AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49587


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49587.t1 MTR_7g074790 80.899 178 16 1 57 234 1 160 2.82e-101 294
MS.gene49587.t1 MTR_5g039760 56.410 195 60 2 57 250 1 171 4.63e-69 211
MS.gene49587.t1 MTR_7g061980 60.504 119 47 0 57 175 1 119 4.27e-45 147
MS.gene49587.t1 MTR_0402s0040 39.227 181 105 4 66 243 26 204 1.36e-31 121
MS.gene49587.t1 MTR_4g102720 32.353 170 81 2 62 231 3 138 2.23e-24 97.4
MS.gene49587.t1 MTR_6g051425 26.804 194 130 5 65 248 13 204 6.11e-18 82.0
MS.gene49587.t1 MTR_0558s0020 28.169 213 130 5 58 250 31 240 6.69e-18 81.3
MS.gene49587.t1 MTR_4g069880 54.545 66 30 0 57 122 1 66 2.21e-17 74.3
MS.gene49587.t1 MTR_5g015480 35.294 153 88 4 66 215 56 200 1.65e-16 77.0
MS.gene49587.t1 MTR_1g083640 26.291 213 134 5 58 250 46 255 2.52e-16 77.0
MS.gene49587.t1 MTR_2g014100 26.667 210 131 5 58 247 39 245 3.96e-16 75.9
MS.gene49587.t1 MTR_1g019850 33.333 141 85 3 59 197 63 196 3.68e-15 72.8
MS.gene49587.t1 MTR_2g099430 41.837 98 53 3 81 175 16 112 1.46e-13 65.9
MS.gene49587.t1 MTR_2g098880 36.585 123 67 4 53 168 48 166 1.42e-12 65.9
MS.gene49587.t1 MTR_2g098880 36.585 123 67 4 53 168 60 178 1.67e-12 65.9
MS.gene49587.t1 MTR_6g022080 28.947 152 103 4 72 219 91 241 4.38e-12 64.3
MS.gene49587.t1 MTR_7g113010 36.923 130 60 4 66 190 457 569 4.41e-12 65.9
MS.gene49587.t1 MTR_8g446020 26.904 197 114 5 58 250 30 200 1.73e-11 62.8
MS.gene49587.t1 MTR_5g076070 26.708 161 115 2 68 225 55 215 1.76e-11 63.2
MS.gene49587.t1 MTR_1g070355 26.667 165 116 3 71 231 79 242 2.53e-11 63.5
MS.gene49587.t1 MTR_2g070590 36.538 104 62 3 66 166 17 119 4.05e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49587.t1 AT4G38180 34.031 191 121 4 63 249 70 259 1.39e-25 105
MS.gene49587.t1 AT4G15090 28.634 227 151 7 32 249 11 235 8.14e-16 77.0
MS.gene49587.t1 AT4G15090 28.634 227 151 7 32 249 11 235 8.14e-16 77.0
MS.gene49587.t1 AT4G15090 28.634 227 151 7 32 249 11 235 8.14e-16 77.0
MS.gene49587.t1 AT4G15090 28.634 227 151 7 32 249 11 235 8.14e-16 77.0
MS.gene49587.t1 AT4G15090 28.634 227 151 7 32 249 11 235 8.14e-16 77.0
MS.gene49587.t1 AT5G28530 26.347 167 118 2 66 227 55 221 1.02e-13 70.9
MS.gene49587.t1 AT5G28530 26.347 167 118 2 66 227 55 221 1.02e-13 70.9
MS.gene49587.t1 AT5G28530 26.347 167 118 2 66 227 55 221 1.10e-13 70.9
MS.gene49587.t1 AT5G28530 26.347 167 118 2 66 227 55 221 1.29e-13 70.5
MS.gene49587.t1 AT5G28530 26.347 167 118 2 66 227 55 221 1.39e-13 70.5

Find 46 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACACTTTCAAGTGAAAA+TGG 0.237644 5.1:+29011839 MS.gene49587:CDS
ACCTGTCAGGTACCTTCATT+TGG 0.311547 5.1:+29011546 MS.gene49587:intron
TGGTCGATGACTCTGGATTA+AGG 0.372417 5.1:+29011981 MS.gene49587:CDS
CCTGTCAGGTACCTTCATTT+GGG 0.381495 5.1:+29011547 MS.gene49587:intron
TGACTTCTTCAAATGATGAT+TGG 0.385489 5.1:+29011579 MS.gene49587:CDS
TCGTCCGTCGCTTGATAGTC+TGG 0.388661 5.1:+29011031 MS.gene49587:CDS
GGAAGGAATACGGAAGAAAA+AGG 0.394338 5.1:+29011754 MS.gene49587:CDS
TCAAATTTCATTCCATGTCT+TGG 0.397028 5.1:-29011605 None:intergenic
CCTCCGATGACCACCTCTGC+CGG 0.401152 5.1:+29011007 MS.gene49587:CDS
TTCAAGTGAAAATGGAAAGT+TGG 0.416461 5.1:+29011847 MS.gene49587:CDS
ATGTAGGTTTGTTTGTTGTA+AGG 0.436082 5.1:+29011733 MS.gene49587:CDS
TATGCTTTACGTGAGGGCTT+CGG 0.441084 5.1:+29011662 MS.gene49587:CDS
TTAGAGTACGCTTTACAAAC+AGG 0.450669 5.1:+29011687 MS.gene49587:CDS
TCTCTCGATTTAGTAGATCA+TGG 0.467068 5.1:-29011898 None:intergenic
CTCCGATGACCACCTCTGCC+GGG 0.481857 5.1:+29011008 MS.gene49587:CDS
ATTGATATGGTCGATGACTC+TGG 0.485306 5.1:+29011974 MS.gene49587:CDS
AGGTTTGTTTGTTGTAAGGA+AGG 0.486878 5.1:+29011737 MS.gene49587:CDS
TTTGTTGTAAGGAAGGAATA+CGG 0.490669 5.1:+29011744 MS.gene49587:CDS
ACGTTAGTGTATCGTCATGT+AGG 0.496645 5.1:+29011717 MS.gene49587:CDS
CTTGCTTATGCTTTACGTGA+GGG 0.500228 5.1:+29011656 MS.gene49587:CDS
AGACAAAGAAGTATGGTGTA+TGG 0.509015 5.1:+29012100 MS.gene49587:CDS
ACAAGCATATGAGATTGATA+TGG 0.513242 5.1:+29011961 MS.gene49587:CDS
AAGGATAAATGTGCATATGA+AGG 0.516778 5.1:+29011773 MS.gene49587:CDS
CTCTCGATTTAGTAGATCAT+GGG 0.519266 5.1:-29011897 None:intergenic
CATGTCAAATACCCAAATGA+AGG 0.538422 5.1:-29011558 None:intergenic
AGCGACGGACGACCCGGCAG+AGG 0.541245 5.1:-29011020 None:intergenic
CTATCAAGCGACGGACGACC+CGG 0.546857 5.1:-29011026 None:intergenic
CAACTTATGAGCACACATGC+AGG 0.548940 5.1:+29012019 MS.gene49587:CDS
TCTTGCTTATGCTTTACGTG+AGG 0.552494 5.1:+29011655 MS.gene49587:CDS
GATTTAGTAGATCATGGGTA+TGG 0.554426 5.1:-29011892 None:intergenic
GAAATTTGACACTAGAGAAG+AGG 0.556802 5.1:+29011619 MS.gene49587:CDS
GACGGACGACCCGGCAGAGG+TGG 0.564168 5.1:-29011017 None:intergenic
GCAGGGCATAGCGCTAATGT+TGG 0.568818 5.1:+29012037 MS.gene49587:CDS
GACCCGGCAGAGGTGGTCAT+CGG 0.580561 5.1:-29011010 None:intergenic
GATGATTGGAAGCCAAGACA+TGG 0.587035 5.1:+29011593 MS.gene49587:CDS
TCACCGCTGTTGCTTTGAAG+TGG 0.594928 5.1:-29010954 None:intergenic
AACTTATGAGCACACATGCA+GGG 0.596381 5.1:+29012020 MS.gene49587:CDS
GAACGCATGAAAACTAGTTG+AGG 0.616042 5.1:-29010983 None:intergenic
CGCTAATGTTGGATTTACAG+AGG 0.620951 5.1:+29012048 MS.gene49587:CDS
CCGGCAGAGGTGGTCATCGG+AGG 0.621382 5.1:-29011007 None:intergenic
TGACTCTGGATTAAGGCAGA+AGG 0.626414 5.1:+29011988 MS.gene49587:CDS
AGTATGGTGTATGGTGACGC+TGG 0.653676 5.1:+29012109 MS.gene49587:CDS
TAATGTTGGATTTACAGAGG+TGG 0.672534 5.1:+29012051 MS.gene49587:CDS
TTGCCACTTCAAAGCAACAG+CGG 0.681368 5.1:+29010951 MS.gene49587:CDS
GATGCCAGACTATCAAGCGA+CGG 0.699838 5.1:-29011035 None:intergenic
GAAGTGGCAAGAAGAAAGAG+CGG 0.703587 5.1:-29010938 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAGATTTTAAGAAATTTA+GGG + chr5.1:29011220-29011239 MS.gene49587:intron 10.0%
!! ACCAAAATCAAAATTAAAAT+AGG + chr5.1:29011136-29011155 MS.gene49587:intron 15.0%
!!! GATTAGATTTTAAGAAATTT+AGG + chr5.1:29011219-29011238 MS.gene49587:intron 15.0%
!!! TCCTATTTTAATTTTGATTT+TGG - chr5.1:29011140-29011159 None:intergenic 15.0%
!!! ATGATGGAAAAAAAAAGATA+AGG - chr5.1:29011081-29011100 None:intergenic 20.0%
!!! GAAATTTAGGGTTTTATTTT+AGG + chr5.1:29011232-29011251 MS.gene49587:intron 20.0%
! AAATAAAAGAGGTAAGAACA+AGG - chr5.1:29011526-29011545 None:intergenic 25.0%
! AGCTAAACATTAGATGAATT+AGG + chr5.1:29011326-29011345 MS.gene49587:intron 25.0%
! CATAACTATTCAAAAGATGA+TGG - chr5.1:29011097-29011116 None:intergenic 25.0%
! TAATCCTACAGAATTGAAAT+TGG - chr5.1:29011204-29011223 None:intergenic 25.0%
! TACAAAAAGACAAAGAAGTA+TGG + chr5.1:29012093-29012112 MS.gene49587:CDS 25.0%
! TTAACAATACATGCAAATGA+AGG - chr5.1:29011487-29011506 None:intergenic 25.0%
!! AGAATTGAAATTGGTTCAAA+AGG - chr5.1:29011195-29011214 None:intergenic 25.0%
!! ATTACACTTTCAAGTGAAAA+TGG + chr5.1:29011839-29011858 MS.gene49587:CDS 25.0%
!! CATCATCTTTTGAATAGTTA+TGG + chr5.1:29011095-29011114 MS.gene49587:intron 25.0%
!!! GTAAATATGCTCTGTTTTTT+AGG + chr5.1:29011279-29011298 MS.gene49587:intron 25.0%
AAGGATAAATGTGCATATGA+AGG + chr5.1:29011773-29011792 MS.gene49587:CDS 30.0%
ACAAGCATATGAGATTGATA+TGG + chr5.1:29011961-29011980 MS.gene49587:CDS 30.0%
GGAATAACTACACTTGATTA+AGG + chr5.1:29011157-29011176 MS.gene49587:intron 30.0%
GTGCATATGAAGGAAAAATT+CGG + chr5.1:29011783-29011802 MS.gene49587:CDS 30.0%
TCAAATTTCATTCCATGTCT+TGG - chr5.1:29011608-29011627 None:intergenic 30.0%
TGAACCAATTTCAATTCTGT+AGG + chr5.1:29011197-29011216 MS.gene49587:intron 30.0%
TGACTTCTTCAAATGATGAT+TGG + chr5.1:29011579-29011598 MS.gene49587:CDS 30.0%
TTCAAGTGAAAATGGAAAGT+TGG + chr5.1:29011847-29011866 MS.gene49587:CDS 30.0%
! TTTGTTGTAAGGAAGGAATA+CGG + chr5.1:29011744-29011763 MS.gene49587:CDS 30.0%
!! ATGTAGGTTTGTTTGTTGTA+AGG + chr5.1:29011733-29011752 MS.gene49587:CDS 30.0%
ACCTGACAGGTAAATAAAAG+AGG - chr5.1:29011537-29011556 None:intergenic 35.0%
ATTGAAGTAAACCCATTGCA+AGG + chr5.1:29011255-29011274 MS.gene49587:intron 35.0%
CATGTCAAATACCCAAATGA+AGG - chr5.1:29011561-29011580 None:intergenic 35.0%
CTCTCGATTTAGTAGATCAT+GGG - chr5.1:29011900-29011919 None:intergenic 35.0%
GAAATTTGACACTAGAGAAG+AGG + chr5.1:29011619-29011638 MS.gene49587:CDS 35.0%
GATTTAGTAGATCATGGGTA+TGG - chr5.1:29011895-29011914 None:intergenic 35.0%
TAATGTTGGATTTACAGAGG+TGG + chr5.1:29012051-29012070 MS.gene49587:CDS 35.0%
TATGAAGGAAAAATTCGGAG+AGG + chr5.1:29011788-29011807 MS.gene49587:CDS 35.0%
TCTCTCGATTTAGTAGATCA+TGG - chr5.1:29011901-29011920 None:intergenic 35.0%
TTAGAGTACGCTTTACAAAC+AGG + chr5.1:29011687-29011706 MS.gene49587:CDS 35.0%
TTGAAGTAAACCCATTGCAA+GGG + chr5.1:29011256-29011275 MS.gene49587:intron 35.0%
! AGACAAAGAAGTATGGTGTA+TGG + chr5.1:29012100-29012119 MS.gene49587:CDS 35.0%
!! ACCTCTTTTATTTACCTGTC+AGG + chr5.1:29011533-29011552 MS.gene49587:intron 35.0%
!! AGGTTTGTTTGTTGTAAGGA+AGG + chr5.1:29011737-29011756 MS.gene49587:CDS 35.0%
AACTTATGAGCACACATGCA+GGG + chr5.1:29012020-29012039 MS.gene49587:CDS 40.0%
ACGTTAGTGTATCGTCATGT+AGG + chr5.1:29011717-29011736 MS.gene49587:CDS 40.0%
AGCATATTTACCCCTTGCAA+TGG - chr5.1:29011270-29011289 None:intergenic 40.0%
ATTGATATGGTCGATGACTC+TGG + chr5.1:29011974-29011993 MS.gene49587:CDS 40.0%
ATTTGGTCTGATGTCTGAGT+AGG + chr5.1:29011459-29011478 MS.gene49587:intron 40.0%
CGCTAATGTTGGATTTACAG+AGG + chr5.1:29012048-29012067 MS.gene49587:CDS 40.0%
CTGTTTGTCTGTCATATCAG+TGG + chr5.1:29011425-29011444 MS.gene49587:intron 40.0%
CTTGCTTATGCTTTACGTGA+GGG + chr5.1:29011656-29011675 MS.gene49587:CDS 40.0%
GAACGCATGAAAACTAGTTG+AGG - chr5.1:29010986-29011005 None:intergenic 40.0%
GCATATTTACCCCTTGCAAT+GGG - chr5.1:29011269-29011288 None:intergenic 40.0%
GGAAGGAATACGGAAGAAAA+AGG + chr5.1:29011754-29011773 MS.gene49587:CDS 40.0%
TCTTGCTTATGCTTTACGTG+AGG + chr5.1:29011655-29011674 MS.gene49587:CDS 40.0%
TGAAGTAAACCCATTGCAAG+GGG + chr5.1:29011257-29011276 MS.gene49587:intron 40.0%
TTTGTCTGTCATATCAGTGG+TGG + chr5.1:29011428-29011447 MS.gene49587:intron 40.0%
! GCAGGTTAGAACTTCACTTT+TGG + chr5.1:29011382-29011401 MS.gene49587:intron 40.0%
!! AACAAGGAGTGTCACTAAAC+AGG - chr5.1:29011510-29011529 None:intergenic 40.0%
ACCTGTCAGGTACCTTCATT+TGG + chr5.1:29011546-29011565 MS.gene49587:intron 45.0%
CAACTTATGAGCACACATGC+AGG + chr5.1:29012019-29012038 MS.gene49587:CDS 45.0%
CCTGTCAGGTACCTTCATTT+GGG + chr5.1:29011547-29011566 MS.gene49587:intron 45.0%
GAAGTGGCAAGAAGAAAGAG+CGG - chr5.1:29010941-29010960 None:intergenic 45.0%
GATGATTGGAAGCCAAGACA+TGG + chr5.1:29011593-29011612 MS.gene49587:CDS 45.0%
TATGCTTTACGTGAGGGCTT+CGG + chr5.1:29011662-29011681 MS.gene49587:CDS 45.0%
TGACTCTGGATTAAGGCAGA+AGG + chr5.1:29011988-29012007 MS.gene49587:CDS 45.0%
TGGTCGATGACTCTGGATTA+AGG + chr5.1:29011981-29012000 MS.gene49587:CDS 45.0%
TTGCGTGTTATCGAGTATGC+AGG + chr5.1:29011364-29011383 MS.gene49587:intron 45.0%
! CTTGATAGTCTGGCATCGTA+AGG + chr5.1:29011041-29011060 MS.gene49587:intron 45.0%
! TTGCCACTTCAAAGCAACAG+CGG + chr5.1:29010951-29010970 MS.gene49587:CDS 45.0%
!! CAGTGGTGGTGGCTTAAATT+TGG + chr5.1:29011442-29011461 MS.gene49587:intron 45.0%
GATGCCAGACTATCAAGCGA+CGG - chr5.1:29011038-29011057 None:intergenic 50.0%
GTCTGTCATATCAGTGGTGG+TGG + chr5.1:29011431-29011450 MS.gene49587:intron 50.0%
! AGTATGGTGTATGGTGACGC+TGG + chr5.1:29012109-29012128 MS.gene49587:CDS 50.0%
!! CCCAAATGAAGGTACCTGAC+AGG - chr5.1:29011550-29011569 None:intergenic 50.0%
!! TCACCGCTGTTGCTTTGAAG+TGG - chr5.1:29010957-29010976 None:intergenic 50.0%
GCAGGGCATAGCGCTAATGT+TGG + chr5.1:29012037-29012056 MS.gene49587:CDS 55.0%
! TCGTCCGTCGCTTGATAGTC+TGG + chr5.1:29011031-29011050 MS.gene49587:CDS 55.0%
CTATCAAGCGACGGACGACC+CGG - chr5.1:29011029-29011048 None:intergenic 60.0%
CCTCCGATGACCACCTCTGC+CGG + chr5.1:29011007-29011026 MS.gene49587:CDS 65.0%
CTCCGATGACCACCTCTGCC+GGG + chr5.1:29011008-29011027 MS.gene49587:CDS 65.0%
GACCCGGCAGAGGTGGTCAT+CGG - chr5.1:29011013-29011032 None:intergenic 65.0%
CCGGCAGAGGTGGTCATCGG+AGG - chr5.1:29011010-29011029 None:intergenic 70.0%
AGCGACGGACGACCCGGCAG+AGG - chr5.1:29011023-29011042 None:intergenic 75.0%
GACGGACGACCCGGCAGAGG+TGG - chr5.1:29011020-29011039 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 29010913 29012162 29010913 ID=MS.gene49587
chr5.1 mRNA 29010913 29012162 29010913 ID=MS.gene49587.t1;Parent=MS.gene49587
chr5.1 exon 29010913 29011057 29010913 ID=MS.gene49587.t1.exon1;Parent=MS.gene49587.t1
chr5.1 CDS 29010913 29011057 29010913 ID=cds.MS.gene49587.t1;Parent=MS.gene49587.t1
chr5.1 exon 29011555 29012162 29011555 ID=MS.gene49587.t1.exon2;Parent=MS.gene49587.t1
chr5.1 CDS 29011555 29012162 29011555 ID=cds.MS.gene49587.t1;Parent=MS.gene49587.t1
Gene Sequence

>MS.gene49587

ATGCAGAAAAATACAAACACGATTGCCGCTCTTTCTTCTTGCCACTTCAAAGCAACAGCGGTGACACTCACCTCAACTAGTTTTCATGCGTTCGCCTCCGATGACCACCTCTGCCGGGTCGTCCGTCGCTTGATAGTCTGGCATCGTAAGGTTCGTTTCTTTCTTCCTTATCTTTTTTTTTCCATCATCTTTTGAATAGTTATGGTCATGAGAACAAACAAAAACCAAAATCAAAATTAAAATAGGAATAACTACACTTGATTAAGGAAAGAAAACAAACCTTTTGAACCAATTTCAATTCTGTAGGATTAGATTTTAAGAAATTTAGGGTTTTATTTTAGGATTGAAGTAAACCCATTGCAAGGGGTAAATATGCTCTGTTTTTTAGGAGCAATACATGTGCTCTGTTTAATAGCTAAACATTAGATGAATTAGGTTTGTGTAGCTTTTGTTGCGTGTTATCGAGTATGCAGGTTAGAACTTCACTTTTGGAGCTGTTGTGTTTTTTATTTCTGTTTGTCTGTCATATCAGTGGTGGTGGCTTAAATTTGGTCTGATGTCTGAGTAGGTACCTTCATTTGCATGTATTGTTAACCTGTTTAGTGACACTCCTTGTTCTTACCTCTTTTATTTACCTGTCAGGTACCTTCATTTGGGTATTTGACATGACTTCTTCAAATGATGATTGGAAGCCAAGACATGGAATGAAATTTGACACTAGAGAAGAGGCTGAACAATTTTATCTTGCTTATGCTTTACGTGAGGGCTTCGGAGTTAGAGTACGCTTTACAAACAGGAAGAAAGACGTTAGTGTATCGTCATGTAGGTTTGTTTGTTGTAAGGAAGGAATACGGAAGAAAAAGGATAAATGTGCATATGAAGGAAAAATTCGGAGAGGCGAGACTAGAGCGAAATGCATAGCAAGAATTACACTTTCAAGTGAAAATGGAAAGTTGGTTATCAATAAGTTTGTAGCTAACCATACCCATGATCTACTAAATCGAGAGACAACGCACATGCTTCGATCACATAGAAAGATAACTGAAGTACAAGCATATGAGATTGATATGGTCGATGACTCTGGATTAAGGCAGAAGGAAGTGTATCAACTTATGAGCACACATGCAGGGCATAGCGCTAATGTTGGATTTACAGAGGTGGATGTAAGAAACTACATCACTACAAAAAGACAAAGAAGTATGGTGTATGGTGACGCTGGATATCTTTCACAGTATTTTCAACGACAGTAA

Protein sequence

>MS.gene49587.t1

MQKNTNTIAALSSCHFKATAVTLTSTSFHAFASDDHLCRVVRRLIVWHRTFIWVFDMTSSNDDWKPRHGMKFDTREEAEQFYLAYALREGFGVRVRFTNRKKDVSVSSCRFVCCKEGIRKKKDKCAYEGKIRRGETRAKCIARITLSSENGKLVINKFVANHTHDLLNRETTHMLRSHRKITEVQAYEIDMVDDSGLRQKEVYQLMSTHAGHSANVGFTEVDVRNYITTKRQRSMVYGDAGYLSQYFQRQ