Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50769.t1 | RHN79076.1 | 68 | 266 | 67 | 4 | 1 | 252 | 1 | 262 | 4.00E-64 | 255 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50769.t1 | A0A396JLE5 | 68.0 | 266 | 67 | 4 | 1 | 252 | 1 | 262 | 2.9e-64 | 255.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene50769.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50769.t1 | MTR_4g073310 | 69.176 | 279 | 31 | 3 | 1 | 276 | 1 | 227 | 1.74e-114 | 328 |
| MS.gene50769.t1 | MTR_1g052185 | 42.025 | 326 | 84 | 11 | 1 | 272 | 1 | 275 | 1.18e-55 | 181 |
| MS.gene50769.t1 | MTR_1g046810 | 34.967 | 306 | 131 | 12 | 20 | 276 | 28 | 314 | 1.00e-33 | 125 |
| MS.gene50769.t1 | MTR_1g053530 | 34.826 | 201 | 104 | 7 | 23 | 222 | 25 | 199 | 3.45e-21 | 90.1 |
| MS.gene50769.t1 | MTR_6g011180 | 77.778 | 36 | 8 | 0 | 167 | 202 | 200 | 235 | 5.60e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50769.t1 | AT2G45120 | 28.682 | 129 | 80 | 1 | 19 | 135 | 180 | 308 | 8.30e-11 | 62.0 |
Find 51 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGATCATGAACATCTTAA+TGG | 0.235891 | 4.1:+30437302 | MS.gene50769:CDS |
| TTTCTCATTATCGTCCTTAA+TGG | 0.255039 | 4.1:-30437048 | None:intergenic |
| AGAAGAACATGGATTATAAA+AGG | 0.308077 | 4.1:-30436898 | None:intergenic |
| AAATTCATAACAACTCCTTT+TGG | 0.331715 | 4.1:-30437635 | None:intergenic |
| CCGCCGCTCTCATACCATTA+AGG | 0.353355 | 4.1:+30437034 | MS.gene50769:CDS |
| CCTCCTGATAGAACGTTTGT+TGG | 0.361348 | 4.1:-30437270 | None:intergenic |
| TGCGCTGCGGCTGGTGGTGG+TGG | 0.387987 | 4.1:+30437350 | MS.gene50769:CDS |
| AAACCAACAAACGTTCTATC+AGG | 0.407703 | 4.1:+30437267 | MS.gene50769:CDS |
| GCTGGTGGTGGTGGTGATCA+TGG | 0.417182 | 4.1:+30437359 | MS.gene50769:CDS |
| GGACTTTGGTGGCAAATACT+TGG | 0.426874 | 4.1:-30437240 | None:intergenic |
| AACTCACACTCATACATTCT+CGG | 0.428608 | 4.1:-30436957 | None:intergenic |
| ATGTCCGCGAAAAGCCTTGT+TGG | 0.435044 | 4.1:-30437111 | None:intergenic |
| AATAGTGAAGGCAATGGGAC+TGG | 0.441225 | 4.1:+30437584 | MS.gene50769:CDS |
| TCTTGCGCTGCGGCTGGTGG+TGG | 0.459861 | 4.1:+30437347 | MS.gene50769:CDS |
| GAGTTTGCGGCTGATATTCA+AGG | 0.475940 | 4.1:+30437511 | MS.gene50769:CDS |
| TATGATATTGCACCGTGAAA+AGG | 0.476814 | 4.1:+30437133 | MS.gene50769:CDS |
| TCTAATGAATTGAAGCCAAA+AGG | 0.489056 | 4.1:+30437620 | MS.gene50769:CDS |
| TCATGTGCAACATCTTCTGT+TGG | 0.490922 | 4.1:-30437674 | None:intergenic |
| TTGAAATTGTTCCAAGGATG+TGG | 0.492696 | 4.1:-30437180 | None:intergenic |
| AATATGTCTTGCGCTGCGGC+TGG | 0.494836 | 4.1:+30437341 | MS.gene50769:CDS |
| TCAATCGCCAACCACATCCT+TGG | 0.495376 | 4.1:+30437169 | MS.gene50769:CDS |
| GGTTGGCGATTGAATACTCT+TGG | 0.497146 | 4.1:-30437159 | None:intergenic |
| GAACAATTTCAATTTCAAGT+TGG | 0.498276 | 4.1:+30437191 | MS.gene50769:CDS |
| AAATACTTGGTTAAGTCAAT+CGG | 0.503127 | 4.1:-30437227 | None:intergenic |
| TTCAACTCTGGCAACGCACT+CGG | 0.512404 | 4.1:+30436993 | MS.gene50769:CDS |
| TTGAAATTGAAATTGTTCCA+AGG | 0.519609 | 4.1:-30437186 | None:intergenic |
| CTTGCACTCATGAGGAAGAA+AGG | 0.532255 | 4.1:+30436861 | None:intergenic |
| ATTATTATTGACCTTCACCT+TGG | 0.544902 | 4.1:-30437471 | None:intergenic |
| ATGTCTTGCGCTGCGGCTGG+TGG | 0.545212 | 4.1:+30437344 | MS.gene50769:CDS |
| GACAAGATCGAGAAACAAGT+TGG | 0.568686 | 4.1:+30437425 | MS.gene50769:CDS |
| ATAGTGAAGGCAATGGGACT+GGG | 0.570844 | 4.1:+30437585 | MS.gene50769:CDS |
| TTGGTTAAGTCAATCGGTGG+TGG | 0.571109 | 4.1:-30437221 | None:intergenic |
| TGGTGACATTGATGAAACTT+CGG | 0.577161 | 4.1:+30437445 | MS.gene50769:CDS |
| TGATGAAACTTCGGCGACCA+AGG | 0.579505 | 4.1:+30437454 | MS.gene50769:CDS |
| AGTGTGAGTTGTGCGGCAAA+AGG | 0.579769 | 4.1:+30436970 | MS.gene50769:CDS |
| TGCGGCAAAAGGTTCAACTC+TGG | 0.586813 | 4.1:+30436981 | MS.gene50769:CDS |
| TAGTTGTCCTGTTTGCAACA+AGG | 0.599547 | 4.1:+30437079 | MS.gene50769:CDS |
| TGAAGGCAATGGGACTGGGA+TGG | 0.605766 | 4.1:+30437589 | MS.gene50769:CDS |
| AACTTCGGCGACCAAGGTGA+AGG | 0.611419 | 4.1:+30437460 | MS.gene50769:CDS |
| AACTCTGGCAACGCACTCGG+TGG | 0.616672 | 4.1:+30436996 | MS.gene50769:CDS |
| CTTCAATATGTCTTGCGCTG+CGG | 0.618324 | 4.1:+30437337 | MS.gene50769:CDS |
| AATTGTTCCAAGGATGTGGT+TGG | 0.627602 | 4.1:-30437176 | None:intergenic |
| GGATTATAAAAGGAACAAGA+AGG | 0.636811 | 4.1:-30436888 | None:intergenic |
| AATGAATGAAAAGAAGAACA+TGG | 0.641497 | 4.1:-30436909 | None:intergenic |
| TACTTGGTTAAGTCAATCGG+TGG | 0.654609 | 4.1:-30437224 | None:intergenic |
| CCAACAAACGTTCTATCAGG+AGG | 0.665230 | 4.1:+30437270 | MS.gene50769:CDS |
| GCTCAAAGTTTCAAGCAATG+TGG | 0.669423 | 4.1:+30437397 | MS.gene50769:CDS |
| ATTCAAGGTTAATGACAACA+AGG | 0.683610 | 4.1:+30437526 | MS.gene50769:CDS |
| CCTTAATGGTATGAGAGCGG+CGG | 0.689827 | 4.1:-30437034 | None:intergenic |
| CGTCCTTAATGGTATGAGAG+CGG | 0.696710 | 4.1:-30437037 | None:intergenic |
| ATGTATGAGTGTGAGTTGTG+CGG | 0.703631 | 4.1:+30436963 | MS.gene50769:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATACTTGGTTAAGTCAAT+CGG | - | chr4.1:30437230-30437249 | None:intergenic | 25.0% |
| ! | AATGAATGAAAAGAAGAACA+TGG | - | chr4.1:30436912-30436931 | None:intergenic | 25.0% |
| ! | ATAACAGTAAAAAGAGTTTG+CGG | + | chr4.1:30437498-30437517 | MS.gene50769:CDS | 25.0% |
| ! | ATTGATCATGAACATCTTAA+TGG | + | chr4.1:30437302-30437321 | MS.gene50769:CDS | 25.0% |
| ! | GAAAACAAGAAAAATAGTGA+AGG | + | chr4.1:30437572-30437591 | MS.gene50769:CDS | 25.0% |
| ! | TTGAAATTGAAATTGTTCCA+AGG | - | chr4.1:30437189-30437208 | None:intergenic | 25.0% |
| !! | AAATTCATAACAACTCCTTT+TGG | - | chr4.1:30437638-30437657 | None:intergenic | 25.0% |
| !! | AGAAGAACATGGATTATAAA+AGG | - | chr4.1:30436901-30436920 | None:intergenic | 25.0% |
| !! | GAACAATTTCAATTTCAAGT+TGG | + | chr4.1:30437191-30437210 | MS.gene50769:CDS | 25.0% |
| !!! | TTTTCTTGTTTTCTTCATCT+TGG | - | chr4.1:30437565-30437584 | None:intergenic | 25.0% |
| AAGAAAAATAGTGAAGGCAA+TGG | + | chr4.1:30437578-30437597 | MS.gene50769:CDS | 30.0% | |
| AGAAAAATAGTGAAGGCAAT+GGG | + | chr4.1:30437579-30437598 | MS.gene50769:CDS | 30.0% | |
| ATTATTATTGACCTTCACCT+TGG | - | chr4.1:30437474-30437493 | None:intergenic | 30.0% | |
| ATTCAAGGTTAATGACAACA+AGG | + | chr4.1:30437526-30437545 | MS.gene50769:CDS | 30.0% | |
| GGATTATAAAAGGAACAAGA+AGG | - | chr4.1:30436891-30436910 | None:intergenic | 30.0% | |
| TCTAATGAATTGAAGCCAAA+AGG | + | chr4.1:30437620-30437639 | MS.gene50769:CDS | 30.0% | |
| TTTCTCATTATCGTCCTTAA+TGG | - | chr4.1:30437051-30437070 | None:intergenic | 30.0% | |
| !!! | AGAACGTTTGTTGGTTTTAT+GGG | - | chr4.1:30437264-30437283 | None:intergenic | 30.0% |
| !!! | TAGAACGTTTGTTGGTTTTA+TGG | - | chr4.1:30437265-30437284 | None:intergenic | 30.0% |
| AAACCAACAAACGTTCTATC+AGG | + | chr4.1:30437267-30437286 | MS.gene50769:CDS | 35.0% | |
| AACTCACACTCATACATTCT+CGG | - | chr4.1:30436960-30436979 | None:intergenic | 35.0% | |
| GAAAAAACCTTGTTGCAAAC+AGG | - | chr4.1:30437089-30437108 | None:intergenic | 35.0% | |
| TATGATATTGCACCGTGAAA+AGG | + | chr4.1:30437133-30437152 | MS.gene50769:CDS | 35.0% | |
| TGGTGACATTGATGAAACTT+CGG | + | chr4.1:30437445-30437464 | MS.gene50769:CDS | 35.0% | |
| TTGAAATTGTTCCAAGGATG+TGG | - | chr4.1:30437183-30437202 | None:intergenic | 35.0% | |
| ! | TACTCTTGGAATCCTTTTCA+CGG | - | chr4.1:30437148-30437167 | None:intergenic | 35.0% |
| !!! | CAAGGTTTTTTCATCCAACA+AGG | + | chr4.1:30437097-30437116 | MS.gene50769:CDS | 35.0% |
| !!! | TTGTTGGTTTTATGGGACTT+TGG | - | chr4.1:30437257-30437276 | None:intergenic | 35.0% |
| AATTGTTCCAAGGATGTGGT+TGG | - | chr4.1:30437179-30437198 | None:intergenic | 40.0% | |
| ATGTATGAGTGTGAGTTGTG+CGG | + | chr4.1:30436963-30436982 | MS.gene50769:CDS | 40.0% | |
| GACAAGATCGAGAAACAAGT+TGG | + | chr4.1:30437425-30437444 | MS.gene50769:CDS | 40.0% | |
| GCTCAAAGTTTCAAGCAATG+TGG | + | chr4.1:30437397-30437416 | MS.gene50769:CDS | 40.0% | |
| TACTTGGTTAAGTCAATCGG+TGG | - | chr4.1:30437227-30437246 | None:intergenic | 40.0% | |
| TAGTTGTCCTGTTTGCAACA+AGG | + | chr4.1:30437079-30437098 | MS.gene50769:CDS | 40.0% | |
| TCATGTGCAACATCTTCTGT+TGG | - | chr4.1:30437677-30437696 | None:intergenic | 40.0% | |
| !!! | TTGGTTTTATGGGACTTTGG+TGG | - | chr4.1:30437254-30437273 | None:intergenic | 40.0% |
| AATAGTGAAGGCAATGGGAC+TGG | + | chr4.1:30437584-30437603 | MS.gene50769:CDS | 45.0% | |
| ATAGTGAAGGCAATGGGACT+GGG | + | chr4.1:30437585-30437604 | MS.gene50769:CDS | 45.0% | |
| CCAACAAACGTTCTATCAGG+AGG | + | chr4.1:30437270-30437289 | MS.gene50769:CDS | 45.0% | |
| CCTCCTGATAGAACGTTTGT+TGG | - | chr4.1:30437273-30437292 | None:intergenic | 45.0% | |
| CGTCCTTAATGGTATGAGAG+CGG | - | chr4.1:30437040-30437059 | None:intergenic | 45.0% | |
| CTTCAATATGTCTTGCGCTG+CGG | + | chr4.1:30437337-30437356 | MS.gene50769:CDS | 45.0% | |
| GAGTTTGCGGCTGATATTCA+AGG | + | chr4.1:30437511-30437530 | MS.gene50769:CDS | 45.0% | |
| GGTTGGCGATTGAATACTCT+TGG | - | chr4.1:30437162-30437181 | None:intergenic | 45.0% | |
| TTGGTTAAGTCAATCGGTGG+TGG | - | chr4.1:30437224-30437243 | None:intergenic | 45.0% | |
| ! | GGACTTTGGTGGCAAATACT+TGG | - | chr4.1:30437243-30437262 | None:intergenic | 45.0% |
| ! | TCATCCAACAAGGCTTTTCG+CGG | + | chr4.1:30437107-30437126 | MS.gene50769:CDS | 45.0% |
| AGTGTGAGTTGTGCGGCAAA+AGG | + | chr4.1:30436970-30436989 | MS.gene50769:CDS | 50.0% | |
| CCTTAATGGTATGAGAGCGG+CGG | - | chr4.1:30437037-30437056 | None:intergenic | 50.0% | |
| TCAATCGCCAACCACATCCT+TGG | + | chr4.1:30437169-30437188 | MS.gene50769:CDS | 50.0% | |
| TGATGAAACTTCGGCGACCA+AGG | + | chr4.1:30437454-30437473 | MS.gene50769:CDS | 50.0% | |
| TGCGGCAAAAGGTTCAACTC+TGG | + | chr4.1:30436981-30437000 | MS.gene50769:CDS | 50.0% | |
| TTCAACTCTGGCAACGCACT+CGG | + | chr4.1:30436993-30437012 | MS.gene50769:CDS | 50.0% | |
| ! | ATGTCCGCGAAAAGCCTTGT+TGG | - | chr4.1:30437114-30437133 | None:intergenic | 50.0% |
| AACTTCGGCGACCAAGGTGA+AGG | + | chr4.1:30437460-30437479 | MS.gene50769:CDS | 55.0% | |
| AATATGTCTTGCGCTGCGGC+TGG | + | chr4.1:30437341-30437360 | MS.gene50769:CDS | 55.0% | |
| CCGCCGCTCTCATACCATTA+AGG | + | chr4.1:30437034-30437053 | MS.gene50769:CDS | 55.0% | |
| TGAAGGCAATGGGACTGGGA+TGG | + | chr4.1:30437589-30437608 | MS.gene50769:CDS | 55.0% | |
| ! | AGCGGCGGTGAGATGTTTTG+TGG | - | chr4.1:30437022-30437041 | None:intergenic | 55.0% |
| AACTCTGGCAACGCACTCGG+TGG | + | chr4.1:30436996-30437015 | MS.gene50769:CDS | 60.0% | |
| !! | GCTGGTGGTGGTGGTGATCA+TGG | + | chr4.1:30437359-30437378 | MS.gene50769:CDS | 60.0% |
| ATGTCTTGCGCTGCGGCTGG+TGG | + | chr4.1:30437344-30437363 | MS.gene50769:CDS | 65.0% | |
| TCTTGCGCTGCGGCTGGTGG+TGG | + | chr4.1:30437347-30437366 | MS.gene50769:CDS | 70.0% | |
| !! | TGCGCTGCGGCTGGTGGTGG+TGG | + | chr4.1:30437350-30437369 | MS.gene50769:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 30436870 | 30437700 | 30436870 | ID=MS.gene50769 |
| chr4.1 | mRNA | 30436870 | 30437700 | 30436870 | ID=MS.gene50769.t1;Parent=MS.gene50769 |
| chr4.1 | exon | 30436870 | 30437700 | 30436870 | ID=MS.gene50769.t1.exon1;Parent=MS.gene50769.t1 |
| chr4.1 | CDS | 30436870 | 30437700 | 30436870 | ID=cds.MS.gene50769.t1;Parent=MS.gene50769.t1 |
| Gene Sequence |
| Protein sequence |
