AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50761

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50761.t1 MTR_6g011450 82.432 518 76 4 29 531 309 826 0.0 805
MS.gene50761.t1 MTR_6g011450 82.432 518 76 4 29 531 171 688 0.0 803
MS.gene50761.t1 MTR_4g073340 79.520 542 65 5 1 531 1 507 0.0 770
MS.gene50761.t1 MTR_8g086050 85.985 264 15 3 566 816 370 624 1.56e-155 468
MS.gene50761.t1 MTR_8g086050 86.312 263 14 3 566 815 370 623 3.35e-155 471
MS.gene50761.t1 MTR_8g085680 76.046 263 41 3 566 815 369 622 3.46e-136 421
MS.gene50761.t1 MTR_8g085680 76.046 263 41 3 566 815 369 622 5.54e-136 421
MS.gene50761.t1 MTR_8g086050 84.848 231 13 2 566 783 370 591 1.24e-130 404
MS.gene50761.t1 MTR_6g011470 71.837 245 33 3 275 518 1 210 1.97e-112 341
MS.gene50761.t1 MTR_8g085680 77.348 181 28 1 566 733 369 549 5.94e-90 296
MS.gene50761.t1 MTR_2g056120 53.409 264 101 4 566 816 366 620 8.88e-88 293
MS.gene50761.t1 MTR_7g103840 33.526 173 110 3 352 521 846 1016 6.27e-30 128
MS.gene50761.t1 MTR_7g103840 33.526 173 110 3 352 521 848 1018 8.05e-30 127
MS.gene50761.t1 MTR_1g061130 34.104 173 107 4 352 519 839 1009 2.72e-28 122
MS.gene50761.t1 MTR_1g061130 34.104 173 107 4 352 519 839 1009 3.15e-28 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50761.t1 AT1G55320 68.821 263 60 4 566 815 150 403 3.00e-122 378
MS.gene50761.t1 AT1G55320 68.441 263 61 3 566 815 366 619 2.82e-119 377
MS.gene50761.t1 AT4G04885 68.545 213 63 2 320 531 591 800 8.83e-92 306
MS.gene50761.t1 AT4G04885 51.111 90 36 3 39 125 303 387 9.25e-16 82.4
MS.gene50761.t1 AT5G23050 48.864 264 113 4 566 816 361 615 1.89e-78 268
MS.gene50761.t1 AT5G23050 47.210 233 101 4 566 785 361 584 5.62e-64 225
MS.gene50761.t1 AT1G66500 35.000 360 168 14 200 524 66 394 3.83e-45 168
MS.gene50761.t1 AT5G43620 36.111 288 134 10 269 524 119 388 2.84e-42 159
MS.gene50761.t1 AT2G36480 32.941 170 106 3 352 521 786 947 1.60e-23 107
MS.gene50761.t1 AT2G36480 32.941 170 106 3 352 521 786 947 1.60e-23 107

Find 225 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTGTATGCAGGTTATTT+TGG 0.133360 4.4:+35231660 MS.gene50761:intron
CTATTGCTGGCCTACAAATT+TGG 0.134649 4.4:+35230524 MS.gene50761:CDS
CCATGATGAGGTTTACTTTA+AGG 0.136545 4.4:+35232530 MS.gene50761:CDS
GGATGCAGGTGTTACCATTT+TGG 0.185825 4.4:+35231505 MS.gene50761:intron
CAATTAGAGCTCTTGGATTT+TGG 0.198744 4.4:-35225461 None:intergenic
TACATAATGCACATGTATTT+TGG 0.206485 4.4:-35226844 None:intergenic
AAACAGCTCTTTCAGTTTCC+TGG 0.223690 4.4:+35226211 MS.gene50761:CDS
CCCCTGCGGCCTCAAGCTTT+TGG 0.226216 4.4:+35231795 MS.gene50761:CDS
TCTCCTCAAGGCCATGGTTT+TGG 0.228125 4.4:+35230618 MS.gene50761:CDS
TTATATCATTGCTTTCTAAA+TGG 0.249459 4.4:+35230570 MS.gene50761:CDS
TGCGAGAGGGCTTCTGCTTA+TGG 0.258664 4.4:-35226688 None:intergenic
ACGCGGAGCATGCACATTTA+TGG 0.263678 4.4:-35225814 None:intergenic
CATCAAGAGCACTTCGTATT+GGG 0.284804 4.4:-35230467 None:intergenic
CATGATGAGGTTTACTTTAA+GGG 0.286532 4.4:+35232531 MS.gene50761:CDS
TTATTCCATTAGGCGTATTA+AGG 0.289551 4.4:-35226931 None:intergenic
GCACTTGCTGCAACGGTAAC+AGG 0.291227 4.4:-35225699 None:intergenic
TGACGAACCTTCAGAAGATT+TGG 0.298615 4.4:-35226533 None:intergenic
AAGAGCTGTTTGCAATTCTT+TGG 0.300209 4.4:-35226199 None:intergenic
GCTTACTGCCAGAAGTTTCT+TGG 0.305569 4.4:-35225506 None:intergenic
GCTTGGAACGGTTCCCAAAA+TGG 0.305618 4.4:-35231519 None:intergenic
ATGTGTATTAGCAGGTTCTT+AGG 0.307494 4.4:+35232955 MS.gene50761:intron
ATGCCTGTTGTTATTCCATT+AGG 0.318753 4.4:-35226941 None:intergenic
GGTTTATTCCCTGTCTCTCT+TGG 0.324602 4.4:+35232480 MS.gene50761:CDS
ATTTATTCCACCAATGTTCA+TGG 0.328257 4.4:-35233045 None:intergenic
TATTGCTGGCCTACAAATTT+GGG 0.330034 4.4:+35230525 MS.gene50761:CDS
AGAAACCATTACTTCTACTA+TGG 0.330076 4.4:-35225644 None:intergenic
CCTCCACATAATTCAATGAT+TGG 0.330215 4.4:-35231738 None:intergenic
ATAACTGATGAAGAATTCTT+TGG 0.332236 4.4:-35225375 None:intergenic
ATGCATTTAGCATGAATAAT+GGG 0.333257 4.4:-35226983 None:intergenic
GATTCGAATGGATTCCTAAC+AGG 0.333615 4.4:-35225873 None:intergenic
ACAAATTTGGGATGGGTGAT+AGG 0.340700 4.4:+35230537 MS.gene50761:CDS
CGAGTCCAAGGAATAGCTTT+TGG 0.340865 4.4:-35230438 None:intergenic
TCTAGGAAGATCGCCATATA+AGG 0.342211 4.4:-35226573 None:intergenic
TCTGCAATCAGTGCCTTATA+TGG 0.351640 4.4:+35226560 MS.gene50761:CDS
GGTTTCTTGCAACCAACTAT+TGG 0.360158 4.4:+35225660 MS.gene50761:CDS
TTCCAAGCTTAGTGAAATCT+TGG 0.364156 4.4:+35231534 MS.gene50761:CDS
TATGCATTTAGCATGAATAA+TGG 0.367648 4.4:-35226984 None:intergenic
GGTAGAGCATGTGGGCCAGC+TGG 0.367975 4.4:-35226257 None:intergenic
AACAGCTCTTTCAGTTTCCT+GGG 0.369668 4.4:+35226212 MS.gene50761:CDS
TGGCAGTAAGCCTTTGTTAG+TGG 0.376982 4.4:+35225518 MS.gene50761:CDS
TCAAAAGTTGCTGGCAGAAC+TGG 0.381258 4.4:-35225780 None:intergenic
TAGGCATCAATTAGAGCTCT+TGG 0.388106 4.4:-35225468 None:intergenic
TGTGACTTCTGTGCTCATCT+TGG 0.389046 4.4:-35226628 None:intergenic
GGCATAACAAGTTGTGCATT+TGG 0.389349 4.4:-35226161 None:intergenic
TTCCAAGATTTCACTAAGCT+TGG 0.392624 4.4:-35231536 None:intergenic
GGGACTTCCCGTAATGTAAC+TGG 0.392704 4.4:-35231775 None:intergenic
GCTGATTAGGCAACTGATAC+AGG 0.398235 4.4:-35225995 None:intergenic
AAGGGAATGCCCTTGCATAA+AGG 0.411507 4.4:+35232549 MS.gene50761:CDS
TAACAAGTTGTGCATTTGGC+AGG 0.415660 4.4:-35226157 None:intergenic
GACAAAAGCCAAGAAACTTC+TGG 0.416293 4.4:+35225498 MS.gene50761:CDS
TTGCCACCTTTACCTCCAGC+TGG 0.419948 4.4:+35226242 MS.gene50761:CDS
AAGTTGGGGATGTCTATTGC+TGG 0.420616 4.4:+35230511 MS.gene50761:CDS
GTGACTTCTGTGCTCATCTT+GGG 0.422924 4.4:-35226627 None:intergenic
GATTGGGATTAGGTAGAATC+TGG 0.424134 4.4:-35226280 None:intergenic
AATCCAATCATTGAATTATG+TGG 0.424565 4.4:+35231735 MS.gene50761:CDS
AAAGAAAGAAGATGAAGAGT+TGG 0.425274 4.4:+35226802 MS.gene50761:CDS
CCATCAAGAGCACTTCGTAT+TGG 0.425421 4.4:-35230468 None:intergenic
CTGCTATTGACATTCAAGTT+GGG 0.430087 4.4:+35230496 MS.gene50761:CDS
GGATTCTTCGATGGAAATAT+AGG 0.431337 4.4:-35225852 None:intergenic
CTGTTTGCAATTCTTTGGCC+TGG 0.431824 4.4:-35226194 None:intergenic
CCAATACGAAGTGCTCTTGA+TGG 0.432832 4.4:+35230468 MS.gene50761:CDS
GAAGGACCAGGATTGGGATT+AGG 0.436097 4.4:-35226290 None:intergenic
GTGGACAAACGCCACCATTC+CGG 0.438771 4.4:+35226051 MS.gene50761:CDS
CTCCAAAAGCTTGAGGCCGC+AGG 0.443253 4.4:-35231797 None:intergenic
GTGGCTAGGTGCAGAGGCAT+TGG 0.444504 4.4:+35226733 MS.gene50761:CDS
TTCATCTTGGTGGGAATACT+CGG 0.444524 4.4:+35225343 MS.gene50761:CDS
GCTTGCGTCCAGTTACATTA+CGG 0.444712 4.4:+35231767 MS.gene50761:CDS
GACACAGAAGGACCAGGATT+GGG 0.445177 4.4:-35226296 None:intergenic
GCGTTTGTCCACTGTTATGC+GGG 0.451893 4.4:-35226040 None:intergenic
GTATACCTTAATACGCCTAA+TGG 0.453857 4.4:+35226926 MS.gene50761:CDS
CTTGCGTCCAGTTACATTAC+GGG 0.455274 4.4:+35231768 MS.gene50761:CDS
CGCCTCGTTTCAAAAGTTGC+TGG 0.461501 4.4:-35225789 None:intergenic
TAAGTAAATTGTGACGCTGC+TGG 0.462016 4.4:-35226092 None:intergenic
TTCCATCGAAGAATCCTGTT+AGG 0.463431 4.4:+35225859 MS.gene50761:CDS
GCTCATCTTGGGACTTGAAT+CGG 0.465641 4.4:-35226616 None:intergenic
CTTTGGCCTGGCGAAGGAAG+TGG 0.465990 4.4:-35226182 None:intergenic
TTGGGAGCTGAATCAGCTCC+AGG 0.466055 4.4:+35226752 MS.gene50761:CDS
TCAGATGCTCCAAGAGAGAC+AGG 0.473039 4.4:-35232489 None:intergenic
ATCACCCATCCCAAATTTGT+AGG 0.473339 4.4:-35230534 None:intergenic
TCACTGTAAAACTTGTCAAA+AGG 0.474303 4.4:-35226872 None:intergenic
GCCTTTGTTAGTGGAGCGAC+CGG 0.474907 4.4:+35225527 MS.gene50761:CDS
GATATAATCAAGAGAATGGT+TGG 0.477992 4.4:+35232987 MS.gene50761:CDS
GGCCCAAGGTGTTATGTCAT+TGG 0.480316 4.4:+35226367 MS.gene50761:CDS
CCATGTTCATGCACAGGAAA+AGG 0.480750 4.4:-35225942 None:intergenic
TCTGATGAGAAACCAATAGT+TGG 0.483041 4.4:-35225672 None:intergenic
ACTTATAGCACTTCTTTGCC+AGG 0.484285 4.4:+35226110 MS.gene50761:CDS
GCATCTCTTTCTTTGTACCA+TGG 0.485412 4.4:+35230594 MS.gene50761:CDS
AGCACAGCATCAATGACAAC+TGG 0.485665 4.4:+35231825 MS.gene50761:CDS
TGTGGGCCAGCTGGAGGTAA+AGG 0.486896 4.4:-35226248 None:intergenic
ACGCCTAATGGAATAACAAC+AGG 0.488809 4.4:+35226938 MS.gene50761:CDS
GAGATCCAAAAGCTATTCCT+TGG 0.489267 4.4:+35230433 MS.gene50761:CDS
TTAGCATTTATTGATTCGAA+TGG 0.491940 4.4:-35225885 None:intergenic
ATAGATTTAGTTACCAGCGC+AGG 0.494952 4.4:-35226401 None:intergenic
TGGCTAGGTGCAGAGGCATT+GGG 0.497013 4.4:+35226734 MS.gene50761:CDS
TACGAAGTGCTCTTGATGGA+TGG 0.497932 4.4:+35230472 MS.gene50761:CDS
TGACACAGAAGGACCAGGAT+TGG 0.501011 4.4:-35226297 None:intergenic
ATCAGACCGGGTAGCTGATT+AGG 0.502180 4.4:-35226008 None:intergenic
CTGAAGAGGAGGAGTTCGAT+TGG 0.502985 4.4:+35225595 MS.gene50761:CDS
GGCATGGACAGGTCGCAGTT+AGG 0.505187 4.4:+35226959 MS.gene50761:CDS
CCACAAGTTGTGCACTGTCT+AGG 0.505428 4.4:-35226590 None:intergenic
TCATCAAAACATCTCTTTCG+CGG 0.506613 4.4:-35225408 None:intergenic
TAATTTGATTAGTTCACTCA+TGG 0.508846 4.4:+35226346 MS.gene50761:CDS
GTTCCTTGTGTTGGTGAAGT+GGG 0.510523 4.4:+35232459 MS.gene50761:CDS
ACTATGATTGTTCAGGATGC+AGG 0.510990 4.4:+35231491 MS.gene50761:intron
ACAGAGGATTGGATGTATAG+AGG 0.511772 4.4:+35226899 MS.gene50761:CDS
CTAGAAAATGTTCCTTGTGT+TGG 0.513511 4.4:+35232450 MS.gene50761:CDS
AGAGCTCTAATTGATGCCTA+TGG 0.514260 4.4:+35225471 MS.gene50761:CDS
AGCCAATGACATAACACCTT+GGG 0.518658 4.4:-35226369 None:intergenic
TGGAAATATAGGAGTTATGC+TGG 0.520072 4.4:-35225841 None:intergenic
GCTGCTATTGACATTCAAGT+TGG 0.520509 4.4:+35230495 MS.gene50761:CDS
TGGAGATATAATCAAGAGAA+TGG 0.526748 4.4:+35232983 MS.gene50761:CDS
ACAGTGATGAAACAGAGGAT+TGG 0.526805 4.4:+35226888 MS.gene50761:CDS
CCATCACAAAATATTCAGAC+GGG 0.528028 4.4:-35227018 None:intergenic
AGCACAGAAGTCACATGGAT+TGG 0.529243 4.4:+35226636 MS.gene50761:CDS
TGCTATTGACATTCAAGTTG+GGG 0.532401 4.4:+35230497 MS.gene50761:CDS
TCCAAAAGCTTGAGGCCGCA+GGG 0.534212 4.4:-35231796 None:intergenic
ATTTCATATCAGGAGGTGGC+AGG 0.534414 4.4:+35229617 MS.gene50761:CDS
CAGTATTTCATATCAGGAGG+TGG 0.535282 4.4:+35229613 MS.gene50761:CDS
TGAAGAGGAGGAGTTCGATT+GGG 0.536389 4.4:+35225596 MS.gene50761:CDS
CTCATAGCAGCACAATGTCC+TGG 0.542263 4.4:-35226128 None:intergenic
GGCTAATCAAGCCCCTGCGC+TGG 0.542720 4.4:+35226388 MS.gene50761:CDS
AACAAGTTGTGCATTTGGCA+GGG 0.544007 4.4:-35226156 None:intergenic
AATATCCAACAGTTGGCTGT+TGG 0.544187 4.4:-35226322 None:intergenic
AAATTAGAATATCCAACAGT+TGG 0.545058 4.4:-35226329 None:intergenic
TGCAACTACTTCAGCGTCAA+GGG 0.545212 4.4:+35225719 MS.gene50761:CDS
GAATAACAACAGGCATGGAC+AGG 0.551052 4.4:+35226948 MS.gene50761:CDS
GCAATTCTTTGGCCTGGCGA+AGG 0.555122 4.4:-35226188 None:intergenic
GTTATGCCACTTCCTTCGCC+AGG 0.555543 4.4:+35226176 MS.gene50761:CDS
TAGATTTAGTTACCAGCGCA+GGG 0.556762 4.4:-35226400 None:intergenic
ACAAGTTGTGCATTTGGCAG+GGG 0.557390 4.4:-35226155 None:intergenic
GGTGGCTATATCATCGTACA+AGG 0.557509 4.4:+35233008 MS.gene50761:CDS
AGATACCTTTCCTTTATGCA+AGG 0.557986 4.4:-35232559 None:intergenic
GGCGTTTGTCCACTGTTATG+CGG 0.558202 4.4:-35226041 None:intergenic
TAGCAGGTTCTTAGGAGACA+TGG 0.558384 4.4:+35232963 MS.gene50761:intron
GTGCAACTACTTCAGCGTCA+AGG 0.559076 4.4:+35225718 MS.gene50761:CDS
AGCAGAAGCCCTCTCGCAAG+TGG 0.560089 4.4:+35226693 MS.gene50761:CDS
AGAATCTGGGGTAGAGCATG+TGG 0.560420 4.4:-35226266 None:intergenic
AAAGTTGCTGGCAGAACTGG+TGG 0.562453 4.4:-35225777 None:intergenic
TTCCTAACAGGATTCTTCGA+TGG 0.563320 4.4:-35225861 None:intergenic
CAAGTTGTGCATTTGGCAGG+GGG 0.563383 4.4:-35226154 None:intergenic
AGTAGTTGCACTTGCTGCAA+CGG 0.563425 4.4:-35225706 None:intergenic
AATCAAAAGATGCTTGCTGA+TGG 0.564765 4.4:+35225318 None:intergenic
GATACCTTTCCTTTATGCAA+GGG 0.565038 4.4:-35232558 None:intergenic
ATTCTACCTAATCCCAATCC+TGG 0.565745 4.4:+35226284 MS.gene50761:CDS
TGGCTTGACACAGAAGGACC+AGG 0.566253 4.4:-35226302 None:intergenic
CAGAAAATCATTATAATGAG+TGG 0.566493 4.4:-35230388 None:intergenic
CTACTATGGTCTACTAGTGT+CGG 0.567210 4.4:-35225630 None:intergenic
GGCTGTTGGCTTGACACAGA+AGG 0.567803 4.4:-35226308 None:intergenic
TCCATCACAAAATATTCAGA+CGG 0.568109 4.4:-35227019 None:intergenic
TCAAGCCAACAGCCAACTGT+TGG 0.568482 4.4:+35226317 MS.gene50761:CDS
GCTGGCCTACAAATTTGGGA+TGG 0.568482 4.4:+35230529 MS.gene50761:CDS
GACGCTGCTGGATCCTGAGA+TGG 0.569203 4.4:-35226080 None:intergenic
CGGGTTAGATGAAATCAGAC+CGG 0.570016 4.4:-35226021 None:intergenic
ATCAAGAGCACTTCGTATTG+GGG 0.570807 4.4:-35230466 None:intergenic
ACGAAGTGCTCTTGATGGAT+GGG 0.573019 4.4:+35230473 MS.gene50761:CDS
GTAGACCATAGTAGAAGTAA+TGG 0.573238 4.4:+35225639 MS.gene50761:CDS
ATTAGTTCACTCATGGCCCA+AGG 0.574805 4.4:+35226353 MS.gene50761:CDS
AAGCAATGATATAACAGAGT+AGG 0.574823 4.4:-35230561 None:intergenic
AATATAGGAGTTATGCTGGT+AGG 0.575043 4.4:-35225837 None:intergenic
CATGGATCTCCTCAAGGCCA+TGG 0.575075 4.4:+35230612 MS.gene50761:CDS
CCTTAAAGTAAACCTCATCA+TGG 0.575288 4.4:-35232530 None:intergenic
TGTTCCTTGTGTTGGTGAAG+TGG 0.576111 4.4:+35232458 MS.gene50761:CDS
GTTTGATCCAAATCTTCTGA+AGG 0.578645 4.4:+35226526 MS.gene50761:CDS
CAGCTCTTTCAGTTTCCTGG+GGG 0.579833 4.4:+35226214 MS.gene50761:CDS
TTTCAGTATTTCATATCAGG+AGG 0.582952 4.4:+35229610 MS.gene50761:CDS
TCCGGTCGCTCCACTAACAA+AGG 0.582967 4.4:-35225528 None:intergenic
GCAGGAAAATCCCCTTGCAC+AGG 0.583936 4.4:+35229635 MS.gene50761:CDS
ACAGCTCTTTCAGTTTCCTG+GGG 0.586349 4.4:+35226213 MS.gene50761:CDS
CCTAGACAGTGCACAACTTG+TGG 0.586792 4.4:+35226590 MS.gene50761:CDS
TCAACACCATGTTCATGCAC+AGG 0.588454 4.4:-35225948 None:intergenic
CTGGCCTACAAATTTGGGAT+GGG 0.589172 4.4:+35230530 MS.gene50761:CDS
GTTGGCAGTTCTTGCTGACG+AGG 0.590256 4.4:+35226820 MS.gene50761:CDS
AAAGTAAACCTCATCATGGT+CGG 0.590749 4.4:-35232526 None:intergenic
TTCCCAAAACCATGGCCTTG+AGG 0.591486 4.4:-35230621 None:intergenic
TTGTACCATGGATCTCCTCA+AGG 0.591857 4.4:+35230606 MS.gene50761:CDS
CTGAATGCTCCAAAAGCTTG+AGG 0.595297 4.4:-35231804 None:intergenic
ATTGGGGCAAGTCGAGTCCA+AGG 0.596687 4.4:-35230450 None:intergenic
GACGATGTGGCTAGGTGCAG+AGG 0.597079 4.4:+35226727 MS.gene50761:CDS
CCAAAAGCTTGAGGCCGCAG+GGG 0.597788 4.4:-35231795 None:intergenic
GGAGTTATGCTGGTAGGACG+CGG 0.599879 4.4:-35225831 None:intergenic
GTGAGTGAGACGATGTGGCT+AGG 0.601495 4.4:+35226719 MS.gene50761:CDS
AGATTTCACTAAGCTTGGAA+CGG 0.603067 4.4:-35231531 None:intergenic
TTTGTCATTCTTGACGACAA+TGG 0.604567 4.4:+35231849 MS.gene50761:CDS
TAATGGAATAACAACAGGCA+TGG 0.604751 4.4:+35226943 MS.gene50761:CDS
AATAGTACCAGTGTGAGTAA+TGG 0.608110 4.4:+35225906 MS.gene50761:CDS
AGAGCATGTGGGCCAGCTGG+AGG 0.611285 4.4:-35226254 None:intergenic
AATTAGACCATTACTCACAC+TGG 0.612003 4.4:-35225913 None:intergenic
CTCACAAACCACTTGCGAGA+GGG 0.612344 4.4:-35226701 None:intergenic
CTATATCATCGTACAAGGGA+GGG 0.614354 4.4:+35233013 MS.gene50761:CDS
GGGTTAGATGAAATCAGACC+GGG 0.617202 4.4:-35226020 None:intergenic
ATTGGGATTAGGTAGAATCT+GGG 0.617573 4.4:-35226279 None:intergenic
AATGCCCTTGCATAAAGGAA+AGG 0.620306 4.4:+35232554 MS.gene50761:CDS
TAGCCAATGACATAACACCT+TGG 0.621234 4.4:-35226370 None:intergenic
CCAATCATTGAATTATGTGG+AGG 0.624312 4.4:+35231738 MS.gene50761:CDS
TGCATTTAGCATGAATAATG+GGG 0.625810 4.4:-35226982 None:intergenic
CATGGCCTTGAGGAGATCCA+TGG 0.627745 4.4:-35230611 None:intergenic
GAACAAATTTCCCAAAACCA+TGG 0.631785 4.4:-35230629 None:intergenic
GCTATATCATCGTACAAGGG+AGG 0.632238 4.4:+35233012 MS.gene50761:CDS
ATGGCAGAATACTGAAGAGG+AGG 0.632607 4.4:+35225584 MS.gene50761:CDS
ACTCACAAACCACTTGCGAG+AGG 0.632815 4.4:-35226702 None:intergenic
GATGACACCATGAACATTGG+TGG 0.633573 4.4:+35233038 MS.gene50761:CDS
AGCTCTTTCAGTTTCCTGGG+GGG 0.634048 4.4:+35226215 MS.gene50761:CDS
AGATGAGCACAGAAGTCACA+TGG 0.637659 4.4:+35226631 MS.gene50761:CDS
GGGCCAGCTGGAGGTAAAGG+TGG 0.639466 4.4:-35226245 None:intergenic
CAGTTGCCTAATCAGCTACC+CGG 0.639744 4.4:+35226002 MS.gene50761:CDS
ATAAGTTTATGACAACATCA+TGG 0.644139 4.4:+35225565 MS.gene50761:CDS
CATCTTGGGACTTGAATCGG+AGG 0.650361 4.4:-35226613 None:intergenic
GCTGATGACACCATGAACAT+TGG 0.650476 4.4:+35233035 MS.gene50761:CDS
GCTGAATGCCGACCATGATG+AGG 0.659320 4.4:+35232518 MS.gene50761:CDS
ATAATCAAGAGAATGGTTGG+TGG 0.659430 4.4:+35232990 MS.gene50761:CDS
ATCATGGCAGAATACTGAAG+AGG 0.665757 4.4:+35225581 MS.gene50761:CDS
AGATTTAGTTACCAGCGCAG+GGG 0.672730 4.4:-35226399 None:intergenic
TCATCTAACCCGCATAACAG+TGG 0.680332 4.4:+35226032 MS.gene50761:CDS
GAATCTGGGGTAGAGCATGT+GGG 0.680646 4.4:-35226265 None:intergenic
CATCACAAAATATTCAGACG+GGG 0.686925 4.4:-35227017 None:intergenic
TTGGGATTAGGTAGAATCTG+GGG 0.686989 4.4:-35226278 None:intergenic
AAACCCACTTCACCAACACA+AGG 0.689168 4.4:-35232462 None:intergenic
TTGCTGGCAGAACTGGTGGA+CGG 0.691595 4.4:-35225773 None:intergenic
GAGTTAAGCTATCTCAACAA+CGG 0.702080 4.4:+35225429 MS.gene50761:CDS
CAGATGCTCCAAGAGAGACA+GGG 0.703099 4.4:-35232488 None:intergenic
CAAATATTGATGATGATCTG+TGG 0.704903 4.4:+35231691 MS.gene50761:CDS
GGTTTGTGAGTGAGACGATG+TGG 0.738502 4.4:+35226714 MS.gene50761:CDS
GTGGCTATATCATCGTACAA+GGG 0.741466 4.4:+35233009 MS.gene50761:CDS
ACATTACGGGAAGTCCCCTG+CGG 0.755485 4.4:+35231781 MS.gene50761:CDS
TTTGCCAACCGAGACCACTG+AGG 0.774441 4.4:+35226778 MS.gene50761:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.4 gene 35225327 35233073 35225327 ID=MS.gene50761
chr4.4 mRNA 35225327 35233073 35225327 ID=MS.gene50761.t1;Parent=MS.gene50761
chr4.4 exon 35225327 35225740 35225327 ID=MS.gene50761.t1.exon1;Parent=MS.gene50761.t1
chr4.4 CDS 35225327 35225740 35225327 ID=cds.MS.gene50761.t1;Parent=MS.gene50761.t1
chr4.4 exon 35225771 35226409 35225771 ID=MS.gene50761.t1.exon2;Parent=MS.gene50761.t1
chr4.4 CDS 35225771 35226409 35225771 ID=cds.MS.gene50761.t1;Parent=MS.gene50761.t1
chr4.4 exon 35226509 35227048 35226509 ID=MS.gene50761.t1.exon3;Parent=MS.gene50761.t1
chr4.4 CDS 35226509 35227048 35226509 ID=cds.MS.gene50761.t1;Parent=MS.gene50761.t1
chr4.4 exon 35229603 35229656 35229603 ID=MS.gene50761.t1.exon4;Parent=MS.gene50761.t1
chr4.4 CDS 35229603 35229656 35229603 ID=cds.MS.gene50761.t1;Parent=MS.gene50761.t1
chr4.4 exon 35230381 35230653 35230381 ID=MS.gene50761.t1.exon5;Parent=MS.gene50761.t1
chr4.4 CDS 35230381 35230653 35230381 ID=cds.MS.gene50761.t1;Parent=MS.gene50761.t1
chr4.4 exon 35231506 35231569 35231506 ID=MS.gene50761.t1.exon6;Parent=MS.gene50761.t1
chr4.4 CDS 35231506 35231569 35231506 ID=cds.MS.gene50761.t1;Parent=MS.gene50761.t1
chr4.4 exon 35231673 35231881 35231673 ID=MS.gene50761.t1.exon7;Parent=MS.gene50761.t1
chr4.4 CDS 35231673 35231881 35231673 ID=cds.MS.gene50761.t1;Parent=MS.gene50761.t1
chr4.4 exon 35232423 35232575 35232423 ID=MS.gene50761.t1.exon8;Parent=MS.gene50761.t1
chr4.4 CDS 35232423 35232575 35232423 ID=cds.MS.gene50761.t1;Parent=MS.gene50761.t1
chr4.4 exon 35232969 35233073 35232969 ID=MS.gene50761.t1.exon9;Parent=MS.gene50761.t1
chr4.4 CDS 35232969 35233073 35232969 ID=cds.MS.gene50761.t1;Parent=MS.gene50761.t1
Gene Sequence

>MS.gene50761

ATGCTTGCTGATGGTTTTCATCTTGGTGGGAATACTCGGAGCGAAATACCAAAGAATTCTTCATCAGTTATCTCAAACATTCCGCGAAAGAGATGTTTTGATGAGTTAAGCTATCTCAACAACGGACAACCGCGCCAAAATCCAAGAGCTCTAATTGATGCCTATGGTAGTGACAAAAGCCAAGAAACTTCTGGCAGTAAGCCTTTGTTAGTGGAGCGACCGGATAAAAACAGTATGGATAAGTTTATGACAACATCATGGCAGAATACTGAAGAGGAGGAGTTCGATTGGGAAGATATGAGTCCGACACTAGTAGACCATAGTAGAAGTAATGGTTTCTTGCAACCAACTATTGGTTTCTCATCAGAAAAGCCTGTTACCGTTGCAGCAAGTGCAACTACTTCAGCGTCAAGGGTGTTTCCTGGTCTCAATTCTAATATTGAGTACCGTCCACCAGTTCTGCCAGCAACTTTTGAAACGAGGCGCTCCATAAATGTGCATGCTCCGCGTCCTACCAGCATAACTCCTATATTTCCATCGAAGAATCCTGTTAGGAATCCATTCGAATCAATAAATGCTAATAGTACCAGTGTGAGTAATGGTCTAATTAACAGACCTTTTCCTGTGCATGAACATGGTGTTGAGAACAACAATACCAGTAAAAGAAACCTGTATCAGTTGCCTAATCAGCTACCCGGTCTGATTTCATCTAACCCGCATAACAGTGGACAAACGCCACCATTCCGGTTTTTTCCATCTCAGGATCCAGCAGCGTCACAATTTACTTATAGCACTTCTTTGCCAGGACATTGTGCTGCTATGAGTAACCCCCTGCCAAATGCACAACTTGTTATGCCACTTCCTTCGCCAGGCCAAAGAATTGCAAACAGCTCTTTCAGTTTCCTGGGGGGAGCTTTGCCACCTTTACCTCCAGCTGGCCCACATGCTCTACCCCAGATTCTACCTAATCCCAATCCTGGTCCTTCTGTGTCAAGCCAACAGCCAACTGTTGGATATTCTAATTTGATTAGTTCACTCATGGCCCAAGGTGTTATGTCATTGGCTAATCAAGCCCCTGCGCTGGTAACTAAATCTATCCTTAGTTTAATTTTTATATCCTATTTTCCTTCCATTTTTGTTTGATTGCGTACCAATTTTGCAATTATATTATTATGTTTGTAGGATTTTGTTGGCATTGAGTTTGATCCAAATCTTCTGAAGGTTCGTCATGAATCTGCAATCAGTGCCTTATATGGCGATCTTCCTAGACAGTGCACAACTTGTGGCCTCCGATTCAAGTCCCAAGATGAGCACAGAAGTCACATGGATTGGCATGTGACTAAGAACCGTATGTCTAAAATCCATAAGCAGAAGCCCTCTCGCAAGTGGTTTGTGAGTGAGACGATGTGGCTAGGTGCAGAGGCATTGGGAGCTGAATCAGCTCCAGGTTTTTTGCCAACCGAGACCACTGAGGAAAAGAAAGAAGATGAAGAGTTGGCAGTTCTTGCTGACGAGGACCAAAATACATGTGCATTATGTAGAGAACCTTTTGACAAGTTTTACAGTGATGAAACAGAGGATTGGATGTATAGAGGAGCTGTATACCTTAATACGCCTAATGGAATAACAACAGGCATGGACAGGTCGCAGTTAGGCCCCATTATTCATGCTAAATGCATATCTGAATCTACCCCGTCTGAATATTTTGTGATGGATGAAGGGGTATGTGTTTCTTGTATTATTACTTTTCTATGCATTGTCTTGTTTTTGTTCCACTTTATGTCTTTAATTCATTGACTGACACATGTTTTTGGGGGAGAACAATACTAGTTGACTAGATTTCTTTGCAACTTTATGTTTTTTCTTTCCCATTGGTAAAACTTGTTTTTGAGGGAGATGTCCAGAATTCAGCAGCCATATTGTTCTTTTGGTGACTTCTCTTCAAGTGAGAATCAATCCATTATATCGTAAATCGCATGTTTTGGCTGAAATCCAAATGCAATGTCCTGTTTTTTCCGTCTTCTATGTCATGTCTTGCAAATGAGGATATCATGGTTTAAAATAGCTTGCAACACTAGGCATGGTAAAAACTGCAAATATATGTGCCTATTTAATGGCTGCCAACTTCACATGATGTTATTATTTCAAGTGGACTTTTGCATGTGCTGATGTTCTTCAAGCAGAGCAAATATTTATTCTTTTGTTTTCCTTGTATGCAGGGCACCTATGAAGAAGGCAGCCAAAGAAAACGAATGCAGAGCTGACTAAAGTAGTCCATTTATGTTCGCCGTAAGCCTGTAACTTACTAGTTTCGGATCAATGTATGCTCAATGTATGCAGGGCCTCTTGCCTAAATTATGTACATACATGTATAGATGCTCCTGTGTAACATGCATATCTTATTGCAGTCAAAAGTTTGCATTTAACCTGCAAATTTTCAGTACCTTACATTTATTGTCATTTCTTTTACTTCATTTTTGGCAGGAATTTGGCAAAGGATCCATGAGCACTTACTGTTTGAATTGTTATATCCAATTTAAATTGTGCATTGATGAATGAATGAGAATACTACAGATATTTGATGTTTTTTTTCCTTCTATAATTATTTCTGCTAGACATTTTATTTTAATGTCATTGGCTCTTTCTATAAGTTATTTTATTACTTTTTTCTCTATTATTTTGTGGTTCCTATGAATGAAAGTTTTTTTATAAGGTAAGAGATGGAGTCACCAACTATTTGTCTCTTTGTCCTTAAAAAAGAGATATCATTAATTCGTTTTACTATATTAGTATCTACAATCTAAATCAAAAGCTTAATTAACTATATAAAAAAAATTAGTATCTACAATATTGACTAAACAAGACTTATTAGTTCGAAATATAGGGCGCTATCTTTCACACGAGCCAAATTATCGCAAAGATCACTAAAGAATGTAAATTAGTTAATTAATCAAACAAGTGATTATACATAAACATGATAACTTATCCTATCAAACTCCTCTTATCCAGACAGTCAAACAAAGTTATAGGTTGTATTGTATTTGAATCCACACTTTTTGTTCATGAAAACATAGTGACAAACACCACAAGAAATTCACATTTCAAGGTTCCAAGTTCGGATCCAAATAAGATGTCTCCCATACCGATATTGATATTGCCAGTCAAATTAGGTCTCATGAACTGAATCCAAACCTTTTTTCTGTGTGTTGTCTCATTCCTTTGAACTCTAAAACGTTGCTTCTCCCTCACTATGCAGTGTTACTATGAAACACTGAATCTTCAAACACATTTCATCCACAATTGTAACGATTAACTCAAATTTCTTCGAAGTGTCATTTTTTATTATTTTATTTTCTCATGAGGTGCTATTGGGAAATATTTGGATTTCTACACACAATTGAATCAATTAACGCCAAACTTGGTTAAGGTGAAAGAATCGTGTTTGGTTCGCATAATGTTATAAATAAATTAGTAGTAACATTTTTTTTTTTTTTACAAATTATTAGTAGTAACAATGACTTGTAAGCAAAATTCCTAAAAAAAAAAAAAGATTTATAAGCAAAAGATAGCTCAATTATCTTAAAAAGAAATATTGTAAAAAAAAGTGCTCGAACATGATCACTTTTTAAAGAGAGAAAGACTTGACAAAAAGGTTACAAGATCTAAGGTTTGTGTTTGTGATCTTAAAGACACTCAAATATTGTGATACTTAATACTTGTTTGAGATGAAACGATAAAACTAAACACACTCAAATATTGTGATCTACGAATTTGAAAAAAGTGCTGAAATGCTTGATATAAAGAGCCTTAGGCTTGGGAGTTTGGAGGGGAGGGTAGGTGAGAGTTTTGGAAAAAAGGAAGGAGCAAGTGGAAGAAATAGACGACATTGAAGGAGAGGGCTTTGGAGGTTAATTTTTTACTCATAATAAAAAATCCCCCATCATTTGGGGGAACTCAAAAATTGTATTGGGGGAGGGTTTGGGGGGGGGGGGGGGGGTTAGGGAGGGTTTATATGAATTTTTCAAATTTAATTATATTCTTAATATTTAAAGTAGAGAGAGAGGGAAAAGAAAATCAAGCAAGTATACTGCGAGAAAAGTGAAACTTGGGACGAAGCGAGTCCCTCGAAGAGTTAGCCTGATTACTGTAGCGCTTCATTTTTGTTTCCTTCATTCAGTTTCTGCAATTTCTGGGTTAACTCCCAGTTTTCATCAATATTAGAGTGGGTTCTATCAATTTCCAATTTTTTAACAAATTAAAATTGTATTTCACTCAGCTTTTGTTTTCAGTATTTCATATCAGGAGGTGGCAGGAAAATCCCCTTGCACAGGTAAAATAAATCACTAGTACTTGTTGCTGCATTTTTGGCAGCACTTTAATTTTTGAATACCAAAATCATACTTTACTTGGGTCTGGGTCACTGTTATGATGCCATAGTCTGATTTATCTATTTAAAGATAGTTGGCATGTTAAGTGAAAATAAAGTTTATTTAAGATAGTTAGGTTATGTACTGGTCGTCTATTGATGCATTATTTAATTAATGTTTCAATTCTGGATGCTGATATAGAAAATACTTACTCGACTACTTCTCTCATACTGCTTTACTGTCCTTAACTTGAGCTTGATAATACTGTTTGAATGGTTGTTAGATGGATTTACAACCGTTCTTTAAGCACATATATCATTTGAGATCTCATTCTGCATATTTGTCTTTGCAACTGAGGCAGCTGCAAGTAAAGTTATTGTGCTCACTGAGGCAGCTGCAATTAAGGGAAGAAGACTCGTCATGGAAAGATTTTCTCTCTTCTGGAAGTCAGAGCTCCAGTTATTGCATATTGCAATGAATTCATTAGACTATACCTTAATTTAATTAAGTGGTCCCTTGTACATACTAAATACTCACAGCAAAATATCCCGAAAATGGGGTCAAATGAGACAAATTGAGAAATTTCATGTTTTCTCTTTCGTAAGTTTTGTTTAATCTGACCTTAATATACTATTCTCATCTGGAACCACAGATAAAGATTATCCCTTATTATGTATGACATATCAGGTAGAGACCACTCATTATAATGATTTTCTGGTTATATTTTGGCTTTTATCAGGAGATCCAAAAGCTATTCCTTGGACTCGACTTGCCCCAATACGAAGTGCTCTTGATGGATGGGCTGCTATTGACATTCAAGTTGGGGATGTCTATTGCTGGCCTACAAATTTGGGATGGGTGATAGGACCTACTCTGTTATATCATTGCTTTCTAAATGGTGCATCTCTTTCTTTGTACCATGGATCTCCTCAAGGCCATGGTTTTGGGAAATTTGTTCAGGTGAGTCTCCAATTTAATATTTCATTACTTTTATAATTTTGCCATACATAGATGATTAATTTATTTAGTATTTGCTTCTGCATATGATGGTTATCAATTATTGTTTTGATCAACTAAAATGTTGGAACAACAAACAAAAATATAAACGCTGATTTGTAATGAGAACCATTACTACAGTTCTCCTCACGATTACTGATTTGTAAAATAATGGAGGAAATAATGGGAATGAGAAACGGAGAGGATCCACCGCAGAGTATAAAGACCACCTCACCACGAAAATCTGTTTCATTCCTATAGTTGACCATTCTCGATGGAATAAGGTTGGCGTCCGACTCTATATCATCATCACCTCAAATCCACTATTCATTTACTATCATCAAACTTTGACCCAAAAAGCTGGTTAAAGAACTAATGTTTGGGGATGTTACATAGATGAAACACGTTTATGATAATCAGTCTCCCCTGCATATCTCTCAAATCCAATATTCCACAAGGGAACCAAACATTTGAGATAGAATGTCATTTCATTGAAGGAAGGATCTTACCTAAAGACATTATCACTAGCTTCATAAATTGGAAGATGTGTTCCCTTTAAATTAGTTATATATGTAACAAATATGATTTATAATCTTCAGTTGGAGGGGGAGTGTTCGAAATAGTTTAAATAGGGTAATGATTCTAAGTAGTAACAGTAACCGTACATCGTTGTGTCTATACAGCATTCTCAGATTTACTCATTCTCAGATTTATCTCAGATTCACAGATTCTATAACATGCCTATCATCTATTCTCCTTCTCAGCAAATACATCACACCAAGGCACAACTTTACTCATTGTACTATGATTGTTCAGGATGCAGGTGTTACCATTTTGGGAACCGTTCCAAGCTTAGTGAAATCTTGGAAGAGTACTCGATGTATGGTGGGCTTGGATTGGACAAAAATAAAGTAATATCTATTGAAAGTGTAAAGAATAAAATAGCTACTAAGAAAGATGAACTAAACTGCCCTTGTATGCAGGTTATTTTGGAGAAACGTCAAATATTGATGATGATCTGTGGCTTTCTTCAAAGTCATATTACAATCCAATCATTGAATTATGTGGAGGAACTGAGCTTGCGTCCAGTTACATTACGGGAAGTCCCCTGCGGCCTCAAGCTTTTGGAGCATTCAGCACAGCATCAATGACAACTGGCTTTGTCATTCTTGACGACAATGGAGTTCCTTATGTAAGAGCTTTTTCTGGATAAGTTATATTCTTTGGTCATGATTTTATTTACTTGTGCTGCTAGTCTGCCACTTCTCACCACATTACATTGTGCCAATACATCAGTATAGTTTTTTTGTGGCAAAGGTGATGGATGTGTGCTTACTTCATCTTCCAGACTAGGGAATGGAAAGGTTATTATGAACTATGAAGCACTGATAACACACAAACACTGGACATTGATAACAATTTTAAAAATTGAATTAATTGAATGTTATCACATGTGTACGCCTCTGATCAGAAGTGTCAGTGCTACAGAGTATGTTGGCGTGATAATGGCCAATAATACACCCTAGTCTCAAAATCTATGATCCATAGTTCCTAATGGCCATCAATTTGAATTCTGCACATGAATCTTTTTTCAATACTTGTTTCTGTACAGGTCTAGTTCTTATGCTAGCCCTATGTTCACCACCTTTTTCTTGTAACTTTCAGTCCCTATTGCACTCTTAAATTTATTGATAGGTTTTCTGATTTATTGCTCTCTTTGACCAATATGCCAGCTAGAAAATGTTCCTTTTTTGAAGAAACTAGAAAATGTTCCTTGTGTTGGTGAAGTGGGTTTATTCCCTGTCTCTCTTGGAGCATCTGATAGATTGCTGAATGCCGACCATGATGAGGTTTACTTTAAGGGAATGCCCTTGCATAAAGGAAAGGTATCTACCACTTCAATTAATATGATTAGAATGATTTCCCCATTTTGTCTCGACATACCAACACATTTCGATACAACTACTTTGAAATTTCAGTTCATTCATTCTCGCTATGCGTCCTAAAGGCTTTTAAGAATTTTTTTTTCATGTGCAATTACCAAATTACACATCGAACATCGAAATTAGATCGAGCAAAAAGATAGGAAAATCTTTTATTCTGAACTTTTGGATAAATCAAATGTCCAAAAGAACCGTATATTAGTTAGATATACATTAAGGATTTTCAAACAATCCCTTCATTATAAATTACATAGGCTTAATTTGTAATTTTGACTAGTCTCATTCTGCTATTATTGCTATAACTTGTCATGTCTTTCATGATATGTGTATTAGCAGGTTCTTAGGAGACATGGAGATATAATCAAGAGAATGGTTGGTGGCTATATCATCGTACAAGGGAGGGCTGATGACACCATGAACATTGGTGGAATAAATGCGTAA
Protein sequence

>MS.gene50761.t1

MLADGFHLGGNTRSEIPKNSSSVISNIPRKRCFDELSYLNNGQPRQNPRALIDAYGSDKSQETSGSKPLLVERPDKNSMDKFMTTSWQNTEEEEFDWEDMSPTLVDHSRSNGFLQPTIGFSSEKPVTVAASATTSASRYRPPVLPATFETRRSINVHAPRPTSITPIFPSKNPVRNPFESINANSTSVSNGLINRPFPVHEHGVENNNTSKRNLYQLPNQLPGLISSNPHNSGQTPPFRFFPSQDPAASQFTYSTSLPGHCAAMSNPLPNAQLVMPLPSPGQRIANSSFSFLGGALPPLPPAGPHALPQILPNPNPGPSVSSQQPTVGYSNLISSLMAQGVMSLANQAPALDFVGIEFDPNLLKVRHESAISALYGDLPRQCTTCGLRFKSQDEHRSHMDWHVTKNRMSKIHKQKPSRKWFVSETMWLGAEALGAESAPGFLPTETTEEKKEDEELAVLADEDQNTCALCREPFDKFYSDETEDWMYRGAVYLNTPNGITTGMDRSQLGPIIHAKCISESTPSEYFVMDEGLLFSVFHIRRWQENPLAQVETTHYNDFLVIFWLLSGDPKAIPWTRLAPIRSALDGWAAIDIQVGDVYCWPTNLGWVIGPTLLYHCFLNGASLSLYHGSPQGHGFGKFVQDAGVTILGTVPSLVKSWKSTRCYFGETSNIDDDLWLSSKSYYNPIIELCGGTELASSYITGSPLRPQAFGAFSTASMTTGFVILDDNGVPYLENVPFLKKLENVPCVGEVGLFPVSLGASDRLLNADHDEVYFKGMPLHKGKVLRRHGDIIKRMVGGYIIVQGRADDTMNIGGINA