Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49855.t1 | XP_003597050.2 | 96 | 2497 | 76 | 2 | 1 | 2475 | 1 | 2496 | 0.00E+00 | 4372 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49855.t1 | O23372 | 51.6 | 2332 | 842 | 31 | 183 | 2474 | 249 | 2334 | 0.0e+00 | 2113.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49855.t1 | G7IRJ2 | 96.0 | 2497 | 76 | 2 | 1 | 2475 | 1 | 2496 | 0.0e+00 | 4372.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene49855.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49855.t1 | MTR_2g089080 | 96.996 | 2497 | 52 | 2 | 1 | 2475 | 1 | 2496 | 0.0 | 5026 |
| MS.gene49855.t1 | MTR_4g045743 | 63.429 | 2677 | 758 | 39 | 1 | 2475 | 1 | 2658 | 0.0 | 3159 |
| MS.gene49855.t1 | MTR_5g037343 | 44.765 | 277 | 63 | 5 | 1673 | 1949 | 229 | 415 | 1.07e-50 | 192 |
| MS.gene49855.t1 | MTR_5g037343 | 50.641 | 156 | 43 | 2 | 1237 | 1391 | 60 | 182 | 1.53e-33 | 140 |
| MS.gene49855.t1 | MTR_5g037343 | 62.069 | 58 | 10 | 1 | 1521 | 1578 | 176 | 221 | 7.81e-12 | 71.2 |
| MS.gene49855.t1 | MTR_3g088625 | 30.323 | 155 | 85 | 5 | 1919 | 2071 | 1329 | 1462 | 2.69e-12 | 73.6 |
| MS.gene49855.t1 | MTR_3g088625 | 30.323 | 155 | 85 | 5 | 1919 | 2071 | 1223 | 1356 | 2.76e-12 | 73.6 |
| MS.gene49855.t1 | MTR_2g036060 | 36.567 | 134 | 66 | 5 | 1934 | 2067 | 1075 | 1189 | 9.80e-11 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49855.t1 | AT4G15180 | 56.389 | 2027 | 721 | 27 | 495 | 2474 | 424 | 2334 | 0.0 | 2144 |
| MS.gene49855.t1 | AT1G77300 | 30.928 | 194 | 101 | 9 | 1891 | 2071 | 991 | 1164 | 1.82e-12 | 73.9 |
| MS.gene49855.t1 | AT1G77300 | 30.412 | 194 | 102 | 8 | 1891 | 2071 | 991 | 1164 | 1.86e-12 | 73.9 |
| MS.gene49855.t1 | AT3G59960 | 30.065 | 153 | 72 | 5 | 1925 | 2070 | 122 | 246 | 1.34e-11 | 68.6 |
| MS.gene49855.t1 | AT3G59960 | 30.065 | 153 | 72 | 5 | 1925 | 2070 | 122 | 246 | 1.34e-11 | 68.6 |
| MS.gene49855.t1 | AT3G59960 | 30.065 | 153 | 72 | 5 | 1925 | 2070 | 122 | 246 | 1.34e-11 | 68.6 |
| MS.gene49855.t1 | AT3G59960 | 30.065 | 153 | 72 | 5 | 1925 | 2070 | 122 | 246 | 3.08e-11 | 67.8 |
| MS.gene49855.t1 | AT5G42400 | 35.211 | 142 | 67 | 5 | 1934 | 2072 | 1267 | 1386 | 5.96e-11 | 68.9 |
| MS.gene49855.t1 | AT5G42400 | 35.211 | 142 | 67 | 5 | 1934 | 2072 | 1267 | 1386 | 5.96e-11 | 68.9 |
| MS.gene49855.t1 | AT5G42400 | 35.211 | 142 | 67 | 5 | 1934 | 2072 | 1267 | 1386 | 5.96e-11 | 68.9 |
| MS.gene49855.t1 | AT5G42400 | 35.211 | 142 | 67 | 5 | 1934 | 2072 | 1267 | 1386 | 5.96e-11 | 68.9 |
| MS.gene49855.t1 | AT5G42400 | 35.211 | 142 | 67 | 5 | 1934 | 2072 | 1267 | 1386 | 5.96e-11 | 68.9 |
| MS.gene49855.t1 | AT5G42400 | 35.211 | 142 | 67 | 5 | 1934 | 2072 | 1267 | 1386 | 5.96e-11 | 68.9 |
| MS.gene49855.t1 | AT5G42400 | 35.211 | 142 | 67 | 5 | 1934 | 2072 | 1267 | 1386 | 5.96e-11 | 68.9 |
| MS.gene49855.t1 | AT5G42400 | 35.211 | 142 | 67 | 5 | 1934 | 2072 | 1267 | 1386 | 5.96e-11 | 68.9 |
| MS.gene49855.t1 | AT2G44150 | 30.822 | 146 | 80 | 4 | 1925 | 2070 | 127 | 251 | 6.32e-11 | 67.4 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15130 | gene | 43911 | 57085 | 43911 | ID=MS.gene49855 |
| 15130 | mRNA | 43911 | 57085 | 43911 | ID=MS.gene49855.t1;Parent=MS.gene49855 |
| 15130 | exon | 43911 | 46413 | 43911 | ID=MS.gene49855.t1.exon1;Parent=MS.gene49855.t1 |
| 15130 | CDS | 43911 | 46413 | 43911 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 46547 | 46603 | 46547 | ID=MS.gene49855.t1.exon2;Parent=MS.gene49855.t1 |
| 15130 | CDS | 46547 | 46603 | 46547 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 47392 | 48543 | 47392 | ID=MS.gene49855.t1.exon3;Parent=MS.gene49855.t1 |
| 15130 | CDS | 47392 | 48543 | 47392 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 48768 | 49138 | 48768 | ID=MS.gene49855.t1.exon4;Parent=MS.gene49855.t1 |
| 15130 | CDS | 48768 | 49138 | 48768 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 49266 | 49814 | 49266 | ID=MS.gene49855.t1.exon5;Parent=MS.gene49855.t1 |
| 15130 | CDS | 49266 | 49814 | 49266 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 49902 | 50002 | 49902 | ID=MS.gene49855.t1.exon6;Parent=MS.gene49855.t1 |
| 15130 | CDS | 49902 | 50002 | 49902 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 50521 | 51559 | 50521 | ID=MS.gene49855.t1.exon7;Parent=MS.gene49855.t1 |
| 15130 | CDS | 50521 | 51559 | 50521 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 51661 | 51732 | 51661 | ID=MS.gene49855.t1.exon8;Parent=MS.gene49855.t1 |
| 15130 | CDS | 51661 | 51732 | 51661 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 52395 | 52505 | 52395 | ID=MS.gene49855.t1.exon9;Parent=MS.gene49855.t1 |
| 15130 | CDS | 52395 | 52505 | 52395 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 52605 | 52705 | 52605 | ID=MS.gene49855.t1.exon10;Parent=MS.gene49855.t1 |
| 15130 | CDS | 52605 | 52705 | 52605 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 54149 | 54248 | 54149 | ID=MS.gene49855.t1.exon11;Parent=MS.gene49855.t1 |
| 15130 | CDS | 54149 | 54248 | 54149 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 54352 | 54447 | 54352 | ID=MS.gene49855.t1.exon12;Parent=MS.gene49855.t1 |
| 15130 | CDS | 54352 | 54447 | 54352 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 54529 | 54696 | 54529 | ID=MS.gene49855.t1.exon13;Parent=MS.gene49855.t1 |
| 15130 | CDS | 54529 | 54696 | 54529 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 54786 | 54911 | 54786 | ID=MS.gene49855.t1.exon14;Parent=MS.gene49855.t1 |
| 15130 | CDS | 54786 | 54911 | 54786 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 55261 | 55314 | 55261 | ID=MS.gene49855.t1.exon15;Parent=MS.gene49855.t1 |
| 15130 | CDS | 55261 | 55314 | 55261 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 55698 | 55945 | 55698 | ID=MS.gene49855.t1.exon16;Parent=MS.gene49855.t1 |
| 15130 | CDS | 55698 | 55945 | 55698 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 56037 | 56145 | 56037 | ID=MS.gene49855.t1.exon17;Parent=MS.gene49855.t1 |
| 15130 | CDS | 56037 | 56145 | 56037 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 56243 | 56545 | 56243 | ID=MS.gene49855.t1.exon18;Parent=MS.gene49855.t1 |
| 15130 | CDS | 56243 | 56545 | 56243 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| 15130 | exon | 56918 | 57085 | 56918 | ID=MS.gene49855.t1.exon19;Parent=MS.gene49855.t1 |
| 15130 | CDS | 56918 | 57085 | 56918 | ID=cds.MS.gene49855.t1;Parent=MS.gene49855.t1 |
| Gene Sequence |
| Protein sequence |
