AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50431

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50431.t1 MTR_4g021760 83.439 314 49 2 1 313 1 312 0.0 536
MS.gene50431.t1 MTR_4g021855 66.447 304 90 5 1 295 1 301 7.58e-138 392
MS.gene50431.t1 MTR_4g021845 84.475 219 32 1 1 219 1 217 4.49e-135 382
MS.gene50431.t1 MTR_4g021790 74.793 242 59 1 1 242 1 240 4.29e-126 361
MS.gene50431.t1 MTR_5g055260 59.740 231 64 5 1 231 1 202 1.65e-85 258
MS.gene50431.t1 MTR_4g023980 76.978 139 30 1 55 193 35 171 1.60e-71 219
MS.gene50431.t1 MTR_1g032570 37.534 373 166 9 2 309 3 373 3.74e-69 226
MS.gene50431.t1 MTR_6g045327 46.416 293 138 8 10 296 14 293 5.05e-69 220
MS.gene50431.t1 MTR_3g086100 40.316 253 118 5 10 231 13 263 2.82e-58 196
MS.gene50431.t1 MTR_8g079940 39.695 262 125 6 9 239 24 283 2.78e-56 191
MS.gene50431.t1 MTR_2g034960 39.245 265 133 4 10 248 29 291 8.87e-55 188
MS.gene50431.t1 MTR_4g121020 38.868 265 133 4 10 248 31 292 1.03e-54 189
MS.gene50431.t1 MTR_4g121020 38.868 265 133 4 10 248 31 292 1.09e-54 189
MS.gene50431.t1 MTR_8g105600 39.759 249 126 4 4 231 3 248 7.86e-52 179
MS.gene50431.t1 MTR_1g013170 39.200 250 118 4 7 226 2 247 1.28e-50 175
MS.gene50431.t1 MTR_7g117705 36.466 266 141 4 7 244 2 267 1.53e-49 171
MS.gene50431.t1 MTR_4g131600 36.842 247 135 4 9 234 19 265 1.08e-48 171
MS.gene50431.t1 MTR_8g093040 42.797 236 112 6 1 231 1 218 2.81e-48 163
MS.gene50431.t1 MTR_3g106220 37.652 247 124 3 10 226 8 254 6.51e-47 167
MS.gene50431.t1 MTR_1g013180 36.992 246 129 3 7 226 153 398 2.61e-45 163
MS.gene50431.t1 MTR_1g032570 34.314 306 135 8 68 309 1 304 1.87e-44 159
MS.gene50431.t1 MTR_4g131570 32.800 250 142 4 10 233 17 266 1.01e-41 152
MS.gene50431.t1 MTR_4g131580 33.871 248 141 4 9 233 16 263 1.21e-41 152
MS.gene50431.t1 MTR_2g450070 33.333 255 135 3 10 229 18 272 1.94e-41 152
MS.gene50431.t1 MTR_2g450070 33.333 255 135 3 10 229 18 272 3.08e-41 152
MS.gene50431.t1 MTR_3g102590 38.140 215 104 4 10 222 8 195 1.19e-40 142
MS.gene50431.t1 MTR_3g086100 39.691 194 85 4 68 231 1 192 8.69e-40 145
MS.gene50431.t1 MTR_3g102600 36.564 227 104 4 1 226 1 188 4.83e-39 137
MS.gene50431.t1 MTR_6g016850 42.857 161 86 3 4 161 3 160 8.34e-39 135
MS.gene50431.t1 MTR_3g102590 37.264 212 104 4 10 219 8 192 3.99e-38 134
MS.gene50431.t1 MTR_3g102600 35.135 222 104 4 1 221 1 183 1.59e-36 130
MS.gene50431.t1 MTR_7g026400 33.010 206 127 3 52 248 30 233 8.64e-32 125
MS.gene50431.t1 MTR_3g102600 35.838 173 74 2 54 226 4 139 1.04e-31 117
MS.gene50431.t1 MTR_0450s0040 29.508 244 161 4 12 248 6 245 3.10e-31 120
MS.gene50431.t1 MTR_3g102600 33.929 168 74 2 54 221 4 134 4.06e-29 108
MS.gene50431.t1 MTR_4g121020 36.559 186 90 3 88 248 6 188 1.10e-28 115
MS.gene50431.t1 MTR_8g032710 22.969 357 215 9 12 309 19 374 7.83e-21 92.8
MS.gene50431.t1 MTR_1g013160 34.211 152 67 3 71 193 1 148 1.21e-19 84.3
MS.gene50431.t1 MTR_2g090675 38.710 93 55 1 136 226 125 217 4.69e-14 72.8
MS.gene50431.t1 MTR_5g041350 55.556 54 23 1 170 222 15 68 1.23e-13 70.9
MS.gene50431.t1 MTR_6g032990 44.872 78 42 1 170 246 42 119 3.66e-13 69.7
MS.gene50431.t1 MTR_7g098250 40.594 101 55 3 161 256 253 353 3.71e-13 70.1
MS.gene50431.t1 MTR_4g078810 48.276 58 30 0 169 226 157 214 6.17e-13 69.3
MS.gene50431.t1 MTR_7g088070 40.659 91 51 2 168 257 48 136 8.02e-13 67.8
MS.gene50431.t1 MTR_4g081710 51.852 54 25 1 170 222 17 70 1.49e-12 67.8
MS.gene50431.t1 MTR_2g086450 47.887 71 35 2 153 222 29 98 1.72e-12 67.8
MS.gene50431.t1 MTR_7g088070 51.786 56 26 1 168 222 48 103 2.54e-12 66.2
MS.gene50431.t1 MTR_5g014040 34.940 83 54 0 169 251 310 392 3.58e-12 67.4
MS.gene50431.t1 MTR_7g115530 47.222 72 36 2 170 239 218 289 4.71e-12 65.9
MS.gene50431.t1 MTR_7g115530 40.659 91 51 3 170 258 218 307 6.46e-12 65.9
MS.gene50431.t1 MTR_2g027860 55.556 54 23 1 170 222 49 102 1.22e-11 65.1
MS.gene50431.t1 MTR_7g115530 50.000 64 30 2 170 231 218 281 1.22e-11 65.1
MS.gene50431.t1 MTR_7g115530 40.659 91 51 3 170 258 218 307 1.46e-11 65.1
MS.gene50431.t1 MTR_7g089010 48.276 58 30 0 169 226 90 147 1.50e-11 64.3
MS.gene50431.t1 MTR_4g098870 48.148 54 28 0 169 222 309 362 1.67e-11 65.1
MS.gene50431.t1 MTR_4g098870 48.148 54 28 0 169 222 309 362 1.74e-11 65.1
MS.gene50431.t1 MTR_4g098870 48.148 54 28 0 169 222 329 382 1.86e-11 65.1
MS.gene50431.t1 MTR_4g098870 48.148 54 28 0 169 222 329 382 1.94e-11 65.1
MS.gene50431.t1 MTR_4g098870 48.148 54 28 0 169 222 348 401 1.97e-11 65.1
MS.gene50431.t1 MTR_1g080330 42.857 84 45 3 161 242 165 247 2.55e-11 63.5
MS.gene50431.t1 MTR_4g064730 46.552 58 31 0 169 226 130 187 3.30e-11 63.5
MS.gene50431.t1 MTR_7g093030 42.466 73 40 2 167 238 209 280 3.40e-11 63.2
MS.gene50431.t1 MTR_1g053835 42.254 71 38 2 153 222 243 311 3.71e-11 63.9
MS.gene50431.t1 MTR_1g080330 47.059 68 34 2 166 231 171 238 6.82e-11 63.2
MS.gene50431.t1 MTR_7g093030 47.368 57 29 1 167 222 209 265 7.88e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50431.t1 AT2G01760 39.382 259 124 5 7 232 4 262 4.31e-55 184
MS.gene50431.t1 AT1G67710 39.700 267 125 9 11 243 8 272 1.14e-53 183
MS.gene50431.t1 AT3G16857 36.039 308 139 9 10 259 33 340 1.45e-50 177
MS.gene50431.t1 AT3G16857 36.039 308 139 9 10 259 33 340 1.60e-50 177
MS.gene50431.t1 AT2G01760 35.540 287 124 6 7 232 4 290 3.65e-49 169
MS.gene50431.t1 AT4G31920 36.481 233 138 2 10 232 13 245 5.09e-46 163
MS.gene50431.t1 AT2G25180 38.750 240 123 6 10 226 13 251 6.68e-44 158
MS.gene50431.t1 AT3G16857 34.944 269 117 9 49 259 2 270 8.83e-40 147
MS.gene50431.t1 AT5G58080 37.349 249 132 6 10 236 14 260 5.33e-39 145
MS.gene50431.t1 AT1G67710 39.713 209 91 8 68 243 1 207 4.65e-36 135
MS.gene50431.t1 AT2G01760 37.056 197 92 4 68 232 1 197 4.86e-35 129
MS.gene50431.t1 AT5G49240 31.356 236 137 5 9 219 37 272 4.62e-34 126
MS.gene50431.t1 AT3G62670 30.258 271 159 9 28 272 53 319 2.77e-32 124
MS.gene50431.t1 AT1G49190 27.734 256 154 6 10 234 30 285 1.49e-28 114
MS.gene50431.t1 AT1G67710 38.095 189 82 8 88 243 12 198 5.04e-27 110
MS.gene50431.t1 AT5G07210 27.778 270 141 7 14 231 16 283 1.54e-25 107
MS.gene50431.t1 AT5G07210 26.978 278 141 8 14 231 16 291 5.08e-23 99.8
MS.gene50431.t1 AT2G27070 25.379 264 140 8 14 222 16 277 2.52e-19 89.0
MS.gene50431.t1 AT1G49190 32.258 124 83 1 10 132 30 153 1.72e-17 83.6
MS.gene50431.t1 AT5G29000 44.086 93 47 3 148 238 212 301 2.96e-15 75.5
MS.gene50431.t1 AT5G29000 42.000 100 53 3 148 245 212 308 3.28e-15 76.3
MS.gene50431.t1 AT5G29000 42.000 100 53 3 148 245 169 265 4.19e-15 75.5
MS.gene50431.t1 AT5G29000 42.000 100 53 3 148 245 169 265 4.19e-15 75.5
MS.gene50431.t1 AT5G44190 48.333 60 31 0 167 226 144 203 1.18e-13 71.2
MS.gene50431.t1 AT5G44190 48.333 60 31 0 167 226 144 203 1.38e-13 70.9
MS.gene50431.t1 AT3G04030 55.556 54 23 1 170 222 45 98 3.55e-13 70.1
MS.gene50431.t1 AT3G04030 55.556 54 23 1 170 222 45 98 4.01e-13 69.7
MS.gene50431.t1 AT4G28610 47.126 87 40 4 170 252 225 309 6.38e-13 69.3
MS.gene50431.t1 AT5G18240 55.556 54 23 1 170 222 45 98 6.97e-13 68.9
MS.gene50431.t1 AT5G18240 55.556 54 23 1 170 222 45 98 6.97e-13 68.9
MS.gene50431.t1 AT5G18240 55.556 54 23 1 170 222 45 98 7.97e-13 68.9
MS.gene50431.t1 AT5G18240 55.556 54 23 1 170 222 45 98 8.24e-13 68.9
MS.gene50431.t1 AT5G18240 55.556 54 23 1 170 222 45 98 8.24e-13 68.9
MS.gene50431.t1 AT2G01060 45.783 83 42 2 159 238 4 86 1.14e-12 67.8
MS.gene50431.t1 AT5G59570 42.105 76 41 1 151 226 125 197 1.18e-12 67.8
MS.gene50431.t1 AT5G59570 42.105 76 41 1 151 226 125 197 1.18e-12 67.8
MS.gene50431.t1 AT3G04450 48.276 58 29 1 166 222 234 291 1.62e-12 67.4
MS.gene50431.t1 AT3G46640 43.210 81 43 2 146 226 123 200 2.29e-12 67.0
MS.gene50431.t1 AT3G46640 43.210 81 43 2 146 226 123 200 2.29e-12 67.0
MS.gene50431.t1 AT3G04030 52.830 53 25 0 170 222 45 97 2.32e-12 67.4
MS.gene50431.t1 AT3G46640 43.210 81 43 2 146 226 123 200 2.53e-12 67.0
MS.gene50431.t1 AT3G04450 48.276 58 29 1 166 222 234 291 3.33e-12 67.0
MS.gene50431.t1 AT3G04450 48.276 58 29 1 166 222 194 251 4.09e-12 66.6
MS.gene50431.t1 AT3G04450 48.276 58 29 1 166 222 194 251 4.09e-12 66.6
MS.gene50431.t1 AT2G40970 38.158 76 47 0 151 226 86 161 5.87e-12 65.1
MS.gene50431.t1 AT1G69580 51.852 54 25 1 170 222 34 87 5.93e-12 65.9
MS.gene50431.t1 AT2G20570 46.552 58 31 0 169 226 152 209 6.42e-12 66.2
MS.gene50431.t1 AT1G69580 51.852 54 25 1 170 222 34 87 6.66e-12 65.9
MS.gene50431.t1 AT3G10760 48.276 58 30 0 169 226 104 161 1.07e-11 65.1
MS.gene50431.t1 AT5G05090 48.276 58 30 0 169 226 80 137 1.28e-11 64.3
MS.gene50431.t1 AT2G20570 47.273 55 29 0 172 226 171 225 4.18e-11 63.9
MS.gene50431.t1 AT4G18020 36.842 76 45 1 152 227 281 353 4.37e-11 63.9
MS.gene50431.t1 AT4G18020 36.842 76 45 1 152 227 281 353 4.37e-11 63.9
MS.gene50431.t1 AT4G18020 36.842 76 45 1 152 227 281 353 4.37e-11 63.9
MS.gene50431.t1 AT4G18020 36.842 76 45 1 152 227 281 353 4.37e-11 63.9
MS.gene50431.t1 AT4G18020 36.842 76 45 1 152 227 281 353 4.37e-11 63.9
MS.gene50431.t1 AT4G18020 36.842 76 45 1 152 227 281 353 4.37e-11 63.9
MS.gene50431.t1 AT4G18020 41.791 67 38 1 161 227 288 353 4.94e-11 63.9
MS.gene50431.t1 AT4G18020 41.791 67 38 1 161 227 288 353 4.94e-11 63.9
MS.gene50431.t1 AT4G18020 41.791 67 38 1 161 227 288 353 4.94e-11 63.9
MS.gene50431.t1 AT4G18020 41.791 67 38 1 161 227 288 353 4.94e-11 63.9
MS.gene50431.t1 AT4G18020 41.791 67 38 1 161 227 288 353 4.94e-11 63.9
MS.gene50431.t1 AT3G24120 48.529 68 31 2 170 233 41 108 6.47e-11 62.8
MS.gene50431.t1 AT3G24120 48.529 68 31 2 170 233 41 108 6.52e-11 62.4
MS.gene50431.t1 AT4G04605 46.970 66 33 2 168 231 12 77 9.02e-11 61.2

Find 60 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATTGTGTCGCAGAATTC+AGG 0.268438 4.4:-79695472 None:intergenic
ACATATTGCCAGCCATTTAC+AGG 0.282271 4.4:+79694966 MS.gene50431:CDS
TTCAGTTACCAAATGTAGTA+TGG 0.293347 4.4:+79694241 MS.gene50431:intron
CAACGACATTGGGTGGAAAT+TGG 0.345706 4.4:+79695512 MS.gene50431:CDS
TGAGTTGTAGGGTAACATTT+TGG 0.376494 4.4:-79694358 None:intergenic
GATAAATCATCATCAACATA+TGG 0.380393 4.4:-79695584 None:intergenic
AATGTGGGGCTTGCGAATAT+TGG 0.385194 4.4:+79694554 MS.gene50431:CDS
CGAATATTGGACTAAGCCTT+TGG 0.392176 4.4:+79694567 MS.gene50431:CDS
TGACAGGAATGTTGAGTTGT+AGG 0.408636 4.4:-79694370 None:intergenic
ATCAGAAGCCATACTACATT+TGG 0.409595 4.4:-79694249 None:intergenic
GGTTCAAGAAAATACAAAAC+GGG 0.418901 4.4:+79694684 MS.gene50431:CDS
CTTTCTTTCCAACATGTTGA+AGG 0.419409 4.4:-79694273 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.435563 4.4:+79694743 MS.gene50431:CDS
AAAAGAAACACTTGCAAAGA+AGG 0.435703 4.4:+79694729 MS.gene50431:CDS
GCAATAACCTCGAGACCGGC+TGG 0.445702 4.4:-79694054 None:intergenic
TTGCGATGCACAACGACATT+GGG 0.452490 4.4:+79695502 MS.gene50431:CDS
TCGAGACCGGCTGGGAATGA+TGG 0.453544 4.4:-79694045 None:intergenic
CTTCAACATGTTGGAAAGAA+AGG 0.459586 4.4:+79694274 MS.gene50431:CDS
AATAAATTCTTACTAATGAC+AGG 0.461511 4.4:-79694386 None:intergenic
AATAAAGAATATGAAAATGT+TGG 0.463084 4.4:+79694652 MS.gene50431:CDS
TTCTCGTGACCAAGACAAAC+GGG 0.467708 4.4:-79694752 None:intergenic
TCAACATATGGGAACAAGTT+GGG 0.468173 4.4:-79695572 None:intergenic
TGATGGGTTATCAGTGAAAC+GGG 0.468852 4.4:-79694028 None:intergenic
TACTCACTCAATGCCGGAGC+AGG 0.473606 4.4:+79695436 MS.gene50431:CDS
AAAAGTGTTGCTCATAATGT+TGG 0.490442 4.4:-79695540 None:intergenic
AAGTCATAAACATCCATGTC+TGG 0.491105 4.4:-79694332 None:intergenic
CACTTTCCTCAACTCTATTG+AGG 0.492388 4.4:+79694092 MS.gene50431:CDS
TGGTTCAAGAAAATACAAAA+CGG 0.498426 4.4:+79694683 MS.gene50431:CDS
AGGAAAGAATCATACTATCA+AGG 0.510494 4.4:-79694813 None:intergenic
ATTGCGATGCACAACGACAT+TGG 0.516098 4.4:+79695501 MS.gene50431:CDS
ATGATGGGTTATCAGTGAAA+CGG 0.516952 4.4:-79694029 None:intergenic
TGTAATCGATATCATTATCA+AGG 0.517908 4.4:+79694120 MS.gene50431:CDS
GACAGGAATGTTGAGTTGTA+GGG 0.519779 4.4:-79694369 None:intergenic
ATCATGAAGTCTATTGAATG+TGG 0.526495 4.4:+79694538 MS.gene50431:CDS
ATTCTCGTGACCAAGACAAA+CGG 0.526657 4.4:-79694753 None:intergenic
AAAATGAACCTGTAAATGGC+TGG 0.527802 4.4:-79694974 None:intergenic
TTCTGATGCCTTCAACATGT+TGG 0.527887 4.4:+79694265 MS.gene50431:CDS
CATGTTGCAAGAATTAGAAA+CGG 0.528421 4.4:+79694619 MS.gene50431:CDS
CGAGACCGGCTGGGAATGAT+GGG 0.533761 4.4:-79694044 None:intergenic
ATATGAAAATGTTGGAAGCT+CGG 0.535763 4.4:+79694660 MS.gene50431:CDS
CTACGTGCAGTCAATCAACT+AGG 0.547867 4.4:+79694790 MS.gene50431:CDS
ATAAATCATCATCAACATAT+GGG 0.548211 4.4:-79695583 None:intergenic
AGCTCAACAGAAGAGACAAA+AGG 0.548661 4.4:+79695359 MS.gene50431:CDS
AGAAAACTCTGGTCCTGCTC+CGG 0.548712 4.4:-79695449 None:intergenic
TCATGAAGTCTATTGAATGT+GGG 0.549366 4.4:+79694539 MS.gene50431:CDS
AATTGCAATAACCTCGAGAC+CGG 0.556151 4.4:-79694058 None:intergenic
ATCAACATATGGGAACAAGT+TGG 0.563419 4.4:-79695573 None:intergenic
CGATGCACAACGACATTGGG+TGG 0.580466 4.4:+79695505 MS.gene50431:CDS
AGAAGCTACTCACTCAATGC+CGG 0.591913 4.4:+79695430 MS.gene50431:CDS
ATCATTCCCAGCCGGTCTCG+AGG 0.599280 4.4:+79694047 MS.gene50431:CDS
CAATAACCTCGAGACCGGCT+GGG 0.602751 4.4:-79694053 None:intergenic
CAGAATTCAGGAGAAAACTC+TGG 0.602905 4.4:-79695460 None:intergenic
CATGAAGTCTATTGAATGTG+GGG 0.606058 4.4:+79694540 MS.gene50431:CDS
CAACAGAAGAGACAAAAGGT+AGG 0.617016 4.4:+79695363 MS.gene50431:CDS
ACATCTCCTCAATAGAGTTG+AGG 0.625514 4.4:-79694098 None:intergenic
TGATGCTCATATGCCAGACA+TGG 0.629107 4.4:+79694319 MS.gene50431:CDS
GATAACCCATCATTCCCAGC+CGG 0.638659 4.4:+79694039 MS.gene50431:CDS
TGAAAATGTTGGAAGCTCGG+TGG 0.688521 4.4:+79694663 MS.gene50431:CDS
ATGATTTATCATCACTTGTG+TGG 0.696325 4.4:+79695597 MS.gene50431:CDS
GTTCAAGAAAATACAAAACG+GGG 0.749177 4.4:+79694685 MS.gene50431:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATATGAAAATGT+TGG + chr4.4:79694652-79694671 MS.gene50431:CDS 15.0%
!!! AATATGTTCTTTTTTCATAT+CGG - chr4.4:79694953-79694972 None:intergenic 15.0%
!!! TGATTTTAAACTGAAATTTA+TGG + chr4.4:79695274-79695293 MS.gene50431:intron 15.0%
!! AAAAGATGAAAACTTGAATA+AGG - chr4.4:79694163-79694182 None:intergenic 20.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4.4:79694389-79694408 None:intergenic 20.0%
!! AGCATAGTAAAAAAGTAATT+AGG - chr4.4:79694836-79694855 None:intergenic 20.0%
!! ATAAATCATCATCAACATAT+GGG - chr4.4:79695586-79695605 None:intergenic 20.0%
!!! AAAAAAGATTCTGAAAATCA+TGG + chr4.4:79694921-79694940 MS.gene50431:CDS 20.0%
!!! ATATGTTCTTTTTTCATATC+GGG - chr4.4:79694952-79694971 None:intergenic 20.0%
!!! ATATTTCTTTTTTGTAGTGA+TGG + chr4.4:79694492-79694511 MS.gene50431:intron 20.0%
!!! ATGATTTTCAGAATCTTTTT+TGG - chr4.4:79694922-79694941 None:intergenic 20.0%
!!! ATGTTTTTCTCTTAATTTTC+AGG + chr4.4:79694889-79694908 MS.gene50431:intron 20.0%
!!! ATTCAAGTTTTCATCTTTTT+TGG + chr4.4:79694164-79694183 MS.gene50431:intron 20.0%
!!! TTTTGCAGAAATATAGAAAT+CGG + chr4.4:79695330-79695349 MS.gene50431:intron 20.0%
! AGTAGTATATTATCAACAAG+AGG - chr4.4:79695243-79695262 None:intergenic 25.0%
! GATAAATCATCATCAACATA+TGG - chr4.4:79695587-79695606 None:intergenic 25.0%
! TACAAAAATGAACCTGTAAA+TGG - chr4.4:79694981-79695000 None:intergenic 25.0%
! TACTTTACAACTTCAATAGT+TGG - chr4.4:79695139-79695158 None:intergenic 25.0%
! TGGTTCAAGAAAATACAAAA+CGG + chr4.4:79694683-79694702 MS.gene50431:CDS 25.0%
! TGTAATCGATATCATTATCA+AGG + chr4.4:79694120-79694139 MS.gene50431:CDS 25.0%
!!! AAGTCAAATTTTGTTTCAAG+AGG - chr4.4:79695410-79695429 None:intergenic 25.0%
AAAAGAAACACTTGCAAAGA+AGG + chr4.4:79694729-79694748 MS.gene50431:CDS 30.0%
AAAAGATAACTGACAAACCT+AGG - chr4.4:79695057-79695076 None:intergenic 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4.4:79694816-79694835 None:intergenic 30.0%
ATATGAAAATGTTGGAAGCT+CGG + chr4.4:79694660-79694679 MS.gene50431:CDS 30.0%
ATCATGAAGTCTATTGAATG+TGG + chr4.4:79694538-79694557 MS.gene50431:CDS 30.0%
ATGATTTATCATCACTTGTG+TGG + chr4.4:79695597-79695616 MS.gene50431:CDS 30.0%
CATGTTGCAAGAATTAGAAA+CGG + chr4.4:79694619-79694638 MS.gene50431:CDS 30.0%
GGTTCAAGAAAATACAAAAC+GGG + chr4.4:79694684-79694703 MS.gene50431:CDS 30.0%
GTTCAAGAAAATACAAAACG+GGG + chr4.4:79694685-79694704 MS.gene50431:CDS 30.0%
TCATGAAGTCTATTGAATGT+GGG + chr4.4:79694539-79694558 MS.gene50431:CDS 30.0%
TTCAGTTACCAAATGTAGTA+TGG + chr4.4:79694241-79694260 MS.gene50431:intron 30.0%
! CTTCATGATAGAACTTTTTG+TGG - chr4.4:79694527-79694546 None:intergenic 30.0%
! TTATCTTTTACCTAAGTCTG+TGG + chr4.4:79695068-79695087 MS.gene50431:intron 30.0%
!! AAAAGTGTTGCTCATAATGT+TGG - chr4.4:79695543-79695562 None:intergenic 30.0%
AAAAAGAGGTCAAGATCATG+TGG + chr4.4:79694593-79694612 MS.gene50431:CDS 35.0%
AAAATGAACCTGTAAATGGC+TGG - chr4.4:79694977-79694996 None:intergenic 35.0%
AAGTCATAAACATCCATGTC+TGG - chr4.4:79694335-79694354 None:intergenic 35.0%
AGAGGATGTATAGAAGCTAA+TGG - chr4.4:79695225-79695244 None:intergenic 35.0%
AGTAGTGAATTATGCAGTCA+TGG + chr4.4:79695155-79695174 MS.gene50431:intron 35.0%
ATCAACATATGGGAACAAGT+TGG - chr4.4:79695576-79695595 None:intergenic 35.0%
ATCAGAAGCCATACTACATT+TGG - chr4.4:79694252-79694271 None:intergenic 35.0%
CATGAAGTCTATTGAATGTG+GGG + chr4.4:79694540-79694559 MS.gene50431:CDS 35.0%
CTTCAACATGTTGGAAAGAA+AGG + chr4.4:79694274-79694293 MS.gene50431:CDS 35.0%
CTTTCTTTCCAACATGTTGA+AGG - chr4.4:79694276-79694295 None:intergenic 35.0%
TAAGCCTTTGGTTGAAAAAG+AGG + chr4.4:79694579-79694598 MS.gene50431:CDS 35.0%
TCAACATATGGGAACAAGTT+GGG - chr4.4:79695575-79695594 None:intergenic 35.0%
TGATTTACAACCACAGACTT+AGG - chr4.4:79695081-79695100 None:intergenic 35.0%
TGGAATAACATGCAAGACTA+AGG - chr4.4:79695119-79695138 None:intergenic 35.0%
! CTTTGCAAGTGTTTCTTTTG+AGG - chr4.4:79694728-79694747 None:intergenic 35.0%
! TGAGTTGTAGGGTAACATTT+TGG - chr4.4:79694361-79694380 None:intergenic 35.0%
!! ATGATGGGTTATCAGTGAAA+CGG - chr4.4:79694032-79694051 None:intergenic 35.0%
!!! TTGACCTCTTTTTCAACCAA+AGG - chr4.4:79694586-79694605 None:intergenic 35.0%
AATTGCAATAACCTCGAGAC+CGG - chr4.4:79694061-79694080 None:intergenic 40.0%
ACATATTGCCAGCCATTTAC+AGG + chr4.4:79694966-79694985 MS.gene50431:CDS 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4.4:79694101-79694120 None:intergenic 40.0%
ACCTAAGTCTATAGTTGCCT+AGG + chr4.4:79695037-79695056 MS.gene50431:intron 40.0%
ACCTAGGCAACTATAGACTT+AGG - chr4.4:79695041-79695060 None:intergenic 40.0%
AGCTCAACAGAAGAGACAAA+AGG + chr4.4:79695359-79695378 MS.gene50431:CDS 40.0%
ATTCTCGTGACCAAGACAAA+CGG - chr4.4:79694756-79694775 None:intergenic 40.0%
CAACAGAAGAGACAAAAGGT+AGG + chr4.4:79695363-79695382 MS.gene50431:CDS 40.0%
CACTTTCCTCAACTCTATTG+AGG + chr4.4:79694092-79694111 MS.gene50431:CDS 40.0%
CAGAATTCAGGAGAAAACTC+TGG - chr4.4:79695463-79695482 None:intergenic 40.0%
CGAATATTGGACTAAGCCTT+TGG + chr4.4:79694567-79694586 MS.gene50431:CDS 40.0%
TTCTGATGCCTTCAACATGT+TGG + chr4.4:79694265-79694284 MS.gene50431:CDS 40.0%
! GACAGGAATGTTGAGTTGTA+GGG - chr4.4:79694372-79694391 None:intergenic 40.0%
! TGACAGGAATGTTGAGTTGT+AGG - chr4.4:79694373-79694392 None:intergenic 40.0%
!! TGATGGGTTATCAGTGAAAC+GGG - chr4.4:79694031-79694050 None:intergenic 40.0%
AATGTGGGGCTTGCGAATAT+TGG + chr4.4:79694554-79694573 MS.gene50431:CDS 45.0%
AGAAGCTACTCACTCAATGC+CGG + chr4.4:79695430-79695449 MS.gene50431:CDS 45.0%
ATTGCGATGCACAACGACAT+TGG + chr4.4:79695501-79695520 MS.gene50431:CDS 45.0%
CAACGACATTGGGTGGAAAT+TGG + chr4.4:79695512-79695531 MS.gene50431:CDS 45.0%
CTACGTGCAGTCAATCAACT+AGG + chr4.4:79694790-79694809 MS.gene50431:CDS 45.0%
GACATTGTGTCGCAGAATTC+AGG - chr4.4:79695475-79695494 None:intergenic 45.0%
TGAAAATGTTGGAAGCTCGG+TGG + chr4.4:79694663-79694682 MS.gene50431:CDS 45.0%
TGATGCTCATATGCCAGACA+TGG + chr4.4:79694319-79694338 MS.gene50431:CDS 45.0%
TTCTCGTGACCAAGACAAAC+GGG - chr4.4:79694755-79694774 None:intergenic 45.0%
TTGCGATGCACAACGACATT+GGG + chr4.4:79695502-79695521 MS.gene50431:CDS 45.0%
AGAAAACTCTGGTCCTGCTC+CGG - chr4.4:79695452-79695471 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4.4:79694743-79694762 MS.gene50431:CDS 50.0%
GATAACCCATCATTCCCAGC+CGG + chr4.4:79694039-79694058 MS.gene50431:CDS 50.0%
CAATAACCTCGAGACCGGCT+GGG - chr4.4:79694056-79694075 None:intergenic 55.0%
CGATGCACAACGACATTGGG+TGG + chr4.4:79695505-79695524 MS.gene50431:CDS 55.0%
TACTCACTCAATGCCGGAGC+AGG + chr4.4:79695436-79695455 MS.gene50431:CDS 55.0%
ATCATTCCCAGCCGGTCTCG+AGG + chr4.4:79694047-79694066 MS.gene50431:CDS 60.0%
CGAGACCGGCTGGGAATGAT+GGG - chr4.4:79694047-79694066 None:intergenic 60.0%
GCAATAACCTCGAGACCGGC+TGG - chr4.4:79694057-79694076 None:intergenic 60.0%
TCGAGACCGGCTGGGAATGA+TGG - chr4.4:79694048-79694067 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 79694024 79695622 79694024 ID=MS.gene50431
chr4.4 mRNA 79694024 79695622 79694024 ID=MS.gene50431.t1;Parent=MS.gene50431
chr4.4 exon 79694024 79694141 79694024 ID=MS.gene50431.t1.exon1;Parent=MS.gene50431.t1
chr4.4 CDS 79694024 79694141 79694024 ID=cds.MS.gene50431.t1;Parent=MS.gene50431.t1
chr4.4 exon 79694246 79694395 79694246 ID=MS.gene50431.t1.exon2;Parent=MS.gene50431.t1
chr4.4 CDS 79694246 79694395 79694246 ID=cds.MS.gene50431.t1;Parent=MS.gene50431.t1
chr4.4 exon 79694509 79694820 79694509 ID=MS.gene50431.t1.exon3;Parent=MS.gene50431.t1
chr4.4 CDS 79694509 79694820 79694509 ID=cds.MS.gene50431.t1;Parent=MS.gene50431.t1
chr4.4 exon 79694911 79694987 79694911 ID=MS.gene50431.t1.exon4;Parent=MS.gene50431.t1
chr4.4 CDS 79694911 79694987 79694911 ID=cds.MS.gene50431.t1;Parent=MS.gene50431.t1
chr4.4 exon 79695338 79695622 79695338 ID=MS.gene50431.t1.exon5;Parent=MS.gene50431.t1
chr4.4 CDS 79695338 79695622 79695338 ID=cds.MS.gene50431.t1;Parent=MS.gene50431.t1
Gene Sequence

>MS.gene50431

ATGGCCCGTTTCACTGATAACCCATCATTCCCAGCCGGTCTCGAGGTTATTGCAATTGATCATGACATCACTTTCCTCAACTCTATTGAGGAGATGTGTAATCGATATCATTATCAAGGTTAGTTACTGAATCATACCTTATTCAAGTTTTCATCTTTTTTGGTAAAAATTCTTACTTTTATATGTATTCATGTTTGTTTATTCTTATATTGTTTATTTCAGTTACCAAATGTAGTATGGCTTCTGATGCCTTCAACATGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAGACATGGATGTTTATGACTTTGTCCAAAATGTTACCCTACAACTCAACATTCCTGTCATTAGTAAGAATTTATTCAACTTTTTCATTAAAATTCATTAGTTACACATTTCACATAATTTATTCATATATATGTACTTAAGAAGTGTGAATTGACATTATATTTCTTTTTTGTAGTGATGGCTGTTGATTCCACAAAAAGTTCTATCATGAAGTCTATTGAATGTGGGGCTTGCGAATATTGGACTAAGCCTTTGGTTGAAAAAGAGGTCAAGATCATGTGGCAACATGTTGCAAGAATTAGAAACGGTTTGATTGAAAATAAAGAATATGAAAATGTTGGAAGCTCGGTGGTTCAAGAAAATACAAAACGGGGTAGAGAAGATGCTAATGCCTCAAAAGAAACACTTGCAAAGAAGGCCCGTTTGTCTTGGTCACGAGAATTGCATCAGCAGTTTCTACGTGCAGTCAATCAACTAGGCCTTGATAGTATGATTCTTTCCTAATTACTTTTTTACTATGCTTTGTTGCATTCTTCAACAGTTGAACTTATGTGCATGTTTTTCTCTTAATTTTCAGGAGCAACGCCAAAAAAGATTCTGAAAATCATGGATGTTCCCGATATGAAAAAAGAACATATTGCCAGCCATTTACAGGTTCATTTTTGTATTAATTTTTGTTGATTCTGTAAATTTGATATATTTTACCTAAGTCTATAGTTGCCTAGGTTTGTCAGTTATCTTTTACCTAAGTCTGTGGTTGTAAATCATTTATGTTGTTTATACCTTAGTCTTGCATGTTATTCCAACTATTGAAGTTGTAAAGTAGTGAATTATGCAGTCATGGCAGATCTTGAAGTCTGAGTTTTAGAATAGAATATAAGTATTTCTCCATTAGCTTCTATACATCCTCTTGTTGATAATATACTACTTGTCTTTTGTGTGATTTTAAACTGAAATTTATGGAGTATTATTATTAACATTGTAACTGCAATGTTGTTTTGCAGAAATATAGAAATCGGTTAAAGAGCTCAACAGAAGAGACAAAAGGTAGGATGAAAAAATCAAAGACGAGTCCTCTTGAAACAAAATTTGACTTAGAAGCTACTCACTCAATGCCGGAGCAGGACCAGAGTTTTCTCCTGAATTCTGCGACACAATGTCGCGACAATTGCGATGCACAACGACATTGGGTGGAAATTGGTGAATCCAACATTATGAGCAACACTTTTTCAGACTTGCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA
Protein sequence

>MS.gene50431.t1

MARFTDNPSFPAGLEVIAIDHDITFLNSIEEMCNRYHYQVTKCSMASDAFNMLERKDCFDVMLIDAHMPDMDVYDFVQNVTLQLNIPVIMMAVDSTKSSIMKSIECGACEYWTKPLVEKEVKIMWQHVARIRNGLIENKEYENVGSSVVQENTKRGREDANASKETLAKKARLSWSRELHQQFLRAVNQLGLDRATPKKILKIMDVPDMKKEHIASHLQKYRNRLKSSTEETKGRMKKSKTSPLETKFDLEATHSMPEQDQSFLLNSATQCRDNCDAQRHWVEIGESNIMSNTFSDLPNLFPYVDDDLSSLVW