AlfalfaGEDB | MS.gene49174

M. sativa cultivar XinJiangDaYe / MS.gene49174

KEGG: -
GO: GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
PFAM: PF03101.18 PF04434.20

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49174.t1	RHN81732.1	67.6	675	16	2	15	486	12	686	7.70E-236	826.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49174.t1	Q9SZL8	27.4	638	256	6	52	483	71	707	8.5e-58	226.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49174.t1	A0A396JW00	67.6	675	16	2	15	486	12	686	5.6e-236	826.2

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene49174.t1	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49174.t1	MTR_6g053640	55.645	124	53	2	364	485	2	125	1.87e-34	126
MS.gene49174.t1	MTR_3g005220	59.406	101	37	2	8	107	28	125	8.33e-32	120
MS.gene49174.t1	MTR_6g024175	65.854	82	27	1	385	465	103	184	2.11e-26	106
MS.gene49174.t1	MTR_2g098880	44.954	109	59	1	52	159	59	167	7.37e-25	103
MS.gene49174.t1	MTR_2g098880	44.954	109	59	1	52	159	71	179	1.05e-24	103
MS.gene49174.t1	MTR_2g098920	38.596	114	58	3	53	161	79	185	6.92e-19	86.7
MS.gene49174.t1	MTR_4g067290	33.582	134	77	3	28	156	30	156	2.83e-18	84.3
MS.gene49174.t1	MTR_4g067290	33.582	134	77	3	28	156	30	156	2.83e-18	84.3
MS.gene49174.t1	MTR_4g067290	33.582	134	77	3	28	156	50	176	4.05e-18	84.3
MS.gene49174.t1	MTR_4g067290	33.582	134	77	3	28	156	50	176	4.19e-18	84.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49174.t1	AT4G38180	42.294	279	156	5	196	471	419	695	1.73e-60	212
MS.gene49174.t1	AT4G38180	41.447	152	85	3	52	199	71	222	6.83e-24	105
MS.gene49174.t1	AT4G38170	33.824	272	178	1	198	469	187	456	1.71e-48	175
MS.gene49174.t1	AT2G27110	33.455	275	174	4	200	472	226	493	1.80e-47	175
MS.gene49174.t1	AT2G27110	33.455	275	174	4	200	472	371	638	3.90e-47	175
MS.gene49174.t1	AT2G27110	33.455	275	174	4	200	472	371	638	3.90e-47	175
MS.gene49174.t1	AT5G18960	41.232	211	121	1	200	410	521	728	3.30e-45	169
MS.gene49174.t1	AT5G18960	35.238	105	52	2	53	156	210	299	5.45e-13	72.0
MS.gene49174.t1	AT5G18960	32.075	106	56	2	52	156	41	131	1.27e-12	70.9
MS.gene49174.t1	AT5G18960	41.232	211	121	1	200	410	521	728	3.30e-45	169

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Find 70 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTATGATGTACATATGC+AGG	0.504291	1.3:+68488857	None:intergenic
AAATTCTTTGTTCCAAGGAG+GGG	0.533751	1.3:-68489861	MS.gene49174:CDS
AACTGTGTTCCTAAAGAAAA+AGG	0.312218	1.3:-68488396	MS.gene49174:CDS
AAGCAAAGAGTTACTACACA+AGG	0.651539	1.3:-68489963	MS.gene49174:CDS
AAGCAATGATTGATAAATAC+GGG	0.513517	1.3:-68488934	MS.gene49174:CDS
AATCGTTTGGTATTCTTTGT+AGG	0.315386	1.3:-68488427	MS.gene49174:CDS
ACAAGGTACGCTCAGAACAA+AGG	0.639870	1.3:-68489946	MS.gene49174:CDS
ACAATTGAAGAATTTGAAGG+AGG	0.530417	1.3:-68488299	MS.gene49174:CDS
ACACATGATGAAACCAGGGT+GGG	0.653979	1.3:-68489793	MS.gene49174:CDS
ACGGGTTGCAAGATAATCAG+TGG	0.648204	1.3:-68488916	MS.gene49174:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTGTGTTCCTAAAGAAAA+AGG	-	chr1.3:68489872-68489891	MS.gene49174:CDS	30.0%
AATCGTTTGGTATTCTTTGT+AGG	-	chr1.3:68489841-68489860	MS.gene49174:CDS	30.0%
AATTGTCAGTTGAAGAATAC+TGG	-	chr1.3:68488820-68488839	MS.gene49174:CDS	30.0%
ACAATTGAAGAATTTGAAGG+AGG	-	chr1.3:68489969-68489988	MS.gene49174:CDS	30.0%
AGAACAAACGGTATGATAAT+TGG	-	chr1.3:68488379-68488398	MS.gene49174:CDS	30.0%
ATTGTCAGTTGAAGAATACT+GGG	-	chr1.3:68488821-68488840	MS.gene49174:CDS	30.0%
CAATAATGGTGATTAACCTT+TGG	+	chr1.3:68489183-68489202	None:intergenic	30.0%
CAATACTGTTTGCGTTATTA+TGG	-	chr1.3:68488126-68488145	MS.gene49174:CDS	30.0%
GTTACTAAGTCAAGAACAAA+CGG	-	chr1.3:68488367-68488386	MS.gene49174:CDS	30.0%
TATTACACATGATGAAACCA+GGG	-	chr1.3:68488471-68488490	MS.gene49174:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	68488112	68490178	68488112	ID=MS.gene49174
chr1.3	mRNA	68488112	68490178	68488112	ID=MS.gene49174.t1;Parent=MS.gene49174
chr1.3	exon	68489581	68490178	68489581	ID=MS.gene49174.t1.exon1;Parent=MS.gene49174.t1
chr1.3	CDS	68489581	68490178	68489581	ID=cds.MS.gene49174.t1;Parent=MS.gene49174.t1
chr1.3	exon	68488112	68488974	68488112	ID=MS.gene49174.t1.exon2;Parent=MS.gene49174.t1
chr1.3	CDS	68488112	68488974	68488112	ID=cds.MS.gene49174.t1;Parent=MS.gene49174.t1

Gene Sequence

>MS.gene49174

ATGTTTAAAAGTCGCAATACTGTTTGCGTTATTATGGCAGGTCATGAGATGACAGATATGGATGGAGATTTTACCATGGTTGTCTCAAATGATACACATGTCAGTGCTCTTGATGTGAAGCCTCTCAATAGTGAACCACCATCAGAGCCCCCCTTAGAGCCCATTGAAGGAATGGAGTTTAAATCATTTGAAGAAGCAAAGAGTTACTACACAAGGTACGCTCAGAACAAAGGTTTTAGTTTCCGCATGGGCCGTGTTACTAAGTCAAGAACAAACGGTATGATAATTGGTCAAGAAATTCTTTGTTCCAAGGAGGGGTTTCGAGCCAAAAAGTATGTGAAACAAGGAAATAGTAGTCTTATTACACATGATGAAACCAGGGTGGGATGCAAGGCACGGTTATATTTAAAGAAAAATAATGATATATGGATTGTTTCTAGATTTGTAAGTGACCATAATCACCAGCTGTTTTCTCCTAGAAGTGCACAGTCTCTTCGCGTACATAGAAAGAAAACTAAAGTGCAAAAGACACTTACTGATGTGCTTGATGAATCTGGTTTAGGCAAAACAACATCTGTCTTATGCACTGAAAGTGGCGGTATTGATAATTTTGGATCTAGCCAACAAGATGTTATCAATTATTTAAGTGTACAGAGACAAAAACAATTTGAAAACAGAGATGCTCAATTGATGTTATCATACTTTAAGAATTGTCAGTTGAAGAATACTGGGTTTTTCTATGCGTTTCAAATGGATGCCGAAGGAAAATTAACTAATTGCTTTTGGGTTGATTCAAGGTCTAGAGTTGCTTACAAATATTTTGGTGATGTGGTTACTTTTGATCCAACATACTTGAAAAATAAATACAAAACTCCTTTTGTTCCAATTACTGGAGTTAATCATCATCAACAATCAATTCTTTTTGGTTGTGCTCTCTTGTGGGATGAAACTGTAGAGAGTTTTGTTTGGTTGCTAAGTACTTGGTTAGAAGCAATGAATGGGGTTTGCCCTAAAACTATTATCACAGACCAACACACTGCTATCACCAATGCAGTTGCAAGGGTGTTTCCAAAGGTTAATCACCATTATTGCATGTGGCATATTGAGGAAAAAGTTCCAGAGCACATGAATCAGATCTATCGTGGACACAGTGAATTTAAAAATCATTTTTACAAGTGCATTCACCAATCTATCACAATTGAAGAATTTGAGTCTGAATGGGAAGCAATGATTGATAAATACGGGTTGCAAGATAATCAGTGGCTAGTGAAGATATACTCAATTCGATCAAAATGGATTCCTGCATATGTACATCATAATTTTTGTGCTGGAATGTCTACCACTCATAGGAGTGAAAGTATGAATAAGTTTTTTAAGGATTTCTTGAATTCAAGTACTCCATTGAGCAAGTTTCTGACACAATATGAGAAAGCTCTTGATGCACGTTACAACGAGGAAAGGGAGAAAACTGTCAAGACGATGAATTCAAAACCACTTTTGCGAACTTTATACCCCATGGAAGAAGAAGCTTCAAAAATTTATACCGGAAAATTGTTTGAGATATTTCAAGATGAGTTGGTTGGCTCTCAAATGTTTACCACAGAAAAAGTTGAATTTTCTGATGAAGTGGCAACATACAAAGTTCATGAAATTTACAAAGAGAAGCCTAAATATCATGTGACTTTTCATGTAACTTCAAAAGAAGCAACTTGTACTTGTCAAAAGTTTGAATCGTTTGGTATTCTTTGTAGGCATATCTTAACTGTGTTCCTAAAGAAAAAGGTACGTTATCTCCCTTCACAGTATGTTCTACAACGATGGACCCAAAATGCTAAGAAAGAAAAATTTGAAGGATTGACAATTGAAGAATTTGAAGGAGGAAACAAAGCTTCTAGCACTTCATTGTTTAATAGTGTTATGGTTCGTTCACTGGAATTTTCTGAAAGAGCTTCATGGTCAAAAAAACATCATGATATTGCAATTCAATGTTTGCAAAATGCAATTTCAAAACTTGATCTATTAGAGCTTGAAGAATCAAATGAGGAGTTTGTTAATTTGACATCTTAA

Protein sequence

>MS.gene49174.t1

MFKSRNTVCVIMAGHEMTDMDGDFTMVVSNDTHVSALDVKPLNSEPPSEPPLEPIEGMEFKSFEEAKSYYTRYAQNKGFSFRMGRVTKSRTNGMIIGQEILCSKEGFRAKKYVKQGNSSLITHDETRVGCKARLYLKKNNDIWIVSRFVSDHNHQLFSPRSAQSLRVHRKKTKVQKTLTDVLDESGLGKTTSVLCTESGEFESEWEAMIDKYGLQDNQWLVKIYSIRSKWIPAYVHHNFCAGMSTTHRSESMNKFFKDFLNSSTPLSKFLTQYEKALDARYNEEREKTVKTMNSKPLLRTLYPMEEEASKIYTGKLFEIFQDELVGSQMFTTEKVEFSDEVATYKVHEIYKEKPKYHVTFHVTSKEATCTCQKFESFGILCRHILTVFLKKKVRYLPSQYVLQRWTQNAKKEKFEGLTIEEFEGGNKASSTSLFNSVMVRSLEFSERASWSKKHHDIAIQCLQNAISKLDLLELEESNEEFVNLTS