AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48720.t1 MTR_4g031910 72.650 234 47 2 1 222 1 229 7.08e-114 325
MS.gene48720.t1 MTR_4g032290 68.852 244 41 2 1 222 1 231 5.95e-109 312
MS.gene48720.t1 MTR_4g032620 58.442 231 87 1 1 222 1 231 5.35e-95 277
MS.gene48720.t1 MTR_8g036130 56.680 247 82 4 1 222 1 247 4.71e-88 260
MS.gene48720.t1 MTR_4g032260 79.618 157 32 0 1 157 1 157 4.55e-80 236
MS.gene48720.t1 MTR_3g065100 49.378 241 97 4 1 219 1 238 1.96e-65 202
MS.gene48720.t1 MTR_3g466980 45.690 232 101 5 1 218 1 221 1.28e-63 197
MS.gene48720.t1 MTR_2g035610 72.951 122 33 0 1 122 1 122 8.71e-52 164
MS.gene48720.t1 MTR_4g032270 56.204 137 45 2 94 222 2 131 1.66e-46 150
MS.gene48720.t1 MTR_5g075380 47.742 155 80 1 1 155 1 154 5.49e-46 150
MS.gene48720.t1 MTR_4g063790 45.455 154 84 0 1 154 1 154 1.46e-45 149
MS.gene48720.t1 MTR_3g466830 48.344 151 77 1 3 153 2 151 4.09e-44 145
MS.gene48720.t1 MTR_3g467080 43.590 156 88 0 1 156 1 156 2.32e-43 143
MS.gene48720.t1 MTR_3g031100 35.268 224 133 4 1 219 1 217 5.64e-43 145
MS.gene48720.t1 MTR_5g047580 46.154 156 82 2 1 156 1 154 6.96e-41 137
MS.gene48720.t1 MTR_2g016210 39.560 182 108 2 1 180 1 182 8.38e-41 139
MS.gene48720.t1 MTR_3g466890 45.395 152 82 1 3 154 2 152 4.16e-39 132
MS.gene48720.t1 MTR_1g077390 42.308 156 88 2 1 156 1 154 2.18e-37 128
MS.gene48720.t1 MTR_1g090783 42.208 154 87 2 3 156 2 153 1.21e-35 124
MS.gene48720.t1 MTR_3g465410 44.444 126 70 0 29 154 1 126 6.03e-35 121
MS.gene48720.t1 MTR_1g077320 44.231 156 85 2 1 156 1 154 8.32e-35 121
MS.gene48720.t1 MTR_7g011950 43.590 156 86 2 1 156 1 154 2.53e-34 120
MS.gene48720.t1 MTR_4g028720 44.231 156 85 2 1 156 1 154 4.02e-34 120
MS.gene48720.t1 MTR_1g084950 44.872 156 84 2 1 156 1 154 1.82e-33 118
MS.gene48720.t1 MTR_1g077300 43.590 156 84 2 1 156 1 152 2.71e-32 115
MS.gene48720.t1 MTR_1g090710 38.312 154 94 1 1 154 1 153 6.26e-32 114
MS.gene48720.t1 MTR_1g090697 40.000 150 88 2 1 150 1 148 2.66e-31 112
MS.gene48720.t1 MTR_4g028800 75.714 70 15 1 1 70 1 68 4.99e-30 106
MS.gene48720.t1 MTR_3g031240 33.974 156 103 0 1 156 1 156 3.15e-29 108
MS.gene48720.t1 MTR_7g055800 49.573 117 31 2 1 117 1 89 9.51e-28 101
MS.gene48720.t1 MTR_3g466900 40.650 123 72 1 32 154 2 123 4.97e-27 100
MS.gene48720.t1 MTR_2g035580 38.217 157 96 1 1 157 1 156 4.46e-26 99.4
MS.gene48720.t1 MTR_7g055790 52.252 111 35 1 1 111 59 151 5.93e-26 98.6
MS.gene48720.t1 MTR_3g466930 38.211 123 75 1 32 154 2 123 1.24e-24 94.7
MS.gene48720.t1 MTR_7g106510 38.583 127 78 0 3 129 6 132 2.01e-23 97.8
MS.gene48720.t1 MTR_4g019670 39.370 127 77 0 3 129 6 132 3.42e-23 97.4
MS.gene48720.t1 MTR_5g047560 36.538 156 81 3 1 156 1 138 3.68e-23 91.3
MS.gene48720.t1 MTR_7g055940 48.113 106 27 2 1 106 1 78 1.41e-22 87.8
MS.gene48720.t1 MTR_3g093900 30.986 142 98 0 3 144 6 147 1.59e-20 89.7
MS.gene48720.t1 MTR_2g035590 58.025 81 34 0 74 154 1 81 1.62e-19 80.5
MS.gene48720.t1 MTR_1g114730 31.858 113 70 3 1 107 1 112 1.90e-13 68.6
MS.gene48720.t1 MTR_6g018920 32.743 113 69 4 1 107 1 112 3.04e-13 68.2
MS.gene48720.t1 MTR_6g005450 33.628 113 68 4 1 107 1 112 1.32e-12 66.2
MS.gene48720.t1 MTR_4g084780 38.739 111 52 2 74 168 1 111 1.30e-11 60.8
MS.gene48720.t1 MTR_6g005440 35.398 113 66 3 1 107 1 112 2.33e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48720.t1 AT5G48670 55.844 154 67 1 1 154 1 153 7.38e-47 157
MS.gene48720.t1 AT1G65330 42.246 187 94 3 3 176 2 187 4.56e-41 141
MS.gene48720.t1 AT1G65300 41.436 181 97 2 3 175 2 181 8.55e-41 140
MS.gene48720.t1 AT5G26630 44.503 191 95 2 1 181 1 190 7.84e-40 136
MS.gene48720.t1 AT5G26650 36.667 210 114 4 5 204 3 203 7.82e-36 130
MS.gene48720.t1 AT5G27960 40.476 168 89 3 5 162 3 169 3.09e-35 127
MS.gene48720.t1 AT1G31630 36.905 168 95 3 3 161 2 167 1.47e-34 126
MS.gene48720.t1 AT1G31640 39.735 151 89 2 3 153 2 150 7.00e-33 124
MS.gene48720.t1 AT3G05860 37.975 158 97 1 1 158 1 157 3.21e-31 114
MS.gene48720.t1 AT3G05860 37.975 158 97 1 1 158 1 157 5.05e-31 114
MS.gene48720.t1 AT3G05860 37.975 158 97 1 1 158 1 157 5.13e-31 115
MS.gene48720.t1 AT5G27810 55.914 93 41 0 29 121 1 93 8.82e-31 110
MS.gene48720.t1 AT2G28700 37.423 163 97 2 1 161 1 160 1.60e-29 112
MS.gene48720.t1 AT1G22590 32.099 162 106 1 1 162 1 158 2.04e-26 100
MS.gene48720.t1 AT5G26580 34.416 154 86 2 1 154 1 139 1.17e-23 97.1
MS.gene48720.t1 AT5G06500 35.032 157 100 2 1 157 1 155 1.27e-21 89.7
MS.gene48720.t1 AT2G40210 37.209 129 80 1 1 129 1 128 1.74e-16 77.4
MS.gene48720.t1 AT5G58890 37.795 127 68 5 6 123 6 130 2.30e-15 73.6
MS.gene48720.t1 AT5G55690 42.308 78 38 2 1 71 1 78 1.43e-12 65.9
MS.gene48720.t1 AT5G55690 42.308 78 38 2 1 71 1 78 1.43e-12 65.9
MS.gene48720.t1 AT5G27090 34.444 90 58 1 7 95 23 112 7.11e-11 59.7
MS.gene48720.t1 AT5G27944 39.726 73 43 1 24 95 41 113 9.60e-11 60.1

Find 60 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAACAAAGAAAAGAAAAT+AGG 0.231520 4.4:-72108398 MS.gene48720:CDS
CCCTTTGGTGAGATCAATTA+TGG 0.234667 4.4:-72108081 MS.gene48720:CDS
TACCAATGTCCTTCAGATTA+TGG 0.267347 4.4:+72108271 None:intergenic
ATAATTTCATCATCATTAAT+AGG 0.285530 4.4:+72108159 None:intergenic
GTGAGATCAATTATGGAGTT+TGG 0.309548 4.4:-72108074 MS.gene48720:CDS
AACAAAGGGTCTATTGAAAA+AGG 0.314581 4.4:-72108631 MS.gene48720:CDS
AGCATCTAGGAGTGAGGAAA+TGG 0.344298 4.4:-72108211 MS.gene48720:CDS
ATGAGCCTCAACCTGAGATT+TGG 0.345571 4.4:-72108551 MS.gene48720:CDS
ATCCTTCAAGGTAGTAAATC+AGG 0.372648 4.4:+72108051 None:intergenic
TATGCAAAGTCAATTATTGA+TGG 0.385590 4.4:-72108139 MS.gene48720:CDS
CTAGATGCTGTTGGAGGTGG+TGG 0.417295 4.4:+72108225 None:intergenic
GAAACAAAGAAAAGAAAATA+GGG 0.419680 4.4:-72108397 MS.gene48720:CDS
GGCCTGATTTACTACCTTGA+AGG 0.422090 4.4:-72108053 None:intergenic
GAAGGACATTGGTAGACGTT+TGG 0.439260 4.4:-72108262 MS.gene48720:CDS
AGTGAGGAAATGGGAATTGT+GGG 0.443118 4.4:-72108201 MS.gene48720:CDS
TTGTGGGTCATGGCCATGTT+GGG 0.452922 4.4:-72108185 MS.gene48720:CDS
CACAACCATGGCTAGAAGTA+AGG 0.457305 4.4:-72108709 None:intergenic
CTCATAAGAGCCATACACTA+TGG 0.467809 4.4:+72108569 None:intergenic
ATTGTGGGTCATGGCCATGT+TGG 0.468248 4.4:-72108186 MS.gene48720:CDS
TCTGATGAAGGCCAAATCTC+AGG 0.474702 4.4:+72108540 None:intergenic
CTCCTAGATGCTGTTGGAGG+TGG 0.476689 4.4:+72108222 None:intergenic
TCAATTATTGATGGACTCAA+TGG 0.482426 4.4:-72108130 MS.gene48720:CDS
TTGATTTGAGTTATCTTACT+TGG 0.482489 4.4:-72108302 MS.gene48720:CDS
TCCTCACTCCTAGATGCTGT+TGG 0.486555 4.4:+72108216 None:intergenic
CTTGACACAAAGGATTCTGA+AGG 0.491635 4.4:-72108436 MS.gene48720:CDS
TGCAGCATCATTGACAATGA+AGG 0.502295 4.4:+72108677 None:intergenic
AAGAAAAGAAAATAGGGAGC+AGG 0.506419 4.4:-72108391 MS.gene48720:CDS
TTTGTGTCAAGAAAGTCTCT+TGG 0.512375 4.4:+72108448 None:intergenic
GATGCTGTTGGAGGTGGTGG+TGG 0.513228 4.4:+72108228 None:intergenic
GAGTGAGGAAATGGGAATTG+TGG 0.516317 4.4:-72108202 MS.gene48720:CDS
GGACATTGGTAGACGTTTGG+CGG 0.524285 4.4:-72108259 MS.gene48720:CDS
GAGATTTGGCCTTCATCAGA+GGG 0.524738 4.4:-72108537 MS.gene48720:CDS
TCACTCCTAGATGCTGTTGG+AGG 0.527908 4.4:+72108219 None:intergenic
CACCACCTCCAACAGCATCT+AGG 0.540352 4.4:-72108224 MS.gene48720:CDS
ATGTCTCATTGCCTCAATGA+AGG 0.543320 4.4:-72108354 MS.gene48720:CDS
GCATCTAGGAGTGAGGAAAT+GGG 0.544227 4.4:-72108210 MS.gene48720:CDS
CCATAATTGATCTCACCAAA+GGG 0.558369 4.4:+72108081 None:intergenic
GAAGCCTGTGCCATAGTGTA+TGG 0.560968 4.4:-72108579 MS.gene48720:CDS
GAAGGCCAAATCTCAGGTTG+AGG 0.568672 4.4:+72108546 None:intergenic
GTTGATTGACCATAATCTGA+AGG 0.571124 4.4:-72108280 MS.gene48720:CDS
TTTAACCTTACTTCTAGCCA+TGG 0.572129 4.4:+72108704 None:intergenic
TCCATAATTGATCTCACCAA+AGG 0.580970 4.4:+72108080 None:intergenic
GTGCACGAGACAGTGCCCTT+TGG 0.582311 4.4:-72108096 MS.gene48720:CDS
AAGAGACTTTCTTGACACAA+AGG 0.587821 4.4:-72108446 MS.gene48720:CDS
CATTAATAGGTATCCCAACA+TGG 0.589301 4.4:+72108172 None:intergenic
TGAGATTTGGCCTTCATCAG+AGG 0.593649 4.4:-72108538 MS.gene48720:CDS
GACCATAATCTGAAGGACAT+TGG 0.595565 4.4:-72108273 MS.gene48720:CDS
ACAGGCTTCAATGCCACAGA+GGG 0.601811 4.4:+72108593 None:intergenic
TTGATGGACTCAATGGTGGC+TGG 0.607204 4.4:-72108123 MS.gene48720:CDS
GATTTGGCCTTCATCAGAGG+GGG 0.615187 4.4:-72108535 MS.gene48720:CDS
GAAATGGGAATTGTGGGTCA+TGG 0.616732 4.4:-72108195 MS.gene48720:CDS
TCCAACAGCATCTAGGAGTG+AGG 0.643310 4.4:-72108217 MS.gene48720:CDS
GCACAACTCTACCTTCATTG+AGG 0.651806 4.4:+72108343 None:intergenic
TCATTGTCAATGATGCTGCA+AGG 0.652548 4.4:-72108674 MS.gene48720:CDS
AGAGCCATACACTATGGCAC+AGG 0.656790 4.4:+72108575 None:intergenic
ATTATTGATGGACTCAATGG+TGG 0.666274 4.4:-72108127 MS.gene48720:CDS
AGATTTGGCCTTCATCAGAG+GGG 0.672121 4.4:-72108536 MS.gene48720:CDS
GCTGAACTTAGCACCCTCTG+TGG 0.677042 4.4:-72108606 MS.gene48720:CDS
TGTGCACGACAATATGTCAA+CGG 0.688304 4.4:-72108325 MS.gene48720:CDS
CACAGGCTTCAATGCCACAG+AGG 0.696692 4.4:+72108592 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTTCATCATCATTAAT+AGG + chr4.4:72108602-72108621 None:intergenic 15.0%
!! AACATACAAAAAAAGAACAA+AGG - chr4.4:72108112-72108131 MS.gene48720:CDS 20.0%
!! ACATACAAAAAAAGAACAAA+GGG - chr4.4:72108113-72108132 MS.gene48720:CDS 20.0%
!! AGAAACAAAGAAAAGAAAAT+AGG - chr4.4:72108360-72108379 MS.gene48720:CDS 20.0%
!! GAAACAAAGAAAAGAAAATA+GGG - chr4.4:72108361-72108380 MS.gene48720:CDS 20.0%
! TATGCAAAGTCAATTATTGA+TGG - chr4.4:72108619-72108638 MS.gene48720:CDS 25.0%
!! TTGATTTGAGTTATCTTACT+TGG - chr4.4:72108456-72108475 MS.gene48720:CDS 25.0%
AACAAAGGGTCTATTGAAAA+AGG - chr4.4:72108127-72108146 MS.gene48720:CDS 30.0%
!! TCAATTATTGATGGACTCAA+TGG - chr4.4:72108628-72108647 MS.gene48720:CDS 30.0%
AAGAAAAGAAAATAGGGAGC+AGG - chr4.4:72108367-72108386 MS.gene48720:CDS 35.0%
AAGAGACTTTCTTGACACAA+AGG - chr4.4:72108312-72108331 MS.gene48720:CDS 35.0%
CATTAATAGGTATCCCAACA+TGG + chr4.4:72108589-72108608 None:intergenic 35.0%
CCATAATTGATCTCACCAAA+GGG + chr4.4:72108680-72108699 None:intergenic 35.0%
GTGAGATCAATTATGGAGTT+TGG - chr4.4:72108684-72108703 MS.gene48720:CDS 35.0%
GTTGATTGACCATAATCTGA+AGG - chr4.4:72108478-72108497 MS.gene48720:CDS 35.0%
TACCAATGTCCTTCAGATTA+TGG + chr4.4:72108490-72108509 None:intergenic 35.0%
TCCATAATTGATCTCACCAA+AGG + chr4.4:72108681-72108700 None:intergenic 35.0%
TTTGTGTCAAGAAAGTCTCT+TGG + chr4.4:72108313-72108332 None:intergenic 35.0%
!! ATTATTGATGGACTCAATGG+TGG - chr4.4:72108631-72108650 MS.gene48720:CDS 35.0%
!! TGAATTTTGAGAGCACAGAT+TGG + chr4.4:72108250-72108269 None:intergenic 35.0%
AGTGAGGAAATGGGAATTGT+GGG - chr4.4:72108557-72108576 MS.gene48720:CDS 40.0%
ATGTCTCATTGCCTCAATGA+AGG - chr4.4:72108404-72108423 MS.gene48720:CDS 40.0%
CCCTTTGGTGAGATCAATTA+TGG - chr4.4:72108677-72108696 MS.gene48720:CDS 40.0%
CTCATAAGAGCCATACACTA+TGG + chr4.4:72108192-72108211 None:intergenic 40.0%
CTTGACACAAAGGATTCTGA+AGG - chr4.4:72108322-72108341 MS.gene48720:CDS 40.0%
GACCATAATCTGAAGGACAT+TGG - chr4.4:72108485-72108504 MS.gene48720:CDS 40.0%
TCATTGTCAATGATGCTGCA+AGG - chr4.4:72108084-72108103 MS.gene48720:CDS 40.0%
TGCAGCATCATTGACAATGA+AGG + chr4.4:72108084-72108103 None:intergenic 40.0%
TGTGCACGACAATATGTCAA+CGG - chr4.4:72108433-72108452 MS.gene48720:CDS 40.0%
AGCATCTAGGAGTGAGGAAA+TGG - chr4.4:72108547-72108566 MS.gene48720:CDS 45.0%
ATGAGCCTCAACCTGAGATT+TGG - chr4.4:72108207-72108226 MS.gene48720:CDS 45.0%
GAAATGGGAATTGTGGGTCA+TGG - chr4.4:72108563-72108582 MS.gene48720:CDS 45.0%
GAAGGACATTGGTAGACGTT+TGG - chr4.4:72108496-72108515 MS.gene48720:CDS 45.0%
GAGTGAGGAAATGGGAATTG+TGG - chr4.4:72108556-72108575 MS.gene48720:CDS 45.0%
GCACAACTCTACCTTCATTG+AGG + chr4.4:72108418-72108437 None:intergenic 45.0%
GCATCTAGGAGTGAGGAAAT+GGG - chr4.4:72108548-72108567 MS.gene48720:CDS 45.0%
TCTGATGAAGGCCAAATCTC+AGG + chr4.4:72108221-72108240 None:intergenic 45.0%
! AGATTTGGCCTTCATCAGAG+GGG - chr4.4:72108222-72108241 MS.gene48720:CDS 45.0%
! GAGATTTGGCCTTCATCAGA+GGG - chr4.4:72108221-72108240 MS.gene48720:CDS 45.0%
! TGAGATTTGGCCTTCATCAG+AGG - chr4.4:72108220-72108239 MS.gene48720:CDS 45.0%
ACAGGCTTCAATGCCACAGA+GGG + chr4.4:72108168-72108187 None:intergenic 50.0%
AGAGCCATACACTATGGCAC+AGG + chr4.4:72108186-72108205 None:intergenic 50.0%
ATTGTGGGTCATGGCCATGT+TGG - chr4.4:72108572-72108591 MS.gene48720:CDS 50.0%
GAAGCCTGTGCCATAGTGTA+TGG - chr4.4:72108179-72108198 MS.gene48720:CDS 50.0%
GGACATTGGTAGACGTTTGG+CGG - chr4.4:72108499-72108518 MS.gene48720:CDS 50.0%
TCACTCCTAGATGCTGTTGG+AGG + chr4.4:72108542-72108561 None:intergenic 50.0%
TCCAACAGCATCTAGGAGTG+AGG - chr4.4:72108541-72108560 MS.gene48720:CDS 50.0%
TCCTCACTCCTAGATGCTGT+TGG + chr4.4:72108545-72108564 None:intergenic 50.0%
TTGTGGGTCATGGCCATGTT+GGG - chr4.4:72108573-72108592 MS.gene48720:CDS 50.0%
! GAAGGCCAAATCTCAGGTTG+AGG + chr4.4:72108215-72108234 None:intergenic 50.0%
! GATTTGGCCTTCATCAGAGG+GGG - chr4.4:72108223-72108242 MS.gene48720:CDS 50.0%
!! TTGATGGACTCAATGGTGGC+TGG - chr4.4:72108635-72108654 MS.gene48720:CDS 50.0%
CACAGGCTTCAATGCCACAG+AGG + chr4.4:72108169-72108188 None:intergenic 55.0%
CACCACCTCCAACAGCATCT+AGG - chr4.4:72108534-72108553 MS.gene48720:CDS 55.0%
CTAGATGCTGTTGGAGGTGG+TGG + chr4.4:72108536-72108555 None:intergenic 55.0%
CTCCTAGATGCTGTTGGAGG+TGG + chr4.4:72108539-72108558 None:intergenic 55.0%
GATTGGACCCCCTCTGATGA+AGG + chr4.4:72108233-72108252 None:intergenic 55.0%
GCTGAACTTAGCACCCTCTG+TGG - chr4.4:72108152-72108171 MS.gene48720:CDS 55.0%
GTGCACGAGACAGTGCCCTT+TGG - chr4.4:72108662-72108681 MS.gene48720:CDS 60.0%
! GATGCTGTTGGAGGTGGTGG+TGG + chr4.4:72108533-72108552 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 72108056 72108724 72108056 ID=MS.gene48720
chr4.4 mRNA 72108056 72108724 72108056 ID=MS.gene48720.t1;Parent=MS.gene48720
chr4.4 exon 72108056 72108724 72108056 ID=MS.gene48720.t1.exon1;Parent=MS.gene48720.t1
chr4.4 CDS 72108056 72108724 72108056 ID=cds.MS.gene48720.t1;Parent=MS.gene48720.t1
Gene Sequence

>MS.gene48720

ATGGCTAGAAGTAAGGTTAAACTCGCCTTCATTGTCAATGATGCTGCAAGGAAAGCAACATACAAAAAAAGAACAAAGGGTCTATTGAAAAAGGTTGCTGAACTTAGCACCCTCTGTGGCATTGAAGCCTGTGCCATAGTGTATGGCTCTTATGAGCCTCAACCTGAGATTTGGCCTTCATCAGAGGGGGTCCAATCTGTGCTCTCAAAATTCATGACGATGCACGAGTTTCAAAAGTGCAAGAAAATGCTGAACCAAGAGACTTTCTTGACACAAAGGATTCTGAAGGCTGAAGAGAAATTGAAGAAACAAAGAAAAGAAAATAGGGAGCAGGAGATGACAATACTCATGTCTCATTGCCTCAATGAAGGTAGAGTTGTGCACGACAATATGTCAACGGTTGATTTGAGTTATCTTACTTGGTTGATTGACCATAATCTGAAGGACATTGGTAGACGTTTGGCGGCAACAGTGCCACCACCACCTCCAACAGCATCTAGGAGTGAGGAAATGGGAATTGTGGGTCATGGCCATGTTGGGATACCTATTAATGATGATGAAATTATGCAAAGTCAATTATTGATGGACTCAATGGTGGCTGGTAATGTGCACGAGACAGTGCCCTTTGGTGAGATCAATTATGGAGTTTGGCCTGATTTACTACCTTGA
Protein sequence

>MS.gene48720.t1

MARSKVKLAFIVNDAARKATYKKRTKGLLKKVAELSTLCGIEACAIVYGSYEPQPEIWPSSEGVQSVLSKFMTMHEFQKCKKMLNQETFLTQRILKAEEKLKKQRKENREQEMTILMSHCLNEGRVVHDNMSTVDLSYLTWLIDHNLKDIGRRLAATVPPPPPTASRSEEMGIVGHGHVGIPINDDEIMQSQLLMDSMVAGNVHETVPFGEINYGVWPDLLP