AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50397


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50397.t1 MTR_6g477950 77.273 88 19 1 171 257 64 151 1.66e-41 144
MS.gene50397.t1 MTR_5g071410 33.977 259 156 4 1 253 22 271 8.73e-33 120
MS.gene50397.t1 MTR_7g117585 44.762 105 57 1 150 253 44 148 5.53e-22 89.4
MS.gene50397.t1 MTR_8g069780 48.750 80 41 0 172 251 76 155 2.44e-18 79.7
MS.gene50397.t1 MTR_3g117130 48.750 80 41 0 172 251 199 278 1.06e-16 78.2
MS.gene50397.t1 MTR_0131s0030 34.615 130 77 3 113 242 28 149 5.86e-15 70.9
MS.gene50397.t1 MTR_2g437520 46.988 83 41 1 172 251 121 203 1.35e-14 70.9
MS.gene50397.t1 MTR_2g012700 28.448 116 79 2 139 252 7 120 1.95e-13 65.9
MS.gene50397.t1 MTR_2g062330 33.333 102 65 2 150 251 40 138 3.31e-13 65.9
MS.gene50397.t1 MTR_6g018740 48.276 58 30 0 172 229 52 109 3.75e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 58 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTGATAAGTTTGAATTAA+AGG 0.118029 4.4:+79134997 MS.gene50397:CDS
ACCAACGTCATCCACTTTCT+TGG 0.252631 4.4:-79135681 None:intergenic
TGTTTGTTCATGTGGTTCAT+AGG 0.275003 4.4:+79135865 MS.gene50397:CDS
CAAACTCATCTCTACCTAAC+TGG 0.330629 4.4:-79134946 None:intergenic
TAAGATTGACATACCTCGTC+TGG 0.345367 4.4:-79134626 None:intergenic
GTTTGTTCATGTGGTTCATA+GGG 0.378998 4.4:+79135866 MS.gene50397:CDS
CACTTTGTGTTCAAAGTGAT+AGG 0.388587 4.4:-79135275 None:intergenic
TCCGTTCAGCGGTGTTGAGC+TGG 0.392349 4.4:-79135734 None:intergenic
AGTAGAAAACAATGTGGAAT+TGG 0.392437 4.4:+79135099 MS.gene50397:CDS
AGAGGTTGTTGACAACAAAA+AGG 0.410832 4.4:+79135156 MS.gene50397:CDS
AGAGGGATAGTTATCCAGTT+AGG 0.432165 4.4:+79134932 MS.gene50397:CDS
AGGATGGTCAAAATTTGTTA+AGG 0.437420 4.4:+79135782 MS.gene50397:CDS
AGATGTTATTGATATAAGTT+CGG 0.453836 4.4:+79135186 MS.gene50397:CDS
GCTATGTCTAAGCGTGTTCT+CGG 0.457464 4.4:+79135304 MS.gene50397:CDS
CAGTTAGGTAGAGATGAGTT+TGG 0.459071 4.4:+79134947 MS.gene50397:CDS
GATTTCTTCATAAGAAATCA+AGG 0.490357 4.4:+79134863 MS.gene50397:CDS
GGTTGACATGGATACTGATA+AGG 0.495581 4.4:+79135701 MS.gene50397:CDS
AAATCAAGATGTCGAAGAAG+AGG 0.514225 4.4:+79135246 MS.gene50397:CDS
ACCTCTCTAGACACACAGTT+GGG 0.515669 4.4:-79135648 None:intergenic
TCTTCATAAGAAATCAAGGC+AGG 0.519683 4.4:+79134867 MS.gene50397:CDS
TTCATCTTTGTTCCGTTCAG+CGG 0.520880 4.4:-79135745 None:intergenic
TTCAAACTTATCAGCAATTG+CGG 0.521252 4.4:-79134990 None:intergenic
TTAACAAGCCTGGATATGCT+AGG 0.521703 4.4:+79134897 MS.gene50397:CDS
ATAGGGGTAACATTGCTTGA+GGG 0.527322 4.4:+79135883 MS.gene50397:CDS
CTCGCGAAATTTGAATATCT+CGG 0.529020 4.4:+79135028 MS.gene50397:CDS
CTCTTGTACGACATGTGCAT+TGG 0.540397 4.4:-79135137 None:intergenic
TCACCATCCCTAGCATATCC+AGG 0.541689 4.4:-79134905 None:intergenic
TGATATAAGTTCGGATGAAG+AGG 0.542008 4.4:+79135195 MS.gene50397:CDS
CAATGTGGAATTGGTCCCAC+AGG 0.543202 4.4:+79135108 MS.gene50397:CDS
AGGCTTCAAATTAACAAGCC+TGG 0.546240 4.4:+79134887 MS.gene50397:CDS
GTGGATGACGTTGGTTGACA+TGG 0.551372 4.4:+79135689 MS.gene50397:CDS
TAAGGATCGCCGCTTAAAAG+AGG 0.551825 4.4:+79135800 MS.gene50397:CDS
TCTATTCTGATCAGCCAAGA+AGG 0.552897 4.4:-79135074 None:intergenic
TAGGAGAGGATACAAGTGGA+TGG 0.555529 4.4:+79134839 MS.gene50397:intron
ACATGAACAAACAAAACTCT+AGG 0.556379 4.4:-79135855 None:intergenic
ACTTTGTGTTCAAAGTGATA+GGG 0.559481 4.4:-79135274 None:intergenic
GCGTGTTCTCGGACAACCAA+TGG 0.563243 4.4:+79135315 MS.gene50397:CDS
CATAGGGGTAACATTGCTTG+AGG 0.567582 4.4:+79135882 MS.gene50397:CDS
TCCCAACTGTGTGTCTAGAG+AGG 0.572226 4.4:+79135647 MS.gene50397:CDS
CTAGGGATGGTGATTGCAGA+GGG 0.580162 4.4:+79134915 MS.gene50397:CDS
TAACAAGCCTGGATATGCTA+GGG 0.582056 4.4:+79134898 MS.gene50397:CDS
AAGATTGACATACCTCGTCT+GGG 0.584454 4.4:-79134625 None:intergenic
AACCTCTCTAGACACACAGT+TGG 0.586186 4.4:-79135649 None:intergenic
TATTCAAATTTCGCGAGAGT+AGG 0.600261 4.4:-79135022 None:intergenic
CAATGCACATGTCGTACAAG+AGG 0.602899 4.4:+79135138 MS.gene50397:CDS
GCCAGCTCAACACCGCTGAA+CGG 0.609154 4.4:+79135733 MS.gene50397:CDS
TTTGTTCATGTGGTTCATAG+GGG 0.613718 4.4:+79135867 MS.gene50397:CDS
TGTGCATTGGCAGCAACCTG+TGG 0.615952 4.4:-79135124 None:intergenic
AAGGATCGCCGCTTAAAAGA+GGG 0.619565 4.4:+79135801 MS.gene50397:CDS
TATAAGTTCGGATGAAGAGG+TGG 0.631039 4.4:+79135198 MS.gene50397:CDS
AAGCCTGGATATGCTAGGGA+TGG 0.631545 4.4:+79134902 MS.gene50397:CDS
TGAACAAACAAAACTCTAGG+AGG 0.641689 4.4:-79135852 None:intergenic
GCTAGGGATGGTGATTGCAG+AGG 0.652278 4.4:+79134914 MS.gene50397:CDS
TCCAAGAAAGTGGATGACGT+TGG 0.665396 4.4:+79135680 MS.gene50397:CDS
AACCATGGTGAACCCAGACG+AGG 0.693320 4.4:+79134613 None:intergenic
GTGCATTGGCAGCAACCTGT+GGG 0.698395 4.4:-79135123 None:intergenic
TAGACAAGTAGAAAACAATG+TGG 0.704557 4.4:+79135093 MS.gene50397:CDS
TACCTCGTCTGGGTTCACCA+TGG 0.713572 4.4:-79134615 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAAATGTTAATGATAAA+GGG - chr4.4:79135364-79135383 None:intergenic 10.0%
!! CATATGAAATTAATTAAAAT+AGG + chr4.4:79135423-79135442 MS.gene50397:intron 10.0%
!!! GTATATTTTTCAAAATAAAT+CGG - chr4.4:79135463-79135482 None:intergenic 10.0%
!! CATATAAATGTTAATGATAA+AGG - chr4.4:79135365-79135384 None:intergenic 15.0%
!! CTTTATCATTAACATTTATA+TGG + chr4.4:79135363-79135382 MS.gene50397:intron 15.0%
!! TTATTTAAATAACTAACCAT+TGG - chr4.4:79135334-79135353 None:intergenic 15.0%
!!! TTATTTTGAAAAATATACTC+TGG + chr4.4:79135465-79135484 MS.gene50397:intron 15.0%
!! ACACTAAAATTATAATACTC+AGG - chr4.4:79134806-79134825 None:intergenic 20.0%
!! AGATGTTATTGATATAAGTT+CGG + chr4.4:79135186-79135205 MS.gene50397:CDS 20.0%
!! CATTACTCTTTATATTTGTA+TGG + chr4.4:79135397-79135416 MS.gene50397:intron 20.0%
!!! TAGTGTTTTTGTTCATTTTT+AGG + chr4.4:79134820-79134839 MS.gene50397:intron 20.0%
!!! TTTTGTATGGTTATTTGTTT+GGG + chr4.4:79134737-79134756 MS.gene50397:intron 20.0%
! AAAGCAACAAACAACTAAAA+AGG - chr4.4:79135543-79135562 None:intergenic 25.0%
! AACAAAGATGAAAAAGCTTT+GGG + chr4.4:79135756-79135775 MS.gene50397:CDS 25.0%
! GATTTCTTCATAAGAAATCA+AGG + chr4.4:79134863-79134882 MS.gene50397:CDS 25.0%
! GTAACTAAAAACATGTTCAA+TGG - chr4.4:79135055-79135074 None:intergenic 25.0%
! TGCTGATAAGTTTGAATTAA+AGG + chr4.4:79134997-79135016 MS.gene50397:CDS 25.0%
! TTTCAAAATAAATCGGATGT+GGG - chr4.4:79135456-79135475 None:intergenic 25.0%
! TTTGAAAAATATACTCTGGA+AGG + chr4.4:79135469-79135488 MS.gene50397:intron 25.0%
!! GTAACTATATTTTCGTCTAT+AGG - chr4.4:79135518-79135537 None:intergenic 25.0%
!! GTTTTGTATGGTTATTTGTT+TGG + chr4.4:79134736-79134755 MS.gene50397:intron 25.0%
!! TTGATTTTGCTCTTCATTAT+CGG - chr4.4:79135233-79135252 None:intergenic 25.0%
!! TTTTCAAAATAAATCGGATG+TGG - chr4.4:79135457-79135476 None:intergenic 25.0%
!!! TGACAAGCTAATTTTTGATT+TGG + chr4.4:79135824-79135843 MS.gene50397:CDS 25.0%
!!! TTTGGTTTTTTCATGAAGAA+CGG + chr4.4:79134965-79134984 MS.gene50397:CDS 25.0%
!!! TTTTAAAACTTCCAAGAAAG+TGG + chr4.4:79135670-79135689 MS.gene50397:CDS 25.0%
!!! TTTTTGTTCATTTTTAGGAG+AGG + chr4.4:79134825-79134844 MS.gene50397:intron 25.0%
ACATGAACAAACAAAACTCT+AGG - chr4.4:79135858-79135877 None:intergenic 30.0%
ACTTTGTGTTCAAAGTGATA+GGG - chr4.4:79135277-79135296 None:intergenic 30.0%
AGTAGAAAACAATGTGGAAT+TGG + chr4.4:79135099-79135118 MS.gene50397:CDS 30.0%
GAACAAAGATGAAAAAGCTT+TGG + chr4.4:79135755-79135774 MS.gene50397:CDS 30.0%
TAGACAAGTAGAAAACAATG+TGG + chr4.4:79135093-79135112 MS.gene50397:CDS 30.0%
TTCAAAATAAATCGGATGTG+GGG - chr4.4:79135455-79135474 None:intergenic 30.0%
TTCAAACTTATCAGCAATTG+CGG - chr4.4:79134993-79135012 None:intergenic 30.0%
TTTCTTATACATCGTGTCTA+TGG + chr4.4:79135560-79135579 MS.gene50397:intron 30.0%
! AACCATACAAAACTTGTTTG+CGG - chr4.4:79134729-79134748 None:intergenic 30.0%
! CGTCTATAGGTACTTAATTT+TGG - chr4.4:79135505-79135524 None:intergenic 30.0%
!! AGGATGGTCAAAATTTGTTA+AGG + chr4.4:79135782-79135801 MS.gene50397:CDS 30.0%
!!! CATGTTTTTAGTTACCTTCT+TGG + chr4.4:79135060-79135079 MS.gene50397:CDS 30.0%
!!! TAGAGTTTTGTTTGTTCATG+TGG + chr4.4:79135857-79135876 MS.gene50397:CDS 30.0%
AAACAAACCACAGTAACCTT+CGG - chr4.4:79134668-79134687 None:intergenic 35.0%
AAATCAAGATGTCGAAGAAG+AGG + chr4.4:79135246-79135265 MS.gene50397:CDS 35.0%
AGAGGTTGTTGACAACAAAA+AGG + chr4.4:79135156-79135175 MS.gene50397:CDS 35.0%
ATTGAATGAAACTCTTCCGA+AGG + chr4.4:79134649-79134668 MS.gene50397:intron 35.0%
CACTTTGTGTTCAAAGTGAT+AGG - chr4.4:79135278-79135297 None:intergenic 35.0%
CTCGCGAAATTTGAATATCT+CGG + chr4.4:79135028-79135047 MS.gene50397:CDS 35.0%
GGTATATGTGTTTCGTGTTT+AGG + chr4.4:79134758-79134777 MS.gene50397:intron 35.0%
GTTTGTTCATGTGGTTCATA+GGG + chr4.4:79135866-79135885 MS.gene50397:CDS 35.0%
TATTCAAATTTCGCGAGAGT+AGG - chr4.4:79135025-79135044 None:intergenic 35.0%
TCTTCATAAGAAATCAAGGC+AGG + chr4.4:79134867-79134886 MS.gene50397:CDS 35.0%
TGAACAAACAAAACTCTAGG+AGG - chr4.4:79135855-79135874 None:intergenic 35.0%
TGATATAAGTTCGGATGAAG+AGG + chr4.4:79135195-79135214 MS.gene50397:CDS 35.0%
TGTTTGTTCATGTGGTTCAT+AGG + chr4.4:79135865-79135884 MS.gene50397:CDS 35.0%
TTTGTTCATGTGGTTCATAG+GGG + chr4.4:79135867-79135886 MS.gene50397:CDS 35.0%
!! TACCGCAAACAAGTTTTGTA+TGG + chr4.4:79134724-79134743 MS.gene50397:intron 35.0%
!! TTTTTAGGAGAGGATACAAG+TGG + chr4.4:79134835-79134854 MS.gene50397:intron 35.0%
AAGATTGACATACCTCGTCT+GGG - chr4.4:79134628-79134647 None:intergenic 40.0%
AGAGGGATAGTTATCCAGTT+AGG + chr4.4:79134932-79134951 MS.gene50397:CDS 40.0%
AGGCTTCAAATTAACAAGCC+TGG + chr4.4:79134887-79134906 MS.gene50397:CDS 40.0%
CAAACTCATCTCTACCTAAC+TGG - chr4.4:79134949-79134968 None:intergenic 40.0%
CAGTTAGGTAGAGATGAGTT+TGG + chr4.4:79134947-79134966 MS.gene50397:CDS 40.0%
GGTTGACATGGATACTGATA+AGG + chr4.4:79135701-79135720 MS.gene50397:CDS 40.0%
GGTTGTTGACAACAAAAAGG+TGG + chr4.4:79135159-79135178 MS.gene50397:CDS 40.0%
TAACAAGCCTGGATATGCTA+GGG + chr4.4:79134898-79134917 MS.gene50397:CDS 40.0%
TAAGATTGACATACCTCGTC+TGG - chr4.4:79134629-79134648 None:intergenic 40.0%
TATAAGTTCGGATGAAGAGG+TGG + chr4.4:79135198-79135217 MS.gene50397:CDS 40.0%
TCTATTCTGATCAGCCAAGA+AGG - chr4.4:79135077-79135096 None:intergenic 40.0%
TTAACAAGCCTGGATATGCT+AGG + chr4.4:79134897-79134916 MS.gene50397:CDS 40.0%
TTCATCTTTGTTCCGTTCAG+CGG - chr4.4:79135748-79135767 None:intergenic 40.0%
!! AAAAAGCTTTGGGAAGAGGA+TGG + chr4.4:79135766-79135785 MS.gene50397:CDS 40.0%
!! GATGAAAAAGCTTTGGGAAG+AGG + chr4.4:79135762-79135781 MS.gene50397:CDS 40.0%
AACCTCTCTAGACACACAGT+TGG - chr4.4:79135652-79135671 None:intergenic 45.0%
AACTCTTCCGAAGGTTACTG+TGG + chr4.4:79134658-79134677 MS.gene50397:intron 45.0%
AAGGATCGCCGCTTAAAAGA+GGG + chr4.4:79135801-79135820 MS.gene50397:CDS 45.0%
ACCAACGTCATCCACTTTCT+TGG - chr4.4:79135684-79135703 None:intergenic 45.0%
ACCTCTCTAGACACACAGTT+GGG - chr4.4:79135651-79135670 None:intergenic 45.0%
CAATGCACATGTCGTACAAG+AGG + chr4.4:79135138-79135157 MS.gene50397:CDS 45.0%
CTCTTGTACGACATGTGCAT+TGG - chr4.4:79135140-79135159 None:intergenic 45.0%
GCTATGTCTAAGCGTGTTCT+CGG + chr4.4:79135304-79135323 MS.gene50397:CDS 45.0%
TAAGGATCGCCGCTTAAAAG+AGG + chr4.4:79135800-79135819 MS.gene50397:CDS 45.0%
TAGGAGAGGATACAAGTGGA+TGG + chr4.4:79134839-79134858 MS.gene50397:intron 45.0%
TCCAAGAAAGTGGATGACGT+TGG + chr4.4:79135680-79135699 MS.gene50397:CDS 45.0%
! GCTTGTCACCCTCTTTTAAG+CGG - chr4.4:79135812-79135831 None:intergenic 45.0%
AAGCCTGGATATGCTAGGGA+TGG + chr4.4:79134902-79134921 MS.gene50397:CDS 50.0%
CAATGTGGAATTGGTCCCAC+AGG + chr4.4:79135108-79135127 MS.gene50397:CDS 50.0%
CTAGGGATGGTGATTGCAGA+GGG + chr4.4:79134915-79134934 MS.gene50397:CDS 50.0%
TCACCATCCCTAGCATATCC+AGG - chr4.4:79134908-79134927 None:intergenic 50.0%
TCCCAACTGTGTGTCTAGAG+AGG + chr4.4:79135647-79135666 MS.gene50397:CDS 50.0%
! GTGGATGACGTTGGTTGACA+TGG + chr4.4:79135689-79135708 MS.gene50397:CDS 50.0%
GCGTGTTCTCGGACAACCAA+TGG + chr4.4:79135315-79135334 MS.gene50397:CDS 55.0%
GCTAGGGATGGTGATTGCAG+AGG + chr4.4:79134914-79134933 MS.gene50397:CDS 55.0%
! GTGCATTGGCAGCAACCTGT+GGG - chr4.4:79135126-79135145 None:intergenic 55.0%
! TGTGCATTGGCAGCAACCTG+TGG - chr4.4:79135127-79135146 None:intergenic 55.0%
GCCAGCTCAACACCGCTGAA+CGG + chr4.4:79135733-79135752 MS.gene50397:CDS 60.0%
! TCCGTTCAGCGGTGTTGAGC+TGG - chr4.4:79135737-79135756 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 79134617 79135902 79134617 ID=MS.gene50397
chr4.4 mRNA 79134617 79135902 79134617 ID=MS.gene50397.t1;Parent=MS.gene50397
chr4.4 exon 79134617 79134634 79134617 ID=MS.gene50397.t1.exon1;Parent=MS.gene50397.t1
chr4.4 CDS 79134617 79134634 79134617 ID=cds.MS.gene50397.t1;Parent=MS.gene50397.t1
chr4.4 exon 79134842 79135336 79134842 ID=MS.gene50397.t1.exon2;Parent=MS.gene50397.t1
chr4.4 CDS 79134842 79135336 79134842 ID=cds.MS.gene50397.t1;Parent=MS.gene50397.t1
chr4.4 exon 79135642 79135902 79135642 ID=MS.gene50397.t1.exon3;Parent=MS.gene50397.t1
chr4.4 CDS 79135642 79135902 79135642 ID=cds.MS.gene50397.t1;Parent=MS.gene50397.t1
Gene Sequence

>MS.gene50397

ATGGTGAACCCAGACGAGGTATGTCAATCTTAATTGAATGAAACTCTTCCGAAGGTTACTGTGGTTTGTTTTGTTTCACATCATATTTGTTTTATTCGTTGTAAATATACCGCAAACAAGTTTTGTATGGTTATTTGTTTGGGTATATGTGTTTCGTGTTTAGGATTAAAAAAAGCATAAGTAAATCCTGAGTATTATAATTTTAGTGTTTTTGTTCATTTTTAGGAGAGGATACAAGTGGATGGTGATTTCTTCATAAGAAATCAAGGCAGGCTTCAAATTAACAAGCCTGGATATGCTAGGGATGGTGATTGCAGAGGGATAGTTATCCAGTTAGGTAGAGATGAGTTTGGTTTTTTCATGAAGAACGGTGCCGCAATTGCTGATAAGTTTGAATTAAAGGCACCTACTCTCGCGAAATTTGAATATCTCGGACCATTGAACATGTTTTTAGTTACCTTCTTGGCTGATCAGAATAGACAAGTAGAAAACAATGTGGAATTGGTCCCACAGGTTGCTGCCAATGCACATGTCGTACAAGAGGTTGTTGACAACAAAAAGGTGGTTCAAGATGTTATTGATATAAGTTCGGATGAAGAGGTGGATGTGACATCCGATAATGAAGAGCAAAATCAAGATGTCGAAGAAGAGGAAGATCCCTATCACTTTGAACACAAAGTGTCTGCTGCTATGTCTAAGCGTGTTCTCGGACAACCAATGGTTAGTTATTTAAATAAATTTCTTCCCTTTATCATTAACATTTATATGGAGTATCAAAATCATTACTCTTTATATTTGTATGGTGTCATATGAAATTAATTAAAATAGGTAATCACCCCACATCCGATTTATTTTGAAAAATATACTCTGGAAGGTCTTGAATTGCCAAAATTAAGTACCTATAGACGAAAATATAGTTACTACCTTTTTAGTTGTTTGTTGCTTTCTTATACATCGTGTCTATGGTGTCGATATCACTAAAAAGTTACTACTTAATCTAAGTTATTTTAATTTCTTTTTGCTAGCATTTTCCCAACTGTGTGTCTAGAGAGGTTTTAAAACTTCCAAGAAAGTGGATGACGTTGGTTGACATGGATACTGATAAGGAATACAAGTGCCAGCTCAACACCGCTGAACGGAACAAAGATGAAAAAGCTTTGGGAAGAGGATGGTCAAAATTTGTTAAGGATCGCCGCTTAAAAGAGGGTGACAAGCTAATTTTTGATTTGGATCGACCTCCTAGAGTTTTGTTTGTTCATGTGGTTCATAGGGGTAACATTGCTTGA

Protein sequence

>MS.gene50397.t1

MVNPDEERIQVDGDFFIRNQGRLQINKPGYARDGDCRGIVIQLGRDEFGFFMKNGAAIADKFELKAPTLAKFEYLGPLNMFLVTFLADQNRQVENNVELVPQVAANAHVVQEVVDNKKVVQDVIDISSDEEVDVTSDNEEQNQDVEEEEDPYHFEHKVSAAMSKRVLGQPMHFPNCVSREVLKLPRKWMTLVDMDTDKEYKCQLNTAERNKDEKALGRGWSKFVKDRRLKEGDKLIFDLDRPPRVLFVHVVHRGNIA