AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49318

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49318.t1 MTR_1g070220 92.271 207 13 3 1 204 1 207 1.25e-138 386
MS.gene49318.t1 MTR_7g096530 59.459 222 66 6 1 202 1 218 2.71e-79 236
MS.gene49318.t1 MTR_7g096610 55.414 157 60 2 12 168 5 151 3.32e-49 160
MS.gene49318.t1 MTR_8g036105 64.103 117 42 0 6 122 9 125 5.14e-48 156
MS.gene49318.t1 MTR_1g070205 70.297 101 30 0 15 115 12 112 1.06e-47 156
MS.gene49318.t1 MTR_6g027710 68.317 101 32 0 15 115 40 140 5.38e-46 152
MS.gene49318.t1 MTR_8g036085 72.632 95 26 0 15 109 23 117 1.09e-45 151
MS.gene49318.t1 MTR_7g074990 63.366 101 37 0 15 115 20 120 1.94e-42 143
MS.gene49318.t1 MTR_7g075230 52.113 142 63 3 13 149 10 151 3.90e-42 141
MS.gene49318.t1 MTR_5g080470 62.887 97 36 0 13 109 5 101 4.06e-42 140
MS.gene49318.t1 MTR_4g083680 52.137 117 55 1 1 117 1 116 4.32e-40 135
MS.gene49318.t1 MTR_2g068760 53.097 113 52 1 5 117 3 114 3.63e-39 132
MS.gene49318.t1 MTR_6g027700 60.784 102 40 0 15 116 7 108 4.74e-38 130
MS.gene49318.t1 MTR_3g031660 48.837 129 65 1 11 139 3 130 9.80e-38 129
MS.gene49318.t1 MTR_5g083230 59.406 101 40 1 15 115 6 105 1.12e-37 128
MS.gene49318.t1 MTR_7g033800 54.472 123 55 1 7 129 3 124 1.13e-37 129
MS.gene49318.t1 MTR_6g005070 49.640 139 63 4 12 150 6 137 1.04e-36 125
MS.gene49318.t1 MTR_3g452660 59.048 105 42 1 11 115 3 106 3.71e-36 128
MS.gene49318.t1 MTR_3g071590 52.542 118 54 2 15 131 8 124 7.77e-36 124
MS.gene49318.t1 MTR_5g017950 58.095 105 43 1 11 115 4 107 1.96e-35 127
MS.gene49318.t1 MTR_4g105170 62.105 95 35 1 15 109 11 104 2.09e-35 123
MS.gene49318.t1 MTR_3g077240 57.407 108 45 1 7 114 2 108 2.66e-35 123
MS.gene49318.t1 MTR_6g005080 55.660 106 46 1 12 117 7 111 1.23e-34 120
MS.gene49318.t1 MTR_5g083960 53.465 101 46 1 15 115 39 138 4.43e-34 120
MS.gene49318.t1 MTR_3g094690 52.679 112 51 2 15 126 47 156 9.31e-34 120
MS.gene49318.t1 MTR_3g071420 50.442 113 54 2 1 113 1 111 4.66e-33 117
MS.gene49318.t1 MTR_3g073690 56.436 101 43 1 15 115 9 108 5.90e-33 117
MS.gene49318.t1 MTR_2g093310 47.107 121 62 2 8 128 26 144 2.75e-32 115
MS.gene49318.t1 MTR_3g094690 52.679 112 50 3 15 126 47 155 5.76e-32 116
MS.gene49318.t1 MTR_5g083010 51.852 108 50 2 6 113 3 108 7.64e-32 114
MS.gene49318.t1 MTR_4g060950 47.200 125 63 3 2 125 11 133 2.94e-31 112
MS.gene49318.t1 MTR_8g040900 62.195 82 30 1 11 92 3 83 6.80e-31 113
MS.gene49318.t1 MTR_5g075020 51.261 119 57 1 15 133 43 160 9.54e-30 110
MS.gene49318.t1 MTR_5g075020 51.261 119 57 1 15 133 47 164 1.64e-29 109
MS.gene49318.t1 MTR_1g095850 50.980 102 49 1 16 117 73 173 2.31e-29 109
MS.gene49318.t1 MTR_5g093010 48.000 100 51 1 16 115 6 104 5.77e-27 100
MS.gene49318.t1 MTR_7g028905 46.000 100 53 1 16 115 6 104 2.44e-26 98.2
MS.gene49318.t1 MTR_8g079660 44.554 101 55 1 15 115 8 107 5.26e-23 93.2
MS.gene49318.t1 MTR_8g079620 41.414 99 57 1 15 113 8 105 8.85e-20 84.3
MS.gene49318.t1 MTR_4g107450 43.000 100 56 1 16 115 11 109 1.36e-19 84.7
MS.gene49318.t1 MTR_2g100020 39.000 100 60 1 16 115 20 118 1.98e-19 84.0
MS.gene49318.t1 MTR_6g011250 45.679 81 42 2 16 95 10 89 3.29e-18 79.0
MS.gene49318.t1 MTR_6g011230 37.755 98 60 1 16 113 12 108 5.42e-17 75.9
MS.gene49318.t1 MTR_6g011200 36.364 99 61 2 16 113 10 107 1.69e-16 75.5
MS.gene49318.t1 MTR_8g079580 33.333 144 77 5 15 152 8 138 2.16e-16 75.1
MS.gene49318.t1 MTR_8g017090 37.864 103 63 1 16 118 13 114 1.13e-15 73.9
MS.gene49318.t1 MTR_4g099230 38.542 96 53 4 16 106 3 97 7.95e-14 66.6
MS.gene49318.t1 MTR_5g085390 37.000 100 61 2 16 114 10 108 1.33e-12 64.7
MS.gene49318.t1 MTR_4g088035 30.833 120 82 1 16 135 7 125 4.96e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49318.t1 AT2G42430 47.154 246 85 7 1 202 1 245 4.75e-63 196
MS.gene49318.t1 AT3G58190 60.317 126 48 1 15 138 11 136 2.82e-48 157
MS.gene49318.t1 AT3G03760 70.755 106 31 0 15 120 51 156 2.84e-48 159
MS.gene49318.t1 AT2G42440 61.345 119 46 0 12 130 4 122 1.54e-47 156
MS.gene49318.t1 AT2G31310 65.049 103 36 0 13 115 5 107 1.34e-46 152
MS.gene49318.t1 AT2G45420 70.408 98 29 0 18 115 40 137 1.45e-44 149
MS.gene49318.t1 AT4G00220 68.367 98 31 0 18 115 20 117 2.64e-44 147
MS.gene49318.t1 AT4G00210 60.909 110 43 0 13 122 9 118 3.87e-44 147
MS.gene49318.t1 AT5G06080 60.952 105 41 0 11 115 3 107 3.73e-43 143
MS.gene49318.t1 AT2G45410 68.367 98 31 0 18 115 19 116 3.81e-43 143
MS.gene49318.t1 AT3G27650 60.000 100 39 1 15 114 39 137 4.00e-38 129
MS.gene49318.t1 AT5G63090 60.194 103 40 1 7 109 3 104 5.44e-36 125
MS.gene49318.t1 AT5G63090 60.194 103 40 1 7 109 3 104 5.44e-36 125
MS.gene49318.t1 AT5G63090 60.194 103 40 1 7 109 3 104 5.44e-36 125
MS.gene49318.t1 AT5G63090 60.194 103 40 1 7 109 3 104 5.44e-36 125
MS.gene49318.t1 AT2G30130 58.416 101 41 1 15 115 8 107 1.98e-35 124
MS.gene49318.t1 AT1G31320 55.208 96 42 1 7 102 5 99 6.21e-34 119
MS.gene49318.t1 AT1G07900 52.427 103 48 1 15 117 33 134 1.65e-33 119
MS.gene49318.t1 AT5G66870 55.238 105 46 1 11 115 3 106 3.08e-33 121
MS.gene49318.t1 AT2G30340 50.450 111 54 1 15 125 53 162 3.21e-33 120
MS.gene49318.t1 AT2G30340 47.934 121 60 2 15 133 52 171 3.31e-33 120
MS.gene49318.t1 AT1G16530 46.774 124 64 2 15 137 14 136 8.92e-33 116
MS.gene49318.t1 AT2G28500 48.696 115 57 2 15 129 55 167 1.25e-32 117
MS.gene49318.t1 AT2G23660 51.923 104 49 1 14 117 4 106 6.27e-32 117
MS.gene49318.t1 AT2G23660 51.923 104 49 1 14 117 4 106 6.27e-32 117
MS.gene49318.t1 AT2G23660 51.923 104 49 1 14 117 4 106 6.27e-32 117
MS.gene49318.t1 AT3G26660 44.248 113 61 2 16 127 6 117 2.92e-30 108
MS.gene49318.t1 AT3G11090 47.788 113 57 2 5 117 2 112 3.13e-30 109
MS.gene49318.t1 AT3G26620 44.340 106 58 1 16 121 6 110 1.01e-29 107
MS.gene49318.t1 AT1G65620 61.176 85 32 1 11 95 5 88 1.77e-29 108
MS.gene49318.t1 AT1G65620 61.176 85 32 1 11 95 5 88 1.77e-29 108
MS.gene49318.t1 AT1G65620 61.176 85 32 1 11 95 5 88 1.77e-29 108
MS.gene49318.t1 AT1G65620 61.176 85 32 1 11 95 5 88 1.77e-29 108
MS.gene49318.t1 AT1G65620 61.176 85 32 1 11 95 5 88 1.77e-29 108
MS.gene49318.t1 AT2G40470 51.754 114 54 1 15 128 20 132 5.10e-29 107
MS.gene49318.t1 AT2G40470 51.754 114 54 1 15 128 45 157 7.64e-29 107
MS.gene49318.t1 AT3G50510 46.465 99 52 1 15 113 12 109 7.85e-23 91.3
MS.gene49318.t1 AT3G50510 46.465 99 52 1 15 113 12 109 7.85e-23 91.3
MS.gene49318.t1 AT1G72980 35.000 120 77 1 12 131 10 128 8.69e-17 75.9
MS.gene49318.t1 AT3G47870 30.070 143 82 2 16 141 37 178 1.10e-16 77.4
MS.gene49318.t1 AT5G35900 39.000 100 60 1 16 115 6 104 2.53e-16 74.3
MS.gene49318.t1 AT3G13850 37.255 102 62 2 16 117 37 136 2.87e-16 75.5
MS.gene49318.t1 AT1G06280 45.570 79 34 2 16 90 25 98 7.05e-16 73.2

Find 45 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTATTATAGATCCTGTTTA+TGG 0.144291 1.1:-52586375 MS.gene49318:CDS
TTCATATTTGTGGGACAAAA+TGG 0.226031 1.1:+52584675 None:intergenic
TAAAAGAATGTCATATGATT+TGG 0.238586 1.1:-52584559 MS.gene49318:CDS
AATGATGGGAAACTATAATT+AGG 0.332280 1.1:-52584511 None:intergenic
AGGGAGCTCTTAGGAGATAT+AGG 0.344167 1.1:+52584651 None:intergenic
TGAATAGCTGCAAATCTTGC+AGG 0.346463 1.1:+52586498 None:intergenic
CATGGACATCCTGAGGATCT+AGG 0.355593 1.1:+52586597 None:intergenic
AAAAGAATGTCATATGATTT+GGG 0.357241 1.1:-52584558 MS.gene49318:CDS
GCAGCTATTCATAAAGTGTT+TGG 0.364830 1.1:-52586486 MS.gene49318:CDS
CCTAGAGGAGACAGGGAACT+TGG 0.371537 1.1:-52584742 MS.gene49318:CDS
TGATTTATGGCTTCTACAAA+TGG 0.395681 1.1:-52586627 None:intergenic
GATATAGGGTTCATATTTGT+GGG 0.408424 1.1:+52584666 None:intergenic
ATTGAATCAAGGGAGCTCTT+AGG 0.410397 1.1:+52584642 None:intergenic
AACTTGGAGAATCAATGGTC+AGG 0.427708 1.1:-52584726 MS.gene49318:CDS
CCAAGTTCCCTGTCTCCTCT+AGG 0.427857 1.1:+52584742 None:intergenic
AGAAACATCTCTCGGAATGA+TGG 0.438279 1.1:-52584526 MS.gene49318:CDS
GCAATTGCGGCAACAGCATC+GGG 0.447204 1.1:+52586414 None:intergenic
AGATATAGGGTTCATATTTG+TGG 0.449622 1.1:+52584665 None:intergenic
GGGAGCTCTTAGGAGATATA+GGG 0.451691 1.1:+52584652 None:intergenic
TGTGATACACAACCATAAAC+AGG 0.455984 1.1:+52586363 None:intergenic
TGCTAAAATCAATTGAATCA+AGG 0.464352 1.1:+52584631 None:intergenic
GGTTCTAGCCCTAGATCCTC+AGG 0.488943 1.1:-52586606 MS.gene49318:CDS
GCTAAAATCAATTGAATCAA+GGG 0.496109 1.1:+52584632 None:intergenic
AGTCAAAGCCTAGAGGAGAC+AGG 0.500514 1.1:-52584750 MS.gene49318:CDS
AGCAATTGCGGCAACAGCAT+CGG 0.501002 1.1:+52586413 None:intergenic
CAGGGAACTTGGAGAATCAA+TGG 0.511861 1.1:-52584731 MS.gene49318:CDS
GAAACATCTCTCGGAATGAT+GGG 0.517494 1.1:-52584525 MS.gene49318:CDS
ATGGACATCCTGAGGATCTA+GGG 0.518326 1.1:+52586598 None:intergenic
AGGAATTTGCATGCCCCACA+TGG 0.523694 1.1:+52586579 None:intergenic
TGGTAAATTCAGTAGCATCT+TGG 0.557512 1.1:+52586449 None:intergenic
ACTTGGAGAATCAATGGTCA+GGG 0.580705 1.1:-52584725 MS.gene49318:CDS
GCATCGGGACGATCATGAGC+TGG 0.597406 1.1:+52586429 None:intergenic
GGGCATGCAAATTCCTCCGT+AGG 0.600566 1.1:-52586572 MS.gene49318:CDS
TTGAGCTTCATAAGCAATTG+CGG 0.600657 1.1:+52586401 None:intergenic
GATCCTCAGGATGTCCATGT+GGG 0.601232 1.1:-52586593 MS.gene49318:CDS
GTCAAAGCCTAGAGGAGACA+GGG 0.607444 1.1:-52584749 MS.gene49318:CDS
AGATCCTCAGGATGTCCATG+TGG 0.614428 1.1:-52586594 MS.gene49318:CDS
AGCACAACTAATGCAAGTGA+AGG 0.620211 1.1:-52584787 MS.gene49318:CDS
GAGCGGCAAGAAACATCTCT+CGG 0.627630 1.1:-52584534 MS.gene49318:CDS
ATTCAGAAGTACACTTCCTA+CGG 0.633068 1.1:+52586556 None:intergenic
AATAATTAGTCAAAGCCTAG+AGG 0.639523 1.1:-52584757 MS.gene49318:CDS
TGCCCCACATGGACATCCTG+AGG 0.640571 1.1:+52586590 None:intergenic
TGTCATATGATTTGGGTGAG+CGG 0.650337 1.1:-52584551 MS.gene49318:CDS
CAGAAGTACACTTCCTACGG+AGG 0.660431 1.1:+52586559 None:intergenic
ATCCTCAGGATGTCCATGTG+GGG 0.681917 1.1:-52586592 MS.gene49318:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTATATATAAGAAAAATTT+GGG + chr1.1:52586096-52586115 None:intergenic 10.0%
!! TTCTATATATAAGAAAAATT+TGG + chr1.1:52586097-52586116 None:intergenic 10.0%
!!! AAATATATCATTTTAATTTG+GGG + chr1.1:52585479-52585498 None:intergenic 10.0%
!!! AATCAATTAAAATTTGTTAA+TGG + chr1.1:52585347-52585366 None:intergenic 10.0%
!!! TAATATATAACTTCTTTTTA+AGG - chr1.1:52585152-52585171 MS.gene49318:intron 10.0%
!! GGATGAAATTTAATAAATAA+GGG + chr1.1:52585858-52585877 None:intergenic 15.0%
!! TGGATGAAATTTAATAAATA+AGG + chr1.1:52585859-52585878 None:intergenic 15.0%
!! TGTGTTAAAATGTTAATTAT+AGG + chr1.1:52584831-52584850 None:intergenic 15.0%
!!! AATAAATAAGGGTATAATAA+TGG + chr1.1:52585847-52585866 None:intergenic 15.0%
!!! ATTTTGAGAAAAAGAAAAAT+AGG + chr1.1:52585778-52585797 None:intergenic 15.0%
!! AAAAAAGCAAAACAAAATTG+TGG + chr1.1:52585100-52585119 None:intergenic 20.0%
!! AAAAGAATGTCATATGATTT+GGG - chr1.1:52586575-52586594 MS.gene49318:CDS 20.0%
!! AAAATTGTGGAAAAAATTTC+TGG + chr1.1:52585087-52585106 None:intergenic 20.0%
!! ACCTAATCAAAATACAAAAA+CGG + chr1.1:52586314-52586333 None:intergenic 20.0%
!! ATGTACCGTTATTTATTTAA+AGG + chr1.1:52586156-52586175 None:intergenic 20.0%
!! GTACTCCTTTAAATAAATAA+CGG - chr1.1:52586148-52586167 MS.gene49318:intron 20.0%
!! GTCATATTAATTGTTCATAT+GGG - chr1.1:52586252-52586271 MS.gene49318:intron 20.0%
!! TAAAAGAATGTCATATGATT+TGG - chr1.1:52586574-52586593 MS.gene49318:CDS 20.0%
!! TGTCATATTAATTGTTCATA+TGG - chr1.1:52586251-52586270 MS.gene49318:intron 20.0%
!!! ACAATTTTCTTCATACAAAA+AGG - chr1.1:52585551-52585570 MS.gene49318:intron 20.0%
!!! ACATATATGTGTTCAAATTT+TGG - chr1.1:52585201-52585220 MS.gene49318:intron 20.0%
!!! ATTAATTGTGTTGATTTTGA+TGG + chr1.1:52585942-52585961 None:intergenic 20.0%
!!! CAAGAATTACTTAGAATTTT+AGG + chr1.1:52585705-52585724 None:intergenic 20.0%
!!! CTGCAACTTTATTTTTATAT+AGG + chr1.1:52585025-52585044 None:intergenic 20.0%
! AAAAACAATAAATGCTGCAT+TGG + chr1.1:52585819-52585838 None:intergenic 25.0%
! ATAAATAACGGTACATTGAT+TGG - chr1.1:52586160-52586179 MS.gene49318:intron 25.0%
! CCACTAACTACAATTAATAA+GGG - chr1.1:52585890-52585909 MS.gene49318:intron 25.0%
! CCTAATCAAAATACAAAAAC+GGG + chr1.1:52586313-52586332 None:intergenic 25.0%
! CTAATCAAAATACAAAAACG+GGG + chr1.1:52586312-52586331 None:intergenic 25.0%
! GCTAAAATCAATTGAATCAA+GGG + chr1.1:52586504-52586523 None:intergenic 25.0%
! TCAACAAAAGTTAATGTATC+TGG - chr1.1:52586034-52586053 MS.gene49318:intron 25.0%
! TCCACTAACTACAATTAATA+AGG - chr1.1:52585889-52585908 MS.gene49318:intron 25.0%
! TGCTAAAATCAATTGAATCA+AGG + chr1.1:52586505-52586524 None:intergenic 25.0%
! TTCTTATATATAGAACCAGA+AGG - chr1.1:52586102-52586121 MS.gene49318:intron 25.0%
!! TTCTTTTATTAGAGTAAGCT+TGG + chr1.1:52586562-52586581 None:intergenic 25.0%
!!! TATTTTTGCTTTACAACAAC+AGG - chr1.1:52584793-52584812 MS.gene49318:CDS 25.0%
!!! TTTCATTCAGTTGTTTTCAA+GGG - chr1.1:52585061-52585080 MS.gene49318:intron 25.0%
AAGAGACAATTATTGTGAGA+CGG + chr1.1:52585753-52585772 None:intergenic 30.0%
AATAATTAGTCAAAGCCTAG+AGG - chr1.1:52586376-52586395 MS.gene49318:CDS 30.0%
AGATATAGGGTTCATATTTG+TGG + chr1.1:52586471-52586490 None:intergenic 30.0%
AGGTTTATGCACGATTATTA+AGG + chr1.1:52585970-52585989 None:intergenic 30.0%
CCCTTATTAATTGTAGTTAG+TGG + chr1.1:52585893-52585912 None:intergenic 30.0%
CCTTGTTCACAACAAAAATA+AGG + chr1.1:52584614-52584633 None:intergenic 30.0%
CGTATTATAGATCCTGTTTA+TGG - chr1.1:52584758-52584777 MS.gene49318:CDS 30.0%
CTGAAATGTCTCTTGTAAAT+AGG + chr1.1:52586014-52586033 None:intergenic 30.0%
GATATAGGGTTCATATTTGT+GGG + chr1.1:52586470-52586489 None:intergenic 30.0%
TACCTATGTATACAACAACA+GGG + chr1.1:52585279-52585298 None:intergenic 30.0%
TCTCTAAATAATACTCCTTC+TGG + chr1.1:52586120-52586139 None:intergenic 30.0%
TTCATATTTGTGGGACAAAA+TGG + chr1.1:52586461-52586480 None:intergenic 30.0%
TTGTTGTATACATAGGTAGT+AGG - chr1.1:52585281-52585300 MS.gene49318:intron 30.0%
!! CTTTCATTCAGTTGTTTTCA+AGG - chr1.1:52585060-52585079 MS.gene49318:intron 30.0%
!! GAAGGAGTATTATTTAGAGA+CGG - chr1.1:52586120-52586139 MS.gene49318:intron 30.0%
!! TCAACAAAAGTTGATGTATC+TGG + chr1.1:52586073-52586092 None:intergenic 30.0%
!! TGATTGGTGGATTTAAATTG+CGG - chr1.1:52586176-52586195 MS.gene49318:intron 30.0%
!!! CCCGTTTTTGTATTTTGATT+AGG - chr1.1:52586310-52586329 MS.gene49318:intron 30.0%
!!! CCTTATTTTTGTTGTGAACA+AGG - chr1.1:52584611-52584630 MS.gene49318:CDS 30.0%
ATCAACGAGTAAGAGTTGTT+TGG - chr1.1:52585419-52585438 MS.gene49318:intron 35.0%
ATGTCTCTTGTAAATAGGAC+CGG + chr1.1:52586009-52586028 None:intergenic 35.0%
CTACCTATGTATACAACAAC+AGG + chr1.1:52585280-52585299 None:intergenic 35.0%
GAGTATTATTTAGAGACGGA+GGG - chr1.1:52586124-52586143 MS.gene49318:intron 35.0%
GTATACATAGGTAGTAGGTT+TGG - chr1.1:52585286-52585305 MS.gene49318:intron 35.0%
TGGTAAATTCAGTAGCATCT+TGG + chr1.1:52584687-52584706 None:intergenic 35.0%
TGTGATACACAACCATAAAC+AGG + chr1.1:52584773-52584792 None:intergenic 35.0%
TTGAGCTTCATAAGCAATTG+CGG + chr1.1:52584735-52584754 None:intergenic 35.0%
! AATAACGGTACATTGATTGG+TGG - chr1.1:52586163-52586182 MS.gene49318:intron 35.0%
! ATTCAGAAGTACACTTCCTA+CGG + chr1.1:52584580-52584599 None:intergenic 35.0%
! GCAGCTATTCATAAAGTGTT+TGG - chr1.1:52584647-52584666 MS.gene49318:CDS 35.0%
!!! TGGGTTTTATGTTTGACTTG+TGG - chr1.1:52586271-52586290 MS.gene49318:intron 35.0%
AACTTGGAGAATCAATGGTC+AGG - chr1.1:52586407-52586426 MS.gene49318:CDS 40.0%
ACTTGGAGAATCAATGGTCA+GGG - chr1.1:52586408-52586427 MS.gene49318:CDS 40.0%
AGAAACATCTCTCGGAATGA+TGG - chr1.1:52586607-52586626 MS.gene49318:CDS 40.0%
AGACAATTATTGTGAGACGG+AGG + chr1.1:52585750-52585769 None:intergenic 40.0%
AGCACAACTAATGCAAGTGA+AGG - chr1.1:52586346-52586365 MS.gene49318:CDS 40.0%
AGTTAGTGGAGTAGTCAAGT+TGG + chr1.1:52585879-52585898 None:intergenic 40.0%
ATCGACGAGTAAGAGTTGTT+TGG - chr1.1:52585447-52585466 MS.gene49318:intron 40.0%
ATTGAATCAAGGGAGCTCTT+AGG + chr1.1:52586494-52586513 None:intergenic 40.0%
CTCCCTGTTGTTGTATACAT+AGG - chr1.1:52585274-52585293 MS.gene49318:intron 40.0%
GAAACATCTCTCGGAATGAT+GGG - chr1.1:52586608-52586627 MS.gene49318:CDS 40.0%
GACAATTATTGTGAGACGGA+GGG + chr1.1:52585749-52585768 None:intergenic 40.0%
GCAGTGCAAACGTACTATAA+AGG + chr1.1:52586210-52586229 None:intergenic 40.0%
TGAATAGCTGCAAATCTTGC+AGG + chr1.1:52584638-52584657 None:intergenic 40.0%
! GGAGTATTATTTAGAGACGG+AGG - chr1.1:52586123-52586142 MS.gene49318:intron 40.0%
! TGTCATATGATTTGGGTGAG+CGG - chr1.1:52586582-52586601 MS.gene49318:CDS 40.0%
AGGGAGCTCTTAGGAGATAT+AGG + chr1.1:52586485-52586504 None:intergenic 45.0%
ATGGACATCCTGAGGATCTA+GGG + chr1.1:52584538-52584557 None:intergenic 45.0%
CAGGGAACTTGGAGAATCAA+TGG - chr1.1:52586402-52586421 MS.gene49318:CDS 45.0%
CCTTAAACGACTACTTCCTC+CGG - chr1.1:52585987-52586006 MS.gene49318:intron 45.0%
GGGAGCTCTTAGGAGATATA+GGG + chr1.1:52586484-52586503 None:intergenic 45.0%
TCTCTTGTAAATAGGACCGG+AGG + chr1.1:52586006-52586025 None:intergenic 45.0%
!!! AAAAATATATCATTTTAATT+TGG + chr1.1:52585481-52585500 None:intergenic 5.0%
!!! AAAATATATCATTTTAATTT+GGG + chr1.1:52585480-52585499 None:intergenic 5.0%
AGATCCTCAGGATGTCCATG+TGG - chr1.1:52584539-52584558 MS.gene49318:CDS 50.0%
AGCAATTGCGGCAACAGCAT+CGG + chr1.1:52584723-52584742 None:intergenic 50.0%
AGGAATTTGCATGCCCCACA+TGG + chr1.1:52584557-52584576 None:intergenic 50.0%
AGTCAAAGCCTAGAGGAGAC+AGG - chr1.1:52586383-52586402 MS.gene49318:CDS 50.0%
ATCCTCAGGATGTCCATGTG+GGG - chr1.1:52584541-52584560 MS.gene49318:CDS 50.0%
CATGGACATCCTGAGGATCT+AGG + chr1.1:52584539-52584558 None:intergenic 50.0%
CCGGAGGAAGTAGTCGTTTA+AGG + chr1.1:52585990-52586009 None:intergenic 50.0%
GAGCGGCAAGAAACATCTCT+CGG - chr1.1:52586599-52586618 MS.gene49318:CDS 50.0%
GATCCTCAGGATGTCCATGT+GGG - chr1.1:52584540-52584559 MS.gene49318:CDS 50.0%
GTCAAAGCCTAGAGGAGACA+GGG - chr1.1:52586384-52586403 MS.gene49318:CDS 50.0%
! CAGAAGTACACTTCCTACGG+AGG + chr1.1:52584577-52584596 None:intergenic 50.0%
CCAAGTTCCCTGTCTCCTCT+AGG + chr1.1:52586394-52586413 None:intergenic 55.0%
CCTAGAGGAGACAGGGAACT+TGG - chr1.1:52586391-52586410 MS.gene49318:CDS 55.0%
GCAATTGCGGCAACAGCATC+GGG + chr1.1:52584722-52584741 None:intergenic 55.0%
GGGCATGCAAATTCCTCCGT+AGG - chr1.1:52584561-52584580 MS.gene49318:CDS 55.0%
! GGTTCTAGCCCTAGATCCTC+AGG - chr1.1:52584527-52584546 MS.gene49318:CDS 55.0%
GCATCGGGACGATCATGAGC+TGG + chr1.1:52584707-52584726 None:intergenic 60.0%
TGCCCCACATGGACATCCTG+AGG + chr1.1:52584546-52584565 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 52584512 52586643 52584512 ID=MS.gene49318
chr1.1 mRNA 52584512 52586643 52584512 ID=MS.gene49318.t1;Parent=MS.gene49318
chr1.1 exon 52586341 52586643 52586341 ID=MS.gene49318.t1.exon1;Parent=MS.gene49318.t1
chr1.1 CDS 52586341 52586643 52586341 ID=cds.MS.gene49318.t1;Parent=MS.gene49318.t1
chr1.1 exon 52584512 52584823 52584512 ID=MS.gene49318.t1.exon2;Parent=MS.gene49318.t1
chr1.1 CDS 52584512 52584823 52584512 ID=cds.MS.gene49318.t1;Parent=MS.gene49318.t1
Gene Sequence

>MS.gene49318

ATGGCTTCTACAAATGGTTCTAGCCCTAGATCCTCAGGATGTCCATGTGGGGCATGCAAATTCCTCCGTAGGAAGTGTACTTCTGAATGCATATTTGCACCTTATTTTTGTTGTGAACAAGGACCTGCAAGATTTGCAGCTATTCATAAAGTGTTTGGTGCTAGCAATGTTTCCAAGATGCTACTGAATTTACCAGCTCATGATCGTCCCGATGCTGTTGCCGCAATTGCTTATGAAGCTCAAGCTCGTATTATAGATCCTGTTTATGGTTGTGTATCACATATTTTTGCTTTACAACAACAGGTACGCTTTCAGACCTATAATTAACATTTTAACACAATAGTCACATAGAACTAAATTAATCTTTATTTTACGATCTTCTATTAATAGAGTTAAATATATTCTTTTGAAGTTACAATCTCAAATTCTTATACTTGAGCTGACATACGATTAATATAGAATTTTATTAATGAACTTTGAAAAAATAACAAGCAAATTTTTCAAAAACTTCCTATATAAAAATAAAGTTGCAGTTTGTAAATTATATTCTTTCATTCAGTTGTTTTCAAGGGCCAGAAATTTTTTCCACAATTTTGTTTTGCTTTTTTAGTATATATGATGTGAATGAATGTTATATAATTAATATATAACTTCTTTTTAAGGAAATTAATAGTATTTAATTTATACATACATATATGTGTTCAAATTTTGGAATCGATTGAGTCAGATTGATTTTTCATCATACTCATGTCTTGTCTTTTTCTCCCTGTTGTTGTATACATAGGTAGTAGGTTTGGCTGCTATATATTAAACATCAAGATCAGCTAGCAGTCCATTAACAAATTTTAATTGATTATAAATTTACTTATGTCAATTATCGACTAGTAAGACTTTTCTTTTTATCAAGATCAACGAGTAAGAGTTGTTTGGACAAAATCGACGAGTAAGAGTTGTTTGGACAAAACCCCAAATTAAAATGATATATTTTTTGCAAGTGTTTGAGTTGTTGTAGTTCGAAAGTAGATCAAAGTGAAAACTCACAATTTTCTTCATACAAAAAGGTATATTTTCTATATAGATACAATTAAAGAAATCTAAATTTTCTAATACAGTCATAATTAAAACTCACTTCTTTTTTTCTTCTTTTACAATTTTCTTTGATTAAAGCAATTCTAGACTTTTTAGTTTTACCTAAAATTCTAAGTAATTCTTGATTTATCTAATTTCAATTACTCCCTCCGTCTCACAATAATTGTCTCTTTACCTATTTTTCTTTTTCTCAAAATAATTGTCACTTTAGAATACCAATGCAGCATTTATTGTTTTTTTTTTCCATTATTATACCCTTATTTATTAAATTTCATCCAACTTGACTACTCCACTAACTACAATTAATAAGGGTGTTTTAGTAAATGATATTAATTTTACCATCAAAATCAACACAATTAATCATTTCCTTAATAATCGTGCATAAACCTTAAACGACTACTTCCTCCGGTCCTATTTACAAGAGACATTTCAGTCAACAAAAGTTAATGTATCTGGTCTAATTTTTTAACCAGATACATCAACTTTTGTTGACCCAAATTTTTCTTATATATAGAACCAGAAGGAGTATTATTTAGAGACGGAGGGAGTACTCCTTTAAATAAATAACGGTACATTGATTGGTGGATTTAAATTGCGGATGCGTAACCTTTATAGTACGTTTGCACTGCGAACGTTGAATATATACTTTTTGTCATATTAATTGTTCATATGGGTTTTATGTTTGACTTGTGGTTTTTGAAATATGCACCCCGTTTTTGTATTTTGATTAGGTAGCAAGCTTGCAAGCACAACTAATGCAAGTGAAGGCACAACAAATAATTAGTCAAAGCCTAGAGGAGACAGGGAACTTGGAGAATCAATGGTCAGGGAATGTTGTTAAACAACAATTAATCAATCCATTTTGTCCCACAAATATGAACCCTATATCTCCTAAGAGCTCCCTTGATTCAATTGATTTTAGCAGCTGCAGCATCAATGATGTAATGAGCATACAATGCCAAGCTTACTCTAATAAAAGAATGTCATATGATTTGGGTGAGCGGCAAGAAACATCTCTCGGAATGATGGGAAACTATAATTAG
Protein sequence

>MS.gene49318.t1

MASTNGSSPRSSGCPCGACKFLRRKCTSECIFAPYFCCEQGPARFAAIHKVFGASNVSKMLLNLPAHDRPDAVAAIAYEAQARIIDPVYGCVSHIFALQQQVASLQAQLMQVKAQQIISQSLEETGNLENQWSGNVVKQQLINPFCPTNMNPISPKSSLDSIDFSSCSINDVMSIQCQAYSNKRMSYDLGERQETSLGMMGNYN