AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50715

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50715.t1 MTR_4g071590 96.959 296 8 1 1 296 1 295 0.0 596
MS.gene50715.t1 MTR_4g471590 96.959 296 8 1 1 296 1 295 0.0 596
MS.gene50715.t1 MTR_3g117380 53.292 319 107 8 1 296 15 314 3.38e-97 289
MS.gene50715.t1 MTR_8g076340 39.159 309 138 10 1 281 1 287 1.17e-51 171
MS.gene50715.t1 MTR_5g021340 40.282 355 137 11 1 296 51 389 3.44e-50 171
MS.gene50715.t1 MTR_3g109760 38.406 276 120 9 36 281 21 276 4.55e-45 155
MS.gene50715.t1 MTR_8g013950 38.869 283 113 9 39 281 33 295 2.90e-43 151
MS.gene50715.t1 MTR_1g006650 34.921 315 141 12 15 281 5 303 1.39e-42 149
MS.gene50715.t1 MTR_1g094930 75.714 70 17 0 207 276 142 211 5.69e-35 126
MS.gene50715.t1 MTR_1g094930 75.714 70 17 0 207 276 237 306 1.71e-34 128
MS.gene50715.t1 MTR_6g032830 81.538 65 12 0 212 276 192 256 2.67e-34 125
MS.gene50715.t1 MTR_7g085410 80.000 65 13 0 212 276 188 252 2.88e-33 123
MS.gene50715.t1 MTR_1g116010 67.532 77 25 0 212 288 164 240 4.65e-33 122
MS.gene50715.t1 MTR_1g046680 76.471 68 16 0 212 279 172 239 1.05e-32 121
MS.gene50715.t1 MTR_2g094600 62.791 86 30 1 191 276 197 280 3.26e-32 121
MS.gene50715.t1 MTR_4g058910 73.134 67 18 0 212 278 314 380 1.59e-31 121
MS.gene50715.t1 MTR_5g007600 68.571 70 22 0 212 281 259 328 1.22e-29 115
MS.gene50715.t1 MTR_5g007600 59.036 83 28 1 199 275 138 220 2.68e-26 106
MS.gene50715.t1 MTR_7g446110 57.143 63 27 0 211 273 186 248 2.23e-20 88.6
MS.gene50715.t1 MTR_7g445950 30.128 156 105 1 118 273 30 181 2.52e-20 86.7
MS.gene50715.t1 MTR_7g445980 51.316 76 36 1 199 273 20 95 3.03e-19 81.3
MS.gene50715.t1 MTR_7g446070 31.868 182 107 5 105 273 16 193 6.41e-18 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50715.t1 AT4G36240 47.082 257 90 10 40 282 10 234 1.36e-56 182
MS.gene50715.t1 AT5G66320 35.838 346 139 10 8 291 5 329 2.34e-51 172
MS.gene50715.t1 AT5G66320 35.838 346 139 10 8 291 5 329 2.34e-51 172
MS.gene50715.t1 AT4G34680 41.034 290 114 11 6 283 7 251 1.97e-49 165
MS.gene50715.t1 AT4G34680 41.034 290 114 11 6 283 7 251 1.97e-49 165
MS.gene50715.t1 AT5G25830 38.047 297 108 12 40 281 13 288 1.45e-44 154
MS.gene50715.t1 AT3G60530 47.778 180 73 5 112 283 63 229 2.27e-43 149
MS.gene50715.t1 AT4G32890 36.331 278 115 11 39 281 16 266 4.71e-43 150
MS.gene50715.t1 AT2G45050 44.845 194 73 4 112 281 65 248 4.87e-43 148
MS.gene50715.t1 AT3G51080 69.231 91 22 2 212 296 221 311 2.45e-38 137
MS.gene50715.t1 AT3G24050 64.706 85 29 1 197 280 178 262 1.02e-33 124
MS.gene50715.t1 AT3G54810 75.000 68 17 0 212 279 229 296 3.72e-33 124
MS.gene50715.t1 AT3G54810 75.000 68 17 0 212 279 229 296 3.72e-33 124
MS.gene50715.t1 AT1G08010 72.464 69 19 0 212 280 220 288 3.50e-32 121
MS.gene50715.t1 AT1G08010 72.464 69 19 0 212 280 220 288 3.50e-32 121
MS.gene50715.t1 AT1G08010 72.464 69 19 0 212 280 220 288 3.50e-32 121
MS.gene50715.t1 AT1G08000 71.014 69 20 0 212 280 218 286 2.71e-30 116
MS.gene50715.t1 AT1G08000 71.014 69 20 0 212 280 218 286 2.71e-30 116
MS.gene50715.t1 AT3G45170 60.274 73 29 0 214 286 117 189 9.39e-27 104
MS.gene50715.t1 AT2G28340 63.077 65 24 0 214 278 193 257 1.60e-24 100

Find 89 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTGTCTTGGTTGTTCT+AGG 0.195513 4.3:+33899946 None:intergenic
AGCCCCACCCGTTTGGGCTT+CGG 0.226880 4.3:+33899858 None:intergenic
GCCCCACCCGTTTGGGCTTC+GGG 0.232140 4.3:+33899859 None:intergenic
GGCCGATGATGTTGCGGATT+TGG 0.241470 4.3:-33900103 MS.gene50715:CDS
GAACAGCCACCGTAAGGTTT+TGG 0.270098 4.3:-33899662 MS.gene50715:CDS
GAGGTGGATGAACCGGTTTC+CGG 0.310815 4.3:-33899622 MS.gene50715:CDS
GGTTCGGCCCGGGCCTGAAC+TGG 0.312951 4.3:+33900027 None:intergenic
TGCCGGTGGTTTAGCAGTTC+CGG 0.331211 4.3:-33900381 MS.gene50715:intron
GAAACTGAGCCCCACCCGTT+TGG 0.331959 4.3:+33899851 None:intergenic
TCTTTGCTGAAAAGAGTATA+GGG 0.337151 4.3:+33899896 None:intergenic
TCTCTTCACAAATTGTTTGT+TGG 0.346081 4.3:+33900660 None:intergenic
AAACTGAGCCCCACCCGTTT+GGG 0.350163 4.3:+33899852 None:intergenic
CTTCTTCTTCTTCCTCCTCC+TGG 0.352825 4.3:+33900519 None:intergenic
CTCTCTATTGTATCCAGTTC+AGG 0.364580 4.3:-33900040 MS.gene50715:CDS
AACCGGTTTCCGGTCTGAAC+CGG 0.370991 4.3:-33899612 MS.gene50715:CDS
GCATTGGTTGGGCCCGGTCT+AGG 0.372639 4.3:+33900000 None:intergenic
ACAGTTGGGCCTTCTTGTCT+TGG 0.379924 4.3:+33899936 None:intergenic
GTTGGGCCCGGTCTAGGTTC+CGG 0.399228 4.3:+33900006 None:intergenic
AGTTCAGGCCCGGGCCGAAC+CGG 0.407384 4.3:-33900025 MS.gene50715:CDS
GTCTAGGTTCCGGTTCGGCC+CGG 0.414385 4.3:+33900016 None:intergenic
GGCCGAACCGGAACCTAGAC+CGG 0.414860 4.3:-33900013 MS.gene50715:CDS
TTCTTTGCTGAAAAGAGTAT+AGG 0.416988 4.3:+33899895 None:intergenic
AACGCAAACAACGTTGTTGT+CGG 0.417578 4.3:-33900626 MS.gene50715:CDS
TTTGGAAATGAGGCGGAGGA+AGG 0.432787 4.3:-33899644 MS.gene50715:CDS
TATGTAACGCTTGTGGTGTT+CGG 0.433641 4.3:-33899756 MS.gene50715:CDS
GAATTTCACCGGAAAACGTC+GGG 0.435549 4.3:+33899689 None:intergenic
GAATTTCAACATGGTTCTGT+TGG 0.444517 4.3:-33900560 MS.gene50715:CDS
TATTGTATCCAGTTCAGGCC+CGG 0.446736 4.3:-33900035 MS.gene50715:CDS
GGAATGGGTGTCTCACTTTG+TGG 0.449579 4.3:-33900082 MS.gene50715:CDS
AATGAGGCGGAGGAAGGAGG+TGG 0.451531 4.3:-33899638 MS.gene50715:CDS
ATCCGGTTCAGACCGGAAAC+CGG 0.452815 4.3:+33899610 None:intergenic
GAGGAAGGAGGTGGATGAAC+CGG 0.452883 4.3:-33899629 MS.gene50715:CDS
TCTAGGTTCCGGTTCGGCCC+GGG 0.468440 4.3:+33900017 None:intergenic
ATAGATTCTGTCCTCAAGCT+TGG 0.468461 4.3:+33900686 None:intergenic
GCCTCATTTCCAAAACCTTA+CGG 0.469337 4.3:+33899653 None:intergenic
TTCTGCGGATTATAGAGAGC+TGG 0.472534 4.3:+33899971 None:intergenic
CGGTCTGAACCGGATTCAAA+TGG 0.475660 4.3:-33899602 MS.gene50715:CDS
ATGATGTTGCGGATTTGGAA+TGG 0.479723 4.3:-33900098 MS.gene50715:CDS
AACTCGTGACCATTTGAATC+CGG 0.481371 4.3:+33899593 None:intergenic
CAGTGGAGAGCTGGTCCGCT+TGG 0.483307 4.3:-33899790 MS.gene50715:CDS
GGAGTCAGACTCGCCGTCTC+CGG 0.485968 4.3:+33900409 None:intergenic
TGTGGTGTTCGGTTTAAGTC+CGG 0.492368 4.3:-33899745 MS.gene50715:CDS
GGATACAATAGAGAGAGTTC+TGG 0.493646 4.3:+33900048 None:intergenic
GCCCGAAGCCCAAACGGGTG+GGG 0.493923 4.3:-33899860 MS.gene50715:CDS
AAAATCCACACGTTACAGTT+GGG 0.498357 4.3:+33899922 None:intergenic
TGATGTTGCGGATTTGGAAT+GGG 0.501793 4.3:-33900097 MS.gene50715:CDS
GGAAATGAGGCGGAGGAAGG+AGG 0.505698 4.3:-33899641 MS.gene50715:CDS
GCCCGGTCTAGGTTCCGGTT+CGG 0.510484 4.3:+33900011 None:intergenic
AGACTCGCCGTCTCCGGCAC+CGG 0.510633 4.3:+33900415 None:intergenic
AGCTGGGAATAAGCATTGGT+TGG 0.510903 4.3:+33899988 None:intergenic
TTCTCAGGCCGATGATGTTG+CGG 0.519029 4.3:-33900109 MS.gene50715:intron
AAAAGAAATTGATGTTTACC+AGG 0.530934 4.3:-33900537 MS.gene50715:CDS
GCTGGGAATAAGCATTGGTT+GGG 0.531676 4.3:+33899989 None:intergenic
GAAAACGTCGGGCTGCATGC+AGG 0.532133 4.3:+33899700 None:intergenic
AAAACGCCACAGTGGAGAGC+TGG 0.532352 4.3:-33899799 MS.gene50715:CDS
AATTGATGTTTACCAGGAGG+AGG 0.533216 4.3:-33900531 MS.gene50715:CDS
TTCCAAATCCGCAACATCAT+CGG 0.540001 4.3:+33900101 None:intergenic
AGCCCGAAGCCCAAACGGGT+GGG 0.541657 4.3:-33899861 MS.gene50715:CDS
GAATAAGCATTGGTTGGGCC+CGG 0.542060 4.3:+33899994 None:intergenic
ATTGTATCCAGTTCAGGCCC+GGG 0.543140 4.3:-33900034 MS.gene50715:CDS
CTGCATGCAGGTCTATACTC+AGG 0.544535 4.3:+33899712 None:intergenic
AGAGAGCTGGGAATAAGCAT+TGG 0.549150 4.3:+33899984 None:intergenic
GCCGAACCGGAACCTAGACC+GGG 0.552879 4.3:-33900012 MS.gene50715:CDS
TCTGCGGATTATAGAGAGCT+GGG 0.553907 4.3:+33899972 None:intergenic
CATTTGAATCCGGTTCAGAC+CGG 0.555998 4.3:+33899603 None:intergenic
GCTGTTCGAATGAATTTCAC+CGG 0.556294 4.3:+33899678 None:intergenic
AAGCCCGAAGCCCAAACGGG+TGG 0.557944 4.3:-33899862 MS.gene50715:CDS
TCCGGCACCGGAAAATGTTG+TGG 0.574032 4.3:+33900427 None:intergenic
TCATTCGAACAGCCACCGTA+AGG 0.576237 4.3:-33899668 MS.gene50715:CDS
ATACTCAGGGAAAAGCCGAC+CGG 0.581357 4.3:+33899726 None:intergenic
CTAGAACAACCAAGACAAGA+AGG 0.581886 4.3:-33899945 MS.gene50715:CDS
ACCATGTTGAAATTCACCGT+TGG 0.582797 4.3:+33900568 None:intergenic
CACCGGAACTGCTAAACCAC+CGG 0.593758 4.3:+33900379 None:intergenic
TGGTTGTTCTAGGCTTTCTG+CGG 0.603627 4.3:+33899956 None:intergenic
TGAATTTCACCGGAAAACGT+CGG 0.605768 4.3:+33899688 None:intergenic
AAACATGGAAGTTGTTGTAG+CGG 0.614309 4.3:-33900720 None:intergenic
TGCATGCAGGTCTATACTCA+GGG 0.615040 4.3:+33899713 None:intergenic
TCGTTCATGTTCAGAACCTG+AGG 0.628083 4.3:+33900475 None:intergenic
TTCATGTTCAGAACCTGAGG+TGG 0.629594 4.3:+33900478 None:intergenic
GTGTTCGGTTTAAGTCCGGT+CGG 0.631814 4.3:-33899741 MS.gene50715:CDS
TCCAACGGTGAATTTCAACA+TGG 0.652718 4.3:-33900569 MS.gene50715:CDS
GAAGGCCCAACTGTAACGTG+TGG 0.654146 4.3:-33899927 MS.gene50715:CDS
AAAACACTATGTAACGCTTG+TGG 0.656575 4.3:-33899763 MS.gene50715:CDS
AGAAATTGATGTTTACCAGG+AGG 0.667546 4.3:-33900534 MS.gene50715:CDS
AAGCGTTGAAACCAAGCTTG+AGG 0.681951 4.3:-33900697 MS.gene50715:CDS
TCATTTCCAAAACCTTACGG+TGG 0.687073 4.3:+33899656 None:intergenic
AGCGGACCAGCTCTCCACTG+TGG 0.697012 4.3:+33899793 None:intergenic
ATGTTCAGAAAACGCCACAG+TGG 0.715931 4.3:-33899807 MS.gene50715:CDS
GTGGGGCTCAGTTTCAACGA+CGG 0.724625 4.3:-33899843 MS.gene50715:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAGAAATTGATGTTTACC+AGG - chr4.3:33899770-33899789 MS.gene50715:CDS 25.0%
!!! TTTTGGTTTTCTTAATTCTC+AGG - chr4.3:33900183-33900202 MS.gene50715:intron 25.0%
AATTCATTACCTTTGTAGCA+TGG - chr4.3:33900002-33900021 MS.gene50715:CDS 30.0%
TCTCTTCACAAATTGTTTGT+TGG + chr4.3:33899650-33899669 None:intergenic 30.0%
! TCAAATTCCACAACATTTTC+CGG - chr4.3:33899873-33899892 MS.gene50715:CDS 30.0%
! TCATAGCTAATAACTCGTTT+TGG - chr4.3:33900166-33900185 MS.gene50715:intron 30.0%
! TCTTTGCTGAAAAGAGTATA+GGG + chr4.3:33900414-33900433 None:intergenic 30.0%
! TTCTTTGCTGAAAAGAGTAT+AGG + chr4.3:33900415-33900434 None:intergenic 30.0%
AAAAATCCACACGTTACAGT+TGG + chr4.3:33900389-33900408 None:intergenic 35.0%
AAAACACTATGTAACGCTTG+TGG - chr4.3:33900544-33900563 MS.gene50715:CDS 35.0%
AAAATCCACACGTTACAGTT+GGG + chr4.3:33900388-33900407 None:intergenic 35.0%
AGAAATTGATGTTTACCAGG+AGG - chr4.3:33899773-33899792 MS.gene50715:CDS 35.0%
TATCATTAGTGTCATGTGTC+CGG - chr4.3:33900114-33900133 MS.gene50715:intron 35.0%
TTGCATGATTAAAGACTCAC+CGG + chr4.3:33899948-33899967 None:intergenic 35.0%
! CAAGCGTTACATAGTGTTTT+CGG + chr4.3:33900544-33900563 None:intergenic 35.0%
! GAATTTCAACATGGTTCTGT+TGG - chr4.3:33899747-33899766 MS.gene50715:CDS 35.0%
!! GACTTGCTTGATTTTTCCAA+CGG - chr4.3:33899723-33899742 MS.gene50715:CDS 35.0%
AACGCAAACAACGTTGTTGT+CGG - chr4.3:33899681-33899700 MS.gene50715:CDS 40.0%
AACTCGTGACCATTTGAATC+CGG + chr4.3:33900717-33900736 None:intergenic 40.0%
AATTGATGTTTACCAGGAGG+AGG - chr4.3:33899776-33899795 MS.gene50715:CDS 40.0%
ACCATGTTGAAATTCACCGT+TGG + chr4.3:33899742-33899761 None:intergenic 40.0%
ATAGATTCTGTCCTCAAGCT+TGG + chr4.3:33899624-33899643 None:intergenic 40.0%
ATGATGTTGCGGATTTGGAA+TGG - chr4.3:33900209-33900228 MS.gene50715:intron 40.0%
CAGAAATGTCCATGCTACAA+AGG + chr4.3:33900014-33900033 None:intergenic 40.0%
CTAGAACAACCAAGACAAGA+AGG - chr4.3:33900362-33900381 MS.gene50715:intron 40.0%
CTCTCTATTGTATCCAGTTC+AGG - chr4.3:33900267-33900286 MS.gene50715:intron 40.0%
CTTCTTGTCTTGGTTGTTCT+AGG + chr4.3:33900364-33900383 None:intergenic 40.0%
GCCTCATTTCCAAAACCTTA+CGG + chr4.3:33900657-33900676 None:intergenic 40.0%
GCTGTTCGAATGAATTTCAC+CGG + chr4.3:33900632-33900651 None:intergenic 40.0%
GGATACAATAGAGAGAGTTC+TGG + chr4.3:33900262-33900281 None:intergenic 40.0%
TATGTAACGCTTGTGGTGTT+CGG - chr4.3:33900551-33900570 MS.gene50715:CDS 40.0%
TCATTTCCAAAACCTTACGG+TGG + chr4.3:33900654-33900673 None:intergenic 40.0%
TCCAACGGTGAATTTCAACA+TGG - chr4.3:33899738-33899757 MS.gene50715:CDS 40.0%
TGAATTTCACCGGAAAACGT+CGG + chr4.3:33900622-33900641 None:intergenic 40.0%
TTCCAAATCCGCAACATCAT+CGG + chr4.3:33900209-33900228 None:intergenic 40.0%
! TCTTTTCAGCAAAGAAGCAG+AGG - chr4.3:33900419-33900438 MS.gene50715:CDS 40.0%
! TGATGTTGCGGATTTGGAAT+GGG - chr4.3:33900210-33900229 MS.gene50715:intron 40.0%
!!! ACCGTAAGGTTTTGGAAATG+AGG - chr4.3:33900653-33900672 MS.gene50715:CDS 40.0%
!!! GTAAGGTTTTGGAAATGAGG+CGG - chr4.3:33900656-33900675 MS.gene50715:CDS 40.0%
AGAGAGCTGGGAATAAGCAT+TGG + chr4.3:33900326-33900345 None:intergenic 45.0%
ATGTTCAGAAAACGCCACAG+TGG - chr4.3:33900500-33900519 MS.gene50715:CDS 45.0%
ATTAGTGTCATGTGTCCGGT+CGG - chr4.3:33900118-33900137 MS.gene50715:intron 45.0%
CATTTGAATCCGGTTCAGAC+CGG + chr4.3:33900707-33900726 None:intergenic 45.0%
GAAAAAAACAGCACCACCTC+AGG - chr4.3:33899816-33899835 MS.gene50715:CDS 45.0%
GAATTTCACCGGAAAACGTC+GGG + chr4.3:33900621-33900640 None:intergenic 45.0%
TATTGTATCCAGTTCAGGCC+CGG - chr4.3:33900272-33900291 MS.gene50715:intron 45.0%
TCGTTCATGTTCAGAACCTG+AGG + chr4.3:33899835-33899854 None:intergenic 45.0%
TGCATGCAGGTCTATACTCA+GGG + chr4.3:33900597-33900616 None:intergenic 45.0%
TGCTAAACCACCGGCAAAAA+TGG + chr4.3:33899922-33899941 None:intergenic 45.0%
TGTGGTGTTCGGTTTAAGTC+CGG - chr4.3:33900562-33900581 MS.gene50715:CDS 45.0%
TTCATGTTCAGAACCTGAGG+TGG + chr4.3:33899832-33899851 None:intergenic 45.0%
! ACTGTGGCGTTTTCTGAACA+TGG + chr4.3:33900501-33900520 None:intergenic 45.0%
! CATAGTGTTTTCGGTCCAAG+CGG + chr4.3:33900535-33900554 None:intergenic 45.0%
! TCTGCGGATTATAGAGAGCT+GGG + chr4.3:33900338-33900357 None:intergenic 45.0%
! TGGTTGTTCTAGGCTTTCTG+CGG + chr4.3:33900354-33900373 None:intergenic 45.0%
! TTCTGCGGATTATAGAGAGC+TGG + chr4.3:33900339-33900358 None:intergenic 45.0%
!! AAGCGTTGAAACCAAGCTTG+AGG - chr4.3:33899610-33899629 MS.gene50715:CDS 45.0%
!! AGCTGGGAATAAGCATTGGT+TGG + chr4.3:33900322-33900341 None:intergenic 45.0%
!! GAGTCTGACTCCATTTTTGC+CGG - chr4.3:33899909-33899928 MS.gene50715:CDS 45.0%
!! GCTGGGAATAAGCATTGGTT+GGG + chr4.3:33900321-33900340 None:intergenic 45.0%
AAAAAGCCCGAAGCCCAAAC+GGG - chr4.3:33900442-33900461 MS.gene50715:CDS 50.0%
ACAGTTGGGCCTTCTTGTCT+TGG + chr4.3:33900374-33900393 None:intergenic 50.0%
ATACTCAGGGAAAAGCCGAC+CGG + chr4.3:33900584-33900603 None:intergenic 50.0%
ATTGTATCCAGTTCAGGCCC+GGG - chr4.3:33900273-33900292 MS.gene50715:intron 50.0%
CGGTCTGAACCGGATTCAAA+TGG - chr4.3:33900705-33900724 MS.gene50715:CDS 50.0%
CTGCATGCAGGTCTATACTC+AGG + chr4.3:33900598-33900617 None:intergenic 50.0%
CTTCTTCTTCTTCCTCCTCC+TGG + chr4.3:33899791-33899810 None:intergenic 50.0%
GAAAAAGCCCGAAGCCCAAA+CGG - chr4.3:33900441-33900460 MS.gene50715:CDS 50.0%
GGAATGGGTGTCTCACTTTG+TGG - chr4.3:33900225-33900244 MS.gene50715:intron 50.0%
GTGTTCGGTTTAAGTCCGGT+CGG - chr4.3:33900566-33900585 MS.gene50715:CDS 50.0%
TCATTCGAACAGCCACCGTA+AGG - chr4.3:33900639-33900658 MS.gene50715:CDS 50.0%
TTCTCAGGCCGATGATGTTG+CGG - chr4.3:33900198-33900217 MS.gene50715:intron 50.0%
TTTGGAAATGAGGCGGAGGA+AGG - chr4.3:33900663-33900682 MS.gene50715:CDS 50.0%
! GAACAGCCACCGTAAGGTTT+TGG - chr4.3:33900645-33900664 MS.gene50715:CDS 50.0%
! GCGTTTTCTGAACATGGCAG+TGG + chr4.3:33900495-33900514 None:intergenic 50.0%
! TCCACAACATTTTCCGGTGC+CGG - chr4.3:33899879-33899898 MS.gene50715:CDS 50.0%
!! GAATAAGCATTGGTTGGGCC+CGG + chr4.3:33900316-33900335 None:intergenic 50.0%
!! TCTGACTCCATTTTTGCCGG+TGG - chr4.3:33899912-33899931 MS.gene50715:CDS 50.0%
!!! AGGTTTTGGAAATGAGGCGG+AGG - chr4.3:33900659-33900678 MS.gene50715:CDS 50.0%
AAAACGCCACAGTGGAGAGC+TGG - chr4.3:33900508-33900527 MS.gene50715:CDS 55.0%
AAACTGAGCCCCACCCGTTT+GGG + chr4.3:33900458-33900477 None:intergenic 55.0%
ATCCGGTTCAGACCGGAAAC+CGG + chr4.3:33900700-33900719 None:intergenic 55.0%
CACCGGAACTGCTAAACCAC+CGG + chr4.3:33899931-33899950 None:intergenic 55.0%
GAAGGCCCAACTGTAACGTG+TGG - chr4.3:33900380-33900399 MS.gene50715:intron 55.0%
GACACGACACAAACACCGAC+CGG + chr4.3:33900136-33900155 None:intergenic 55.0%
GAGGAAGGAGGTGGATGAAC+CGG - chr4.3:33900678-33900697 MS.gene50715:CDS 55.0%
GGCCGATGATGTTGCGGATT+TGG - chr4.3:33900204-33900223 MS.gene50715:intron 55.0%
GTGGGGCTCAGTTTCAACGA+CGG - chr4.3:33900464-33900483 MS.gene50715:CDS 55.0%
TGCCGGTGGTTTAGCAGTTC+CGG - chr4.3:33899926-33899945 MS.gene50715:CDS 55.0%
! AACCGGTTTCCGGTCTGAAC+CGG - chr4.3:33900695-33900714 MS.gene50715:CDS 55.0%
! GAGGTGGATGAACCGGTTTC+CGG - chr4.3:33900685-33900704 MS.gene50715:CDS 55.0%
! TCCGGCACCGGAAAATGTTG+TGG + chr4.3:33899883-33899902 None:intergenic 55.0%
!! ACATTTTCCGGTGCCGGAGA+CGG - chr4.3:33899885-33899904 MS.gene50715:CDS 55.0%
AATGAGGCGGAGGAAGGAGG+TGG - chr4.3:33900669-33900688 MS.gene50715:CDS 60.0%
GAAAACGTCGGGCTGCATGC+AGG + chr4.3:33900610-33900629 None:intergenic 60.0%
GAAACTGAGCCCCACCCGTT+TGG + chr4.3:33900459-33900478 None:intergenic 60.0%
GGAAATGAGGCGGAGGAAGG+AGG - chr4.3:33900666-33900685 MS.gene50715:CDS 60.0%
! GCATGCAGCCCGACGTTTTC+CGG - chr4.3:33900610-33900629 MS.gene50715:CDS 60.0%
AAGCCCGAAGCCCAAACGGG+TGG - chr4.3:33900445-33900464 MS.gene50715:CDS 65.0%
AGCCCCACCCGTTTGGGCTT+CGG + chr4.3:33900452-33900471 None:intergenic 65.0%
AGCCCGAAGCCCAAACGGGT+GGG - chr4.3:33900446-33900465 MS.gene50715:CDS 65.0%
AGCGGACCAGCTCTCCACTG+TGG + chr4.3:33900517-33900536 None:intergenic 65.0%
CAGTGGAGAGCTGGTCCGCT+TGG - chr4.3:33900517-33900536 MS.gene50715:CDS 65.0%
GCCCGGTCTAGGTTCCGGTT+CGG + chr4.3:33900299-33900318 None:intergenic 65.0%
GCCGAACCGGAACCTAGACC+GGG - chr4.3:33900295-33900314 MS.gene50715:intron 65.0%
GGAGTCAGACTCGCCGTCTC+CGG + chr4.3:33899901-33899920 None:intergenic 65.0%
GGCCGAACCGGAACCTAGAC+CGG - chr4.3:33900294-33900313 MS.gene50715:intron 65.0%
GTTGGGCCCGGTCTAGGTTC+CGG + chr4.3:33900304-33900323 None:intergenic 65.0%
!! GCATTGGTTGGGCCCGGTCT+AGG + chr4.3:33900310-33900329 None:intergenic 65.0%
!! GTCTAGGTTCCGGTTCGGCC+CGG + chr4.3:33900294-33900313 None:intergenic 65.0%
!! TCTAGGTTCCGGTTCGGCCC+GGG + chr4.3:33900293-33900312 None:intergenic 65.0%
AGACTCGCCGTCTCCGGCAC+CGG + chr4.3:33899895-33899914 None:intergenic 70.0%
AGTTCAGGCCCGGGCCGAAC+CGG - chr4.3:33900282-33900301 MS.gene50715:intron 70.0%
GCCCCACCCGTTTGGGCTTC+GGG + chr4.3:33900451-33900470 None:intergenic 70.0%
GCCCGAAGCCCAAACGGGTG+GGG - chr4.3:33900447-33900466 MS.gene50715:CDS 70.0%
GGTTCGGCCCGGGCCTGAAC+TGG + chr4.3:33900283-33900302 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 33899591 33900738 33899591 ID=MS.gene50715
chr4.3 mRNA 33899591 33900738 33899591 ID=MS.gene50715.t1;Parent=MS.gene50715
chr4.3 exon 33900382 33900738 33900382 ID=MS.gene50715.t1.exon1;Parent=MS.gene50715.t1
chr4.3 CDS 33900382 33900738 33900382 ID=cds.MS.gene50715.t1;Parent=MS.gene50715.t1
chr4.3 exon 33899591 33900124 33899591 ID=MS.gene50715.t1.exon2;Parent=MS.gene50715.t1
chr4.3 CDS 33899591 33900124 33899591 ID=cds.MS.gene50715.t1;Parent=MS.gene50715.t1
Gene Sequence

>MS.gene50715

ATGGAAGTTGTTGTAGCGGAAGCGTTGAAACCAAGCTTGAGGACAGAATCTATTTTCCAACAAACAATTTGTGAAGAGATTTTGTGTTTGAACGCAAACAACGTTGTTGTCGGAGAAGATTTTTCTGTCGACGACTTGCTTGATTTTTCCAACGGTGAATTTCAACATGGTTCTGTTGGAAAAGAAATTGATGTTTACCAGGAGGAGGAAGAAGAAGAAGAACATGAAAAAAACAGCACCACCTCAGGTTCTGAACATGAACGAACAGAAGATGACAACAATTCAAATTCCACAACATTTTCCGGTGCCGGAGACGGCGAGTCTGACTCCATTTTTGCCGGTGGTTTAGCAGTTCCGGTGAGTCTTTAATCATGCAATAAATTATTTTTGTTTTTTATTTAATAAAGAATGAATTCATTACCTTTGTAGCATGGACATTTCTGATAGAAGATGTGCTAGCATGCGACACATGTCACTAACACGACACATTTGATTACATTGAATTATGTTATTTTTTTTGTATTATCATTAGTGTCATGTGTCCGGTCGGTGTTTGTGTCGTGTCTGTATGTGCTTCATAGCTAATAACTCGTTTTGGTTTTCTTAATTCTCAGGCCGATGATGTTGCGGATTTGGAATGGGTGTCTCACTTTGTGGACGATTCTGTACCAGAACTCTCTCTATTGTATCCAGTTCAGGCCCGGGCCGAACCGGAACCTAGACCGGGCCCAACCAATGCTTATTCCCAGCTCTCTATAATCCGCAGAAAGCCTAGAACAACCAAGACAAGAAGGCCCAACTGTAACGTGTGGATTTTTAACCCTATACTCTTTTCAGCAAAGAAGCAGAGGAAAAAGCCCGAAGCCCAAACGGGTGGGGCTCAGTTTCAACGACGGTGCAGCCACTGCCATGTTCAGAAAACGCCACAGTGGAGAGCTGGTCCGCTTGGACCGAAAACACTATGTAACGCTTGTGGTGTTCGGTTTAAGTCCGGTCGGCTTTTCCCTGAGTATAGACCTGCATGCAGCCCGACGTTTTCCGGTGAAATTCATTCGAACAGCCACCGTAAGGTTTTGGAAATGAGGCGGAGGAAGGAGGTGGATGAACCGGTTTCCGGTCTGAACCGGATTCAAATGGTCACGAGTTGA
Protein sequence

>MS.gene50715.t1

MEVVVAEALKPSLRTESIFQQTICEEILCLNANNVVVGEDFSVDDLLDFSNGEFQHGSVGKEIDVYQEEEEEEEHEKNSTTSGSEHERTEDDNNSNSTTFSGAGDGESDSIFAGGLAVPADDVADLEWVSHFVDDSVPELSLLYPVQARAEPEPRPGPTNAYSQLSIIRRKPRTTKTRRPNCNVWIFNPILFSAKKQRKKPEAQTGGAQFQRRCSHCHVQKTPQWRAGPLGPKTLCNACGVRFKSGRLFPEYRPACSPTFSGEIHSNSHRKVLEMRRRKEVDEPVSGLNRIQMVTS