergenic |
| ATCCACTTCTGACTGAGGGA+TGG | 0.571896 | 4.1:-66874054 | MS.gene48603:CDS |
| AAGGCAACAAAGATTGGCAG+TGG | 0.574230 | 4.1:-66873626 | MS.gene48603:CDS |
| AACTTCAAGGACTAAACATA+GGG | 0.584439 | 4.1:-66874315 | MS.gene48603:CDS |
| CATTTGTACTCGGTGCCTGT+AGG | 0.588159 | 4.1:+66873677 | None:intergenic |
| TAGTACATCACATTTGTACT+CGG | 0.590498 | 4.1:+66873667 | None:intergenic |
| TGTGATGTACTAATAAGGGA+TGG | 0.597036 | 4.1:-66873656 | MS.gene48603:CDS |
| TGAACATCCACTTCTGACTG+AGG | 0.599795 | 4.1:-66874059 | MS.gene48603:CDS |
| CAACAAAGATTGGCAGTGGG+TGG | 0.601376 | 4.1:-66873622 | MS.gene48603:CDS |
| TACTGCTTATATCATCAATG+AGG | 0.608119 | 4.1:-66874276 | MS.gene48603:intron |
| GAAGGTCTGATCTCTCCATA+CGG | 0.615737 | 4.1:+66874175 | None:intergenic |
| ACTTAAGCTTATCCCCACCA+TGG | 0.632346 | 4.1:+66873573 | None:intergenic |
| AAAGACAATGGATTCCATGG+TGG | 0.635851 | 4.1:-66873587 | MS.gene48603:CDS |
| AGACAATGGATTCCATGGTG+GGG | 0.640570 | 4.1:-66873585 | MS.gene48603:CDS |
| TCATCGCACACTTACAAGCA+AGG | 0.641070 | 4.1:-66873911 | MS.gene48603:CDS |
| GAACATCCACTTCTGACTGA+GGG | 0.646274 | 4.1:-66874058 | MS.gene48603:CDS |
| AGGCAACAAAGATTGGCAGT+GGG | 0.670518 | 4.1:-66873625 | MS.gene48603:CDS |
| GTTATCAATACAATGTCTGG+TGG | 0.684260 | 4.1:-66874344 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATGATTTTTATAAAGTTCT+TGG | - | chr4.1:66873859-66873878 | MS.gene48603:CDS | 15.0% |
| !!! | ATGATTTTTATAAAGTTCTT+GGG | - | chr4.1:66873860-66873879 | MS.gene48603:CDS | 15.0% |
| !! | ATTGCTCAACATAAAATAAT+AGG | + | chr4.1:66873611-66873630 | None:intergenic | 20.0% |
| !!! | AACAAATTTTGTAAAGACAA+TGG | - | chr4.1:66874253-66874272 | MS.gene48603:intron | 20.0% |
| !!! | TGATTTTTATAAAGTTCTTG+GGG | - | chr4.1:66873861-66873880 | MS.gene48603:CDS | 20.0% |
| ! | ACAAATGTGATGTACTAATA+AGG | - | chr4.1:66874191-66874210 | MS.gene48603:intron | 25.0% |
| ! | ATAAAATGCTCAACAAATGA+AGG | + | chr4.1:66873698-66873717 | None:intergenic | 25.0% |
| ! | CAAATGTGATGTACTAATAA+GGG | - | chr4.1:66874192-66874211 | MS.gene48603:intron | 25.0% |
| ! | TACTCTGCTATTAATATGTA+TGG | - | chr4.1:66874151-66874170 | MS.gene48603:CDS | 25.0% |
| ! | TAGAGATGTATCATTTGATA+AGG | + | chr4.1:66874047-66874066 | None:intergenic | 25.0% |
| !! | TAAGCTTAAGTTTAAGTTTG+TGG | - | chr4.1:66874291-66874310 | MS.gene48603:CDS | 25.0% |
| AAAAAGAAGGCAACAAAGAT+TGG | - | chr4.1:66874220-66874239 | MS.gene48603:intron | 30.0% | |
| AACTTCAAGGACTAAACATA+GGG | - | chr4.1:66873537-66873556 | MS.gene48603:CDS | 30.0% | |
| AAGTACATCAGATGTAATGA+AGG | - | chr4.1:66874318-66874337 | MS.gene48603:CDS | 30.0% | |
| AATAAGGGATGGAAAAAAGA+AGG | - | chr4.1:66874207-66874226 | MS.gene48603:intron | 30.0% | |
| AATGACCTTAACTCCTTATA+TGG | - | chr4.1:66873988-66874007 | MS.gene48603:CDS | 30.0% | |
| ATCATTTGATAAGGAGTTGT+TGG | + | chr4.1:66874038-66874057 | None:intergenic | 30.0% | |
| ATTAATATGTATGGACCTAC+AGG | - | chr4.1:66874160-66874179 | MS.gene48603:CDS | 30.0% | |
| GAACTTAACTTTGTGTCTAT+TGG | + | chr4.1:66873762-66873781 | None:intergenic | 30.0% | |
| GAGTTAAGGTCATTTCATAA+TGG | + | chr4.1:66873982-66874001 | None:intergenic | 30.0% | |
| TAAATTCTCTTCCAAGTTTG+TGG | + | chr4.1:66873721-66873740 | None:intergenic | 30.0% | |
| TACTGCTTATATCATCAATG+AGG | - | chr4.1:66873576-66873595 | MS.gene48603:CDS | 30.0% | |
| TAGTACATCACATTTGTACT+CGG | + | chr4.1:66874188-66874207 | None:intergenic | 30.0% | |
| ! | ATCAACTTAAGGGAGTTTTA+TGG | - | chr4.1:66873821-66873840 | MS.gene48603:intron | 30.0% |
| AGGACTAAACATAGGGATAA+TGG | - | chr4.1:66873544-66873563 | MS.gene48603:CDS | 35.0% | |
| ATATCTCATTTACAGCCGTA+TGG | - | chr4.1:66873662-66873681 | MS.gene48603:CDS | 35.0% | |
| CAACTTCAAGGACTAAACAT+AGG | - | chr4.1:66873536-66873555 | MS.gene48603:CDS | 35.0% | |
| CAGATGTAATGAAGGTTTCT+AGG | - | chr4.1:66874326-66874345 | MS.gene48603:CDS | 35.0% | |
| TGTGATGTACTAATAAGGGA+TGG | - | chr4.1:66874196-66874215 | MS.gene48603:intron | 35.0% | |
| TTCCAATTCACTGCATTTCA+TGG | - | chr4.1:66873893-66873912 | MS.gene48603:CDS | 35.0% | |
| ! | GAGCATTTTATCCACAAACT+TGG | - | chr4.1:66873707-66873726 | MS.gene48603:CDS | 35.0% |
| ! | TGTAAAGACAATGGATTCCA+TGG | - | chr4.1:66874262-66874281 | MS.gene48603:intron | 35.0% |
| !! | ACTTAAGGGAGTTTTATGGT+TGG | - | chr4.1:66873825-66873844 | MS.gene48603:intron | 35.0% |
| CACTTACAAGCAAGGAATTG+AGG | - | chr4.1:66873949-66873968 | MS.gene48603:CDS | 40.0% | |
| GGTGATTACATTAGAAGCTC+AGG | - | chr4.1:66874127-66874146 | MS.gene48603:CDS | 40.0% | |
| GTCCATGAAATGCAGTGAAT+TGG | + | chr4.1:66873898-66873917 | None:intergenic | 40.0% | |
| TATGGCTGCAGGTTATCAAT+TGG | - | chr4.1:66874006-66874025 | MS.gene48603:CDS | 40.0% | |
| TGAGGGATGGATCAACTTAA+GGG | - | chr4.1:66873811-66873830 | MS.gene48603:intron | 40.0% | |
| TGATAACCTGCAGCCATATA+AGG | + | chr4.1:66874004-66874023 | None:intergenic | 40.0% | |
| TGCAGCCATATAAGGAGTTA+AGG | + | chr4.1:66873996-66874015 | None:intergenic | 40.0% | |
| TTAACTCCTTATATGGCTGC+AGG | - | chr4.1:66873995-66874014 | MS.gene48603:CDS | 40.0% | |
| ! | AAAGACAATGGATTCCATGG+TGG | - | chr4.1:66874265-66874284 | MS.gene48603:intron | 40.0% |
| ! | AAGACAATGGATTCCATGGT+GGG | - | chr4.1:66874266-66874285 | MS.gene48603:intron | 40.0% |
| ! | CAGACACTGAAGATTGACTT+TGG | - | chr4.1:66874106-66874125 | MS.gene48603:CDS | 40.0% |
| AAGGCAACAAAGATTGGCAG+TGG | - | chr4.1:66874226-66874245 | MS.gene48603:intron | 45.0% | |
| ACTTAAGCTTATCCCCACCA+TGG | + | chr4.1:66874282-66874301 | None:intergenic | 45.0% | |
| AGGCAACAAAGATTGGCAGT+GGG | - | chr4.1:66874227-66874246 | MS.gene48603:intron | 45.0% | |
| CTGAGGGATGGATCAACTTA+AGG | - | chr4.1:66873810-66873829 | MS.gene48603:intron | 45.0% | |
| GAACATCCACTTCTGACTGA+GGG | - | chr4.1:66873794-66873813 | MS.gene48603:intron | 45.0% | |
| GAAGGTCTGATCTCTCCATA+CGG | + | chr4.1:66873680-66873699 | None:intergenic | 45.0% | |
| TCATCGCACACTTACAAGCA+AGG | - | chr4.1:66873941-66873960 | MS.gene48603:CDS | 45.0% | |
| TGAACATCCACTTCTGACTG+AGG | - | chr4.1:66873793-66873812 | MS.gene48603:intron | 45.0% | |
| TGCTTGTAAGTGTGCGATGA+TGG | + | chr4.1:66873941-66873960 | None:intergenic | 45.0% | |
| ! | AGACAATGGATTCCATGGTG+GGG | - | chr4.1:66874267-66874286 | MS.gene48603:intron | 45.0% |
| ATCCACTTCTGACTGAGGGA+TGG | - | chr4.1:66873798-66873817 | MS.gene48603:intron | 50.0% | |
| ATCCATCCCTCAGTCAGAAG+TGG | + | chr4.1:66873803-66873822 | None:intergenic | 50.0% | |
| CAACAAAGATTGGCAGTGGG+TGG | - | chr4.1:66874230-66874249 | MS.gene48603:intron | 50.0% | |
| !! | CATTTGTACTCGGTGCCTGT+AGG | + | chr4.1:66874178-66874197 | None:intergenic | 50.0% |
| !! | CTGGTGGTGGATCAACTTCA+AGG | - | chr4.1:66873524-66873543 | MS.gene48603:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 66873520 | 66874354 | 66873520 | ID=MS.gene48603 |
| chr4.1 | mRNA | 66873520 | 66874354 | 66873520 | ID=MS.gene48603.t1;Parent=MS.gene48603 |
| chr4.1 | exon | 66874277 | 66874354 | 66874277 | ID=MS.gene48603.t1.exon1;Parent=MS.gene48603.t1 |
| chr4.1 | CDS | 66874277 | 66874354 | 66874277 | ID=cds.MS.gene48603.t1;Parent=MS.gene48603.t1 |
| chr4.1 | exon | 66873847 | 66874197 | 66873847 | ID=MS.gene48603.t1.exon2;Parent=MS.gene48603.t1 |
| chr4.1 | CDS | 66873847 | 66874197 | 66873847 | ID=cds.MS.gene48603.t1;Parent=MS.gene48603.t1 |
| chr4.1 | exon | 66873520 | 66873765 | 66873520 | ID=MS.gene48603.t1.exon3;Parent=MS.gene48603.t1 |
| chr4.1 | CDS | 66873520 | 66873765 | 66873520 | ID=cds.MS.gene48603.t1;Parent=MS.gene48603.t1 |
| Gene Sequence |
| Protein sequence |
