AlfalfaGEDB | MS.gene50181

M. sativa cultivar XinJiangDaYe / MS.gene50181

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF00628.32 PF05641.15 PF16135.8

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50181.t1	AES98667.1	92.4	1144	65	1	1	1122	1	1144	0.00E+00	2125.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50181.t1	F4IXE7	34.1	419	271	5	654	1071	618	1032	9.7e-73	276.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50181.t1	G7JZJ8	92.4	1144	65	1	1	1122	1	1144	0.0e+00	2125.5

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene50181.t1	TR	PHD

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50181.t1	MTR_5g071340	95.017	1144	35	1	1	1122	1	1144	0.0	2240
MS.gene50181.t1	MTR_3g464550	46.803	1126	495	20	21	1118	11	1060	0.0	969
MS.gene50181.t1	MTR_5g024390	43.155	431	206	9	657	1062	3	419	6.52e-112	355
MS.gene50181.t1	MTR_5g024500	42.691	431	208	9	657	1062	3	419	1.32e-110	352
MS.gene50181.t1	MTR_7g109640	44.578	415	212	8	656	1062	383	787	9.23e-110	362
MS.gene50181.t1	MTR_3g103120	40.042	472	246	10	615	1064	378	834	5.57e-101	339
MS.gene50181.t1	MTR_3g103110	35.928	501	245	9	626	1064	307	793	2.34e-99	333
MS.gene50181.t1	MTR_4g027030	54.941	253	103	5	6	253	3	249	3.08e-84	274
MS.gene50181.t1	MTR_2g078040	35.556	495	298	10	576	1062	685	1166	1.73e-82	295
MS.gene50181.t1	MTR_1g103830	34.791	503	295	10	568	1063	537	1013	9.10e-76	275

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50181.t1	AT5G58610	37.601	1117	594	29	12	1106	14	1049	0.0	671
MS.gene50181.t1	AT5G58610	38.326	1075	563	28	12	1064	14	1010	0.0	670
MS.gene50181.t1	AT5G58610	38.326	1075	563	28	12	1064	14	1010	0.0	670
MS.gene50181.t1	AT5G58610	37.557	1105	587	29	24	1106	17	1040	0.0	658
MS.gene50181.t1	AT5G58610	37.557	1105	587	29	24	1106	17	1040	0.0	657
MS.gene50181.t1	AT5G58610	36.742	1105	570	31	24	1106	17	1014	0.0	624
MS.gene50181.t1	AT5G58610	36.742	1105	570	31	24	1106	17	1014	0.0	623
MS.gene50181.t1	AT5G58610	37.120	1021	542	28	12	1010	14	956	0.0	600
MS.gene50181.t1	AT5G58610	37.066	1009	535	28	24	1010	17	947	0.0	587
MS.gene50181.t1	AT5G58610	36.148	971	520	28	24	972	17	909	2.23e-174	536

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Find 250 sgRNAs with CRISPR-Local

Find 444 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCGAAGTGTAAAACTT+TGG	0.406804	5.4:+62557140	None:intergenic
AAAATTGAAGAGACCGAGGA+TGG	0.598542	5.4:-62555863	MS.gene50181:CDS
AAACCTTGGCTCATTTGGGG+TGG	0.502467	5.4:+62557029	None:intergenic
AAATAAAATTGAAGAGACCG+AGG	0.667269	5.4:-62555867	MS.gene50181:CDS
AAATGAAGAATTGATTAGTG+TGG	0.598410	5.4:-62553982	MS.gene50181:CDS
AACAGATTTGGTATGATATA+AGG	0.374438	5.4:-62557844	MS.gene50181:CDS
AACATCTGTTAGCAGAAGGA+TGG	0.542192	5.4:-62556860	MS.gene50181:CDS
AACATCTTGTTTATCTTGGA+TGG	0.419268	5.4:-62557235	MS.gene50181:CDS
AACCAACAAGTTTGAGGAGT+CGG	0.543763	5.4:+62557565	None:intergenic
AACGCGTCTTCCTCCCACAG+CGG	0.747933	5.4:+62558296	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAAATCTAAGCACGGA+AGG	+	chr5.4:62557729-62557748	None:intergenic	30.0%
AAAAAACATCTGTTAGCAGA+AGG	-	chr5.4:62555164-62555183	MS.gene50181:intron	30.0%
AAACTAGAATAAAAAAGGGG+GGG	+	chr5.4:62556478-62556497	None:intergenic	30.0%
AAATAAAATTGAAGAGACCG+AGG	-	chr5.4:62556161-62556180	MS.gene50181:CDS	30.0%
AAGACGTTATATTCAACCAA+AGG	-	chr5.4:62557118-62557137	MS.gene50181:CDS	30.0%
AATCTGATTCTTCATCATCA+AGG	+	chr5.4:62553777-62553796	None:intergenic	30.0%
AATTACAATTCAAATGCCAC+AGG	+	chr5.4:62558359-62558378	None:intergenic	30.0%
ACTTGAAATTTAACTGGAGA+AGG	+	chr5.4:62555050-62555069	None:intergenic	30.0%
AGATTATCTTATTGAAGTGG+TGG	-	chr5.4:62553792-62553811	MS.gene50181:CDS	30.0%
AGATTTAGGTGATGAAATGA+AGG	-	chr5.4:62554029-62554048	MS.gene50181:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	62553040	62559010	62553040	ID=MS.gene50181
chr5.4	mRNA	62553040	62559010	62553040	ID=MS.gene50181.t1;Parent=MS.gene50181
chr5.4	exon	62558918	62559010	62558918	ID=MS.gene50181.t1.exon1;Parent=MS.gene50181.t1
chr5.4	CDS	62558918	62559010	62558918	ID=cds.MS.gene50181.t1;Parent=MS.gene50181.t1
chr5.4	exon	62556086	62558371	62556086	ID=MS.gene50181.t1.exon2;Parent=MS.gene50181.t1
chr5.4	CDS	62556086	62558371	62556086	ID=cds.MS.gene50181.t1;Parent=MS.gene50181.t1
chr5.4	exon	62555828	62555945	62555828	ID=MS.gene50181.t1.exon3;Parent=MS.gene50181.t1
chr5.4	CDS	62555828	62555945	62555828	ID=cds.MS.gene50181.t1;Parent=MS.gene50181.t1
chr5.4	exon	62555611	62555696	62555611	ID=MS.gene50181.t1.exon4;Parent=MS.gene50181.t1
chr5.4	CDS	62555611	62555696	62555611	ID=cds.MS.gene50181.t1;Parent=MS.gene50181.t1
chr5.4	exon	62554911	62555155	62554911	ID=MS.gene50181.t1.exon5;Parent=MS.gene50181.t1
chr5.4	CDS	62554911	62555155	62554911	ID=cds.MS.gene50181.t1;Parent=MS.gene50181.t1
chr5.4	exon	62553972	62554058	62553972	ID=MS.gene50181.t1.exon6;Parent=MS.gene50181.t1
chr5.4	CDS	62553972	62554058	62553972	ID=cds.MS.gene50181.t1;Parent=MS.gene50181.t1
chr5.4	exon	62553767	62553866	62553767	ID=MS.gene50181.t1.exon7;Parent=MS.gene50181.t1
chr5.4	CDS	62553767	62553866	62553767	ID=cds.MS.gene50181.t1;Parent=MS.gene50181.t1
chr5.4	exon	62553395	62553602	62553395	ID=MS.gene50181.t1.exon8;Parent=MS.gene50181.t1
chr5.4	CDS	62553395	62553602	62553395	ID=cds.MS.gene50181.t1;Parent=MS.gene50181.t1
chr5.4	exon	62553040	62553185	62553040	ID=MS.gene50181.t1.exon9;Parent=MS.gene50181.t1
chr5.4	CDS	62553040	62553185	62553040	ID=cds.MS.gene50181.t1;Parent=MS.gene50181.t1

Gene Sequence

>MS.gene50181

ATGGAGGTTGATGGTGATAAGAGTGATAATGGAAATCGAAAAAGGAAAAGAGGGTTATTGAAAAGAAAGCTTCTTGTCAATGAAAAAGTTGAGGTAATATTTGTGATTTCTGTTTTTTTTCTTCATTTTTGATAAAGATTTGATTTTTATTTGGTTATGAATTTCTGGGTTGTTGATTTTGTATTTGGTTTTGTGATTTGATGTTCTTAGGGTTTCTTTGAGGGGTTTTTTACACAATTGTTATGATTCTTTTATATCAATAATTTTCTTTTGTTTCTCTGTTTATGATCCTTTGATTTTCTTTTAATTCTGTTGTTGATTTATGGTTTCGATGGATTTTGATGTTTCTCCTGTATGATTTTTGTTTTCATAATTCACATTTGCTAAAATTGTTTTGATTTTTCTTGTTTTACGCTGTTTTGGATTTCATGCAAGTGATGAATTGGGAATAAGATAGATACTGTTTATGGTAATGTGGACTCAAATGTTATTTATTTCCTTTATCAACACTTTTCCATTTTTTTAACAATTGTTTTTTGATTTGAGATACATGCATGTTGTGTGAATGGTGATTTCCTTCATCTGATCTTTCCACTTGAAAGAATTTTTTATAAAAAGTTTATTTTTTCAACTTTTCAGTTGAGGAGTTTGGAAGATGGTTTTCTGGGATCATGGCATCCAGGGAAAATTATCCGCTGTGGGAGGAAGACGCGTTATGTCAGATATGACAATATCCTTGATGATGAAGAATCAGATTATCTTATTGAAGTGGTGGATGTTTCTAATGTTTTGGATGGTGTTAATTCTTCATCTGCTTTAGATTGTGGCTATCAGCGAGGCCTGATTAGACCGTTACCGCCTCCAATTGAGTTACGGATAAAGGATCTTCCATTTGGACAATGTGTTGATGTGAAATATCAGGATGCTTGGTGGGAAGGTGTGATATTCGATCGCTGCGATGGAATGAAGGATAGGAGCATATTCTTCCCAGATTTAGGTGATGAAATGAAGGTTGGAGTTAAACAACTGAGGATCACTCAGGATTGGGATGAATCAACTGAGAATTGGTTACCAAGAGGGAAATGGGTTTTTCTTGAGCTGTTTGAGGAGTGTGAGAGGGTATCCTATGTTGCAGTCTCGGTGAAACAGATTTGGTATGATATAAGGATGAGAAAGGATTTTGCTGAAACCATTAGAGAATGGACTTGTAATGTGAAGGAGTTGTGGAGAGACCTTGTTGTTGAGGTAATTGGTGATTACTATACCCTCACATTAAGCGAAGTTCGCCCCGCCTTAAACCTTCCAAACAATTTACTCGAGGGAGAGTCATTTGAACCAACTGATAATGTTCATTGTGACGCTGCTAATCAAGGCGATGCATTTGGTTCTGACATTGCAATATCTGATAAGCCTGAGGAAAACGCCGACTCCTCAAACTTGTTGGTTACTGATCAAAACTGTGGCAGCACAAGCATCATTCCTTTGGTGGATGAGGACTTTGAGAAGGAAATTCTTTTACATGAAGAGCTTGAATCAGATAAAAATCAAATGGTGGATGTGGAATTTGAGAAGGAAATTCTTTTACATGAAGAGCTTGAATCAGATAAAAATCGAAAACGGAGACGTTCTAAGTCCATAATTTGGAAGCCTTTGGAACTGTCTGAAGTTGAATTCTGTCCTGAAGTTATTGATGAGTATGCACTTGGATGTAGGAGCAAGACGGTTCGGGAGCTTTTGAAGACGAAAGTAAGAAAACATCTTGTTTATCTTGGATGGACAATTGAATGGACCGAAAACAATACTCCTCCACATAGACGATACCGGTACAAGTCTCCGGATAAACTTAATCCAAAGTTTTACACTTCGATTTTTCAAGTTACTAAAATTTTGCAGGAGGATCCTAACATGAACTCTGGACCACCTCAAATTGACAGCAATCTTTCACATCTGCTCTCTGATCCACCCCAAATGAGCCAAGGTTTCAACGTTTGTCCTCCAACCAATGAACCTTCTCCAGTTAAATTTCAAGTTGAACCAGAATTTTGTCCTCTTGGCATCGTGAAATACTACTGTCACTCATTAGAGAGAAACTCGGCTGATAAGAGAAAGTGGAAATTGAAGGCGAAAAAACATCTGTTAGCAGAAGGATGGATGATTGACTACCCAACTGAAAGAAGGAAGACTACTTTGTACAAGTCTCCACAAGATCAGTGTTTAGGCACACTTCAAGGTGCATGCAGACTATACCTTAAAGAAAAGATTCCCGAATGGACCAATTCTGATCATGGTGGTGATGATGATCTACTTTCGAGTGTGTCCCAATTGCTTCAAAAAGAGCCTGAATTACGTACTATAGATGGTTCACCACCAACTGCAAAGAGAAACCGTAAACGCACAAGGACTTCAAAGGCTAGCACACAAAAGGACTTAGAGAGTGAAGTACTTACGCGGGTGCTAAGATCAAGCAAAAGGGTGCAGAAGGTTTTGGGTTCATCATATCAAAAGCCTCAAAATATTCTGTCTTGGTTAATAGATTGCAACATTGTGTTGCCAAAGTACAAGGTCTTTTACTGGGAAACCGAAGGAGGAAACTCTCCTACGTTTGAAGGGAGGATAACTCGCGAAGGAATTAGGTGCACCTGTTGTCAGAATCTATATGGACTCAGCGGCTTTGCAAACCATGCTGGTGGAAGCAGTAACTGCAGACCTTCGGCTTGTATATTTTTGAAGGATGGTAGGTCACTGTTAGATTGCATGAGGGAAGTAATGCAAGATCACAGGACAAGAGAAATCACGGAGAAACCGCACAATGATCTGTTTGAAGGTGAAAATGACAACATTTGTTCTGTTTGTAACTACGGTGGTGAACTCATTTTATGCGACCAATGCCCCTCGGCGTATCATAAGAATTGTCTTAATTTGGAGGTAGATTATTAACAAGTTTCCTTTTCAATCATAACATATTAAGTTCTATCTTGTTGCTATATTTCCTTTCCTTTTTGTTTCCATGTAACTCCTTCAATTCAGGGCTTCATGCCATATTAAGTTTCATTCTGCATGTGTAGGGCATCCCTGATGGTGATTGGTTTTGTCCATCATGCCGTTGCGGGATTTGCGGCCAAAATAAAATTGAAGAGACCGAGGATGGACACTTTCTTACTTGCATTCAGTGTGAACATAAATGTGAGCTGATATATAGATTTTATTCAATATTCCATCCATTCCTTTGCGATGTGTTTTAGTTTGTGTGAATAACTACATATTTTGACCAATAAAAAATTTGACCAATGAAAAATGTCTTCAACTATATGTAGATCATGTTGAGTGCCTCAGAAATGAAGAAAAAGATGATTCGAGACGATATATGAAAAATTGGCTCTGTGGAGAAGAGTGTGAACGGGTAAGTTAATTCCACACCCCCCCCCCCCCCCCCCCCCCCCCCTTTTTTATTCTAGTTTAGAAATATTTTCATCTCATTTTGCATGAAGAATTGGTTTCTGCTTCTATTTATTCTGATTTGACATTCGTTGGAAGCATTACTTTACCATTTTTATTCCTTTATCAAAACTGACTACTCCACATCCAATTCAAACACTTGATACTTTCTAGTCCAACAATGAGCTCTTTATTCAGATCATGGTTGCATCTATTAATGGCTGTCATTTAATAGCTAAGTAGCAAGTCGAGAATGCATGATTTTTGGTGATTGGTAAGTGTTAAGACATCAAAAGCAAAACAGATTTAAGTTTGATGCTAGGGAAATATTTAAGGCCTTATGACTTAAATATACACTGCTAGAATTATGTTTTATGACAATGGTTTTACTCTACATTTACGCGTATGCACTTTTTGCAGGTCTATACGGGGCTCCAAAATCTATTAGGAAAGCCGGTTTTAGTGGGGGCGGACAATCTAACTTGGACATTGGTGAAGTATGTCAACTCTGAGACTTGCGGTGTTGGCGGTGCTGAAAGTGACTTAGTGGCGGAGAATTACAGCAAACTGAGTGTTGCACTCTCGGTGATGCATGAATGTTTTGAGCCCCTGCATAACCCTTTCTCTAGCAGAGATATCGTTGAAGACGTTATATTCAACCAAAGGTTAGACATTCTTATCCTTCAATTTATGTTTATCATTTTTTTATGATTATTTGCAAACTTTGTTTGTCGCATACTCACAATTCATGCATATAACATCATGAGTTGAACCTGCTGAAATAGCTTTGCTTACCTTCAATTTTGAGCCTTTTCCATTACACCATGGTGGTAATATTCAGGTAACAACTGAAAGTAATCAATTCTTTAATCTACCAACTTTATCATCAAGAAAAACAAACTCTACTATTTTTATACAATGCATAGATATGTTAGAATTTAGGATTTCTTTTGTTGCATATATAGTGTTAGGGCAAAGTTTGCGGCTTTATGTTTATCATGCAGGATGGAATTTCTTGGACCGTAACATATTTATTTGAAGATACTAATAAAATAGTTTCCTTATTTTTATGTACGCATTTCTTCTCGTCCAACTTATTGTGCAAAATCTATGCCTATAGATGGCATCATGATTAGTGAGCCGGTGTTTGAGGAGTAAACAAAGTATTTCGGCTCATGTTGAGTATTTATTACGATTTTGGTTATTTGATCCAAATATGATTCTAATGGATATATCATATAGCTTTTAACTCCTTCCGTGCTTAGATTTTTTTTCAATCAAACAAGATGGCATGCAAAAGTATCACTATAGGTTTTAAGATTCTAACTATGTTTGCACCTCAAAACCTTAGTTATGATTGTATTTGTTTCATGTACATCTTTGAGTCCATTCACTGATGGATTTGTTAACATGTTTACATTGTTTGTGATCATGAAATTTTATCTATAACTTGTCCTGCATTATTGTTATATTCACACTGCGCCAATGGCGGATGTATGTACTTTGGAACCTCGTAGGTCTGAGCTCAATCGACTAAACTTCCAGGGTTTCTATACTGTACTTCTTGAGAGAAATGAAGAATTGATTAGTGTGGCGACTGTGAGGTGAGTATAGGATCTGCTACTACTAAGAATCAAATAAAATAATAGATTATGCATGATTCTATGACTGCATTTCAAGTTTCCATAACTTGGCATTTCATTTTTTAGGATATTTGGAGAGAAGATAGCAGAAGTGCCCCTCGTAGGTACCAGATTTCAGTACAGACGACTTGGAATGTGTCGCGTTTTAATGGATGAGCTCGAAAAGGTGACATAAAAATCAAAGTTTGTCATTTTATCTGGTTGTTTTTGTGAACTATATTAAATTATCTTGATACGGCCTGTGGCATTTGAATTGTAATTCATTTCTATTGTGTAAGTTATTCCAAGTTTATTATCAAATTTCTCACTTTAATTTTTTTCTTTCATCAGAAGCTAAAGCAACTGGGAGTGGAAAGACTAGTCTTGCCTGCTGTTCCCGGTGTGCTAGACACATGGACGAATTCTTTTGGTTTTGAGCAGATGACGAATTTTGAGAGGTCAAAGTTTCTGGACTATTCTTTCCTAGACTTTCAAGGAACTGTCATGTGTCAGAAACTGTTGACAAGATTTCCATCTCCGGAATCGGTTGTAACAAGAGGTTTGTAGCTTCTTATGATTCCTATATTAGCTGTTAGTTTGGCATAATCACAATGTGGTAACGTTATTGTCGCAATAACTACACTTTAGAGCTTTAACAAATTCAAAGTGTCATTGTGATATCGCGAAACTCACCGACAATCCAAATATGCACATAATTAAAGAGTTTCCTCACTGATTGGATTATAACCTTATTTTTTCATATTGCAGACGCGACTCCAAAACAACAAGATGGTAACTTCTCTGTTAAATGTAAAATTAATTTTGAAAAGGCTAGTCCAGTATCTGAAGTGGATCAAGCAGAAGGGATTAGCAAAAGAGGAATGATGGATCTACAAGTTTGGAGACCTTCCTAA

Protein sequence

>MS.gene50181.t1

MEVDGDKSDNGNRKRKRGLLKRKLLVNEKVELRSLEDGFLGSWHPGKIIRCGRKTRYVRYDNILDDEESDYLIEVVDVSNVLDGVNSSSALDCGYQRGLIRPLPPPIELRIKDLPFGQCVDVKYQDAWWEGVIFDRCDGMKDRSIFFPDLGDEMKVGVKQLRITQDWDESTENWLPRGKWVFLELFEECERVSYVAVSVKQIWYDIRMRKDFAETIREWTCNVKELWRDLVVEVIGDYYTLTLSEVRPALNLPNNLLEGESFEPTDNVHCDAANQGDAFGSDIAISDKPEENADSSNLLVTDQNCGSTSIIPLVDEDFEKEILLHEELESDKNQMVDVEFEKEILLHEELESDKNRKRRRSKSIIWKPLELSEVEFCPEVIDEYALGCRSKTVRELLKTKVRKHLVYLGWTIEWTENNTPPHRRYRYKSPDKLNPKFYTSIFQVTKILQEDPNMNSGPPQIDSNLSHLLSDPPQMSQGFNVCPPTNEPSPVKFQVEPEFCPLGIVKYYCHSLERNSADKRKWKLKAKKHLLAEGWMIDYPTERRKTTLYKSPQDQCLGTLQGACRLYLKEKIPEWTNSDHGGDDDLLSSVSQLLQKEPELRTIDGSPPTAKRNRKRTRTSKASTQKDLESEVLTRVLRSSKRVQKVLGSSYQKPQNILSWLIDCNIVLPKYKVFYWETEGGNSPTFEGRITREGIRCTCCQNLYGLSGFANHAGGSSNCRPSACIFLKDGRSLLDCMREVMQDHRTREITEKPHNDLFEGENDNICSVCNYGGELILCDQCPSAYHKNCLNLEGIPDGDWFCPSCRCGICGQNKIEETEDGHFLTCIQCEHKYHVECLRNEEKDDSRRYMKNWLCGEECERVYTGLQNLLGKPVLVGADNLTWTLVKYVNSETCGVGGAESDLVAENYSKLSVALSVMHECFEPLHNPFSSRDIVEDVIFNQRSELNRLNFQGFYTVLLERNEELISVATVRIFGEKIAEVPLVGTRFQYRRLGMCRVLMDELEKKLKQLGVERLVLPAVPGVLDTWTNSFGFEQMTNFERSKFLDYSFLDFQGTVMCQKLLTRFPSPESVVTRDATPKQQDGNFSVKCKINFEKASPVSEVDQAEGISKRGMMDLQVWRPS