Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49212.t1 | XP_024636348.1 | 98.7 | 155 | 2 | 0 | 1 | 155 | 1 | 155 | 4.00E-83 | 317.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49212.t1 | Q9SZL8 | 33.3 | 153 | 97 | 3 | 20 | 168 | 76 | 227 | 5.8e-14 | 79.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49212.t1 | A0A396GJD5 | 76.2 | 164 | 39 | 0 | 1 | 164 | 1 | 164 | 6.5e-67 | 263.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene49212.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49212.t1 | MTR_6g051425 | 70.930 | 172 | 46 | 1 | 1 | 168 | 1 | 172 | 1.13e-87 | 260 |
| MS.gene49212.t1 | MTR_2g039370 | 82.432 | 74 | 13 | 0 | 49 | 122 | 98 | 171 | 5.65e-38 | 132 |
| MS.gene49212.t1 | MTR_3g465760 | 73.913 | 69 | 14 | 1 | 41 | 105 | 2 | 70 | 1.24e-29 | 103 |
| MS.gene49212.t1 | MTR_2g098880 | 30.579 | 121 | 74 | 3 | 7 | 121 | 51 | 167 | 1.64e-13 | 66.6 |
| MS.gene49212.t1 | MTR_2g098880 | 30.579 | 121 | 74 | 3 | 7 | 121 | 63 | 179 | 2.17e-13 | 66.2 |
| MS.gene49212.t1 | MTR_2g098920 | 29.126 | 103 | 72 | 1 | 18 | 120 | 81 | 182 | 3.77e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49212.t1 | AT4G38180 | 34.228 | 149 | 93 | 3 | 20 | 164 | 76 | 223 | 1.71e-17 | 79.3 |
| MS.gene49212.t1 | AT3G59470 | 32.039 | 103 | 69 | 1 | 18 | 120 | 71 | 172 | 1.06e-12 | 64.3 |
| MS.gene49212.t1 | AT3G59470 | 32.039 | 103 | 69 | 1 | 18 | 120 | 71 | 172 | 1.06e-12 | 64.3 |
| MS.gene49212.t1 | AT3G59470 | 32.039 | 103 | 69 | 1 | 18 | 120 | 83 | 184 | 1.14e-12 | 64.3 |
| MS.gene49212.t1 | AT5G28530 | 28.477 | 151 | 101 | 2 | 18 | 163 | 56 | 204 | 7.69e-12 | 62.8 |
| MS.gene49212.t1 | AT5G28530 | 28.477 | 151 | 101 | 2 | 18 | 163 | 56 | 204 | 7.90e-12 | 62.8 |
| MS.gene49212.t1 | AT5G28530 | 28.477 | 151 | 101 | 2 | 18 | 163 | 56 | 204 | 3.38e-11 | 61.2 |
| MS.gene49212.t1 | AT5G28530 | 28.477 | 151 | 101 | 2 | 18 | 163 | 56 | 204 | 3.38e-11 | 61.2 |
| MS.gene49212.t1 | AT5G28530 | 28.477 | 151 | 101 | 2 | 18 | 163 | 56 | 204 | 3.59e-11 | 60.8 |
Find 34 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAATATTTGGAATTATTT+AGG | 0.090273 | 3.1:-73579821 | MS.gene49212:CDS |
| GTATACGAGTTACAAATATT+TGG | 0.221426 | 3.1:-73579832 | MS.gene49212:CDS |
| CAAATATTTGGAATTATTTA+GGG | 0.245589 | 3.1:-73579820 | MS.gene49212:CDS |
| ATTATTCTTAAACCCTTCTT+TGG | 0.257903 | 3.1:+73580075 | None:intergenic |
| ATGTTAGCAAGGAAGGAGTT+TGG | 0.356508 | 3.1:-73579982 | MS.gene49212:CDS |
| GGGTTTAAGAATAATGAATA+TGG | 0.371754 | 3.1:-73580067 | MS.gene49212:CDS |
| AGGAGACCTCATAAAGTCTA+TGG | 0.381794 | 3.1:-73579864 | MS.gene49212:CDS |
| AACAGTAAAACTCTTTAAAA+CGG | 0.389800 | 3.1:+73580098 | None:intergenic |
| CAAGCACATGCCTTCAAAAC+AGG | 0.394361 | 3.1:-73580175 | MS.gene49212:CDS |
| CAGGATTTAGTGTAAGAAAA+GGG | 0.432600 | 3.1:-73580156 | MS.gene49212:CDS |
| TGTCAATCAGTTGAAATTGA+TGG | 0.438803 | 3.1:-73580259 | MS.gene49212:CDS |
| ACAGGATTTAGTGTAAGAAA+AGG | 0.440176 | 3.1:-73580157 | MS.gene49212:CDS |
| AGGTTTAATGTTAGCAAGGA+AGG | 0.442161 | 3.1:-73579989 | MS.gene49212:CDS |
| ATAAAGTCTATGGTGAATTC+TGG | 0.445432 | 3.1:-73579854 | MS.gene49212:CDS |
| GACATTTGACATATTTCTCA+TGG | 0.451386 | 3.1:+73579889 | None:intergenic |
| ACAGTAAAACTCTTTAAAAC+GGG | 0.452807 | 3.1:+73580099 | None:intergenic |
| AATTCACCATAGACTTTATG+AGG | 0.457499 | 3.1:+73579858 | None:intergenic |
| GTCGGTGGATATAACAATCT+TGG | 0.477476 | 3.1:-73579791 | None:intergenic |
| CTTATGAAAGAGCTGATTCA+AGG | 0.480001 | 3.1:-73580033 | MS.gene49212:CDS |
| CGCAACAAATGTCTTTGCTC+AGG | 0.482031 | 3.1:+73579914 | None:intergenic |
| TTGGAATTATTTAGGGGAAG+AGG | 0.485109 | 3.1:-73579813 | MS.gene49212:CDS |
| AAGGACAAATTGCAAAGCTA+TGG | 0.528165 | 3.1:-73580014 | MS.gene49212:CDS |
| AGAAATATGTCAAATGTCAA+AGG | 0.545496 | 3.1:-73579884 | MS.gene49212:CDS |
| ATTTAGTGTAAGAAAAGGGA+GGG | 0.556280 | 3.1:-73580152 | MS.gene49212:CDS |
| GATTTAGTGTAAGAAAAGGG+AGG | 0.567737 | 3.1:-73580153 | MS.gene49212:CDS |
| TAATGAATATGGAGGTGAAG+TGG | 0.572461 | 3.1:-73580056 | MS.gene49212:CDS |
| GGTGAGGTTTAATGTTAGCA+AGG | 0.579640 | 3.1:-73579993 | MS.gene49212:CDS |
| TATTTAGGGGAAGAGGTCGG+TGG | 0.586341 | 3.1:-73579806 | MS.gene49212:CDS |
| AATTATTTAGGGGAAGAGGT+CGG | 0.592715 | 3.1:-73579809 | MS.gene49212:CDS |
| TTTAAGAATAATGAATATGG+AGG | 0.604513 | 3.1:-73580064 | MS.gene49212:CDS |
| CTGAGCAAAGACATTTGTTG+CGG | 0.611498 | 3.1:-73579913 | MS.gene49212:CDS |
| CAAATTGCAAAGCTATGGTG+AGG | 0.629755 | 3.1:-73580009 | MS.gene49212:CDS |
| AAATATTTGGAATTATTTAG+GGG | 0.632966 | 3.1:-73579819 | MS.gene49212:CDS |
| AACAAATGTCTTTGCTCAGG+AGG | 0.641459 | 3.1:+73579917 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATTTGGAATTATTTAG+GGG | - | chr3.1:73580247-73580266 | MS.gene49212:CDS | 15.0% |
| !!! | ACAAATATTTGGAATTATTT+AGG | - | chr3.1:73580245-73580264 | MS.gene49212:CDS | 15.0% |
| !!! | CAAATATTTGGAATTATTTA+GGG | - | chr3.1:73580246-73580265 | MS.gene49212:CDS | 15.0% |
| !! | TTTAAGAATAATGAATATGG+AGG | - | chr3.1:73580002-73580021 | MS.gene49212:CDS | 20.0% |
| !!! | AACAGTAAAACTCTTTAAAA+CGG | + | chr3.1:73579971-73579990 | None:intergenic | 20.0% |
| ! | AGAAATATGTCAAATGTCAA+AGG | - | chr3.1:73580182-73580201 | MS.gene49212:CDS | 25.0% |
| ! | ATTATTCTTAAACCCTTCTT+TGG | + | chr3.1:73579994-73580013 | None:intergenic | 25.0% |
| ! | GGGTTTAAGAATAATGAATA+TGG | - | chr3.1:73579999-73580018 | MS.gene49212:CDS | 25.0% |
| ! | GTATACGAGTTACAAATATT+TGG | - | chr3.1:73580234-73580253 | MS.gene49212:CDS | 25.0% |
| !!! | ACAGTAAAACTCTTTAAAAC+GGG | + | chr3.1:73579970-73579989 | None:intergenic | 25.0% |
| AATTCACCATAGACTTTATG+AGG | + | chr3.1:73580211-73580230 | None:intergenic | 30.0% | |
| ACAGGATTTAGTGTAAGAAA+AGG | - | chr3.1:73579909-73579928 | MS.gene49212:CDS | 30.0% | |
| ATAAAGTCTATGGTGAATTC+TGG | - | chr3.1:73580212-73580231 | MS.gene49212:CDS | 30.0% | |
| ATTTAGTGTAAGAAAAGGGA+GGG | - | chr3.1:73579914-73579933 | MS.gene49212:CDS | 30.0% | |
| CAGGATTTAGTGTAAGAAAA+GGG | - | chr3.1:73579910-73579929 | MS.gene49212:CDS | 30.0% | |
| GACATTTGACATATTTCTCA+TGG | + | chr3.1:73580180-73580199 | None:intergenic | 30.0% | |
| TGTCAATCAGTTGAAATTGA+TGG | - | chr3.1:73579807-73579826 | MS.gene49212:CDS | 30.0% | |
| ! | AGTTTTACTGTTCCAAAGAA+GGG | - | chr3.1:73579979-73579998 | MS.gene49212:CDS | 30.0% |
| ! | GGATGCAAAGATATTTTTCT+TGG | - | chr3.1:73579828-73579847 | MS.gene49212:CDS | 30.0% |
| !! | TACACTAAATCCTGTTTTGA+AGG | + | chr3.1:73579904-73579923 | None:intergenic | 30.0% |
| AAGGACAAATTGCAAAGCTA+TGG | - | chr3.1:73580052-73580071 | MS.gene49212:CDS | 35.0% | |
| AATTATTTAGGGGAAGAGGT+CGG | - | chr3.1:73580257-73580276 | MS.gene49212:CDS | 35.0% | |
| AGGTTTAATGTTAGCAAGGA+AGG | - | chr3.1:73580077-73580096 | MS.gene49212:CDS | 35.0% | |
| GATTTAGTGTAAGAAAAGGG+AGG | - | chr3.1:73579913-73579932 | MS.gene49212:CDS | 35.0% | |
| TAATGAATATGGAGGTGAAG+TGG | - | chr3.1:73580010-73580029 | MS.gene49212:CDS | 35.0% | |
| TTGGAATTATTTAGGGGAAG+AGG | - | chr3.1:73580253-73580272 | MS.gene49212:CDS | 35.0% | |
| ! | CTTATGAAAGAGCTGATTCA+AGG | - | chr3.1:73580033-73580052 | MS.gene49212:CDS | 35.0% |
| ! | GAGTTTTACTGTTCCAAAGA+AGG | - | chr3.1:73579978-73579997 | MS.gene49212:CDS | 35.0% |
| AACAAATGTCTTTGCTCAGG+AGG | + | chr3.1:73580152-73580171 | None:intergenic | 40.0% | |
| AGGAGACCTCATAAAGTCTA+TGG | - | chr3.1:73580202-73580221 | MS.gene49212:CDS | 40.0% | |
| ATGTTAGCAAGGAAGGAGTT+TGG | - | chr3.1:73580084-73580103 | MS.gene49212:CDS | 40.0% | |
| CAAATTGCAAAGCTATGGTG+AGG | - | chr3.1:73580057-73580076 | MS.gene49212:CDS | 40.0% | |
| GGTGAGGTTTAATGTTAGCA+AGG | - | chr3.1:73580073-73580092 | MS.gene49212:CDS | 40.0% | |
| ! | CTGAGCAAAGACATTTGTTG+CGG | - | chr3.1:73580153-73580172 | MS.gene49212:CDS | 40.0% |
| CAAGCACATGCCTTCAAAAC+AGG | - | chr3.1:73579891-73579910 | MS.gene49212:CDS | 45.0% | |
| CGCAACAAATGTCTTTGCTC+AGG | + | chr3.1:73580155-73580174 | None:intergenic | 45.0% | |
| TATTTAGGGGAAGAGGTCGG+TGG | - | chr3.1:73580260-73580279 | MS.gene49212:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 73579792 | 73580296 | 73579792 | ID=MS.gene49212 |
| chr3.1 | mRNA | 73579792 | 73580296 | 73579792 | ID=MS.gene49212.t1;Parent=MS.gene49212 |
| chr3.1 | exon | 73579792 | 73580296 | 73579792 | ID=MS.gene49212.t1.exon1;Parent=MS.gene49212.t1 |
| chr3.1 | CDS | 73579792 | 73580296 | 73579792 | ID=cds.MS.gene49212.t1;Parent=MS.gene49212.t1 |
| Gene Sequence |
| Protein sequence |
