AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49443

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49443.t1 MTR_1g094960 89.537 497 16 4 1 463 1 495 0.0 910
MS.gene49443.t1 MTR_1g094960 95.833 48 2 0 449 496 572 619 1.95e-20 95.1
MS.gene49443.t1 MTR_2g094570 61.411 482 147 6 10 458 58 533 0.0 565
MS.gene49443.t1 MTR_2g094570 61.411 482 147 6 10 458 2 477 0.0 565
MS.gene49443.t1 MTR_2g094570 61.411 482 147 6 10 458 2 477 0.0 564
MS.gene49443.t1 MTR_4g058930 65.570 456 117 11 8 427 1 452 0.0 552
MS.gene49443.t1 MTR_4g058930 65.570 456 117 11 8 427 1 452 0.0 551
MS.gene49443.t1 MTR_1g064430 54.820 529 174 13 13 484 34 554 0.0 528
MS.gene49443.t1 MTR_1g064430 60.440 455 134 8 13 427 21 469 0.0 528
MS.gene49443.t1 MTR_7g101280 55.556 459 159 11 10 427 22 476 8.64e-164 478
MS.gene49443.t1 MTR_0041s0110 55.556 459 159 11 10 427 22 476 8.64e-164 478
MS.gene49443.t1 MTR_7g101275 47.126 435 175 8 28 461 1 381 3.49e-125 371
MS.gene49443.t1 MTR_0041s0120 46.667 435 189 10 28 461 1 393 1.71e-118 354
MS.gene49443.t1 MTR_3g073420 44.222 450 195 9 16 426 16 448 6.75e-114 348
MS.gene49443.t1 MTR_5g061220 42.963 405 190 11 16 408 16 391 2.37e-96 301
MS.gene49443.t1 MTR_2g024430 40.943 403 160 11 16 402 17 357 2.13e-86 275
MS.gene49443.t1 MTR_5g040880 36.887 469 185 12 11 426 3 413 7.28e-85 271
MS.gene49443.t1 MTR_5g076270 39.386 391 191 7 9 391 14 366 6.70e-83 274
MS.gene49443.t1 MTR_4g124900 40.000 380 182 8 13 385 17 357 4.00e-82 276
MS.gene49443.t1 MTR_4g124900 40.000 380 182 8 13 385 17 357 4.55e-82 276
MS.gene49443.t1 MTR_2g014770 39.257 377 181 7 15 383 51 387 5.40e-81 264
MS.gene49443.t1 MTR_2g014770 39.257 377 181 7 15 383 51 387 2.72e-80 263
MS.gene49443.t1 MTR_3g064050 39.018 387 190 7 13 391 20 368 5.92e-80 266
MS.gene49443.t1 MTR_2g018690 37.965 403 203 8 9 403 13 376 7.74e-80 267
MS.gene49443.t1 MTR_5g082140 35.714 462 211 11 8 435 1 410 4.60e-79 254
MS.gene49443.t1 MTR_8g079492 37.562 402 205 8 9 403 13 375 1.27e-78 264
MS.gene49443.t1 MTR_8g079492 37.562 402 205 8 9 403 13 375 1.85e-78 263
MS.gene49443.t1 MTR_1g058210 36.725 403 202 8 14 410 7 362 8.53e-78 251
MS.gene49443.t1 MTR_4g088210 36.152 473 218 14 12 459 33 446 8.63e-78 257
MS.gene49443.t1 MTR_4g088210 36.152 473 218 14 12 459 33 446 1.09e-77 257
MS.gene49443.t1 MTR_1g024025 38.750 400 192 11 14 403 38 394 1.17e-74 256
MS.gene49443.t1 MTR_2g093740 38.579 394 182 8 19 393 56 408 1.47e-74 249
MS.gene49443.t1 MTR_2g043250 38.259 379 188 7 14 385 18 357 1.97e-74 254
MS.gene49443.t1 MTR_2g093740 38.579 394 182 8 19 393 56 408 4.98e-74 248
MS.gene49443.t1 MTR_8g100050 37.157 401 202 7 10 401 50 409 1.04e-73 247
MS.gene49443.t1 MTR_2g093740 38.579 394 182 8 19 393 56 408 1.27e-73 248
MS.gene49443.t1 MTR_8g100050 37.157 401 202 7 10 401 50 409 4.89e-73 247
MS.gene49443.t1 MTR_5g040740 39.142 373 155 10 16 373 9 324 3.38e-72 235
MS.gene49443.t1 MTR_2g006270 34.043 470 231 14 19 470 13 421 4.35e-72 236
MS.gene49443.t1 MTR_8g101360 38.760 387 189 8 9 386 21 368 2.62e-71 246
MS.gene49443.t1 MTR_2g093740 37.346 407 182 9 19 393 56 421 6.48e-71 239
MS.gene49443.t1 MTR_4g060460 38.557 402 190 9 19 403 54 415 1.28e-70 240
MS.gene49443.t1 MTR_4g060460 38.557 402 190 9 19 403 54 415 1.54e-70 239
MS.gene49443.t1 MTR_2g093740 37.346 407 182 9 19 393 56 421 2.97e-70 238
MS.gene49443.t1 MTR_2g093740 37.346 407 182 9 19 393 56 421 6.18e-70 239
MS.gene49443.t1 MTR_2g005240 36.493 422 209 9 11 420 15 389 1.29e-69 235
MS.gene49443.t1 MTR_4g021580 37.736 424 208 12 15 425 15 395 4.73e-69 232
MS.gene49443.t1 MTR_4g021580 37.736 424 208 12 15 425 15 395 9.45e-69 233
MS.gene49443.t1 MTR_7g062540 33.402 482 251 13 8 470 3 433 3.20e-63 218
MS.gene49443.t1 MTR_8g027440 37.055 421 211 11 16 426 26 402 6.26e-62 214
MS.gene49443.t1 MTR_8g079492 38.182 330 164 6 75 403 3 293 2.60e-61 215
MS.gene49443.t1 MTR_2g006380 31.857 474 229 16 19 470 20 421 4.93e-61 207
MS.gene49443.t1 MTR_5g061890 35.052 388 182 14 16 389 9 340 2.61e-57 195
MS.gene49443.t1 MTR_5g060780 34.465 383 182 13 16 384 9 336 1.19e-56 194
MS.gene49443.t1 MTR_2g093740 40.217 276 129 3 119 393 38 278 1.40e-55 196
MS.gene49443.t1 MTR_5g460920 34.465 383 182 13 16 384 9 336 2.09e-55 190
MS.gene49443.t1 MTR_2g093740 40.217 276 129 3 119 393 38 278 3.00e-55 196
MS.gene49443.t1 MTR_2g093740 38.408 289 129 4 119 393 38 291 7.18e-52 186
MS.gene49443.t1 MTR_2g093740 38.408 289 129 4 119 393 38 291 1.23e-51 186
MS.gene49443.t1 MTR_8g446900 32.615 325 176 7 59 383 7 288 1.52e-47 169
MS.gene49443.t1 MTR_5g062970 41.624 197 105 4 16 207 35 226 2.82e-47 164
MS.gene49443.t1 MTR_5g060630 31.829 421 208 15 16 400 9 386 3.19e-46 169
MS.gene49443.t1 MTR_5g460740 31.829 421 208 15 16 400 9 386 3.19e-46 169
MS.gene49443.t1 MTR_5g062960 37.500 160 88 4 277 427 1 157 1.06e-23 99.0
MS.gene49443.t1 MTR_5g060770 34.437 151 87 4 63 213 6 144 1.61e-17 84.3
MS.gene49443.t1 MTR_3g064050 38.462 104 62 2 289 391 29 131 1.15e-14 77.0
MS.gene49443.t1 MTR_5g032260 40.426 94 54 2 277 369 49 141 2.49e-12 68.9
MS.gene49443.t1 MTR_1g058350 32.099 162 88 5 114 272 71 213 2.15e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49443.t1 AT4G30080 65.412 425 133 5 1 418 1 418 0.0 561
MS.gene49443.t1 AT2G28350 67.554 413 100 8 13 405 4 402 0.0 541
MS.gene49443.t1 AT2G28350 67.554 413 100 8 13 405 4 402 0.0 541
MS.gene49443.t1 AT1G77850 44.898 392 197 7 16 401 16 394 3.20e-111 342
MS.gene49443.t1 AT1G77850 44.898 392 197 7 16 401 16 394 3.44e-111 342
MS.gene49443.t1 AT5G37020 39.018 387 190 7 13 391 16 364 2.79e-82 271
MS.gene49443.t1 AT5G37020 39.018 387 190 7 13 391 16 364 8.70e-82 270
MS.gene49443.t1 AT1G30330 39.109 404 198 9 9 403 14 378 1.85e-79 266
MS.gene49443.t1 AT1G30330 39.109 404 198 9 9 403 14 378 1.85e-79 266
MS.gene49443.t1 AT1G30330 38.596 399 198 8 13 403 17 376 2.83e-79 266
MS.gene49443.t1 AT1G19220 38.918 388 191 8 5 385 9 357 1.01e-78 266
MS.gene49443.t1 AT5G20730 38.308 402 190 11 13 403 18 372 4.75e-76 259
MS.gene49443.t1 AT5G20730 38.060 402 192 11 13 403 18 373 6.62e-76 259
MS.gene49443.t1 AT5G20730 38.060 402 192 11 13 403 18 373 7.28e-76 259
MS.gene49443.t1 AT1G19850 39.164 383 183 8 11 383 35 377 8.57e-76 256
MS.gene49443.t1 AT1G19850 39.164 383 183 8 11 383 46 388 9.31e-76 256
MS.gene49443.t1 AT5G60450 36.967 422 203 9 8 409 30 408 1.17e-75 253
MS.gene49443.t1 AT2G33860 36.712 444 224 12 15 449 52 447 6.33e-75 248
MS.gene49443.t1 AT5G60450 37.470 411 196 8 18 409 64 432 1.73e-74 250
MS.gene49443.t1 AT5G62000 34.632 462 250 8 13 466 55 472 4.27e-73 248
MS.gene49443.t1 AT5G62000 34.632 462 250 8 13 466 55 472 4.27e-73 248
MS.gene49443.t1 AT5G62000 34.632 462 250 8 13 466 55 472 4.27e-73 248
MS.gene49443.t1 AT5G62000 34.632 462 250 8 13 466 55 472 4.27e-73 248
MS.gene49443.t1 AT5G62000 34.894 470 238 10 13 466 55 472 4.50e-73 248
MS.gene49443.t1 AT1G59750 39.005 382 183 8 15 387 18 358 7.85e-69 233
MS.gene49443.t1 AT1G59750 39.005 382 183 8 15 387 18 358 8.40e-69 233
MS.gene49443.t1 AT1G59750 39.005 382 183 8 15 387 18 358 9.35e-69 233
MS.gene49443.t1 AT1G59750 39.005 382 183 8 15 387 18 358 9.35e-69 233
MS.gene49443.t1 AT2G46530 38.155 401 178 14 16 404 18 360 5.88e-67 226
MS.gene49443.t1 AT3G61830 36.855 407 197 10 7 403 13 369 6.38e-67 226
MS.gene49443.t1 AT2G46530 38.155 401 178 14 16 404 39 381 9.96e-67 226
MS.gene49443.t1 AT4G23980 38.021 384 186 11 16 390 9 349 2.59e-65 223
MS.gene49443.t1 AT4G23980 38.021 384 186 11 16 390 9 349 3.20e-65 223
MS.gene49443.t1 AT5G37020 37.785 307 152 5 86 391 1 269 2.93e-57 202
MS.gene49443.t1 AT1G35540 34.390 410 214 11 12 413 20 382 7.43e-56 196
MS.gene49443.t1 AT1G35520 35.897 390 193 12 12 393 20 360 1.47e-55 196
MS.gene49443.t1 AT1G34310 35.897 390 193 11 12 393 20 360 2.03e-55 195
MS.gene49443.t1 AT3G61830 37.752 347 163 9 60 403 22 318 3.28e-55 194
MS.gene49443.t1 AT1G35240 35.433 381 189 12 12 383 20 352 8.19e-55 193
MS.gene49443.t1 AT1G34390 35.385 390 193 12 12 393 20 358 6.67e-54 191
MS.gene49443.t1 AT2G33860 36.747 332 166 8 119 449 2 290 1.27e-53 187
MS.gene49443.t1 AT1G34170 33.417 398 206 12 19 406 25 373 2.14e-53 188
MS.gene49443.t1 AT1G34410 35.000 380 192 11 12 383 20 352 5.56e-53 189
MS.gene49443.t1 AT2G46530 40.520 269 116 8 118 383 36 263 1.11e-48 175
MS.gene49443.t1 AT1G34170 31.910 398 186 12 19 406 25 347 8.34e-46 167
MS.gene49443.t1 AT1G34170 31.407 398 188 11 19 406 25 347 3.93e-45 166
MS.gene49443.t1 AT1G43950 40.465 215 108 7 12 218 20 222 1.29e-38 140
MS.gene49443.t1 AT2G36080 34.234 111 69 2 113 219 32 142 9.50e-12 63.9
MS.gene49443.t1 AT2G36080 34.234 111 69 2 113 219 32 142 9.50e-12 63.9
MS.gene49443.t1 AT2G36080 34.234 111 69 2 113 219 32 142 1.87e-11 64.7
MS.gene49443.t1 AT2G36080 34.234 111 69 2 113 219 32 142 2.02e-11 64.7
MS.gene49443.t1 AT3G25730 32.824 131 76 2 119 247 183 303 3.21e-11 65.1
MS.gene49443.t1 AT2G46870 34.906 106 62 2 111 216 27 125 6.31e-11 63.9
MS.gene49443.t1 AT1G01030 36.842 95 59 1 114 208 51 144 9.80e-11 63.5

Find 98 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAGGATTAGTTGGTTTA+TGG 0.157206 1.1:+68356270 MS.gene49443:CDS
TTTGAAGAAGATTGTTTCTT+TGG 0.183552 1.1:+68355566 MS.gene49443:CDS
ACAAACCTTGGTTTCTTTCT+TGG 0.220376 1.1:-68357107 None:intergenic
AAATCAGATGGATTATTATT+TGG 0.221410 1.1:-68357308 None:intergenic
GAGATCTTTGTGTTGGTATA+AGG 0.249926 1.1:+68355906 MS.gene49443:CDS
ACGTAGACTTCATCAGTTTC+AGG 0.253211 1.1:-68355503 None:intergenic
GAGAGGTGGAACACGGGTTT+TGG 0.257788 1.1:-68355441 None:intergenic
TGATGCAAAAGAACTATTAT+TGG 0.261306 1.1:-68357252 None:intergenic
CTACAACAAGACATGTTTCT+TGG 0.267352 1.1:+68357350 MS.gene49443:CDS
TTCTCCTTGGCGCCTTCTTC+AGG 0.267828 1.1:+68356339 MS.gene49443:CDS
TTGTTTGGTGGTGGTGGTTC+TGG 0.287312 1.1:+68356007 MS.gene49443:CDS
TGGAAACAATAATGGCTTAG+AGG 0.299971 1.1:+68355586 MS.gene49443:CDS
GGAGCCAGGCATGCTCAATT+TGG 0.304628 1.1:+68357284 MS.gene49443:CDS
TTGAAACTGAGGATTCTTCT+AGG 0.307810 1.1:+68356254 MS.gene49443:CDS
TGTTTCTTTGGAAACAATAA+TGG 0.309227 1.1:+68355578 MS.gene49443:CDS
TCTAGGATTAGTTGGTTTAT+GGG 0.316818 1.1:+68356271 MS.gene49443:CDS
GCTGTAAACTGTGCTGTTAA+TGG 0.368748 1.1:+68356109 MS.gene49443:CDS
TGGAGGCTCTCCTTGGTATA+AGG 0.368842 1.1:+68357700 MS.gene49443:CDS
TTCTTTCTTGGAGGTGTGAA+TGG 0.380455 1.1:-68357095 None:intergenic
TTTCTAATTCTTCTAGTACT+TGG 0.397904 1.1:+68355969 MS.gene49443:CDS
ATGCAAATTCAATGGTGTTC+TGG 0.400255 1.1:+68356211 MS.gene49443:CDS
GGAGAATCAGGCCAACGAAT+TGG 0.402668 1.1:-68356322 None:intergenic
AGGATTCTTCTAGGATTAGT+TGG 0.407748 1.1:+68356263 MS.gene49443:CDS
TTCGTTGGCCTGATTCTCCT+TGG 0.409284 1.1:+68356326 MS.gene49443:CDS
AGAGCAAAGAAAGGTGGAAT+TGG 0.414735 1.1:+68355929 MS.gene49443:CDS
AGAAGGCGCCAAGGAGAATC+AGG 0.419546 1.1:-68356334 None:intergenic
AAATGGTGGTAATGATTTGA+TGG 0.424677 1.1:+68356057 MS.gene49443:CDS
CAACACAAGCCTGATCTTGT+TGG 0.424795 1.1:+68357728 MS.gene49443:CDS
AACCTTGGTTTCTTTCTTGG+AGG 0.432289 1.1:-68357104 None:intergenic
TTAGCAATAATTGTCTGAAC+AGG 0.436415 1.1:-68355737 None:intergenic
CCATCAGAAGAATTCTCAAC+TGG 0.442393 1.1:-68357680 None:intergenic
AATGTCTTTGCAAAAGAAGC+TGG 0.444449 1.1:-68355620 None:intergenic
GAAACCTTAACACAAAATTC+AGG 0.450898 1.1:-68356172 None:intergenic
ATAGTCTCAGCACAGTATCT+AGG 0.458408 1.1:-68355683 None:intergenic
TGGTCCAAATTGAGCATGCC+TGG 0.458791 1.1:-68357288 None:intergenic
CTTCTGATGGAGGCTCTCCT+TGG 0.459576 1.1:+68357693 MS.gene49443:CDS
CGTAGACTTCATCAGTTTCA+GGG 0.459745 1.1:-68355502 None:intergenic
TCACCTTTGTTTGGTGGTGG+TGG 0.463200 1.1:+68356001 MS.gene49443:CDS
AGTGCTTAGATTCACAACTA+TGG 0.464164 1.1:+68355315 MS.gene49443:CDS
AACCGTGTATCACCTTTGTT+TGG 0.470136 1.1:+68355992 MS.gene49443:CDS
AAATGTTACTGCATTGTGTA+TGG 0.470847 1.1:+68357757 MS.gene49443:CDS
TTGAAGGGAATTAAATCAGA+TGG 0.471043 1.1:-68357320 None:intergenic
GTTTGTAGTTATGGATTCCA+TGG 0.477031 1.1:+68355277 MS.gene49443:CDS
AAGTTTAGACATATCTATAG+AGG 0.484340 1.1:+68355782 MS.gene49443:CDS
AATGGTGAGAGATTGAAGTT+TGG 0.485606 1.1:-68357077 None:intergenic
AATCACAAGAAGCTTGTTGC+TGG 0.491007 1.1:+68355851 MS.gene49443:CDS
AATCTGCAATGCAAATTCAA+TGG 0.492272 1.1:+68356203 MS.gene49443:CDS
GAAGAATCCTCAGTTTCAAA+AGG 0.493790 1.1:-68356250 None:intergenic
CATCAAGCATACAGGGAGCC+AGG 0.498276 1.1:+68357270 MS.gene49443:CDS
TTGTTGTAGTAGCTTGTTGA+AGG 0.499531 1.1:-68357336 None:intergenic
AATTCAGGAGTACTAGCTCT+TGG 0.500520 1.1:-68356157 None:intergenic
CGTGTATCACCTTTGTTTGG+TGG 0.513472 1.1:+68355995 MS.gene49443:CDS
ATAATATTGTTACCTGAAGA+AGG 0.514309 1.1:-68356351 None:intergenic
CAACTATGGCACGCATGTGC+TGG 0.516615 1.1:+68355329 MS.gene49443:CDS
GGAAACAATAATGGCTTAGA+GGG 0.522828 1.1:+68355587 MS.gene49443:CDS
GGTGGTTCTGGTTTCTTGTG+TGG 0.526295 1.1:+68356019 MS.gene49443:CDS
AGGATATGCATGGACAGTGT+TGG 0.527527 1.1:+68355759 MS.gene49443:CDS
GCAAAGAAAGGTGGAATTGG+TGG 0.533246 1.1:+68355932 MS.gene49443:CDS
TAATGATTTGATGGGAAAAG+TGG 0.533977 1.1:+68356066 MS.gene49443:CDS
CCAGTTGAGAATTCTTCTGA+TGG 0.534523 1.1:+68357680 MS.gene49443:CDS
GTATCACCTTTGTTTGGTGG+TGG 0.534706 1.1:+68355998 MS.gene49443:CDS
GAAAATGGAGATCTTTGTGT+TGG 0.538803 1.1:+68355899 MS.gene49443:CDS
TGTTCAGACAATTATTGCTA+AGG 0.546499 1.1:+68355739 MS.gene49443:CDS
TTCATAGCAGAGATTCTACA+AGG 0.548674 1.1:-68355467 None:intergenic
TGTTGTTGATCCTTATACCA+AGG 0.550241 1.1:-68357710 None:intergenic
GTTGTCAAAAGATGTCTCCT+TGG 0.557297 1.1:-68355809 None:intergenic
AAGAAAGGTGGAATTGGTGG+TGG 0.559157 1.1:+68355935 MS.gene49443:CDS
GCAATAATTGTCTGAACAGG+TGG 0.564242 1.1:-68355734 None:intergenic
GAGACTAATTCAACTAACCA+AGG 0.569877 1.1:-68357047 None:intergenic
GCACGCATGTGCTGGTGCCA+TGG 0.574468 1.1:+68355337 MS.gene49443:CDS
ATAAGGAGAGCAAAGAAAGG+TGG 0.575173 1.1:+68355923 MS.gene49443:CDS
TGTTGTAGTAGCTTGTTGAA+GGG 0.575894 1.1:-68357335 None:intergenic
GGTATAAGGAGAGCAAAGAA+AGG 0.576664 1.1:+68355920 MS.gene49443:CDS
ATTATTGCTAAGGATATGCA+TGG 0.587806 1.1:+68355749 MS.gene49443:CDS
ATGTTCAAGATCCAATTCGT+TGG 0.592882 1.1:+68356311 MS.gene49443:CDS
GTTGAGAATTCTTCTGATGG+AGG 0.594043 1.1:+68357683 MS.gene49443:CDS
GAGATTCTACAAGGGATGAG+AGG 0.595750 1.1:-68355458 None:intergenic
TCTGCTGAATAATCCAACCT+AGG 0.603231 1.1:-68355710 None:intergenic
GCATGTTCTGCATGACCTTG+TGG 0.603875 1.1:-68355398 None:intergenic
AGACTAATTCAACTAACCAA+GGG 0.605653 1.1:-68357046 None:intergenic
ATGTGAAATGTGTAAACCCT+TGG 0.607377 1.1:+68357030 MS.gene49443:CDS
AATCTCTGCTATGAAATACA+TGG 0.608031 1.1:+68355475 MS.gene49443:CDS
AATGGTGGTAATGATTTGAT+GGG 0.610306 1.1:+68356058 MS.gene49443:CDS
AAGGGATGAGAGGTGGAACA+CGG 0.623921 1.1:-68355448 None:intergenic
GAACCACCACCACCAAACAA+AGG 0.625620 1.1:-68356004 None:intergenic
CACCAAACAAAGGTGATACA+CGG 0.628457 1.1:-68355994 None:intergenic
ACACAATCTGATGCAAACAA+TGG 0.631167 1.1:+68355644 MS.gene49443:CDS
ATTCTACAAGGGATGAGAGG+TGG 0.633006 1.1:-68355455 None:intergenic
AATGATTTGATGGGAAAAGT+GGG 0.635170 1.1:+68356067 MS.gene49443:CDS
CACCTCCAAGAAAGAAACCA+AGG 0.638580 1.1:+68357102 MS.gene49443:CDS
GTTACCTGAAGAAGGCGCCA+AGG 0.651106 1.1:-68356343 None:intergenic
GAGTGGTGGCATTTGAACCA+TGG 0.651177 1.1:-68355354 None:intergenic
AGGGATGAGAGGTGGAACAC+GGG 0.668889 1.1:-68355447 None:intergenic
AAAGTCTTCTACTTTCCACA+AGG 0.669890 1.1:+68355383 MS.gene49443:CDS
TCATAGCAGAGATTCTACAA+GGG 0.672161 1.1:-68355466 None:intergenic
TCTGATGCAAACAATGGTGG+TGG 0.672693 1.1:+68355650 MS.gene49443:CDS
CAATCTGATGCAAACAATGG+TGG 0.683286 1.1:+68355647 MS.gene49443:CDS
ATATCTATAGAGGAACACCA+AGG 0.796890 1.1:+68355792 MS.gene49443:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTAATTCTTGTTTAAC+AGG - chr1.1:68356700-68356719 None:intergenic 15.0%
!!! TTTTTCCAAATAACAAAATA+TGG - chr1.1:68357565-68357584 None:intergenic 15.0%
!! ATAATTGAGAAGATTAATGT+TGG - chr1.1:68357198-68357217 None:intergenic 20.0%
!! CAAATAACAAAATATGGTTA+TGG - chr1.1:68357559-68357578 None:intergenic 20.0%
!! TAAGTTTATACCTACTTAAT+TGG + chr1.1:68356902-68356921 MS.gene49443:intron 20.0%
!!! AAATCAGATGGATTATTATT+TGG - chr1.1:68357311-68357330 None:intergenic 20.0%
!!! CATAACCATATTTTGTTATT+TGG + chr1.1:68357557-68357576 MS.gene49443:intron 20.0%
! AAAATATGGTTATGGTTATG+GGG - chr1.1:68357551-68357570 None:intergenic 25.0%
! AACTTAATAACTTTCTCTCA+AGG - chr1.1:68356888-68356907 None:intergenic 25.0%
! AAGTTTAGACATATCTATAG+AGG + chr1.1:68355782-68355801 MS.gene49443:CDS 25.0%
! ACAAAATATGGTTATGGTTA+TGG - chr1.1:68357553-68357572 None:intergenic 25.0%
! AGTTCAAATATTTCAAAGAG+TGG + chr1.1:68357605-68357624 MS.gene49443:intron 25.0%
! ATAATATTGTTACCTGAAGA+AGG - chr1.1:68356354-68356373 None:intergenic 25.0%
! CAAAATATGGTTATGGTTAT+GGG - chr1.1:68357552-68357571 None:intergenic 25.0%
! TGATGCAAAAGAACTATTAT+TGG - chr1.1:68357255-68357274 None:intergenic 25.0%
! TGATTTATGAGGTGAAAATA+TGG - chr1.1:68357140-68357159 None:intergenic 25.0%
! TTTCTAATTCTTCTAGTACT+TGG + chr1.1:68355969-68355988 MS.gene49443:CDS 25.0%
!! AAATTCTGGTCAAAGTTTTA+AGG + chr1.1:68357502-68357521 MS.gene49443:intron 25.0%
!! ATTGTTATTGATACTCTTGT+AGG - chr1.1:68357435-68357454 None:intergenic 25.0%
!! TGTTTCTTTGGAAACAATAA+TGG + chr1.1:68355578-68355597 MS.gene49443:CDS 25.0%
!! TTGTTATTGATACTCTTGTA+GGG - chr1.1:68357434-68357453 None:intergenic 25.0%
!! TTTGAAGAAGATTGTTTCTT+TGG + chr1.1:68355566-68355585 MS.gene49443:CDS 25.0%
!!! TTCACATTTTGAAGTAAATC+TGG - chr1.1:68357017-68357036 None:intergenic 25.0%
AAATGTTACTGCATTGTGTA+TGG + chr1.1:68357757-68357776 MS.gene49443:CDS 30.0%
AATCTCTGCTATGAAATACA+TGG + chr1.1:68355475-68355494 MS.gene49443:CDS 30.0%
AATCTGCAATGCAAATTCAA+TGG + chr1.1:68356203-68356222 MS.gene49443:CDS 30.0%
AATGACAACAGAAAAAATGG+TGG + chr1.1:68356043-68356062 MS.gene49443:CDS 30.0%
AATGATTTGATGGGAAAAGT+GGG + chr1.1:68356067-68356086 MS.gene49443:CDS 30.0%
AATTCAGTGCATCAATAAGA+TGG + chr1.1:68356533-68356552 MS.gene49443:intron 30.0%
AGACTAATTCAACTAACCAA+GGG - chr1.1:68357049-68357068 None:intergenic 30.0%
AGATTAATGTTGGAGATTGA+TGG - chr1.1:68357188-68357207 None:intergenic 30.0%
ATCATCAACAACCTATAACT+AGG + chr1.1:68357396-68357415 MS.gene49443:intron 30.0%
ATTATTGCTAAGGATATGCA+TGG + chr1.1:68355749-68355768 MS.gene49443:CDS 30.0%
GAAACCTTAACACAAAATTC+AGG - chr1.1:68356175-68356194 None:intergenic 30.0%
GGAAATGACAACAGAAAAAA+TGG + chr1.1:68356040-68356059 MS.gene49443:CDS 30.0%
TAATGATTTGATGGGAAAAG+TGG + chr1.1:68356066-68356085 MS.gene49443:CDS 30.0%
TGTTCAGACAATTATTGCTA+AGG + chr1.1:68355739-68355758 MS.gene49443:CDS 30.0%
TTACTTCAAGCCAATTAAGT+AGG - chr1.1:68356915-68356934 None:intergenic 30.0%
TTAGCAATAATTGTCTGAAC+AGG - chr1.1:68355740-68355759 None:intergenic 30.0%
TTGAAGGGAATTAAATCAGA+TGG - chr1.1:68357323-68357342 None:intergenic 30.0%
! AAATGGTGGTAATGATTTGA+TGG + chr1.1:68356057-68356076 MS.gene49443:CDS 30.0%
! AATGGTGGTAATGATTTGAT+GGG + chr1.1:68356058-68356077 MS.gene49443:CDS 30.0%
! CTAACTCGTTTTCTCTTAAA+GGG - chr1.1:68355544-68355563 None:intergenic 30.0%
! TAACTCGTTTTCTCTTAAAG+GGG - chr1.1:68355543-68355562 None:intergenic 30.0%
! TACTCCTGAATTTTGTGTTA+AGG + chr1.1:68356168-68356187 MS.gene49443:CDS 30.0%
! TCTAACTCGTTTTCTCTTAA+AGG - chr1.1:68355545-68355564 None:intergenic 30.0%
! TGTTAATGGAAGATCTTTTG+AGG + chr1.1:68356123-68356142 MS.gene49443:CDS 30.0%
! TTTATGATGTCATGTTTTCC+AGG + chr1.1:68356977-68356996 MS.gene49443:intron 30.0%
!! TCTAGGATTAGTTGGTTTAT+GGG + chr1.1:68356271-68356290 MS.gene49443:CDS 30.0%
!! TTCTAGGATTAGTTGGTTTA+TGG + chr1.1:68356270-68356289 MS.gene49443:CDS 30.0%
AAAGTCTTCTACTTTCCACA+AGG + chr1.1:68355383-68355402 MS.gene49443:CDS 35.0%
AATGGTGAGAGATTGAAGTT+TGG - chr1.1:68357080-68357099 None:intergenic 35.0%
AATGTCTTTGCAAAAGAAGC+TGG - chr1.1:68355623-68355642 None:intergenic 35.0%
ACAAACCTTGGTTTCTTTCT+TGG - chr1.1:68357110-68357129 None:intergenic 35.0%
ACACAATCTGATGCAAACAA+TGG + chr1.1:68355644-68355663 MS.gene49443:CDS 35.0%
AGTGCTTAGATTCACAACTA+TGG + chr1.1:68355315-68355334 MS.gene49443:CDS 35.0%
ATATCTATAGAGGAACACCA+AGG + chr1.1:68355792-68355811 MS.gene49443:CDS 35.0%
ATGCAAATTCAATGGTGTTC+TGG + chr1.1:68356211-68356230 MS.gene49443:CDS 35.0%
ATGTGAAATGTGTAAACCCT+TGG + chr1.1:68357030-68357049 MS.gene49443:CDS 35.0%
ATGTTCAAGATCCAATTCGT+TGG + chr1.1:68356311-68356330 MS.gene49443:CDS 35.0%
ATTCTAGCAAGTAGCAACTA+TGG - chr1.1:68356856-68356875 None:intergenic 35.0%
CTACAACAAGACATGTTTCT+TGG + chr1.1:68357350-68357369 MS.gene49443:CDS 35.0%
CTATGGTAGATAGTACTAGT+AGG - chr1.1:68356839-68356858 None:intergenic 35.0%
GAAAATGGAGATCTTTGTGT+TGG + chr1.1:68355899-68355918 MS.gene49443:CDS 35.0%
GAAGAATCCTCAGTTTCAAA+AGG - chr1.1:68356253-68356272 None:intergenic 35.0%
GAGACTAATTCAACTAACCA+AGG - chr1.1:68357050-68357069 None:intergenic 35.0%
GGAAACAATAATGGCTTAGA+GGG + chr1.1:68355587-68355606 MS.gene49443:CDS 35.0%
GTTTGTAGTTATGGATTCCA+TGG + chr1.1:68355277-68355296 MS.gene49443:CDS 35.0%
TATGGATCTTGAACAAACCT+TGG - chr1.1:68357122-68357141 None:intergenic 35.0%
TCATAGCAGAGATTCTACAA+GGG - chr1.1:68355469-68355488 None:intergenic 35.0%
TGGAAACAATAATGGCTTAG+AGG + chr1.1:68355586-68355605 MS.gene49443:CDS 35.0%
TGTTGTTGATCCTTATACCA+AGG - chr1.1:68357713-68357732 None:intergenic 35.0%
TTCATAGCAGAGATTCTACA+AGG - chr1.1:68355470-68355489 None:intergenic 35.0%
TTGAAACTGAGGATTCTTCT+AGG + chr1.1:68356254-68356273 MS.gene49443:CDS 35.0%
! AGTTGCTACTTGCTAGAATT+TGG + chr1.1:68356857-68356876 MS.gene49443:intron 35.0%
! GAGATCTTTGTGTTGGTATA+AGG + chr1.1:68355906-68355925 MS.gene49443:CDS 35.0%
! GCATTGGAAGTTGATTTATG+AGG - chr1.1:68357151-68357170 None:intergenic 35.0%
! TAGAAGACTTTTGTGTTGAG+TGG - chr1.1:68355374-68355393 None:intergenic 35.0%
! TCTTTTGCATCAAGCATACA+GGG + chr1.1:68357263-68357282 MS.gene49443:CDS 35.0%
! TGAATCTAAGCACTTTTCAG+AGG - chr1.1:68355309-68355328 None:intergenic 35.0%
! TTCTTTTGCATCAAGCATAC+AGG + chr1.1:68357262-68357281 MS.gene49443:CDS 35.0%
! TTGGTAACTTGTCATTGTCA+AGG + chr1.1:68357369-68357388 MS.gene49443:intron 35.0%
! TTTTCTGTTCCAACAAGATC+AGG - chr1.1:68357740-68357759 None:intergenic 35.0%
!! AGGATTCTTCTAGGATTAGT+TGG + chr1.1:68356263-68356282 MS.gene49443:CDS 35.0%
!! ATTGATGGTGATGATGATGA+TGG - chr1.1:68357173-68357192 None:intergenic 35.0%
!! CTTTTCAGAGGTTTTTTCCA+TGG - chr1.1:68355297-68355316 None:intergenic 35.0%
!! GTGTTTTTGAGAGCAGAAAA+TGG + chr1.1:68355884-68355903 MS.gene49443:CDS 35.0%
!! TGAGACTATTTTTCCTAGGT+TGG + chr1.1:68355697-68355716 MS.gene49443:CDS 35.0%
!! TGTTGTAGTAGCTTGTTGAA+GGG - chr1.1:68357338-68357357 None:intergenic 35.0%
!! TTGTCTTGTTTGTTGAGTGT+GGG + chr1.1:68357479-68357498 MS.gene49443:intron 35.0%
!! TTGTTGTAGTAGCTTGTTGA+AGG - chr1.1:68357339-68357358 None:intergenic 35.0%
!!! CAAAATGCCTTTTGAAACTG+AGG + chr1.1:68356243-68356262 MS.gene49443:CDS 35.0%
AACCGTGTATCACCTTTGTT+TGG + chr1.1:68355992-68356011 MS.gene49443:CDS 40.0%
AACCTTGGTTTCTTTCTTGG+AGG - chr1.1:68357107-68357126 None:intergenic 40.0%
ACGTAGACTTCATCAGTTTC+AGG - chr1.1:68355506-68355525 None:intergenic 40.0%
AGAGCAAAGAAAGGTGGAAT+TGG + chr1.1:68355929-68355948 MS.gene49443:CDS 40.0%
ATAAGGAGAGCAAAGAAAGG+TGG + chr1.1:68355923-68355942 MS.gene49443:CDS 40.0%
ATACTCTTGTAGGGTCCATA+AGG - chr1.1:68357425-68357444 None:intergenic 40.0%
ATAGTCTCAGCACAGTATCT+AGG - chr1.1:68355686-68355705 None:intergenic 40.0%
CAATCTGATGCAAACAATGG+TGG + chr1.1:68355647-68355666 MS.gene49443:CDS 40.0%
CACCAAACAAAGGTGATACA+CGG - chr1.1:68355997-68356016 None:intergenic 40.0%
CCAGTTGAGAATTCTTCTGA+TGG + chr1.1:68357680-68357699 MS.gene49443:CDS 40.0%
CCATCAGAAGAATTCTCAAC+TGG - chr1.1:68357683-68357702 None:intergenic 40.0%
CGTAGACTTCATCAGTTTCA+GGG - chr1.1:68355505-68355524 None:intergenic 40.0%
GCAATAATTGTCTGAACAGG+TGG - chr1.1:68355737-68355756 None:intergenic 40.0%
GGTATAAGGAGAGCAAAGAA+AGG + chr1.1:68355920-68355939 MS.gene49443:CDS 40.0%
GTTGAGAATTCTTCTGATGG+AGG + chr1.1:68357683-68357702 MS.gene49443:CDS 40.0%
GTTGTCAAAAGATGTCTCCT+TGG - chr1.1:68355812-68355831 None:intergenic 40.0%
TCTGCTGAATAATCCAACCT+AGG - chr1.1:68355713-68355732 None:intergenic 40.0%
TTGTTGAGTGTGGGAAATTC+TGG + chr1.1:68357488-68357507 MS.gene49443:intron 40.0%
! AAGACTTTTGTGTTGAGTGG+TGG - chr1.1:68355371-68355390 None:intergenic 40.0%
! AGGAGACATCTTTTGACAAC+TGG + chr1.1:68355812-68355831 MS.gene49443:CDS 40.0%
! GACATCTTTTGACAACTGGT+TGG + chr1.1:68355816-68355835 MS.gene49443:CDS 40.0%
! GCTGTAAACTGTGCTGTTAA+TGG + chr1.1:68356109-68356128 MS.gene49443:CDS 40.0%
! GGGTGCTTTTGCTTCAATAT+TGG - chr1.1:68357531-68357550 None:intergenic 40.0%
! TCATGTTTTCCAGGTAGTGT+GGG + chr1.1:68356986-68357005 MS.gene49443:intron 40.0%
! TTCTTTCTTGGAGGTGTGAA+TGG - chr1.1:68357098-68357117 None:intergenic 40.0%
!! AAGAGTGGTTCTGTTTCTGA+AGG + chr1.1:68357620-68357639 MS.gene49443:intron 40.0%
!! AATCACAAGAAGCTTGTTGC+TGG + chr1.1:68355851-68355870 MS.gene49443:CDS 40.0%
!! AATTCAGGAGTACTAGCTCT+TGG - chr1.1:68356160-68356179 None:intergenic 40.0%
!! CTTGTCTTGTTTGTTGAGTG+TGG + chr1.1:68357478-68357497 MS.gene49443:intron 40.0%
!! GTGCTGAGACTATTTTTCCT+AGG + chr1.1:68355693-68355712 MS.gene49443:CDS 40.0%
AAGAAAGGTGGAATTGGTGG+TGG + chr1.1:68355935-68355954 MS.gene49443:CDS 45.0%
AGGATATGCATGGACAGTGT+TGG + chr1.1:68355759-68355778 MS.gene49443:CDS 45.0%
ATTCTACAAGGGATGAGAGG+TGG - chr1.1:68355458-68355477 None:intergenic 45.0%
CAACACAAGCCTGATCTTGT+TGG + chr1.1:68357728-68357747 MS.gene49443:CDS 45.0%
CACCTCCAAGAAAGAAACCA+AGG + chr1.1:68357102-68357121 MS.gene49443:CDS 45.0%
CCATAAGGAGGCCTAGTTAT+AGG - chr1.1:68357410-68357429 None:intergenic 45.0%
CCTATAACTAGGCCTCCTTA+TGG + chr1.1:68357407-68357426 MS.gene49443:intron 45.0%
GAGATTCTACAAGGGATGAG+AGG - chr1.1:68355461-68355480 None:intergenic 45.0%
GCAAAGAAAGGTGGAATTGG+TGG + chr1.1:68355932-68355951 MS.gene49443:CDS 45.0%
TCTGATGCAAACAATGGTGG+TGG + chr1.1:68355650-68355669 MS.gene49443:CDS 45.0%
! CGTGTATCACCTTTGTTTGG+TGG + chr1.1:68355995-68356014 MS.gene49443:CDS 45.0%
! GTATCACCTTTGTTTGGTGG+TGG + chr1.1:68355998-68356017 MS.gene49443:CDS 45.0%
! GTCATGTTTTCCAGGTAGTG+TGG + chr1.1:68356985-68357004 MS.gene49443:intron 45.0%
!! GGTGATGATGATGATGGCAT+TGG - chr1.1:68357167-68357186 None:intergenic 45.0%
AAGGGATGAGAGGTGGAACA+CGG - chr1.1:68355451-68355470 None:intergenic 50.0%
CTCTTGTAGGGTCCATAAGG+AGG - chr1.1:68357422-68357441 None:intergenic 50.0%
GAACCACCACCACCAAACAA+AGG - chr1.1:68356007-68356026 None:intergenic 50.0%
GAGTGGTGGCATTTGAACCA+TGG - chr1.1:68355357-68355376 None:intergenic 50.0%
GCATGTTCTGCATGACCTTG+TGG - chr1.1:68355401-68355420 None:intergenic 50.0%
GGAGAATCAGGCCAACGAAT+TGG - chr1.1:68356325-68356344 None:intergenic 50.0%
TGGAGGCTCTCCTTGGTATA+AGG + chr1.1:68357700-68357719 MS.gene49443:CDS 50.0%
TGGTCCAAATTGAGCATGCC+TGG - chr1.1:68357291-68357310 None:intergenic 50.0%
! TTCGTTGGCCTGATTCTCCT+TGG + chr1.1:68356326-68356345 MS.gene49443:CDS 50.0%
!! GGTGGTTCTGGTTTCTTGTG+TGG + chr1.1:68356019-68356038 MS.gene49443:CDS 50.0%
!! TCACCTTTGTTTGGTGGTGG+TGG + chr1.1:68356001-68356020 MS.gene49443:CDS 50.0%
!! TTGTTTGGTGGTGGTGGTTC+TGG + chr1.1:68356007-68356026 MS.gene49443:CDS 50.0%
AGGGATGAGAGGTGGAACAC+GGG - chr1.1:68355450-68355469 None:intergenic 55.0%
CAACTATGGCACGCATGTGC+TGG + chr1.1:68355329-68355348 MS.gene49443:CDS 55.0%
CATCAAGCATACAGGGAGCC+AGG + chr1.1:68357270-68357289 MS.gene49443:CDS 55.0%
CTGGTTCATCCCACACTACC+TGG - chr1.1:68356998-68357017 None:intergenic 55.0%
CTTCTGATGGAGGCTCTCCT+TGG + chr1.1:68357693-68357712 MS.gene49443:CDS 55.0%
GGAGCCAGGCATGCTCAATT+TGG + chr1.1:68357284-68357303 MS.gene49443:CDS 55.0%
TTCTCCTTGGCGCCTTCTTC+AGG + chr1.1:68356339-68356358 MS.gene49443:CDS 55.0%
! GAGAGGTGGAACACGGGTTT+TGG - chr1.1:68355444-68355463 None:intergenic 55.0%
!! AGAAGGCGCCAAGGAGAATC+AGG - chr1.1:68356337-68356356 None:intergenic 55.0%
!! GTTACCTGAAGAAGGCGCCA+AGG - chr1.1:68356346-68356365 None:intergenic 55.0%
! GCACGCATGTGCTGGTGCCA+TGG + chr1.1:68355337-68355356 MS.gene49443:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 68355275 68357787 68355275 ID=MS.gene49443
chr1.1 mRNA 68355275 68357787 68355275 ID=MS.gene49443.t1;Parent=MS.gene49443
chr1.1 exon 68355275 68356360 68355275 ID=MS.gene49443.t1.exon1;Parent=MS.gene49443.t1
chr1.1 CDS 68355275 68356360 68355275 ID=cds.MS.gene49443.t1;Parent=MS.gene49443.t1
chr1.1 exon 68356999 68357123 68356999 ID=MS.gene49443.t1.exon2;Parent=MS.gene49443.t1
chr1.1 CDS 68356999 68357123 68356999 ID=cds.MS.gene49443.t1;Parent=MS.gene49443.t1
chr1.1 exon 68357238 68357371 68357238 ID=MS.gene49443.t1.exon3;Parent=MS.gene49443.t1
chr1.1 CDS 68357238 68357371 68357238 ID=cds.MS.gene49443.t1;Parent=MS.gene49443.t1
chr1.1 exon 68357642 68357787 68357642 ID=MS.gene49443.t1.exon4;Parent=MS.gene49443.t1
chr1.1 CDS 68357642 68357787 68357642 ID=cds.MS.gene49443.t1;Parent=MS.gene49443.t1
Gene Sequence

>MS.gene49443

ATGTTTGTAGTTATGGATTCCATGGAAAAAACCTCTGAAAAGTGCTTAGATTCACAACTATGGCACGCATGTGCTGGTGCCATGGTTCAAATGCCACCACTCAACACAAAAGTCTTCTACTTTCCACAAGGTCATGCAGAACATGCTCATAACAAAGTAGATTTTTCCAAAACCCGTGTTCCACCTCTCATCCCTTGTAGAATCTCTGCTATGAAATACATGGCAGACCCTGAAACTGATGAAGTCTACGTCAAAATGAAACTAACCCCTTTAAGAGAAAACGAGTTAGATTTTGAAGAAGATTGTTTCTTTGGAAACAATAATGGCTTAGAGGGTCAAGAAAAGCCAGCTTCTTTTGCAAAGACATTAACACAATCTGATGCAAACAATGGTGGTGGTTTTTCTGTTCCTAGATACTGTGCTGAGACTATTTTTCCTAGGTTGGATTATTCAGCAGAACCACCTGTTCAGACAATTATTGCTAAGGATATGCATGGACAGTGTTGGAAGTTTAGACATATCTATAGAGGAACACCAAGGAGACATCTTTTGACAACTGGTTGGAGTAATTTTGTGAATCACAAGAAGCTTGTTGCTGGTGATTCAATTGTGTTTTTGAGAGCAGAAAATGGAGATCTTTGTGTTGGTATAAGGAGAGCAAAGAAAGGTGGAATTGGTGGTGGAACTGATCAGTTTTCTAATTCTTCTAGTACTTGGAACCGTGTATCACCTTTGTTTGGTGGTGGTGGTTCTGGTTTCTTGTGTGGAAATGACAACAGAAAAAATGGTGGTAATGATTTGATGGGAAAAGTGGGAGCTGAATCTGTTGTTGAAGCTGTAAACTGTGCTGTTAATGGAAGATCTTTTGAGGTTGTGTATTATCCAAGAGCTAGTACTCCTGAATTTTGTGTTAAGGTTTCTTCTGTTAAATCTGCAATGCAAATTCAATGGTGTTCTGGTATGAGATTCAAAATGCCTTTTGAAACTGAGGATTCTTCTAGGATTAGTTGGTTTATGGGAACAATTTCTTCTGTTCATGTTCAAGATCCAATTCGTTGGCCTGATTCTCCTTGGCGCCTTCTTCAGGTAACAATATTATTATAACATCATTATAGATTTTTCATATTATGCATCTTTGATTGATTCTGAGTCAATTCTGACTAATAGTTAAATTGCATCTTCAACTTTTGACTATTTCTTAGACTATTTTTTTTCTATGTAAAAAGTTAGACAATCATGTACTGTTACAGTACTGTTGAATTCAGTGCATCAATAAGATGGAACAGTTTACAGTTTGTCAGATTGAGATGATTTTTCTGATACAGATTATTATTTGTTTATGACTTTATTATCTGTTGCATTTCTTATTAAATAATTACTGTAGAAAATTTTACTACTACTTACTGTGTCAGATGTGTATGACCTGTTAAACAAGAATTAAATTAAACTAAAAGATATTTGACATTTGTTGCTATAAATAGAATTGTTAATGATTTGACATTATAGTAAAGTTGCAAATAATTTCAACTATGAGTAAGTCTCATCAGTACTGACAATTGTTCCTACTAGTACTATCTACCATAGTTGCTACTTGCTAGAATTTGGTACTTCCTTGAGAGAAAGTTATTAAGTTTATACCTACTTAATTGGCTTGAAGTAATAATTTGATTAAAATAATCTAAAGATTGTTCTAAATTTCTTGTTTATGATGTCATGTTTTCCAGGTAGTGTGGGATGAACCAGATTTACTTCAAAATGTGAAATGTGTAAACCCTTGGTTAGTTGAATTAGTCTCAAACATGCCAAACTTCAATCTCTCACCATTCACACCTCCAAGAAAGAAACCAAGGTTTGTTCAAGATCCATATTTTCACCTCATAAATCAACTTCCAATGCCATCATCATCATCACCATCAATCTCCAACATTAATCTTCTCAATTATACAAAATCTTCTCTCTGTAGCATTCAAGACACTACCAATAATAGTTCTTTTGCATCAAGCATACAGGGAGCCAGGCATGCTCAATTTGGACCAAATAATAATCCATCTGATTTAATTCCCTTCAACAAGCTACTACAACAAGACATGTTTCTTGGTAACTTGTCATTGTCAAGGTTTAATCATCAACAACCTATAACTAGGCCTCCTTATGGACCCTACAAGAGTATCAATAACAATAACAACACTAAGAACAATGTTGACTTGTCTTGTTTGTTGAGTGTGGGAAATTCTGGTCAAAGTTTTAAGGAATCCAATATTGAAGCAAAAGCACCCCATAACCATAACCATATTTTGTTATTTGGAAAAATCATTCACACTGAACAAAACAGTTCAAATATTTCAAAGAGTGGTTCTGTTTCTGAAGGAACTTCTTTGAAAACATCAAATGCTTCTTCTTCTGATCCAGTTGAGAATTCTTCTGATGGAGGCTCTCCTTGGTATAAGGATCAACAACACAAGCCTGATCTTGTTGGAACAGAAAATGTTACTGCATTGTGTATGGCTTCATAA
Protein sequence

>MS.gene49443.t1

MFVVMDSMEKTSEKCLDSQLWHACAGAMVQMPPLNTKVFYFPQGHAEHAHNKVDFSKTRVPPLIPCRISAMKYMADPETDEVYVKMKLTPLRENELDFEEDCFFGNNNGLEGQEKPASFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYSAEPPVQTIIAKDMHGQCWKFRHIYRGTPRRHLLTTGWSNFVNHKKLVAGDSIVFLRAENGDLCVGIRRAKKGGIGGGTDQFSNSSSTWNRVSPLFGGGGSGFLCGNDNRKNGGNDLMGKVGAESVVEAVNCAVNGRSFEVVYYPRASTPEFCVKVSSVKSAMQIQWCSGMRFKMPFETEDSSRISWFMGTISSVHVQDPIRWPDSPWRLLQVVWDEPDLLQNVKCVNPWLVELVSNMPNFNLSPFTPPRKKPSIQDTTNNSSFASSIQGARHAQFGPNNNPSDLIPFNKLLQQDMFLGTSLKTSNASSSDPVENSSDGGSPWYKDQQHKPDLVGTENVTALCMAS