Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50137.t1 | XP_003616466.2 | 71.9 | 235 | 5 | 3 | 1 | 175 | 1 | 234 | 1.20E-82 | 315.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50137.t1 | O80942 | 64.3 | 70 | 19 | 2 | 11 | 80 | 7 | 70 | 8.1e-19 | 95.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50137.t1 | G7KGU7 | 71.9 | 235 | 5 | 3 | 1 | 175 | 1 | 234 | 8.8e-83 | 315.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene50137.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50137.t1 | MTR_5g080660 | 68.376 | 234 | 15 | 3 | 1 | 175 | 1 | 234 | 9.16e-107 | 305 |
| MS.gene50137.t1 | MTR_3g068095 | 52.500 | 120 | 41 | 3 | 11 | 115 | 8 | 126 | 5.56e-31 | 113 |
| MS.gene50137.t1 | MTR_3g068095 | 37.821 | 156 | 68 | 9 | 40 | 173 | 112 | 260 | 1.97e-11 | 61.2 |
| MS.gene50137.t1 | MTR_4g021270 | 71.014 | 69 | 16 | 1 | 23 | 91 | 35 | 99 | 5.01e-28 | 107 |
| MS.gene50137.t1 | MTR_5g014400 | 64.474 | 76 | 24 | 3 | 10 | 84 | 44 | 117 | 3.08e-27 | 103 |
| MS.gene50137.t1 | MTR_1g070265 | 59.701 | 67 | 26 | 1 | 41 | 106 | 29 | 95 | 1.55e-19 | 81.6 |
| MS.gene50137.t1 | MTR_2g076060 | 66.667 | 48 | 15 | 1 | 38 | 84 | 40 | 87 | 2.25e-18 | 79.3 |
| MS.gene50137.t1 | MTR_4g064873 | 73.684 | 38 | 10 | 0 | 46 | 83 | 20 | 57 | 1.66e-14 | 68.2 |
| MS.gene50137.t1 | MTR_7g063220 | 37.302 | 126 | 53 | 3 | 47 | 170 | 24 | 125 | 3.98e-14 | 66.2 |
| MS.gene50137.t1 | MTR_1g070250 | 45.833 | 72 | 32 | 2 | 43 | 107 | 34 | 105 | 3.43e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50137.t1 | AT2G37740 | 64.286 | 70 | 19 | 2 | 11 | 80 | 7 | 70 | 1.24e-25 | 100 |
| MS.gene50137.t1 | AT4G17810 | 62.319 | 69 | 23 | 2 | 10 | 76 | 6 | 73 | 7.10e-25 | 95.1 |
| MS.gene50137.t1 | AT4G17810 | 62.319 | 69 | 23 | 2 | 10 | 76 | 6 | 73 | 1.25e-24 | 95.5 |
| MS.gene50137.t1 | AT5G06070 | 55.952 | 84 | 26 | 2 | 6 | 88 | 22 | 95 | 6.61e-23 | 91.3 |
| MS.gene50137.t1 | AT3G23130 | 76.744 | 43 | 10 | 0 | 37 | 79 | 35 | 77 | 1.90e-18 | 79.3 |
| MS.gene50137.t1 | AT2G42410 | 32.184 | 174 | 64 | 5 | 44 | 168 | 44 | 212 | 2.46e-17 | 76.3 |
| MS.gene50137.t1 | AT2G42410 | 32.184 | 174 | 64 | 5 | 44 | 168 | 44 | 212 | 2.46e-17 | 76.3 |
| MS.gene50137.t1 | AT2G42410 | 32.184 | 174 | 64 | 5 | 44 | 168 | 70 | 238 | 4.32e-17 | 76.3 |
| MS.gene50137.t1 | AT3G09290 | 32.099 | 162 | 67 | 5 | 39 | 175 | 25 | 168 | 7.06e-15 | 68.9 |
| MS.gene50137.t1 | AT3G53820 | 50.725 | 69 | 30 | 3 | 40 | 106 | 14 | 80 | 1.09e-13 | 65.1 |
| MS.gene50137.t1 | AT5G43540 | 33.333 | 126 | 56 | 4 | 48 | 168 | 34 | 136 | 8.25e-11 | 57.4 |
Find 32 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCTGATCAGTACTGGTTT+TGG | 0.291198 | 5.4:+63202950 | MS.gene50137:CDS |
| ACTATTCCTAGTAAGGAAAA+AGG | 0.312548 | 5.4:+63203450 | MS.gene50137:CDS |
| AATTTGTCTTTAGGAATGTT+TGG | 0.353496 | 5.4:+63203414 | MS.gene50137:intron |
| TTAGATCTTGAACTCAGGTT+GGG | 0.396208 | 5.4:+63203621 | MS.gene50137:CDS |
| AAATGATCATCATAGGTTCC+TGG | 0.401685 | 5.4:+63203545 | MS.gene50137:CDS |
| TTTAGGTCTGCTCAAGCTCT+TGG | 0.423987 | 5.4:+63203108 | MS.gene50137:CDS |
| ATCATCAACTATTCCTAGTA+AGG | 0.427141 | 5.4:+63203443 | MS.gene50137:CDS |
| CTGAAGGTTGTTGAAAAGCC+AGG | 0.429995 | 5.4:-63203563 | None:intergenic |
| CAACCTTCAGAAATTGTACT+AGG | 0.453879 | 5.4:+63203576 | MS.gene50137:CDS |
| TTCAAGCACCAATTGAATCA+TGG | 0.457719 | 5.4:+63202989 | MS.gene50137:CDS |
| AATTGAATCATGGGAAGAAA+AGG | 0.467520 | 5.4:+63202999 | MS.gene50137:CDS |
| ATCAAATCATTGTTCTTCTG+AGG | 0.467552 | 5.4:-63203186 | None:intergenic |
| TTTAGATCTTGAACTCAGGT+TGG | 0.474254 | 5.4:+63203620 | MS.gene50137:CDS |
| GATCTTACTCTGATCAGTAC+TGG | 0.491061 | 5.4:+63202944 | MS.gene50137:CDS |
| TCAAGCACCAATTGAATCAT+GGG | 0.500236 | 5.4:+63202990 | MS.gene50137:CDS |
| AAGATTTAGATCTTGAACTC+AGG | 0.503939 | 5.4:+63203616 | MS.gene50137:CDS |
| AAGTTACATGAATAAAATCT+TGG | 0.505499 | 5.4:-63203072 | None:intergenic |
| CATGTTCAAATGATCATCAT+AGG | 0.515352 | 5.4:+63203538 | MS.gene50137:CDS |
| AGGTCTGCTCAAGCTCTTGG+TGG | 0.535050 | 5.4:+63203111 | MS.gene50137:CDS |
| TAGATCTTGAACTCAGGTTG+GGG | 0.538027 | 5.4:+63203622 | MS.gene50137:CDS |
| TCAAATCATTGTTCTTCTGA+GGG | 0.542057 | 5.4:-63203185 | None:intergenic |
| GTGGTCACATGAATATTCAT+AGG | 0.543934 | 5.4:+63203130 | MS.gene50137:CDS |
| ATTGTACTAGGACTTGATCA+TGG | 0.568821 | 5.4:+63203588 | MS.gene50137:CDS |
| TTACATGAATAAAATCTTGG+TGG | 0.571433 | 5.4:-63203069 | None:intergenic |
| GCAGCTAGGAATATACTGAA+TGG | 0.590719 | 5.4:+63203036 | MS.gene50137:CDS |
| AGTCCTAGTACAATTTCTGA+AGG | 0.591874 | 5.4:-63203579 | None:intergenic |
| TTCATAGGAGAGATAGAGCT+AGG | 0.597256 | 5.4:+63203145 | MS.gene50137:CDS |
| ACTAGGACTTGATCATGGAA+TGG | 0.618438 | 5.4:+63203593 | MS.gene50137:CDS |
| GATTTGATCAACTCATCACT+AGG | 0.628308 | 5.4:+63203201 | MS.gene50137:CDS |
| GATCATTTGAACATGTCTTG+AGG | 0.638691 | 5.4:-63203530 | None:intergenic |
| CATGTCTTGAGGAACACAGA+AGG | 0.671603 | 5.4:-63203519 | None:intergenic |
| ATATACTGAATGGTAGCATG+TGG | 0.674162 | 5.4:+63203046 | MS.gene50137:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGATAATTACAATCTATTTT+TGG | - | chr5.4:63203255-63203274 | None:intergenic | 15.0% |
| !! | AAGTTACATGAATAAAATCT+TGG | - | chr5.4:63203075-63203094 | None:intergenic | 20.0% |
| ! | GTTGAAACAAATTTGTCTTT+AGG | + | chr5.4:63203405-63203424 | MS.gene50137:intron | 25.0% |
| ! | TTACATGAATAAAATCTTGG+TGG | - | chr5.4:63203072-63203091 | None:intergenic | 25.0% |
| ! | TTAGTTGAGATAATTCTAGA+AGG | - | chr5.4:63203342-63203361 | None:intergenic | 25.0% |
| !! | AATTTGTCTTTAGGAATGTT+TGG | + | chr5.4:63203414-63203433 | MS.gene50137:intron | 25.0% |
| !! | ACTTTTGTAAAAGAGAGTTT+AGG | + | chr5.4:63203091-63203110 | MS.gene50137:CDS | 25.0% |
| !! | CTTCTTCTAGTTGTAATTAA+GGG | - | chr5.4:63203309-63203328 | None:intergenic | 25.0% |
| !! | TCTTCTTCTAGTTGTAATTA+AGG | - | chr5.4:63203310-63203329 | None:intergenic | 25.0% |
| AAGATTTAGATCTTGAACTC+AGG | + | chr5.4:63203616-63203635 | MS.gene50137:CDS | 30.0% | |
| AATTGAATCATGGGAAGAAA+AGG | + | chr5.4:63202999-63203018 | MS.gene50137:CDS | 30.0% | |
| ACTATTCCTAGTAAGGAAAA+AGG | + | chr5.4:63203450-63203469 | MS.gene50137:CDS | 30.0% | |
| ATCAAATCATTGTTCTTCTG+AGG | - | chr5.4:63203189-63203208 | None:intergenic | 30.0% | |
| ATCATCAACTATTCCTAGTA+AGG | + | chr5.4:63203443-63203462 | MS.gene50137:CDS | 30.0% | |
| CATGTTCAAATGATCATCAT+AGG | + | chr5.4:63203538-63203557 | MS.gene50137:CDS | 30.0% | |
| TCAAATCATTGTTCTTCTGA+GGG | - | chr5.4:63203188-63203207 | None:intergenic | 30.0% | |
| ! | ATGATACCTTTTTCCTTACT+AGG | - | chr5.4:63203459-63203478 | None:intergenic | 30.0% |
| ! | TGCTAGAGAAGACAATTTTT+CGG | + | chr5.4:63203368-63203387 | MS.gene50137:intron | 30.0% |
| ! | TTGTAATTAAGGGTTTTGCA+GGG | - | chr5.4:63203299-63203318 | None:intergenic | 30.0% |
| ! | TTTTCTTCCCATGATTCAAT+TGG | - | chr5.4:63203000-63203019 | None:intergenic | 30.0% |
| !! | AGACAATTTTTCGGATTGTA+AGG | + | chr5.4:63203377-63203396 | MS.gene50137:intron | 30.0% |
| AAATGATCATCATAGGTTCC+TGG | + | chr5.4:63203545-63203564 | MS.gene50137:CDS | 35.0% | |
| AGTCCTAGTACAATTTCTGA+AGG | - | chr5.4:63203582-63203601 | None:intergenic | 35.0% | |
| ATATACTGAATGGTAGCATG+TGG | + | chr5.4:63203046-63203065 | MS.gene50137:CDS | 35.0% | |
| ATTGTACTAGGACTTGATCA+TGG | + | chr5.4:63203588-63203607 | MS.gene50137:CDS | 35.0% | |
| CAACCTTCAGAAATTGTACT+AGG | + | chr5.4:63203576-63203595 | MS.gene50137:CDS | 35.0% | |
| GATCATTTGAACATGTCTTG+AGG | - | chr5.4:63203533-63203552 | None:intergenic | 35.0% | |
| GATTTGATCAACTCATCACT+AGG | + | chr5.4:63203201-63203220 | MS.gene50137:CDS | 35.0% | |
| GTGGTCACATGAATATTCAT+AGG | + | chr5.4:63203130-63203149 | MS.gene50137:CDS | 35.0% | |
| TCAAGCACCAATTGAATCAT+GGG | + | chr5.4:63202990-63203009 | MS.gene50137:CDS | 35.0% | |
| TTAGATCTTGAACTCAGGTT+GGG | + | chr5.4:63203621-63203640 | MS.gene50137:CDS | 35.0% | |
| TTCAAGCACCAATTGAATCA+TGG | + | chr5.4:63202989-63203008 | MS.gene50137:CDS | 35.0% | |
| TTTAGATCTTGAACTCAGGT+TGG | + | chr5.4:63203620-63203639 | MS.gene50137:CDS | 35.0% | |
| ! | AGATAATTCTAGAAGGTGAG+AGG | - | chr5.4:63203335-63203354 | None:intergenic | 35.0% |
| ! | GATAATTCTAGAAGGTGAGA+GGG | - | chr5.4:63203334-63203353 | None:intergenic | 35.0% |
| ! | GTTGTAATTAAGGGTTTTGC+AGG | - | chr5.4:63203300-63203319 | None:intergenic | 35.0% |
| ACTAGGACTTGATCATGGAA+TGG | + | chr5.4:63203593-63203612 | MS.gene50137:CDS | 40.0% | |
| GATCTTACTCTGATCAGTAC+TGG | + | chr5.4:63202944-63202963 | MS.gene50137:CDS | 40.0% | |
| GCAGCTAGGAATATACTGAA+TGG | + | chr5.4:63203036-63203055 | MS.gene50137:CDS | 40.0% | |
| TTCATAGGAGAGATAGAGCT+AGG | + | chr5.4:63203145-63203164 | MS.gene50137:CDS | 40.0% | |
| ! | TAGATCTTGAACTCAGGTTG+GGG | + | chr5.4:63203622-63203641 | MS.gene50137:CDS | 40.0% |
| !!! | ACTCTGATCAGTACTGGTTT+TGG | + | chr5.4:63202950-63202969 | MS.gene50137:CDS | 40.0% |
| !!! | ACTGGTTTTGGATGAAGAGA+AGG | + | chr5.4:63202962-63202981 | MS.gene50137:CDS | 40.0% |
| CATGTCTTGAGGAACACAGA+AGG | - | chr5.4:63203522-63203541 | None:intergenic | 45.0% | |
| CTGAAGGTTGTTGAAAAGCC+AGG | - | chr5.4:63203566-63203585 | None:intergenic | 45.0% | |
| TTTAGGTCTGCTCAAGCTCT+TGG | + | chr5.4:63203108-63203127 | MS.gene50137:CDS | 45.0% | |
| ! | CTTTTGCAGAAGATGCAGCT+AGG | + | chr5.4:63203022-63203041 | MS.gene50137:CDS | 45.0% |
| ! | GGTTTTGCAGGGCTAGAACA+AGG | - | chr5.4:63203288-63203307 | None:intergenic | 50.0% |
| AGGTCTGCTCAAGCTCTTGG+TGG | + | chr5.4:63203111-63203130 | MS.gene50137:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 63202937 | 63203668 | 63202937 | ID=MS.gene50137 |
| chr5.4 | mRNA | 63202937 | 63203668 | 63202937 | ID=MS.gene50137.t1;Parent=MS.gene50137 |
| chr5.4 | exon | 63202937 | 63203222 | 63202937 | ID=MS.gene50137.t1.exon1;Parent=MS.gene50137.t1 |
| chr5.4 | CDS | 63202937 | 63203222 | 63202937 | ID=cds.MS.gene50137.t1;Parent=MS.gene50137.t1 |
| chr5.4 | exon | 63203427 | 63203668 | 63203427 | ID=MS.gene50137.t1.exon2;Parent=MS.gene50137.t1 |
| chr5.4 | CDS | 63203427 | 63203668 | 63203427 | ID=cds.MS.gene50137.t1;Parent=MS.gene50137.t1 |
| Gene Sequence |
| Protein sequence |
