AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49054

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49054.t1 MTR_6g051425 28.477 302 190 7 65 344 19 316 1.79e-32 129
MS.gene49054.t1 MTR_5g015480 50.526 95 46 1 69 162 62 156 2.62e-23 100
MS.gene49054.t1 MTR_0558s0020 24.214 318 190 7 16 305 1 295 1.19e-22 99.8
MS.gene49054.t1 MTR_0402s0040 31.282 195 130 3 54 247 17 208 1.89e-21 99.4
MS.gene49054.t1 MTR_6g017200 39.535 129 73 2 31 158 30 154 3.96e-21 91.3
MS.gene49054.t1 MTR_2g070590 41.818 110 63 1 54 162 8 117 1.25e-20 88.6
MS.gene49054.t1 MTR_4g067290 35.669 157 89 5 27 180 27 174 1.47e-19 89.4
MS.gene49054.t1 MTR_4g067290 35.099 151 89 4 27 177 27 168 2.20e-19 88.6
MS.gene49054.t1 MTR_4g067290 35.669 157 89 5 27 180 47 194 2.24e-19 89.4
MS.gene49054.t1 MTR_1g057920 45.455 88 47 1 65 151 36 123 3.25e-19 84.7
MS.gene49054.t1 MTR_4g067290 35.099 151 89 4 27 177 47 188 3.36e-19 88.6
MS.gene49054.t1 MTR_4g067290 35.099 151 89 4 27 177 45 186 3.62e-19 88.6
MS.gene49054.t1 MTR_4g067290 35.811 148 83 4 43 180 89 234 4.25e-19 89.4
MS.gene49054.t1 MTR_8g446020 25.926 189 127 2 123 305 74 255 3.07e-18 85.9
MS.gene49054.t1 MTR_5g039760 29.082 196 111 3 65 259 12 180 8.68e-18 82.8
MS.gene49054.t1 MTR_2g098920 38.776 98 59 1 65 162 82 178 1.27e-16 81.3
MS.gene49054.t1 MTR_6g090505 23.394 218 140 3 108 305 20 230 1.27e-15 80.1
MS.gene49054.t1 MTR_1g070355 26.250 240 159 7 69 300 80 309 2.43e-15 80.5
MS.gene49054.t1 MTR_7g113010 38.136 118 58 2 57 173 451 554 1.50e-14 78.2
MS.gene49054.t1 MTR_7g061980 37.500 112 67 3 65 175 12 121 2.31e-14 70.9
MS.gene49054.t1 MTR_1g083640 21.519 237 147 5 68 276 56 281 2.28e-12 68.9
MS.gene49054.t1 MTR_3g006370 29.870 154 93 2 66 204 53 206 2.96e-12 67.8
MS.gene49054.t1 MTR_0137s0040 54.167 48 22 0 69 116 42 89 3.64e-12 64.3
MS.gene49054.t1 MTR_2g098880 36.111 108 61 2 65 167 63 167 5.92e-12 67.4
MS.gene49054.t1 MTR_2g098880 36.111 108 61 2 65 167 75 179 7.95e-12 67.0
MS.gene49054.t1 MTR_4g102720 37.931 87 52 2 65 151 9 93 2.12e-11 65.1
MS.gene49054.t1 MTR_1g086270 32.500 80 54 0 360 439 1 80 2.73e-11 61.6
MS.gene49054.t1 MTR_7g446520 26.407 231 144 7 69 295 94 302 8.12e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49054.t1 AT4G38180 30.970 691 453 6 13 682 6 693 3.44e-104 341
MS.gene49054.t1 AT4G15090 26.984 819 532 14 66 877 54 813 1.53e-91 308
MS.gene49054.t1 AT4G15090 26.984 819 532 14 66 877 54 813 1.53e-91 308
MS.gene49054.t1 AT4G15090 26.984 819 532 14 66 877 54 813 1.53e-91 308
MS.gene49054.t1 AT4G15090 26.984 819 532 14 66 877 54 813 1.53e-91 308
MS.gene49054.t1 AT4G15090 26.984 819 532 14 66 877 54 813 1.53e-91 308
MS.gene49054.t1 AT3G06250 30.819 623 373 10 66 685 193 760 2.40e-90 303
MS.gene49054.t1 AT3G06250 35.354 99 51 1 65 163 30 115 6.59e-12 70.1
MS.gene49054.t1 AT3G06250 30.819 623 373 10 66 685 193 760 2.40e-90 303
MS.gene49054.t1 AT3G06250 35.354 99 51 1 65 163 30 115 6.59e-12 70.1
MS.gene49054.t1 AT3G06250 30.819 623 373 10 66 685 193 760 2.40e-90 303
MS.gene49054.t1 AT3G06250 35.354 99 51 1 65 163 30 115 6.59e-12 70.1
MS.gene49054.t1 AT5G18960 30.159 630 371 13 66 685 214 784 1.05e-85 291
MS.gene49054.t1 AT5G18960 30.464 151 84 3 13 163 1 130 2.08e-12 71.6
MS.gene49054.t1 AT5G18960 30.159 630 371 13 66 685 214 784 1.05e-85 291
MS.gene49054.t1 AT5G18960 30.464 151 84 3 13 163 1 130 2.08e-12 71.6
MS.gene49054.t1 AT3G22170 26.491 721 457 8 2 698 16 687 1.40e-82 283
MS.gene49054.t1 AT3G22170 26.491 721 457 8 2 698 16 687 1.40e-82 283
MS.gene49054.t1 AT2G27110 31.100 627 392 13 65 689 51 639 1.05e-81 281
MS.gene49054.t1 AT2G27110 31.100 627 392 13 65 689 51 639 1.05e-81 281
MS.gene49054.t1 AT1G76320 29.431 615 410 7 69 680 3 596 1.84e-80 275
MS.gene49054.t1 AT1G76320 29.431 615 410 7 69 680 3 596 1.93e-80 275
MS.gene49054.t1 AT1G76320 29.431 615 410 7 69 680 3 596 1.93e-80 275
MS.gene49054.t1 AT1G76320 29.431 615 410 7 69 680 3 596 2.77e-80 273
MS.gene49054.t1 AT2G32250 27.752 645 407 10 66 704 41 632 8.98e-78 270
MS.gene49054.t1 AT2G32250 27.752 645 407 10 66 704 41 632 8.98e-78 270
MS.gene49054.t1 AT2G32250 27.752 645 407 10 66 704 41 632 1.03e-77 270
MS.gene49054.t1 AT2G32250 27.752 645 407 10 66 704 41 632 1.03e-77 270
MS.gene49054.t1 AT2G32250 27.752 645 407 10 66 704 41 632 1.03e-77 270
MS.gene49054.t1 AT2G32250 27.752 645 407 10 66 704 41 632 1.04e-77 268
MS.gene49054.t1 AT2G32250 27.726 642 405 10 69 704 3 591 1.83e-77 268
MS.gene49054.t1 AT2G32250 27.726 642 405 10 69 704 3 591 2.22e-77 268
MS.gene49054.t1 AT2G27110 32.591 494 313 8 197 689 20 494 8.04e-75 259
MS.gene49054.t1 AT4G19990 28.963 656 406 11 66 703 24 637 1.21e-72 254
MS.gene49054.t1 AT4G19990 28.963 656 406 11 66 703 24 637 1.21e-72 254
MS.gene49054.t1 AT4G19990 28.963 656 406 11 66 703 24 637 1.28e-72 254
MS.gene49054.t1 AT4G19990 28.963 656 406 11 66 703 38 651 1.31e-72 254
MS.gene49054.t1 AT4G19990 28.963 656 406 11 66 703 24 637 1.45e-72 254
MS.gene49054.t1 AT4G19990 28.963 656 406 11 66 703 24 637 1.45e-72 254
MS.gene49054.t1 AT4G38170 28.455 492 322 6 230 719 10 473 4.44e-72 248
MS.gene49054.t1 AT1G52520 29.302 587 354 17 64 623 86 638 7.96e-65 231
MS.gene49054.t1 AT4G19990 28.387 620 384 11 102 703 3 580 9.69e-65 231
MS.gene49054.t1 AT1G80010 27.227 595 389 15 66 635 98 673 2.03e-59 216
MS.gene49054.t1 AT1G10240 24.610 577 402 12 60 620 45 604 4.07e-47 179
MS.gene49054.t1 AT1G10240 24.610 577 402 12 60 620 45 604 4.07e-47 179
MS.gene49054.t1 AT5G28530 24.262 610 376 15 60 622 50 620 2.10e-42 166
MS.gene49054.t1 AT5G28530 24.059 611 372 17 60 622 50 616 4.25e-41 161
MS.gene49054.t1 AT5G28530 24.059 611 372 17 60 622 50 616 4.25e-41 161
MS.gene49054.t1 AT5G28530 22.892 332 209 6 312 622 252 557 1.22e-21 100
MS.gene49054.t1 AT5G28530 22.523 333 205 8 312 622 252 553 2.41e-20 96.7
MS.gene49054.t1 AT3G59470 40.816 98 57 1 65 162 72 168 2.10e-18 86.3
MS.gene49054.t1 AT3G59470 40.816 98 57 1 65 162 72 168 2.10e-18 86.3
MS.gene49054.t1 AT3G59470 40.816 98 57 1 65 162 84 180 2.51e-18 86.3
MS.gene49054.t1 AT3G07500 42.593 108 61 1 65 172 35 141 2.87e-18 85.1
MS.gene49054.t1 AT2G43280 29.221 154 94 3 66 204 25 178 7.18e-14 72.0
MS.gene49054.t1 AT4G12850 32.653 98 63 1 65 162 13 107 4.64e-13 67.8
MS.gene49054.t1 AT4G12850 32.323 99 64 1 65 163 13 108 7.82e-12 64.3
MS.gene49054.t1 AT4G12850 32.653 98 63 1 65 162 13 107 1.62e-11 64.7

Find 174 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTCGGACTGCAACAATTT+TGG 0.148412 1.3:+69829023 None:intergenic
GGTTGACTCATATCATAATT+TGG 0.153971 1.3:-69828552 MS.gene49054:CDS
AGGATTCTGAATGAGGATTT+TGG 0.176822 1.3:+69826114 None:intergenic
CAGCTTGCTACAACCATCTT+AGG 0.224643 1.3:+69827201 None:intergenic
CATGTATACCGGTCTGTTTC+AGG 0.224714 1.3:+69828011 None:intergenic
CGGACTCATGGTATGTGTTT+TGG 0.233173 1.3:+69826297 None:intergenic
AGACCATCGAATCATTTAAT+TGG 0.243258 1.3:-69828133 MS.gene49054:CDS
AGTATGACGAATACAAAATT+AGG 0.255620 1.3:-69828506 MS.gene49054:CDS
ACTTAAAGCTGAACTAGATT+TGG 0.264524 1.3:+69828526 None:intergenic
GATTATGTTGTGCTTCAAAA+TGG 0.267872 1.3:+69828654 None:intergenic
GTGGTAGATTACGACCATTT+TGG 0.280325 1.3:-69828278 MS.gene49054:CDS
TTAGAAAAGATTGGATTAAT+AGG 0.287407 1.3:-69828847 MS.gene49054:CDS
ACTTTCATTGGCGCAATGTC+TGG 0.289475 1.3:-69828101 MS.gene49054:CDS
CTTACCTGCAAAGGGAGATT+TGG 0.310345 1.3:+69826775 None:intergenic
CCTCCACTATCCTTTCAAAA+TGG 0.316329 1.3:+69827661 None:intergenic
TTCTGTGAGGGAAAGAGATA+TGG 0.326936 1.3:-69828432 MS.gene49054:CDS
AGTAAATTCTTCCTCGTCCT+TGG 0.329251 1.3:+69827890 None:intergenic
AAGATAATTGTCATAATTCA+TGG 0.332546 1.3:+69828457 None:intergenic
ACTTGGAACATCCAATAGTT+TGG 0.334138 1.3:-69829059 MS.gene49054:CDS
GTAAATTCTTCCTCGTCCTT+GGG 0.340043 1.3:+69827891 None:intergenic
CACATTCAAGTCCAAACTAT+TGG 0.341769 1.3:+69829048 None:intergenic
CATGCATTGAACCACTTCAT+TGG 0.347987 1.3:+69826524 None:intergenic
CCGAACGTTCTTCACGCTAA+TGG 0.356305 1.3:-69829007 MS.gene49054:CDS
AGCATCTTGTTCTGTGATAA+TGG 0.360975 1.3:+69828064 None:intergenic
AACAACATGTTAGAATTGAT+TGG 0.361206 1.3:+69828617 None:intergenic
TTTCTGGGCTGATGACAATA+TGG 0.363592 1.3:-69828300 MS.gene49054:CDS
GTTGACTCATATCATAATTT+GGG 0.366463 1.3:-69828551 MS.gene49054:CDS
TACATGGTATGGTGGTTTCT+AGG 0.366745 1.3:-69828817 MS.gene49054:CDS
TCAACCAAATCTCCCTTTGC+AGG 0.367806 1.3:-69826779 MS.gene49054:intron
CTTAAAGCTGAACTAGATTT+GGG 0.370982 1.3:+69828527 None:intergenic
GGCTTTGTCAACACAGTGTT+CGG 0.372860 1.3:-69828947 MS.gene49054:CDS
AATCCTTTGCAAATGATCTA+AGG 0.380215 1.3:-69827929 MS.gene49054:CDS
GGTTCAATGTTAGAAAAGAT+TGG 0.384400 1.3:-69828856 MS.gene49054:CDS
AGGAGAAGCTGGACGTTTGA+TGG 0.385911 1.3:-69828405 MS.gene49054:CDS
CCACAGACTTGTCAAGTTAT+CGG 0.386090 1.3:-69827163 MS.gene49054:CDS
GATGTCAAAGGCTTTCATTT+AGG 0.388977 1.3:-69827750 MS.gene49054:CDS
GGGATGTCTTCCAAAGCTAA+TGG 0.397500 1.3:-69827598 MS.gene49054:CDS
TGAAATATTTCAAATGCTCT+TGG 0.400052 1.3:+69827534 None:intergenic
TTTGGTTGAGATGAAACATA+TGG 0.404147 1.3:+69826793 None:intergenic
GGAAGAATTTACTCATGCTT+GGG 0.404564 1.3:-69827880 MS.gene49054:CDS
AGAAGTTTGGCGGTTGGAAC+AGG 0.408816 1.3:+69826457 None:intergenic
TGTTCTGTGATAATGGCCTT+AGG 0.409137 1.3:+69828071 None:intergenic
TGTTGGTTAGAATAGAAGTT+TGG 0.411083 1.3:+69826444 None:intergenic
GAGAAATGGGCTGTCTGCTT+TGG 0.411646 1.3:-69827792 MS.gene49054:CDS
AACAAACAACATCCCCAAAA+TGG 0.411804 1.3:+69828264 None:intergenic
GAGTATAAAGTGAATACATT+TGG 0.412707 1.3:-69827447 MS.gene49054:CDS
TGAGATGGATGTATAGAGAA+AGG 0.414378 1.3:-69827815 MS.gene49054:CDS
TGGCTGTTTCGAACTTTCAT+TGG 0.414516 1.3:-69828113 MS.gene49054:CDS
GTATAGAGAAAGGGAGAAAT+GGG 0.415013 1.3:-69827805 MS.gene49054:CDS
TGTATAGAGAAAGGGAGAAA+TGG 0.415562 1.3:-69827806 MS.gene49054:CDS
TGTATTATCTGAAGAATTGA+AGG 0.418492 1.3:+69827401 None:intergenic
ATCTCAACCATTCATTTCGC+TGG 0.423879 1.3:+69827832 None:intergenic
ACCACATTACCCATTAGCTT+TGG 0.425279 1.3:+69827588 None:intergenic
AACATTGAACCCCATCAATC+TGG 0.425557 1.3:+69828868 None:intergenic
ACAACATGTTAGAATTGATT+GGG 0.429453 1.3:+69828618 None:intergenic
AGTCAGAACTTCGGTATCTA+TGG 0.430265 1.3:+69826075 None:intergenic
TCTTAAACATCTCAGCCATT+TGG 0.431357 1.3:-69827964 MS.gene49054:CDS
ATTCGTCATCTTTAACCAAA+TGG 0.433155 1.3:+69827949 None:intergenic
TGGACATGCGACTGAGGATC+AGG 0.436653 1.3:-69826968 MS.gene49054:CDS
GGAGACGAGAACTGGATGTT+TGG 0.438770 1.3:-69828723 MS.gene49054:CDS
CATCGGAAGGAGACGAGAAC+TGG 0.445746 1.3:-69828731 MS.gene49054:CDS
TAACAAGTATGCCAGATTGA+TGG 0.451658 1.3:-69828879 MS.gene49054:CDS
AATGGCTCTCAAACTCAATC+TGG 0.454597 1.3:-69828989 MS.gene49054:CDS
ATAATCAAACTGATTCAGAT+AGG 0.455395 1.3:-69826873 MS.gene49054:CDS
AGGAAGAATTTACTCATGCT+TGG 0.458452 1.3:-69827881 MS.gene49054:CDS
AACAAGTATGCCAGATTGAT+GGG 0.458593 1.3:-69828878 MS.gene49054:CDS
TGATGATCTAACACAAGATC+AGG 0.463119 1.3:+69826492 None:intergenic
TCCTCAGTCGCATGTCCATT+CGG 0.464228 1.3:+69826973 None:intergenic
GTCAACACAGTGTTCGGAAT+CGG 0.464277 1.3:-69828941 MS.gene49054:CDS
GGATGTCTTCCAAAGCTAAT+GGG 0.465360 1.3:-69827597 MS.gene49054:CDS
AGAAGCTAGTGATGAAATGA+AGG 0.466016 1.3:-69827619 MS.gene49054:CDS
ATCTACATGAATACTATTTG+AGG 0.467176 1.3:+69827026 None:intergenic
ATGCAAGGTTGGGTAATGTC+AGG 0.468937 1.3:-69827254 MS.gene49054:CDS
GAGATGGATGTATAGAGAAA+GGG 0.473743 1.3:-69827814 MS.gene49054:CDS
TGTTGCATCTGATTATTGCT+TGG 0.479286 1.3:+69826272 None:intergenic
TGATAAAAGCTTACCTGCAA+AGG 0.481590 1.3:+69826766 None:intergenic
CAGACCGGTATACATGTGTT+TGG 0.482825 1.3:-69828004 MS.gene49054:CDS
CAGACAACAGTATTTGAAGA+AGG 0.483624 1.3:+69826835 None:intergenic
AGCTTGCTACAACCATCTTA+GGG 0.484144 1.3:+69827202 None:intergenic
GAGGCAACTTGACGAAGTTA+TGG 0.484472 1.3:-69827100 MS.gene49054:CDS
AGGGAAAGAGATATGGGTAA+AGG 0.486429 1.3:-69828425 MS.gene49054:CDS
AATTGAACTACATCAAGGTC+AGG 0.492101 1.3:+69827690 None:intergenic
AATTCAAGATAACCCTAAGA+TGG 0.492105 1.3:-69827214 MS.gene49054:CDS
CTCGATATATCTTGAAGAGA+TGG 0.495398 1.3:-69827284 MS.gene49054:CDS
AATTGACAGGACCGCTACAC+TGG 0.496426 1.3:-69826920 MS.gene49054:CDS
AAGCAATAATCAGATGCAAC+AGG 0.498238 1.3:-69826270 MS.gene49054:intron
CTTTCATTGGCGCAATGTCT+GGG 0.499304 1.3:-69828100 MS.gene49054:CDS
ATGGTCACACAGCAAAATAC+AGG 0.502031 1.3:+69826094 None:intergenic
TCTGGTAATGCATCAGCAAA+TGG 0.509074 1.3:-69828971 MS.gene49054:CDS
TAGGAGTAAGGTACATGGTA+TGG 0.509545 1.3:-69828828 MS.gene49054:CDS
ACGATATCCCTCTCTGGAGC+AGG 0.509622 1.3:+69828787 None:intergenic
GATGGTGATCATAACAACTT+AGG 0.510043 1.3:-69829112 MS.gene49054:CDS
GATAAAAGCTTACCTGCAAA+GGG 0.510969 1.3:+69826767 None:intergenic
CCATTAGCGTGAAGAACGTT+CGG 0.511332 1.3:+69829007 None:intergenic
ATTATGTTGTGCTTCAAAAT+GGG 0.511977 1.3:+69828655 None:intergenic
GTCATCAACATCGAGCTGTA+TGG 0.515154 1.3:+69828337 None:intergenic
TGAGATGAAACATATGGAGA+TGG 0.517004 1.3:+69826799 None:intergenic
GAGATGAAACATATGGAGAT+GGG 0.517572 1.3:+69826800 None:intergenic
ATGGACAAAAGATGCAAGGT+TGG 0.517774 1.3:-69827265 MS.gene49054:CDS
TAGAATAGAAGTTTGGCGGT+TGG 0.518493 1.3:+69826451 None:intergenic
AAAATACAGGATTCTGAATG+AGG 0.519697 1.3:+69826107 None:intergenic
ATCATTACTCGTCAACAAGA+TGG 0.520020 1.3:-69828692 MS.gene49054:CDS
GAAAATGTTGATGTTGGACT+TGG 0.524439 1.3:-69829076 MS.gene49054:CDS
TTTCCTACGATATCCCTCTC+TGG 0.525239 1.3:+69828781 None:intergenic
TATTGCTTGGCACGGACTCA+TGG 0.526252 1.3:+69826285 None:intergenic
GGAGAAGCTGGACGTTTGAT+GGG 0.526650 1.3:-69828404 MS.gene49054:CDS
TGGTTAGAATAGAAGTTTGG+CGG 0.528921 1.3:+69826447 None:intergenic
CATGTAGAAAATGTTGATGT+TGG 0.531700 1.3:-69829082 MS.gene49054:CDS
GATATGGGTAAAGGAGAAGC+TGG 0.535036 1.3:-69828416 MS.gene49054:CDS
AGATTGGATTAATAGGAGTA+AGG 0.541743 1.3:-69828840 MS.gene49054:CDS
GATTATAATTCACCGTTGGT+TGG 0.543940 1.3:+69826890 None:intergenic
CAATAGTTTGGACTTGAATG+TGG 0.544732 1.3:-69829047 MS.gene49054:CDS
TGGACAAAAGATGCAAGGTT+GGG 0.547513 1.3:-69827264 MS.gene49054:CDS
TTGCCTTTCTCAACTATCGC+AGG 0.548639 1.3:-69826412 MS.gene49054:intron
CAGCCAATTAAATGATTCGA+TGG 0.552432 1.3:+69828130 None:intergenic
TTGTGTTGCTCAAGCTGTGG+AGG 0.559431 1.3:-69828573 MS.gene49054:CDS
GTTTGATTATAATTCACCGT+TGG 0.560586 1.3:+69826886 None:intergenic
ATAAACTCCAGCGAAATGAA+TGG 0.563219 1.3:-69827839 MS.gene49054:CDS
GTCAACCAGCACAAAAGAGA+TGG 0.564837 1.3:-69827480 MS.gene49054:CDS
CAATTATCTTCATTCTGTGA+GGG 0.569801 1.3:-69828444 MS.gene49054:CDS
TAAATTCTTCCTCGTCCTTG+GGG 0.571062 1.3:+69827892 None:intergenic
TAAGAAATTGAACTACATCA+AGG 0.574535 1.3:+69827685 None:intergenic
AGAATTTACTCATGCTTGGG+GGG 0.575196 1.3:-69827877 MS.gene49054:CDS
AAAGGCAAGGAGAACTCTGT+TGG 0.577643 1.3:+69826427 None:intergenic
GAAGAATTTACTCATGCTTG+GGG 0.577829 1.3:-69827879 MS.gene49054:CDS
TCTGTGAGGGAAAGAGATAT+GGG 0.578639 1.3:-69828431 MS.gene49054:CDS
CACAGTGTTCGGAATCGGTA+CGG 0.578865 1.3:-69828936 MS.gene49054:CDS
TCCAAAGCTAATGGGTAATG+TGG 0.580465 1.3:-69827589 MS.gene49054:CDS
AATCTTGATATTGAAAAGTG+AGG 0.582977 1.3:-69827064 MS.gene49054:CDS
ACAGGACCGCTACACTGGAT+AGG 0.587540 1.3:-69826915 MS.gene49054:CDS
AAATTTACCTGCTCCAGAGA+GGG 0.588352 1.3:-69828794 MS.gene49054:CDS
AAGAGCTTCTGAATCTGTGG+AGG 0.588474 1.3:-69827139 MS.gene49054:CDS
TACTGTGAAGAAACATCGGA+AGG 0.591189 1.3:-69828744 MS.gene49054:CDS
AGCGAAATGAATGGTTGAGA+TGG 0.591609 1.3:-69827830 MS.gene49054:CDS
CCATTCGGAAAGATCACCGC+AGG 0.593497 1.3:+69826988 None:intergenic
GAGTAAGCGAAGAAATTGAC+AGG 0.593964 1.3:-69826933 MS.gene49054:CDS
ACACCTGCGATAGTTGAGAA+AGG 0.595685 1.3:+69826409 None:intergenic
GGCAAGAGCTTCTGAATCTG+TGG 0.597094 1.3:-69827142 MS.gene49054:CDS
GAGTAAGGTACATGGTATGG+TGG 0.599166 1.3:-69828825 MS.gene49054:CDS
TGCAAGGTTGGGTAATGTCA+GGG 0.602166 1.3:-69827253 MS.gene49054:CDS
TCTGCCAAACACATGTATAC+CGG 0.603544 1.3:+69828000 None:intergenic
CATCTGATTATTGCTTGGCA+CGG 0.603767 1.3:+69826277 None:intergenic
TATATATGACCCCAAGGACG+AGG 0.604602 1.3:-69827901 MS.gene49054:CDS
ACTCCTTAGATCATTTGCAA+AGG 0.605967 1.3:+69827926 None:intergenic
TCCGAATGGACATGCGACTG+AGG 0.607571 1.3:-69826974 MS.gene49054:CDS
ACTGGATAGGTGCCAACCAA+CGG 0.611215 1.3:-69826902 MS.gene49054:CDS
ACAATTATCTTCATTCTGTG+AGG 0.613083 1.3:-69828445 MS.gene49054:CDS
AGATGAAACATATGGAGATG+GGG 0.614637 1.3:+69826801 None:intergenic
TGTATGAAGTTTGAGCATGT+AGG 0.618522 1.3:-69827366 MS.gene49054:CDS
CTCTTCAAGATATATCGAGA+TGG 0.620754 1.3:+69827288 None:intergenic
AAGAATTTACTCATGCTTGG+GGG 0.622092 1.3:-69827878 MS.gene49054:CDS
TCAACAAGATGGAAAATACC+AGG 0.623065 1.3:-69828681 MS.gene49054:CDS
CCTGCGGTGATCTTTCCGAA+TGG 0.627373 1.3:-69826988 MS.gene49054:CDS
GTGCTTCAAAATGGGTAACC+TGG 0.628259 1.3:+69828663 None:intergenic
GATGAAACATATGGAGATGG+GGG 0.631905 1.3:+69826802 None:intergenic
AGAGATGGACAAAAGATGCA+AGG 0.632488 1.3:-69827269 MS.gene49054:CDS
TGCTTCAGAAAGTGAGCCTG+CGG 0.635679 1.3:-69827004 MS.gene49054:CDS
CAACTTGACGAAGTTATGGA+AGG 0.645660 1.3:-69827096 MS.gene49054:CDS
GAAGCTAGTGATGAAATGAA+GGG 0.646836 1.3:-69827618 MS.gene49054:CDS
CCGATAACTTGACAAGTCTG+TGG 0.655057 1.3:+69827163 None:intergenic
GAGTTGTATATATGACCCCA+AGG 0.660099 1.3:-69827907 MS.gene49054:CDS
ATTAATAGGAGTAAGGTACA+TGG 0.662022 1.3:-69828833 MS.gene49054:CDS
TGCGATAGTTGAGAAAGGCA+AGG 0.663759 1.3:+69826414 None:intergenic
AGAGCTTCTGAATCTGTGGA+GGG 0.666121 1.3:-69827138 MS.gene49054:CDS
GAAATTTACCTGCTCCAGAG+AGG 0.668278 1.3:-69828795 MS.gene49054:CDS
CTGGGCTGATGACAATATGG+TGG 0.677851 1.3:-69828297 MS.gene49054:CDS
GGTAATGCATCAGCAAATGG+AGG 0.677979 1.3:-69828968 MS.gene49054:CDS
GTTATGCAGAGTCAGAACTT+CGG 0.680514 1.3:+69826066 None:intergenic
TTGGCACCTATCCAGTGTAG+CGG 0.682153 1.3:+69826909 None:intergenic
ACAAGTATGCCAGATTGATG+GGG 0.683870 1.3:-69828877 MS.gene49054:CDS
AGGGTTATCAGTTTGCTGTG+AGG 0.684882 1.3:-69827119 MS.gene49054:CDS
ATGAAACATATGGAGATGGG+GGG 0.693657 1.3:+69826803 None:intergenic
GCTCCAGAGAGGGATATCGT+AGG 0.703657 1.3:-69828784 MS.gene49054:CDS
AATCATCGAAGCAATCAATG+TGG 0.773569 1.3:-69828039 MS.gene49054:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATAAAAAATAAAGAGAA+CGG - chr1.3:69828586-69828605 MS.gene49054:CDS 15.0%
!! TCATTTATTTGATTGATATA+TGG - chr1.3:69828436-69828455 MS.gene49054:CDS 15.0%
!! AAGATAATTGTCATAATTCA+TGG + chr1.3:69826728-69826747 None:intergenic 20.0%
!! TATAAAAAAAACCTAATGAC+TGG + chr1.3:69828807-69828826 None:intergenic 20.0%
!! TTAGAAAAGATTGGATTAAT+AGG - chr1.3:69826335-69826354 MS.gene49054:intron 20.0%
!!! AATTTCTTAACCATTTTGAA+AGG - chr1.3:69827511-69827530 MS.gene49054:CDS 20.0%
!!! ATATCATGAATATTTTACTC+AGG - chr1.3:69828852-69828871 MS.gene49054:CDS 20.0%
!!! ATTTTCTCATTTGTTTTACA+GGG - chr1.3:69828618-69828637 MS.gene49054:CDS 20.0%
! AACAACATGTTAGAATTGAT+TGG + chr1.3:69826568-69826587 None:intergenic 25.0%
! AATCTTGATATTGAAAAGTG+AGG - chr1.3:69828118-69828137 MS.gene49054:CDS 25.0%
! ACAACATGTTAGAATTGATT+GGG + chr1.3:69826567-69826586 None:intergenic 25.0%
! ACAGGTAAACATTTACTTAA+TGG - chr1.3:69828930-69828949 MS.gene49054:CDS 25.0%
! AGTATGACGAATACAAAATT+AGG - chr1.3:69826676-69826695 MS.gene49054:intron 25.0%
! ATCTACATGAATACTATTTG+AGG + chr1.3:69828159-69828178 None:intergenic 25.0%
! ATTATGTTGTGCTTCAAAAT+GGG + chr1.3:69826530-69826549 None:intergenic 25.0%
! GAGTATAAAGTGAATACATT+TGG - chr1.3:69827735-69827754 MS.gene49054:CDS 25.0%
! TAAGAAATTGAACTACATCA+AGG + chr1.3:69827500-69827519 None:intergenic 25.0%
! TGAAATATTTCAAATGCTCT+TGG + chr1.3:69827651-69827670 None:intergenic 25.0%
! TGTATTATCTGAAGAATTGA+AGG + chr1.3:69827784-69827803 None:intergenic 25.0%
!! ACAAGATAACAAATCTTTTC+TGG - chr1.3:69826866-69826885 MS.gene49054:CDS 25.0%
!! ATAATCAAACTGATTCAGAT+AGG - chr1.3:69828309-69828328 MS.gene49054:CDS 25.0%
!! ATTTTACTGTGAAGAAACAT+CGG - chr1.3:69826434-69826453 MS.gene49054:CDS 25.0%
!! CAAGATAACAAATCTTTTCT+GGG - chr1.3:69826867-69826886 MS.gene49054:CDS 25.0%
!! GTTGACTCATATCATAATTT+GGG - chr1.3:69826631-69826650 MS.gene49054:intron 25.0%
!! TGTTAATTTTTCCAGTCATT+AGG - chr1.3:69828793-69828812 MS.gene49054:CDS 25.0%
!!! AGATTTTTTGTAGATGTCAA+AGG - chr1.3:69827420-69827439 MS.gene49054:CDS 25.0%
!!! GATTTTCTCATTTGTTTTAC+AGG - chr1.3:69828617-69828636 MS.gene49054:CDS 25.0%
AAAATACAGGATTCTGAATG+AGG + chr1.3:69829078-69829097 None:intergenic 30.0%
AATCCTTTGCAAATGATCTA+AGG - chr1.3:69827253-69827272 MS.gene49054:CDS 30.0%
AATTCAAGATAACCCTAAGA+TGG - chr1.3:69827968-69827987 MS.gene49054:CDS 30.0%
ACAATTATCTTCATTCTGTG+AGG - chr1.3:69826737-69826756 MS.gene49054:intron 30.0%
ACACCTAAAAACTGTACAAA+TGG + chr1.3:69826979-69826998 None:intergenic 30.0%
ACTTAAAGCTGAACTAGATT+TGG + chr1.3:69826659-69826678 None:intergenic 30.0%
AGACCATCGAATCATTTAAT+TGG - chr1.3:69827049-69827068 MS.gene49054:CDS 30.0%
AGATTGGATTAATAGGAGTA+AGG - chr1.3:69826342-69826361 MS.gene49054:intron 30.0%
ATTAATAGGAGTAAGGTACA+TGG - chr1.3:69826349-69826368 MS.gene49054:intron 30.0%
ATTCGTCATCTTTAACCAAA+TGG + chr1.3:69827236-69827255 None:intergenic 30.0%
CAATTATCTTCATTCTGTGA+GGG - chr1.3:69826738-69826757 MS.gene49054:intron 30.0%
CATGTAGAAAATGTTGATGT+TGG - chr1.3:69826100-69826119 MS.gene49054:CDS 30.0%
CTTAAAGCTGAACTAGATTT+GGG + chr1.3:69826658-69826677 None:intergenic 30.0%
GATTATGTTGTGCTTCAAAA+TGG + chr1.3:69826531-69826550 None:intergenic 30.0%
GCATATTTGATATAGAGATC+AGG - chr1.3:69828486-69828505 MS.gene49054:CDS 30.0%
GGTTCAATGTTAGAAAAGAT+TGG - chr1.3:69826326-69826345 MS.gene49054:intron 30.0%
GTTTGATTATAATTCACCGT+TGG + chr1.3:69828299-69828318 None:intergenic 30.0%
TGTATGGCATAAAAAAATGC+TGG + chr1.3:69826832-69826851 None:intergenic 30.0%
TTTGGTTGAGATGAAACATA+TGG + chr1.3:69828392-69828411 None:intergenic 30.0%
! GGTTGACTCATATCATAATT+TGG - chr1.3:69826630-69826649 MS.gene49054:intron 30.0%
! GTTTTATGCTCGTTAACTTT+TGG - chr1.3:69828541-69828560 MS.gene49054:CDS 30.0%
! TATCAAGAACTTTTAGTGCA+TGG + chr1.3:69827854-69827873 None:intergenic 30.0%
! TGTTGGTTAGAATAGAAGTT+TGG + chr1.3:69828741-69828760 None:intergenic 30.0%
!! CTTTGTGTATATTTGCTGTA+GGG - chr1.3:69829030-69829049 MS.gene49054:CDS 30.0%
!! TAACCATTTTGAAAGGATAG+TGG - chr1.3:69827518-69827537 MS.gene49054:CDS 30.0%
!! TGGTATGTGTTTTGGATTAT+AGG + chr1.3:69828880-69828899 None:intergenic 30.0%
!!! GTTTGATGGGTTATTTTCAA+AGG - chr1.3:69826791-69826810 MS.gene49054:CDS 30.0%
!!! TTTGTTTTACAGGGCTTATT+TGG - chr1.3:69828627-69828646 MS.gene49054:CDS 30.0%
AACAAACAACATCCCCAAAA+TGG + chr1.3:69826921-69826940 None:intergenic 35.0%
AACAAGTATGCCAGATTGAT+GGG - chr1.3:69826304-69826323 MS.gene49054:CDS 35.0%
AAGAATTTACTCATGCTTGG+GGG - chr1.3:69827304-69827323 MS.gene49054:CDS 35.0%
AAGCAATAATCAGATGCAAC+AGG - chr1.3:69828912-69828931 MS.gene49054:CDS 35.0%
AATCATCGAAGCAATCAATG+TGG - chr1.3:69827143-69827162 MS.gene49054:CDS 35.0%
AATTGAACTACATCAAGGTC+AGG + chr1.3:69827495-69827514 None:intergenic 35.0%
ACTCCTTAGATCATTTGCAA+AGG + chr1.3:69827259-69827278 None:intergenic 35.0%
ACTTGGAACATCCAATAGTT+TGG - chr1.3:69826123-69826142 MS.gene49054:CDS 35.0%
AGAAGCTAGTGATGAAATGA+AGG - chr1.3:69827563-69827582 MS.gene49054:CDS 35.0%
AGATGAAACATATGGAGATG+GGG + chr1.3:69828384-69828403 None:intergenic 35.0%
AGCATCTTGTTCTGTGATAA+TGG + chr1.3:69827121-69827140 None:intergenic 35.0%
AGGAAGAATTTACTCATGCT+TGG - chr1.3:69827301-69827320 MS.gene49054:CDS 35.0%
ATAAACTCCAGCGAAATGAA+TGG - chr1.3:69827343-69827362 MS.gene49054:CDS 35.0%
ATCATTACTCGTCAACAAGA+TGG - chr1.3:69826490-69826509 MS.gene49054:CDS 35.0%
CAATAGTTTGGACTTGAATG+TGG - chr1.3:69826135-69826154 MS.gene49054:intron 35.0%
CACATTCAAGTCCAAACTAT+TGG + chr1.3:69826137-69826156 None:intergenic 35.0%
CAGACAACAGTATTTGAAGA+AGG + chr1.3:69828350-69828369 None:intergenic 35.0%
CAGCCAATTAAATGATTCGA+TGG + chr1.3:69827055-69827074 None:intergenic 35.0%
CTCGATATATCTTGAAGAGA+TGG - chr1.3:69827898-69827917 MS.gene49054:CDS 35.0%
CTCTTCAAGATATATCGAGA+TGG + chr1.3:69827897-69827916 None:intergenic 35.0%
GAAAATGTTGATGTTGGACT+TGG - chr1.3:69826106-69826125 MS.gene49054:CDS 35.0%
GAAGAATTTACTCATGCTTG+GGG - chr1.3:69827303-69827322 MS.gene49054:CDS 35.0%
GAAGCTAGTGATGAAATGAA+GGG - chr1.3:69827564-69827583 MS.gene49054:CDS 35.0%
GAGATGAAACATATGGAGAT+GGG + chr1.3:69828385-69828404 None:intergenic 35.0%
GAGATGGATGTATAGAGAAA+GGG - chr1.3:69827368-69827387 MS.gene49054:CDS 35.0%
GATAAAAGCTTACCTGCAAA+GGG + chr1.3:69828418-69828437 None:intergenic 35.0%
GATGGTGATCATAACAACTT+AGG - chr1.3:69826070-69826089 MS.gene49054:CDS 35.0%
GATGTCAAAGGCTTTCATTT+AGG - chr1.3:69827432-69827451 MS.gene49054:CDS 35.0%
GATTATAATTCACCGTTGGT+TGG + chr1.3:69828295-69828314 None:intergenic 35.0%
GGAAGAATTTACTCATGCTT+GGG - chr1.3:69827302-69827321 MS.gene49054:CDS 35.0%
GTATAGAGAAAGGGAGAAAT+GGG - chr1.3:69827377-69827396 MS.gene49054:CDS 35.0%
TAACAAGTATGCCAGATTGA+TGG - chr1.3:69826303-69826322 MS.gene49054:CDS 35.0%
TCAACAAGATGGAAAATACC+AGG - chr1.3:69826501-69826520 MS.gene49054:CDS 35.0%
TCTTAAACATCTCAGCCATT+TGG - chr1.3:69827218-69827237 MS.gene49054:CDS 35.0%
TGAGATGAAACATATGGAGA+TGG + chr1.3:69828386-69828405 None:intergenic 35.0%
TGAGATGGATGTATAGAGAA+AGG - chr1.3:69827367-69827386 MS.gene49054:CDS 35.0%
TGATAAAAGCTTACCTGCAA+AGG + chr1.3:69828419-69828438 None:intergenic 35.0%
TGATGATCTAACACAAGATC+AGG + chr1.3:69828693-69828712 None:intergenic 35.0%
TGGTTAGAATAGAAGTTTGG+CGG + chr1.3:69828738-69828757 None:intergenic 35.0%
TGTATAGAGAAAGGGAGAAA+TGG - chr1.3:69827376-69827395 MS.gene49054:CDS 35.0%
TGTATGAAGTTTGAGCATGT+AGG - chr1.3:69827816-69827835 MS.gene49054:CDS 35.0%
TGTTGCATCTGATTATTGCT+TGG + chr1.3:69828913-69828932 None:intergenic 35.0%
! AATATCCATCTCTTTTGTGC+TGG + chr1.3:69827710-69827729 None:intergenic 35.0%
! AGGATTCTGAATGAGGATTT+TGG + chr1.3:69829071-69829090 None:intergenic 35.0%
! TGGTAGATTACGACCATTTT+GGG - chr1.3:69826905-69826924 MS.gene49054:CDS 35.0%
!! GCTTTGTGTATATTTGCTGT+AGG - chr1.3:69829029-69829048 MS.gene49054:CDS 35.0%
!! TGTATATTTGCTGTAGGGTA+TGG - chr1.3:69829035-69829054 MS.gene49054:CDS 35.0%
!! TTTTGGGGATGTTGTTTGTT+TGG - chr1.3:69826921-69826940 MS.gene49054:CDS 35.0%
!!! TGGTTTTGAAACCAATGAAG+TGG - chr1.3:69828647-69828666 MS.gene49054:CDS 35.0%
AAATTTACCTGCTCCAGAGA+GGG - chr1.3:69826388-69826407 MS.gene49054:intron 40.0%
AACATTGAACCCCATCAATC+TGG + chr1.3:69826317-69826336 None:intergenic 40.0%
AATGGCTCTCAAACTCAATC+TGG - chr1.3:69826193-69826212 MS.gene49054:intron 40.0%
AATGTCTGGGAAAAAGCCTA+AGG - chr1.3:69827095-69827114 MS.gene49054:CDS 40.0%
ACAAGTATGCCAGATTGATG+GGG - chr1.3:69826305-69826324 MS.gene49054:CDS 40.0%
AGAATTTACTCATGCTTGGG+GGG - chr1.3:69827305-69827324 MS.gene49054:CDS 40.0%
AGAGATGGACAAAAGATGCA+AGG - chr1.3:69827913-69827932 MS.gene49054:CDS 40.0%
AGCGAAATGAATGGTTGAGA+TGG - chr1.3:69827352-69827371 MS.gene49054:CDS 40.0%
AGGGAAAGAGATATGGGTAA+AGG - chr1.3:69826757-69826776 MS.gene49054:intron 40.0%
AGTAAATTCTTCCTCGTCCT+TGG + chr1.3:69827295-69827314 None:intergenic 40.0%
AGTCAGAACTTCGGTATCTA+TGG + chr1.3:69829110-69829129 None:intergenic 40.0%
ATCTCAACCATTCATTTCGC+TGG + chr1.3:69827353-69827372 None:intergenic 40.0%
ATGAAACATATGGAGATGGG+GGG + chr1.3:69828382-69828401 None:intergenic 40.0%
ATGGACAAAAGATGCAAGGT+TGG - chr1.3:69827917-69827936 MS.gene49054:CDS 40.0%
ATGGTCACACAGCAAAATAC+AGG + chr1.3:69829091-69829110 None:intergenic 40.0%
CAACTTGACGAAGTTATGGA+AGG - chr1.3:69828086-69828105 MS.gene49054:CDS 40.0%
CATCTGATTATTGCTTGGCA+CGG + chr1.3:69828908-69828927 None:intergenic 40.0%
CATGCATTGAACCACTTCAT+TGG + chr1.3:69828661-69828680 None:intergenic 40.0%
CCACAGACTTGTCAAGTTAT+CGG - chr1.3:69828019-69828038 MS.gene49054:CDS 40.0%
CCTCCACTATCCTTTCAAAA+TGG + chr1.3:69827524-69827543 None:intergenic 40.0%
CGATAACCCACCAAAAGTTT+GGG + chr1.3:69828979-69828998 None:intergenic 40.0%
GAGTAAGCGAAGAAATTGAC+AGG - chr1.3:69828249-69828268 MS.gene49054:CDS 40.0%
GAGTTGTATATATGACCCCA+AGG - chr1.3:69827275-69827294 MS.gene49054:CDS 40.0%
GATGAAACATATGGAGATGG+GGG + chr1.3:69828383-69828402 None:intergenic 40.0%
GGATGTCTTCCAAAGCTAAT+GGG - chr1.3:69827585-69827604 MS.gene49054:CDS 40.0%
GTAAATTCTTCCTCGTCCTT+GGG + chr1.3:69827294-69827313 None:intergenic 40.0%
TAAATTCTTCCTCGTCCTTG+GGG + chr1.3:69827293-69827312 None:intergenic 40.0%
TACATGGTATGGTGGTTTCT+AGG - chr1.3:69826365-69826384 MS.gene49054:intron 40.0%
TACTGTGAAGAAACATCGGA+AGG - chr1.3:69826438-69826457 MS.gene49054:CDS 40.0%
TAGAATAGAAGTTTGGCGGT+TGG + chr1.3:69828734-69828753 None:intergenic 40.0%
TAGGAGTAAGGTACATGGTA+TGG - chr1.3:69826354-69826373 MS.gene49054:intron 40.0%
TCTGCCAAACACATGTATAC+CGG + chr1.3:69827185-69827204 None:intergenic 40.0%
TCTGTGAGGGAAAGAGATAT+GGG - chr1.3:69826751-69826770 MS.gene49054:intron 40.0%
TGGACAAAAGATGCAAGGTT+GGG - chr1.3:69827918-69827937 MS.gene49054:CDS 40.0%
TTCTGTGAGGGAAAGAGATA+TGG - chr1.3:69826750-69826769 MS.gene49054:intron 40.0%
TTTCTGGGCTGATGACAATA+TGG - chr1.3:69826882-69826901 MS.gene49054:CDS 40.0%
! ACCACATTACCCATTAGCTT+TGG + chr1.3:69827597-69827616 None:intergenic 40.0%
! CGGCCATTTGTACAGTTTTT+AGG - chr1.3:69826973-69826992 MS.gene49054:CDS 40.0%
! GGTAGATTACGACCATTTTG+GGG - chr1.3:69826906-69826925 MS.gene49054:CDS 40.0%
! GTGGTAGATTACGACCATTT+TGG - chr1.3:69826904-69826923 MS.gene49054:CDS 40.0%
! TCCAAAGCTAATGGGTAATG+TGG - chr1.3:69827593-69827612 MS.gene49054:CDS 40.0%
! TCTGGTAATGCATCAGCAAA+TGG - chr1.3:69826211-69826230 MS.gene49054:intron 40.0%
! TGGCTGTTTCGAACTTTCAT+TGG - chr1.3:69827069-69827088 MS.gene49054:CDS 40.0%
!! AGCTTGCTACAACCATCTTA+GGG + chr1.3:69827983-69828002 None:intergenic 40.0%
!! ATTTTGTGTTGCTCAAGCTG+TGG - chr1.3:69826606-69826625 MS.gene49054:intron 40.0%
!! CCATTTTGAAAGGATAGTGG+AGG - chr1.3:69827521-69827540 MS.gene49054:CDS 40.0%
!! TGGTTTTACCTGAAACAGAC+CGG - chr1.3:69827163-69827182 MS.gene49054:CDS 40.0%
!! TGTTCTGTGATAATGGCCTT+AGG + chr1.3:69827114-69827133 None:intergenic 40.0%
!!! CAAACTTTTGGTGGGTTATC+GGG - chr1.3:69828978-69828997 MS.gene49054:CDS 40.0%
!!! CCAAACTTTTGGTGGGTTAT+CGG - chr1.3:69828977-69828996 MS.gene49054:CDS 40.0%
AAAGGCAAGGAGAACTCTGT+TGG + chr1.3:69828758-69828777 None:intergenic 45.0%
AACCCACCAAAAGTTTGGGA+AGG + chr1.3:69828975-69828994 None:intergenic 45.0%
AAGAGCTTCTGAATCTGTGG+AGG - chr1.3:69828043-69828062 MS.gene49054:CDS 45.0%
ACACCTGCGATAGTTGAGAA+AGG + chr1.3:69828776-69828795 None:intergenic 45.0%
ACCCACCAAAAGTTTGGGAA+GGG + chr1.3:69828974-69828993 None:intergenic 45.0%
ACTTTCATTGGCGCAATGTC+TGG - chr1.3:69827081-69827100 MS.gene49054:CDS 45.0%
AGAGCTTCTGAATCTGTGGA+GGG - chr1.3:69828044-69828063 MS.gene49054:CDS 45.0%
ATGCAAGGTTGGGTAATGTC+AGG - chr1.3:69827928-69827947 MS.gene49054:CDS 45.0%
CAGACCGGTATACATGTGTT+TGG - chr1.3:69827178-69827197 MS.gene49054:CDS 45.0%
CATGTATACCGGTCTGTTTC+AGG + chr1.3:69827174-69827193 None:intergenic 45.0%
CCATTAGCGTGAAGAACGTT+CGG + chr1.3:69826178-69826197 None:intergenic 45.0%
CCGATAACCCACCAAAAGTT+TGG + chr1.3:69828980-69828999 None:intergenic 45.0%
CCGATAACTTGACAAGTCTG+TGG + chr1.3:69828022-69828041 None:intergenic 45.0%
CTTACCTGCAAAGGGAGATT+TGG + chr1.3:69828410-69828429 None:intergenic 45.0%
CTTTCATTGGCGCAATGTCT+GGG - chr1.3:69827082-69827101 MS.gene49054:CDS 45.0%
GAAATTTACCTGCTCCAGAG+AGG - chr1.3:69826387-69826406 MS.gene49054:intron 45.0%
GAGGCAACTTGACGAAGTTA+TGG - chr1.3:69828082-69828101 MS.gene49054:CDS 45.0%
GAGTAAGGTACATGGTATGG+TGG - chr1.3:69826357-69826376 MS.gene49054:intron 45.0%
GATATGGGTAAAGGAGAAGC+TGG - chr1.3:69826766-69826785 MS.gene49054:intron 45.0%
GGGATGTCTTCCAAAGCTAA+TGG - chr1.3:69827584-69827603 MS.gene49054:CDS 45.0%
GGTAATGCATCAGCAAATGG+AGG - chr1.3:69826214-69826233 MS.gene49054:intron 45.0%
GTCAACACAGTGTTCGGAAT+CGG - chr1.3:69826241-69826260 MS.gene49054:intron 45.0%
GTCAACCAGCACAAAAGAGA+TGG - chr1.3:69827702-69827721 MS.gene49054:CDS 45.0%
GTCATCAACATCGAGCTGTA+TGG + chr1.3:69826848-69826867 None:intergenic 45.0%
GTGCTTCAAAATGGGTAACC+TGG + chr1.3:69826522-69826541 None:intergenic 45.0%
TATATATGACCCCAAGGACG+AGG - chr1.3:69827281-69827300 MS.gene49054:CDS 45.0%
TCAACCAAATCTCCCTTTGC+AGG - chr1.3:69828403-69828422 MS.gene49054:CDS 45.0%
TGCAAGGTTGGGTAATGTCA+GGG - chr1.3:69827929-69827948 MS.gene49054:CDS 45.0%
TGCGATAGTTGAGAAAGGCA+AGG + chr1.3:69828771-69828790 None:intergenic 45.0%
TTGCCTTTCTCAACTATCGC+AGG - chr1.3:69828770-69828789 MS.gene49054:CDS 45.0%
TTTCCTACGATATCCCTCTC+TGG + chr1.3:69826404-69826423 None:intergenic 45.0%
! AAACCCCCTTCCCAAACTTT+TGG - chr1.3:69828966-69828985 MS.gene49054:CDS 45.0%
! CGGACTCATGGTATGTGTTT+TGG + chr1.3:69828888-69828907 None:intergenic 45.0%
! CGTTCGGACTGCAACAATTT+TGG + chr1.3:69826162-69826181 None:intergenic 45.0%
! GCAGAACAGACAGAGCTTTT+CGG - chr1.3:69826953-69826972 MS.gene49054:CDS 45.0%
! GGCTTTGTCAACACAGTGTT+CGG - chr1.3:69826235-69826254 MS.gene49054:intron 45.0%
!! AGGGTTATCAGTTTGCTGTG+AGG - chr1.3:69828063-69828082 MS.gene49054:CDS 45.0%
!! CAGCTTGCTACAACCATCTT+AGG + chr1.3:69827984-69828003 None:intergenic 45.0%
AATTGACAGGACCGCTACAC+TGG - chr1.3:69828262-69828281 MS.gene49054:CDS 50.0%
ACTGGATAGGTGCCAACCAA+CGG - chr1.3:69828280-69828299 MS.gene49054:CDS 50.0%
AGAAGTTTGGCGGTTGGAAC+AGG + chr1.3:69828728-69828747 None:intergenic 50.0%
AGGAGAAGCTGGACGTTTGA+TGG - chr1.3:69826777-69826796 MS.gene49054:intron 50.0%
CACAGTGTTCGGAATCGGTA+CGG - chr1.3:69826246-69826265 MS.gene49054:intron 50.0%
CCACCAAAAGTTTGGGAAGG+GGG + chr1.3:69828972-69828991 None:intergenic 50.0%
CCCACCAAAAGTTTGGGAAG+GGG + chr1.3:69828973-69828992 None:intergenic 50.0%
CCGAACGTTCTTCACGCTAA+TGG - chr1.3:69826175-69826194 MS.gene49054:intron 50.0%
CTGGGCTGATGACAATATGG+TGG - chr1.3:69826885-69826904 MS.gene49054:CDS 50.0%
GGAGAAGCTGGACGTTTGAT+GGG - chr1.3:69826778-69826797 MS.gene49054:intron 50.0%
GGAGACGAGAACTGGATGTT+TGG - chr1.3:69826459-69826478 MS.gene49054:CDS 50.0%
GGCAAGAGCTTCTGAATCTG+TGG - chr1.3:69828040-69828059 MS.gene49054:CDS 50.0%
GGGTTATCGGGTGTTTATGG+TGG - chr1.3:69828990-69829009 MS.gene49054:CDS 50.0%
TATTGCTTGGCACGGACTCA+TGG + chr1.3:69828900-69828919 None:intergenic 50.0%
TCCTCAGTCGCATGTCCATT+CGG + chr1.3:69828212-69828231 None:intergenic 50.0%
TGCTTCAGAAAGTGAGCCTG+CGG - chr1.3:69828178-69828197 MS.gene49054:CDS 50.0%
TTGGCACCTATCCAGTGTAG+CGG + chr1.3:69828276-69828295 None:intergenic 50.0%
! CCCCTTCCCAAACTTTTGGT+GGG - chr1.3:69828970-69828989 MS.gene49054:CDS 50.0%
! TTGTGTTGCTCAAGCTGTGG+AGG - chr1.3:69826609-69826628 MS.gene49054:intron 50.0%
!! GAGAAATGGGCTGTCTGCTT+TGG - chr1.3:69827390-69827409 MS.gene49054:CDS 50.0%
!! GGTGGGTTATCGGGTGTTTA+TGG - chr1.3:69828987-69829006 MS.gene49054:CDS 50.0%
ACAGGACCGCTACACTGGAT+AGG - chr1.3:69828267-69828286 MS.gene49054:CDS 55.0%
CATCGGAAGGAGACGAGAAC+TGG - chr1.3:69826451-69826470 MS.gene49054:CDS 55.0%
CCATTCGGAAAGATCACCGC+AGG + chr1.3:69828197-69828216 None:intergenic 55.0%
CCTGCGGTGATCTTTCCGAA+TGG - chr1.3:69828194-69828213 MS.gene49054:CDS 55.0%
GCTCCAGAGAGGGATATCGT+AGG - chr1.3:69826398-69826417 MS.gene49054:intron 55.0%
TCATGCTTGGGGGGTTATGC+TGG - chr1.3:69827314-69827333 MS.gene49054:CDS 55.0%
TCCGAATGGACATGCGACTG+AGG - chr1.3:69828208-69828227 MS.gene49054:CDS 55.0%
TGGACATGCGACTGAGGATC+AGG - chr1.3:69828214-69828233 MS.gene49054:CDS 55.0%
! CCCCCTTCCCAAACTTTTGG+TGG - chr1.3:69828969-69828988 MS.gene49054:CDS 55.0%
!! ACGATATCCCTCTCTGGAGC+AGG + chr1.3:69826398-69826417 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 69826067 69829137 69826067 ID=MS.gene49054
chr1.3 mRNA 69826067 69829137 69826067 ID=MS.gene49054.t1;Parent=MS.gene49054
chr1.3 exon 69826780 69829137 69826780 ID=MS.gene49054.t1.exon1;Parent=MS.gene49054.t1
chr1.3 CDS 69826780 69829137 69826780 ID=cds.MS.gene49054.t1;Parent=MS.gene49054.t1
chr1.3 exon 69826413 69826565 69826413 ID=MS.gene49054.t1.exon2;Parent=MS.gene49054.t1
chr1.3 CDS 69826413 69826565 69826413 ID=cds.MS.gene49054.t1;Parent=MS.gene49054.t1
chr1.3 exon 69826271 69826330 69826271 ID=MS.gene49054.t1.exon3;Parent=MS.gene49054.t1
chr1.3 CDS 69826271 69826330 69826271 ID=cds.MS.gene49054.t1;Parent=MS.gene49054.t1
chr1.3 exon 69826067 69826153 69826067 ID=MS.gene49054.t1.exon4;Parent=MS.gene49054.t1
chr1.3 CDS 69826067 69826153 69826067 ID=cds.MS.gene49054.t1;Parent=MS.gene49054.t1
Gene Sequence

>MS.gene49054

ATGGATGGTGATCATAACAACTTAGGTAATGTGCATGTAGAAAATGTTGATGTTGGACTTGGAACATCCAATAGTTTGGACTTGAATGTGGACCAAAATTGTTGCAGTCCGAACGTTCTTCACGCTAATGGCTCTCAAACTCAATCTGGTAATGCATCAGCAAATGGAGGCTTTGTCAACACAGTGTTCGGAATCGGTACGGTATTTGAGTCTGATGAACATGCATATAGATTCTATAACAAGTATGCCAGATTGATGGGGTTCAATGTTAGAAAAGATTGGATTAATAGGAGTAAGGTACATGGTATGGTGGTTTCTAGGAAATTTACCTGCTCCAGAGAGGGATATCGTAGGAAAGACAAGAGAGATTTTACTGTGAAGAAACATCGGAAGGAGACGAGAACTGGATGTTTGGCACACATGATCATTACTCGTCAACAAGATGGAAAATACCAGGTTACCCATTTTGAAGCACAACATAATCATGATGATATAAACCCAATCAATTCTAACATGTTGTTACTAGAGTTGCAAAATGAATTTTGTGTTGCTCAAGCTGTGGAGGTTGACTCATATCATAATTTGGGACCCAAATCTAGTTCAGCTTTAAGTATGACGAATACAAAATTAGGTGCACGTGATTCTCTTGATCAACTTTCCATGAATTATGACAATTATCTTCATTCTGTGAGGGAAAGAGATATGGGTAAAGGAGAAGCTGGACGTTTGATGGGTTATTTTCAAAGGCAACACTTTGAAAATCCAGCATTTTTTTATGCCATACAGCTCGATGTTGATGACAAGATAACAAATCTTTTCTGGGCTGATGACAATATGGTGGTAGATTACGACCATTTTGGGGATGTTGTTTGTTTGGATACAACTTGCAGAACAGACAGAGCTTTTCGGCCATTTGTACAGTTTTTAGGTGTTAATCATCACAAACAAGTATTAATATTTGCTGCTGCTTTTTTGTATGATGAGACCATCGAATCATTTAATTGGCTGTTTCGAACTTTCATTGGCGCAATGTCTGGGAAAAAGCCTAAGGCCATTATCACAGAACAAGATGCTGCAATCATCGAAGCAATCAATGTGGTTTTACCTGAAACAGACCGGTATACATGTGTTTGGCAGATGTATGAGAACACTCTTAAACATCTCAGCCATTTGGTTAAAGATGACGAATCCTTTGCAAATGATCTAAGGAGTTGTATATATGACCCCAAGGACGAGGAAGAATTTACTCATGCTTGGGGGGTTATGCTGGAGAAATATAAACTCCAGCGAAATGAATGGTTGAGATGGATGTATAGAGAAAGGGAGAAATGGGCTGTCTGCTTTGGTCAGAATAGATTTTTTGTAGATGTCAAAGGCTTTCATTTAGGTGAGATGTTGTCTCACAAGCTTAGAAGCTATCTAAATCCTGACCTTGATGTAGTTCAATTTCTTAACCATTTTGAAAGGATAGTGGAGGAGCAACGATATAAAGAAATAGAAGCTAGTGATGAAATGAAGGGATGTCTTCCAAAGCTAATGGGTAATGTGGTTGTGTTGAAACATGCTAGTGTTGCTTATACTCCAAGAGCATTTGAAATATTTCAGCAACGATATGAGAACTCTTTGAATGTTATTGTCAACCAGCACAAAAGAGATGGATATTTGTTTGAGTATAAAGTGAATACATTTGGACATGCTAGACAGTACACTGTCACCTTCAATTCTTCAGATAATACAGTTGTCTGCAGTTGTATGAAGTTTGAGCATGTAGGTTTTCTTTGTAGCCATGCACTAAAAGTTCTTGATAATAGAAACATAAAAGTAGTTCCATCTCGATATATCTTGAAGAGATGGACAAAAGATGCAAGGTTGGGTAATGTCAGGGAGATTAAGCAATTTAAAATTCAAGATAACCCTAAGATGGTTGTAGCAAGCTGTTACAAAGATTTATGCCACAGACTTGTCAAGTTATCGGCAAGAGCTTCTGAATCTGTGGAGGGTTATCAGTTTGCTGTGAGGCAACTTGACGAAGTTATGGAAGGAGTGCAGAAAATCTTGATATTGAAAAGTGAGGAAGCTCAAGTTATCACCTCAAATAGTATTCATGTAGATGCTTCAGAAAGTGAGCCTGCGGTGATCTTTCCGAATGGACATGCGACTGAGGATCAGGATGAAAGTAACAGAGTAAGCGAAGAAATTGACAGGACCGCTACACTGGATAGGTGCCAACCAACGGTGAATTATAATCAAACTGATTCAGATAGGATTTTAAATGTAGAACCTTCTTCAAATACTGTTGTCTGCATTTCATGCCCCCCATCTCCATATGTTTCATCTCAACCAAATCTCCCTTTGCAGGTAAGCTTTTATCATTTATTTGATTGATATATGGTAGCTTGTATTCTTGTGATCATATAGAGCATATTTGATATAGAGATCAGGAAGTTTAATGCTCTTTCTTTTTTTGCAATGAAGTTTTATGCTCGTTAACTTTTGGAGAATATGCTCTGTATAATTTTGAAATAAAAAATAAAGAGAACGGCATTTGTTGATTTTCTCATTTGTTTTACAGGGCTTATTTGGTTTTGAAACCAATGAAGTGGTTCAATGCATGTATGAACAGCCTGATCTTGTGTTAGATCATCAATCTAATACTAACCTGTTCCAACCGCCAAACTTCTATTCTAACCAACAGAGTTCTCCTTGCCTTTCTCAACTATCGCAGGTGTTAATTTTTCCAGTCATTAGGTTTTTTTTATATGCAGAATATGTTACTTTGTATAACATATCATGAATATTTTACTCAGGAGCCTATAATCCAAAACACATACCATGAGTCCGTGCCAAGCAATAATCAGATGCAACAGGTAAACATTTACTTAATGGTCATAATTTGAACAAACCCCCTTCCCAAACTTTTGGTGGGTTATCGGGTGTTTATGGTGGAAAACTAATTGCATCTGCTTTGTGTATATTTGCTGTAGGGTATGGATCTTGATATCCAAAATCCTCATTCAGAATCCTGTATTTTGCTGTGTGACCATAGATACCGAAGTTCTGACTCTGCATAA
Protein sequence

>MS.gene49054.t1

MDGDHNNLGNVHVENVDVGLGTSNSLDLNVDQNCCSPNVLHANGSQTQSGNASANGGFVNTVFGIGTVFESDEHAYRFYNKYARLMGFNVRKDWINRSKVHGMVVSRKFTCSREGYRRKDKRDFTVKKHRKETRTGCLAHMIITRQQDGKYQVTHFEAQHNHDDINPINSNMLLLELQNEFCVAQAVEVDSYHNLGPKSSSALSMTNTKLGARDSLDQLSMNYDNYLHSVRERDMGKGEAGRLMGYFQRQHFENPAFFYAIQLDVDDKITNLFWADDNMVVDYDHFGDVVCLDTTCRTDRAFRPFVQFLGVNHHKQVLIFAAAFLYDETIESFNWLFRTFIGAMSGKKPKAIITEQDAAIIEAINVVLPETDRYTCVWQMYENTLKHLSHLVKDDESFANDLRSCIYDPKDEEEFTHAWGVMLEKYKLQRNEWLRWMYREREKWAVCFGQNRFFVDVKGFHLGEMLSHKLRSYLNPDLDVVQFLNHFERIVEEQRYKEIEASDEMKGCLPKLMGNVVVLKHASVAYTPRAFEIFQQRYENSLNVIVNQHKRDGYLFEYKVNTFGHARQYTVTFNSSDNTVVCSCMKFEHVGFLCSHALKVLDNRNIKVVPSRYILKRWTKDARLGNVREIKQFKIQDNPKMVVASCYKDLCHRLVKLSARASESVEGYQFAVRQLDEVMEGVQKILILKSEEAQVITSNSIHVDASESEPAVIFPNGHATEDQDESNRVSEEIDRTATLDRCQPTVNYNQTDSDRILNVEPSSNTVVCISCPPSPYVSSQPNLPLQGLFGFETNEVVQCMYEQPDLVLDHQSNTNLFQPPNFYSNQQSSPCLSQLSQEPIIQNTYHESVPSNNQMQQGMDLDIQNPHSESCILLCDHRYRSSDSA