AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49827

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49827.t1 MTR_6g027690 78.189 486 37 7 1 479 1 424 0.0 647
MS.gene49827.t1 MTR_5g018850 34.637 179 114 3 297 474 1330 1506 4.45e-20 94.4
MS.gene49827.t1 MTR_4g132610 36.111 180 110 4 297 474 870 1046 2.47e-19 92.0
MS.gene49827.t1 MTR_8g078490 33.333 180 115 4 297 474 1076 1252 1.22e-18 89.7
MS.gene49827.t1 MTR_1g069570 31.875 160 103 3 296 452 563 719 2.77e-18 88.6
MS.gene49827.t1 MTR_7g088370 33.696 184 113 6 296 472 521 702 4.31e-18 87.8
MS.gene49827.t1 MTR_7g098390 33.540 161 99 4 296 452 519 675 9.25e-18 86.7
MS.gene49827.t1 MTR_1g080340 34.146 164 95 4 296 452 402 559 4.00e-17 84.7
MS.gene49827.t1 MTR_8g078505 33.702 181 102 6 297 470 364 533 4.11e-17 84.3
MS.gene49827.t1 MTR_5g013420 31.841 201 108 8 297 474 894 1088 2.32e-16 82.8
MS.gene49827.t1 MTR_5g013420 31.841 201 108 8 297 474 894 1088 3.48e-16 82.0
MS.gene49827.t1 MTR_3g088625 32.967 182 100 8 297 474 1301 1464 7.45e-16 81.3
MS.gene49827.t1 MTR_3g088625 32.967 182 100 8 297 474 1195 1358 7.99e-16 81.3
MS.gene49827.t1 MTR_4g010830 29.851 201 112 7 297 474 785 979 3.52e-15 79.0
MS.gene49827.t1 MTR_1g035420 26.244 221 137 6 271 466 440 659 1.13e-14 77.0
MS.gene49827.t1 MTR_1g069570 35.043 117 72 2 296 411 563 676 9.63e-14 73.9
MS.gene49827.t1 MTR_1g012890 30.769 156 90 6 323 474 226 367 1.75e-13 72.0
MS.gene49827.t1 MTR_6g059310 28.037 214 127 8 271 477 63 256 2.46e-13 71.6
MS.gene49827.t1 MTR_6g059310 28.037 214 127 8 271 477 63 256 2.51e-13 71.6
MS.gene49827.t1 MTR_1g098000 28.856 201 112 6 284 470 47 230 5.37e-13 71.2
MS.gene49827.t1 MTR_8g070070 28.019 207 123 8 271 452 551 756 1.02e-12 70.9
MS.gene49827.t1 MTR_1g098370 28.358 201 113 6 284 470 50 233 1.33e-12 70.1
MS.gene49827.t1 MTR_4g010550 27.749 191 113 5 297 474 285 463 1.38e-12 70.1
MS.gene49827.t1 MTR_1g035400 26.364 220 134 7 273 466 361 578 6.81e-12 68.2
MS.gene49827.t1 MTR_1g012910 28.387 155 93 6 324 474 530 670 7.68e-12 68.2
MS.gene49827.t1 MTR_1g012910 28.387 155 93 6 324 474 367 507 1.13e-11 67.4
MS.gene49827.t1 MTR_0071s0100 28.440 218 123 9 262 477 165 351 1.17e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49827.t1 AT3G03750 58.667 225 81 4 266 479 131 354 9.49e-79 249
MS.gene49827.t1 AT3G03750 35.833 120 68 2 28 139 28 146 1.18e-13 72.8
MS.gene49827.t1 AT3G03750 54.222 225 75 5 266 479 131 338 2.12e-67 219
MS.gene49827.t1 AT3G03750 34.109 129 72 3 28 146 28 153 9.09e-14 72.8
MS.gene49827.t1 AT1G04050 33.951 162 100 3 293 452 436 592 1.04e-19 92.8
MS.gene49827.t1 AT1G04050 33.951 162 100 3 293 452 540 696 1.97e-19 92.0
MS.gene49827.t1 AT1G04050 33.951 162 100 3 293 452 540 696 1.97e-19 92.0
MS.gene49827.t1 AT3G04380 31.902 163 97 4 296 451 252 407 3.77e-17 84.3
MS.gene49827.t1 AT3G04380 32.278 158 103 2 296 451 252 407 3.92e-17 84.0
MS.gene49827.t1 AT3G04380 32.278 158 103 2 296 451 279 434 4.23e-17 84.3
MS.gene49827.t1 AT3G04380 31.902 163 97 4 296 451 236 391 4.23e-17 84.0
MS.gene49827.t1 AT3G04380 32.278 158 103 2 296 451 279 434 4.39e-17 84.0
MS.gene49827.t1 AT3G04380 32.278 158 103 2 296 451 263 418 5.00e-17 84.0
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 458 610 2.48e-16 82.4
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 458 610 2.48e-16 82.4
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 458 610 2.48e-16 82.4
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 458 610 2.48e-16 82.4
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 438 590 3.26e-16 81.6
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 550 702 4.53e-16 81.6
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 550 702 4.53e-16 81.6
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 527 679 4.80e-16 81.3
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 527 679 4.80e-16 81.3
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 527 679 4.80e-16 81.3
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 527 679 4.80e-16 81.3
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 530 682 5.50e-16 81.3
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 530 682 5.50e-16 81.3
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 507 659 5.72e-16 81.3
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 507 659 5.72e-16 81.3
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 507 659 5.72e-16 81.3
MS.gene49827.t1 AT5G43990 31.447 159 101 3 296 452 507 659 5.72e-16 81.3
MS.gene49827.t1 AT1G73100 30.000 240 130 10 272 474 430 668 1.04e-15 80.5
MS.gene49827.t1 AT2G35160 26.809 235 134 8 256 474 581 793 7.88e-15 77.8
MS.gene49827.t1 AT2G35160 26.809 235 134 8 256 474 581 793 7.88e-15 77.8
MS.gene49827.t1 AT2G35160 26.809 235 134 8 256 474 581 793 7.88e-15 77.8
MS.gene49827.t1 AT2G35160 26.809 235 134 8 256 474 581 793 7.88e-15 77.8
MS.gene49827.t1 AT2G33290 28.054 221 128 8 273 466 435 651 9.00e-15 77.4
MS.gene49827.t1 AT2G33290 28.054 221 128 8 273 466 435 651 9.00e-15 77.4
MS.gene49827.t1 AT2G33290 28.054 221 128 8 273 466 435 651 9.00e-15 77.4
MS.gene49827.t1 AT2G33290 28.054 221 128 8 273 466 435 651 9.00e-15 77.4
MS.gene49827.t1 AT2G35160 26.809 235 134 8 256 474 545 757 9.56e-15 77.4
MS.gene49827.t1 AT4G13460 29.245 212 132 6 271 466 441 650 1.99e-14 76.3
MS.gene49827.t1 AT4G13460 29.245 212 132 6 271 466 441 650 1.99e-14 76.3
MS.gene49827.t1 AT1G76710 30.726 179 107 5 297 474 66 228 3.98e-14 75.1
MS.gene49827.t1 AT1G76710 30.726 179 107 5 297 474 66 228 3.98e-14 75.1
MS.gene49827.t1 AT1G76710 30.726 179 107 5 297 474 66 228 4.22e-14 74.7
MS.gene49827.t1 AT1G76710 30.726 179 107 5 297 474 66 228 4.77e-14 74.7
MS.gene49827.t1 AT4G30860 29.612 206 118 9 271 474 284 464 8.51e-14 73.9
MS.gene49827.t1 AT5G04940 28.033 239 133 10 273 474 433 669 9.08e-14 74.3
MS.gene49827.t1 AT5G04940 28.033 239 133 10 273 474 433 669 9.08e-14 74.3
MS.gene49827.t1 AT2G22740 30.303 198 116 7 297 474 594 789 1.03e-13 74.3
MS.gene49827.t1 AT2G22740 30.303 198 116 7 297 474 594 789 1.03e-13 74.3
MS.gene49827.t1 AT5G42400 31.724 145 79 7 332 474 1261 1387 2.92e-11 66.6
MS.gene49827.t1 AT5G42400 31.724 145 79 7 332 474 1261 1387 2.92e-11 66.6
MS.gene49827.t1 AT5G42400 31.724 145 79 7 332 474 1261 1387 2.92e-11 66.6
MS.gene49827.t1 AT5G42400 31.724 145 79 7 332 474 1261 1387 2.92e-11 66.6
MS.gene49827.t1 AT5G42400 31.724 145 79 7 332 474 1261 1387 2.92e-11 66.6
MS.gene49827.t1 AT5G42400 31.724 145 79 7 332 474 1261 1387 2.92e-11 66.6
MS.gene49827.t1 AT5G42400 31.724 145 79 7 332 474 1261 1387 2.92e-11 66.6
MS.gene49827.t1 AT5G42400 31.724 145 79 7 332 474 1261 1387 2.92e-11 66.6

Find 156 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAAGGTGTTTGTGAAAA+TGG 0.149172 6.3:+19471561 MS.gene49827:CDS
TGATGATTTGGGTGAGTTTA+TGG 0.200584 6.3:-19471323 None:intergenic
GGGTTAGATATGGTAGAATT+AGG 0.206484 6.3:-19471260 None:intergenic
AGCACCCCATGGTTGATAAT+TGG 0.206933 6.3:-19471478 None:intergenic
TTTGGTGTTGGTTGTGAAAA+TGG 0.223430 6.3:+19471876 MS.gene49827:CDS
GATGGGTCTGTGGGAGATGG+TGG 0.241518 6.3:+19471792 MS.gene49827:CDS
ATGGGGTTTGTTTGCGAATC+AGG 0.258812 6.3:+19472154 MS.gene49827:CDS
GTCAAAATGGGGTTAATGTT+AGG 0.261254 6.3:+19472099 MS.gene49827:CDS
TCATCATCGTCAACCGTTCT+AGG 0.277286 6.3:-19471936 None:intergenic
GGGTGTTGTGAGAGAGAATA+AGG 0.286416 6.3:-19471455 None:intergenic
GTGGGAGGGAGTGTGGTAAT+AGG 0.291477 6.3:+19472072 MS.gene49827:CDS
ATTGCTGCTACTCGATTTCC+TGG 0.299858 6.3:-19471172 None:intergenic
GGTAATAGGGTGAGTCAAAA+TGG 0.309553 6.3:+19472086 MS.gene49827:CDS
GGTGTGGTGGTGGGAAAGGA+TGG 0.325731 6.3:+19472135 MS.gene49827:CDS
GCACCCCATGGTTGATAATT+GGG 0.332864 6.3:-19471477 None:intergenic
TATGGAGTGATTTGCAGGTT+AGG 0.334668 6.3:-19471305 None:intergenic
TGCGACGGAGGGTGATGATT+TGG 0.335813 6.3:-19471335 None:intergenic
GTGGGTTGGGTTGTTTATGT+GGG 0.337627 6.3:+19472054 MS.gene49827:CDS
AATGGGTCAGTGGGAGATGG+TGG 0.338678 6.3:+19471579 MS.gene49827:CDS
ATGGAGTGATTTGCAGGTTA+GGG 0.339447 6.3:-19471304 None:intergenic
GAGTTTATGGAGTGATTTGC+AGG 0.342943 6.3:-19471310 None:intergenic
TGTGGGTTGGGTTGTTTATG+TGG 0.352457 6.3:+19472053 MS.gene49827:CDS
GGGTCTGTGGGAGATGGTGG+GGG 0.359874 6.3:+19471795 MS.gene49827:CDS
TGGGTCTGTGGGAGATGGTG+GGG 0.360670 6.3:+19471794 MS.gene49827:CDS
GGTTAGATATGGTAGAATTA+GGG 0.360685 6.3:-19471259 None:intergenic
TTGTTGATGGTGTTGAGAAA+TGG 0.363547 6.3:-19471396 None:intergenic
ATTGGAAGAGAGTGTGGGTT+GGG 0.372465 6.3:+19472041 MS.gene49827:CDS
TCGATCGCTCTAGGCGGAAT+TGG 0.374632 6.3:-19471834 None:intergenic
TGCAACAGCCTTTCTTGCCT+TGG 0.379800 6.3:+19474204 MS.gene49827:CDS
TCAACCGTTCTAGGCGGAAT+TGG 0.392353 6.3:-19471927 None:intergenic
CGCATGAATGATTTACAAAC+CGG 0.393320 6.3:-19474029 None:intergenic
GATGCTGGGACAGTGAGTTT+TGG 0.394382 6.3:+19471645 MS.gene49827:CDS
ATGTTAGGGTTAAAATTGTA+TGG 0.395606 6.3:+19472114 MS.gene49827:CDS
ATGGGTCTGTGGGAGATGGT+GGG 0.396576 6.3:+19471793 MS.gene49827:CDS
TTGAAGGTGTTTGTGAAAAT+GGG 0.399091 6.3:+19471562 MS.gene49827:CDS
TGGGTCAGTGGGAGATGGTG+GGG 0.401674 6.3:+19471581 MS.gene49827:CDS
GATGATGAGTCGGTGAGTTT+TGG 0.402425 6.3:+19471951 MS.gene49827:CDS
TTTGATGGGGTTGATGGTAT+TGG 0.404345 6.3:+19472023 MS.gene49827:CDS
GTGTGGTGGTGGGAAAGGAT+GGG 0.406058 6.3:+19472136 MS.gene49827:CDS
TGTGGTGGTGGGAAAGGATG+GGG 0.412443 6.3:+19472137 MS.gene49827:CDS
GTAATAGGGTGAGTCAAAAT+GGG 0.414284 6.3:+19472087 MS.gene49827:CDS
TGGGAGGGAGTGTGGTAATA+GGG 0.418711 6.3:+19472073 MS.gene49827:CDS
GTGTAGAGAAAGTAGGAGTA+AGG 0.418816 6.3:-19471426 None:intergenic
ATTCCGCCGGTTGATGAAGT+TGG 0.420784 6.3:+19471723 MS.gene49827:CDS
GGGTCAGTGGGAGATGGTGG+GGG 0.421284 6.3:+19471582 MS.gene49827:CDS
AATTCTGTCATTTCAGATGA+TGG 0.424728 6.3:+19471510 MS.gene49827:CDS
TGTTTATGTGGGAGGGAGTG+TGG 0.424996 6.3:+19472065 MS.gene49827:CDS
TTTCAAGGCGTATGTGATGA+TGG 0.425214 6.3:+19471774 MS.gene49827:CDS
AAGGTGTCAGTGATGATGGT+GGG 0.425463 6.3:+19471679 MS.gene49827:CDS
TTCCGCCGGTTGATGAAGTT+GGG 0.426029 6.3:+19471724 MS.gene49827:CDS
TGTTGATGGTGTTGAGAAAT+GGG 0.426613 6.3:-19471395 None:intergenic
TCAAAATGGGGTTAATGTTA+GGG 0.434140 6.3:+19472100 MS.gene49827:CDS
GTAAGGAGGGTTGTTGTTGA+TGG 0.435276 6.3:-19471409 None:intergenic
ACAACAACATTATGATGAAC+TGG 0.439102 6.3:+19473897 MS.gene49827:CDS
GTTGGGTTGTTTATGTGGGA+GGG 0.444408 6.3:+19472058 MS.gene49827:CDS
ACCGGCCCAACTTCATCAAC+CGG 0.447528 6.3:-19471729 None:intergenic
GATGATGGGTCGGTGAGATT+TGG 0.451262 6.3:+19471525 MS.gene49827:CDS
GAAGTTGGGCCGGTGAAGTT+TGG 0.452392 6.3:+19471738 MS.gene49827:CDS
GCGACGGAGGGTGATGATTT+GGG 0.460306 6.3:-19471334 None:intergenic
AAAATTGTATGGTGTGGTGG+TGG 0.461012 6.3:+19472125 MS.gene49827:CDS
CCACCACCTATTGATGATGC+TGG 0.461845 6.3:+19471630 MS.gene49827:CDS
AATTATCAACCATGGGGTGC+TGG 0.471919 6.3:+19471480 MS.gene49827:CDS
GCAGTGACACGGCCGCCCAT+TGG 0.475968 6.3:-19474184 None:intergenic
ACTGTCCCAGCATCATCAAT+AGG 0.476232 6.3:-19471636 None:intergenic
CTCAAACATGCCTTTCCTGA+TGG 0.478841 6.3:-19473970 None:intergenic
GATGATGGGTCTGTGGGAGA+TGG 0.479549 6.3:+19471789 MS.gene49827:CDS
GGTTGGGTTGTTTATGTGGG+AGG 0.481940 6.3:+19472057 MS.gene49827:CDS
GTAAGGGAACATCTTCCATC+AGG 0.484888 6.3:+19473955 MS.gene49827:CDS
TTGGTGTTGGTTGTGAAAAT+GGG 0.487055 6.3:+19471877 MS.gene49827:CDS
ATGGGTCAGTGGGAGATGGT+GGG 0.487685 6.3:+19471580 MS.gene49827:CDS
TAGAGAAAGTAGGAGTAAGG+AGG 0.488395 6.3:-19471423 None:intergenic
GAGTTCTTGTTTCAATATGC+AGG 0.491591 6.3:+19472191 MS.gene49827:CDS
GATGAGGGATCAGTGAGATT+TGG 0.492622 6.3:+19471858 MS.gene49827:CDS
GGAAGGTGTGTAGAGAAAGT+AGG 0.494404 6.3:-19471433 None:intergenic
CAACCATGGGGTGCTGGAGA+AGG 0.499009 6.3:+19471486 MS.gene49827:CDS
TGGGAAGGTGTCAGTGATGA+TGG 0.503731 6.3:+19471675 MS.gene49827:CDS
GTATGGTGTGGTGGTGGGAA+AGG 0.504197 6.3:+19472131 MS.gene49827:CDS
CTCAAAGGTTCGCGAAGCGT+CGG 0.508842 6.3:-19471361 None:intergenic
AGAGAAAGTAGGAGTAAGGA+GGG 0.510073 6.3:-19471422 None:intergenic
AACTTTCAAGTGTCTTCTGA+GGG 0.512350 6.3:-19474234 None:intergenic
CAACTTTCAAGTGTCTTCTG+AGG 0.513305 6.3:-19474235 None:intergenic
GCAATGTTCCAAGGCAAGAA+AGG 0.514478 6.3:-19474212 None:intergenic
TCAATAGGTGGTGGTGTTAG+TGG 0.514673 6.3:-19471621 None:intergenic
GAATCAGGTTGTTCGTAAAG+AGG 0.515528 6.3:+19472169 MS.gene49827:CDS
GCCGGTTGATGAAGTTGGGC+CGG 0.518021 6.3:+19471728 MS.gene49827:CDS
GAAAATGGGAAGGTGGGAGG+TGG 0.524391 6.3:+19471891 MS.gene49827:CDS
AGTTGTGTGTGGGTTAGATA+TGG 0.525928 6.3:-19471270 None:intergenic
ATTCTGTCATTTCAGATGAT+GGG 0.526380 6.3:+19471511 MS.gene49827:CDS
CACCCCATGGTTGATAATTG+GGG 0.526836 6.3:-19471476 None:intergenic
AGATGTTGGCGAGTTGTGTG+TGG 0.533165 6.3:-19471281 None:intergenic
AGTAATTTCACTAATTCCGC+CGG 0.536149 6.3:+19471710 MS.gene49827:CDS
CACCACCTATTGATGATGCT+GGG 0.536926 6.3:+19471631 MS.gene49827:CDS
CCCTCATCATCGATCGCTCT+AGG 0.537573 6.3:-19471843 None:intergenic
GCCTAGAGCGATCGATGATG+AGG 0.542017 6.3:+19471842 MS.gene49827:CDS
TTTGCAGGTTAGGGAGATGT+TGG 0.542214 6.3:-19471295 None:intergenic
AAATCAGGAAAAGGTCCAAT+GGG 0.543500 6.3:+19474169 MS.gene49827:CDS
TGGTTGTGAAAATGGGAAGG+TGG 0.545226 6.3:+19471884 MS.gene49827:CDS
TTCAAGGCGTATGTGATGAT+GGG 0.548622 6.3:+19471775 MS.gene49827:CDS
GGGCCGGTGAAGTTTGGTGT+TGG 0.548634 6.3:+19471744 MS.gene49827:CDS
GGTTGTGAAAATGGGAAGGT+GGG 0.549690 6.3:+19471885 MS.gene49827:CDS
GCTATGGAGAAATCAGGAAA+AGG 0.549928 6.3:+19474160 MS.gene49827:CDS
TTTGTAAATCATTCATGCGA+TGG 0.552323 6.3:+19474033 MS.gene49827:CDS
GGGTCGGTGAGATTTGGTGT+TGG 0.554917 6.3:+19471531 MS.gene49827:CDS
TTCACCAATTCCGCCTAGAA+CGG 0.555345 6.3:+19471923 MS.gene49827:CDS
GTTAAAATTGTATGGTGTGG+TGG 0.556450 6.3:+19472122 MS.gene49827:CDS
GAAGGTGTCAGTGATGATGG+TGG 0.558181 6.3:+19471678 MS.gene49827:CDS
GAAAATGGGTCAGTGGGAGA+TGG 0.560650 6.3:+19471576 MS.gene49827:CDS
GGTGTTGTGAGAGAGAATAA+GGG 0.561729 6.3:-19471454 None:intergenic
ATGGTGTTGAGAAATGGGAT+TGG 0.561886 6.3:-19471390 None:intergenic
GGATCAGTGAGATTTGGTGT+TGG 0.566998 6.3:+19471864 MS.gene49827:CDS
GATGTTGGCGAGTTGTGTGT+GGG 0.577849 6.3:-19471280 None:intergenic
GAAAGGCTGTTGCAGTGACA+CGG 0.578224 6.3:-19474195 None:intergenic
AGGTGTCAGTGATGATGGTG+GGG 0.580529 6.3:+19471680 MS.gene49827:CDS
ATGGTATTGGAAGAGAGTGT+GGG 0.581994 6.3:+19472036 MS.gene49827:CDS
TGTGAAAATGGGAAGGTGGG+AGG 0.582238 6.3:+19471888 MS.gene49827:CDS
TATTGGAAGAGAGTGTGGGT+TGG 0.583102 6.3:+19472040 MS.gene49827:CDS
GATGGTATTGGAAGAGAGTG+TGG 0.584769 6.3:+19472035 MS.gene49827:CDS
GAAATCAGGAAAAGGTCCAA+TGG 0.584950 6.3:+19474168 MS.gene49827:CDS
CTTCTGAGGGCAATGTTCCA+AGG 0.591471 6.3:-19474221 None:intergenic
CATCCAACACCAAACTTCAC+CGG 0.591738 6.3:-19471747 None:intergenic
CCAGCATCATCAATAGGTGG+TGG 0.595249 6.3:-19471630 None:intergenic
GTAAATCATTCATGCGATGG+TGG 0.599638 6.3:+19474036 MS.gene49827:CDS
TTGTCTCCTCTGTGCTTCCG+TGG 0.600236 6.3:-19473878 None:intergenic
AGGGTTAAAATTGTATGGTG+TGG 0.603609 6.3:+19472119 MS.gene49827:CDS
AACGGTTGACGATGATGAGT+CGG 0.604298 6.3:+19471941 MS.gene49827:CDS
TTGCTGCTACTCGATTTCCT+GGG 0.607135 6.3:-19471171 None:intergenic
GGTGTGGGATGTGAGTGTGA+AGG 0.607479 6.3:+19471972 MS.gene49827:CDS
GCGAAGCGTCGGAGATGCGA+CGG 0.607942 6.3:-19471350 None:intergenic
TGGGATTGGATGATTCTCAA+AGG 0.608658 6.3:-19471376 None:intergenic
TATGATGAACTGGCATCGCG+TGG 0.618128 6.3:+19473907 MS.gene49827:CDS
GGAACATCTTCCATCAGGAA+AGG 0.618170 6.3:+19473960 MS.gene49827:CDS
TTACCTTCTCCAGCACCCCA+TGG 0.618252 6.3:-19471489 None:intergenic
AAATTGTATGGTGTGGTGGT+GGG 0.618996 6.3:+19472126 MS.gene49827:CDS
TGTCATTTCAGATGATGGGT+CGG 0.619359 6.3:+19471515 MS.gene49827:CDS
TGTTGGTTGTGAAAATGGGA+AGG 0.619618 6.3:+19471881 MS.gene49827:CDS
AAGCGTCGGAGATGCGACGG+AGG 0.619688 6.3:-19471347 None:intergenic
GTTTGTGAAAATGGGTCAGT+GGG 0.621140 6.3:+19471570 MS.gene49827:CDS
GGTGTCAGTGATGATGGTGG+GGG 0.623380 6.3:+19471681 MS.gene49827:CDS
CGTATGTGATGATGGGTCTG+TGG 0.626173 6.3:+19471782 MS.gene49827:CDS
AGCGTCGGAGATGCGACGGA+GGG 0.627600 6.3:-19471346 None:intergenic
CCTAGAGCGATCGATGATGA+GGG 0.632076 6.3:+19471843 MS.gene49827:CDS
AGGAAATCGAGTAGCAGCAA+TGG 0.633956 6.3:+19471174 MS.gene49827:CDS
CCACACAGATGAAGATACCC+AGG 0.648722 6.3:+19471154 None:intergenic
GTATGTGATGATGGGTCTGT+GGG 0.650572 6.3:+19471783 MS.gene49827:CDS
TAATAGGGTGAGTCAAAATG+GGG 0.652968 6.3:+19472088 MS.gene49827:CDS
ACCCCATGGTTGATAATTGG+GGG 0.658176 6.3:-19471475 None:intergenic
TTGTGAGAGAGAATAAGGGA+AGG 0.661426 6.3:-19471450 None:intergenic
GTCCCAGCATCATCAATAGG+TGG 0.664069 6.3:-19471633 None:intergenic
TGTTTGTGAAAATGGGTCAG+TGG 0.667809 6.3:+19471569 MS.gene49827:CDS
TCAGGAAAAGGTCCAATGGG+CGG 0.668437 6.3:+19474172 MS.gene49827:CDS
GCATGAATGATTTACAAACC+GGG 0.668682 6.3:-19474028 None:intergenic
GGCCCAACTTCATCAACCGG+CGG 0.669512 6.3:-19471726 None:intergenic
TCATCGTCAACCGTTCTAGG+CGG 0.674847 6.3:-19471933 None:intergenic
TCATCATCGATCGCTCTAGG+CGG 0.683321 6.3:-19471840 None:intergenic
AGGTGAACTACTGACAACCA+CGG 0.715721 6.3:+19473861 MS.gene49827:intron
TGACAACCACGGAAGCACAG+AGG 0.752462 6.3:+19473872 MS.gene49827:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATTTAATTTTTAGGTA+TGG + chr6.3:19472654-19472673 MS.gene49827:intron 10.0%
!!! TATGTAAAAAGTTACTTTAT+AGG + chr6.3:19473144-19473163 MS.gene49827:intron 15.0%
!!! TGTATTTAAGTTAATAGTTA+AGG + chr6.3:19473315-19473334 MS.gene49827:intron 15.0%
!! AAAACTTAATATTTCACTCA+AGG - chr6.3:19473033-19473052 None:intergenic 20.0%
!! AAACTTAATATTTCACTCAA+GGG - chr6.3:19473032-19473051 None:intergenic 20.0%
!! ATCTTAACAAGCTAATAATT+AGG - chr6.3:19473081-19473100 None:intergenic 20.0%
!! GATCAATTCTAAATTTCTAA+TGG - chr6.3:19472442-19472461 None:intergenic 20.0%
!! TCTTAACAAGCTAATAATTA+GGG - chr6.3:19473080-19473099 None:intergenic 20.0%
!! TTATCAAATATGAGTCTATT+TGG + chr6.3:19473414-19473433 MS.gene49827:intron 20.0%
!!! ATGCTTGTAAAATTAATTTG+CGG + chr6.3:19473823-19473842 MS.gene49827:intron 20.0%
!!! TAACTTAAATACAAAAGTAC+AGG - chr6.3:19473308-19473327 None:intergenic 20.0%
! ATGAAAAACCAGAAAATACT+TGG - chr6.3:19472858-19472877 None:intergenic 25.0%
! ATGTTAGGGTTAAAATTGTA+TGG + chr6.3:19472114-19472133 MS.gene49827:CDS 25.0%
! GACTAATATAGAGATTTGAA+AGG + chr6.3:19473353-19473372 MS.gene49827:intron 25.0%
! GTGATTGTTTGATTGATTAT+AGG + chr6.3:19472246-19472265 MS.gene49827:intron 25.0%
! TGATTATAGGATTGTAACAT+TGG + chr6.3:19472259-19472278 MS.gene49827:intron 25.0%
! TTTATACTATAGTAGGAACA+CGG + chr6.3:19473188-19473207 MS.gene49827:intron 25.0%
!! AATTTTACAAGCATTTGTGT+AGG - chr6.3:19473817-19473836 None:intergenic 25.0%
!! ATCAGAATTATTCACCTTTT+GGG - chr6.3:19472764-19472783 None:intergenic 25.0%
!! ATTTAGAATTGATCCGAATT+TGG + chr6.3:19472448-19472467 MS.gene49827:intron 25.0%
!! ATTTTACAAGCATTTGTGTA+GGG - chr6.3:19473816-19473835 None:intergenic 25.0%
!! CTTTAGTTCATAAGCATATA+TGG - chr6.3:19473680-19473699 None:intergenic 25.0%
!!! AGTGTATGTGTTTTTTTGAT+GGG + chr6.3:19472009-19472028 MS.gene49827:CDS 25.0%
!!! ATATTTTAGTGTTTTTGCGT+AGG + chr6.3:19472291-19472310 MS.gene49827:intron 25.0%
!!! CAAAATTTTAGCACCAAATT+CGG - chr6.3:19472464-19472483 None:intergenic 25.0%
!!! GTATTTTCTGGTTTTTCATA+CGG + chr6.3:19472859-19472878 MS.gene49827:intron 25.0%
!!! TTATTAGCTTGTTAAGATGA+TGG + chr6.3:19473083-19473102 MS.gene49827:intron 25.0%
!!! TTTGTTTTCATAAGCTGTTT+CGG - chr6.3:19473545-19473564 None:intergenic 25.0%
AACATAGACGCAACAAAAAT+AGG + chr6.3:19473997-19474016 MS.gene49827:CDS 30.0%
AATACGTTGAAAACAGCTTA+TGG - chr6.3:19473510-19473529 None:intergenic 30.0%
ACAACAACATTATGATGAAC+TGG + chr6.3:19473897-19473916 MS.gene49827:CDS 30.0%
CAAATTGCAATATGTTGAGT+AGG + chr6.3:19473254-19473273 MS.gene49827:intron 30.0%
GGTTAGATATGGTAGAATTA+GGG - chr6.3:19471262-19471281 None:intergenic 30.0%
TCAAAATGGGGTTAATGTTA+GGG + chr6.3:19472100-19472119 MS.gene49827:CDS 30.0%
TTAATATTTCACTCAAGGGA+AGG - chr6.3:19473028-19473047 None:intergenic 30.0%
TTATACTATAGTAGGAACAC+GGG + chr6.3:19473189-19473208 MS.gene49827:intron 30.0%
TTGAATTTGAACGTGGAATT+CGG + chr6.3:19472320-19472339 MS.gene49827:intron 30.0%
TTTGTAAATCATTCATGCGA+TGG + chr6.3:19474033-19474052 MS.gene49827:CDS 30.0%
! AATTCTGTCATTTCAGATGA+TGG + chr6.3:19471510-19471529 MS.gene49827:CDS 30.0%
! ATTCTGTCATTTCAGATGAT+GGG + chr6.3:19471511-19471530 MS.gene49827:CDS 30.0%
! CATCAGAATTATTCACCTTT+TGG - chr6.3:19472765-19472784 None:intergenic 30.0%
! GAAATCATAAAGTGCTTGTT+TGG + chr6.3:19472522-19472541 MS.gene49827:intron 30.0%
! TTGTTAAGATGATGGTCTAT+AGG + chr6.3:19473091-19473110 MS.gene49827:intron 30.0%
! TTTGCTATGTTAAGGTGATT+GGG + chr6.3:19473736-19473755 MS.gene49827:intron 30.0%
! TTTTGTTCTACTTGAGAATG+TGG - chr6.3:19472973-19472992 None:intergenic 30.0%
!! AATTGATTTTGCCTTCTAAG+TGG - chr6.3:19473232-19473251 None:intergenic 30.0%
!! ATTGATTTTGCCTTCTAAGT+GGG - chr6.3:19473231-19473250 None:intergenic 30.0%
!! GATTGATTTGAATTTGAACG+TGG + chr6.3:19472313-19472332 MS.gene49827:intron 30.0%
!! TTATAGGTGCTCTAAGAAAT+CGG + chr6.3:19473160-19473179 MS.gene49827:intron 30.0%
!! TTGAAGGTGTTTGTGAAAAT+GGG + chr6.3:19471562-19471581 MS.gene49827:CDS 30.0%
!! TTTGAAGGTGTTTGTGAAAA+TGG + chr6.3:19471561-19471580 MS.gene49827:CDS 30.0%
!!! GAGTGTATGTGTTTTTTTGA+TGG + chr6.3:19472008-19472027 MS.gene49827:CDS 30.0%
!!! GTGTATGTGTTTTTTTGATG+GGG + chr6.3:19472010-19472029 MS.gene49827:CDS 30.0%
!!! GTGTTTTTGTGTTTTAGAGT+TGG + chr6.3:19472406-19472425 MS.gene49827:intron 30.0%
AAATCAGGAAAAGGTCCAAT+GGG + chr6.3:19474169-19474188 MS.gene49827:CDS 35.0%
AAGAACATAACAGACCCAAA+AGG + chr6.3:19472747-19472766 MS.gene49827:intron 35.0%
ACCTTAACATAGCAAACACT+AGG - chr6.3:19473732-19473751 None:intergenic 35.0%
AGGGTTAAAATTGTATGGTG+TGG + chr6.3:19472119-19472138 MS.gene49827:CDS 35.0%
AGTAATTTCACTAATTCCGC+CGG + chr6.3:19471710-19471729 MS.gene49827:CDS 35.0%
CAAAAATAGGAAATGTTGCC+CGG + chr6.3:19474010-19474029 MS.gene49827:CDS 35.0%
CGCATGAATGATTTACAAAC+CGG - chr6.3:19474032-19474051 None:intergenic 35.0%
GAATCATACCAACAAGTTGT+TGG - chr6.3:19472900-19472919 None:intergenic 35.0%
GCATGAATGATTTACAAACC+GGG - chr6.3:19474031-19474050 None:intergenic 35.0%
GGGTTAGATATGGTAGAATT+AGG - chr6.3:19471263-19471282 None:intergenic 35.0%
GTAATAGGGTGAGTCAAAAT+GGG + chr6.3:19472087-19472106 MS.gene49827:CDS 35.0%
GTCAAAATGGGGTTAATGTT+AGG + chr6.3:19472099-19472118 MS.gene49827:CDS 35.0%
GTGCTTGTTTGGATATATGT+TGG + chr6.3:19472533-19472552 MS.gene49827:intron 35.0%
GTTAAAATTGTATGGTGTGG+TGG + chr6.3:19472122-19472141 MS.gene49827:CDS 35.0%
GTTTGGGAAACAACTTATGA+CGG + chr6.3:19473482-19473501 MS.gene49827:intron 35.0%
TAATAGGGTGAGTCAAAATG+GGG + chr6.3:19472088-19472107 MS.gene49827:CDS 35.0%
TGGCAATTGAAAATGTACAG+TGG - chr6.3:19472996-19473015 None:intergenic 35.0%
TTAACATAGCAAACACTAGG+AGG - chr6.3:19473729-19473748 None:intergenic 35.0%
TTTGGGAAACAACTTATGAC+GGG + chr6.3:19473483-19473502 MS.gene49827:intron 35.0%
! AAGCAAGCTCTTCATCTTTT+TGG - chr6.3:19474137-19474156 None:intergenic 35.0%
! GAGTTCTTGTTTCAATATGC+AGG + chr6.3:19472191-19472210 MS.gene49827:CDS 35.0%
! GTCTGCTCTTTTAGTAGTAA+GGG + chr6.3:19473939-19473958 MS.gene49827:CDS 35.0%
! TACTTGGTGATCGAATTTAC+CGG - chr6.3:19472842-19472861 None:intergenic 35.0%
! TGATGATTTGGGTGAGTTTA+TGG - chr6.3:19471326-19471345 None:intergenic 35.0%
! TTCGATCACCAAGTATTTTC+TGG + chr6.3:19472847-19472866 MS.gene49827:intron 35.0%
!! ATAAGCGCTGATGTTATAAG+CGG + chr6.3:19473627-19473646 MS.gene49827:intron 35.0%
!! CTTTTAGCTATGGAGAAATC+AGG + chr6.3:19474154-19474173 MS.gene49827:CDS 35.0%
!! GGGAGGTTTTATACTATAGT+AGG + chr6.3:19473181-19473200 MS.gene49827:intron 35.0%
!! GGTGTTGGATGTAATTTTCA+AGG + chr6.3:19471759-19471778 MS.gene49827:CDS 35.0%
!! GTTTGCTATGTTAAGGTGAT+TGG + chr6.3:19473735-19473754 MS.gene49827:intron 35.0%
!! TATAGGTGCTCTAAGAAATC+GGG + chr6.3:19473161-19473180 MS.gene49827:intron 35.0%
!! TCCTAGTGTTTGCTATGTTA+AGG + chr6.3:19473728-19473747 MS.gene49827:intron 35.0%
!! TGTTGATGGTGTTGAGAAAT+GGG - chr6.3:19471398-19471417 None:intergenic 35.0%
!! TTGATAATGTCACCATCTCA+GGG - chr6.3:19473401-19473420 None:intergenic 35.0%
!! TTGGTGTTGGTTGTGAAAAT+GGG + chr6.3:19471877-19471896 MS.gene49827:CDS 35.0%
!! TTGTTGATGGTGTTGAGAAA+TGG - chr6.3:19471399-19471418 None:intergenic 35.0%
!! TTTGATAATGTCACCATCTC+AGG - chr6.3:19473402-19473421 None:intergenic 35.0%
!! TTTGGTGTTGGTTGTGAAAA+TGG + chr6.3:19471876-19471895 MS.gene49827:CDS 35.0%
!!! AGTGAGTTTTGGTGTTGAAT+GGG + chr6.3:19471656-19471675 MS.gene49827:CDS 35.0%
!!! TGTTTTTTTGATGGGGTTGA+TGG + chr6.3:19472017-19472036 MS.gene49827:CDS 35.0%
AAAATTGTATGGTGTGGTGG+TGG + chr6.3:19472125-19472144 MS.gene49827:CDS 40.0%
AAATTGTATGGTGTGGTGGT+GGG + chr6.3:19472126-19472145 MS.gene49827:CDS 40.0%
AAGCACTTGTGAGACTGTTT+GGG + chr6.3:19473466-19473485 MS.gene49827:intron 40.0%
AATTTGAACGTGGAATTCGG+AGG + chr6.3:19472323-19472342 MS.gene49827:intron 40.0%
AGAGAAAGTAGGAGTAAGGA+GGG - chr6.3:19471425-19471444 None:intergenic 40.0%
CTTTCGAAGCAAAAAAGCAG+AGG - chr6.3:19474110-19474129 None:intergenic 40.0%
GAAATCAGGAAAAGGTCCAA+TGG + chr6.3:19474168-19474187 MS.gene49827:CDS 40.0%
GCTATGGAGAAATCAGGAAA+AGG + chr6.3:19474160-19474179 MS.gene49827:CDS 40.0%
GGTAATAGGGTGAGTCAAAA+TGG + chr6.3:19472086-19472105 MS.gene49827:CDS 40.0%
GTAAATCATTCATGCGATGG+TGG + chr6.3:19474036-19474055 MS.gene49827:CDS 40.0%
GTACAGGCAGTTTACATCTA+CGG - chr6.3:19473292-19473311 None:intergenic 40.0%
GTGTAGAGAAAGTAGGAGTA+AGG - chr6.3:19471429-19471448 None:intergenic 40.0%
GTTTGTGAAAATGGGTCAGT+GGG + chr6.3:19471570-19471589 MS.gene49827:CDS 40.0%
TAGAGAAAGTAGGAGTAAGG+AGG - chr6.3:19471426-19471445 None:intergenic 40.0%
TCAAGGGAAGGTTGTCAAAA+TGG - chr6.3:19473016-19473035 None:intergenic 40.0%
TGGGATTGGATGATTCTCAA+AGG - chr6.3:19471379-19471398 None:intergenic 40.0%
TGTTTGTGAAAATGGGTCAG+TGG + chr6.3:19471569-19471588 MS.gene49827:CDS 40.0%
TTCAAGGCGTATGTGATGAT+GGG + chr6.3:19471775-19471794 MS.gene49827:CDS 40.0%
TTGTGAGAGAGAATAAGGGA+AGG - chr6.3:19471453-19471472 None:intergenic 40.0%
TTTCAAGGCGTATGTGATGA+TGG + chr6.3:19471774-19471793 MS.gene49827:CDS 40.0%
! AGATGATGGTCTATAGGTCA+AGG + chr6.3:19473097-19473116 MS.gene49827:intron 40.0%
! ATGGAGTGATTTGCAGGTTA+GGG - chr6.3:19471307-19471326 None:intergenic 40.0%
! ATGGTATTGGAAGAGAGTGT+GGG + chr6.3:19472036-19472055 MS.gene49827:CDS 40.0%
! ATGGTGTTGAGAAATGGGAT+TGG - chr6.3:19471393-19471412 None:intergenic 40.0%
! CGTCTGCTCTTTTAGTAGTA+AGG + chr6.3:19473938-19473957 MS.gene49827:CDS 40.0%
! GAATCAGGTTGTTCGTAAAG+AGG + chr6.3:19472169-19472188 MS.gene49827:CDS 40.0%
! GAGTTTATGGAGTGATTTGC+AGG - chr6.3:19471313-19471332 None:intergenic 40.0%
! GATGATGGTCTATAGGTCAA+GGG + chr6.3:19473098-19473117 MS.gene49827:intron 40.0%
! GGTGTTGTGAGAGAGAATAA+GGG - chr6.3:19471457-19471476 None:intergenic 40.0%
! TATGGAGTGATTTGCAGGTT+AGG - chr6.3:19471308-19471327 None:intergenic 40.0%
! TGATAATGTCACCATCTCAG+GGG - chr6.3:19473400-19473419 None:intergenic 40.0%
! TGCGGTTTTCTGTGTGAAAA+AGG + chr6.3:19473841-19473860 MS.gene49827:intron 40.0%
! TGTTGGTTGTGAAAATGGGA+AGG + chr6.3:19471881-19471900 MS.gene49827:CDS 40.0%
!! AGTTGTGTGTGGGTTAGATA+TGG - chr6.3:19471273-19471292 None:intergenic 40.0%
!! GAAGAGCTTGCTTTTAGCTA+TGG + chr6.3:19474144-19474163 MS.gene49827:CDS 40.0%
!! GTTTTAGCAACCCACTTAGA+AGG + chr6.3:19473218-19473237 MS.gene49827:intron 40.0%
!! TGTCATTTCAGATGATGGGT+CGG + chr6.3:19471515-19471534 MS.gene49827:CDS 40.0%
!! TTTGATGGGGTTGATGGTAT+TGG + chr6.3:19472023-19472042 MS.gene49827:CDS 40.0%
!!! AGTTTTGGTGTTGAATGGGA+AGG + chr6.3:19471660-19471679 MS.gene49827:CDS 40.0%
!!! CAGTGAGTTTTGGTGTTGAA+TGG + chr6.3:19471655-19471674 MS.gene49827:CDS 40.0%
!!! GGTGTTGGTTGTGATTTTGA+AGG + chr6.3:19471546-19471565 MS.gene49827:CDS 40.0%
AACGGTTGACGATGATGAGT+CGG + chr6.3:19471941-19471960 MS.gene49827:CDS 45.0%
AATTATCAACCATGGGGTGC+TGG + chr6.3:19471480-19471499 MS.gene49827:CDS 45.0%
ACACCCCCAATTATCAACCA+TGG + chr6.3:19471472-19471491 MS.gene49827:CDS 45.0%
ACCCCCAATTATCAACCATG+GGG + chr6.3:19471474-19471493 MS.gene49827:CDS 45.0%
ACTGTCCCAGCATCATCAAT+AGG - chr6.3:19471639-19471658 None:intergenic 45.0%
AGATGGACCTGGATTGAACT+TGG - chr6.3:19472806-19472825 None:intergenic 45.0%
AGCTCTACCAAGTTCAATCC+AGG + chr6.3:19472796-19472815 MS.gene49827:intron 45.0%
AGGAAATCGAGTAGCAGCAA+TGG + chr6.3:19471174-19471193 MS.gene49827:CDS 45.0%
AGGTGAACTACTGACAACCA+CGG + chr6.3:19473861-19473880 MS.gene49827:intron 45.0%
AGTTCAATCCAGGTCCATCT+AGG + chr6.3:19472806-19472825 MS.gene49827:intron 45.0%
ATAGTAGGAACACGGGTCTT+TGG + chr6.3:19473196-19473215 MS.gene49827:intron 45.0%
ATTGCTGCTACTCGATTTCC+TGG - chr6.3:19471175-19471194 None:intergenic 45.0%
CAAGCACTTGTGAGACTGTT+TGG + chr6.3:19473465-19473484 MS.gene49827:intron 45.0%
CACCCCCAATTATCAACCAT+GGG + chr6.3:19471473-19471492 MS.gene49827:CDS 45.0%
CATCCAACACCAAACTTCAC+CGG - chr6.3:19471750-19471769 None:intergenic 45.0%
CCAACAAGTTGTTGGCAACA+AGG - chr6.3:19472892-19472911 None:intergenic 45.0%
CCTTGTTGCCAACAACTTGT+TGG + chr6.3:19472889-19472908 MS.gene49827:intron 45.0%
CTCAAACATGCCTTTCCTGA+TGG - chr6.3:19473973-19473992 None:intergenic 45.0%
GATGAGGGATCAGTGAGATT+TGG + chr6.3:19471858-19471877 MS.gene49827:CDS 45.0%
GCAATGTTCCAAGGCAAGAA+AGG - chr6.3:19474215-19474234 None:intergenic 45.0%
GGAACATCTTCCATCAGGAA+AGG + chr6.3:19473960-19473979 MS.gene49827:CDS 45.0%
GGTTGTGAAAATGGGAAGGT+GGG + chr6.3:19471885-19471904 MS.gene49827:CDS 45.0%
GTAAGGGAACATCTTCCATC+AGG + chr6.3:19473955-19473974 MS.gene49827:CDS 45.0%
GTCTCACAAGTGCTTGTGTT+AGG - chr6.3:19473461-19473480 None:intergenic 45.0%
GTTCAATCCAGGTCCATCTA+GGG + chr6.3:19472807-19472826 MS.gene49827:intron 45.0%
TCATCATCGTCAACCGTTCT+AGG - chr6.3:19471939-19471958 None:intergenic 45.0%
TCGAAGCAAAAAAGCAGAGG+CGG - chr6.3:19474107-19474126 None:intergenic 45.0%
TGGTTGTGAAAATGGGAAGG+TGG + chr6.3:19471884-19471903 MS.gene49827:CDS 45.0%
TTCAATCCAGGTCCATCTAG+GGG + chr6.3:19472808-19472827 MS.gene49827:intron 45.0%
TTCACCAATTCCGCCTAGAA+CGG + chr6.3:19471923-19471942 MS.gene49827:CDS 45.0%
TTGCTGCTACTCGATTTCCT+GGG - chr6.3:19471174-19471193 None:intergenic 45.0%
TTTGCAGGTTAGGGAGATGT+TGG - chr6.3:19471298-19471317 None:intergenic 45.0%
! AAGCTGTTTCGGAGAGCTTA+TGG - chr6.3:19473534-19473553 None:intergenic 45.0%
! ATGGGGTTTGTTTGCGAATC+AGG + chr6.3:19472154-19472173 MS.gene49827:CDS 45.0%
! ATTGGAAGAGAGTGTGGGTT+GGG + chr6.3:19472041-19472060 MS.gene49827:CDS 45.0%
! GATGATGAGTCGGTGAGTTT+TGG + chr6.3:19471951-19471970 MS.gene49827:CDS 45.0%
! GATGGTATTGGAAGAGAGTG+TGG + chr6.3:19472035-19472054 MS.gene49827:CDS 45.0%
! GGAAGGTGTGTAGAGAAAGT+AGG - chr6.3:19471436-19471455 None:intergenic 45.0%
! GGGTGTTGTGAGAGAGAATA+AGG - chr6.3:19471458-19471477 None:intergenic 45.0%
! GTAAGGAGGGTTGTTGTTGA+TGG - chr6.3:19471412-19471431 None:intergenic 45.0%
! GTTGGGTTGTTTATGTGGGA+GGG + chr6.3:19472058-19472077 MS.gene49827:CDS 45.0%
! TATTGGAAGAGAGTGTGGGT+TGG + chr6.3:19472040-19472059 MS.gene49827:CDS 45.0%
! TCAATAGGTGGTGGTGTTAG+TGG - chr6.3:19471624-19471643 None:intergenic 45.0%
!! AAGGTGTCAGTGATGATGGT+GGG + chr6.3:19471679-19471698 MS.gene49827:CDS 45.0%
!! ACCCCATGGTTGATAATTGG+GGG - chr6.3:19471478-19471497 None:intergenic 45.0%
!! AGCACCCCATGGTTGATAAT+TGG - chr6.3:19471481-19471500 None:intergenic 45.0%
!! CACCACCTATTGATGATGCT+GGG + chr6.3:19471631-19471650 MS.gene49827:CDS 45.0%
!! CACCCCATGGTTGATAATTG+GGG - chr6.3:19471479-19471498 None:intergenic 45.0%
!! GCACCCCATGGTTGATAATT+GGG - chr6.3:19471480-19471499 None:intergenic 45.0%
!! GGATCAGTGAGATTTGGTGT+TGG + chr6.3:19471864-19471883 MS.gene49827:CDS 45.0%
!! GTATGTGATGATGGGTCTGT+GGG + chr6.3:19471783-19471802 MS.gene49827:CDS 45.0%
!! GTGGGTTGGGTTGTTTATGT+GGG + chr6.3:19472054-19472073 MS.gene49827:CDS 45.0%
!! TGTGGGTTGGGTTGTTTATG+TGG + chr6.3:19472053-19472072 MS.gene49827:CDS 45.0%
!!! CAATTTTATATTTAATTTTT+AGG + chr6.3:19472649-19472668 MS.gene49827:intron 5.0%
AAGCAAAAAAGCAGAGGCGG+GGG - chr6.3:19474104-19474123 None:intergenic 50.0%
AGATGTTGGCGAGTTGTGTG+TGG - chr6.3:19471284-19471303 None:intergenic 50.0%
CCAGCATCATCAATAGGTGG+TGG - chr6.3:19471633-19471652 None:intergenic 50.0%
CCTAGAGCGATCGATGATGA+GGG + chr6.3:19471843-19471862 MS.gene49827:CDS 50.0%
CGAAGCAAAAAAGCAGAGGC+GGG - chr6.3:19474106-19474125 None:intergenic 50.0%
GAAAATGGGTCAGTGGGAGA+TGG + chr6.3:19471576-19471595 MS.gene49827:CDS 50.0%
GAAAGGCTGTTGCAGTGACA+CGG - chr6.3:19474198-19474217 None:intergenic 50.0%
GAAGCAAAAAAGCAGAGGCG+GGG - chr6.3:19474105-19474124 None:intergenic 50.0%
GATGTTGGCGAGTTGTGTGT+GGG - chr6.3:19471283-19471302 None:intergenic 50.0%
GGTGATCGAATTTACCGGAC+TGG - chr6.3:19472837-19472856 None:intergenic 50.0%
GTCCCAGCATCATCAATAGG+TGG - chr6.3:19471636-19471655 None:intergenic 50.0%
TATGATGAACTGGCATCGCG+TGG + chr6.3:19473907-19473926 MS.gene49827:CDS 50.0%
TCAGGAAAAGGTCCAATGGG+CGG + chr6.3:19474172-19474191 MS.gene49827:CDS 50.0%
TGCAACAGCCTTTCTTGCCT+TGG + chr6.3:19474204-19474223 MS.gene49827:CDS 50.0%
TGTGAAAATGGGAAGGTGGG+AGG + chr6.3:19471888-19471907 MS.gene49827:CDS 50.0%
TGTTTATGTGGGAGGGAGTG+TGG + chr6.3:19472065-19472084 MS.gene49827:CDS 50.0%
! CTTCTGAGGGCAATGTTCCA+AGG - chr6.3:19474224-19474243 None:intergenic 50.0%
! GATGCTGGGACAGTGAGTTT+TGG + chr6.3:19471645-19471664 MS.gene49827:CDS 50.0%
! GGTTGGGTTGTTTATGTGGG+AGG + chr6.3:19472057-19472076 MS.gene49827:CDS 50.0%
! TCAACCGTTCTAGGCGGAAT+TGG - chr6.3:19471930-19471949 None:intergenic 50.0%
! TCATCATCGATCGCTCTAGG+CGG - chr6.3:19471843-19471862 None:intergenic 50.0%
! TCATCGTCAACCGTTCTAGG+CGG - chr6.3:19471936-19471955 None:intergenic 50.0%
! TGATGACGATACCCCTGAGA+TGG + chr6.3:19473386-19473405 MS.gene49827:intron 50.0%
! TGGGAGGGAGTGTGGTAATA+GGG + chr6.3:19472073-19472092 MS.gene49827:CDS 50.0%
!! AGGTGCTCTAAGAAATCGGG+AGG + chr6.3:19473164-19473183 MS.gene49827:intron 50.0%
!! AGGTGTCAGTGATGATGGTG+GGG + chr6.3:19471680-19471699 MS.gene49827:CDS 50.0%
!! ATTCCGCCGGTTGATGAAGT+TGG + chr6.3:19471723-19471742 MS.gene49827:CDS 50.0%
!! CCACCACCTATTGATGATGC+TGG + chr6.3:19471630-19471649 MS.gene49827:CDS 50.0%
!! CGTATGTGATGATGGGTCTG+TGG + chr6.3:19471782-19471801 MS.gene49827:CDS 50.0%
!! GAAGGTGTCAGTGATGATGG+TGG + chr6.3:19471678-19471697 MS.gene49827:CDS 50.0%
!! GAGTCGGTGAGTTTTGGTGT+GGG + chr6.3:19471957-19471976 MS.gene49827:CDS 50.0%
!! GATGATGGGTCGGTGAGATT+TGG + chr6.3:19471525-19471544 MS.gene49827:CDS 50.0%
!! TGAGTCGGTGAGTTTTGGTG+TGG + chr6.3:19471956-19471975 MS.gene49827:CDS 50.0%
!! TGGGAAGGTGTCAGTGATGA+TGG + chr6.3:19471675-19471694 MS.gene49827:CDS 50.0%
!! TTCCGCCGGTTGATGAAGTT+GGG + chr6.3:19471724-19471743 MS.gene49827:CDS 50.0%
AATGGGTCAGTGGGAGATGG+TGG + chr6.3:19471579-19471598 MS.gene49827:CDS 55.0%
ACCGGCCCAACTTCATCAAC+CGG - chr6.3:19471732-19471751 None:intergenic 55.0%
ATGGGTCAGTGGGAGATGGT+GGG + chr6.3:19471580-19471599 MS.gene49827:CDS 55.0%
ATGGGTCTGTGGGAGATGGT+GGG + chr6.3:19471793-19471812 MS.gene49827:CDS 55.0%
CCCTCATCATCGATCGCTCT+AGG - chr6.3:19471846-19471865 None:intergenic 55.0%
CTCAAAGGTTCGCGAAGCGT+CGG - chr6.3:19471364-19471383 None:intergenic 55.0%
GAAAATGGGAAGGTGGGAGG+TGG + chr6.3:19471891-19471910 MS.gene49827:CDS 55.0%
GAAGTTGGGCCGGTGAAGTT+TGG + chr6.3:19471738-19471757 MS.gene49827:CDS 55.0%
GCCTAGAGCGATCGATGATG+AGG + chr6.3:19471842-19471861 MS.gene49827:CDS 55.0%
GCGACGGAGGGTGATGATTT+GGG - chr6.3:19471337-19471356 None:intergenic 55.0%
GGTGTGGGATGTGAGTGTGA+AGG + chr6.3:19471972-19471991 MS.gene49827:CDS 55.0%
TGCGACGGAGGGTGATGATT+TGG - chr6.3:19471338-19471357 None:intergenic 55.0%
TTACCTTCTCCAGCACCCCA+TGG - chr6.3:19471492-19471511 None:intergenic 55.0%
TTGTCTCCTCTGTGCTTCCG+TGG - chr6.3:19473881-19473900 None:intergenic 55.0%
! GTGGGAGGGAGTGTGGTAAT+AGG + chr6.3:19472072-19472091 MS.gene49827:CDS 55.0%
! TCGATCGCTCTAGGCGGAAT+TGG - chr6.3:19471837-19471856 None:intergenic 55.0%
! TGACAACCACGGAAGCACAG+AGG + chr6.3:19473872-19473891 MS.gene49827:CDS 55.0%
!! GATGATGGGTCTGTGGGAGA+TGG + chr6.3:19471789-19471808 MS.gene49827:CDS 55.0%
!! GGGTCGGTGAGATTTGGTGT+TGG + chr6.3:19471531-19471550 MS.gene49827:CDS 55.0%
!! GGTGTCAGTGATGATGGTGG+GGG + chr6.3:19471681-19471700 MS.gene49827:CDS 55.0%
!! GTATGGTGTGGTGGTGGGAA+AGG + chr6.3:19472131-19472150 MS.gene49827:CDS 55.0%
!! GTGTGGTGGTGGGAAAGGAT+GGG + chr6.3:19472136-19472155 MS.gene49827:CDS 55.0%
!! TGTGGTGGTGGGAAAGGATG+GGG + chr6.3:19472137-19472156 MS.gene49827:CDS 55.0%
CCATCTAGGGGAAGCCAGTC+CGG + chr6.3:19472820-19472839 MS.gene49827:intron 60.0%
GATGGGTCTGTGGGAGATGG+TGG + chr6.3:19471792-19471811 MS.gene49827:CDS 60.0%
GGCCCAACTTCATCAACCGG+CGG - chr6.3:19471729-19471748 None:intergenic 60.0%
TGGCTTCCCCTAGATGGACC+TGG - chr6.3:19472817-19472836 None:intergenic 60.0%
TGGGTCAGTGGGAGATGGTG+GGG + chr6.3:19471581-19471600 MS.gene49827:CDS 60.0%
TGGGTCTGTGGGAGATGGTG+GGG + chr6.3:19471794-19471813 MS.gene49827:CDS 60.0%
!! CAACCATGGGGTGCTGGAGA+AGG + chr6.3:19471486-19471505 MS.gene49827:CDS 60.0%
!! GCCGGTTGATGAAGTTGGGC+CGG + chr6.3:19471728-19471747 MS.gene49827:CDS 60.0%
!! GGGCCGGTGAAGTTTGGTGT+TGG + chr6.3:19471744-19471763 MS.gene49827:CDS 60.0%
!! GGTGTGGTGGTGGGAAAGGA+TGG + chr6.3:19472135-19472154 MS.gene49827:CDS 60.0%
! AGCGTCGGAGATGCGACGGA+GGG - chr6.3:19471349-19471368 None:intergenic 65.0%
! GGGTCAGTGGGAGATGGTGG+GGG + chr6.3:19471582-19471601 MS.gene49827:CDS 65.0%
! GGGTCTGTGGGAGATGGTGG+GGG + chr6.3:19471795-19471814 MS.gene49827:CDS 65.0%
!! AAGCGTCGGAGATGCGACGG+AGG - chr6.3:19471350-19471369 None:intergenic 65.0%
!! CCGGACTGGCTTCCCCTAGA+TGG - chr6.3:19472823-19472842 None:intergenic 65.0%
!! GCGAAGCGTCGGAGATGCGA+CGG - chr6.3:19471353-19471372 None:intergenic 65.0%
GCAGTGACACGGCCGCCCAT+TGG - chr6.3:19474187-19474206 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 19471162 19474251 19471162 ID=MS.gene49827
chr6.3 mRNA 19471162 19474251 19471162 ID=MS.gene49827.t1;Parent=MS.gene49827
chr6.3 exon 19471162 19472212 19471162 ID=MS.gene49827.t1.exon1;Parent=MS.gene49827.t1
chr6.3 CDS 19471162 19472212 19471162 ID=cds.MS.gene49827.t1;Parent=MS.gene49827.t1
chr6.3 exon 19473863 19474251 19473863 ID=MS.gene49827.t1.exon2;Parent=MS.gene49827.t1
chr6.3 CDS 19473863 19474251 19473863 ID=cds.MS.gene49827.t1;Parent=MS.gene49827.t1
Gene Sequence

>MS.gene49827

ATGAAGATACCCAGGAAATCGAGTAGCAGCAATGGTGATTCAAACTCTTCATCATCATCATCATCATCATCATCATCGTTTCTTGTTCAACACGCAACCCTAATTCTACCATATCTAACCCACACACAACTCGCCAACATCTCCCTAACCTGCAAATCACTCCATAAACTCACCCAAATCATCACCCTCCGTCGCATCTCCGACGCTTCGCGAACCTTTGAGAATCATCCAATCCCATTTCTCAACACCATCAACAACAACCCTCCTTACTCCTACTTTCTCTACACACCTTCCCTTATTCTCTCTCACAACACCCCCAATTATCAACCATGGGGTGCTGGAGAAGGTAATTCTGTCATTTCAGATGATGGGTCGGTGAGATTTGGTGTTGGTTGTGATTTTGAAGGTGTTTGTGAAAATGGGTCAGTGGGAGATGGTGGGGGTAGTTCTGTCATTTCACCACTAACACCACCACCTATTGATGATGCTGGGACAGTGAGTTTTGGTGTTGAATGGGAAGGTGTCAGTGATGATGGTGGGGGTAAATCAGTAATTTCACTAATTCCGCCGGTTGATGAAGTTGGGCCGGTGAAGTTTGGTGTTGGATGTAATTTTCAAGGCGTATGTGATGATGGGTCTGTGGGAGATGGTGGGGGTAGTTCAGTAATTTCACCAATTCCGCCTAGAGCGATCGATGATGAGGGATCAGTGAGATTTGGTGTTGGTTGTGAAAATGGGAAGGTGGGAGGTGGTTCAGTAATTTCACCAATTCCGCCTAGAACGGTTGACGATGATGAGTCGGTGAGTTTTGGTGTGGGATGTGAGTGTGAAGGTGTTTGTGATGTTGAGTGTATGTGTTTTTTTGATGGGGTTGATGGTATTGGAAGAGAGTGTGGGTTGGGTTGTTTATGTGGGAGGGAGTGTGGTAATAGGGTGAGTCAAAATGGGGTTAATGTTAGGGTTAAAATTGTATGGTGTGGTGGTGGGAAAGGATGGGGTTTGTTTGCGAATCAGGTTGTTCGTAAAGAGGAGTTCTTGTTTCAATATGCAGGTATAAGTTTCATTGCTTTTATTAAGTATTTAAGTGATTGTTTGATTGATTATAGGATTGTAACATTGGTTCTTATGTATATTTTAGTGTTTTTGCGTAGGATTGATTTGAATTTGAACGTGGAATTCGGAGGTCTTTTTGTCTCTATAATATATGTTAGAATTGATTCTAGAATCGTATAGTTGATTATGACGTGTTTTTGTGTTTTAGAGTTGGAATGATTTTTCCATTAGAAATTTAGAATTGATCCGAATTTGGTGCTAAAATTTTGAGATTTTGCGTTCATGATAGAAGCTAGAATTGATGCTAGAAATCATAAAGTGCTTGTTTGGATATATGTTGGTTTGACGTTTCTCGCGTCACAATGTGTTCTCAAGCTTAAGATTTTGAATTACATTTATGTTATTTGTATCAGTATATGTATGTGCGCGCTTTTCAATTTTATATTTAATTTTTAGGTATGGAATTCTTAATCTCAGCATAAACAAAGTCACGAACTAGAAAACACGAACAAACTCATTGTTTCAAATTGCAAAGAACATAACAGACCCAAAAGGTGAATAATTCTGATGAGTTAAAAACTAGCTCTACCAAGTTCAATCCAGGTCCATCTAGGGGAAGCCAGTCCGGTAAATTCGATCACCAAGTATTTTCTGGTTTTTCATACGGAATTGAACCTTGTTGCCAACAACTTGTTGGTATGATTCATCAATTTTACTTCTTCTACATGCTAGCTACATGCTAGAATGCGATTAATCCACATTCTCAAGTAGAACAAAACCACTGTACATTTTCAATTGCCATTTTGACAACCTTCCCTTGAGTGAAATATTAAGTTTTCATTGCAAGCTGAGCTGTATTTTACCCTAATTATTAGCTTGTTAAGATGATGGTCTATAGGTCAAGGGTGAAGTCTTGAATTTGATATTTCTATGTAAAAAGTTACTTTATAGGTGCTCTAAGAAATCGGGAGGTTTTATACTATAGTAGGAACACGGGTCTTTGGTTTTAGCAACCCACTTAGAAGGCAAAATCAATTTGCAAATTGCAATATGTTGAGTAGGAATGTTTCAAATCCGTAGATGTAAACTGCCTGTACTTTTGTATTTAAGTTAATAGTTAAGGTACTTTTGATATTTTGACTAATATAGAGATTTGAAAGGCTTTTTATTTTGATGACGATACCCCTGAGATGGTGACATTATCAAATATGAGTCTATTTGGATGACTTATTTGAGCTTATCTCCTAACACAAGCACTTGTGAGACTGTTTGGGAAACAACTTATGACGGGTCCATAAGCTGTTTTCAACGTATTTCCATAAGCTCTCCGAAACAGCTTATGAAAACAAATTGTAGCTTATATGAAAACAATTCGACTCTATGTTATCTTTTGTTATACAAATAGCTTATATATAAGCGCTGATGTTATAAGCGGTTAATTAAATTGTTTATCTAAACAGAGCCATATATGCTTATGAACTAAAGAGTTGTGTGAAGATTGATAGAAACTACCTCCTAGTGTTTGCTATGTTAAGGTGATTGGGTCTGATAGTTTCTTGATTCTTTTAGTTGTGTTTTTATGCATGACATTTCTTTATCCCTACACAAATGCTTGTAAAATTAATTTGCGGTTTTCTGTGTGAAAAAGGTGAACTACTGACAACCACGGAAGCACAGAGGAGACAACAACATTATGATGAACTGGCATCGCGTGGTCGTTTCTCGTCTGCTCTTTTAGTAGTAAGGGAACATCTTCCATCAGGAAAGGCATGTTTGAGATTGAACATAGACGCAACAAAAATAGGAAATGTTGCCCGGTTTGTAAATCATTCATGCGATGGTGGTAATTTAAGCACAAAGCTAGTCAGAAGTACAAGAGCTTTGTTCCCCCGCCTCTGCTTTTTTGCTTCGAAAGATATCCAAAAAGATGAAGAGCTTGCTTTTAGCTATGGAGAAATCAGGAAAAGGTCCAATGGGCGGCCGTGTCACTGCAACAGCCTTTCTTGCCTTGGAACATTGCCCTCAGAAGACACTTGA
Protein sequence

>MS.gene49827.t1

MKIPRKSSSSNGDSNSSSSSSSSSSSFLVQHATLILPYLTHTQLANISLTCKSLHKLTQIITLRRISDASRTFENHPIPFLNTINNNPPYSYFLYTPSLILSHNTPNYQPWGAGEGNSVISDDGSVRFGVGCDFEGVCENGSVGDGGGSSVISPLTPPPIDDAGTVSFGVEWEGVSDDGGGKSVISLIPPVDEVGPVKFGVGCNFQGVCDDGSVGDGGGSSVISPIPPRAIDDEGSVRFGVGCENGKVGGGSVISPIPPRTVDDDESVSFGVGCECEGVCDVECMCFFDGVDGIGRECGLGCLCGRECGNRVSQNGVNVRVKIVWCGGGKGWGLFANQVVRKEEFLFQYAGELLTTTEAQRRQQHYDELASRGRFSSALLVVREHLPSGKACLRLNIDATKIGNVARFVNHSCDGGNLSTKLVRSTRALFPRLCFFASKDIQKDEELAFSYGEIRKRSNGRPCHCNSLSCLGTLPSEDT