AlfalfaGEDB | MS.gene48715

M. sativa cultivar XinJiangDaYe / MS.gene48715

KEGG: -
GO: GO:0000976 GO:0000981 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0035326 GO:0043565 GO:0044212 GO:0045893 GO:0045935 GO:0045944 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48715.t1	XP_003605525.1	79.8	99	20	0	1	99	1	99	2.20E-36	161.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48715.t1	Q9FJK3	58.0	100	42	0	1	100	1	100	3.9e-29	128.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48715.t1	G7JN85	79.8	99	20	0	1	99	1	99	1.6e-36	161.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene48715.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48715.t1	MTR_4g032620	79.798	99	20	0	1	99	1	99	1.01e-53	167
MS.gene48715.t1	MTR_8g036130	65.347	101	33	1	1	99	1	101	4.76e-44	143
MS.gene48715.t1	MTR_2g035610	65.657	99	34	0	1	99	1	99	6.32e-40	129
MS.gene48715.t1	MTR_3g065100	63.636	99	36	0	1	99	1	99	1.67e-38	129
MS.gene48715.t1	MTR_4g031910	64.646	99	35	0	1	99	1	99	9.06e-38	126
MS.gene48715.t1	MTR_3g466980	53.535	99	46	0	1	99	1	99	1.23e-37	126
MS.gene48715.t1	MTR_4g028800	76.712	73	15	1	1	73	1	71	4.04e-37	120
MS.gene48715.t1	MTR_3g466830	52.083	96	46	0	4	99	3	98	3.78e-36	120
MS.gene48715.t1	MTR_4g032260	65.657	99	34	0	1	99	1	99	1.06e-35	119
MS.gene48715.t1	MTR_4g063790	51.042	96	47	0	1	96	1	96	8.38e-34	115
MS.gene48715.t1	MTR_4g032290	62.626	99	37	0	1	99	1	99	1.27e-33	116
MS.gene48715.t1	MTR_2g016210	48.980	98	50	0	1	98	1	98	5.38e-32	112
MS.gene48715.t1	MTR_5g075380	47.475	99	52	0	1	99	1	99	6.34e-32	110
MS.gene48715.t1	MTR_3g466890	46.316	95	51	0	5	99	4	98	4.42e-31	107
MS.gene48715.t1	MTR_3g467080	45.361	97	53	0	1	97	1	97	7.87e-31	107
MS.gene48715.t1	MTR_3g031240	42.424	99	57	0	1	99	1	99	5.98e-30	105
MS.gene48715.t1	MTR_5g047580	43.299	97	54	1	1	97	1	96	1.42e-28	101
MS.gene48715.t1	MTR_1g077390	44.330	97	53	1	1	97	1	96	1.33e-27	99.0
MS.gene48715.t1	MTR_1g090783	43.750	96	53	1	4	99	3	97	2.86e-27	97.8
MS.gene48715.t1	MTR_1g090710	40.404	99	58	1	1	99	1	98	4.58e-27	97.4
MS.gene48715.t1	MTR_3g031100	42.424	99	57	0	1	99	1	99	3.78e-26	97.4
MS.gene48715.t1	MTR_1g090697	42.424	99	56	1	1	99	1	98	6.67e-26	94.4
MS.gene48715.t1	MTR_2g035580	36.842	95	60	0	1	95	1	95	2.52e-24	90.5
MS.gene48715.t1	MTR_7g106510	43.158	95	54	0	4	98	7	101	1.61e-23	93.6
MS.gene48715.t1	MTR_3g465410	50.725	69	34	0	29	97	1	69	9.24e-23	85.9
MS.gene48715.t1	MTR_4g019670	41.053	95	56	0	4	98	7	101	9.90e-23	91.3
MS.gene48715.t1	MTR_1g084950	43.299	97	54	1	1	97	1	96	1.35e-21	83.6
MS.gene48715.t1	MTR_7g055800	46.316	95	23	2	1	95	1	67	2.68e-21	80.9
MS.gene48715.t1	MTR_7g011950	39.394	99	59	1	1	99	1	98	8.47e-21	81.6
MS.gene48715.t1	MTR_7g055940	45.263	95	24	2	1	95	1	67	1.64e-20	78.6
MS.gene48715.t1	MTR_1g077320	38.384	99	60	1	1	99	1	98	1.31e-19	78.6
MS.gene48715.t1	MTR_1g077300	43.750	96	53	1	1	96	1	95	3.53e-19	77.4
MS.gene48715.t1	MTR_7g055790	46.465	99	35	1	1	99	59	139	3.58e-19	77.0
MS.gene48715.t1	MTR_3g093900	37.778	90	56	0	4	93	7	96	3.76e-19	81.3
MS.gene48715.t1	MTR_4g028720	40.206	97	57	1	1	97	1	96	1.48e-18	75.9
MS.gene48715.t1	MTR_3g466930	39.706	68	41	0	32	99	2	69	2.60e-18	74.3
MS.gene48715.t1	MTR_3g466900	38.235	68	42	0	32	99	2	69	5.49e-17	70.9
MS.gene48715.t1	MTR_5g047560	34.021	97	47	2	1	97	1	80	1.37e-15	67.8
MS.gene48715.t1	MTR_1g114730	37.000	100	56	3	5	98	5	103	3.04e-15	69.7
MS.gene48715.t1	MTR_6g018920	38.000	100	55	4	5	98	5	103	1.42e-14	67.8
MS.gene48715.t1	MTR_6g005450	37.000	100	56	3	5	98	5	103	2.50e-13	64.3
MS.gene48715.t1	MTR_6g005440	37.000	100	56	3	5	98	5	103	1.17e-12	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48715.t1	AT3G05860	45.545	101	55	0	1	101	1	101	1.67e-32	112
MS.gene48715.t1	AT3G05860	45.545	101	55	0	1	101	1	101	9.01e-32	112
MS.gene48715.t1	AT3G05860	45.545	101	55	0	1	101	1	101	1.31e-31	112
MS.gene48715.t1	AT5G48670	58.000	100	42	0	1	100	1	100	3.19e-31	112
MS.gene48715.t1	AT5G26650	48.958	96	49	0	4	99	2	97	3.62e-29	107
MS.gene48715.t1	AT1G65300	51.613	93	45	0	4	96	3	95	6.15e-29	105
MS.gene48715.t1	AT5G27960	47.917	96	50	0	4	99	2	97	3.92e-28	103
MS.gene48715.t1	AT1G65330	48.913	92	47	0	5	96	4	95	2.65e-27	101
MS.gene48715.t1	AT5G27810	52.941	68	32	0	29	96	1	68	1.24e-23	87.8
MS.gene48715.t1	AT1G31640	42.105	95	54	1	5	99	4	97	6.51e-23	91.7
MS.gene48715.t1	AT1G31630	42.268	97	53	2	5	101	4	97	1.26e-22	89.7
MS.gene48715.t1	AT5G26630	45.000	100	55	0	1	100	1	100	4.97e-22	86.3
MS.gene48715.t1	AT2G28700	37.755	98	61	0	1	98	1	98	1.67e-21	86.7
MS.gene48715.t1	AT5G06500	41.176	102	60	0	1	102	1	102	1.83e-21	85.1
MS.gene48715.t1	AT5G26580	40.404	99	45	1	1	99	1	85	9.67e-21	84.7
MS.gene48715.t1	AT1G22590	33.673	98	65	0	1	98	1	98	1.23e-20	81.3
MS.gene48715.t1	AT2G40210	39.394	99	60	0	1	99	1	99	1.64e-19	81.6
MS.gene48715.t1	AT5G58890	37.374	99	54	3	1	92	1	98	2.12e-13	64.3
MS.gene48715.t1	AT5G55690	35.185	108	57	3	1	101	1	102	4.67e-13	63.2
MS.gene48715.t1	AT5G55690	35.185	108	57	3	1	101	1	102	4.67e-13	63.2

Find 28 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGCTTGTGCAGTAGTTTA+TGG	0.155587	4.4:-72054744	MS.gene48715:CDS
CACAACAATGACTGGGAAAA+AGG	0.190222	4.4:-72054874	None:intergenic
TCATTGTCGATGATGATTCT+AGG	0.260008	4.4:-72054839	MS.gene48715:CDS
AGATCTCTGGTTGAGGCTCA+TGG	0.361064	4.4:+72054718	None:intergenic
TGTGGTGATGGCCAGATCTC+TGG	0.397343	4.4:+72054705	None:intergenic
GGAAAGCAACATACAATAGA+AGG	0.409123	4.4:-72054818	MS.gene48715:CDS
GATCTCTGGTTGAGGCTCAT+GGG	0.428787	4.4:+72054719	None:intergenic
TCAAAATTCAGAACAATGAC+TGG	0.434871	4.4:-72054663	MS.gene48715:CDS
AAGAGACTTACATGAAAGAA+AGG	0.463147	4.4:-72054611	MS.gene48715:CDS
ATGAGCCTCAACCAGAGATC+TGG	0.467494	4.4:-72054716	MS.gene48715:CDS
ATCTCTGGTTGAGGCTCATG+GGG	0.492174	4.4:+72054720	None:intergenic
AGAGATCTGGCCATCACCAC+AGG	0.494291	4.4:-72054703	MS.gene48715:CDS
ATCACCACAGGGAGTTCAAA+CGG	0.524260	4.4:-72054691	MS.gene48715:CDS
AGAATCATCATCGACAATGA+AGG	0.542377	4.4:+72054842	None:intergenic
GATGGCCAGATCTCTGGTTG+AGG	0.555241	4.4:+72054711	None:intergenic
ATTCAGAACAATGACTGGGC+GGG	0.563336	4.4:-72054658	MS.gene48715:CDS
AACATACAATAGAAGGCGGA+AGG	0.566279	4.4:-72054811	MS.gene48715:CDS
GTTTGAACTCCCTGTGGTGA+TGG	0.567517	4.4:+72054693	None:intergenic
AGAGACTTACATGAAAGAAA+GGG	0.577279	4.4:-72054610	MS.gene48715:CDS
TATGAACTAAGCACTTTGTG+TGG	0.579542	4.4:-72054771	MS.gene48715:CDS
GCGGAAGGGTCTAATGAAGA+AGG	0.591410	4.4:-72054796	MS.gene48715:CDS
CAAAATTCAGAACAATGACT+GGG	0.592691	4.4:-72054662	MS.gene48715:CDS
GCGGGAGAAGAGCAAGAAAA+CGG	0.596443	4.4:-72054640	MS.gene48715:CDS
ACATACAATAGAAGGCGGAA+GGG	0.677825	4.4:-72054810	MS.gene48715:CDS
GAGATCTGGCCATCACCACA+GGG	0.678612	4.4:-72054702	MS.gene48715:CDS
AATTCAGAACAATGACTGGG+CGG	0.687254	4.4:-72054659	MS.gene48715:CDS
AAGCAACATACAATAGAAGG+CGG	0.694844	4.4:-72054815	MS.gene48715:CDS
AGCACCGTTTGAACTCCCTG+TGG	0.787870	4.4:+72054687	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCTAAAAACTGAAGATACAA+AGG	-	chr4.4:72054862-72054881	MS.gene48715:CDS	25.0%
	AAGAGACTTACATGAAAGAA+AGG	-	chr4.4:72054837-72054856	MS.gene48715:CDS	30.0%
	AGAGACTTACATGAAAGAAA+GGG	-	chr4.4:72054838-72054857	MS.gene48715:CDS	30.0%
	CAAAATTCAGAACAATGACT+GGG	-	chr4.4:72054786-72054805	MS.gene48715:CDS	30.0%
	TCAAAATTCAGAACAATGAC+TGG	-	chr4.4:72054785-72054804	MS.gene48715:CDS	30.0%
	AAGCAACATACAATAGAAGG+CGG	-	chr4.4:72054633-72054652	MS.gene48715:CDS	35.0%
	AGAATCATCATCGACAATGA+AGG	+	chr4.4:72054609-72054628	None:intergenic	35.0%
	GGAAAGCAACATACAATAGA+AGG	-	chr4.4:72054630-72054649	MS.gene48715:CDS	35.0%
	TATGAACTAAGCACTTTGTG+TGG	-	chr4.4:72054677-72054696	MS.gene48715:CDS	35.0%
	TCATTGTCGATGATGATTCT+AGG	-	chr4.4:72054609-72054628	MS.gene48715:CDS	35.0%
	AACATACAATAGAAGGCGGA+AGG	-	chr4.4:72054637-72054656	MS.gene48715:CDS	40.0%
	AATTCAGAACAATGACTGGG+CGG	-	chr4.4:72054789-72054808	MS.gene48715:CDS	40.0%
	ACATACAATAGAAGGCGGAA+GGG	-	chr4.4:72054638-72054657	MS.gene48715:CDS	40.0%
	GAAGCTTGTGCAGTAGTTTA+TGG	-	chr4.4:72054704-72054723	MS.gene48715:CDS	40.0%
	ATCACCACAGGGAGTTCAAA+CGG	-	chr4.4:72054757-72054776	MS.gene48715:CDS	45.0%
	ATTCAGAACAATGACTGGGC+GGG	-	chr4.4:72054790-72054809	MS.gene48715:CDS	45.0%
	AGATCTCTGGTTGAGGCTCA+TGG	+	chr4.4:72054733-72054752	None:intergenic	50.0%
	ATCTCTGGTTGAGGCTCATG+GGG	+	chr4.4:72054731-72054750	None:intergenic	50.0%
	ATGAGCCTCAACCAGAGATC+TGG	-	chr4.4:72054732-72054751	MS.gene48715:CDS	50.0%
	GATCTCTGGTTGAGGCTCAT+GGG	+	chr4.4:72054732-72054751	None:intergenic	50.0%
	GCGGAAGGGTCTAATGAAGA+AGG	-	chr4.4:72054652-72054671	MS.gene48715:CDS	50.0%
	GCGGGAGAAGAGCAAGAAAA+CGG	-	chr4.4:72054808-72054827	MS.gene48715:CDS	50.0%
	GTTTGAACTCCCTGTGGTGA+TGG	+	chr4.4:72054758-72054777	None:intergenic	50.0%
	AGAGATCTGGCCATCACCAC+AGG	-	chr4.4:72054745-72054764	MS.gene48715:CDS	55.0%
	AGCACCGTTTGAACTCCCTG+TGG	+	chr4.4:72054764-72054783	None:intergenic	55.0%
	GAGATCTGGCCATCACCACA+GGG	-	chr4.4:72054746-72054765	MS.gene48715:CDS	55.0%
	GATGGCCAGATCTCTGGTTG+AGG	+	chr4.4:72054740-72054759	None:intergenic	55.0%
!!	TGTGGTGATGGCCAGATCTC+TGG	+	chr4.4:72054746-72054765	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	72054581	72054889	72054581	ID=MS.gene48715
chr4.4	mRNA	72054581	72054889	72054581	ID=MS.gene48715.t1;Parent=MS.gene48715
chr4.4	exon	72054581	72054889	72054581	ID=MS.gene48715.t1.exon1;Parent=MS.gene48715.t1
chr4.4	CDS	72054581	72054889	72054581	ID=cds.MS.gene48715.t1;Parent=MS.gene48715.t1

Gene Sequence	>MS.gene48715 ATGACTGGGAAAAAGGTGAAACTTGCCTTCATTGTCGATGATGATTCTAGGAAAGCAACATACAATAGAAGGCGGAAGGGTCTAATGAAGAAGGTTTATGAACTAAGCACTTTGTGTGGAGTTGAAGCTTGTGCAGTAGTTTATGGACCCCATGAGCCTCAACCAGAGATCTGGCCATCACCACAGGGAGTTCAAACGGTGCTCTCAAAATTCAGAACAATGACTGGGCGGGAGAAGAGCAAGAAAACGGTTGATCAAGAGACTTACATGAAAGAAAGGGTTCTAAAAACTGAAGATACAAAGGCATGA
Protein sequence	>MS.gene48715.t1 MTGKKVKLAFIVDDDSRKATYNRRRKGLMKKVYELSTLCGVEACAVVYGPHEPQPEIWPSPQGVQTVLSKFRTMTGREKSKKTVDQETYMKERVLKTEDTKA