AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50087

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50087.t1 MTR_2g100470 94.444 72 4 0 80 151 553 624 7.53e-41 145
MS.gene50087.t1 MTR_2g100450 88.889 72 8 0 80 151 370 441 8.65e-39 139
MS.gene50087.t1 MTR_2g100450 88.889 72 8 0 80 151 575 646 2.04e-38 138
MS.gene50087.t1 MTR_2g100450 88.889 72 8 0 80 151 575 646 2.49e-38 137
MS.gene50087.t1 MTR_8g015340 63.889 72 25 1 80 151 599 669 4.20e-22 91.7
MS.gene50087.t1 MTR_8g014730 54.167 72 33 0 80 151 566 637 1.51e-20 87.4
MS.gene50087.t1 MTR_8g014930 55.556 72 32 0 80 151 571 642 1.88e-20 87.0
MS.gene50087.t1 MTR_8g014970 42.718 103 55 1 53 151 301 403 1.99e-20 87.0
MS.gene50087.t1 MTR_8g014930 55.556 72 32 0 80 151 566 637 2.06e-20 87.0
MS.gene50087.t1 MTR_8g015040 55.556 72 32 0 80 151 618 689 5.24e-20 85.9
MS.gene50087.t1 MTR_8g461120 55.556 72 32 0 80 151 580 651 7.18e-20 85.5
MS.gene50087.t1 MTR_8g461120 55.556 72 32 0 80 151 556 627 8.01e-20 85.1
MS.gene50087.t1 MTR_8g461120 55.556 72 32 0 80 151 580 651 8.20e-20 85.1
MS.gene50087.t1 MTR_8g015150 52.778 72 34 0 80 151 561 632 2.24e-19 84.0
MS.gene50087.t1 MTR_8g015150 52.778 72 34 0 80 151 561 632 2.46e-19 84.0
MS.gene50087.t1 MTR_8g015150 41.748 103 56 1 53 151 285 387 2.52e-19 83.6
MS.gene50087.t1 MTR_8g015150 52.778 72 34 0 80 151 561 632 2.75e-19 83.6
MS.gene50087.t1 MTR_8g014760 42.857 98 52 1 58 151 538 635 4.37e-19 83.2
MS.gene50087.t1 MTR_8g014760 42.857 98 52 1 58 151 542 639 4.58e-19 83.2
MS.gene50087.t1 MTR_8g015100 52.778 72 34 0 80 151 264 335 4.72e-19 82.8
MS.gene50087.t1 MTR_8g015100 52.778 72 34 0 80 151 481 552 5.44e-19 82.8
MS.gene50087.t1 MTR_2g100550 58.228 79 17 3 73 151 340 402 5.65e-19 82.8
MS.gene50087.t1 MTR_6g090615 54.167 72 33 0 80 151 572 643 6.27e-19 82.8
MS.gene50087.t1 MTR_8g014790 54.167 72 33 0 80 151 569 640 6.75e-19 82.4
MS.gene50087.t1 MTR_8g014860 50.000 72 36 0 80 151 91 162 1.39e-18 79.0
MS.gene50087.t1 MTR_8g014700 52.778 72 34 0 80 151 572 643 1.81e-18 81.3
MS.gene50087.t1 MTR_8g015010 51.389 72 35 0 80 151 616 687 1.84e-18 81.3
MS.gene50087.t1 MTR_8g014700 52.778 72 34 0 80 151 572 643 2.05e-18 81.3
MS.gene50087.t1 MTR_8g015190 54.167 72 33 0 80 151 603 674 2.08e-18 81.3
MS.gene50087.t1 MTR_8g014700 52.778 72 34 0 80 151 572 643 2.12e-18 81.3
MS.gene50087.t1 MTR_8g015190 54.167 72 33 0 80 151 593 664 2.31e-18 81.3
MS.gene50087.t1 MTR_8g014930 56.522 69 30 0 83 151 535 603 2.43e-18 80.9
MS.gene50087.t1 MTR_8g014930 56.522 69 30 0 83 151 535 603 2.70e-18 80.9
MS.gene50087.t1 MTR_8g014860 50.000 72 36 0 80 151 91 162 4.36e-18 78.6
MS.gene50087.t1 MTR_8g015200 51.389 72 35 0 80 151 543 614 6.46e-18 79.7
MS.gene50087.t1 MTR_8g028110 50.000 72 36 0 80 151 573 644 7.35e-18 79.7
MS.gene50087.t1 MTR_8g014690 50.000 72 36 0 80 151 577 648 2.05e-17 78.2
MS.gene50087.t1 MTR_8g014690 50.000 72 36 0 80 151 570 641 2.32e-17 78.2
MS.gene50087.t1 MTR_7g015240 42.857 70 40 0 82 151 525 594 1.41e-12 64.3
MS.gene50087.t1 MTR_7g061220 41.667 72 42 0 80 151 99 170 3.24e-12 63.2
MS.gene50087.t1 MTR_7g100630 41.667 72 42 0 80 151 605 676 3.69e-12 63.2
MS.gene50087.t1 MTR_6g088510 43.750 64 36 0 87 150 106 169 5.39e-12 62.8
MS.gene50087.t1 MTR_3g086120 41.538 65 38 0 80 144 609 673 7.46e-12 62.4
MS.gene50087.t1 MTR_3g086120 41.538 65 38 0 80 144 609 673 8.42e-12 62.0
MS.gene50087.t1 MTR_5g058090 43.056 72 41 0 80 151 586 657 8.66e-12 62.0
MS.gene50087.t1 MTR_7g015390 40.541 74 42 1 80 151 532 605 1.18e-11 61.6
MS.gene50087.t1 MTR_1g066950 43.056 72 41 0 80 151 599 670 1.24e-11 61.6
MS.gene50087.t1 MTR_8g064690 38.571 70 43 0 73 142 305 374 1.27e-11 61.6
MS.gene50087.t1 MTR_8g037700 29.508 122 74 1 42 151 482 603 1.65e-11 61.2
MS.gene50087.t1 MTR_7g015670 41.176 68 40 0 84 151 541 608 1.80e-11 61.2
MS.gene50087.t1 MTR_2g080220 30.693 101 58 1 63 151 474 574 2.41e-11 60.8
MS.gene50087.t1 MTR_7g015230 43.750 64 36 0 88 151 544 607 2.59e-11 60.8
MS.gene50087.t1 MTR_7g015510 41.892 74 41 1 80 151 532 605 3.46e-11 60.5
MS.gene50087.t1 MTR_7g015550 41.892 74 41 1 80 151 532 605 3.67e-11 60.5
MS.gene50087.t1 MTR_7g015250 42.647 68 39 0 84 151 524 591 4.37e-11 60.1
MS.gene50087.t1 MTR_5g030920 38.028 71 44 0 81 151 575 645 4.41e-11 60.1
MS.gene50087.t1 MTR_5g030920 38.028 71 44 0 81 151 598 668 4.46e-11 60.1
MS.gene50087.t1 MTR_6g088610 43.077 65 37 0 87 151 303 367 5.30e-11 59.7
MS.gene50087.t1 MTR_7g015310 40.541 74 42 1 80 151 524 597 6.58e-11 59.7
MS.gene50087.t1 MTR_7g015280 41.892 74 41 1 80 151 514 587 6.96e-11 59.7
MS.gene50087.t1 MTR_4g109010 34.831 89 58 0 63 151 521 609 7.02e-11 59.7
MS.gene50087.t1 MTR_4g095045 38.571 70 43 0 82 151 309 378 7.11e-11 59.3
MS.gene50087.t1 MTR_8g077850 38.571 70 43 0 82 151 340 409 9.15e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50087.t1 AT1G51890 65.278 72 24 1 80 151 299 369 2.89e-25 100
MS.gene50087.t1 AT1G51890 65.278 72 24 1 80 151 507 577 3.25e-25 100
MS.gene50087.t1 AT1G51890 65.278 72 24 1 80 151 571 641 3.75e-25 100
MS.gene50087.t1 AT1G51890 65.278 72 24 1 80 151 571 641 3.88e-25 100
MS.gene50087.t1 AT1G51890 65.278 72 24 1 80 151 571 641 3.93e-25 100
MS.gene50087.t1 AT1G51880 53.684 95 36 3 64 151 562 655 5.43e-24 97.1
MS.gene50087.t1 AT1G51880 63.889 72 25 1 80 151 593 663 5.93e-24 97.1
MS.gene50087.t1 AT1G51880 63.889 72 25 1 80 151 567 637 6.39e-24 97.1
MS.gene50087.t1 AT1G51860 63.889 72 25 1 80 151 441 511 8.46e-24 96.7
MS.gene50087.t1 AT1G51860 63.889 72 25 1 80 151 585 655 1.05e-23 96.3
MS.gene50087.t1 AT1G51870 62.500 72 26 1 80 151 584 654 1.22e-23 96.3
MS.gene50087.t1 AT1G51870 62.500 72 26 1 80 151 532 602 1.35e-23 96.3
MS.gene50087.t1 AT3G21340 59.722 72 29 0 80 151 593 664 2.36e-23 95.5
MS.gene50087.t1 AT3G21340 59.722 72 29 0 80 151 593 664 2.58e-23 95.1
MS.gene50087.t1 AT2G04300 58.333 72 30 0 80 151 583 654 5.33e-23 94.4
MS.gene50087.t1 AT2G04300 58.333 72 30 0 80 151 542 613 5.35e-23 94.4
MS.gene50087.t1 AT1G51820 56.944 72 31 0 80 151 317 388 9.54e-23 93.6
MS.gene50087.t1 AT1G51805 58.333 72 30 0 80 151 554 625 9.67e-23 93.6
MS.gene50087.t1 AT1G51805 58.333 72 30 0 80 151 578 649 9.99e-23 93.6
MS.gene50087.t1 AT1G51820 56.944 72 31 0 80 151 579 650 1.34e-22 93.2
MS.gene50087.t1 AT1G51850 56.944 72 31 0 80 151 579 650 1.44e-22 93.2
MS.gene50087.t1 AT1G51850 56.944 72 31 0 80 151 559 630 1.47e-22 93.2
MS.gene50087.t1 AT1G51830 56.944 72 31 0 80 151 387 458 3.00e-22 92.0
MS.gene50087.t1 AT1G51830 56.944 72 31 0 80 151 394 465 3.18e-22 92.0
MS.gene50087.t1 AT1G51810 55.556 72 32 0 80 151 451 522 1.51e-20 87.4
MS.gene50087.t1 AT1G51910 60.274 73 27 2 80 151 574 645 2.27e-20 87.0
MS.gene50087.t1 AT2G28970 52.778 72 34 0 80 151 445 516 4.72e-20 85.9
MS.gene50087.t1 AT2G19210 58.333 72 29 1 80 151 577 647 4.77e-20 85.9
MS.gene50087.t1 AT2G28970 52.778 72 34 0 80 151 480 551 5.14e-20 85.9
MS.gene50087.t1 AT4G29990 56.944 72 30 1 80 151 575 645 8.49e-20 85.1
MS.gene50087.t1 AT4G29990 56.944 72 30 1 80 151 575 645 9.03e-20 85.1
MS.gene50087.t1 AT5G16900 51.389 72 35 0 80 151 574 645 1.77e-19 84.3
MS.gene50087.t1 AT5G16900 51.389 72 35 0 80 151 574 645 1.77e-19 84.3
MS.gene50087.t1 AT5G16900 51.389 72 35 0 80 151 388 459 1.82e-19 84.3
MS.gene50087.t1 AT2G14510 54.167 72 32 1 80 151 572 642 5.45e-19 82.8
MS.gene50087.t1 AT2G14510 54.167 72 32 1 80 151 564 634 5.66e-19 82.8
MS.gene50087.t1 AT2G14440 54.167 72 32 1 80 151 565 635 1.03e-18 82.0
MS.gene50087.t1 AT2G28990 51.389 72 35 0 80 151 578 649 1.63e-18 81.6
MS.gene50087.t1 AT2G28990 51.389 72 35 0 80 151 578 649 1.63e-18 81.6
MS.gene50087.t1 AT2G28960 50.000 72 36 0 80 151 442 513 1.97e-18 81.3
MS.gene50087.t1 AT2G28960 50.000 72 36 0 80 151 574 645 2.07e-18 81.3
MS.gene50087.t1 AT3G46420 48.611 72 37 0 80 151 532 603 3.71e-18 80.5
MS.gene50087.t1 AT1G07560 52.113 71 34 0 81 151 572 642 3.87e-18 80.5
MS.gene50087.t1 AT4G20450 51.389 72 35 0 80 151 592 663 4.44e-18 80.1
MS.gene50087.t1 AT4G20450 51.389 72 35 0 80 151 592 663 4.76e-18 80.1
MS.gene50087.t1 AT2G29000 50.704 71 35 0 81 151 567 637 6.65e-18 79.7
MS.gene50087.t1 AT2G19230 54.167 72 32 1 80 151 570 640 9.90e-18 79.3
MS.gene50087.t1 AT2G19230 54.167 72 32 1 80 151 571 641 1.09e-17 79.3
MS.gene50087.t1 AT1G51800 51.389 72 34 1 80 151 587 657 1.81e-17 78.6
MS.gene50087.t1 AT2G19190 52.778 72 33 1 80 151 575 645 2.50e-17 78.2
MS.gene50087.t1 AT3G46400 50.704 71 35 0 81 151 578 648 3.13e-17 77.8
MS.gene50087.t1 AT1G49100 48.611 72 37 0 80 151 582 653 3.22e-17 77.8
MS.gene50087.t1 AT3G46370 50.000 72 36 0 80 151 488 559 6.81e-17 77.0
MS.gene50087.t1 AT3G46370 50.000 72 36 0 80 151 488 559 6.81e-17 77.0
MS.gene50087.t1 AT5G59670 50.000 72 36 0 80 151 565 636 1.04e-16 76.3
MS.gene50087.t1 AT5G59670 50.000 72 36 0 80 151 565 636 1.04e-16 76.3
MS.gene50087.t1 AT1G07550 48.611 72 36 1 80 151 561 631 3.99e-16 74.7
MS.gene50087.t1 AT3G46340 49.315 73 36 1 80 151 586 658 1.20e-15 73.2
MS.gene50087.t1 AT1G05700 50.000 72 35 1 80 151 602 672 2.58e-15 72.4
MS.gene50087.t1 AT1G05700 50.000 72 35 1 80 151 571 641 2.75e-15 72.4
MS.gene50087.t1 AT3G46330 45.833 72 39 0 80 151 567 638 2.76e-15 72.4
MS.gene50087.t1 AT3G46330 45.833 72 39 0 80 151 543 614 2.88e-15 72.4
MS.gene50087.t1 AT5G59680 54.386 57 26 0 95 151 596 652 1.46e-14 70.1
MS.gene50087.t1 AT4G29180 44.706 85 34 1 80 151 566 650 3.74e-14 68.9
MS.gene50087.t1 AT4G29180 44.706 85 34 1 80 151 565 649 3.85e-14 68.9
MS.gene50087.t1 AT4G29180 44.706 85 34 1 80 151 568 652 3.93e-14 68.9
MS.gene50087.t1 AT4G29450 44.048 84 35 2 80 151 567 650 7.52e-14 68.2
MS.gene50087.t1 AT5G59650 51.389 72 35 0 80 151 588 659 2.31e-13 66.6
MS.gene50087.t1 AT5G59650 51.389 72 35 0 80 151 580 651 2.49e-13 66.6
MS.gene50087.t1 AT5G16900 49.153 59 30 0 80 138 574 632 6.50e-13 65.5
MS.gene50087.t1 AT5G28680 44.595 74 39 1 80 151 520 593 6.58e-13 65.5
MS.gene50087.t1 AT5G28680 44.595 74 39 1 80 151 520 593 6.58e-13 65.5
MS.gene50087.t1 AT3G04690 44.595 74 39 1 80 151 516 589 1.04e-12 64.7
MS.gene50087.t1 AT1G51620 47.887 71 36 1 81 151 49 118 2.25e-12 63.5
MS.gene50087.t1 AT4G39110 40.541 74 42 1 80 151 525 598 4.27e-12 63.2
MS.gene50087.t1 AT4G32710 42.857 70 40 0 82 151 392 461 1.86e-11 61.2
MS.gene50087.t1 AT5G61350 41.892 74 41 1 80 151 524 597 3.00e-11 60.8
MS.gene50087.t1 AT1G67720 38.889 72 44 0 80 151 607 678 6.30e-11 59.7
MS.gene50087.t1 AT2G21480 34.940 83 53 1 70 151 515 597 6.44e-11 59.7
MS.gene50087.t1 AT3G24540 41.538 65 38 0 87 151 191 255 9.28e-11 59.3

Find 35 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAACTTCCAGCGGTTTAAT+TGG 0.192363 4.3:-90149799 MS.gene50087:intron
AAATACTTGACAGGTCTTAT+AGG 0.242464 4.3:-90148074 MS.gene50087:CDS
TTAATTGGAACTATAGCTTC+TGG 0.326254 4.3:-90149784 MS.gene50087:CDS
AATACTTGACAGGTCTTATA+GGG 0.376188 4.3:-90148073 MS.gene50087:CDS
TACCAACATAGCTGCAGTTT+AGG 0.411492 4.3:+90149917 None:intergenic
CTTATGGGATCAAGAGAAAC+TGG 0.413837 4.3:-90149980 MS.gene50087:CDS
CGTAGCTGAGATAGGAAATC+AGG 0.428852 4.3:+90149622 None:intergenic
TTTGAAAAGGTTGTGGAGAA+AGG 0.428970 4.3:-90148345 MS.gene50087:CDS
CAGAAACTTTGAAAAGGTTG+TGG 0.430911 4.3:-90148352 MS.gene50087:CDS
ATATTCTATTGACTTCAGAT+TGG 0.437899 4.3:+90149756 None:intergenic
TAATTGGAACTATAGCTTCT+GGG 0.444237 4.3:-90149783 MS.gene50087:CDS
TCAAGAAGCGTAGCTGAGAT+AGG 0.450478 4.3:+90149614 None:intergenic
GTTGTGGAGAAAGGAGCATC+TGG 0.469808 4.3:-90148336 MS.gene50087:CDS
CTTATAGGGTATTGTGATGA+TGG 0.472954 4.3:-90148059 MS.gene50087:CDS
GGCCTAAACTGCAGCTATGT+TGG 0.481031 4.3:-90149919 MS.gene50087:CDS
TTGTCGAATAACAATTTGAC+TGG 0.486605 4.3:-90149652 MS.gene50087:CDS
CTGGAACAGTCTACCATGGC+TGG 0.488403 4.3:-90148317 MS.gene50087:CDS
GTACTCCAGTGTCTTACTTG+TGG 0.508313 4.3:-90149945 MS.gene50087:CDS
CATTACCAGAAACTTTGAAA+AGG 0.512003 4.3:-90148358 MS.gene50087:intron
ACTTACAAGTATATGATCCT+TGG 0.514030 4.3:+90149886 None:intergenic
AAGTAAGACACTGGAGTACA+AGG 0.521279 4.3:+90149949 None:intergenic
ACTCTAGATTAGAGCCATGT+TGG 0.527280 4.3:+90148034 None:intergenic
GTTCATCATAAATACTTGAC+AGG 0.530148 4.3:-90148083 MS.gene50087:CDS
CCAGTGTCTTACTTGTGGAA+TGG 0.545016 4.3:-90149940 MS.gene50087:CDS
CAGATATCCTTGAGTTGATG+AGG 0.561128 4.3:+90148254 None:intergenic
TGGGATCAAGAGAAACTGGC+AGG 0.578914 4.3:-90149976 MS.gene50087:CDS
CTATCTTCCTCATCAACTCA+AGG 0.579253 4.3:-90148261 MS.gene50087:CDS
AAACTGCAGCTATGTTGGTA+CGG 0.585202 4.3:-90149914 MS.gene50087:CDS
CCATTCCACAAGTAAGACAC+TGG 0.617935 4.3:+90149940 None:intergenic
TATCATGATCAATCCAGCCA+TGG 0.635701 4.3:+90148304 None:intergenic
ATATCTGCAATTTCAAGCAG+AGG 0.641697 4.3:-90148238 MS.gene50087:intron
GCATCTGGAACAGTCTACCA+TGG 0.666857 4.3:-90148321 MS.gene50087:CDS
ATGTTGGTACGGATTCGCCA+AGG 0.677061 4.3:-90149903 MS.gene50087:CDS
CTGGATTGATCATGATACTG+AGG 0.708911 4.3:-90148298 MS.gene50087:CDS
TTGTGATGATGGCACCAACA+TGG 0.719558 4.3:-90148048 MS.gene50087:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAATAGATGATTTAAT+TGG - chr4.3:90149426-90149445 MS.gene50087:intron 10.0%
!! ATTTATATATCAAAAATGTA+TGG - chr4.3:90149146-90149165 MS.gene50087:intron 10.0%
!! TAAAAATAGATGATTTAATT+GGG - chr4.3:90149427-90149446 MS.gene50087:intron 10.0%
!!! TTTTATTTCTTCTTTATATT+TGG - chr4.3:90148950-90148969 MS.gene50087:intron 10.0%
!! ATAATTTGACAATTTATGAT+CGG - chr4.3:90149555-90149574 MS.gene50087:intron 15.0%
!!! TCTATTTTTATTCTATTGTT+CGG + chr4.3:90149417-90149436 None:intergenic 15.0%
!! AAAACTATACAAATTATGCA+AGG + chr4.3:90148655-90148674 None:intergenic 20.0%
!! AACTTTAAATAGAACATACT+TGG + chr4.3:90149069-90149088 None:intergenic 20.0%
!! TAGCCAAGTAATATATTAAT+GGG + chr4.3:90148691-90148710 None:intergenic 20.0%
!! TCAATTAATTATATTGCTTG+AGG - chr4.3:90148477-90148496 MS.gene50087:intron 20.0%
!!! TTTTATTTACTGAAGAAACT+TGG - chr4.3:90148552-90148571 MS.gene50087:intron 20.0%
! ACAATTTATGATCGGTAAAT+TGG - chr4.3:90149563-90149582 MS.gene50087:intron 25.0%
! AGCCAAGTAATATATTAATG+GGG + chr4.3:90148690-90148709 None:intergenic 25.0%
! GATCACAAAATAGAATGTTA+AGG - chr4.3:90148337-90148356 MS.gene50087:CDS 25.0%
! GTAGCCAAGTAATATATTAA+TGG + chr4.3:90148692-90148711 None:intergenic 25.0%
! TACCCCATTAATATATTACT+TGG - chr4.3:90148685-90148704 MS.gene50087:intron 25.0%
! TCATATACTTGTAAGTTATG+TGG - chr4.3:90148135-90148154 MS.gene50087:intron 25.0%
!! AAGCTAATACAATTTTTACG+AGG + chr4.3:90148979-90148998 None:intergenic 25.0%
!! AGCTAATACAATTTTTACGA+GGG + chr4.3:90148978-90148997 None:intergenic 25.0%
!! TAAGATGATGATGTGTAATT+AGG + chr4.3:90149489-90149508 None:intergenic 25.0%
!! TTGTCGCTTCATTAAATATA+AGG - chr4.3:90148864-90148883 MS.gene50087:intron 25.0%
!!! ATATTCTATTGACTTCAGAT+TGG + chr4.3:90148261-90148280 None:intergenic 25.0%
!!! CAAGGTTTATTTTTCTTTTG+GGG + chr4.3:90148637-90148656 None:intergenic 25.0%
!!! GCAAGGTTTATTTTTCTTTT+GGG + chr4.3:90148638-90148657 None:intergenic 25.0%
!!! GTGTGATTTTTAAGATAATC+AGG - chr4.3:90149510-90149529 MS.gene50087:intron 25.0%
!!! TGCAAGGTTTATTTTTCTTT+TGG + chr4.3:90148639-90148658 None:intergenic 25.0%
ACTTACAAGTATATGATCCT+TGG + chr4.3:90148131-90148150 None:intergenic 30.0%
ATCTTCATCTAAGTTATCCT+AGG - chr4.3:90148441-90148460 MS.gene50087:intron 30.0%
ATGAACTACAGCAAAAAGTT+TGG + chr4.3:90149917-90149936 None:intergenic 30.0%
ATTCATGCAACAAGAGAAAT+GGG - chr4.3:90149253-90149272 MS.gene50087:intron 30.0%
ATTTACTGAAGAAACTTGGA+AGG - chr4.3:90148556-90148575 MS.gene50087:intron 30.0%
GTTCATCATAAATACTTGAC+AGG - chr4.3:90149931-90149950 MS.gene50087:CDS 30.0%
TAATTGGAACTATAGCTTCT+GGG - chr4.3:90148231-90148250 MS.gene50087:intron 30.0%
TTAATTGGAACTATAGCTTC+TGG - chr4.3:90148230-90148249 MS.gene50087:intron 30.0%
TTGTCGAATAACAATTTGAC+TGG - chr4.3:90148362-90148381 MS.gene50087:intron 30.0%
! AAATACTTGACAGGTCTTAT+AGG - chr4.3:90149940-90149959 MS.gene50087:CDS 30.0%
! AATACTTGACAGGTCTTATA+GGG - chr4.3:90149941-90149960 MS.gene50087:CDS 30.0%
! ATTACTGTATGTGCAAGTTT+TGG - chr4.3:90149204-90149223 MS.gene50087:intron 30.0%
! CATTACCAGAAACTTTGAAA+AGG - chr4.3:90149656-90149675 MS.gene50087:CDS 30.0%
! CTAATACAATTTTTACGAGG+GGG + chr4.3:90148976-90148995 None:intergenic 30.0%
! GCTAATACAATTTTTACGAG+GGG + chr4.3:90148977-90148996 None:intergenic 30.0%
! GCTACTCTAAACAATTTTGT+TGG - chr4.3:90148707-90148726 MS.gene50087:intron 30.0%
ACTAACACATCACGTACAAA+AGG + chr4.3:90149116-90149135 None:intergenic 35.0%
AGTTCCTGAAGGATTATCAT+CGG + chr4.3:90148181-90148200 None:intergenic 35.0%
ATATACTACAAGAGACACCA+AGG - chr4.3:90149354-90149373 MS.gene50087:intron 35.0%
ATATCTGCAATTTCAAGCAG+AGG - chr4.3:90149776-90149795 MS.gene50087:CDS 35.0%
CTTGTGAACTGAAGGAATTA+TGG - chr4.3:90149870-90149889 MS.gene50087:intron 35.0%
GATTCATGCAACAAGAGAAA+TGG - chr4.3:90149252-90149271 MS.gene50087:intron 35.0%
GCTTTCTATTCCATTGACAT+TGG - chr4.3:90149385-90149404 MS.gene50087:intron 35.0%
GTTCCATTAGTAGTATCAGT+TGG - chr4.3:90149288-90149307 MS.gene50087:intron 35.0%
TACTATTCTTTGAACGCACA+AGG + chr4.3:90149037-90149056 None:intergenic 35.0%
TATAGTTCCAATTAAACCGC+TGG + chr4.3:90148225-90148244 None:intergenic 35.0%
TCTATTCCATTGACATTGGT+AGG - chr4.3:90149389-90149408 MS.gene50087:intron 35.0%
TTAACTTCCAGCGGTTTAAT+TGG - chr4.3:90148215-90148234 MS.gene50087:intron 35.0%
TTGAATAATCAAGTCAGCCT+AGG + chr4.3:90148461-90148480 None:intergenic 35.0%
TTTGAAAAGGTTGTGGAGAA+AGG - chr4.3:90149669-90149688 MS.gene50087:intron 35.0%
! CACAACCTTTTCAAAGTTTC+TGG + chr4.3:90149664-90149683 None:intergenic 35.0%
! CAGAAACTTTGAAAAGGTTG+TGG - chr4.3:90149662-90149681 MS.gene50087:intron 35.0%
!! CTTATAGGGTATTGTGATGA+TGG - chr4.3:90149955-90149974 MS.gene50087:CDS 35.0%
AAACTGCAGCTATGTTGGTA+CGG - chr4.3:90148100-90148119 MS.gene50087:CDS 40.0%
AAGGAAATCAGCTATCAGCA+GGG - chr4.3:90148575-90148594 MS.gene50087:intron 40.0%
AAGTAAGACACTGGAGTACA+AGG + chr4.3:90148068-90148087 None:intergenic 40.0%
ACGATGTTCTTGTGAACTGA+AGG - chr4.3:90149862-90149881 MS.gene50087:intron 40.0%
ATAGAAAGCCAAACCTACCT+TGG + chr4.3:90149374-90149393 None:intergenic 40.0%
ATGCTACCCCATTGTTATGA+AGG + chr4.3:90148608-90148627 None:intergenic 40.0%
CAACAAGAGAAATGGGAGTA+AGG - chr4.3:90149260-90149279 MS.gene50087:intron 40.0%
CCACCAACTGATACTACTAA+TGG + chr4.3:90149294-90149313 None:intergenic 40.0%
CCATTAGTAGTATCAGTTGG+TGG - chr4.3:90149291-90149310 MS.gene50087:intron 40.0%
CGAATCCCTTCATAACAATG+GGG - chr4.3:90148599-90148618 MS.gene50087:intron 40.0%
CTATCTTCCTCATCAACTCA+AGG - chr4.3:90149753-90149772 MS.gene50087:intron 40.0%
CTGGATTGATCATGATACTG+AGG - chr4.3:90149716-90149735 MS.gene50087:intron 40.0%
GCGAATCCCTTCATAACAAT+GGG - chr4.3:90148598-90148617 MS.gene50087:intron 40.0%
TACCAACATAGCTGCAGTTT+AGG + chr4.3:90148100-90148119 None:intergenic 40.0%
TATCATGATCAATCCAGCCA+TGG + chr4.3:90149713-90149732 None:intergenic 40.0%
TCATAACAATGGGGTAGCAT+TGG - chr4.3:90148608-90148627 MS.gene50087:intron 40.0%
TCTCTTGTTGCATGAATCTG+AGG + chr4.3:90149250-90149269 None:intergenic 40.0%
TGCTACCCCATTGTTATGAA+GGG + chr4.3:90148607-90148626 None:intergenic 40.0%
! GGTTTTCCTTTCTGATTCAC+AGG - chr4.3:90149891-90149910 MS.gene50087:intron 40.0%
!! CAGATATCCTTGAGTTGATG+AGG + chr4.3:90149763-90149782 None:intergenic 40.0%
!! TTCTGGAACTTAACTTCCAG+CGG - chr4.3:90148206-90148225 MS.gene50087:intron 40.0%
AAGAGACACCAAGGTAGGTT+TGG - chr4.3:90149363-90149382 MS.gene50087:intron 45.0%
ACTACAAGAGACACCAAGGT+AGG - chr4.3:90149358-90149377 MS.gene50087:intron 45.0%
AGGAAATCAGCTATCAGCAG+GGG - chr4.3:90148576-90148595 MS.gene50087:intron 45.0%
CCAGAAGCTATAGTTCCTGA+AGG + chr4.3:90148192-90148211 None:intergenic 45.0%
CCAGTGTCTTACTTGTGGAA+TGG - chr4.3:90148074-90148093 MS.gene50087:CDS 45.0%
CCATTCCACAAGTAAGACAC+TGG + chr4.3:90148077-90148096 None:intergenic 45.0%
CCTTCAGGAACTATAGCTTC+TGG - chr4.3:90148189-90148208 MS.gene50087:intron 45.0%
CGTAGCTGAGATAGGAAATC+AGG + chr4.3:90148395-90148414 None:intergenic 45.0%
GAACGCACAAGGAGTTGTAT+TGG + chr4.3:90149026-90149045 None:intergenic 45.0%
GAAGGAAATCAGCTATCAGC+AGG - chr4.3:90148574-90148593 MS.gene50087:intron 45.0%
GAATTGATCCTGATAGCCAG+CGG + chr4.3:90149004-90149023 None:intergenic 45.0%
GGCGAATCCCTTCATAACAA+TGG - chr4.3:90148597-90148616 MS.gene50087:intron 45.0%
GTACTCCAGTGTCTTACTTG+TGG - chr4.3:90148069-90148088 MS.gene50087:CDS 45.0%
GTTTGGCCTGTGAATCAGAA+AGG + chr4.3:90149900-90149919 None:intergenic 45.0%
TACGCCGATGATAATCCTTC+AGG - chr4.3:90148174-90148193 MS.gene50087:intron 45.0%
! AATTGTATTAGCTTCGCCGC+TGG - chr4.3:90148985-90149004 MS.gene50087:intron 45.0%
! TCAAGAAGCGTAGCTGAGAT+AGG + chr4.3:90148403-90148422 None:intergenic 45.0%
!! TTGTGATGATGGCACCAACA+TGG - chr4.3:90149966-90149985 MS.gene50087:CDS 45.0%
ATGTTGGTACGGATTCGCCA+AGG - chr4.3:90148111-90148130 MS.gene50087:intron 50.0%
GGCCTAAACTGCAGCTATGT+TGG - chr4.3:90148095-90148114 MS.gene50087:CDS 50.0%
TCGGCTCCTACCAATGTCAA+TGG + chr4.3:90149398-90149417 None:intergenic 50.0%
TGGGATCAAGAGAAACTGGC+AGG - chr4.3:90148038-90148057 MS.gene50087:CDS 50.0%
! GCATCTGGAACAGTCTACCA+TGG - chr4.3:90149693-90149712 MS.gene50087:intron 50.0%
!! GTTGTGGAGAAAGGAGCATC+TGG - chr4.3:90149678-90149697 MS.gene50087:intron 50.0%
CTGGAACAGTCTACCATGGC+TGG - chr4.3:90149697-90149716 MS.gene50087:intron 55.0%
! TAGCTTCGCCGCTGGCTATC+AGG - chr4.3:90148993-90149012 MS.gene50087:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 90148037 90149999 90148037 ID=MS.gene50087
chr4.3 mRNA 90148037 90149999 90148037 ID=MS.gene50087.t1;Parent=MS.gene50087
chr4.3 exon 90149892 90149999 90149892 ID=MS.gene50087.t1.exon1;Parent=MS.gene50087.t1
chr4.3 CDS 90149892 90149999 90149892 ID=cds.MS.gene50087.t1;Parent=MS.gene50087.t1
chr4.3 exon 90149754 90149810 90149754 ID=MS.gene50087.t1.exon2;Parent=MS.gene50087.t1
chr4.3 CDS 90149754 90149810 90149754 ID=cds.MS.gene50087.t1;Parent=MS.gene50087.t1
chr4.3 exon 90149607 90149677 90149607 ID=MS.gene50087.t1.exon3;Parent=MS.gene50087.t1
chr4.3 CDS 90149607 90149677 90149607 ID=cds.MS.gene50087.t1;Parent=MS.gene50087.t1
chr4.3 exon 90148239 90148371 90148239 ID=MS.gene50087.t1.exon4;Parent=MS.gene50087.t1
chr4.3 CDS 90148239 90148371 90148239 ID=cds.MS.gene50087.t1;Parent=MS.gene50087.t1
chr4.3 exon 90148037 90148123 90148037 ID=MS.gene50087.t1.exon5;Parent=MS.gene50087.t1
chr4.3 CDS 90148037 90148123 90148037 ID=cds.MS.gene50087.t1;Parent=MS.gene50087.t1
Gene Sequence

>MS.gene50087

ATGGGATCAAGAGAAACTGGCAGGAGATCCTTGTACTCCAGTGTCTTACTTGTGGAATGGCCTAAACTGCAGCTATGTTGGTACGGATTCGCCAAGGATCATATACTTGTAAGTTATGTGGCATGAAATGAAATGCATACGCCGATGATAATCCTTCAGGAACTATAGCTTCTGGAACTTAACTTCCAGCGGTTTAATTGGAACTATAGCTTCTGGGATATCCAATCTGAAGTCAATAGAATATTTGTAAGTTCTTGAATCTATTATGTACACGAAAAACAAATAGCAAGCTAATAAGTTGATCACAAAATAGAATGTTAAGGTTTTGTCGAATAACAATTTGACTGGTGCTGTGCCTGATTTCCTATCTCAGCTACGCTTCTTGAGAGTTCTGTAAGTCAACTATCTTCATCTAAGTTATCCTAGGCTGACTTGATTATTCAATTAATTATATTGCTTGAGGTGCTCAAAATATATTCTTGTCGAGAATGAAAGCATGTAAAGAGTAATTACTTTTTTATTTACTGAAGAAACTTGGAAGGAAATCAGCTATCAGCAGGGGCGAATCCCTTCATAACAATGGGGTAGCATTGGCTACCCCAAAAGAAAAATAAACCTTGCATAATTTGTATAGTTTTAAAATATAGCTACCCCATTAATATATTACTTGGCTACTCTAAACAATTTTGTTGGTTTAAAGAGAGATTATTTAAAGAAAATACTTATAATCTATTTAGTTTAGTGTCAATTATAAATAAATTTGACTTGTTAAAACTTCATAGAGTACAAATTTTATAAATAATTATTGTTAGATTTTATTTTCTTCGTTGTCGCTTCATTAAATATAAGGAAAATTTATTGCAAACTTATTTTACTTATTAATTAAGTTTAATATATTAATTTTTAAAATATATTTTATTTCTTCTTTATATTTGGCCCCCTCGTAAAAATTGTATTAGCTTCGCCGCTGGCTATCAGGATCAATTCCAATACAACTCCTTGTGCGTTCAAAGAATAGTATGCTCGAATCCAAGTATGTTCTATTTAAAGTTTTACTTTTACATATTTTATGACTTCCTTTTGTACGTGATGTGTTAGTGATTTATCTCATTTATATATCAAAAATGTATGGTGTCAGACTATGTCAAATAAATAACATGATTATATATTACTGTATGTGCAAGTTTTGGTAGAAATTCAAATCTTTGCACCTCAGATTCATGCAACAAGAGAAATGGGAGTAAGGTTTTAGTTCCATTAGTAGTATCAGTTGGTGGAGCTTTCATTATACTAGTAGTAGCAATGATTTCTTTTCGCATATACTACAAGAGACACCAAGGTAGGTTTGGCTTTCTATTCCATTGACATTGGTAGGAGCCGAACAATAGAATAAAAATAGATGATTTAATTGGGTATGCAATTTAAATGTAAAAATAATTTACACTGTCGCCTAATTACACATCATCATCTTAAGTGTGATTTTTAAGATAATCAGGATCGAAGTTAACATTATTTTTAATAATTTGACAATTTATGATCGGTAAATTGGTTGACTACACTCAACGTGTATAAAAATTAGAGTCAAAGAAACAAGAATTCAGCTATGAAGAAGTGTTAAACATTACCAGAAACTTTGAAAAGGTTGTGGAGAAAGGAGCATCTGGAACAGTCTACCATGGCTGGATTGATCATGATACTGAGGTAGCTGTCAAAATGCTATCTTCCTCATCAACTCAAGGATATCTGCAATTTCAAGCAGAGGTAGTATCATGAATTAATTAATTTTCAAATTTTATCATGTTTATTCGCACATATTATAGCTTCAACGATGTTCTTGTGAACTGAAGGAATTATGGTTTTCCTTTCTGATTCACAGGCCAAACTTTTTGCTGTAGTTCATCATAAATACTTGACAGGTCTTATAGGGTATTGTGATGATGGCACCAACATGGCTCTAATCTAG
Protein sequence

>MS.gene50087.t1

MGSRETGRRSLYSSVLLVEWPKLQLCWYGFAKDHILRFNWNYSFWDIQSEVNRIFVLSNNNLTGAVPDFLSQLRFLRVLNFEKVVEKGASGTVYHGWIDHDTEVAVKMLSSSSTQGYLQFQAEAKLFAVVHHKYLTGLIGYCDDGTNMALI