Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50176.t1 | XP_003598029.2 | 67.6 | 411 | 107 | 5 | 13 | 398 | 40 | 449 | 1.70E-148 | 535.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50176.t1 | Q8L3W1 | 40.5 | 84 | 50 | 0 | 13 | 96 | 21 | 104 | 1.2e-14 | 82.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50176.t1 | G7IYE8 | 67.6 | 411 | 107 | 5 | 13 | 398 | 40 | 449 | 1.2e-148 | 535.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene50176.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50176.t1 | MTR_3g005460 | 67.640 | 411 | 107 | 5 | 13 | 398 | 40 | 449 | 0.0 | 540 |
| MS.gene50176.t1 | MTR_3g005460 | 46.025 | 239 | 79 | 4 | 13 | 202 | 277 | 514 | 9.06e-59 | 200 |
| MS.gene50176.t1 | MTR_3g005460 | 50.718 | 209 | 77 | 2 | 194 | 398 | 2 | 188 | 7.64e-55 | 189 |
| MS.gene50176.t1 | MTR_3g005420 | 65.423 | 402 | 105 | 4 | 13 | 380 | 55 | 456 | 5.86e-173 | 494 |
| MS.gene50176.t1 | MTR_3g005420 | 48.052 | 231 | 78 | 2 | 13 | 202 | 304 | 533 | 1.57e-60 | 205 |
| MS.gene50176.t1 | MTR_3g005420 | 50.777 | 193 | 67 | 5 | 212 | 398 | 35 | 205 | 2.13e-45 | 164 |
| MS.gene50176.t1 | MTR_3g005495 | 69.828 | 348 | 88 | 3 | 11 | 342 | 26 | 372 | 1.01e-170 | 486 |
| MS.gene50176.t1 | MTR_3g005495 | 47.005 | 217 | 84 | 4 | 13 | 201 | 258 | 471 | 6.88e-54 | 186 |
| MS.gene50176.t1 | MTR_3g005440 | 58.049 | 410 | 111 | 5 | 10 | 385 | 32 | 414 | 1.10e-146 | 424 |
| MS.gene50176.t1 | MTR_3g005420 | 58.209 | 402 | 99 | 5 | 13 | 380 | 36 | 402 | 3.38e-143 | 416 |
| MS.gene50176.t1 | MTR_3g005420 | 50.777 | 193 | 67 | 5 | 212 | 398 | 16 | 186 | 5.89e-46 | 165 |
| MS.gene50176.t1 | MTR_3g005420 | 45.408 | 196 | 100 | 2 | 13 | 202 | 285 | 479 | 4.47e-41 | 151 |
| MS.gene50176.t1 | MTR_5g070440 | 67.188 | 192 | 58 | 2 | 212 | 398 | 16 | 207 | 5.59e-82 | 252 |
| MS.gene50176.t1 | MTR_5g070440 | 52.381 | 231 | 68 | 2 | 13 | 202 | 36 | 265 | 1.75e-71 | 225 |
| MS.gene50176.t1 | MTR_3g005490 | 63.542 | 192 | 55 | 3 | 212 | 398 | 16 | 197 | 2.37e-75 | 235 |
| MS.gene50176.t1 | MTR_3g005490 | 52.193 | 228 | 76 | 3 | 13 | 208 | 36 | 262 | 1.97e-70 | 223 |
| MS.gene50176.t1 | MTR_3g005480 | 67.052 | 173 | 43 | 2 | 212 | 380 | 16 | 178 | 1.00e-72 | 228 |
| MS.gene50176.t1 | MTR_3g005480 | 50.862 | 232 | 80 | 3 | 4 | 202 | 27 | 257 | 1.18e-67 | 215 |
| MS.gene50176.t1 | MTR_1g015350 | 40.690 | 145 | 55 | 2 | 42 | 186 | 59 | 172 | 4.42e-22 | 92.8 |
| MS.gene50176.t1 | MTR_1g015350 | 55.696 | 79 | 22 | 1 | 256 | 334 | 58 | 123 | 9.84e-19 | 83.6 |
| MS.gene50176.t1 | MTR_1g034240 | 42.424 | 99 | 53 | 1 | 217 | 311 | 7 | 105 | 5.59e-21 | 94.7 |
| MS.gene50176.t1 | MTR_1g034240 | 40.860 | 93 | 55 | 0 | 4 | 96 | 13 | 105 | 1.83e-19 | 90.1 |
| MS.gene50176.t1 | MTR_3g061310 | 46.067 | 89 | 46 | 1 | 10 | 96 | 20 | 108 | 2.12e-18 | 85.1 |
| MS.gene50176.t1 | MTR_3g061310 | 25.824 | 182 | 108 | 3 | 216 | 370 | 11 | 192 | 3.44e-13 | 70.1 |
| MS.gene50176.t1 | MTR_3g061350 | 39.474 | 114 | 66 | 2 | 10 | 121 | 20 | 132 | 4.84e-17 | 81.3 |
| MS.gene50176.t1 | MTR_3g061350 | 30.055 | 183 | 98 | 5 | 217 | 370 | 12 | 193 | 7.18e-13 | 68.9 |
| MS.gene50176.t1 | MTR_7g050560 | 33.333 | 105 | 66 | 1 | 14 | 114 | 29 | 133 | 2.36e-16 | 79.7 |
| MS.gene50176.t1 | MTR_7g050560 | 31.156 | 199 | 105 | 8 | 216 | 383 | 12 | 209 | 1.44e-15 | 77.4 |
| MS.gene50176.t1 | MTR_3g061320 | 44.944 | 89 | 47 | 1 | 10 | 96 | 20 | 108 | 2.45e-16 | 79.0 |
| MS.gene50176.t1 | MTR_3g061320 | 32.394 | 142 | 93 | 2 | 216 | 355 | 11 | 151 | 5.71e-13 | 69.3 |
| MS.gene50176.t1 | MTR_4g068320 | 37.255 | 102 | 60 | 1 | 214 | 311 | 3 | 104 | 3.76e-16 | 80.1 |
| MS.gene50176.t1 | MTR_4g068320 | 34.615 | 104 | 68 | 0 | 1 | 104 | 9 | 112 | 4.27e-16 | 80.1 |
| MS.gene50176.t1 | MTR_7g050710 | 34.579 | 107 | 62 | 1 | 13 | 111 | 28 | 134 | 3.82e-16 | 79.3 |
| MS.gene50176.t1 | MTR_7g050710 | 31.405 | 121 | 74 | 2 | 228 | 339 | 28 | 148 | 1.12e-13 | 72.0 |
| MS.gene50176.t1 | MTR_3g061290 | 43.820 | 89 | 48 | 1 | 10 | 96 | 20 | 108 | 6.93e-16 | 76.3 |
| MS.gene50176.t1 | MTR_3g061290 | 36.522 | 115 | 70 | 2 | 216 | 328 | 11 | 124 | 4.11e-13 | 68.2 |
| MS.gene50176.t1 | MTR_1g034210 | 40.000 | 90 | 50 | 1 | 217 | 302 | 7 | 96 | 1.09e-15 | 77.8 |
| MS.gene50176.t1 | MTR_1g034210 | 40.476 | 84 | 48 | 1 | 13 | 96 | 22 | 103 | 1.03e-14 | 75.1 |
| MS.gene50176.t1 | MTR_1g034210 | 40.000 | 90 | 50 | 1 | 217 | 302 | 7 | 96 | 1.76e-15 | 77.8 |
| MS.gene50176.t1 | MTR_1g034210 | 36.458 | 96 | 59 | 1 | 1 | 96 | 10 | 103 | 1.49e-14 | 74.7 |
| MS.gene50176.t1 | MTR_3g061330 | 41.667 | 84 | 49 | 0 | 13 | 96 | 25 | 108 | 3.74e-15 | 75.1 |
| MS.gene50176.t1 | MTR_3g061330 | 28.415 | 183 | 100 | 4 | 217 | 370 | 12 | 192 | 3.76e-12 | 66.2 |
| MS.gene50176.t1 | MTR_3g061370 | 41.667 | 84 | 49 | 0 | 13 | 96 | 22 | 105 | 4.19e-15 | 75.5 |
| MS.gene50176.t1 | MTR_3g061370 | 29.861 | 144 | 99 | 1 | 214 | 355 | 6 | 149 | 1.82e-12 | 67.8 |
| MS.gene50176.t1 | MTR_7g050590 | 33.929 | 112 | 67 | 3 | 14 | 118 | 2 | 113 | 1.28e-14 | 74.3 |
| MS.gene50176.t1 | MTR_7g050590 | 31.776 | 107 | 67 | 1 | 233 | 333 | 6 | 112 | 1.03e-12 | 68.6 |
| MS.gene50176.t1 | MTR_3g061340 | 45.556 | 90 | 46 | 2 | 10 | 96 | 20 | 109 | 3.25e-14 | 72.8 |
| MS.gene50176.t1 | MTR_3g061340 | 30.688 | 189 | 110 | 7 | 217 | 386 | 12 | 198 | 1.43e-12 | 67.8 |
| MS.gene50176.t1 | MTR_7g050720 | 30.435 | 138 | 92 | 1 | 216 | 349 | 9 | 146 | 4.65e-14 | 72.8 |
| MS.gene50176.t1 | MTR_7g050720 | 32.143 | 84 | 57 | 0 | 14 | 97 | 26 | 109 | 9.63e-12 | 65.9 |
| MS.gene50176.t1 | MTR_5g044670 | 33.735 | 83 | 55 | 0 | 14 | 96 | 32 | 114 | 1.78e-13 | 71.2 |
| MS.gene50176.t1 | MTR_5g044670 | 35.106 | 94 | 54 | 1 | 218 | 311 | 28 | 114 | 4.78e-12 | 67.0 |
| MS.gene50176.t1 | MTR_3g061220 | 40.449 | 89 | 38 | 2 | 10 | 96 | 20 | 95 | 3.21e-13 | 66.2 |
| MS.gene50176.t1 | MTR_1g108780 | 30.909 | 110 | 71 | 1 | 11 | 115 | 10 | 119 | 3.43e-13 | 71.2 |
| MS.gene50176.t1 | MTR_3g098790 | 34.118 | 85 | 56 | 0 | 14 | 98 | 29 | 113 | 4.05e-13 | 70.1 |
| MS.gene50176.t1 | MTR_3g098790 | 28.859 | 149 | 93 | 3 | 216 | 351 | 12 | 160 | 4.21e-13 | 70.1 |
| MS.gene50176.t1 | MTR_7g050580 | 30.357 | 112 | 75 | 2 | 14 | 124 | 29 | 138 | 5.05e-13 | 69.7 |
| MS.gene50176.t1 | MTR_7g050580 | 31.655 | 139 | 75 | 4 | 210 | 332 | 7 | 141 | 8.79e-13 | 68.9 |
| MS.gene50176.t1 | MTR_1g035460 | 34.579 | 107 | 64 | 2 | 14 | 120 | 2 | 102 | 1.85e-11 | 65.5 |
| MS.gene50176.t1 | MTR_1g035460 | 35.294 | 85 | 55 | 0 | 229 | 313 | 2 | 86 | 6.62e-11 | 63.9 |
| MS.gene50176.t1 | MTR_8g103940 | 32.143 | 84 | 57 | 0 | 13 | 96 | 54 | 137 | 4.20e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50176.t1 | AT3G18990 | 40.476 | 84 | 50 | 0 | 13 | 96 | 21 | 104 | 2.97e-17 | 82.8 |
| MS.gene50176.t1 | AT3G18990 | 39.394 | 99 | 56 | 1 | 217 | 311 | 6 | 104 | 5.45e-16 | 79.0 |
| MS.gene50176.t1 | AT3G18990 | 40.476 | 84 | 50 | 0 | 13 | 96 | 21 | 104 | 6.68e-17 | 82.0 |
| MS.gene50176.t1 | AT3G18990 | 39.394 | 99 | 56 | 1 | 217 | 311 | 6 | 104 | 1.06e-15 | 78.6 |
| MS.gene50176.t1 | AT1G49475 | 38.542 | 96 | 58 | 1 | 11 | 106 | 47 | 141 | 6.48e-16 | 75.9 |
| MS.gene50176.t1 | AT1G49475 | 35.398 | 113 | 64 | 2 | 215 | 321 | 32 | 141 | 9.53e-13 | 67.0 |
| MS.gene50176.t1 | AT4G01580 | 34.737 | 95 | 62 | 0 | 4 | 98 | 36 | 130 | 2.15e-15 | 74.7 |
| MS.gene50176.t1 | AT4G01580 | 33.058 | 121 | 75 | 2 | 202 | 316 | 13 | 133 | 7.36e-15 | 73.2 |
| MS.gene50176.t1 | AT3G18960 | 33.684 | 95 | 63 | 0 | 4 | 98 | 36 | 130 | 7.30e-15 | 73.2 |
| MS.gene50176.t1 | AT3G18960 | 31.933 | 119 | 75 | 2 | 201 | 313 | 12 | 130 | 1.05e-13 | 69.7 |
| MS.gene50176.t1 | AT3G18960 | 33.684 | 95 | 63 | 0 | 4 | 98 | 36 | 130 | 1.23e-14 | 72.8 |
| MS.gene50176.t1 | AT3G18960 | 31.148 | 122 | 78 | 2 | 201 | 316 | 12 | 133 | 9.92e-14 | 70.1 |
| MS.gene50176.t1 | AT3G18960 | 35.632 | 87 | 56 | 0 | 12 | 98 | 7 | 93 | 3.07e-14 | 70.9 |
| MS.gene50176.t1 | AT3G18960 | 31.461 | 89 | 61 | 0 | 228 | 316 | 8 | 96 | 4.05e-12 | 64.7 |
Find 77 sgRNAs with CRISPR-Local
Find 253 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGACTTTATAACAATATT+TGG | 0.103105 | 5.4:+62680357 | MS.gene50176:CDS |
| ACTGCTTTCCTTGAGAATTT+TGG | 0.150373 | 5.4:-62678146 | None:intergenic |
| TGTCTAAATATGCTTCTAAA+AGG | 0.186290 | 5.4:+62680709 | MS.gene50176:CDS |
| CTGTTCCGGATGGTCTAATT+TGG | 0.193330 | 5.4:+62680409 | MS.gene50176:CDS |
| GAGCACTTTGAACGAAAATA+AGG | 0.215729 | 5.4:-62680768 | None:intergenic |
| GGATGGTCTAATTTGGAAAA+TGG | 0.229629 | 5.4:+62680416 | MS.gene50176:CDS |
| GACGAGTTTATAACAAGATT+TGG | 0.231276 | 5.4:+62677668 | MS.gene50176:CDS |
| TATGGCCTTCTTAAAATGTT+TGG | 0.280661 | 5.4:-62680180 | None:intergenic |
| ATTTCCTATCCATTCAAAAC+TGG | 0.287220 | 5.4:+62677902 | MS.gene50176:CDS |
| CTGCAGTGACCAATCTTTAG+AGG | 0.292480 | 5.4:-62677636 | None:intergenic |
| GGACTATGACACTCTTTGTT+TGG | 0.315553 | 5.4:-62680630 | None:intergenic |
| GTTTATTGTGTATTGAATAA+AGG | 0.319096 | 5.4:+62678174 | MS.gene50176:CDS |
| TTCAAAACTCTAAGTACTAA+TGG | 0.332671 | 5.4:+62680603 | MS.gene50176:CDS |
| AAGTGAGAGTCAAGAGAAAA+AGG | 0.347170 | 5.4:+62680677 | MS.gene50176:CDS |
| AAGACTTTATAACAATATTT+GGG | 0.361172 | 5.4:+62680358 | MS.gene50176:CDS |
| ACGAGTTTATAACAAGATTT+GGG | 0.367534 | 5.4:+62677669 | MS.gene50176:CDS |
| TTCAAAACTGGGAAGACTAA+TGG | 0.374313 | 5.4:+62677914 | MS.gene50176:CDS |
| TGTTGCTACAATTACTGTTC+CGG | 0.380706 | 5.4:+62680395 | MS.gene50176:CDS |
| TGGAGCTTGAACAGAACATT+TGG | 0.381563 | 5.4:-62678126 | None:intergenic |
| CCTCACCACATATCTTCCTT+TGG | 0.412092 | 5.4:-62680790 | None:intergenic |
| ATGAATGGGTGACGGAAGTA+TGG | 0.414639 | 5.4:-62680198 | None:intergenic |
| TGCAGGCGGAGAAGGAAGTT+AGG | 0.415021 | 5.4:+62680153 | MS.gene50176:intron |
| AAAGCAATGCGAATAACGTT+TGG | 0.420748 | 5.4:+62678204 | MS.gene50176:CDS |
| ACTCTCACTTGTTTCAACCT+TGG | 0.424742 | 5.4:-62680664 | None:intergenic |
| TACTTGTCTTTCAAATATGA+AGG | 0.427472 | 5.4:+62680522 | MS.gene50176:CDS |
| GAACACTACTCTATCTGTTA+TGG | 0.428310 | 5.4:+62677806 | MS.gene50176:CDS |
| TATGTAGATTTCAAGTATGA+AGG | 0.431237 | 5.4:+62677833 | MS.gene50176:CDS |
| AATCTTGTTATAAACTCGTC+TGG | 0.437746 | 5.4:-62677665 | None:intergenic |
| TGACGGTTGCGAATGGCATA+TGG | 0.455390 | 5.4:+62677727 | MS.gene50176:CDS |
| AGCACTTTGAACGAAAATAA+GGG | 0.459706 | 5.4:-62680767 | None:intergenic |
| ATCACCCTTCCTCTAAAGAT+TGG | 0.475124 | 5.4:+62677627 | MS.gene50176:CDS |
| ACAAAGAGTGTCATAGTCCT+AGG | 0.483569 | 5.4:+62680634 | MS.gene50176:CDS |
| GTCTAAATATGCTTCTAAAA+GGG | 0.483677 | 5.4:+62680710 | MS.gene50176:CDS |
| TATTGAATTGCAGGCGGAGA+AGG | 0.498005 | 5.4:+62680145 | MS.gene50176:intron |
| ATATCGACAGTCAAAGGATT+GGG | 0.499850 | 5.4:-62677969 | None:intergenic |
| TTGTTTGGCATGACATGAAT+GGG | 0.500476 | 5.4:-62680212 | None:intergenic |
| TATTCTGCGGCGAATTCAGC+AGG | 0.501814 | 5.4:-62678096 | None:intergenic |
| ATATGCCATTCGCAACCGTC+AGG | 0.503250 | 5.4:-62677725 | None:intergenic |
| AAATACTATTCTGTAGGATA+CGG | 0.506013 | 5.4:+62680495 | MS.gene50176:CDS |
| TTTGTTTGGCATGACATGAA+TGG | 0.507824 | 5.4:-62680213 | None:intergenic |
| TTGGATTCTTTAGATTTGCA+AGG | 0.513403 | 5.4:+62678223 | MS.gene50176:CDS |
| AGTGTCATAGTCCTAGGAAA+AGG | 0.516090 | 5.4:+62680640 | MS.gene50176:CDS |
| CATATCGACAGTCAAAGGAT+TGG | 0.519552 | 5.4:-62677970 | None:intergenic |
| AAGGATTGGGAACGAAAAGA+AGG | 0.522583 | 5.4:-62677956 | None:intergenic |
| GCAGGCGGAGAAGGAAGTTA+GGG | 0.528822 | 5.4:+62680154 | MS.gene50176:intron |
| ACTTGTCTTTCAAATATGAA+GGG | 0.536470 | 5.4:+62680523 | MS.gene50176:CDS |
| CACCGCATATCGACAGTCAA+AGG | 0.544452 | 5.4:-62677975 | None:intergenic |
| TTTCCTATCCATTCAAAACT+GGG | 0.546790 | 5.4:+62677903 | MS.gene50176:CDS |
| TATTGTGTATTGAATAAAGG+TGG | 0.548350 | 5.4:+62678177 | MS.gene50176:CDS |
| AGGGAAAACAATCTATCAGA+TGG | 0.548736 | 5.4:+62678243 | MS.gene50176:CDS |
| ATGTAGATTTCAAGTATGAA+GGG | 0.551054 | 5.4:+62677834 | MS.gene50176:CDS |
| TTTCGTTCAAAGTGCTCCAA+AGG | 0.552472 | 5.4:+62680774 | MS.gene50176:CDS |
| TTCAAGCTCCAAAATTCTCA+AGG | 0.552648 | 5.4:+62678138 | MS.gene50176:CDS |
| ATATGCTTCTAAAAGGGCTG+AGG | 0.564166 | 5.4:+62680716 | MS.gene50176:CDS |
| TGGATTCTTTAGATTTGCAA+GGG | 0.565236 | 5.4:+62678224 | MS.gene50176:CDS |
| TGTGTATTGAATAAAGGTGG+CGG | 0.569727 | 5.4:+62678180 | MS.gene50176:CDS |
| CTGTTCCTGACGGTTGCGAA+TGG | 0.576577 | 5.4:+62677720 | MS.gene50176:CDS |
| AGTGACCAATCTTTAGAGGA+AGG | 0.582133 | 5.4:-62677632 | None:intergenic |
| AAAATTCTCAAGGAAAGCAG+TGG | 0.599450 | 5.4:+62678148 | MS.gene50176:CDS |
| ATAGAGTAGTGTTCTGCGAA+CGG | 0.599484 | 5.4:-62677797 | None:intergenic |
| AAAATGGAGTTGAAGATATG+TGG | 0.601427 | 5.4:+62680432 | MS.gene50176:CDS |
| AGGATTGGGAACGAAAAGAA+GGG | 0.605751 | 5.4:-62677955 | None:intergenic |
| GTGACCAATCTTTAGAGGAA+GGG | 0.612342 | 5.4:-62677631 | None:intergenic |
| GTGCTCCAAAGGAAGATATG+TGG | 0.623189 | 5.4:+62680785 | MS.gene50176:CDS |
| TAGTCCTAGGAAAAGGTCCA+AGG | 0.624175 | 5.4:+62680647 | MS.gene50176:CDS |
| GCTACGATTACTGTTCCTGA+CGG | 0.625910 | 5.4:+62677710 | MS.gene50176:CDS |
| ATTTGGCTTCAAATATTCTG+CGG | 0.631419 | 5.4:-62678109 | None:intergenic |
| ATCCTTTGACTGTCGATATG+CGG | 0.632326 | 5.4:+62677973 | MS.gene50176:CDS |
| AAATTCTCAAGGAAAGCAGT+GGG | 0.637805 | 5.4:+62678149 | MS.gene50176:CDS |
| GCTACAATTACTGTTCCGGA+TGG | 0.640460 | 5.4:+62680399 | MS.gene50176:CDS |
| GGAGTTGAAGATATGTGGCA+AGG | 0.642891 | 5.4:+62680437 | MS.gene50176:CDS |
| ACAAGTTACAGCTTCTCACA+CGG | 0.646742 | 5.4:+62678314 | MS.gene50176:CDS |
| ATGAGTTTGAGCTTATCAAG+AGG | 0.652349 | 5.4:+62678274 | MS.gene50176:CDS |
| GGCATGACATGAATGGGTGA+CGG | 0.656389 | 5.4:-62680206 | None:intergenic |
| CCAAAGGAAGATATGTGGTG+AGG | 0.663159 | 5.4:+62680790 | MS.gene50176:CDS |
| CATATGGAAGTGAAGAAATG+TGG | 0.695642 | 5.4:+62677743 | MS.gene50176:CDS |
| GTAACTTGTAACACAACACG+CGG | 0.711770 | 5.4:-62678300 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTTTTTGTATTTTTC+TGG | - | chr5.4:62679307-62679326 | None:intergenic | 10.0% |
| !!! | TTTTTTTTTTGTATTTTTCT+GGG | - | chr5.4:62679306-62679325 | None:intergenic | 10.0% |
| !! | AAGACTTTATAACAATATTT+GGG | + | chr5.4:62680358-62680377 | MS.gene50176:CDS | 15.0% |
| !! | AATATTAATGTATGAATGTA+CGG | - | chr5.4:62679867-62679886 | None:intergenic | 15.0% |
| !!! | AAAAATTCTTCTATTTTTGA+TGG | + | chr5.4:62679074-62679093 | MS.gene50176:intron | 15.0% |
| !!! | ATGTTTGAGTTATATATTTA+TGG | + | chr5.4:62678033-62678052 | MS.gene50176:intron | 15.0% |
| !! | ATAAAAAAGGAATATATTCC+CGG | + | chr5.4:62679437-62679456 | MS.gene50176:intron | 20.0% |
| !! | GAAGACTTTATAACAATATT+TGG | + | chr5.4:62680357-62680376 | MS.gene50176:CDS | 20.0% |
| !! | GTTTATTGTGTATTGAATAA+AGG | + | chr5.4:62678174-62678193 | MS.gene50176:CDS | 20.0% |
| !! | TGTTGTATTGTAATTCATAT+AGG | + | chr5.4:62678408-62678427 | MS.gene50176:intron | 20.0% |
| !! | TTGGAAAATGGTAAATATAT+GGG | - | chr5.4:62678951-62678970 | None:intergenic | 20.0% |
| !! | TTTGGAAAATGGTAAATATA+TGG | - | chr5.4:62678952-62678971 | None:intergenic | 20.0% |
| !!! | AAAAATTAGGAGCATAAAAA+AGG | + | chr5.4:62679424-62679443 | MS.gene50176:intron | 20.0% |
| !!! | AAGTTTTCTTTTGCAAAAAT+TGG | + | chr5.4:62677771-62677790 | MS.gene50176:CDS | 20.0% |
| !!! | ATTGATGATAGCTATAAAAA+GGG | - | chr5.4:62678646-62678665 | None:intergenic | 20.0% |
| !!! | GGATTTTACTATACATTATT+TGG | + | chr5.4:62677999-62678018 | MS.gene50176:intron | 20.0% |
| !!! | TTGATGATAATATAACTCAT+CGG | - | chr5.4:62680304-62680323 | None:intergenic | 20.0% |
| !!! | TTTTCAAAATACTATTCTGT+AGG | + | chr5.4:62680489-62680508 | MS.gene50176:CDS | 20.0% |
| ! | AAAATGGTAAATATATGGGA+GGG | - | chr5.4:62678947-62678966 | None:intergenic | 25.0% |
| ! | AAATACTATTCTGTAGGATA+CGG | + | chr5.4:62680495-62680514 | MS.gene50176:CDS | 25.0% |
| ! | ACGAGTTTATAACAAGATTT+GGG | + | chr5.4:62677669-62677688 | MS.gene50176:CDS | 25.0% |
| ! | ACTTGTCTTTCAAATATGAA+GGG | + | chr5.4:62680523-62680542 | MS.gene50176:CDS | 25.0% |
| ! | ATGAAAACAACTTTACCTTT+CGG | - | chr5.4:62678485-62678504 | None:intergenic | 25.0% |
| ! | CTTATTACTTATTGAATTGC+AGG | + | chr5.4:62680136-62680155 | MS.gene50176:intron | 25.0% |
| ! | GAATGAGAATTCCAAATAAA+TGG | - | chr5.4:62679043-62679062 | None:intergenic | 25.0% |
| ! | TAAATGGATGTATGAATGTA+TGG | - | chr5.4:62679839-62679858 | None:intergenic | 25.0% |
| ! | TACATACAATACACCAAAAA+TGG | + | chr5.4:62678568-62678587 | MS.gene50176:intron | 25.0% |
| ! | TACTTGTCTTTCAAATATGA+AGG | + | chr5.4:62680522-62680541 | MS.gene50176:CDS | 25.0% |
| ! | TATTGTGTATTGAATAAAGG+TGG | + | chr5.4:62678177-62678196 | MS.gene50176:CDS | 25.0% |
| ! | TGCTTGAAAAATTTGGAAAA+TGG | - | chr5.4:62678963-62678982 | None:intergenic | 25.0% |
| ! | TGTCTAAATATGCTTCTAAA+AGG | + | chr5.4:62680709-62680728 | MS.gene50176:CDS | 25.0% |
| ! | TTCAAAACTCTAAGTACTAA+TGG | + | chr5.4:62680603-62680622 | MS.gene50176:CDS | 25.0% |
| !! | ATGTAGATTTCAAGTATGAA+GGG | + | chr5.4:62677834-62677853 | MS.gene50176:CDS | 25.0% |
| !! | CATTGATGATAGCTATAAAA+AGG | - | chr5.4:62678647-62678666 | None:intergenic | 25.0% |
| !! | GGATTTTACTTTGTAGTTTT+TGG | + | chr5.4:62680811-62680830 | MS.gene50176:CDS | 25.0% |
| !! | GGTGAAATTTACAATTTGTT+TGG | - | chr5.4:62680230-62680249 | None:intergenic | 25.0% |
| !! | GTCTAAATATGCTTCTAAAA+GGG | + | chr5.4:62680710-62680729 | MS.gene50176:CDS | 25.0% |
| !! | TATGTAGATTTCAAGTATGA+AGG | + | chr5.4:62677833-62677852 | MS.gene50176:CDS | 25.0% |
| !!! | AACGAAAATAAGGGTTTTTT+GGG | - | chr5.4:62680761-62680780 | None:intergenic | 25.0% |
| !!! | ATTCATATAGGTAGTTTTGT+TGG | + | chr5.4:62678420-62678439 | MS.gene50176:intron | 25.0% |
| !!! | TTAGTACTTAGAGTTTTGAA+TGG | - | chr5.4:62680603-62680622 | None:intergenic | 25.0% |
| AAAATGGAGTTGAAGATATG+TGG | + | chr5.4:62680432-62680451 | MS.gene50176:CDS | 30.0% | |
| AAATTTGATGAATTGAGTGG+TGG | - | chr5.4:62679011-62679030 | None:intergenic | 30.0% | |
| AAGAATAATGAACTCGTATG+TGG | - | chr5.4:62680071-62680090 | None:intergenic | 30.0% | |
| AATGGAGGAACAATCTATTT+AGG | - | chr5.4:62678064-62678083 | None:intergenic | 30.0% | |
| AGCACTTTGAACGAAAATAA+GGG | - | chr5.4:62680770-62680789 | None:intergenic | 30.0% | |
| ATACAATACACCAAAAATGG+TGG | + | chr5.4:62678571-62678590 | MS.gene50176:intron | 30.0% | |
| ATTACTTATTGAATTGCAGG+CGG | + | chr5.4:62680139-62680158 | MS.gene50176:intron | 30.0% | |
| ATTTCCTATCCATTCAAAAC+TGG | + | chr5.4:62677902-62677921 | MS.gene50176:CDS | 30.0% | |
| CTCAAATTTGATGAATTGAG+TGG | - | chr5.4:62679014-62679033 | None:intergenic | 30.0% | |
| GAAAATGGTAAATATATGGG+AGG | - | chr5.4:62678948-62678967 | None:intergenic | 30.0% | |
| GACGAGTTTATAACAAGATT+TGG | + | chr5.4:62677668-62677687 | MS.gene50176:CDS | 30.0% | |
| TACAATACACCAAAAATGGT+GGG | + | chr5.4:62678572-62678591 | MS.gene50176:intron | 30.0% | |
| TATGGCCTTCTTAAAATGTT+TGG | - | chr5.4:62680183-62680202 | None:intergenic | 30.0% | |
| TCTTATGTAAAAACAGGGTA+AGG | + | chr5.4:62678544-62678563 | MS.gene50176:intron | 30.0% | |
| TGAATGTACGGTTGTATAAA+TGG | - | chr5.4:62679855-62679874 | None:intergenic | 30.0% | |
| TGATAGATTGAAACCCAAAA+GGG | + | chr5.4:62678749-62678768 | MS.gene50176:intron | 30.0% | |
| TGGATTCTTTAGATTTGCAA+GGG | + | chr5.4:62678224-62678243 | MS.gene50176:CDS | 30.0% | |
| TTGGATTCTTTAGATTTGCA+AGG | + | chr5.4:62678223-62678242 | MS.gene50176:CDS | 30.0% | |
| TTTCCTATCCATTCAAAACT+GGG | + | chr5.4:62677903-62677922 | MS.gene50176:CDS | 30.0% | |
| ! | AATCTTGTTATAAACTCGTC+TGG | - | chr5.4:62677668-62677687 | None:intergenic | 30.0% |
| ! | ACGTTTTCTTTTGCAACAAA+TGG | + | chr5.4:62680460-62680479 | MS.gene50176:CDS | 30.0% |
| ! | ATTTGGCTTCAAATATTCTG+CGG | - | chr5.4:62678112-62678131 | None:intergenic | 30.0% |
| ! | ATTTTCCAAATTAGACCATC+CGG | - | chr5.4:62680417-62680436 | None:intergenic | 30.0% |
| !! | CAGACTTTTGGTTAAACAAT+GGG | + | chr5.4:62679603-62679622 | MS.gene50176:intron | 30.0% |
| !! | GGAATCCAAACATTTTAAGA+AGG | + | chr5.4:62680175-62680194 | MS.gene50176:CDS | 30.0% |
| !!! | AACGAAAAGAAGGGTTTTTT+GGG | - | chr5.4:62677949-62677968 | None:intergenic | 30.0% |
| !!! | GAACGAAAATAAGGGTTTTT+TGG | - | chr5.4:62680762-62680781 | None:intergenic | 30.0% |
| !!! | TTACCCTGTTTTTACATAAG+AGG | - | chr5.4:62678545-62678564 | None:intergenic | 30.0% |
| AAAAAGGAATATATTCCCGG+TGG | + | chr5.4:62679440-62679459 | MS.gene50176:intron | 35.0% | |
| AAAATTCTCAAGGAAAGCAG+TGG | + | chr5.4:62678148-62678167 | MS.gene50176:CDS | 35.0% | |
| AAAGCAATGCGAATAACGTT+TGG | + | chr5.4:62678204-62678223 | MS.gene50176:CDS | 35.0% | |
| AAATTCTCAAGGAAAGCAGT+GGG | + | chr5.4:62678149-62678168 | MS.gene50176:CDS | 35.0% | |
| AAGTGAGAGTCAAGAGAAAA+AGG | + | chr5.4:62680677-62680696 | MS.gene50176:CDS | 35.0% | |
| ACAGCCTCTTATGTAAAAAC+AGG | + | chr5.4:62678538-62678557 | MS.gene50176:intron | 35.0% | |
| AGGGAAAACAATCTATCAGA+TGG | + | chr5.4:62678243-62678262 | MS.gene50176:CDS | 35.0% | |
| AGTTATCATCAATGACCGAA+AGG | + | chr5.4:62678467-62678486 | MS.gene50176:intron | 35.0% | |
| ATACGACCAAACAAGACTTT+CGG | - | chr5.4:62679960-62679979 | None:intergenic | 35.0% | |
| ATATCGACAGTCAAAGGATT+GGG | - | chr5.4:62677972-62677991 | None:intergenic | 35.0% | |
| ATGAGTTTGAGCTTATCAAG+AGG | + | chr5.4:62678274-62678293 | MS.gene50176:CDS | 35.0% | |
| CAGATAATGAGAAAAGAGTG+AGG | - | chr5.4:62679983-62680002 | None:intergenic | 35.0% | |
| CAGCCTCTTATGTAAAAACA+GGG | + | chr5.4:62678539-62678558 | MS.gene50176:intron | 35.0% | |
| CATATGGAAGTGAAGAAATG+TGG | + | chr5.4:62677743-62677762 | MS.gene50176:CDS | 35.0% | |
| CCATTGTTTAACCAAAAGTC+TGG | - | chr5.4:62679605-62679624 | None:intergenic | 35.0% | |
| CTGATAGATTGAAACCCAAA+AGG | + | chr5.4:62678748-62678767 | MS.gene50176:intron | 35.0% | |
| GAACACTACTCTATCTGTTA+TGG | + | chr5.4:62677806-62677825 | MS.gene50176:CDS | 35.0% | |
| GAGCACTTTGAACGAAAATA+AGG | - | chr5.4:62680771-62680790 | None:intergenic | 35.0% | |
| GCTATCATCAATGAGCAATA+TGG | + | chr5.4:62678654-62678673 | MS.gene50176:intron | 35.0% | |
| GGATGGTCTAATTTGGAAAA+TGG | + | chr5.4:62680416-62680435 | MS.gene50176:CDS | 35.0% | |
| TACACAACATCATCCATACA+TGG | + | chr5.4:62679922-62679941 | MS.gene50176:intron | 35.0% | |
| TAGCTATAAAAAGGGCAATC+CGG | - | chr5.4:62678638-62678657 | None:intergenic | 35.0% | |
| TCACAATGATATCATCTCCA+AGG | + | chr5.4:62679694-62679713 | MS.gene50176:intron | 35.0% | |
| TGTGTATTGAATAAAGGTGG+CGG | + | chr5.4:62678180-62678199 | MS.gene50176:CDS | 35.0% | |
| TGTTGCTACAATTACTGTTC+CGG | + | chr5.4:62680395-62680414 | MS.gene50176:CDS | 35.0% | |
| TTCAAAACTGGGAAGACTAA+TGG | + | chr5.4:62677914-62677933 | MS.gene50176:CDS | 35.0% | |
| TTCAAGCTCCAAAATTCTCA+AGG | + | chr5.4:62678138-62678157 | MS.gene50176:CDS | 35.0% | |
| TTGTTTGGCATGACATGAAT+GGG | - | chr5.4:62680215-62680234 | None:intergenic | 35.0% | |
| TTTGTTTGGCATGACATGAA+TGG | - | chr5.4:62680216-62680235 | None:intergenic | 35.0% | |
| ! | AAAGTTGTCAGGGAGTATAA+CGG | + | chr5.4:62678904-62678923 | MS.gene50176:intron | 35.0% |
| ! | ACTGCTTTCCTTGAGAATTT+TGG | - | chr5.4:62678149-62678168 | None:intergenic | 35.0% |
| ! | TCATGTCTCTAATTTTTGCC+TGG | - | chr5.4:62678713-62678732 | None:intergenic | 35.0% |
| ! | TCTAAGTTGAAAACCCGAAA+GGG | + | chr5.4:62679343-62679362 | MS.gene50176:intron | 35.0% |
| ! | TTATGCTCCTAATTTTTGCC+TGG | - | chr5.4:62679421-62679440 | None:intergenic | 35.0% |
| !! | CCAGACTTTTGGTTAAACAA+TGG | + | chr5.4:62679602-62679621 | MS.gene50176:intron | 35.0% |
| !! | GTTGTTTTCATGTGACTGAA+AGG | + | chr5.4:62678494-62678513 | MS.gene50176:intron | 35.0% |
| !! | TAACTCATCGGTTCTACTAT+AGG | - | chr5.4:62680292-62680311 | None:intergenic | 35.0% |
| !! | TTGAGCATGTGCCATTTATT+TGG | + | chr5.4:62679029-62679048 | MS.gene50176:intron | 35.0% |
| !!! | AAAGAAGGGTTTTTTGGGTT+TGG | - | chr5.4:62677944-62677963 | None:intergenic | 35.0% |
| !!! | AGGTAGTTTTGTTGGTTGTT+AGG | + | chr5.4:62678428-62678447 | MS.gene50176:intron | 35.0% |
| !!! | CTTTTGGGTTTCAATCTATC+AGG | - | chr5.4:62678750-62678769 | None:intergenic | 35.0% |
| !!! | GAACGAAAAGAAGGGTTTTT+TGG | - | chr5.4:62677950-62677969 | None:intergenic | 35.0% |
| !!! | TTAGTCTTCCCAGTTTTGAA+TGG | - | chr5.4:62677914-62677933 | None:intergenic | 35.0% |
| AACAAATTGAGCCGTTGATG+GGG | - | chr5.4:62679675-62679694 | None:intergenic | 40.0% | |
| AAGGATTGGGAACGAAAAGA+AGG | - | chr5.4:62677959-62677978 | None:intergenic | 40.0% | |
| ACAAGTTACAGCTTCTCACA+CGG | + | chr5.4:62678314-62678333 | MS.gene50176:CDS | 40.0% | |
| ACTCTCACTTGTTTCAACCT+TGG | - | chr5.4:62680667-62680686 | None:intergenic | 40.0% | |
| AGAAACATGGCTAAGCACTA+TGG | + | chr5.4:62679245-62679264 | MS.gene50176:intron | 40.0% | |
| AGCAGGAACATACTACACAA+TGG | - | chr5.4:62678082-62678101 | None:intergenic | 40.0% | |
| AGGAACATACTACACAATGG+AGG | - | chr5.4:62678079-62678098 | None:intergenic | 40.0% | |
| AGGATTGGGAACGAAAAGAA+GGG | - | chr5.4:62677958-62677977 | None:intergenic | 40.0% | |
| AGTGTCATAGTCCTAGGAAA+AGG | + | chr5.4:62680640-62680659 | MS.gene50176:CDS | 40.0% | |
| ATAGAGTAGTGTTCTGCGAA+CGG | - | chr5.4:62677800-62677819 | None:intergenic | 40.0% | |
| ATCCTTTGACTGTCGATATG+CGG | + | chr5.4:62677973-62677992 | MS.gene50176:CDS | 40.0% | |
| ATGATTGTTGAAGCATGCCA+GGG | + | chr5.4:62679540-62679559 | MS.gene50176:intron | 40.0% | |
| CATATCGACAGTCAAAGGAT+TGG | - | chr5.4:62677973-62677992 | None:intergenic | 40.0% | |
| CCACGGATGCTTGAAAAATT+TGG | - | chr5.4:62678970-62678989 | None:intergenic | 40.0% | |
| GAACAAATTGAGCCGTTGAT+GGG | - | chr5.4:62679676-62679695 | None:intergenic | 40.0% | |
| GCTTAGCCATGTTTCTCTTT+CGG | - | chr5.4:62679241-62679260 | None:intergenic | 40.0% | |
| GGACTATGACACTCTTTGTT+TGG | - | chr5.4:62680633-62680652 | None:intergenic | 40.0% | |
| GGCGATCTATGCAAAAGTTA+AGG | + | chr5.4:62678770-62678789 | MS.gene50176:intron | 40.0% | |
| TACACTCCGAAAGAGAAACA+TGG | + | chr5.4:62679232-62679251 | MS.gene50176:intron | 40.0% | |
| TCATCCATACATGGTTGCAT+CGG | + | chr5.4:62679931-62679950 | MS.gene50176:intron | 40.0% | |
| TGAACAAATTGAGCCGTTGA+TGG | - | chr5.4:62679677-62679696 | None:intergenic | 40.0% | |
| TGGAGCTTGAACAGAACATT+TGG | - | chr5.4:62678129-62678148 | None:intergenic | 40.0% | |
| TGTAGACTGAAAACCCGAAA+GGG | + | chr5.4:62678683-62678702 | MS.gene50176:intron | 40.0% | |
| TGTATGAAACGAGACAAGAC+AGG | - | chr5.4:62679768-62679787 | None:intergenic | 40.0% | |
| TTCATGTGACTGAAAGGTCA+CGG | + | chr5.4:62678500-62678519 | MS.gene50176:intron | 40.0% | |
| ! | AGTGACCAATCTTTAGAGGA+AGG | - | chr5.4:62677635-62677654 | None:intergenic | 40.0% |
| ! | ATATGCTTCTAAAAGGGCTG+AGG | + | chr5.4:62680716-62680735 | MS.gene50176:CDS | 40.0% |
| ! | ATCACCCTTCCTCTAAAGAT+TGG | + | chr5.4:62677627-62677646 | MS.gene50176:CDS | 40.0% |
| ! | CTCTAAGTTGAAAACCCGAA+AGG | + | chr5.4:62679342-62679361 | MS.gene50176:intron | 40.0% |
| ! | GAAAATCCCTAACTTTTGCC+TGG | - | chr5.4:62679488-62679507 | None:intergenic | 40.0% |
| ! | GTAACTTGTAACACAACACG+CGG | - | chr5.4:62678303-62678322 | None:intergenic | 40.0% |
| ! | GTGACCAATCTTTAGAGGAA+GGG | - | chr5.4:62677634-62677653 | None:intergenic | 40.0% |
| ! | TTACATAAGAGGCTGTTTCC+AGG | - | chr5.4:62678534-62678553 | None:intergenic | 40.0% |
| ! | TTTCGTTCAAAGTGCTCCAA+AGG | + | chr5.4:62680774-62680793 | MS.gene50176:CDS | 40.0% |
| ! | TTTGCATAGATCGCCCTTTT+GGG | - | chr5.4:62678765-62678784 | None:intergenic | 40.0% |
| ! | TTTTGCATAGATCGCCCTTT+TGG | - | chr5.4:62678766-62678785 | None:intergenic | 40.0% |
| !! | AACAATGGGTTTTGCTCTCT+AGG | + | chr5.4:62679617-62679636 | MS.gene50176:intron | 40.0% |
| !! | ACAAAGAGTGTCATAGTCCT+AGG | + | chr5.4:62680634-62680653 | MS.gene50176:CDS | 40.0% |
| !!! | CCAAATTTTTCAAGCATCCG+TGG | + | chr5.4:62678967-62678986 | MS.gene50176:intron | 40.0% |
| !!! | CTTCCCAGTTTTGAATGGAT+AGG | - | chr5.4:62677909-62677928 | None:intergenic | 40.0% |
| !!! | GTTTTTTGGGCTGAATGCAT+TGG | - | chr5.4:62680748-62680767 | None:intergenic | 40.0% |
| AAACACTCGGCAAACTTCCT+TGG | - | chr5.4:62679714-62679733 | None:intergenic | 45.0% | |
| AACTACCCACGAGTTCAAAG+AGG | - | chr5.4:62679179-62679198 | None:intergenic | 45.0% | |
| AAGTTGAAAACCCGAAAGGG+CGG | + | chr5.4:62679346-62679365 | MS.gene50176:intron | 45.0% | |
| ACGAGACAAGACAGGTAAGA+TGG | - | chr5.4:62679760-62679779 | None:intergenic | 45.0% | |
| ACTACCCACGAGTTCAAAGA+GGG | - | chr5.4:62679178-62679197 | None:intergenic | 45.0% | |
| AGATCAAAGACTCGGAACTG+GGG | + | chr5.4:62678876-62678895 | MS.gene50176:intron | 45.0% | |
| ATCAGCCAACACAGTCCTTT+CGG | + | chr5.4:62678811-62678830 | MS.gene50176:intron | 45.0% | |
| CAGATCAAAGACTCGGAACT+GGG | + | chr5.4:62678875-62678894 | MS.gene50176:intron | 45.0% | |
| CATGATTGTTGAAGCATGCC+AGG | + | chr5.4:62679539-62679558 | MS.gene50176:intron | 45.0% | |
| CCAAAGGAAGATATGTGGTG+AGG | + | chr5.4:62680790-62680809 | MS.gene50176:CDS | 45.0% | |
| CCTCACCACATATCTTCCTT+TGG | - | chr5.4:62680793-62680812 | None:intergenic | 45.0% | |
| CGAGACAAGACAGGTAAGAT+GGG | - | chr5.4:62679759-62679778 | None:intergenic | 45.0% | |
| CTAAAACTCCCGCATACTCA+AGG | - | chr5.4:62678611-62678630 | None:intergenic | 45.0% | |
| CTGCAGTGACCAATCTTTAG+AGG | - | chr5.4:62677639-62677658 | None:intergenic | 45.0% | |
| CTGTTCCGGATGGTCTAATT+TGG | + | chr5.4:62680409-62680428 | MS.gene50176:CDS | 45.0% | |
| GACATCGCAGTACAAACACT+CGG | - | chr5.4:62679727-62679746 | None:intergenic | 45.0% | |
| GATCTGCAGATGCTTCGAAT+AGG | - | chr5.4:62678861-62678880 | None:intergenic | 45.0% | |
| GCATCTGCAGATCAAAGACT+CGG | + | chr5.4:62678868-62678887 | MS.gene50176:intron | 45.0% | |
| GCTACAATTACTGTTCCGGA+TGG | + | chr5.4:62680399-62680418 | MS.gene50176:CDS | 45.0% | |
| GCTACGATTACTGTTCCTGA+CGG | + | chr5.4:62677710-62677729 | MS.gene50176:CDS | 45.0% | |
| GGAGTTGAAGATATGTGGCA+AGG | + | chr5.4:62680437-62680456 | MS.gene50176:CDS | 45.0% | |
| GTGCTCCAAAGGAAGATATG+TGG | + | chr5.4:62680785-62680804 | MS.gene50176:CDS | 45.0% | |
| GTGTAGACTGAAAACCCGAA+AGG | + | chr5.4:62678682-62678701 | MS.gene50176:intron | 45.0% | |
| TAGTCCTAGGAAAAGGTCCA+AGG | + | chr5.4:62680647-62680666 | MS.gene50176:CDS | 45.0% | |
| TATTGAATTGCAGGCGGAGA+AGG | + | chr5.4:62680145-62680164 | MS.gene50176:intron | 45.0% | |
| TCATGTGACTGAAAGGTCAC+GGG | + | chr5.4:62678501-62678520 | MS.gene50176:intron | 45.0% | |
| TCGCCATTTGTAAATCAGCG+CGG | - | chr5.4:62679576-62679595 | None:intergenic | 45.0% | |
| TGATTGTTGAAGCATGCCAG+GGG | + | chr5.4:62679541-62679560 | MS.gene50176:intron | 45.0% | |
| ! | TAGGAGGTTCTTTTGACGAC+TGG | - | chr5.4:62678842-62678861 | None:intergenic | 45.0% |
| ! | TCAACCTTGGACCTTTTCCT+AGG | - | chr5.4:62680654-62680673 | None:intergenic | 45.0% |
| ! | TGGGGCATTCAAAGTTGTCA+GGG | + | chr5.4:62678894-62678913 | MS.gene50176:intron | 45.0% |
| !! | ACTCCGCGCTGATTTACAAA+TGG | + | chr5.4:62679570-62679589 | MS.gene50176:intron | 45.0% |
| !! | ATGAATGGGTGACGGAAGTA+TGG | - | chr5.4:62680201-62680220 | None:intergenic | 45.0% |
| AAGACAGGTAAGATGGGAGG+TGG | - | chr5.4:62679753-62679772 | None:intergenic | 50.0% | |
| ACACACCCTCTTTGAACTCG+TGG | + | chr5.4:62679170-62679189 | MS.gene50176:intron | 50.0% | |
| AGACTGAAAACCCGAAAGGG+CGG | + | chr5.4:62678686-62678705 | MS.gene50176:intron | 50.0% | |
| AGAGAGAGAGAGAGAGAGAG+AGG | - | chr5.4:62680249-62680268 | None:intergenic | 50.0% | |
| ATATGCCATTCGCAACCGTC+AGG | - | chr5.4:62677728-62677747 | None:intergenic | 50.0% | |
| ATCAGCATGCGCCTTGAGAA+CGG | + | chr5.4:62679208-62679227 | MS.gene50176:intron | 50.0% | |
| ATCAGTTGCTACCTTGTGCC+GGG | + | chr5.4:62679644-62679663 | MS.gene50176:intron | 50.0% | |
| ATTGAGTGGTGGCCTACCAA+TGG | - | chr5.4:62679000-62679019 | None:intergenic | 50.0% | |
| CACACCCTCTTTGAACTCGT+GGG | + | chr5.4:62679171-62679190 | MS.gene50176:intron | 50.0% | |
| CACCGCATATCGACAGTCAA+AGG | - | chr5.4:62677978-62677997 | None:intergenic | 50.0% | |
| CATCAGTTGCTACCTTGTGC+CGG | + | chr5.4:62679643-62679662 | MS.gene50176:intron | 50.0% | |
| CATGGCTAAGCACTATGGAG+AGG | + | chr5.4:62679250-62679269 | MS.gene50176:intron | 50.0% | |
| CTGCAGATGCTTCGAATAGG+AGG | - | chr5.4:62678858-62678877 | None:intergenic | 50.0% | |
| GACAAGACAGGTAAGATGGG+AGG | - | chr5.4:62679756-62679775 | None:intergenic | 50.0% | |
| GCAGATCAAAGACTCGGAAC+TGG | + | chr5.4:62678874-62678893 | MS.gene50176:intron | 50.0% | |
| GGCATGACATGAATGGGTGA+CGG | - | chr5.4:62680209-62680228 | None:intergenic | 50.0% | |
| TATTCTGCGGCGAATTCAGC+AGG | - | chr5.4:62678099-62678118 | None:intergenic | 50.0% | |
| TATTGCCTAAGCCGCCCTTT+CGG | - | chr5.4:62679360-62679379 | None:intergenic | 50.0% | |
| TCAGCCAACACAGTCCTTTC+GGG | + | chr5.4:62678812-62678831 | MS.gene50176:intron | 50.0% | |
| TCGGAGTGTAACCGTTCTCA+AGG | - | chr5.4:62679222-62679241 | None:intergenic | 50.0% | |
| TGACGGTTGCGAATGGCATA+TGG | + | chr5.4:62677727-62677746 | MS.gene50176:CDS | 50.0% | |
| TTGACTGTCGATATGCGGTG+AGG | + | chr5.4:62677978-62677997 | MS.gene50176:intron | 50.0% | |
| ! | CTGGGGCATTCAAAGTTGTC+AGG | + | chr5.4:62678893-62678912 | MS.gene50176:intron | 50.0% |
| ! | GGCGACTTCTTCCAGACTTT+TGG | + | chr5.4:62679591-62679610 | MS.gene50176:intron | 50.0% |
| !! | ATGCGGGAGTTTTAGTGCAC+CGG | + | chr5.4:62678616-62678635 | MS.gene50176:intron | 50.0% |
| !! | GGTGGACTGAAAACCCGAAA+GGG | + | chr5.4:62679391-62679410 | MS.gene50176:intron | 50.0% |
| !! | TTAGGCAATAGAGAGCGTCC+CGG | + | chr5.4:62679370-62679389 | MS.gene50176:intron | 50.0% |
| !!! | CTTTCGGGTTTTCAGTCCAC+CGG | - | chr5.4:62679392-62679411 | None:intergenic | 50.0% |
| !!! | TTTCGGGTTTTCAGTCCACC+GGG | - | chr5.4:62679391-62679410 | None:intergenic | 50.0% |
| AAAACCCGAAAGGGCGGCTT+AGG | + | chr5.4:62679352-62679371 | MS.gene50176:intron | 55.0% | |
| AAGGTCACGGGTTCAAGTCC+TGG | + | chr5.4:62678513-62678532 | MS.gene50176:intron | 55.0% | |
| ACTCCCGCATACTCAAGGTC+CGG | - | chr5.4:62678606-62678625 | None:intergenic | 55.0% | |
| ATGCCCCGAAAGGACTGTGT+TGG | - | chr5.4:62678819-62678838 | None:intergenic | 55.0% | |
| ATTGCCTAAGCCGCCCTTTC+GGG | - | chr5.4:62679359-62679378 | None:intergenic | 55.0% | |
| CAGCCAACACAGTCCTTTCG+GGG | + | chr5.4:62678813-62678832 | MS.gene50176:intron | 55.0% | |
| CATACTCAAGGTCCGGGAAG+GGG | - | chr5.4:62678599-62678618 | None:intergenic | 55.0% | |
| CTACCAATGGCATGGCTCCA+CGG | - | chr5.4:62678987-62679006 | None:intergenic | 55.0% | |
| CTGTTCCTGACGGTTGCGAA+TGG | + | chr5.4:62677720-62677739 | MS.gene50176:CDS | 55.0% | |
| CTTCCCGGACCTTGAGTATG+CGG | + | chr5.4:62678599-62678618 | MS.gene50176:intron | 55.0% | |
| GCAGGCGGAGAAGGAAGTTA+GGG | + | chr5.4:62680154-62680173 | MS.gene50176:intron | 55.0% | |
| GCATACTCAAGGTCCGGGAA+GGG | - | chr5.4:62678600-62678619 | None:intergenic | 55.0% | |
| GCGACCGATGCAACCATGTA+TGG | - | chr5.4:62679938-62679957 | None:intergenic | 55.0% | |
| GGACTGAAAACCCGAAAGGG+CGG | + | chr5.4:62679394-62679413 | MS.gene50176:intron | 55.0% | |
| GGCGGTCCAGGCAAAAGTTA+GGG | + | chr5.4:62679479-62679498 | MS.gene50176:intron | 55.0% | |
| GGGCGGTCCAGGCAAAAATT+AGG | + | chr5.4:62678703-62678722 | MS.gene50176:intron | 55.0% | |
| TATGTGGCGTGACGTGTGAG+CGG | - | chr5.4:62680055-62680074 | None:intergenic | 55.0% | |
| TGCAGGCGGAGAAGGAAGTT+AGG | + | chr5.4:62680153-62680172 | MS.gene50176:intron | 55.0% | |
| TTCCCGGACCTTGAGTATGC+GGG | + | chr5.4:62678600-62678619 | MS.gene50176:intron | 55.0% | |
| ! | AAAATGGTGGGACCCCTTCC+CGG | + | chr5.4:62678584-62678603 | MS.gene50176:intron | 55.0% |
| ! | GGAAGGGGTCCCACCATTTT+TGG | - | chr5.4:62678584-62678603 | None:intergenic | 55.0% |
| ! | TTTTGCCTGGACCGCCCTTT+CGG | - | chr5.4:62678700-62678719 | None:intergenic | 55.0% |
| !! | CGGTCGCCGAAAGTCTTGTT+TGG | + | chr5.4:62679951-62679970 | MS.gene50176:intron | 55.0% |
| !! | CGGTGGACTGAAAACCCGAA+AGG | + | chr5.4:62679390-62679409 | MS.gene50176:intron | 55.0% |
| AAAACCCGAAAGGGCGGTCC+AGG | + | chr5.4:62678692-62678711 | MS.gene50176:intron | 60.0% | |
| CATCCGTGGAGCCATGCCAT+TGG | + | chr5.4:62678981-62679000 | MS.gene50176:intron | 60.0% | |
| CGCATACTCAAGGTCCGGGA+AGG | - | chr5.4:62678601-62678620 | None:intergenic | 60.0% | |
| CGTGGAGCCATGCCATTGGT+AGG | + | chr5.4:62678985-62679004 | MS.gene50176:intron | 60.0% | |
| CTCCCGCATACTCAAGGTCC+GGG | - | chr5.4:62678605-62678624 | None:intergenic | 60.0% | |
| GGGCGGTCCAGGCAAAAGTT+AGG | + | chr5.4:62679478-62679497 | MS.gene50176:intron | 60.0% | |
| GTGGTGGCCTACCAATGGCA+TGG | - | chr5.4:62678995-62679014 | None:intergenic | 60.0% | |
| TGACGACTGGATGCCCCGAA+AGG | - | chr5.4:62678829-62678848 | None:intergenic | 60.0% | |
| TTTGCCTGGACCGCCCTTTC+GGG | - | chr5.4:62678699-62678718 | None:intergenic | 60.0% | |
| ATGGGGTGAAGCCCGGCACA+AGG | - | chr5.4:62679658-62679677 | None:intergenic | 65.0% | |
| GCCGGGCTTCACCCCATCAA+CGG | + | chr5.4:62679661-62679680 | MS.gene50176:intron | 65.0% | |
| ! | GCCGTTGATGGGGTGAAGCC+CGG | - | chr5.4:62679665-62679684 | None:intergenic | 65.0% |
| !! | AGCGCGGAGTGCTTTTCCCC+TGG | - | chr5.4:62679560-62679579 | None:intergenic | 65.0% |
| !! | GGCAATAGAGAGCGTCCCGG+TGG | + | chr5.4:62679373-62679392 | MS.gene50176:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 62677623 | 62680866 | 62677623 | ID=MS.gene50176 |
| chr5.4 | mRNA | 62677623 | 62680866 | 62677623 | ID=MS.gene50176.t1;Parent=MS.gene50176 |
| chr5.4 | exon | 62677623 | 62677994 | 62677623 | ID=MS.gene50176.t1.exon1;Parent=MS.gene50176.t1 |
| chr5.4 | CDS | 62677623 | 62677994 | 62677623 | ID=cds.MS.gene50176.t1;Parent=MS.gene50176.t1 |
| chr5.4 | exon | 62678090 | 62678335 | 62678090 | ID=MS.gene50176.t1.exon2;Parent=MS.gene50176.t1 |
| chr5.4 | CDS | 62678090 | 62678335 | 62678090 | ID=cds.MS.gene50176.t1;Parent=MS.gene50176.t1 |
| chr5.4 | exon | 62680158 | 62680220 | 62680158 | ID=MS.gene50176.t1.exon3;Parent=MS.gene50176.t1 |
| chr5.4 | CDS | 62680158 | 62680220 | 62680158 | ID=cds.MS.gene50176.t1;Parent=MS.gene50176.t1 |
| chr5.4 | exon | 62680348 | 62680866 | 62680348 | ID=MS.gene50176.t1.exon4;Parent=MS.gene50176.t1 |
| chr5.4 | CDS | 62680348 | 62680866 | 62680348 | ID=cds.MS.gene50176.t1;Parent=MS.gene50176.t1 |
| Gene Sequence |
| Protein sequence |
