AlfalfaGEDB | MS.gene50325

M. sativa cultivar XinJiangDaYe / MS.gene50325

KEGG: -
GO: GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
PFAM: PF03101.18 PF04434.20

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50325.t1	RZB50232.1	68.8	560	106	6	5	507	7	554	1.50E-208	735.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50325.t1	Q9SY66	43.7	293	153	3	78	361	30	319	8.3e-62	239.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50325.t1	A0A445FMV5	68.8	560	106	6	5	507	7	554	1.1e-208	735.7

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene50325.t1	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50325.t1	MTR_4g035820	59.125	526	146	11	1	476	1	507	0.0	575
MS.gene50325.t1	MTR_2g035140	51.237	283	110	6	220	500	12	268	5.39e-86	268
MS.gene50325.t1	MTR_3g450850	38.462	247	135	5	89	324	46	286	1.64e-46	166
MS.gene50325.t1	MTR_6g014355	37.154	253	138	4	154	397	5	245	8.67e-43	153
MS.gene50325.t1	MTR_6g051425	30.337	267	159	8	91	344	18	270	7.32e-31	123
MS.gene50325.t1	MTR_8g447350	60.204	98	34	2	354	451	39	131	4.45e-30	116
MS.gene50325.t1	MTR_1g070355	32.773	238	147	6	96	329	80	308	1.72e-28	119
MS.gene50325.t1	MTR_5g017720	33.898	177	78	4	350	520	21	164	1.88e-20	89.7
MS.gene50325.t1	MTR_3g012160	41.935	93	54	0	233	325	1	93	1.08e-18	81.6
MS.gene50325.t1	MTR_5g076070	24.815	270	180	8	59	324	32	282	5.44e-17	82.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50325.t1	AT1G10240	44.604	278	148	2	87	361	45	319	1.98e-74	249
MS.gene50325.t1	AT1G10240	44.604	278	148	2	87	361	45	319	1.98e-74	249
MS.gene50325.t1	AT5G28530	38.676	287	162	3	89	364	54	337	5.01e-56	200
MS.gene50325.t1	AT5G28530	38.676	287	162	3	89	364	54	337	5.01e-56	200
MS.gene50325.t1	AT5G28530	38.676	287	162	3	89	364	54	337	5.57e-56	199
MS.gene50325.t1	AT5G28530	43.716	183	99	2	89	270	54	233	2.70e-39	152
MS.gene50325.t1	AT5G28530	43.716	183	99	2	89	270	54	233	3.06e-39	152
MS.gene50325.t1	AT4G38180	30.970	268	174	4	87	350	70	330	3.16e-29	123
MS.gene50325.t1	AT2G27110	30.418	263	147	7	90	350	49	277	1.60e-24	108
MS.gene50325.t1	AT2G27110	30.418	263	147	7	90	350	49	277	1.60e-24	108

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Find 71 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGGAGACGTCTTAAAGG+TGG	0.635208	4.4:+78187371	MS.gene50325:CDS
AAAGAAATAGAGAATCAACA+AGG	0.743349	4.4:+78186451	MS.gene50325:CDS
AAAGACAACATTGATTGGTT+GGG	0.472431	4.4:-78187261	None:intergenic
AAATGGTAATGTGTTTGTGT+TGG	0.538048	4.4:+78187028	MS.gene50325:CDS
AACAAAATGGAATTGTGAGT+AGG	0.593662	4.4:+78186364	MS.gene50325:CDS
AACACATGTGGATTATGCAG+GGG	0.747277	4.4:+78187416	MS.gene50325:CDS
AACCAAAAGGCCGTCCCAAA+CGG	0.473805	4.4:+78187354	MS.gene50325:CDS
AAGAGGTATGCACGTCAACA+TGG	0.594612	4.4:+78186312	MS.gene50325:CDS
AATCATCTTCTGAGATAGTA+CGG	0.550778	4.4:-78186617	None:intergenic
AATGGTAATGTGTTTGTGTT+GGG	0.431088	4.4:+78187029	MS.gene50325:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAAAATGGAATTGTGAGT+AGG	+	chr4.4:78186364-78186383	MS.gene50325:CDS	30.0%
AATTCTTACGAAATGCCATT+TGG	+	chr4.4:78186978-78186997	MS.gene50325:CDS	30.0%
AATTGTTAGAAGGGATTTCA+TGG	-	chr4.4:78187190-78187209	None:intergenic	30.0%
AGGAAAATTTGACCAACAAA+AGG	-	chr4.4:78186264-78186283	None:intergenic	30.0%
ATACACTTGATGAAGAAAGA+CGG	+	chr4.4:78186865-78186884	MS.gene50325:CDS	30.0%
ATACCCACAAAAATTCCAAA+TGG	-	chr4.4:78186996-78187015	None:intergenic	30.0%
ATGATATATTTCCAACTGAG+TGG	+	chr4.4:78186520-78186539	MS.gene50325:CDS	30.0%
GGTAGATTCATCAAACAAAA+TGG	+	chr4.4:78186351-78186370	MS.gene50325:CDS	30.0%
GGTATGAATAATCATGGAAA+TGG	+	chr4.4:78187011-78187030	MS.gene50325:CDS	30.0%
TACTAATAGCAGAAAACACA+CGG	+	chr4.4:78187064-78187083	MS.gene50325:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	78185994	78187773	78185994	ID=MS.gene50325
chr4.4	mRNA	78185994	78187773	78185994	ID=MS.gene50325.t1;Parent=MS.gene50325
chr4.4	exon	78185994	78187517	78185994	ID=MS.gene50325.t1.exon1;Parent=MS.gene50325.t1
chr4.4	CDS	78185994	78187517	78185994	ID=cds.MS.gene50325.t1;Parent=MS.gene50325.t1
chr4.4	exon	78187660	78187773	78187660	ID=MS.gene50325.t1.exon2;Parent=MS.gene50325.t1
chr4.4	CDS	78187660	78187773	78187660	ID=cds.MS.gene50325.t1;Parent=MS.gene50325.t1

Gene Sequence

>MS.gene50325

ATGAATGAAGTTAGTGCTAGAGATATGAGCAACAATAATACAAATTTTGATTTGAATGTTCAATATCCAGTAGAAGAAGTTACTTTAGAAAATGGTAATTCTGAGTCAATCGATGAACATTTTGATCTTAACATTGTGGCAAGAGATGTGTTAGAAATTGATGAAATCGGAATTTCTGAGAAAGAAAATTGTGTGCCTTCTAGTCAAGATGTTGAGATGAATGGGTTTTTAGAAGAAAGTGACGAAGACATGGAGAAAAATATTGTTCCTTTTGTTGGTCAAATTTTCCTAAGTGAGGAAGAAGCATTCGTTTTTTATAAGAGGTATGCACGTCAACATGGATTCTCGGTTCGAAAAGGTAGATTCATCAAACAAAATGGAATTGTGAGTAGGCGTGATTTCTTTTGTCACCGTGAAGGTAGAACCTCTTTGAAAATCATGGAACCATCAAAAGAACAAAGAAATAGAGAATCAACAAGGTGCGAATGTAAAGCTCATTTACGAATTTTATTGCAGAAGTCTGATAATGATATATTTCCAACTGAGTGGCGAGTTACAAAGTTTGTTGTTGAACATAACCATACTTTGTTGTCTCAATCAGAAGTGCGCTTTTTACCGGCATACCGTACTATCTCAGAAGATGATTATGAACGCATTTTTTTGTTAAAAGAAGGCGGACTTTTGGTTAGACAAATAATGCGTGTCATTGAACTAGAGAAAAATGTGAAGCATGGTTATCTTCCTTTTATTGAAAGGGACGTTCGCAATCTTTTTTCAAAAGCGAAGAAACAAGTTGAAAGAAGTGATGTTATTGATCTTCTTAAGTACTTTGAGGATGCAAAAAAGAGGTGTTCTAAATTTCAGTATGCATATACACTTGATGAAGAAAGACGGTTAGAACATATTTTTTGGTCCCCTGCTTCTTGTTATGATTGGTACCAAGAATATGGTGATGTTGTTGTGTTTGACACTACATACAAAGTAAATTCTTACGAAATGCCATTTGGAATTTTTGTGGGTATGAATAATCATGGAAATGGTAATGTGTTTGTGTTGGGGTATTATAAAGATACTAATAGCAGAAAACACACGGTCTTTTGGGATGGTAAAATAGCTACATGTACTTGTAAGCACTTTGAGTTCTTAGGGATTTTATGCCGTCACATTCTAAGTATTTTTCTTCGTAAAGATTGCCATGAAATCCCTTCTAACAATTTGCCATCACGATGGCAAATTCAAGCATCACATGACGATGATGTACTAGATGCCCAACCAATCAATGTTGTCTTTGAGGAACAAGCAATAGATTGCAACATTGAAGCTCATTCACAATGTGATGTTCGTTGTCCTCCAATGTCCAAACCAAAAGGCCGTCCCAAACGGAGACGTCTTAAAGGTGGAAAAGAGCTTTCGCATAACATGAACACATGTGGATTATGCAGGGGTGTAGGACATAATATTGGTACATGTCCTTTAAAAGAAAATATTCAGTGTTCTGCTCACAACAAAAGCAAGAAGAAAAAAGTTTGTGAAGATGAAAATTTAAATCCAATTCTTCTTTCAAAAGTTTAGGTACATTTTTATTTTTCAATTTATTAATTACATTATTTAGATTTCTAGTTCGTTAGTTAAAATTATATAGCTTGTTTCTGTTTTTTTATCGCAGGAATTTGCTGGGAATACAACATGGAAGAACACTTTTAGGAATTTGCTGGGAATGCACGGGAATAACACTTTTATGACATATAAGCATGAAGGCCATCTATCTCTTTTGGACTAA

Protein sequence

>MS.gene50325.t1

MNEVSARDMSNNNTNFDLNVQYPVEEVTLENGNSESIDEHFDLNIVARDVLEIDEIGISEKENCVPSSQDVEMNGFLEESDEDMEKNIVPFVGQIFLSEEEAFVFYKRYARQHGFSVRKGRFIKQNGIVSRRDFFCHREGRTSLKIMEPSKEQRNRESTRCECKAHLRILLQKSDNDIFPTEWRVTKFVVEHNHTLLSQSEVRFLPAYRTISEDDYERIFLLKEGGLLVRQIMRVIELEKNVKHGYLPFIERDVRNLFSKAKKQVERSDVIDLLKYFEDAKKRCSKFQYAYTLDEERRLEHIFWSPASCYDWYQEYGDVVVFDTTYKVNSYEMPFGIFVGMNNHGNGNVFVLGYYKDTNSRKHTVFWDGKIATCTCKHFEFLGILCRHILSIFLRKDCHEIPSNNLPSRWQIQASHDDDVLDAQPINVVFEEQAIDCNIEAHSQCDVRCPPMSKPKGRPKRRRLKGGKELSHNMNTCGLCRGVGHNIGTCPLKENIQCSAHNKSKKKKEFAGNTTWKNTFRNLLGMHGNNTFMTYKHEGHLSLLD