AlfalfaGEDB | MS.gene50014

M. sativa cultivar XinJiangDaYe / MS.gene50014

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50014.t1	AFK42063.1	93	186	10	3	1	183	1	186	2.80E-95	358.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50014.t1	Q9FY93	73.8	160	40	1	1	158	1	160	4.5e-70	265.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50014.t1	G7KVL9	93.0	186	10	3	1	183	1	186	2.0e-95	358.2

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene50014.t1	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50014.t1	MTR_7g005280	80.078	256	20	6	1	225	1	256	1.50e-134	378
MS.gene50014.t1	MTR_8g099750	60.227	176	59	3	1	166	1	175	7.34e-74	223
MS.gene50014.t1	MTR_3g098810	59.551	178	60	3	1	166	1	178	6.54e-71	216
MS.gene50014.t1	MTR_3g098810	62.252	151	54	2	1	148	1	151	4.72e-64	197
MS.gene50014.t1	MTR_4g089135	54.857	175	50	3	13	158	14	188	6.87e-58	186
MS.gene50014.t1	MTR_7g085220	45.946	222	91	7	11	203	5	226	1.48e-57	186
MS.gene50014.t1	MTR_5g041940	56.962	158	56	3	12	159	1	156	7.59e-57	181
MS.gene50014.t1	MTR_4g081870	53.977	176	69	4	14	179	9	182	8.68e-57	181
MS.gene50014.t1	MTR_2g080010	56.790	162	53	3	14	158	15	176	1.22e-56	184
MS.gene50014.t1	MTR_7g100990	47.368	190	89	4	9	188	6	194	3.05e-55	179

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50014.t1	AT5G13180	73.054	167	43	1	1	165	1	167	8.77e-92	270
MS.gene50014.t1	AT2G33480	60.488	205	77	3	1	203	1	203	1.74e-85	254
MS.gene50014.t1	AT2G33480	59.903	207	77	3	1	203	1	205	3.78e-85	254
MS.gene50014.t1	AT1G61110	56.886	167	53	4	10	158	12	177	1.09e-56	183
MS.gene50014.t1	AT1G77450	49.746	197	85	4	10	196	6	198	1.85e-56	180
MS.gene50014.t1	AT3G04070	55.689	167	58	4	8	158	4	170	3.81e-56	182
MS.gene50014.t1	AT1G69490	57.143	161	56	4	14	164	9	166	3.88e-56	180
MS.gene50014.t1	AT5G39610	45.714	210	99	5	12	208	18	225	5.08e-55	177
MS.gene50014.t1	AT1G65910	45.933	209	91	6	12	210	4	200	4.36e-54	183
MS.gene50014.t1	AT3G04070	52.542	177	52	4	14	158	10	186	5.06e-54	177

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Find 50 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGGATGGGATTGAGG+AGG	0.660083	6.2:+77067671	None:intergenic
AAAGAAGCCAAGTATCCAAA+TGG	0.440359	6.2:-77068198	MS.gene50014:CDS
AAGAAGCCAAGTATCCAAAT+GGG	0.544681	6.2:-77068197	MS.gene50014:CDS
AAGATCAACTTCAGGAATGA+TGG	0.468508	6.2:+77068470	None:intergenic
AAGCATTTCCAAAAGAGGAT+GGG	0.533996	6.2:+77067661	None:intergenic
AATGGAGAGGAGACAGTGAA+AGG	0.524667	6.2:-77067822	MS.gene50014:CDS
ACAACAAGTTCTTCATCTGT+TGG	0.464543	6.2:+77068537	None:intergenic
ACTTCAGGAATGATGGAAGC+AGG	0.542259	6.2:+77068477	None:intergenic
AGAAAGACAGACCAAGGGGT+TGG	0.554284	6.2:+77067987	None:intergenic
AGAGGATGGGATTGAGGAGG+AGG	0.387672	6.2:+77067674	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAGTAGAAGTTATCATGTTC+CGG	+	chr6.2:77067954-77067973	None:intergenic	30.0%
AAAGAAGCCAAGTATCCAAA+TGG	-	chr6.2:77068061-77068080	MS.gene50014:CDS	35.0%
AAGAAGCCAAGTATCCAAAT+GGG	-	chr6.2:77068062-77068081	MS.gene50014:CDS	35.0%
AAGATCAACTTCAGGAATGA+TGG	+	chr6.2:77067792-77067811	None:intergenic	35.0%
AAGCATTTCCAAAAGAGGAT+GGG	+	chr6.2:77068601-77068620	None:intergenic	35.0%
ACAACAAGTTCTTCATCTGT+TGG	+	chr6.2:77067725-77067744	None:intergenic	35.0%
AGTTTGTAGCTCTCTTAGAT+CGG	+	chr6.2:77068090-77068109	None:intergenic	35.0%
ATTCAAAGAAAGACAGACCA+AGG	+	chr6.2:77068281-77068300	None:intergenic	35.0%
CTTCATCTGTTGGATGAAAA+CGG	+	chr6.2:77067715-77067734	None:intergenic	35.0%
TAAGCATTTCCAAAAGAGGA+TGG	+	chr6.2:77068602-77068621	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	77067659	77068622	77067659	ID=MS.gene50014
chr6.2	mRNA	77067659	77068622	77067659	ID=MS.gene50014.t1;Parent=MS.gene50014
chr6.2	exon	77068442	77068622	77068442	ID=MS.gene50014.t1.exon1;Parent=MS.gene50014.t1
chr6.2	CDS	77068442	77068622	77068442	ID=cds.MS.gene50014.t1;Parent=MS.gene50014.t1
chr6.2	exon	77067999	77068255	77067999	ID=MS.gene50014.t1.exon2;Parent=MS.gene50014.t1
chr6.2	CDS	77067999	77068255	77067999	ID=cds.MS.gene50014.t1;Parent=MS.gene50014.t1
chr6.2	exon	77067659	77067898	77067659	ID=MS.gene50014.t1.exon3;Parent=MS.gene50014.t1
chr6.2	CDS	77067659	77067898	77067659	ID=cds.MS.gene50014.t1;Parent=MS.gene50014.t1

Gene Sequence

>MS.gene50014

ATGGAGAAGATGAATTTTGTGAAGAATGGTGAAGTGAGATTGCCACCAGGTTTCCGTTTTCATCCAACAGATGAAGAACTTGTTGTTCAATACTTGAAGCGTAAGGTCTTCTCTTTCCCTTTACCTGCTTCCATCATTCCTGAAGTTGATCTTTGCAAGTCTGATCCTTGGGATTTACCTGGTATGTACCTTTTCTTTTCTTTTCTTTTCTTTTTCGTTCGTTTATAATCATAACTTAATGAAGCACAGACACAGACATCGGACACAACATGGACACTGACACATTCAGACACCGGAACATGATAACTTCTACTACTACTGCAAACAATGTCATGTGGATTGATTTGAGTGTGTGTTGTTCTTTTAGGTGATATGGAACAAGATAGGTACTTTTTCAGTACAAAAGAAGCCAAGTATCCAAATGGGAACCGATCTAAGAGAGCTACAAACTCTGGTTACTGGAAAGCAACTGGTTTGGACAAACAAATCATGAATTCAAAGACTCATGAAGTTGTTGGCATGAAGAAAACTCTTGTTTTCTACAGAGGCAAACCACCTCATGGTTCCAGAACTGATTGGATCATGCATGAATATCGCCTCACTTCCTCTCACTCCAACCCCTTGGTCTGTCTTTCTTTGAATATGTACATTTGTAATTAAGAGTTTAGTCTTGTAATTTAAAGGTGTTGAATTAATATTAATATGAATAAATTGGGTTTTGCAGAATGAAAATTGGGTTCTGTGTCGCATATTTCTGAAGAGGAGAGGTGGTGCTAAAAATGGAGAGGAGACAGTGAAAGGAGTGAAGCCAAGCAATGACAGCAGTTTTGGTGAGGTGAAGAAGAACTCAGCTTCAACTTCAAGTTCAAAAGTGGTTGTTTTCTATGATTTCTTGGCAGAGAAGAAGAAAAATTCTGATGCATCCTCCTCCTCCTCAATCCCATCCTCTTTTGGAAATGCTTAA

Protein sequence

>MS.gene50014.t1

MEKMNFVKNGEVRLPPGFRFHPTDEELVVQYLKRKVFSFPLPASIIPEVDLCKSDPWDLPGDMEQDRYFFSTKEAKYPNGNRSKRATNSGYWKATGLDKQIMNSKTHEVVGMKKTLVFYRGKPPHGSRTDWIMHEYRLTSSHSNPLNENWVLCRIFLKRRGGAKNGEETVKGVKPSNDSSFGEVKKNSASTSSSKVVVFYDFLAEKKKNSDASSSSSIPSSFGNA