AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50549

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50549.t1 MTR_1g071360 88.571 350 32 4 1 349 1 343 0.0 610
MS.gene50549.t1 MTR_1g071360 87.719 342 34 4 4 344 1 335 0.0 589
MS.gene50549.t1 MTR_7g095680 55.714 350 114 9 1 323 1 336 2.14e-108 321
MS.gene50549.t1 MTR_1g102860 46.526 331 132 9 6 319 9 311 1.46e-80 249
MS.gene50549.t1 MTR_3g104550 45.000 300 150 6 20 308 33 328 1.57e-75 238
MS.gene50549.t1 MTR_4g077970 50.000 220 96 2 25 232 4 221 7.16e-67 218
MS.gene50549.t1 MTR_5g029680 50.459 218 90 4 31 233 30 244 6.28e-66 216
MS.gene50549.t1 MTR_1g061170 49.770 217 102 3 20 232 36 249 3.01e-62 206
MS.gene50549.t1 MTR_2g100680 43.564 202 107 3 31 229 29 226 4.84e-52 179
MS.gene50549.t1 MTR_8g087540 38.911 257 132 7 33 268 12 264 1.62e-47 166
MS.gene50549.t1 MTR_6g043060 44.000 200 104 4 33 229 22 216 1.68e-47 164
MS.gene50549.t1 MTR_8g083100 44.828 203 96 5 34 229 23 216 2.07e-47 167
MS.gene50549.t1 MTR_8g105780 44.878 205 99 4 33 223 22 226 2.15e-47 164
MS.gene50549.t1 MTR_6g086805 40.625 224 124 4 33 247 11 234 1.28e-45 159
MS.gene50549.t1 MTR_2g100670 42.932 191 101 3 23 210 27 212 4.17e-45 157
MS.gene50549.t1 MTR_2g100670 42.932 191 101 3 23 210 135 320 5.16e-44 157
MS.gene50549.t1 MTR_5g010680 65.263 95 33 0 31 125 22 116 2.36e-42 150
MS.gene50549.t1 MTR_0020s0020 63.158 95 35 0 31 125 31 125 4.83e-41 147
MS.gene50549.t1 MTR_1g100777 63.158 95 35 0 31 125 16 110 3.31e-40 144
MS.gene50549.t1 MTR_5g017470 42.286 175 62 3 31 166 7 181 5.63e-40 142
MS.gene50549.t1 MTR_5g089170 43.275 171 71 3 31 176 23 192 1.39e-39 140
MS.gene50549.t1 MTR_2g017890 39.086 197 99 4 33 217 15 202 2.28e-39 143
MS.gene50549.t1 MTR_7g091370 42.246 187 79 3 24 191 9 185 6.51e-39 138
MS.gene50549.t1 MTR_3g101870 65.657 99 33 1 31 129 22 119 6.85e-39 142
MS.gene50549.t1 MTR_4g088900 37.640 178 97 3 31 201 31 201 6.22e-34 124
MS.gene50549.t1 MTR_1g061170 41.497 147 79 3 90 232 11 154 3.39e-28 113
MS.gene50549.t1 MTR_5g047190 42.857 91 45 2 41 124 1 91 1.59e-16 74.7
MS.gene50549.t1 MTR_4g045757 53.030 66 30 1 33 97 14 79 4.99e-15 69.7
MS.gene50549.t1 MTR_6g044340 32.474 194 105 9 93 275 22 200 4.19e-14 72.0
MS.gene50549.t1 MTR_2g043940 26.108 203 120 5 64 248 3 193 2.98e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50549.t1 AT3G22830 48.358 335 133 8 13 308 41 374 3.98e-93 284
MS.gene50549.t1 AT3G51910 52.962 287 90 9 24 308 20 263 1.74e-86 262
MS.gene50549.t1 AT3G63350 44.136 324 125 7 18 339 13 282 1.42e-79 245
MS.gene50549.t1 AT2G26150 50.230 217 102 2 20 230 31 247 3.59e-72 228
MS.gene50549.t1 AT2G26150 50.230 217 102 2 20 230 31 247 3.59e-72 228
MS.gene50549.t1 AT1G32330 50.472 212 78 3 18 208 22 227 1.03e-64 213
MS.gene50549.t1 AT1G32330 50.472 212 78 3 18 208 22 227 1.23e-64 213
MS.gene50549.t1 AT5G43840 41.522 289 142 7 20 297 6 278 2.17e-64 206
MS.gene50549.t1 AT4G17750 43.561 264 109 5 15 247 31 285 8.51e-63 208
MS.gene50549.t1 AT5G16820 49.123 228 95 4 14 229 16 234 1.14e-61 204
MS.gene50549.t1 AT5G16820 49.123 228 95 4 14 229 16 234 1.14e-61 204
MS.gene50549.t1 AT3G02990 48.325 209 93 4 33 229 23 228 5.99e-59 197
MS.gene50549.t1 AT5G03720 48.500 200 98 3 33 228 55 253 9.50e-57 190
MS.gene50549.t1 AT5G03720 48.500 200 98 3 33 228 55 253 9.50e-57 190
MS.gene50549.t1 AT1G67970 46.698 212 103 4 33 237 19 227 8.95e-55 184
MS.gene50549.t1 AT5G54070 41.089 202 112 3 33 231 71 268 3.06e-50 171
MS.gene50549.t1 AT4G13980 43.284 201 107 3 34 231 24 220 1.06e-49 173
MS.gene50549.t1 AT3G24520 40.909 198 100 2 33 230 17 197 8.78e-46 159
MS.gene50549.t1 AT4G18880 45.455 187 95 2 23 202 5 191 1.18e-40 147
MS.gene50549.t1 AT1G46264 40.265 226 79 5 24 195 25 248 1.55e-40 145
MS.gene50549.t1 AT4G36990 64.894 94 33 0 31 124 12 105 4.33e-40 143
MS.gene50549.t1 AT5G62020 62.105 95 36 0 31 125 21 115 6.64e-39 140
MS.gene50549.t1 AT4G11660 62.626 99 36 1 31 129 57 154 1.06e-38 142
MS.gene50549.t1 AT5G45710 37.143 210 126 2 33 236 13 222 4.24e-38 139
MS.gene50549.t1 AT5G45710 37.143 210 126 2 33 236 13 222 4.24e-38 139
MS.gene50549.t1 AT2G41690 52.381 126 46 2 31 143 38 162 3.48e-36 131
MS.gene50549.t1 AT2G26150 44.056 143 74 2 93 229 49 191 1.02e-32 124
MS.gene50549.t1 AT2G26150 44.056 143 74 2 93 229 49 191 1.02e-32 124
MS.gene50549.t1 AT4G18870 52.809 89 37 2 34 120 14 99 1.93e-23 98.6
MS.gene50549.t1 AT4G18870 46.591 88 47 0 33 120 146 233 2.22e-21 93.2
MS.gene50549.t1 AT1G77570 35.227 88 54 2 34 118 27 114 1.02e-11 62.8

Find 69 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGTCCTTGTTGGGAATT+AGG 0.117532 1.2:+54665050 None:intergenic
GAATTACGTGATTATGAATT+TGG 0.128353 1.2:-54664693 MS.gene50549:CDS
ATGCAGAAATTGATCGTTTA+AGG 0.140595 1.2:-54665013 MS.gene50549:CDS
ATTATTGTGTTTGAAGTATC+TGG 0.159629 1.2:+54665379 None:intergenic
TTCTGTTCCATGAAATTATA+TGG 0.235121 1.2:+54665456 None:intergenic
CAACAAGGACAAGGTTGTTT+TGG 0.253139 1.2:-54665041 MS.gene50549:CDS
AACAAGTCCTTCATTTGGTT+GGG 0.266336 1.2:+54665541 None:intergenic
AATTACGTGATTATGAATTT+GGG 0.282722 1.2:-54664692 MS.gene50549:CDS
AACCACTGAGTCAAGAATTT+AGG 0.293001 1.2:-54664440 MS.gene50549:CDS
TTTGCAAATTCCCATCTATC+TGG 0.293795 1.2:+54665146 None:intergenic
CCTTCATTTGGTTGGGGTGG+TGG 0.306285 1.2:+54665548 None:intergenic
ACAACAGAAAGAGAAGAAAA+AGG 0.330208 1.2:-54664817 MS.gene50549:CDS
TCTTCTCTTTCTGTTGTAGT+TGG 0.332869 1.2:+54664823 None:intergenic
CAGATGGAGCAAAGGTTAGA+AGG 0.354438 1.2:-54664915 MS.gene50549:CDS
TGCTTGATGATGAAGGGTTC+TGG 0.373815 1.2:-54664551 MS.gene50549:CDS
TCATTTGGTTGGGGTGGTGG+TGG 0.381814 1.2:+54665551 None:intergenic
CCTTGTTGGGAATTAGGAAT+AGG 0.386492 1.2:+54665056 None:intergenic
ATTTCAACAAGTCCTTCATT+TGG 0.388996 1.2:+54665536 None:intergenic
GTACCAACCATATAATTTCA+TGG 0.396015 1.2:-54665463 MS.gene50549:CDS
CAAAACAACCTTGTCCTTGT+TGG 0.397714 1.2:+54665042 None:intergenic
AAAACAACCTTGTCCTTGTT+GGG 0.399773 1.2:+54665043 None:intergenic
TCTTGTGATTCTAGTGCCTC+AGG 0.405629 1.2:+54664588 None:intergenic
TGAATTTGGGGTGTCGGAGT+TGG 0.413715 1.2:-54664679 MS.gene50549:CDS
TGTATCCCAAACCACAAAAC+TGG 0.420129 1.2:+54665427 None:intergenic
CAACAAGTCCTTCATTTGGT+TGG 0.420416 1.2:+54665540 None:intergenic
CTCGGTGAAAGAAGAATACT+TGG 0.431235 1.2:-54665585 MS.gene50549:CDS
TCATTGTTATCTTCAGAAGT+TGG 0.432296 1.2:+54664483 None:intergenic
ATAGATGTCTTTGCCCTCTC+AGG 0.446863 1.2:+54665114 None:intergenic
TGGGGTGGTGGTGGAATCGT+TGG 0.458015 1.2:+54665560 None:intergenic
GTATCTGGGAAGGAGATGAT+TGG 0.458621 1.2:+54665394 None:intergenic
ACAAGTCCTTCATTTGGTTG+GGG 0.464149 1.2:+54665542 None:intergenic
GGGGAAAATAATGGATAAAG+AGG 0.470921 1.2:-54664622 MS.gene50549:CDS
AGTCCTTCATTTGGTTGGGG+TGG 0.486652 1.2:+54665545 None:intergenic
GTTCCATGAAATTATATGGT+TGG 0.492988 1.2:+54665460 None:intergenic
ATGTTTAGTATGAAATTTGA+GGG 0.495224 1.2:-54664519 MS.gene50549:CDS
TGATTATGAATTTGGGGTGT+CGG 0.498346 1.2:-54664685 MS.gene50549:CDS
GCTTGATGATGAAGGGTTCT+GGG 0.499647 1.2:-54664550 MS.gene50549:CDS
TTATTGTGTTTGAAGTATCT+GGG 0.502771 1.2:+54665380 None:intergenic
CAAGGAACTAGACTAGGTAT+TGG 0.504481 1.2:-54664747 MS.gene50549:CDS
AATGTTTAGTATGAAATTTG+AGG 0.507525 1.2:-54664520 MS.gene50549:CDS
TACACTTTATATTCTACAGA+TGG 0.519014 1.2:-54664931 MS.gene50549:CDS
GAGGAACATAAGAAGAAAGA+AGG 0.536879 1.2:-54665090 MS.gene50549:CDS
ACTAGAATCACAAGAGAAGA+TGG 0.536908 1.2:-54664580 MS.gene50549:CDS
CCACCACCCCAACCAAATGA+AGG 0.544676 1.2:-54665548 MS.gene50549:CDS
AATGCTAGCTATGGAAATGC+AGG 0.546390 1.2:-54664655 MS.gene50549:CDS
GGAGTTGGAAATGCTAGCTA+TGG 0.547153 1.2:-54664664 MS.gene50549:CDS
TGTGTTTGAAGTATCTGGGA+AGG 0.547658 1.2:+54665384 None:intergenic
CAAATGAAAGATTCCTGAGA+GGG 0.550958 1.2:-54665127 MS.gene50549:CDS
ATTACGTGATTATGAATTTG+GGG 0.552478 1.2:-54664691 MS.gene50549:CDS
CCTAATTCCCAACAAGGACA+AGG 0.555251 1.2:-54665050 MS.gene50549:CDS
GAAAGAGTGCTTGATGATGA+AGG 0.560834 1.2:-54664558 MS.gene50549:CDS
TGTAGTTGGTGAATGAAGAC+AGG 0.561937 1.2:+54664837 None:intergenic
AGATGGAGCAAAGGTTAGAA+GGG 0.566766 1.2:-54664914 MS.gene50549:CDS
ATATTCTACAGATGGAGCAA+AGG 0.570553 1.2:-54664923 MS.gene50549:CDS
ATCCTAAATTCTTGACTCAG+TGG 0.576729 1.2:+54664438 None:intergenic
AAGAGAAAGCTTGTTGAACA+AGG 0.580615 1.2:-54664765 MS.gene50549:CDS
GAATTAGGAATAGGCTGAGA+AGG 0.581146 1.2:+54665065 None:intergenic
TCATAATCACGTAATTCTAA+CGG 0.586096 1.2:+54664699 None:intergenic
GGGAAGGAGATGATTGGAGA+AGG 0.595385 1.2:+54665400 None:intergenic
GAGAATGAATTATATGTACT+CGG 0.595766 1.2:-54665603 None:intergenic
GAGGGCAAAGACATCTATTG+AGG 0.598712 1.2:-54665109 MS.gene50549:CDS
ATAATTTCATGGAACAGAAG+TGG 0.614186 1.2:-54665452 MS.gene50549:CDS
GCAAATGAAAGATTCCTGAG+AGG 0.614961 1.2:-54665128 MS.gene50549:CDS
CCTATTCCTAATTCCCAACA+AGG 0.619154 1.2:-54665056 MS.gene50549:CDS
AGAGAAACACGTGTTGATGA+CGG 0.622649 1.2:-54664988 MS.gene50549:CDS
GTTGAACAAGGAACTAGACT+AGG 0.638387 1.2:-54664753 MS.gene50549:CDS
AGAGGAAGTACATGAACCTG+AGG 0.670115 1.2:-54664604 MS.gene50549:CDS
AAAGAGTGCTTGATGATGAA+GGG 0.670867 1.2:-54664557 MS.gene50549:CDS
GTAGTTGGTGAATGAAGACA+GGG 0.704135 1.2:+54664838 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTTAGTATGAAATTTG+AGG - chr1.2:54665492-54665511 MS.gene50549:CDS 20.0%
!! AATTACGTGATTATGAATTT+GGG - chr1.2:54665320-54665339 MS.gene50549:intron 20.0%
!! ATGTTTAGTATGAAATTTGA+GGG - chr1.2:54665493-54665512 MS.gene50549:CDS 20.0%
!! TTACAGTTCAAATTTATTCT+CGG - chr1.2:54664775-54664794 MS.gene50549:CDS 20.0%
! AAAAATTGATCCAGATAGAT+GGG - chr1.2:54664856-54664875 MS.gene50549:CDS 25.0%
! ATTACGTGATTATGAATTTG+GGG - chr1.2:54665321-54665340 MS.gene50549:intron 25.0%
! GAATTACGTGATTATGAATT+TGG - chr1.2:54665319-54665338 MS.gene50549:intron 25.0%
! TACACTTTATATTCTACAGA+TGG - chr1.2:54665081-54665100 MS.gene50549:CDS 25.0%
! TCATAATCACGTAATTCTAA+CGG + chr1.2:54665316-54665335 None:intergenic 25.0%
! TTCTGTTCCATGAAATTATA+TGG + chr1.2:54664559-54664578 None:intergenic 25.0%
!! ATTATTGTGTTTGAAGTATC+TGG + chr1.2:54664636-54664655 None:intergenic 25.0%
!! GTGTTATACAATATTTTGCA+GGG - chr1.2:54664827-54664846 MS.gene50549:CDS 25.0%
!! TGTGTTATACAATATTTTGC+AGG - chr1.2:54664826-54664845 MS.gene50549:CDS 25.0%
!! TTATTGTGTTTGAAGTATCT+GGG + chr1.2:54664635-54664654 None:intergenic 25.0%
ACAACAGAAAGAGAAGAAAA+AGG - chr1.2:54665195-54665214 MS.gene50549:intron 30.0%
ATAATTTCATGGAACAGAAG+TGG - chr1.2:54664560-54664579 MS.gene50549:CDS 30.0%
ATGCAGAAATTGATCGTTTA+AGG - chr1.2:54664999-54665018 MS.gene50549:CDS 30.0%
ATTTCAACAAGTCCTTCATT+TGG + chr1.2:54664479-54664498 None:intergenic 30.0%
GAAAAATTGATCCAGATAGA+TGG - chr1.2:54664855-54664874 MS.gene50549:CDS 30.0%
GTACCAACCATATAATTTCA+TGG - chr1.2:54664549-54664568 MS.gene50549:CDS 30.0%
GTTCCATGAAATTATATGGT+TGG + chr1.2:54664555-54664574 None:intergenic 30.0%
TCATTGTTATCTTCAGAAGT+TGG + chr1.2:54665532-54665551 None:intergenic 30.0%
!! CTACATCAAATGTTTTTGTG+AGG + chr1.2:54664517-54664536 None:intergenic 30.0%
!! TCAAATGTTTTTGTGAGGAA+TGG + chr1.2:54664512-54664531 None:intergenic 30.0%
!!! AAGCTTTCTCTTTTTTGTCA+TGG + chr1.2:54665239-54665258 None:intergenic 30.0%
!!! ATGTTTTCTCGTACTGATTT+TGG - chr1.2:54664800-54664819 MS.gene50549:CDS 30.0%
AAAACAACCTTGTCCTTGTT+GGG + chr1.2:54664972-54664991 None:intergenic 35.0%
AACAAGTCCTTCATTTGGTT+GGG + chr1.2:54664474-54664493 None:intergenic 35.0%
AACCACTGAGTCAAGAATTT+AGG - chr1.2:54665572-54665591 MS.gene50549:CDS 35.0%
ACTAGAATCACAAGAGAAGA+TGG - chr1.2:54665432-54665451 MS.gene50549:CDS 35.0%
ATATTCTACAGATGGAGCAA+AGG - chr1.2:54665089-54665108 MS.gene50549:CDS 35.0%
ATCCTAAATTCTTGACTCAG+TGG + chr1.2:54665577-54665596 None:intergenic 35.0%
CAAATGAAAGATTCCTGAGA+GGG - chr1.2:54664885-54664904 MS.gene50549:CDS 35.0%
GAGGAACATAAGAAGAAAGA+AGG - chr1.2:54664922-54664941 MS.gene50549:CDS 35.0%
TCTTCTCTTTCTGTTGTAGT+TGG + chr1.2:54665192-54665211 None:intergenic 35.0%
TGATCCTAATGTCTCTATCT+AGG - chr1.2:54664718-54664737 MS.gene50549:CDS 35.0%
TTTGCAAATTCCCATCTATC+TGG + chr1.2:54664869-54664888 None:intergenic 35.0%
! TGATTATGAATTTGGGGTGT+CGG - chr1.2:54665327-54665346 MS.gene50549:intron 35.0%
!! AAAGAGTGCTTGATGATGAA+GGG - chr1.2:54665455-54665474 MS.gene50549:CDS 35.0%
!! AAGAGAAAGCTTGTTGAACA+AGG - chr1.2:54665247-54665266 MS.gene50549:intron 35.0%
!! GGGGAAAATAATGGATAAAG+AGG - chr1.2:54665390-54665409 MS.gene50549:CDS 35.0%
!! TTTTGGAAGGGGGAAAATAA+TGG - chr1.2:54665381-54665400 MS.gene50549:CDS 35.0%
AATGCTAGCTATGGAAATGC+AGG - chr1.2:54665357-54665376 MS.gene50549:CDS 40.0%
ACAAGTCCTTCATTTGGTTG+GGG + chr1.2:54664473-54664492 None:intergenic 40.0%
AGATGGAGCAAAGGTTAGAA+GGG - chr1.2:54665098-54665117 MS.gene50549:CDS 40.0%
CAAAACAACCTTGTCCTTGT+TGG + chr1.2:54664973-54664992 None:intergenic 40.0%
CAACAAGTCCTTCATTTGGT+TGG + chr1.2:54664475-54664494 None:intergenic 40.0%
CAAGGAACTAGACTAGGTAT+TGG - chr1.2:54665265-54665284 MS.gene50549:intron 40.0%
CCTATTCCTAATTCCCAACA+AGG - chr1.2:54664956-54664975 MS.gene50549:CDS 40.0%
CCTTGTTGGGAATTAGGAAT+AGG + chr1.2:54664959-54664978 None:intergenic 40.0%
CTCGGTGAAAGAAGAATACT+TGG - chr1.2:54664427-54664446 MS.gene50549:CDS 40.0%
CTGACCTAGATAGAGACATT+AGG + chr1.2:54664725-54664744 None:intergenic 40.0%
GAATTAGGAATAGGCTGAGA+AGG + chr1.2:54664950-54664969 None:intergenic 40.0%
GCAAATGAAAGATTCCTGAG+AGG - chr1.2:54664884-54664903 MS.gene50549:CDS 40.0%
GTTGAACAAGGAACTAGACT+AGG - chr1.2:54665259-54665278 MS.gene50549:intron 40.0%
TGTATCCCAAACCACAAAAC+TGG + chr1.2:54664588-54664607 None:intergenic 40.0%
! AGAGAAACACGTGTTGATGA+CGG - chr1.2:54665024-54665043 MS.gene50549:CDS 40.0%
! CAACAAGGACAAGGTTGTTT+TGG - chr1.2:54664971-54664990 MS.gene50549:CDS 40.0%
! GCTATGGAAATGCAGGTTTT+TGG - chr1.2:54665364-54665383 MS.gene50549:CDS 40.0%
! GTTTTTGTGAGGAATGGTTG+TGG + chr1.2:54664506-54664525 None:intergenic 40.0%
! TGTGTTTGAAGTATCTGGGA+AGG + chr1.2:54664631-54664650 None:intergenic 40.0%
!! AAATGCAGGTTTTTGGAAGG+GGG - chr1.2:54665371-54665390 MS.gene50549:CDS 40.0%
!! GAAAGAGTGCTTGATGATGA+AGG - chr1.2:54665454-54665473 MS.gene50549:CDS 40.0%
!! GAAATGCAGGTTTTTGGAAG+GGG - chr1.2:54665370-54665389 MS.gene50549:CDS 40.0%
!! GGAAATGCAGGTTTTTGGAA+GGG - chr1.2:54665369-54665388 MS.gene50549:CDS 40.0%
!! GTAGTTGGTGAATGAAGACA+GGG + chr1.2:54665177-54665196 None:intergenic 40.0%
!! TGGAAATGCAGGTTTTTGGA+AGG - chr1.2:54665368-54665387 MS.gene50549:CDS 40.0%
!! TGTAGTTGGTGAATGAAGAC+AGG + chr1.2:54665178-54665197 None:intergenic 40.0%
!!! TCTCTTTTTTGTCATGGTCG+CGG + chr1.2:54665233-54665252 None:intergenic 40.0%
ATAGATGTCTTTGCCCTCTC+AGG + chr1.2:54664901-54664920 None:intergenic 45.0%
CAGATGGAGCAAAGGTTAGA+AGG - chr1.2:54665097-54665116 MS.gene50549:CDS 45.0%
CCTAATTCCCAACAAGGACA+AGG - chr1.2:54664962-54664981 MS.gene50549:CDS 45.0%
CCTTGTCCTTGTTGGGAATT+AGG + chr1.2:54664965-54664984 None:intergenic 45.0%
GAGGGCAAAGACATCTATTG+AGG - chr1.2:54664903-54664922 MS.gene50549:CDS 45.0%
GGAGTTGGAAATGCTAGCTA+TGG - chr1.2:54665348-54665367 MS.gene50549:CDS 45.0%
GTATCTGGGAAGGAGATGAT+TGG + chr1.2:54664621-54664640 None:intergenic 45.0%
TCTTGTGATTCTAGTGCCTC+AGG + chr1.2:54665427-54665446 None:intergenic 45.0%
! AGAGGAAGTACATGAACCTG+AGG - chr1.2:54665408-54665427 MS.gene50549:CDS 45.0%
!! GCTTGATGATGAAGGGTTCT+GGG - chr1.2:54665462-54665481 MS.gene50549:CDS 45.0%
!! TGCTTGATGATGAAGGGTTC+TGG - chr1.2:54665461-54665480 MS.gene50549:CDS 45.0%
!! TGGCACCAGTTTTGTGGTTT+GGG - chr1.2:54664580-54664599 MS.gene50549:CDS 45.0%
AGTCCTTCATTTGGTTGGGG+TGG + chr1.2:54664470-54664489 None:intergenic 50.0%
GGGAAGGAGATGATTGGAGA+AGG + chr1.2:54664615-54664634 None:intergenic 50.0%
! CAGAAGTGGCACCAGTTTTG+TGG - chr1.2:54664574-54664593 MS.gene50549:CDS 50.0%
!! GTGGCACCAGTTTTGTGGTT+TGG - chr1.2:54664579-54664598 MS.gene50549:CDS 50.0%
!! TGAATTTGGGGTGTCGGAGT+TGG - chr1.2:54665333-54665352 MS.gene50549:intron 50.0%
CCACCACCCCAACCAAATGA+AGG - chr1.2:54664464-54664483 MS.gene50549:CDS 55.0%
CCTTCATTTGGTTGGGGTGG+TGG + chr1.2:54664467-54664486 None:intergenic 55.0%
! TCATTTGGTTGGGGTGGTGG+TGG + chr1.2:54664464-54664483 None:intergenic 55.0%
!! TGGGGTGGTGGTGGAATCGT+TGG + chr1.2:54664455-54664474 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 54664413 54665621 54664413 ID=MS.gene50549
chr1.2 mRNA 54664413 54665621 54664413 ID=MS.gene50549.t1;Parent=MS.gene50549
chr1.2 exon 54665346 54665621 54665346 ID=MS.gene50549.t1.exon1;Parent=MS.gene50549.t1
chr1.2 CDS 54665346 54665621 54665346 ID=cds.MS.gene50549.t1;Parent=MS.gene50549.t1
chr1.2 exon 54664413 54665186 54664413 ID=MS.gene50549.t1.exon2;Parent=MS.gene50549.t1
chr1.2 CDS 54664413 54665186 54664413 ID=cds.MS.gene50549.t1;Parent=MS.gene50549.t1
Gene Sequence

>MS.gene50549

ATGAATTATATGTACTCGGTGAAAGAAGAATACTTGGAACCAACGATTCCACCACCACCCCAACCAAATGAAGGACTTGTTGAAATTAGACCACAACCATTCCTCACAAAAACATTTGATGTAGTTGATGATCAAAGTACCAACCATATAATTTCATGGAACAGAAGTGGCACCAGTTTTGTGGTTTGGGATACACATGCCTTCTCCAATCATCTCCTTCCCAGATACTTCAAACACAATAATTTCTCAAGTTTTGTCAGACAATTAAACACTTATGTAAGTACTACGTACTCTCTAGATACACATGATCCTAATGTCTCTATCTAGGTCAGATTTTAAAACATTGCTTATATAGTAAGTAATTACAGTTCAAATTTATTCTCGGCTATGTTTTCTCGTACTGATTTTGGACTTGTGTTATACAATATTTTGCAGGGATTTAGAAAAATTGATCCAGATAGATGGGAATTTGCAAATGAAAGATTCCTGAGAGGGCAAAGACATCTATTGAGGAACATAAGAAGAAAGAAGGTGCCTTCTCAGCCTATTCCTAATTCCCAACAAGGACAAGGTTGTTTTGGACTAGATGCAGAAATTGATCGTTTAAGGCAAGAGAAACACGTGTTGATGACGGAGCTTATGAGTCTTAGAAAGCAGCAATATAATACTACACTTTATATTCTACAGATGGAGCAAAGGTTAGAAGGGATTGAAATCAATCAGAAGAAAATGATGTCTTTCTTAGCTAGAGCTATGAAAAACCCTGTCTTCATTCACCAACTACAACAGAAAGAGAAGAAAAAGGAGCTTGAAGAATCCGCGACCATGACAAAAAAGAGAAAGCTTGTTGAACAAGGAACTAGACTAGGTATTGGACAATCAAGCAGTGTTAAAGTTGAGCCGTTAGAATTACGTGATTATGAATTTGGGGTGTCGGAGTTGGAAATGCTAGCTATGGAAATGCAGGTTTTTGGAAGGGGGAAAATAATGGATAAAGAGGAAGTACATGAACCTGAGGCACTAGAATCACAAGAGAAGATGGAAAGAGTGCTTGATGATGAAGGGTTCTGGGAAGATCTAATGTTTAGTATGAAATTTGAGGGTAGATTTGACATTCCAACTTCTGAAGATAACAATGAGAATGAAGATGATTCAAGTGAACCACTGAGTCAAGAATTTAGGATTAACTCTTTTCATTCAGTTCACTAG
Protein sequence

>MS.gene50549.t1

MNYMYSVKEEYLEPTIPPPPQPNEGLVEIRPQPFLTKTFDVVDDQSTNHIISWNRSGTSFVVWDTHAFSNHLLPRYFKHNNFSSFVRQLNTYGFRKIDPDRWEFANERFLRGQRHLLRNIRRKKVPSQPIPNSQQGQGCFGLDAEIDRLRQEKHVLMTELMSLRKQQYNTTLYILQMEQRLEGIEINQKKMMSFLARAMKNPVFIHQLQQKEKKKELEESATMTKKRKLVEQGTRLGIGQSSSVKVEPLELRDYEFGVSELEMLAMEMQVFGRGKIMDKEEVHEPEALESQEKMERVLDDEGFWEDLMFSMKFEGRFDIPTSEDNNENEDDSSEPLSQEFRINSFHSVH