AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49174

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49174.t1 MTR_6g053640 55.645 124 53 2 364 485 2 125 1.87e-34 126
MS.gene49174.t1 MTR_3g005220 59.406 101 37 2 8 107 28 125 8.33e-32 120
MS.gene49174.t1 MTR_6g024175 65.854 82 27 1 385 465 103 184 2.11e-26 106
MS.gene49174.t1 MTR_2g098880 44.954 109 59 1 52 159 59 167 7.37e-25 103
MS.gene49174.t1 MTR_2g098880 44.954 109 59 1 52 159 71 179 1.05e-24 103
MS.gene49174.t1 MTR_2g098920 38.596 114 58 3 53 161 79 185 6.92e-19 86.7
MS.gene49174.t1 MTR_4g067290 33.582 134 77 3 28 156 30 156 2.83e-18 84.3
MS.gene49174.t1 MTR_4g067290 33.582 134 77 3 28 156 30 156 2.83e-18 84.3
MS.gene49174.t1 MTR_4g067290 33.582 134 77 3 28 156 50 176 4.05e-18 84.3
MS.gene49174.t1 MTR_4g067290 33.582 134 77 3 28 156 50 176 4.19e-18 84.3
MS.gene49174.t1 MTR_4g067290 33.582 134 77 3 28 156 48 174 4.32e-18 84.3
MS.gene49174.t1 MTR_4g067290 33.582 134 77 3 28 156 90 216 5.57e-18 84.7
MS.gene49174.t1 MTR_3g006370 32.738 168 107 5 18 181 12 177 5.86e-16 77.4
MS.gene49174.t1 MTR_0853s0010 29.801 151 89 5 52 195 23 163 9.55e-13 66.6
MS.gene49174.t1 MTR_0402s0040 27.354 223 133 8 50 251 26 240 9.64e-13 70.5
MS.gene49174.t1 MTR_1g070355 29.054 148 100 3 59 202 79 225 1.40e-12 70.5
MS.gene49174.t1 MTR_1g019850 32.903 155 88 7 38 187 53 196 7.54e-12 65.5
MS.gene49174.t1 MTR_4g035820 32.143 140 86 4 54 187 75 211 9.68e-12 67.8
MS.gene49174.t1 MTR_7g074790 34.815 135 68 4 53 187 9 123 4.24e-11 63.2
MS.gene49174.t1 MTR_6g022070 31.667 120 81 1 200 318 50 169 5.72e-11 64.3
MS.gene49174.t1 MTR_6g060130 49.206 63 31 1 15 76 60 122 9.96e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49174.t1 AT4G38180 42.294 279 156 5 196 471 419 695 1.73e-60 212
MS.gene49174.t1 AT4G38180 41.447 152 85 3 52 199 71 222 6.83e-24 105
MS.gene49174.t1 AT4G38170 33.824 272 178 1 198 469 187 456 1.71e-48 175
MS.gene49174.t1 AT2G27110 33.455 275 174 4 200 472 226 493 1.80e-47 175
MS.gene49174.t1 AT2G27110 33.455 275 174 4 200 472 371 638 3.90e-47 175
MS.gene49174.t1 AT2G27110 33.455 275 174 4 200 472 371 638 3.90e-47 175
MS.gene49174.t1 AT5G18960 41.232 211 121 1 200 410 521 728 3.30e-45 169
MS.gene49174.t1 AT5G18960 35.238 105 52 2 53 156 210 299 5.45e-13 72.0
MS.gene49174.t1 AT5G18960 32.075 106 56 2 52 156 41 131 1.27e-12 70.9
MS.gene49174.t1 AT5G18960 41.232 211 121 1 200 410 521 728 3.30e-45 169
MS.gene49174.t1 AT5G18960 35.238 105 52 2 53 156 210 299 5.45e-13 72.0
MS.gene49174.t1 AT5G18960 32.075 106 56 2 52 156 41 131 1.27e-12 70.9
MS.gene49174.t1 AT3G06250 40.284 211 123 1 200 410 497 704 1.96e-44 167
MS.gene49174.t1 AT3G06250 32.456 114 61 2 44 156 18 116 1.88e-13 73.6
MS.gene49174.t1 AT3G06250 34.483 116 59 3 43 156 178 278 2.08e-13 73.2
MS.gene49174.t1 AT3G06250 40.284 211 123 1 200 410 497 704 1.96e-44 167
MS.gene49174.t1 AT3G06250 32.456 114 61 2 44 156 18 116 1.88e-13 73.6
MS.gene49174.t1 AT3G06250 34.483 116 59 3 43 156 178 278 2.08e-13 73.2
MS.gene49174.t1 AT3G06250 40.284 211 123 1 200 410 497 704 1.96e-44 167
MS.gene49174.t1 AT3G06250 32.456 114 61 2 44 156 18 116 1.88e-13 73.6
MS.gene49174.t1 AT3G06250 34.483 116 59 3 43 156 178 278 2.08e-13 73.2
MS.gene49174.t1 AT3G22170 30.970 268 178 2 200 467 409 669 5.11e-44 166
MS.gene49174.t1 AT3G22170 30.970 268 178 2 200 467 409 669 5.11e-44 166
MS.gene49174.t1 AT4G15090 32.955 264 169 3 200 462 400 656 3.65e-43 163
MS.gene49174.t1 AT4G15090 31.061 132 87 3 52 182 49 177 8.92e-11 65.1
MS.gene49174.t1 AT4G15090 32.955 264 169 3 200 462 400 656 3.65e-43 163
MS.gene49174.t1 AT4G15090 31.061 132 87 3 52 182 49 177 8.92e-11 65.1
MS.gene49174.t1 AT4G15090 32.955 264 169 3 200 462 400 656 3.65e-43 163
MS.gene49174.t1 AT4G15090 31.061 132 87 3 52 182 49 177 8.92e-11 65.1
MS.gene49174.t1 AT4G15090 32.955 264 169 3 200 462 400 656 3.65e-43 163
MS.gene49174.t1 AT4G15090 31.061 132 87 3 52 182 49 177 8.92e-11 65.1
MS.gene49174.t1 AT4G15090 32.955 264 169 3 200 462 400 656 3.65e-43 163
MS.gene49174.t1 AT4G15090 31.061 132 87 3 52 182 49 177 8.92e-11 65.1
MS.gene49174.t1 AT1G52520 29.254 335 213 8 155 473 373 699 1.63e-37 146
MS.gene49174.t1 AT4G19990 29.808 312 201 5 173 477 270 570 1.12e-34 138
MS.gene49174.t1 AT4G19990 29.808 312 201 5 173 477 327 627 1.23e-34 138
MS.gene49174.t1 AT4G19990 29.808 312 201 5 173 477 327 627 1.51e-34 138
MS.gene49174.t1 AT4G19990 29.808 312 201 5 173 477 327 627 1.51e-34 138
MS.gene49174.t1 AT4G19990 29.808 312 201 5 173 477 341 641 1.51e-34 138
MS.gene49174.t1 AT4G19990 29.808 312 201 5 173 477 327 627 1.53e-34 138
MS.gene49174.t1 AT4G19990 29.808 312 201 5 173 477 327 627 1.53e-34 138
MS.gene49174.t1 AT1G76320 30.855 269 178 3 200 468 337 597 5.61e-34 136
MS.gene49174.t1 AT1G76320 30.855 269 178 3 200 468 337 597 5.64e-34 136
MS.gene49174.t1 AT1G76320 30.855 269 178 3 200 468 337 597 5.64e-34 136
MS.gene49174.t1 AT1G76320 30.855 269 178 3 200 468 337 597 6.06e-34 135
MS.gene49174.t1 AT2G32250 32.093 215 143 2 201 413 345 558 1.14e-30 126
MS.gene49174.t1 AT2G32250 32.093 215 143 2 201 413 345 558 1.14e-30 126
MS.gene49174.t1 AT2G32250 32.093 215 143 2 201 413 345 558 1.16e-30 126
MS.gene49174.t1 AT2G32250 32.093 215 143 2 201 413 345 558 1.16e-30 126
MS.gene49174.t1 AT2G32250 32.093 215 143 2 201 413 345 558 1.16e-30 126
MS.gene49174.t1 AT2G32250 32.093 215 143 2 201 413 304 517 1.57e-30 126
MS.gene49174.t1 AT2G32250 32.093 215 143 2 201 413 304 517 1.71e-30 125
MS.gene49174.t1 AT2G32250 32.093 215 143 2 201 413 345 558 1.72e-30 125
MS.gene49174.t1 AT1G80010 27.622 286 194 5 195 472 440 720 3.74e-30 124
MS.gene49174.t1 AT5G28530 26.038 265 190 2 200 464 417 675 1.08e-26 114
MS.gene49174.t1 AT5G28530 26.415 265 189 3 200 464 354 612 1.10e-26 114
MS.gene49174.t1 AT5G28530 25.283 265 188 2 200 464 417 671 1.10e-25 111
MS.gene49174.t1 AT5G28530 25.283 265 188 2 200 464 417 671 1.10e-25 111
MS.gene49174.t1 AT5G28530 25.660 265 187 3 200 464 354 608 1.11e-25 110
MS.gene49174.t1 AT1G10240 29.612 206 140 1 200 405 402 602 3.48e-23 103
MS.gene49174.t1 AT1G10240 29.612 206 140 1 200 405 402 602 3.48e-23 103
MS.gene49174.t1 AT3G59470 38.053 113 58 3 53 160 69 174 1.12e-18 85.9
MS.gene49174.t1 AT3G59470 38.053 113 58 3 53 160 69 174 1.12e-18 85.9
MS.gene49174.t1 AT3G59470 38.053 113 58 3 53 160 81 186 1.39e-18 85.9
MS.gene49174.t1 AT3G07500 36.036 111 68 2 48 157 27 135 4.17e-15 74.7
MS.gene49174.t1 AT2G43280 34.959 123 77 3 43 163 11 132 1.17e-13 70.5
MS.gene49174.t1 AT4G12850 34.286 105 63 2 53 156 10 109 8.66e-13 66.2
MS.gene49174.t1 AT4G12850 34.286 105 63 2 53 156 10 109 9.55e-13 67.0
MS.gene49174.t1 AT4G12850 34.286 105 63 2 53 156 10 109 1.23e-12 65.9

Find 70 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCTTCAATGGGCTCTA+AGG 0.236305 1.3:+68490005 None:intergenic
CAATACTGTTTGCGTTATTA+TGG 0.247980 1.3:-68490142 MS.gene49174:CDS
TGAAAAGTCACATGATATTT+AGG 0.266210 1.3:+68488491 None:intergenic
CGAAGAGACTGTGCACTTCT+AGG 0.276999 1.3:+68489682 None:intergenic
AACTGTGTTCCTAAAGAAAA+AGG 0.312218 1.3:-68488396 MS.gene49174:CDS
AATCGTTTGGTATTCTTTGT+AGG 0.315386 1.3:-68488427 MS.gene49174:CDS
TATACTCAATTCGATCAAAA+TGG 0.317025 1.3:-68488883 MS.gene49174:CDS
TTCATTGTTTAATAGTGTTA+TGG 0.327096 1.3:-68488258 MS.gene49174:CDS
ACTGATGTGCTTGATGAATC+TGG 0.354525 1.3:-68489622 MS.gene49174:CDS
GAGAAAACAGCTGGTGATTA+TGG 0.357013 1.3:+68489704 None:intergenic
TGTCAAAAGTTTGAATCGTT+TGG 0.358658 1.3:-68488440 MS.gene49174:CDS
TTTAAACTCCATTCCTTCAA+TGG 0.364249 1.3:+68489996 None:intergenic
AGAACAAACGGTATGATAAT+TGG 0.370543 1.3:-68489889 MS.gene49174:CDS
TAGTGTTATGGTTCGTTCAC+TGG 0.382958 1.3:-68488246 MS.gene49174:CDS
ATTCCTTCAATGGGCTCTAA+GGG 0.384475 1.3:+68490006 None:intergenic
TTGAAGAATTTGAGTCTGAA+TGG 0.400537 1.3:-68488958 MS.gene49174:intron
TTAAACTCCATTCCTTCAAT+GGG 0.435121 1.3:+68489997 None:intergenic
GTGCTTGATGAATCTGGTTT+AGG 0.447135 1.3:-68489616 MS.gene49174:CDS
TTGACAATTGAAGAATTTGA+AGG 0.456352 1.3:-68488302 MS.gene49174:CDS
TGAAGAATTTGAGTCTGAAT+GGG 0.463681 1.3:-68488957 MS.gene49174:intron
TCCTTCAATGGGCTCTAAGG+GGG 0.466966 1.3:+68490008 None:intergenic
CTGGAATGTCTACCACTCAT+AGG 0.472850 1.3:-68488829 MS.gene49174:CDS
TTTGGCTCGAAACCCCTCCT+TGG 0.475441 1.3:+68489849 None:intergenic
TTTGAAGCTTCTTCTTCCAT+GGG 0.477189 1.3:+68488644 None:intergenic
ACTGTTTGCGTTATTATGGC+AGG 0.480651 1.3:-68490138 MS.gene49174:CDS
GAAGCAATGATTGATAAATA+CGG 0.502447 1.3:-68488935 MS.gene49174:CDS
AAATTATGATGTACATATGC+AGG 0.504291 1.3:+68488857 None:intergenic
GGGTATAAAGTTCGCAAAAG+TGG 0.509142 1.3:+68488665 None:intergenic
TCAAGAAATTCTTTGTTCCA+AGG 0.511520 1.3:-68489866 MS.gene49174:CDS
CCTTCAATGGGCTCTAAGGG+GGG 0.511902 1.3:+68490009 None:intergenic
AAGCAATGATTGATAAATAC+GGG 0.513517 1.3:-68488934 MS.gene49174:CDS
CACAGTATGTTCTACAACGA+TGG 0.518628 1.3:-68488358 MS.gene49174:CDS
TGTGTCAGAAACTTGCTCAA+TGG 0.523310 1.3:+68488758 None:intergenic
CTTAGAGCCCATTGAAGGAA+TGG 0.526994 1.3:-68490004 MS.gene49174:CDS
ACAATTGAAGAATTTGAAGG+AGG 0.530417 1.3:-68488299 MS.gene49174:CDS
CACTTCTAGGAGAAAACAGC+TGG 0.530595 1.3:+68489695 None:intergenic
TGAGATATTTCAAGATGAGT+TGG 0.531340 1.3:-68488600 MS.gene49174:CDS
GTTACTAAGTCAAGAACAAA+CGG 0.531428 1.3:-68489901 MS.gene49174:CDS
AAATTCTTTGTTCCAAGGAG+GGG 0.533751 1.3:-68489861 MS.gene49174:CDS
AGGTCATGAGATGACAGATA+TGG 0.541561 1.3:-68490118 MS.gene49174:CDS
GATGGTGGTTCACTATTGAG+AGG 0.542446 1.3:+68490036 None:intergenic
ATATTTCAAGATGAGTTGGT+TGG 0.542621 1.3:-68488596 MS.gene49174:CDS
GAAATTCTTTGTTCCAAGGA+GGG 0.547884 1.3:-68489862 MS.gene49174:CDS
TGCACGTTACAACGAGGAAA+GGG 0.548977 1.3:-68488717 MS.gene49174:CDS
TGAAACCAGGGTGGGATGCA+AGG 0.555494 1.3:-68489785 MS.gene49174:CDS
TCTGTCTTATGCACTGAAAG+TGG 0.562246 1.3:-68489583 MS.gene49174:CDS
TTCCTTCAATGGGCTCTAAG+GGG 0.577393 1.3:+68490007 None:intergenic
CCGTGCCTTGCATCCCACCC+TGG 0.580963 1.3:+68489780 None:intergenic
TTTGCGAACTTTATACCCCA+TGG 0.583317 1.3:-68488660 MS.gene49174:CDS
ATGCACGTTACAACGAGGAA+AGG 0.585794 1.3:-68488718 MS.gene49174:CDS
CGTTGTAGAACATACTGTGA+AGG 0.588365 1.3:+68488362 None:intergenic
CATGAGATGACAGATATGGA+TGG 0.601625 1.3:-68490114 MS.gene49174:CDS
AGAAATTCTTTGTTCCAAGG+AGG 0.606491 1.3:-68489863 MS.gene49174:CDS
TTGAAGCTTCTTCTTCCATG+GGG 0.606500 1.3:+68488645 None:intergenic
GTTGTAGAACATACTGTGAA+GGG 0.621697 1.3:+68488363 None:intergenic
AGAGCTTGAAGAATCAAATG+AGG 0.626048 1.3:-68488135 MS.gene49174:CDS
TTATTACACATGATGAAACC+AGG 0.637175 1.3:-68489798 MS.gene49174:CDS
CATACTTTCACTCCTATGAG+TGG 0.639566 1.3:+68488817 None:intergenic
ACAAGGTACGCTCAGAACAA+AGG 0.639870 1.3:-68489946 MS.gene49174:CDS
CCAGGGTGGGATGCAAGGCA+CGG 0.641442 1.3:-68489780 MS.gene49174:CDS
ACGGGTTGCAAGATAATCAG+TGG 0.648204 1.3:-68488916 MS.gene49174:CDS
AAGCAAAGAGTTACTACACA+AGG 0.651539 1.3:-68489963 MS.gene49174:CDS
ACACATGATGAAACCAGGGT+GGG 0.653979 1.3:-68489793 MS.gene49174:CDS
ACTTAGTAACACGGCCCATG+CGG 0.656322 1.3:+68489914 None:intergenic
GTCTTATGCACTGAAAGTGG+CGG 0.656902 1.3:-68489580 MS.gene49174:intron
TTGTTCTTGACTTAGTAACA+CGG 0.665948 1.3:+68489905 None:intergenic
TACACATGATGAAACCAGGG+TGG 0.688016 1.3:-68489794 MS.gene49174:CDS
TATTACACATGATGAAACCA+GGG 0.694710 1.3:-68489797 MS.gene49174:CDS
TGTATCATTTGAGACAACCA+TGG 0.701765 1.3:+68490083 None:intergenic
TCTTGATGCACGTTACAACG+AGG 0.755842 1.3:-68488723 MS.gene49174:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAGAAAAATAATGATATA+TGG - chr1.3:68488518-68488537 MS.gene49174:CDS 10.0%
!!! AAAATTAACTAATTGCTTTT+GGG - chr1.3:68488876-68488895 MS.gene49174:CDS 15.0%
!!! AAGTATGAATAAGTTTTTTA+AGG - chr1.3:68489467-68489486 MS.gene49174:intron 15.0%
!!! TATTTATTTTTCAAGTATGT+TGG + chr1.3:68488958-68488977 None:intergenic 15.0%
!! TTCATTGTTTAATAGTGTTA+TGG - chr1.3:68490010-68490029 MS.gene49174:CDS 20.0%
!!! AAATATCTCAAACAATTTTC+CGG + chr1.3:68489658-68489677 None:intergenic 20.0%
!!! AATAATTGATAACATCTTGT+TGG + chr1.3:68488735-68488754 None:intergenic 20.0%
!!! ATTTTTCTTTCTTAGCATTT+TGG + chr1.3:68489938-68489957 None:intergenic 20.0%
!!! GAAAATTAACTAATTGCTTT+TGG - chr1.3:68488875-68488894 MS.gene49174:CDS 20.0%
!!! TTTTTCTTTCTTAGCATTTT+GGG + chr1.3:68489937-68489956 None:intergenic 20.0%
! AAATTATGATGTACATATGC+AGG + chr1.3:68489414-68489433 None:intergenic 25.0%
! AAGCAATGATTGATAAATAC+GGG - chr1.3:68489334-68489353 MS.gene49174:intron 25.0%
! ATTCTTCAATTGTGATAGAT+TGG + chr1.3:68489299-68489318 None:intergenic 25.0%
! GAAGCAATGATTGATAAATA+CGG - chr1.3:68489333-68489352 MS.gene49174:intron 25.0%
! GAAGCTTCAAAAATTTATAC+CGG - chr1.3:68489636-68489655 MS.gene49174:CDS 25.0%
! GCTAAGAAAGAAAAATTTGA+AGG - chr1.3:68489942-68489961 MS.gene49174:CDS 25.0%
! TATACTCAATTCGATCAAAA+TGG - chr1.3:68489385-68489404 MS.gene49174:intron 25.0%
! TGAAAAGTCACATGATATTT+AGG + chr1.3:68489780-68489799 None:intergenic 25.0%
! TTGACAATTGAAGAATTTGA+AGG - chr1.3:68489966-68489985 MS.gene49174:CDS 25.0%
!! AGAGTTGCTTACAAATATTT+TGG - chr1.3:68488913-68488932 MS.gene49174:CDS 25.0%
!! CATCAACAATCAATTCTTTT+TGG - chr1.3:68489015-68489034 MS.gene49174:intron 25.0%
!! GCAATTAGTTAATTTTCCTT+CGG + chr1.3:68488872-68488891 None:intergenic 25.0%
!!! AGAAAATTCAACTTTTTCTG+TGG + chr1.3:68489712-68489731 None:intergenic 25.0%
!!! TTACAAATATTTTGGTGATG+TGG - chr1.3:68488921-68488940 MS.gene49174:CDS 25.0%
AACTGTGTTCCTAAAGAAAA+AGG - chr1.3:68489872-68489891 MS.gene49174:CDS 30.0%
AATCGTTTGGTATTCTTTGT+AGG - chr1.3:68489841-68489860 MS.gene49174:CDS 30.0%
AATTGTCAGTTGAAGAATAC+TGG - chr1.3:68488820-68488839 MS.gene49174:CDS 30.0%
ACAATTGAAGAATTTGAAGG+AGG - chr1.3:68489969-68489988 MS.gene49174:CDS 30.0%
AGAACAAACGGTATGATAAT+TGG - chr1.3:68488379-68488398 MS.gene49174:CDS 30.0%
ATTGTCAGTTGAAGAATACT+GGG - chr1.3:68488821-68488840 MS.gene49174:CDS 30.0%
CAATAATGGTGATTAACCTT+TGG + chr1.3:68489183-68489202 None:intergenic 30.0%
CAATACTGTTTGCGTTATTA+TGG - chr1.3:68488126-68488145 MS.gene49174:CDS 30.0%
GTTACTAAGTCAAGAACAAA+CGG - chr1.3:68488367-68488386 MS.gene49174:CDS 30.0%
TATTACACATGATGAAACCA+GGG - chr1.3:68488471-68488490 MS.gene49174:CDS 30.0%
TCAAGAAATTCTTTGTTCCA+AGG - chr1.3:68488402-68488421 MS.gene49174:CDS 30.0%
TGAAGAATTTGAGTCTGAAT+GGG - chr1.3:68489311-68489330 MS.gene49174:intron 30.0%
TGAGATATTTCAAGATGAGT+TGG - chr1.3:68489668-68489687 MS.gene49174:CDS 30.0%
TGATGATTAACTCCAGTAAT+TGG + chr1.3:68488997-68489016 None:intergenic 30.0%
TGTCAAAAGTTTGAATCGTT+TGG - chr1.3:68489828-68489847 MS.gene49174:CDS 30.0%
TTAAACTCCATTCCTTCAAT+GGG + chr1.3:68488274-68488293 None:intergenic 30.0%
TTAATCACCATTATTGCATG+TGG - chr1.3:68489187-68489206 MS.gene49174:intron 30.0%
TTATTACACATGATGAAACC+AGG - chr1.3:68488470-68488489 MS.gene49174:CDS 30.0%
TTGAAGAATTTGAGTCTGAA+TGG - chr1.3:68489310-68489329 MS.gene49174:intron 30.0%
TTGTTCTTGACTTAGTAACA+CGG + chr1.3:68488366-68488385 None:intergenic 30.0%
TTTAAACTCCATTCCTTCAA+TGG + chr1.3:68488275-68488294 None:intergenic 30.0%
! AGTTGAATTTTCTGATGAAG+TGG - chr1.3:68489719-68489738 MS.gene49174:CDS 30.0%
! GATAACGTACCTTTTTCTTT+AGG + chr1.3:68489884-68489903 None:intergenic 30.0%
! GTACATCATAATTTTTGTGC+TGG - chr1.3:68489420-68489439 MS.gene49174:intron 30.0%
! GTTTTTCTATGCGTTTCAAA+TGG - chr1.3:68488843-68488862 MS.gene49174:CDS 30.0%
! TGGTCTGTGATAATAGTTTT+AGG + chr1.3:68489123-68489142 None:intergenic 30.0%
!! AATTTTCTGAAAGAGCTTCA+TGG - chr1.3:68490045-68490064 MS.gene49174:CDS 30.0%
!! ATATTTCAAGATGAGTTGGT+TGG - chr1.3:68489672-68489691 MS.gene49174:CDS 30.0%
!! GGTCTGTGATAATAGTTTTA+GGG + chr1.3:68489122-68489141 None:intergenic 30.0%
!! TCCTTGTTTCACATACTTTT+TGG + chr1.3:68488440-68488459 None:intergenic 30.0%
!!! AAACTGTAGAGAGTTTTGTT+TGG - chr1.3:68489058-68489077 MS.gene49174:intron 30.0%
AAATTCTTTGTTCCAAGGAG+GGG - chr1.3:68488407-68488426 MS.gene49174:CDS 35.0%
AAGCAAAGAGTTACTACACA+AGG - chr1.3:68488305-68488324 MS.gene49174:CDS 35.0%
ACTCCAGTAATTGGAACAAA+AGG + chr1.3:68488988-68489007 None:intergenic 35.0%
ACTTGGTTAGAAGCAATGAA+TGG - chr1.3:68489090-68489109 MS.gene49174:intron 35.0%
AGAAATTCTTTGTTCCAAGG+AGG - chr1.3:68488405-68488424 MS.gene49174:CDS 35.0%
AGAGCTTGAAGAATCAAATG+AGG - chr1.3:68490133-68490152 MS.gene49174:CDS 35.0%
CAATATGCCACATGCAATAA+TGG + chr1.3:68489197-68489216 None:intergenic 35.0%
CTTGGTTAGAAGCAATGAAT+GGG - chr1.3:68489091-68489110 MS.gene49174:intron 35.0%
GAAATTCTTTGTTCCAAGGA+GGG - chr1.3:68488406-68488425 MS.gene49174:CDS 35.0%
GTTGTAGAACATACTGTGAA+GGG + chr1.3:68489908-68489927 None:intergenic 35.0%
TTATTGCATGTGGCATATTG+AGG - chr1.3:68489197-68489216 MS.gene49174:intron 35.0%
TTGGTTAGAAGCAATGAATG+GGG - chr1.3:68489092-68489111 MS.gene49174:intron 35.0%
TTGTTTGGTTGCTAAGTACT+TGG - chr1.3:68489073-68489092 MS.gene49174:intron 35.0%
! ACTCCTTTTGTTCCAATTAC+TGG - chr1.3:68488982-68489001 MS.gene49174:intron 35.0%
! GCCAAAAAGTATGTGAAACA+AGG - chr1.3:68488436-68488455 MS.gene49174:CDS 35.0%
! TATGGATGGAGATTTTACCA+TGG - chr1.3:68488168-68488187 MS.gene49174:CDS 35.0%
! TGTATCATTTGAGACAACCA+TGG + chr1.3:68488188-68488207 None:intergenic 35.0%
! TTTGAAGCTTCTTCTTCCAT+GGG + chr1.3:68489627-68489646 None:intergenic 35.0%
!!! AAAGGTTTTAGTTTCCGCAT+GGG - chr1.3:68488340-68488359 MS.gene49174:CDS 35.0%
!!! AGTGGCGGTATTGATAATTT+TGG - chr1.3:68488703-68488722 MS.gene49174:CDS 35.0%
!!! ATTGCTTTTGGGTTGATTCA+AGG - chr1.3:68488887-68488906 MS.gene49174:CDS 35.0%
!!! TTTTGAAGCTTCTTCTTCCA+TGG + chr1.3:68489628-68489647 None:intergenic 35.0%
ACTGATGTGCTTGATGAATC+TGG - chr1.3:68488646-68488665 MS.gene49174:CDS 40.0%
ACTGTTTGCGTTATTATGGC+AGG - chr1.3:68488130-68488149 MS.gene49174:CDS 40.0%
AGGTCATGAGATGACAGATA+TGG - chr1.3:68488150-68488169 MS.gene49174:CDS 40.0%
CACAGTATGTTCTACAACGA+TGG - chr1.3:68489910-68489929 MS.gene49174:CDS 40.0%
CACATGAATCAGATCTATCG+TGG - chr1.3:68489234-68489253 MS.gene49174:intron 40.0%
CATACTTTCACTCCTATGAG+TGG + chr1.3:68489454-68489473 None:intergenic 40.0%
CATGAGATGACAGATATGGA+TGG - chr1.3:68488154-68488173 MS.gene49174:CDS 40.0%
CGTTGTAGAACATACTGTGA+AGG + chr1.3:68489909-68489928 None:intergenic 40.0%
GAGAAAACAGCTGGTGATTA+TGG + chr1.3:68488567-68488586 None:intergenic 40.0%
GGGTATAAAGTTCGCAAAAG+TGG + chr1.3:68489606-68489625 None:intergenic 40.0%
TAGATCTGATTCATGTGCTC+TGG + chr1.3:68489231-68489250 None:intergenic 40.0%
TAGTGTTATGGTTCGTTCAC+TGG - chr1.3:68490022-68490041 MS.gene49174:CDS 40.0%
TATCACCAATGCAGTTGCAA+GGG - chr1.3:68489152-68489171 MS.gene49174:intron 40.0%
TCTGTCTTATGCACTGAAAG+TGG - chr1.3:68488685-68488704 MS.gene49174:CDS 40.0%
TTGAAGCTTCTTCTTCCATG+GGG + chr1.3:68489626-68489645 None:intergenic 40.0%
TTTGCGAACTTTATACCCCA+TGG - chr1.3:68489608-68489627 MS.gene49174:CDS 40.0%
! ATTCCTTCAATGGGCTCTAA+GGG + chr1.3:68488265-68488284 None:intergenic 40.0%
! GTGCTTGATGAATCTGGTTT+AGG - chr1.3:68488652-68488671 MS.gene49174:CDS 40.0%
! TGTGTCAGAAACTTGCTCAA+TGG + chr1.3:68489513-68489532 None:intergenic 40.0%
!! TTTTGGTTGTGCTCTCTTGT+GGG - chr1.3:68489032-68489051 MS.gene49174:intron 40.0%
!!! CAAAGGTTTTAGTTTCCGCA+TGG - chr1.3:68488339-68488358 MS.gene49174:CDS 40.0%
!!! TTTTTGGTTGTGCTCTCTTG+TGG - chr1.3:68489031-68489050 MS.gene49174:intron 40.0%
AAACACCCTTGCAACTGCAT+TGG + chr1.3:68489160-68489179 None:intergenic 45.0%
ACAAGGTACGCTCAGAACAA+AGG - chr1.3:68488322-68488341 MS.gene49174:CDS 45.0%
ACACATGATGAAACCAGGGT+GGG - chr1.3:68488475-68488494 MS.gene49174:CDS 45.0%
ATGCACGTTACAACGAGGAA+AGG - chr1.3:68489550-68489569 MS.gene49174:intron 45.0%
CTATCACCAATGCAGTTGCA+AGG - chr1.3:68489151-68489170 MS.gene49174:intron 45.0%
CTGGAATGTCTACCACTCAT+AGG - chr1.3:68489439-68489458 MS.gene49174:intron 45.0%
CTTAGAGCCCATTGAAGGAA+TGG - chr1.3:68488264-68488283 MS.gene49174:CDS 45.0%
GTCTTATGCACTGAAAGTGG+CGG - chr1.3:68488688-68488707 MS.gene49174:CDS 45.0%
TACACATGATGAAACCAGGG+TGG - chr1.3:68488474-68488493 MS.gene49174:CDS 45.0%
TCTTGATGCACGTTACAACG+AGG - chr1.3:68489545-68489564 MS.gene49174:intron 45.0%
TGCACGTTACAACGAGGAAA+GGG - chr1.3:68489551-68489570 MS.gene49174:intron 45.0%
! ACGGGTTGCAAGATAATCAG+TGG - chr1.3:68489352-68489371 MS.gene49174:intron 45.0%
! CACTTCTAGGAGAAAACAGC+TGG + chr1.3:68488576-68488595 None:intergenic 45.0%
! CATTCCTTCAATGGGCTCTA+AGG + chr1.3:68488266-68488285 None:intergenic 45.0%
! CATTGGTGATAGCAGTGTGT+TGG + chr1.3:68489143-68489162 None:intergenic 45.0%
! GATGGTGGTTCACTATTGAG+AGG + chr1.3:68488235-68488254 None:intergenic 45.0%
!! AGTTGCAAGGGTGTTTCCAA+AGG - chr1.3:68489164-68489183 MS.gene49174:intron 45.0%
!! TTCCTTCAATGGGCTCTAAG+GGG + chr1.3:68488264-68488283 None:intergenic 45.0%
ACTTAGTAACACGGCCCATG+CGG + chr1.3:68488357-68488376 None:intergenic 50.0%
CGAAGAGACTGTGCACTTCT+AGG + chr1.3:68488589-68488608 None:intergenic 50.0%
GCGTTTCAAATGGATGCCGA+AGG - chr1.3:68488853-68488872 MS.gene49174:CDS 50.0%
!! TCCTTCAATGGGCTCTAAGG+GGG + chr1.3:68488263-68488282 None:intergenic 50.0%
TGAAACCAGGGTGGGATGCA+AGG - chr1.3:68488483-68488502 MS.gene49174:CDS 55.0%
! TTTGGCTCGAAACCCCTCCT+TGG + chr1.3:68488422-68488441 None:intergenic 55.0%
!! CCTTCAATGGGCTCTAAGGG+GGG + chr1.3:68488262-68488281 None:intergenic 55.0%
CCCCCCTTAGAGCCCATTGA+AGG - chr1.3:68488259-68488278 MS.gene49174:CDS 60.0%
! TCTAAGGGGGGCTCTGATGG+TGG + chr1.3:68488250-68488269 None:intergenic 60.0%
CCAGGGTGGGATGCAAGGCA+CGG - chr1.3:68488488-68488507 MS.gene49174:CDS 65.0%
!! GGCTCTAAGGGGGGCTCTGA+TGG + chr1.3:68488253-68488272 None:intergenic 65.0%
CCGTGCCTTGCATCCCACCC+TGG + chr1.3:68488491-68488510 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 68488112 68490178 68488112 ID=MS.gene49174
chr1.3 mRNA 68488112 68490178 68488112 ID=MS.gene49174.t1;Parent=MS.gene49174
chr1.3 exon 68489581 68490178 68489581 ID=MS.gene49174.t1.exon1;Parent=MS.gene49174.t1
chr1.3 CDS 68489581 68490178 68489581 ID=cds.MS.gene49174.t1;Parent=MS.gene49174.t1
chr1.3 exon 68488112 68488974 68488112 ID=MS.gene49174.t1.exon2;Parent=MS.gene49174.t1
chr1.3 CDS 68488112 68488974 68488112 ID=cds.MS.gene49174.t1;Parent=MS.gene49174.t1
Gene Sequence

>MS.gene49174

ATGTTTAAAAGTCGCAATACTGTTTGCGTTATTATGGCAGGTCATGAGATGACAGATATGGATGGAGATTTTACCATGGTTGTCTCAAATGATACACATGTCAGTGCTCTTGATGTGAAGCCTCTCAATAGTGAACCACCATCAGAGCCCCCCTTAGAGCCCATTGAAGGAATGGAGTTTAAATCATTTGAAGAAGCAAAGAGTTACTACACAAGGTACGCTCAGAACAAAGGTTTTAGTTTCCGCATGGGCCGTGTTACTAAGTCAAGAACAAACGGTATGATAATTGGTCAAGAAATTCTTTGTTCCAAGGAGGGGTTTCGAGCCAAAAAGTATGTGAAACAAGGAAATAGTAGTCTTATTACACATGATGAAACCAGGGTGGGATGCAAGGCACGGTTATATTTAAAGAAAAATAATGATATATGGATTGTTTCTAGATTTGTAAGTGACCATAATCACCAGCTGTTTTCTCCTAGAAGTGCACAGTCTCTTCGCGTACATAGAAAGAAAACTAAAGTGCAAAAGACACTTACTGATGTGCTTGATGAATCTGGTTTAGGCAAAACAACATCTGTCTTATGCACTGAAAGTGGCGGTATTGATAATTTTGGATCTAGCCAACAAGATGTTATCAATTATTTAAGTGTACAGAGACAAAAACAATTTGAAAACAGAGATGCTCAATTGATGTTATCATACTTTAAGAATTGTCAGTTGAAGAATACTGGGTTTTTCTATGCGTTTCAAATGGATGCCGAAGGAAAATTAACTAATTGCTTTTGGGTTGATTCAAGGTCTAGAGTTGCTTACAAATATTTTGGTGATGTGGTTACTTTTGATCCAACATACTTGAAAAATAAATACAAAACTCCTTTTGTTCCAATTACTGGAGTTAATCATCATCAACAATCAATTCTTTTTGGTTGTGCTCTCTTGTGGGATGAAACTGTAGAGAGTTTTGTTTGGTTGCTAAGTACTTGGTTAGAAGCAATGAATGGGGTTTGCCCTAAAACTATTATCACAGACCAACACACTGCTATCACCAATGCAGTTGCAAGGGTGTTTCCAAAGGTTAATCACCATTATTGCATGTGGCATATTGAGGAAAAAGTTCCAGAGCACATGAATCAGATCTATCGTGGACACAGTGAATTTAAAAATCATTTTTACAAGTGCATTCACCAATCTATCACAATTGAAGAATTTGAGTCTGAATGGGAAGCAATGATTGATAAATACGGGTTGCAAGATAATCAGTGGCTAGTGAAGATATACTCAATTCGATCAAAATGGATTCCTGCATATGTACATCATAATTTTTGTGCTGGAATGTCTACCACTCATAGGAGTGAAAGTATGAATAAGTTTTTTAAGGATTTCTTGAATTCAAGTACTCCATTGAGCAAGTTTCTGACACAATATGAGAAAGCTCTTGATGCACGTTACAACGAGGAAAGGGAGAAAACTGTCAAGACGATGAATTCAAAACCACTTTTGCGAACTTTATACCCCATGGAAGAAGAAGCTTCAAAAATTTATACCGGAAAATTGTTTGAGATATTTCAAGATGAGTTGGTTGGCTCTCAAATGTTTACCACAGAAAAAGTTGAATTTTCTGATGAAGTGGCAACATACAAAGTTCATGAAATTTACAAAGAGAAGCCTAAATATCATGTGACTTTTCATGTAACTTCAAAAGAAGCAACTTGTACTTGTCAAAAGTTTGAATCGTTTGGTATTCTTTGTAGGCATATCTTAACTGTGTTCCTAAAGAAAAAGGTACGTTATCTCCCTTCACAGTATGTTCTACAACGATGGACCCAAAATGCTAAGAAAGAAAAATTTGAAGGATTGACAATTGAAGAATTTGAAGGAGGAAACAAAGCTTCTAGCACTTCATTGTTTAATAGTGTTATGGTTCGTTCACTGGAATTTTCTGAAAGAGCTTCATGGTCAAAAAAACATCATGATATTGCAATTCAATGTTTGCAAAATGCAATTTCAAAACTTGATCTATTAGAGCTTGAAGAATCAAATGAGGAGTTTGTTAATTTGACATCTTAA
Protein sequence

>MS.gene49174.t1

MFKSRNTVCVIMAGHEMTDMDGDFTMVVSNDTHVSALDVKPLNSEPPSEPPLEPIEGMEFKSFEEAKSYYTRYAQNKGFSFRMGRVTKSRTNGMIIGQEILCSKEGFRAKKYVKQGNSSLITHDETRVGCKARLYLKKNNDIWIVSRFVSDHNHQLFSPRSAQSLRVHRKKTKVQKTLTDVLDESGLGKTTSVLCTESGEFESEWEAMIDKYGLQDNQWLVKIYSIRSKWIPAYVHHNFCAGMSTTHRSESMNKFFKDFLNSSTPLSKFLTQYEKALDARYNEEREKTVKTMNSKPLLRTLYPMEEEASKIYTGKLFEIFQDELVGSQMFTTEKVEFSDEVATYKVHEIYKEKPKYHVTFHVTSKEATCTCQKFESFGILCRHILTVFLKKKVRYLPSQYVLQRWTQNAKKEKFEGLTIEEFEGGNKASSTSLFNSVMVRSLEFSERASWSKKHHDIAIQCLQNAISKLDLLELEESNEEFVNLTS