AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50413

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50413.t1 MTR_4g022370 97.260 365 9 1 1 365 1 364 0.0 721
MS.gene50413.t1 MTR_7g059400 49.871 387 123 15 1 361 1 342 3.53e-88 270
MS.gene50413.t1 MTR_4g063780 44.092 347 139 15 1 324 1 315 3.76e-64 207
MS.gene50413.t1 MTR_2g096740 40.244 328 112 17 1 309 1 263 4.43e-55 183
MS.gene50413.t1 MTR_2g096740 38.182 330 101 17 1 309 1 248 1.06e-47 163
MS.gene50413.t1 MTR_7g024670 36.504 389 189 15 1 355 1 365 2.25e-46 162
MS.gene50413.t1 MTR_3g091820 69.737 76 19 1 64 139 28 99 4.47e-32 122
MS.gene50413.t1 MTR_2g093220 80.328 61 12 0 77 137 34 94 4.61e-31 119
MS.gene50413.t1 MTR_8g027295 45.652 138 64 3 70 205 11 139 1.71e-30 117
MS.gene50413.t1 MTR_8g479350 51.351 111 49 2 83 189 68 177 7.46e-30 117
MS.gene50413.t1 MTR_5g031440 81.818 55 10 0 79 133 39 93 3.63e-29 115
MS.gene50413.t1 MTR_4g461080 65.385 78 26 1 58 135 5 81 3.75e-29 114
MS.gene50413.t1 MTR_8g015840 75.000 68 15 2 75 141 13 79 5.70e-29 112
MS.gene50413.t1 MTR_3g090430 64.865 74 26 0 64 137 32 105 7.78e-29 114
MS.gene50413.t1 MTR_3g077750 70.423 71 18 2 63 133 44 111 1.52e-28 114
MS.gene50413.t1 MTR_8g068210 69.841 63 19 0 80 142 29 91 1.94e-28 113
MS.gene50413.t1 MTR_8g079060 54.255 94 32 2 62 154 17 100 1.99e-28 110
MS.gene50413.t1 MTR_2g014170 86.275 51 7 0 82 132 44 94 3.12e-28 112
MS.gene50413.t1 MTR_2g059540 86.000 50 7 0 82 131 30 79 4.51e-27 106
MS.gene50413.t1 MTR_1g077600 55.000 80 34 1 60 137 18 97 7.77e-27 107
MS.gene50413.t1 MTR_1g056810 55.208 96 37 1 85 180 14 103 9.07e-27 108
MS.gene50413.t1 MTR_1g077600 55.000 80 34 1 60 137 18 97 1.03e-26 107
MS.gene50413.t1 MTR_4g109980 80.769 52 10 0 82 133 39 90 1.25e-26 108
MS.gene50413.t1 MTR_2g014060 61.905 84 22 3 51 134 3 76 3.19e-26 107
MS.gene50413.t1 MTR_4g089095 74.138 58 14 1 83 140 50 106 6.64e-26 105
MS.gene50413.t1 MTR_4g089095 74.138 58 14 1 83 140 16 72 7.98e-26 104
MS.gene50413.t1 MTR_8g044220 68.333 60 19 0 79 138 114 173 8.07e-26 107
MS.gene50413.t1 MTR_6g012450 67.213 61 20 0 79 139 132 192 2.22e-25 107
MS.gene50413.t1 MTR_2g013370 71.930 57 15 1 81 137 44 99 2.43e-25 103
MS.gene50413.t1 MTR_2g016030 70.175 57 17 0 80 136 44 100 6.40e-25 99.8
MS.gene50413.t1 MTR_6g027450 62.903 62 23 0 76 137 82 143 7.47e-25 103
MS.gene50413.t1 MTR_7g010950 62.500 64 24 0 76 139 128 191 1.22e-24 105
MS.gene50413.t1 MTR_4g082060 65.079 63 22 0 79 141 118 180 1.33e-24 104
MS.gene50413.t1 MTR_5g041420 41.121 107 57 1 33 139 94 194 3.34e-24 101
MS.gene50413.t1 MTR_7g086780 62.295 61 23 0 76 136 92 152 3.58e-24 103
MS.gene50413.t1 MTR_3g435480 63.492 63 23 0 79 141 132 194 4.78e-24 103
MS.gene50413.t1 MTR_6g027460 60.656 61 24 0 76 136 89 149 6.53e-24 101
MS.gene50413.t1 MTR_5g041380 53.247 77 36 0 67 143 111 187 1.00e-22 98.2
MS.gene50413.t1 MTR_5g041400 45.652 92 50 0 52 143 88 179 2.89e-22 97.1
MS.gene50413.t1 MTR_5g041530 43.299 97 45 2 43 136 104 193 1.28e-21 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50413.t1 AT3G55370 38.520 392 162 16 1 352 1 353 2.94e-56 187
MS.gene50413.t1 AT3G55370 43.878 294 109 12 1 277 1 255 4.69e-56 186
MS.gene50413.t1 AT3G55370 47.325 243 87 11 45 277 89 300 4.44e-49 169
MS.gene50413.t1 AT2G37590 41.349 341 133 13 1 310 1 305 6.30e-49 168
MS.gene50413.t1 AT5G02460 39.096 376 143 14 1 314 1 352 1.40e-46 163
MS.gene50413.t1 AT2G28810 34.501 371 176 16 1 344 1 331 7.55e-38 139
MS.gene50413.t1 AT1G07640 49.306 144 68 2 5 143 2 145 3.23e-37 137
MS.gene50413.t1 AT5G60200 58.333 108 44 1 79 185 51 158 7.94e-37 134
MS.gene50413.t1 AT1G07640 52.239 134 59 2 15 143 4 137 1.07e-36 135
MS.gene50413.t1 AT1G07640 70.886 79 23 0 65 143 3 81 4.34e-36 132
MS.gene50413.t1 AT2G28810 34.694 343 164 13 23 344 11 314 1.51e-35 132
MS.gene50413.t1 AT3G45610 55.238 105 44 2 79 182 36 138 4.06e-34 126
MS.gene50413.t1 AT5G65590 38.843 242 92 10 44 248 8 230 3.30e-32 123
MS.gene50413.t1 AT1G51700 61.053 95 33 3 78 168 27 121 4.38e-31 117
MS.gene50413.t1 AT1G28310 79.310 58 12 0 83 140 27 84 3.49e-30 117
MS.gene50413.t1 AT2G28510 81.967 61 11 0 82 142 47 107 4.09e-30 117
MS.gene50413.t1 AT1G28310 79.310 58 12 0 83 140 51 108 5.15e-30 117
MS.gene50413.t1 AT5G60850 71.014 69 20 0 70 138 38 106 1.67e-29 115
MS.gene50413.t1 AT3G61850 75.410 61 15 0 79 139 58 118 6.85e-29 113
MS.gene50413.t1 AT3G61850 75.410 61 15 0 79 139 58 118 6.85e-29 113
MS.gene50413.t1 AT3G61850 75.410 61 15 0 79 139 70 130 7.19e-29 114
MS.gene50413.t1 AT3G21270 84.906 53 8 0 83 135 29 81 8.25e-29 111
MS.gene50413.t1 AT3G61850 75.410 61 15 0 79 139 58 118 1.01e-28 114
MS.gene50413.t1 AT3G61850 75.410 61 15 0 79 139 70 130 1.30e-28 114
MS.gene50413.t1 AT4G38000 73.438 64 16 1 77 139 35 98 4.03e-28 110
MS.gene50413.t1 AT3G52440 58.140 86 36 0 80 165 22 107 4.81e-28 110
MS.gene50413.t1 AT3G52440 58.140 86 36 0 80 165 41 126 9.23e-28 110
MS.gene50413.t1 AT5G66940 48.958 96 38 2 64 148 2 97 3.48e-27 107
MS.gene50413.t1 AT1G21340 68.750 64 20 0 81 144 36 99 9.74e-27 107
MS.gene50413.t1 AT5G62940 86.000 50 7 0 84 133 74 123 1.79e-26 108
MS.gene50413.t1 AT3G50410 69.492 59 18 0 83 141 30 88 3.07e-26 105
MS.gene50413.t1 AT4G00940 71.186 59 17 0 79 137 64 122 5.39e-26 106
MS.gene50413.t1 AT4G00940 71.186 59 17 0 79 137 64 122 5.39e-26 106
MS.gene50413.t1 AT4G00940 71.186 59 17 0 79 137 64 122 5.39e-26 106
MS.gene50413.t1 AT2G34140 56.962 79 31 1 58 136 36 111 7.72e-26 102
MS.gene50413.t1 AT2G46590 78.846 52 11 0 79 130 64 115 2.05e-25 105
MS.gene50413.t1 AT2G46590 78.846 52 11 0 79 130 76 127 2.51e-25 105
MS.gene50413.t1 AT4G21050 66.129 62 21 0 76 137 17 78 5.22e-25 101
MS.gene50413.t1 AT1G29160 68.966 58 18 0 79 136 58 115 5.78e-25 100
MS.gene50413.t1 AT3G47500 64.062 64 23 0 76 139 103 166 9.79e-25 105
MS.gene50413.t1 AT1G64620 74.545 55 14 0 77 131 43 97 1.32e-24 103
MS.gene50413.t1 AT1G26790 55.072 69 31 0 68 136 96 164 3.75e-24 102
MS.gene50413.t1 AT5G62430 47.368 95 50 0 76 170 47 141 5.25e-24 100
MS.gene50413.t1 AT1G69570 62.295 61 23 0 76 136 125 185 5.58e-24 102
MS.gene50413.t1 AT5G39660 62.903 62 23 0 75 136 130 191 1.28e-23 102
MS.gene50413.t1 AT5G39660 62.903 62 23 0 75 136 130 191 1.28e-23 102
MS.gene50413.t1 AT5G39660 62.903 62 23 0 75 136 130 191 1.28e-23 102
MS.gene50413.t1 AT1G47655 68.333 60 17 1 74 131 18 77 1.85e-23 97.1
MS.gene50413.t1 AT4G21040 50.562 89 40 1 85 173 27 111 3.75e-22 94.0
MS.gene50413.t1 AT4G21080 47.674 86 41 1 85 170 27 108 7.31e-21 90.9
MS.gene50413.t1 AT4G21030 55.932 59 23 1 72 130 13 68 2.78e-14 71.2

Find 109 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGGAGGAGGAGGAGATTT+AGG 0.150054 4.4:-79354596 MS.gene50413:CDS
ATCAAGGAAGTGAACAATTT+TGG 0.154411 4.4:-79354229 MS.gene50413:CDS
AATTGGACTGATCATGTTTC+TGG 0.157222 4.4:-79354173 MS.gene50413:CDS
TCAACTTGCTTCTGTGAAAA+TGG 0.216358 4.4:-79354306 MS.gene50413:CDS
CCAGAAGTTGAAATCACTTT+TGG 0.251474 4.4:+79354338 None:intergenic
GCAAGTCTTGCAGAAGTGTC+TGG 0.259253 4.4:+79354892 None:intergenic
TCGGAGGAGGAGGAGATTTA+GGG 0.263782 4.4:-79354595 MS.gene50413:CDS
CCAAAAGTGATTTCAACTTC+TGG 0.271253 4.4:-79354338 MS.gene50413:CDS
GGTGATTTGGGAAGTGCTTT+AGG 0.279369 4.4:-79354539 MS.gene50413:CDS
GGAAGAGTGGATATTGTTAT+TGG 0.289267 4.4:+79354703 None:intergenic
ATGGGAGGAGTAGGTGATTT+GGG 0.291520 4.4:-79354551 MS.gene50413:CDS
CCTCCTCCTCCGAAATCATT+AGG 0.306462 4.4:+79354605 None:intergenic
GAGACGGAGCCGCCGTTTCC+TGG 0.321013 4.4:+79355130 None:intergenic
TTCAGTAACCCTAATGATTT+CGG 0.322960 4.4:-79354614 MS.gene50413:CDS
CATGGGAGGAGTAGGTGATT+TGG 0.324852 4.4:-79354552 MS.gene50413:CDS
AAATGGAAGATAGTATTAAT+AGG 0.328456 4.4:-79354289 MS.gene50413:CDS
TGGAATAGTGCTGTTGTTAC+TGG 0.330687 4.4:-79354209 MS.gene50413:CDS
CTGGTAATTCTGCAGCTAAT+TGG 0.337179 4.4:-79354190 MS.gene50413:CDS
GGACGAGAGGCGGGGCTCTA+AGG 0.342022 4.4:-79354859 MS.gene50413:CDS
TCCCGGTCGGTGGAGGCTTC+CGG 0.345043 4.4:-79354832 MS.gene50413:CDS
AATGGAAGATAGTATTAATA+GGG 0.346177 4.4:-79354288 MS.gene50413:CDS
GGAGATTTAGGGTTAAACTA+TGG 0.347109 4.4:-79354584 MS.gene50413:CDS
AATTAGCCAGAAAAGGAAAT+TGG 0.359477 4.4:+79354444 None:intergenic
CACTCTTCCGCCGATATATT+AGG 0.387275 4.4:-79354689 MS.gene50413:CDS
CTCCTCCTCCGAAATCATTA+GGG 0.390011 4.4:+79354606 None:intergenic
GCCCTATCGGACATCGATCC+TGG 0.393185 4.4:+79355019 None:intergenic
TGTGACTGTGGTGGTGGTGG+TGG 0.398669 4.4:+79355085 None:intergenic
TGCTTGATCTCTTGTTTCTC+CGG 0.410783 4.4:+79354813 None:intergenic
CTTCAAGTGGTTTGGAGCAA+TGG 0.413375 4.4:-79354499 MS.gene50413:CDS
TTGAACTAGCACTCCCCGTT+TGG 0.413567 4.4:+79354741 None:intergenic
CTTATCTTGATGCAGCCAAC+TGG 0.417162 4.4:-79355416 MS.gene50413:CDS
GCTAGCTCTGACCGCCAAAC+GGG 0.425727 4.4:-79354755 MS.gene50413:CDS
ACACTATAAAATTCTACATC+TGG 0.437923 4.4:+79354120 None:intergenic
GAAGGTACTGGACGAGAGGC+GGG 0.452725 4.4:-79354868 MS.gene50413:CDS
GTTTGCCATCCTTGCCCTAT+CGG 0.454338 4.4:+79355006 None:intergenic
GCAAGACTTGCAGAAGGTAC+TGG 0.454376 4.4:-79354880 MS.gene50413:CDS
CTCCGGAAGCCTCCACCGAC+CGG 0.457586 4.4:+79354830 None:intergenic
TGCAGCCAACTGGCAGCAAC+AGG 0.457644 4.4:-79355406 MS.gene50413:intron
GAGGATCCACCGGCTCAATT+AGG 0.467697 4.4:-79355042 MS.gene50413:CDS
CCCTCCAAATTAGCCAGAAA+AGG 0.471049 4.4:+79354437 None:intergenic
TTTGAACATTTCCTTCAAAA+GGG 0.473490 4.4:+79354396 None:intergenic
AGGAATGTCCCGGTCGGTGG+AGG 0.474332 4.4:-79354839 MS.gene50413:CDS
TTTAGTTACATGGGAGGAGT+AGG 0.477627 4.4:-79354560 MS.gene50413:CDS
AACTAGCACTCCCCGTTTGG+CGG 0.478302 4.4:+79354744 None:intergenic
AGCAACAATAACAGTAATCA+AGG 0.480984 4.4:-79354245 MS.gene50413:CDS
AGGTGTAAGGCCTAATATAT+CGG 0.483491 4.4:+79354679 None:intergenic
GGCTAGCTCTGACCGCCAAA+CGG 0.486093 4.4:-79354756 MS.gene50413:CDS
CCTAATGATTTCGGAGGAGG+AGG 0.502971 4.4:-79354605 MS.gene50413:CDS
TGAGATTGTGACTGTGGTGG+TGG 0.503091 4.4:+79355079 None:intergenic
CAAGTCTTGCAGAAGTGTCT+GGG 0.504581 4.4:+79354893 None:intergenic
GATTGTGACTGTGGTGGTGG+TGG 0.504773 4.4:+79355082 None:intergenic
AACCCTAATGATTTCGGAGG+AGG 0.505763 4.4:-79354608 MS.gene50413:CDS
AGAGTGGATATTGTTATTGG+AGG 0.506614 4.4:+79354706 None:intergenic
GGACATTTCAGTGCTGCCTC+CGG 0.520025 4.4:+79354974 None:intergenic
TTCAGTGCTGCCTCCGGCAT+AGG 0.523439 4.4:+79354980 None:intergenic
CTAAGGAATGTCCCGGTCGG+TGG 0.528029 4.4:-79354842 MS.gene50413:CDS
AACTATGGATTTAGTTACAT+GGG 0.529157 4.4:-79354569 MS.gene50413:CDS
GATGGCAAACATGCCTATGC+CGG 0.531496 4.4:-79354993 MS.gene50413:CDS
TTACTGAAGTTATGATGTAA+AGG 0.534579 4.4:+79354629 None:intergenic
ATCAAGATAAGCTGCTGGTA+TGG 0.536728 4.4:+79355428 None:intergenic
ATTTACCTGTTGCTGCCAGT+TGG 0.539661 4.4:+79355401 None:intergenic
CATCATCAGCAACCAGGAAA+CGG 0.545159 4.4:-79355142 MS.gene50413:CDS
TCTCAACCTCATGGAGGAGG+AGG 0.545906 4.4:-79355061 MS.gene50413:CDS
CACCTCAAATGTCATCATTG+AGG 0.546296 4.4:-79354661 MS.gene50413:CDS
AAACTATGGATTTAGTTACA+TGG 0.549318 4.4:-79354570 MS.gene50413:CDS
CGATCCTGGCCTAATTGAGC+CGG 0.550851 4.4:+79355033 None:intergenic
TGGAAACTCAACAAAGTCTC+CGG 0.552418 4.4:-79354777 MS.gene50413:CDS
CAAGTGGTTTGGAGCAATGG+AGG 0.557508 4.4:-79354496 MS.gene50413:CDS
ATGATGACATTTGAGGTGTA+AGG 0.560208 4.4:+79354666 None:intergenic
TCCGGAAGCCTCCACCGACC+GGG 0.560245 4.4:+79354831 None:intergenic
GCCAGGATCGATGTCCGATA+GGG 0.563470 4.4:-79355020 MS.gene50413:CDS
CAGTCACAATCTCAACCTCA+TGG 0.566499 4.4:-79355070 MS.gene50413:CDS
TCAAGCAAAAGTAGCAACAA+TGG 0.567762 4.4:-79354797 MS.gene50413:CDS
TCCTGGCCTAATTGAGCCGG+TGG 0.569726 4.4:+79355036 None:intergenic
CAGAATCATCATCAGCAACC+AGG 0.572861 4.4:-79355148 MS.gene50413:CDS
GATGTCCGATAGGGCAAGGA+TGG 0.575021 4.4:-79355011 MS.gene50413:CDS
AACCTCAATGATGACATTTG+AGG 0.576269 4.4:+79354659 None:intergenic
CTAATATATCGGCGGAAGAG+TGG 0.581651 4.4:+79354690 None:intergenic
ATGGAAGATAGTATTAATAG+GGG 0.583129 4.4:-79354287 MS.gene50413:CDS
TCCACCGGCTCAATTAGGCC+AGG 0.583672 4.4:-79355037 MS.gene50413:CDS
GATTTGGGAAGTGCTTTAGG+AGG 0.585086 4.4:-79354536 MS.gene50413:CDS
CATGGAGGAGGAGGATCCAC+CGG 0.586222 4.4:-79355052 MS.gene50413:CDS
GGCCAGGATCGATGTCCGAT+AGG 0.588624 4.4:-79355021 MS.gene50413:CDS
CAATCTCAACCTCATGGAGG+AGG 0.588847 4.4:-79355064 MS.gene50413:CDS
AGAAGGTACTGGACGAGAGG+CGG 0.590108 4.4:-79354869 MS.gene50413:CDS
CGGGGCTCTAAGGAATGTCC+CGG 0.591050 4.4:-79354849 MS.gene50413:CDS
GCTCTAAGGAATGTCCCGGT+CGG 0.592207 4.4:-79354845 MS.gene50413:CDS
CTGAAGCAGTTGTTGAGAGA+CGG 0.593094 4.4:+79355114 None:intergenic
GCTGCATCAAGATAAGCTGC+TGG 0.594629 4.4:+79355423 None:intergenic
GTTTGGCGGTCAGAGCTAGC+CGG 0.603255 4.4:+79354758 None:intergenic
AATCATGAAATGATAGCTAG+TGG 0.606984 4.4:-79354374 MS.gene50413:CDS
GATCGATGTCCGATAGGGCA+AGG 0.614474 4.4:-79355015 MS.gene50413:CDS
AGTAACCCTAATGATTTCGG+AGG 0.621947 4.4:-79354611 MS.gene50413:CDS
GGCAAACATGCCTATGCCGG+AGG 0.625436 4.4:-79354990 MS.gene50413:CDS
CTAGCTCTGACCGCCAAACG+GGG 0.632683 4.4:-79354754 MS.gene50413:CDS
CATGAAATGATAGCTAGTGG+TGG 0.634205 4.4:-79354371 MS.gene50413:CDS
TGTTGATGAGTCATTGACAT+TGG 0.643301 4.4:+79354470 None:intergenic
TGCAGAAGGTACTGGACGAG+AGG 0.643597 4.4:-79354872 MS.gene50413:CDS
TGAGGTTGAGATTGTGACTG+TGG 0.644332 4.4:+79355073 None:intergenic
GGTTGAGATTGTGACTGTGG+TGG 0.650158 4.4:+79355076 None:intergenic
CATCAGCAACCAGGAAACGG+CGG 0.659295 4.4:-79355139 MS.gene50413:CDS
TATGGATTTAGTTACATGGG+AGG 0.660158 4.4:-79354566 MS.gene50413:CDS
GTGGATCCTCCTCCTCCATG+AGG 0.661047 4.4:+79355055 None:intergenic
AAGGTACTGGACGAGAGGCG+GGG 0.664600 4.4:-79354867 MS.gene50413:CDS
ACTTCTGCAAGACTTGCAGA+AGG 0.679196 4.4:-79354886 MS.gene50413:CDS
TGTAAGGCCTAATATATCGG+CGG 0.708601 4.4:+79354682 None:intergenic
GTGTTGGTTGATTCACAACG+TGG 0.731087 4.4:+79354953 None:intergenic
TAGCTAGTGGTGGATATGTG+AGG 0.738159 4.4:-79354361 MS.gene50413:CDS
TCACAATCTCAACCTCATGG+AGG 0.766876 4.4:-79355067 MS.gene50413:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTCAAAAGATAAAA+AGG - chr4.4:79354257-79354276 MS.gene50413:CDS 10.0%
!! TAAGAAAGAAATCAAATATT+TGG + chr4.4:79354330-79354349 None:intergenic 15.0%
!!! TTTTGAATTATTTTTCCTTT+TGG + chr4.4:79354250-79354269 None:intergenic 15.0%
!! AAATGGAAGATAGTATTAAT+AGG - chr4.4:79355285-79355304 MS.gene50413:intron 20.0%
!! AATGGAAGATAGTATTAATA+GGG - chr4.4:79355286-79355305 MS.gene50413:intron 20.0%
!! ACAGGTAAATATAATTAGTA+AGG - chr4.4:79354186-79354205 MS.gene50413:CDS 20.0%
!! AGTATATGAATTGAGAATAA+TGG + chr4.4:79354364-79354383 None:intergenic 20.0%
!! CAGGTAAATATAATTAGTAA+GGG - chr4.4:79354187-79354206 MS.gene50413:CDS 20.0%
!!! AATTCAATTTTATCTTCAAG+TGG - chr4.4:79355062-79355081 MS.gene50413:CDS 20.0%
! AAACTATGGATTTAGTTACA+TGG - chr4.4:79355004-79355023 MS.gene50413:CDS 25.0%
! AACTATGGATTTAGTTACAT+GGG - chr4.4:79355005-79355024 MS.gene50413:CDS 25.0%
! ATGGAAGATAGTATTAATAG+GGG - chr4.4:79355287-79355306 MS.gene50413:intron 25.0%
! GTAAGGGTTTCAATTTAATT+TGG - chr4.4:79354203-79354222 MS.gene50413:CDS 25.0%
! TATTTGGTATATTCAAGAAG+AGG + chr4.4:79354314-79354333 None:intergenic 25.0%
! TGCTGAAATAACAAACAAAA+AGG + chr4.4:79354405-79354424 None:intergenic 25.0%
! TTACTGAAGTTATGATGTAA+AGG + chr4.4:79354948-79354967 None:intergenic 25.0%
! TTTGAACATTTCCTTCAAAA+GGG + chr4.4:79355181-79355200 None:intergenic 25.0%
!! AATTGAGAATAATGGAAGAA+AGG + chr4.4:79354356-79354375 None:intergenic 25.0%
!! AATTTTATCTTCAAGTGGTT+TGG - chr4.4:79355067-79355086 MS.gene50413:CDS 25.0%
!! TTTTGAACATTTCCTTCAAA+AGG + chr4.4:79355182-79355201 None:intergenic 25.0%
AATCATGAAATGATAGCTAG+TGG - chr4.4:79355200-79355219 MS.gene50413:intron 30.0%
AATTAGCCAGAAAAGGAAAT+TGG + chr4.4:79355133-79355152 None:intergenic 30.0%
AGCAACAATAACAGTAATCA+AGG - chr4.4:79355329-79355348 MS.gene50413:intron 30.0%
TTCAGTAACCCTAATGATTT+CGG - chr4.4:79354960-79354979 MS.gene50413:CDS 30.0%
! AGTATTAATAGGGGCTTTTT+GGG - chr4.4:79355296-79355315 MS.gene50413:intron 30.0%
! ATCAAGGAAGTGAACAATTT+TGG - chr4.4:79355345-79355364 MS.gene50413:intron 30.0%
! CTTTTGGACAAAGAAACAAA+AGG + chr4.4:79354234-79354253 None:intergenic 30.0%
! GAAGTAGCAAAATTTTGTGT+TGG + chr4.4:79354640-79354659 None:intergenic 30.0%
! TAGTATTAATAGGGGCTTTT+TGG - chr4.4:79355295-79355314 MS.gene50413:intron 30.0%
!! AGTAATTTGTACCCTTTTGA+AGG - chr4.4:79355167-79355186 MS.gene50413:intron 30.0%
!! CTTTTGTTTCTTTGTCCAAA+AGG - chr4.4:79354232-79354251 MS.gene50413:CDS 30.0%
!!! ATTTCCTTTTCTGGCTAATT+TGG - chr4.4:79355133-79355152 MS.gene50413:CDS 30.0%
AACCTCAATGATGACATTTG+AGG + chr4.4:79354918-79354937 None:intergenic 35.0%
AATTGGACTGATCATGTTTC+TGG - chr4.4:79355401-79355420 MS.gene50413:intron 35.0%
AGGTGTAAGGCCTAATATAT+CGG + chr4.4:79354898-79354917 None:intergenic 35.0%
CCAAAAGTGATTTCAACTTC+TGG - chr4.4:79355236-79355255 MS.gene50413:intron 35.0%
GGAAGAGTGGATATTGTTAT+TGG + chr4.4:79354874-79354893 None:intergenic 35.0%
TATGGATTTAGTTACATGGG+AGG - chr4.4:79355008-79355027 MS.gene50413:CDS 35.0%
TCAAGCAAAAGTAGCAACAA+TGG - chr4.4:79354777-79354796 MS.gene50413:CDS 35.0%
! AGAGTGGATATTGTTATTGG+AGG + chr4.4:79354871-79354890 None:intergenic 35.0%
! ATGATGACATTTGAGGTGTA+AGG + chr4.4:79354911-79354930 None:intergenic 35.0%
! CCAGAAGTTGAAATCACTTT+TGG + chr4.4:79355239-79355258 None:intergenic 35.0%
! GGAGATTTAGGGTTAAACTA+TGG - chr4.4:79354990-79355009 MS.gene50413:CDS 35.0%
! TCAACTTGCTTCTGTGAAAA+TGG - chr4.4:79355268-79355287 MS.gene50413:intron 35.0%
! TGTTGATGAGTCATTGACAT+TGG + chr4.4:79355107-79355126 None:intergenic 35.0%
AGTAACCCTAATGATTTCGG+AGG - chr4.4:79354963-79354982 MS.gene50413:CDS 40.0%
ATCAAGATAAGCTGCTGGTA+TGG + chr4.4:79354149-79354168 None:intergenic 40.0%
CACCTCAAATGTCATCATTG+AGG - chr4.4:79354913-79354932 MS.gene50413:CDS 40.0%
CATGAAATGATAGCTAGTGG+TGG - chr4.4:79355203-79355222 MS.gene50413:intron 40.0%
CTGGTAATTCTGCAGCTAAT+TGG - chr4.4:79355384-79355403 MS.gene50413:intron 40.0%
TGGAAACTCAACAAAGTCTC+CGG - chr4.4:79354797-79354816 MS.gene50413:CDS 40.0%
TGTAAGGCCTAATATATCGG+CGG + chr4.4:79354895-79354914 None:intergenic 40.0%
TTTAGTTACATGGGAGGAGT+AGG - chr4.4:79355014-79355033 MS.gene50413:CDS 40.0%
! ACAGCACCAATTTCCTTTTC+TGG - chr4.4:79355124-79355143 MS.gene50413:CDS 40.0%
! TGCTTGATCTCTTGTTTCTC+CGG + chr4.4:79354764-79354783 None:intergenic 40.0%
! TGGAATAGTGCTGTTGTTAC+TGG - chr4.4:79355365-79355384 MS.gene50413:intron 40.0%
!!! CCTTTTCTGGCTAATTTGGA+GGG - chr4.4:79355137-79355156 MS.gene50413:CDS 40.0%
!!! TCCTTTTCTGGCTAATTTGG+AGG - chr4.4:79355136-79355155 MS.gene50413:CDS 40.0%
AACCCTAATGATTTCGGAGG+AGG - chr4.4:79354966-79354985 MS.gene50413:CDS 45.0%
ACTTCTGCAAGACTTGCAGA+AGG - chr4.4:79354688-79354707 MS.gene50413:CDS 45.0%
ATGGGAGGAGTAGGTGATTT+GGG - chr4.4:79355023-79355042 MS.gene50413:CDS 45.0%
ATTTACCTGTTGCTGCCAGT+TGG + chr4.4:79354176-79354195 None:intergenic 45.0%
CACTCTTCCGCCGATATATT+AGG - chr4.4:79354885-79354904 MS.gene50413:CDS 45.0%
CAGAATCATCATCAGCAACC+AGG - chr4.4:79354426-79354445 MS.gene50413:CDS 45.0%
CAGTCACAATCTCAACCTCA+TGG - chr4.4:79354504-79354523 MS.gene50413:CDS 45.0%
CATCATCAGCAACCAGGAAA+CGG - chr4.4:79354432-79354451 MS.gene50413:CDS 45.0%
CCCTCCAAATTAGCCAGAAA+AGG + chr4.4:79355140-79355159 None:intergenic 45.0%
CTAATATATCGGCGGAAGAG+TGG + chr4.4:79354887-79354906 None:intergenic 45.0%
CTCCTCCTCCGAAATCATTA+GGG + chr4.4:79354971-79354990 None:intergenic 45.0%
CTGAAGCAGTTGTTGAGAGA+CGG + chr4.4:79354463-79354482 None:intergenic 45.0%
CTTATCTTGATGCAGCCAAC+TGG - chr4.4:79354158-79354177 MS.gene50413:CDS 45.0%
TCACAATCTCAACCTCATGG+AGG - chr4.4:79354507-79354526 MS.gene50413:CDS 45.0%
TGAGGTTGAGATTGTGACTG+TGG + chr4.4:79354504-79354523 None:intergenic 45.0%
! CTTCAAGTGGTTTGGAGCAA+TGG - chr4.4:79355075-79355094 MS.gene50413:CDS 45.0%
!! CAAGTCTTGCAGAAGTGTCT+GGG + chr4.4:79354684-79354703 None:intergenic 45.0%
!! GATTTGGGAAGTGCTTTAGG+AGG - chr4.4:79355038-79355057 MS.gene50413:CDS 45.0%
!! GGTGATTTGGGAAGTGCTTT+AGG - chr4.4:79355035-79355054 MS.gene50413:CDS 45.0%
!! GTGTTGGTTGATTCACAACG+TGG + chr4.4:79354624-79354643 None:intergenic 45.0%
!! TAGCTAGTGGTGGATATGTG+AGG - chr4.4:79355213-79355232 MS.gene50413:intron 45.0%
CAATCTCAACCTCATGGAGG+AGG - chr4.4:79354510-79354529 MS.gene50413:CDS 50.0%
CATGGGAGGAGTAGGTGATT+TGG - chr4.4:79355022-79355041 MS.gene50413:CDS 50.0%
CCTAATGATTTCGGAGGAGG+AGG - chr4.4:79354969-79354988 MS.gene50413:CDS 50.0%
CCTCCTCCTCCGAAATCATT+AGG + chr4.4:79354972-79354991 None:intergenic 50.0%
GATGGCAAACATGCCTATGC+CGG - chr4.4:79354581-79354600 MS.gene50413:CDS 50.0%
GCTGCATCAAGATAAGCTGC+TGG + chr4.4:79354154-79354173 None:intergenic 50.0%
GGTTGAGATTGTGACTGTGG+TGG + chr4.4:79354501-79354520 None:intergenic 50.0%
GTTTGCCATCCTTGCCCTAT+CGG + chr4.4:79354571-79354590 None:intergenic 50.0%
TCGGAGGAGGAGGAGATTTA+GGG - chr4.4:79354979-79354998 MS.gene50413:CDS 50.0%
TGAGATTGTGACTGTGGTGG+TGG + chr4.4:79354498-79354517 None:intergenic 50.0%
TTCGGAGGAGGAGGAGATTT+AGG - chr4.4:79354978-79354997 MS.gene50413:CDS 50.0%
TTGAACTAGCACTCCCCGTT+TGG + chr4.4:79354836-79354855 None:intergenic 50.0%
! CAAGTGGTTTGGAGCAATGG+AGG - chr4.4:79355078-79355097 MS.gene50413:CDS 50.0%
! GCAAGACTTGCAGAAGGTAC+TGG - chr4.4:79354694-79354713 MS.gene50413:CDS 50.0%
!! GCAAGTCTTGCAGAAGTGTC+TGG + chr4.4:79354685-79354704 None:intergenic 50.0%
AACTAGCACTCCCCGTTTGG+CGG + chr4.4:79354833-79354852 None:intergenic 55.0%
CATCAGCAACCAGGAAACGG+CGG - chr4.4:79354435-79354454 MS.gene50413:CDS 55.0%
CGATCCTGGCCTAATTGAGC+CGG + chr4.4:79354544-79354563 None:intergenic 55.0%
GATCGATGTCCGATAGGGCA+AGG - chr4.4:79354559-79354578 MS.gene50413:CDS 55.0%
GATGTCCGATAGGGCAAGGA+TGG - chr4.4:79354563-79354582 MS.gene50413:CDS 55.0%
GCCAGGATCGATGTCCGATA+GGG - chr4.4:79354554-79354573 MS.gene50413:CDS 55.0%
GGACATTTCAGTGCTGCCTC+CGG + chr4.4:79354603-79354622 None:intergenic 55.0%
TCTCAACCTCATGGAGGAGG+AGG - chr4.4:79354513-79354532 MS.gene50413:CDS 55.0%
!! AGAAGGTACTGGACGAGAGG+CGG - chr4.4:79354705-79354724 MS.gene50413:CDS 55.0%
!! GAGGATCCACCGGCTCAATT+AGG - chr4.4:79354532-79354551 MS.gene50413:CDS 55.0%
!! GATTGTGACTGTGGTGGTGG+TGG + chr4.4:79354495-79354514 None:intergenic 55.0%
!! GCTCTAAGGAATGTCCCGGT+CGG - chr4.4:79354729-79354748 MS.gene50413:CDS 55.0%
!! TGCAGAAGGTACTGGACGAG+AGG - chr4.4:79354702-79354721 MS.gene50413:CDS 55.0%
CATGGAGGAGGAGGATCCAC+CGG - chr4.4:79354522-79354541 MS.gene50413:CDS 60.0%
CTAAGGAATGTCCCGGTCGG+TGG - chr4.4:79354732-79354751 MS.gene50413:CDS 60.0%
GCCCTATCGGACATCGATCC+TGG + chr4.4:79354558-79354577 None:intergenic 60.0%
GGCAAACATGCCTATGCCGG+AGG - chr4.4:79354584-79354603 MS.gene50413:CDS 60.0%
GGCCAGGATCGATGTCCGAT+AGG - chr4.4:79354553-79354572 MS.gene50413:CDS 60.0%
GTGGATCCTCCTCCTCCATG+AGG + chr4.4:79354522-79354541 None:intergenic 60.0%
GTTTGGCGGTCAGAGCTAGC+CGG + chr4.4:79354819-79354838 None:intergenic 60.0%
TCCTGGCCTAATTGAGCCGG+TGG + chr4.4:79354541-79354560 None:intergenic 60.0%
TGCAGCCAACTGGCAGCAAC+AGG - chr4.4:79354168-79354187 MS.gene50413:CDS 60.0%
TTCAGTGCTGCCTCCGGCAT+AGG + chr4.4:79354597-79354616 None:intergenic 60.0%
!! AAGGTACTGGACGAGAGGCG+GGG - chr4.4:79354707-79354726 MS.gene50413:CDS 60.0%
!! CGGGGCTCTAAGGAATGTCC+CGG - chr4.4:79354725-79354744 MS.gene50413:CDS 60.0%
!! CTAGCTCTGACCGCCAAACG+GGG - chr4.4:79354820-79354839 MS.gene50413:CDS 60.0%
!! GAAGGTACTGGACGAGAGGC+GGG - chr4.4:79354706-79354725 MS.gene50413:CDS 60.0%
!! GCTAGCTCTGACCGCCAAAC+GGG - chr4.4:79354819-79354838 MS.gene50413:CDS 60.0%
!! GGCTAGCTCTGACCGCCAAA+CGG - chr4.4:79354818-79354837 MS.gene50413:CDS 60.0%
!! TCCACCGGCTCAATTAGGCC+AGG - chr4.4:79354537-79354556 MS.gene50413:CDS 60.0%
!! TGTGACTGTGGTGGTGGTGG+TGG + chr4.4:79354492-79354511 None:intergenic 60.0%
AGGAATGTCCCGGTCGGTGG+AGG - chr4.4:79354735-79354754 MS.gene50413:CDS 65.0%
!! GACTGTGGTGGTGGTGGTGG+TGG + chr4.4:79354489-79354508 None:intergenic 65.0%
CTCCGGAAGCCTCCACCGAC+CGG + chr4.4:79354747-79354766 None:intergenic 70.0%
GAGACGGAGCCGCCGTTTCC+TGG + chr4.4:79354447-79354466 None:intergenic 70.0%
GGACGAGAGGCGGGGCTCTA+AGG - chr4.4:79354715-79354734 MS.gene50413:CDS 70.0%
TCCCGGTCGGTGGAGGCTTC+CGG - chr4.4:79354742-79354761 MS.gene50413:CDS 70.0%
TCCGGAAGCCTCCACCGACC+GGG + chr4.4:79354746-79354765 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 79354133 79355463 79354133 ID=MS.gene50413
chr4.4 mRNA 79354133 79355463 79354133 ID=MS.gene50413.t1;Parent=MS.gene50413
chr4.4 exon 79355407 79355463 79355407 ID=MS.gene50413.t1.exon1;Parent=MS.gene50413.t1
chr4.4 CDS 79355407 79355463 79355407 ID=cds.MS.gene50413.t1;Parent=MS.gene50413.t1
chr4.4 exon 79354133 79355173 79354133 ID=MS.gene50413.t1.exon2;Parent=MS.gene50413.t1
chr4.4 CDS 79354133 79355173 79354133 ID=cds.MS.gene50413.t1;Parent=MS.gene50413.t1
Gene Sequence

>MS.gene50413

ATGGTTTTTACTTCCATACCAGCAGCTTATCTTGATGCAGCCAACTGGCAGCAACAGGTAAATATAATTAGTAAGGGTTTCAATTTAATTTGGATCTTCCTTTTGTTTCTTTGTCCAAAAGGAAAAATAATTCAAAAGATAAAAAGGAAAAACAATATTCTTGTTACTTTTATTTATACCTCTTCTTGAATATACCAAATATTTGATTTCTTTCTTATTACCTTTCTTCCATTATTCTCAATTCATATACTCTTTTTCTAATTTTTCTTCCTTTTTGTTTGTTATTTCAGCAACAGAATCATCATCAGCAACCAGGAAACGGCGGCTCCGTCTCTCAACAACTGCTTCAGACACCACCACCACCACCACCACAGTCACAATCTCAACCTCATGGAGGAGGAGGATCCACCGGCTCAATTAGGCCAGGATCGATGTCCGATAGGGCAAGGATGGCAAACATGCCTATGCCGGAGGCAGCACTGAAATGTCCACGTTGTGAATCAACCAACACAAAATTTTGCTACTTCAACAACTACAGTCTCTCTCAGCCCAGACACTTCTGCAAGACTTGCAGAAGGTACTGGACGAGAGGCGGGGCTCTAAGGAATGTCCCGGTCGGTGGAGGCTTCCGGAGAAACAAGAGATCAAGCAAAAGTAGCAACAATGGAAACTCAACAAAGTCTCCGGCTAGCTCTGACCGCCAAACGGGGAGTGCTAGTTCAACAAACTCAATCTCCTCCAATAACAATATCCACTCTTCCGCCGATATATTAGGCCTTACACCTCAAATGTCATCATTGAGGTTCATGACACCTTTACATCATAACTTCAGTAACCCTAATGATTTCGGAGGAGGAGGAGATTTAGGGTTAAACTATGGATTTAGTTACATGGGAGGAGTAGGTGATTTGGGAAGTGCTTTAGGAGGTAATTCAATTTTATCTTCAAGTGGTTTGGAGCAATGGAGGATGCCAATGTCAATGACTCATCAACAGCACCAATTTCCTTTTCTGGCTAATTTGGAGGGTTCAAATAGTAATTTGTACCCTTTTGAAGGAAATGTTCAAAATCATGAAATGATAGCTAGTGGTGGATATGTGAGGCCAAAAGTGATTTCAACTTCTGGAATTATGACTCAACTTGCTTCTGTGAAAATGGAAGATAGTATTAATAGGGGCTTTTTGGGAATTAGCACAAGCAACAATAACAGTAATCAAGGAAGTGAACAATTTTGGAATAGTGCTGTTGTTACTGGTAATTCTGCAGCTAATTGGACTGATCATGTTTCTGGTTTTAGCTCTTCTTCTAATACTACTAGTAACCAGATGTAG
Protein sequence

>MS.gene50413.t1

MVFTSIPAAYLDAANWQQQQQNHHQQPGNGGSVSQQLLQTPPPPPPQSQSQPHGGGGSTGSIRPGSMSDRARMANMPMPEAALKCPRCESTNTKFCYFNNYSLSQPRHFCKTCRRYWTRGGALRNVPVGGGFRRNKRSSKSSNNGNSTKSPASSDRQTGSASSTNSISSNNNIHSSADILGLTPQMSSLRFMTPLHHNFSNPNDFGGGGDLGLNYGFSYMGGVGDLGSALGGNSILSSSGLEQWRMPMSMTHQQHQFPFLANLEGSNSNLYPFEGNVQNHEMIASGGYVRPKVISTSGIMTQLASVKMEDSINRGFLGISTSNNNSNQGSEQFWNSAVVTGNSAANWTDHVSGFSSSSNTTSNQM