Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50842.t1 | XP_003598025.2 | 81.1 | 387 | 72 | 1 | 98 | 483 | 156 | 542 | 2.90E-174 | 621.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50842.t1 | Q8L3W1 | 29.4 | 340 | 149 | 8 | 230 | 482 | 6 | 341 | 1.6e-27 | 125.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50842.t1 | G7IYE4 | 81.1 | 387 | 72 | 1 | 98 | 483 | 156 | 542 | 2.1e-174 | 621.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene50842.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50842.t1 | MTR_3g005420 | 81.137 | 387 | 72 | 1 | 98 | 483 | 156 | 542 | 0.0 | 626 |
| MS.gene50842.t1 | MTR_3g005420 | 52.158 | 278 | 107 | 4 | 199 | 474 | 11 | 264 | 2.57e-83 | 267 |
| MS.gene50842.t1 | MTR_3g005420 | 73.127 | 387 | 68 | 2 | 98 | 483 | 137 | 488 | 0.0 | 544 |
| MS.gene50842.t1 | MTR_3g005420 | 54.307 | 267 | 96 | 4 | 210 | 474 | 3 | 245 | 2.40e-83 | 266 |
| MS.gene50842.t1 | MTR_3g005460 | 67.237 | 409 | 116 | 3 | 83 | 483 | 125 | 523 | 0.0 | 531 |
| MS.gene50842.t1 | MTR_3g005460 | 55.466 | 247 | 87 | 2 | 225 | 471 | 20 | 243 | 3.66e-83 | 266 |
| MS.gene50842.t1 | MTR_3g005495 | 55.032 | 467 | 163 | 11 | 22 | 475 | 41 | 473 | 2.94e-157 | 455 |
| MS.gene50842.t1 | MTR_3g005495 | 53.165 | 237 | 80 | 3 | 245 | 480 | 28 | 234 | 9.04e-75 | 243 |
| MS.gene50842.t1 | MTR_5g070440 | 68.248 | 274 | 85 | 1 | 210 | 483 | 3 | 274 | 1.36e-132 | 384 |
| MS.gene50842.t1 | MTR_5g070440 | 50.407 | 123 | 56 | 3 | 84 | 205 | 147 | 265 | 6.92e-25 | 104 |
| MS.gene50842.t1 | MTR_3g005440 | 59.944 | 357 | 107 | 4 | 83 | 437 | 120 | 442 | 3.02e-131 | 387 |
| MS.gene50842.t1 | MTR_3g005440 | 49.785 | 233 | 66 | 4 | 244 | 474 | 34 | 217 | 1.26e-59 | 202 |
| MS.gene50842.t1 | MTR_3g005490 | 69.615 | 260 | 68 | 2 | 225 | 483 | 16 | 265 | 3.41e-129 | 375 |
| MS.gene50842.t1 | MTR_3g005490 | 43.353 | 173 | 80 | 8 | 36 | 205 | 99 | 256 | 8.73e-23 | 98.2 |
| MS.gene50842.t1 | MTR_3g005480 | 67.954 | 259 | 71 | 2 | 225 | 481 | 16 | 264 | 7.37e-124 | 362 |
| MS.gene50842.t1 | MTR_3g005480 | 46.528 | 144 | 66 | 5 | 65 | 205 | 122 | 257 | 2.39e-21 | 94.0 |
| MS.gene50842.t1 | MTR_6g472230 | 61.702 | 94 | 36 | 0 | 1 | 94 | 117 | 210 | 4.50e-30 | 125 |
| MS.gene50842.t1 | MTR_1g015350 | 37.143 | 210 | 38 | 5 | 273 | 482 | 58 | 173 | 1.88e-28 | 111 |
| MS.gene50842.t1 | MTR_7g050560 | 28.276 | 290 | 162 | 6 | 229 | 480 | 12 | 293 | 2.04e-27 | 112 |
| MS.gene50842.t1 | MTR_3g061350 | 29.225 | 284 | 153 | 8 | 230 | 476 | 12 | 284 | 2.30e-26 | 108 |
| MS.gene50842.t1 | MTR_7g050710 | 27.832 | 309 | 170 | 10 | 223 | 480 | 4 | 310 | 4.22e-26 | 108 |
| MS.gene50842.t1 | MTR_8g103940 | 25.667 | 300 | 164 | 6 | 230 | 477 | 39 | 331 | 6.58e-26 | 110 |
| MS.gene50842.t1 | MTR_1g034240 | 43.636 | 110 | 62 | 0 | 230 | 339 | 7 | 116 | 1.33e-25 | 109 |
| MS.gene50842.t1 | MTR_1g034240 | 35.659 | 129 | 72 | 4 | 356 | 477 | 268 | 392 | 1.48e-14 | 75.9 |
| MS.gene50842.t1 | MTR_3g098790 | 27.491 | 291 | 172 | 7 | 229 | 480 | 12 | 302 | 2.34e-25 | 106 |
| MS.gene50842.t1 | MTR_5g044670 | 24.415 | 299 | 184 | 7 | 223 | 480 | 9 | 306 | 3.59e-25 | 106 |
| MS.gene50842.t1 | MTR_3g061310 | 28.417 | 278 | 164 | 5 | 230 | 476 | 12 | 285 | 5.91e-25 | 104 |
| MS.gene50842.t1 | MTR_3g061370 | 29.021 | 286 | 157 | 9 | 227 | 476 | 6 | 281 | 1.60e-24 | 103 |
| MS.gene50842.t1 | MTR_1g108780 | 27.083 | 288 | 148 | 10 | 245 | 478 | 12 | 291 | 2.82e-24 | 105 |
| MS.gene50842.t1 | MTR_7g050580 | 27.609 | 297 | 166 | 8 | 223 | 480 | 7 | 293 | 6.99e-24 | 102 |
| MS.gene50842.t1 | MTR_3g061340 | 29.151 | 271 | 160 | 8 | 230 | 476 | 12 | 274 | 3.51e-22 | 96.7 |
| MS.gene50842.t1 | MTR_4g068320 | 41.176 | 102 | 60 | 0 | 227 | 328 | 3 | 104 | 2.35e-21 | 96.7 |
| MS.gene50842.t1 | MTR_4g068320 | 30.526 | 190 | 102 | 6 | 313 | 480 | 210 | 391 | 9.09e-15 | 76.6 |
| MS.gene50842.t1 | MTR_3g061320 | 28.881 | 277 | 160 | 7 | 229 | 476 | 11 | 279 | 2.99e-21 | 94.0 |
| MS.gene50842.t1 | MTR_1g035460 | 26.531 | 294 | 146 | 8 | 246 | 477 | 2 | 287 | 8.57e-21 | 94.7 |
| MS.gene50842.t1 | MTR_1g034210 | 40.909 | 110 | 63 | 1 | 230 | 339 | 7 | 114 | 1.79e-20 | 92.4 |
| MS.gene50842.t1 | MTR_1g034210 | 40.909 | 110 | 63 | 1 | 230 | 339 | 7 | 114 | 3.54e-20 | 92.4 |
| MS.gene50842.t1 | MTR_1g034210 | 38.281 | 128 | 54 | 6 | 364 | 477 | 242 | 358 | 7.88e-14 | 73.2 |
| MS.gene50842.t1 | MTR_6g075400 | 50.667 | 75 | 37 | 0 | 16 | 90 | 117 | 191 | 1.15e-19 | 87.4 |
| MS.gene50842.t1 | MTR_7g050720 | 26.923 | 312 | 160 | 9 | 229 | 477 | 9 | 315 | 1.67e-19 | 89.7 |
| MS.gene50842.t1 | MTR_0152s0100 | 40.179 | 112 | 66 | 1 | 370 | 481 | 2 | 112 | 1.69e-19 | 84.3 |
| MS.gene50842.t1 | MTR_0152s0100 | 40.179 | 112 | 66 | 1 | 370 | 481 | 2 | 112 | 1.69e-19 | 84.3 |
| MS.gene50842.t1 | MTR_7g050590 | 27.397 | 292 | 145 | 9 | 246 | 480 | 2 | 283 | 2.02e-18 | 86.3 |
| MS.gene50842.t1 | MTR_3g061330 | 30.204 | 245 | 135 | 7 | 230 | 443 | 12 | 251 | 4.80e-18 | 84.0 |
| MS.gene50842.t1 | MTR_1g035640 | 27.106 | 273 | 163 | 9 | 239 | 479 | 14 | 282 | 1.26e-16 | 82.4 |
| MS.gene50842.t1 | MTR_6g061070 | 25.240 | 313 | 165 | 9 | 229 | 478 | 17 | 323 | 1.58e-16 | 82.4 |
| MS.gene50842.t1 | MTR_3g061290 | 38.793 | 116 | 67 | 2 | 229 | 344 | 11 | 122 | 4.17e-16 | 77.4 |
| MS.gene50842.t1 | MTR_1g050845 | 26.740 | 273 | 173 | 9 | 228 | 481 | 7 | 271 | 5.84e-16 | 81.3 |
| MS.gene50842.t1 | MTR_3g448730 | 37.778 | 90 | 55 | 1 | 1 | 90 | 132 | 220 | 2.70e-14 | 72.8 |
| MS.gene50842.t1 | MTR_3g061220 | 39.394 | 99 | 45 | 2 | 230 | 328 | 12 | 95 | 3.63e-14 | 69.3 |
| MS.gene50842.t1 | MTR_6g043890 | 23.592 | 284 | 170 | 7 | 229 | 467 | 14 | 295 | 5.72e-14 | 73.6 |
| MS.gene50842.t1 | MTR_4g119990 | 24.214 | 318 | 174 | 10 | 218 | 476 | 2 | 311 | 1.15e-13 | 72.8 |
| MS.gene50842.t1 | MTR_1g035480 | 31.008 | 129 | 76 | 3 | 224 | 352 | 11 | 126 | 1.81e-13 | 69.3 |
| MS.gene50842.t1 | MTR_1g035620 | 24.823 | 282 | 175 | 10 | 230 | 481 | 9 | 283 | 1.22e-12 | 70.1 |
| MS.gene50842.t1 | MTR_1g033740 | 31.967 | 122 | 78 | 3 | 229 | 350 | 21 | 137 | 1.88e-12 | 66.6 |
| MS.gene50842.t1 | MTR_6g060522 | 35.955 | 89 | 57 | 0 | 2 | 90 | 27 | 115 | 5.73e-12 | 65.1 |
| MS.gene50842.t1 | MTR_7g050640 | 34.286 | 105 | 69 | 0 | 229 | 333 | 12 | 116 | 1.02e-11 | 62.4 |
| MS.gene50842.t1 | MTR_3g093120 | 37.079 | 89 | 56 | 0 | 2 | 90 | 437 | 525 | 1.35e-11 | 67.0 |
| MS.gene50842.t1 | MTR_1g035440 | 33.654 | 104 | 68 | 1 | 229 | 331 | 14 | 117 | 1.74e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50842.t1 | AT3G18990 | 45.455 | 99 | 54 | 0 | 230 | 328 | 6 | 104 | 1.78e-22 | 99.0 |
| MS.gene50842.t1 | AT3G18990 | 34.266 | 143 | 82 | 5 | 346 | 482 | 205 | 341 | 3.96e-13 | 70.9 |
| MS.gene50842.t1 | AT3G18990 | 45.455 | 99 | 54 | 0 | 230 | 328 | 6 | 104 | 3.33e-22 | 98.6 |
| MS.gene50842.t1 | AT3G18990 | 34.722 | 144 | 82 | 5 | 346 | 483 | 205 | 342 | 2.16e-13 | 72.0 |
| MS.gene50842.t1 | AT4G01580 | 37.879 | 132 | 79 | 1 | 199 | 330 | 2 | 130 | 1.08e-20 | 90.1 |
| MS.gene50842.t1 | AT3G18960 | 37.879 | 132 | 79 | 1 | 199 | 330 | 2 | 130 | 7.88e-20 | 87.8 |
| MS.gene50842.t1 | AT3G18960 | 37.879 | 132 | 79 | 1 | 199 | 330 | 2 | 130 | 8.88e-20 | 88.2 |
| MS.gene50842.t1 | AT1G49475 | 40.187 | 107 | 63 | 1 | 228 | 334 | 32 | 137 | 7.55e-18 | 82.0 |
| MS.gene50842.t1 | AT3G18960 | 40.909 | 88 | 52 | 0 | 243 | 330 | 6 | 93 | 4.93e-15 | 73.6 |
| MS.gene50842.t1 | AT1G49480 | 35.338 | 133 | 76 | 4 | 356 | 482 | 98 | 226 | 1.16e-14 | 73.6 |
| MS.gene50842.t1 | AT1G49480 | 35.338 | 133 | 76 | 4 | 356 | 482 | 98 | 226 | 1.16e-14 | 73.6 |
| MS.gene50842.t1 | AT1G49480 | 35.338 | 133 | 76 | 4 | 356 | 482 | 98 | 226 | 1.16e-14 | 73.6 |
Find 85 sgRNAs with CRISPR-Local
Find 152 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGCTTTCCTTGATAATTT+TGG | 0.125823 | 3.4:+1019343 | None:intergenic |
| TTGTTGTCCGTTATAAAATT+AGG | 0.232666 | 3.4:+1017828 | None:intergenic |
| GATGAGTTTATAACAAGATT+TGG | 0.242784 | 3.4:-1018509 | MS.gene50842:CDS |
| TTCAAAACTTCAAGTGTTAC+TGG | 0.260689 | 3.4:-1018263 | MS.gene50842:CDS |
| GGAAAGCAGTGGGAAGTTTC+TGG | 0.267335 | 3.4:-1019330 | MS.gene50842:CDS |
| TACTATCTCTTTGTCATGAA+TGG | 0.282879 | 3.4:+1018946 | None:intergenic |
| AAATACTATTCGTTAGGATA+TGG | 0.294914 | 3.4:-1018371 | MS.gene50842:CDS |
| GGCAGCATCCTTTGATCTTT+CGG | 0.316735 | 3.4:+1019574 | None:intergenic |
| AAGTGAGTGTCATGGGAAAA+AGG | 0.323227 | 3.4:-1018189 | MS.gene50842:CDS |
| TATGGCCTTCTTGAAATGTT+TGG | 0.331141 | 3.4:+1018979 | None:intergenic |
| ACGAGTGGATTCTATAGATT+TGG | 0.332725 | 3.4:-1019270 | MS.gene50842:CDS |
| CAATGACAGAAAAGGAAGTT+AGG | 0.334106 | 3.4:-1019006 | MS.gene50842:CDS |
| TGGAGCTTTGTAGGAACATT+TGG | 0.337830 | 3.4:+1017933 | None:intergenic |
| TTCAAACTTGATAGTTGATA+AGG | 0.350720 | 3.4:+1019942 | None:intergenic |
| TCCCTTTGTATTTGAAAGAT+AGG | 0.356495 | 3.4:+1018342 | None:intergenic |
| ATTATCAACAAAGATCTTAG+TGG | 0.362204 | 3.4:+1020122 | None:intergenic |
| TCCATTGTGTAGTATGTTCC+TGG | 0.362474 | 3.4:-1019411 | MS.gene50842:intron |
| GGACTAGCACACTTTGTGTT+TGG | 0.364884 | 3.4:+1018236 | None:intergenic |
| ATGAGTTTATAACAAGATTT+GGG | 0.367534 | 3.4:-1018508 | MS.gene50842:CDS |
| TGCATAGCAGCCAAAGGCTT+TGG | 0.374814 | 3.4:+1019517 | None:intergenic |
| ATGAATGGGCGAGGGAAGTA+TGG | 0.376143 | 3.4:+1018961 | None:intergenic |
| TCAAACTTGATAGTTGATAA+GGG | 0.379437 | 3.4:+1019943 | None:intergenic |
| TGGAGCATGAACGGAACATT+TGG | 0.383147 | 3.4:+1019363 | None:intergenic |
| AGCATGTTTAGACACGCTTT+TGG | 0.391587 | 3.4:+1018166 | None:intergenic |
| GAGCTCAGACAGTTTATGTT+TGG | 0.396072 | 3.4:+1019602 | None:intergenic |
| GTGGGAAGTTTCTGGTGTAT+TGG | 0.397299 | 3.4:-1019322 | MS.gene50842:CDS |
| AATCTTGTTATAAACTCATC+TGG | 0.413404 | 3.4:+1018512 | None:intergenic |
| ACTATCTCTTTGTCATGAAT+GGG | 0.425221 | 3.4:+1018947 | None:intergenic |
| TTTGCAAAATACTATTCGTT+AGG | 0.426106 | 3.4:-1018377 | MS.gene50842:CDS |
| CAGTTCCTGATGGTCGTGAT+TGG | 0.434376 | 3.4:-1018457 | MS.gene50842:CDS |
| TATTGCAATGCAGCCTCCAC+AGG | 0.439091 | 3.4:-1019044 | MS.gene50842:intron |
| ATTGTGACTTCTAGCACAAC+CGG | 0.451587 | 3.4:+1017759 | None:intergenic |
| CAATTTGATGTTGAAATGCT+TGG | 0.454570 | 3.4:+1020053 | None:intergenic |
| ACACTCACTTGTTTCAACCT+TGG | 0.458460 | 3.4:+1018202 | None:intergenic |
| CTTCTTTGTGGTCAAGATGA+AGG | 0.461620 | 3.4:+1020145 | None:intergenic |
| ACCTATCTTTCAAATACAAA+GGG | 0.468933 | 3.4:-1018343 | MS.gene50842:CDS |
| AATGACAGAAAAGGAAGTTA+GGG | 0.489586 | 3.4:-1019005 | MS.gene50842:CDS |
| CTCTTTGTCATGAATGGGCG+AGG | 0.492559 | 3.4:+1018952 | None:intergenic |
| GTAACTTGTAACACCACAAC+AGG | 0.496207 | 3.4:+1019189 | None:intergenic |
| ACACAAAGTGTGCTAGTCCT+AGG | 0.498715 | 3.4:-1018232 | MS.gene50842:CDS |
| AGATCTTAGTGGCTTCTTTG+TGG | 0.499447 | 3.4:+1020133 | None:intergenic |
| TACCTATCTTTCAAATACAA+AGG | 0.508968 | 3.4:-1018344 | MS.gene50842:CDS |
| TTGAAACAAGTGAGTGTCAT+GGG | 0.514216 | 3.4:-1018196 | MS.gene50842:CDS |
| TTCATGCTCCAAAATTATCA+AGG | 0.518713 | 3.4:-1019351 | MS.gene50842:CDS |
| TTTCTTGAGGGAAGTGCAGA+AGG | 0.519061 | 3.4:-1020028 | MS.gene50842:CDS |
| ACAAAGCTCCAAAATTCTGA+TGG | 0.522166 | 3.4:-1017921 | MS.gene50842:CDS |
| TGCCCAGTCAATGACAGAAA+AGG | 0.524540 | 3.4:-1019014 | MS.gene50842:CDS |
| CTCTGCTTCCGAAAGATCAA+AGG | 0.527426 | 3.4:-1019582 | MS.gene50842:CDS |
| TGGGCACACCTTCCCTGTGG+AGG | 0.538744 | 3.4:+1019031 | None:intergenic |
| GTGGATTCTATAGATTTGGA+AGG | 0.539846 | 3.4:-1019266 | MS.gene50842:CDS |
| CCTCACCGCATATTTCGTCT+TGG | 0.545297 | 3.4:+1018076 | None:intergenic |
| ACAAGTTACTGCTTCTTGCA+CGG | 0.545719 | 3.4:-1019175 | MS.gene50842:intron |
| AGATCAAAGGATGCTGCCAA+TGG | 0.547942 | 3.4:-1019569 | MS.gene50842:CDS |
| ATCGTCTATGCGAATAGTGA+AGG | 0.551631 | 3.4:-1017856 | MS.gene50842:CDS |
| GGAATCCAAACATTTCAAGA+AGG | 0.552761 | 3.4:-1018984 | MS.gene50842:CDS |
| AGTGTGCTAGTCCTAGGAAA+AGG | 0.553447 | 3.4:-1018226 | MS.gene50842:CDS |
| TTGAGGGAAGTGCAGAAGGA+TGG | 0.564238 | 3.4:-1020024 | MS.gene50842:CDS |
| AAAATTATCAAGGAAAGCAG+TGG | 0.569536 | 3.4:-1019341 | MS.gene50842:CDS |
| TGTTCCTGGTGAATTTGCTA+AGG | 0.578362 | 3.4:-1019397 | MS.gene50842:CDS |
| GAAGATATGAGATCAAAGCT+TGG | 0.601885 | 3.4:-1019922 | MS.gene50842:intron |
| CAATGCAGCCTCCACAGGGA+AGG | 0.602685 | 3.4:-1019039 | MS.gene50842:intron |
| ACAACTTTGAGCTGATCAAG+AGG | 0.603297 | 3.4:-1019215 | MS.gene50842:CDS |
| AAATTATCAAGGAAAGCAGT+GGG | 0.608378 | 3.4:-1019340 | MS.gene50842:CDS |
| GCTACAGTTACAGTTCCTGA+TGG | 0.609577 | 3.4:-1018467 | MS.gene50842:CDS |
| GTTGAAACAAGTGAGTGTCA+TGG | 0.609710 | 3.4:-1018197 | MS.gene50842:CDS |
| AAATTCTGATGGAAAACAGT+GGG | 0.613196 | 3.4:-1017910 | MS.gene50842:CDS |
| TCGTCTATGCGAATAGTGAA+GGG | 0.615382 | 3.4:-1017855 | MS.gene50842:CDS |
| AAGGGAAAACAACCTATCAA+AGG | 0.616647 | 3.4:-1019247 | MS.gene50842:CDS |
| TGAGCTCATCAAGAAGAAGC+CGG | 0.617788 | 3.4:-1017778 | MS.gene50842:CDS |
| TGGATTCTATAGATTTGGAA+GGG | 0.619936 | 3.4:-1019265 | MS.gene50842:CDS |
| GACTGGGCACACCTTCCCTG+TGG | 0.620586 | 3.4:+1019028 | None:intergenic |
| AAAATTCTGATGGAAAACAG+TGG | 0.620787 | 3.4:-1017911 | MS.gene50842:CDS |
| TGCTCCTTAGCAAATTCACC+AGG | 0.628603 | 3.4:+1019393 | None:intergenic |
| CCTCACTGCATAGCAGCCAA+AGG | 0.634621 | 3.4:+1019511 | None:intergenic |
| TCAGCAATGCGAATTACGAG+TGG | 0.638532 | 3.4:-1019285 | MS.gene50842:CDS |
| TCTTTGTCATGAATGGGCGA+GGG | 0.647829 | 3.4:+1018953 | None:intergenic |
| TAGTCCTAGGAAAAGGTCCA+AGG | 0.655098 | 3.4:-1018219 | MS.gene50842:CDS |
| GATCAAGAGGAAGCCTGTTG+TGG | 0.656312 | 3.4:-1019202 | MS.gene50842:CDS |
| TCGCATAGACGATGATCTGA+CGG | 0.661216 | 3.4:+1017866 | None:intergenic |
| ACATGCTTCTACAAGAGCAG+AGG | 0.662151 | 3.4:-1018150 | MS.gene50842:CDS |
| ACGGACAACAATCTGTCACA+TGG | 0.662347 | 3.4:-1017816 | MS.gene50842:CDS |
| ATTGCAATGCAGCCTCCACA+GGG | 0.663913 | 3.4:-1019043 | MS.gene50842:intron |
| TACTTCACAGCAAAATCACA+AGG | 0.676887 | 3.4:+1017963 | None:intergenic |
| AAAATGAGATTGAAGAAACG+CGG | 0.691481 | 3.4:-1018434 | MS.gene50842:CDS |
| AATCGCCAAGACGAAATATG+CGG | 0.738605 | 3.4:-1018081 | MS.gene50842:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTTATTCTTATCATC+AGG | + | chr3.4:1019046-1019065 | None:intergenic | 15.0% |
| !!! | TTTTTTTATTCTTATCATCA+GGG | + | chr3.4:1019045-1019064 | None:intergenic | 15.0% |
| !! | ATGAGTTTATAACAAGATTT+GGG | - | chr3.4:1019394-1019413 | MS.gene50842:CDS | 20.0% |
| !! | GCAATAACTATTATCAATAA+AGG | + | chr3.4:1018837-1018856 | None:intergenic | 20.0% |
| !! | TAATTAATTGTGTTGTATTG+TGG | - | chr3.4:1018123-1018142 | MS.gene50842:CDS | 20.0% |
| !! | TGATTTACATTTATATGTTG+CGG | - | chr3.4:1018451-1018470 | MS.gene50842:CDS | 20.0% |
| !!! | AACAAAAATAAGGGTTTTTT+GGG | + | chr3.4:1019797-1019816 | None:intergenic | 20.0% |
| !!! | CGATTTTTGAACAAAAATAA+GGG | + | chr3.4:1019806-1019825 | None:intergenic | 20.0% |
| !!! | GGATTTTACTTTATAGTATT+TGG | - | chr3.4:1018412-1018431 | MS.gene50842:CDS | 20.0% |
| !!! | GGATTTTACTTTATATCTTT+TGG | - | chr3.4:1019847-1019866 | MS.gene50842:intron | 20.0% |
| ! | AAATACTATTCGTTAGGATA+TGG | - | chr3.4:1019531-1019550 | MS.gene50842:CDS | 25.0% |
| ! | ACCTATCTTTCAAATACAAA+GGG | - | chr3.4:1019559-1019578 | MS.gene50842:CDS | 25.0% |
| ! | ACTACTTCAAAACATCAAAT+TGG | - | chr3.4:1019287-1019306 | MS.gene50842:CDS | 25.0% |
| ! | ATTATCAACAAAGATCTTAG+TGG | + | chr3.4:1017783-1017802 | None:intergenic | 25.0% |
| ! | CTACTTCAAAACATCAAATT+GGG | - | chr3.4:1019288-1019307 | MS.gene50842:CDS | 25.0% |
| ! | CTATAATACCAAACTTCAAA+AGG | + | chr3.4:1019336-1019355 | None:intergenic | 25.0% |
| ! | GATGAGTTTATAACAAGATT+TGG | - | chr3.4:1019393-1019412 | MS.gene50842:CDS | 25.0% |
| ! | GCATTAAAAATATTCAGCTT+TGG | - | chr3.4:1019084-1019103 | MS.gene50842:intron | 25.0% |
| ! | TACCTATCTTTCAAATACAA+AGG | - | chr3.4:1019558-1019577 | MS.gene50842:CDS | 25.0% |
| ! | TACTTCAAAACATCAAATTG+GGG | - | chr3.4:1019289-1019308 | MS.gene50842:CDS | 25.0% |
| ! | TGTAAAAAACTTCCTTTGAT+AGG | + | chr3.4:1018670-1018689 | None:intergenic | 25.0% |
| ! | TTGTTGTCCGTTATAAAATT+AGG | + | chr3.4:1020077-1020096 | None:intergenic | 25.0% |
| ! | TTTGCAAAATACTATTCGTT+AGG | - | chr3.4:1019525-1019544 | MS.gene50842:CDS | 25.0% |
| !! | AATCTTGTTATAAACTCATC+TGG | + | chr3.4:1019393-1019412 | None:intergenic | 25.0% |
| !! | GACTTTTATGTATTATGTGT+TGG | - | chr3.4:1018065-1018084 | MS.gene50842:intron | 25.0% |
| !! | GCGATTTTTGAACAAAAATA+AGG | + | chr3.4:1019807-1019826 | None:intergenic | 25.0% |
| !! | GGGATTTCCTAATTTTATAA+CGG | - | chr3.4:1020067-1020086 | MS.gene50842:CDS | 25.0% |
| !! | TCTTTTAATATCTGAACTGT+TGG | - | chr3.4:1018800-1018819 | MS.gene50842:intron | 25.0% |
| !! | TTCAAACTTGATAGTTGATA+AGG | + | chr3.4:1017963-1017982 | None:intergenic | 25.0% |
| !!! | AACGAAAAGAAGTGTTTTTT+GGG | + | chr3.4:1018365-1018384 | None:intergenic | 25.0% |
| !!! | ACATCAAATTGTTTTTCTTG+AGG | - | chr3.4:1017861-1017880 | MS.gene50842:CDS | 25.0% |
| !!! | CATCAAATTGTTTTTCTTGA+GGG | - | chr3.4:1017862-1017881 | MS.gene50842:CDS | 25.0% |
| !!! | GAACAAAAATAAGGGTTTTT+TGG | + | chr3.4:1019798-1019817 | None:intergenic | 25.0% |
| !!! | TCAAACTTGATAGTTGATAA+GGG | + | chr3.4:1017962-1017981 | None:intergenic | 25.0% |
| AAAATGAGATTGAAGAAACG+CGG | - | chr3.4:1019468-1019487 | MS.gene50842:intron | 30.0% | |
| AAAATTATCAAGGAAAGCAG+TGG | - | chr3.4:1018561-1018580 | MS.gene50842:intron | 30.0% | |
| AAAATTCTGATGGAAAACAG+TGG | - | chr3.4:1019991-1020010 | MS.gene50842:CDS | 30.0% | |
| AAATTATCAAGGAAAGCAGT+GGG | - | chr3.4:1018562-1018581 | MS.gene50842:intron | 30.0% | |
| AAATTCTGATGGAAAACAGT+GGG | - | chr3.4:1019992-1020011 | MS.gene50842:CDS | 30.0% | |
| AATACCAAACTTCAAAAGGA+GGG | + | chr3.4:1019332-1019351 | None:intergenic | 30.0% | |
| AATGACAGAAAAGGAAGTTA+GGG | - | chr3.4:1018897-1018916 | MS.gene50842:intron | 30.0% | |
| AATGGATGAGATCAATTTAG+TGG | + | chr3.4:1019907-1019926 | None:intergenic | 30.0% | |
| ACTATCTCTTTGTCATGAAT+GGG | + | chr3.4:1018958-1018977 | None:intergenic | 30.0% | |
| CAATTTGATGTTGAAATGCT+TGG | + | chr3.4:1017852-1017871 | None:intergenic | 30.0% | |
| GAGTGTTAAATATCATGCTT+AGG | - | chr3.4:1018028-1018047 | MS.gene50842:intron | 30.0% | |
| TAATACCAAACTTCAAAAGG+AGG | + | chr3.4:1019333-1019352 | None:intergenic | 30.0% | |
| TACTATCTCTTTGTCATGAA+TGG | + | chr3.4:1018959-1018978 | None:intergenic | 30.0% | |
| TCCCTTTGTATTTGAAAGAT+AGG | + | chr3.4:1019563-1019582 | None:intergenic | 30.0% | |
| TGGATTCTATAGATTTGGAA+GGG | - | chr3.4:1018637-1018656 | MS.gene50842:intron | 30.0% | |
| TGTTGTATTGTGGTAGTTAT+TGG | - | chr3.4:1018133-1018152 | MS.gene50842:CDS | 30.0% | |
| TTCAAAACTTCAAGTGTTAC+TGG | - | chr3.4:1019639-1019658 | MS.gene50842:intron | 30.0% | |
| TTCATGCTCCAAAATTATCA+AGG | - | chr3.4:1018551-1018570 | MS.gene50842:intron | 30.0% | |
| ! | ACATTTTCTTTTGCAACGAA+TGG | - | chr3.4:1019496-1019515 | MS.gene50842:intron | 30.0% |
| ! | ACTGTTTTCCATCAGAATTT+TGG | + | chr3.4:1019992-1020011 | None:intergenic | 30.0% |
| ! | TCTCATAATCCAGTTTTTCA+CGG | - | chr3.4:1017821-1017840 | MS.gene50842:CDS | 30.0% |
| !! | ACTGCTTTCCTTGATAATTT+TGG | + | chr3.4:1018562-1018581 | None:intergenic | 30.0% |
| !!! | GAACGAAAAGAAGTGTTTTT+TGG | + | chr3.4:1018366-1018385 | None:intergenic | 30.0% |
| !!! | GTAACACTTGAAGTTTTGAA+TGG | + | chr3.4:1019639-1019658 | None:intergenic | 30.0% |
| !!! | GTTTTTTGGGTTGAATTCAT+TGG | + | chr3.4:1019784-1019803 | None:intergenic | 30.0% |
| !!! | TGATAATTTTGGAGCATGAA+CGG | + | chr3.4:1018551-1018570 | None:intergenic | 30.0% |
| AAGGGAAAACAACCTATCAA+AGG | - | chr3.4:1018655-1018674 | MS.gene50842:intron | 35.0% | |
| ACAAAGCTCCAAAATTCTGA+TGG | - | chr3.4:1019981-1020000 | MS.gene50842:CDS | 35.0% | |
| CAATGACAGAAAAGGAAGTT+AGG | - | chr3.4:1018896-1018915 | MS.gene50842:intron | 35.0% | |
| GAAGATATGAGATCAAAGCT+TGG | - | chr3.4:1017980-1017999 | MS.gene50842:intron | 35.0% | |
| GGAATCCAAACATTTCAAGA+AGG | - | chr3.4:1018918-1018937 | MS.gene50842:intron | 35.0% | |
| GTGGATTCTATAGATTTGGA+AGG | - | chr3.4:1018636-1018655 | MS.gene50842:intron | 35.0% | |
| TACTTCACAGCAAAATCACA+AGG | + | chr3.4:1019942-1019961 | None:intergenic | 35.0% | |
| TATGGCCTTCTTGAAATGTT+TGG | + | chr3.4:1018926-1018945 | None:intergenic | 35.0% | |
| TCCAAGTTAGAACTGAAACT+AGG | - | chr3.4:1019196-1019215 | MS.gene50842:CDS | 35.0% | |
| TCTAACTTGGAGGTTGTATT+TGG | + | chr3.4:1019187-1019206 | None:intergenic | 35.0% | |
| TTGAAACAAGTGAGTGTCAT+GGG | - | chr3.4:1019706-1019725 | MS.gene50842:intron | 35.0% | |
| TTGAACAAGAAAACAGTTGC+AGG | + | chr3.4:1018268-1018287 | None:intergenic | 35.0% | |
| TTGTCAATGTCTCTGTGATT+TGG | - | chr3.4:1019109-1019128 | MS.gene50842:intron | 35.0% | |
| ! | AAACTGTGTCATTGGATTGT+TGG | - | chr3.4:1019150-1019169 | MS.gene50842:intron | 35.0% |
| ! | ACGAGTGGATTCTATAGATT+TGG | - | chr3.4:1018632-1018651 | MS.gene50842:intron | 35.0% |
| ! | TAGTTTCAGTTCTAACTTGG+AGG | + | chr3.4:1019197-1019216 | None:intergenic | 35.0% |
| !! | TTCCTTTTCTGTCATTGACT+GGG | + | chr3.4:1018893-1018912 | None:intergenic | 35.0% |
| !! | TTGGTTTTTCCGTGAAAAAC+TGG | + | chr3.4:1017833-1017852 | None:intergenic | 35.0% |
| !!! | ATTCGCATTGCTGATTTTCT+TGG | + | chr3.4:1018611-1018630 | None:intergenic | 35.0% |
| !!! | GTTTTTTGGGTTGATTCCAT+TGG | + | chr3.4:1018352-1018371 | None:intergenic | 35.0% |
| !!! | TCAGAATTTTGGAGCTTTGT+AGG | + | chr3.4:1019981-1020000 | None:intergenic | 35.0% |
| AAGTGAGTGTCATGGGAAAA+AGG | - | chr3.4:1019713-1019732 | MS.gene50842:intron | 40.0% | |
| AATCGCCAAGACGAAATATG+CGG | - | chr3.4:1019821-1019840 | MS.gene50842:intron | 40.0% | |
| ACAACTTTGAGCTGATCAAG+AGG | - | chr3.4:1018687-1018706 | MS.gene50842:intron | 40.0% | |
| ACAAGGAACATACTGCACAA+TGG | + | chr3.4:1019925-1019944 | None:intergenic | 40.0% | |
| ACAAGTTACTGCTTCTTGCA+CGG | - | chr3.4:1018727-1018746 | MS.gene50842:intron | 40.0% | |
| ACACTCACTTGTTTCAACCT+TGG | + | chr3.4:1019703-1019722 | None:intergenic | 40.0% | |
| ACCAGGAACATACTACACAA+TGG | + | chr3.4:1018495-1018514 | None:intergenic | 40.0% | |
| AGATCTTAGTGGCTTCTTTG+TGG | + | chr3.4:1017772-1017791 | None:intergenic | 40.0% | |
| AGGAACATACTACACAATGG+AGG | + | chr3.4:1018492-1018511 | None:intergenic | 40.0% | |
| ATCGTCTATGCGAATAGTGA+AGG | - | chr3.4:1020046-1020065 | MS.gene50842:CDS | 40.0% | |
| ATGACTCGAAACTGTGTCAT+TGG | - | chr3.4:1019142-1019161 | MS.gene50842:intron | 40.0% | |
| ATTGGATTGTTGGAGACGAA+AGG | - | chr3.4:1019160-1019179 | MS.gene50842:intron | 40.0% | |
| ATTGTGACTTCTAGCACAAC+CGG | + | chr3.4:1020146-1020165 | None:intergenic | 40.0% | |
| CTTCTTTGTGGTCAAGATGA+AGG | + | chr3.4:1017760-1017779 | None:intergenic | 40.0% | |
| GACAGCAACTCAAAACAGAA+AGG | + | chr3.4:1018988-1019007 | None:intergenic | 40.0% | |
| GAGCTCAGACAGTTTATGTT+TGG | + | chr3.4:1018303-1018322 | None:intergenic | 40.0% | |
| GCAGGAAATGAACAAGACAA+AGG | + | chr3.4:1018250-1018269 | None:intergenic | 40.0% | |
| GTTGAAACAAGTGAGTGTCA+TGG | - | chr3.4:1019705-1019724 | MS.gene50842:intron | 40.0% | |
| TCCATTGTGTAGTATGTTCC+TGG | - | chr3.4:1018491-1018510 | MS.gene50842:CDS | 40.0% | |
| TCGTCTATGCGAATAGTGAA+GGG | - | chr3.4:1020047-1020066 | MS.gene50842:CDS | 40.0% | |
| TTAACACTCCTCCTTGCTTT+AGG | + | chr3.4:1018017-1018036 | None:intergenic | 40.0% | |
| ! | AGCATGTTTAGACACGCTTT+TGG | + | chr3.4:1019739-1019758 | None:intergenic | 40.0% |
| ! | ATTTTCCAATCACGACCATC+AGG | + | chr3.4:1019453-1019472 | None:intergenic | 40.0% |
| ! | CTTCCTTTTCTGTCATTGAC+TGG | + | chr3.4:1018894-1018913 | None:intergenic | 40.0% |
| ! | GCCTAGTTTCAGTTCTAACT+TGG | + | chr3.4:1019200-1019219 | None:intergenic | 40.0% |
| ! | GTAACTTGTAACACCACAAC+AGG | + | chr3.4:1018716-1018735 | None:intergenic | 40.0% |
| ! | TCTTTTCGTTCCAAAGCCTT+TGG | - | chr3.4:1018375-1018394 | MS.gene50842:CDS | 40.0% |
| ! | TGGAGCTTTGTAGGAACATT+TGG | + | chr3.4:1019972-1019991 | None:intergenic | 40.0% |
| !! | TGTTCCTGGTGAATTTGCTA+AGG | - | chr3.4:1018505-1018524 | MS.gene50842:CDS | 40.0% |
| !!! | GTTACCCTCCTTTTGAAGTT+TGG | - | chr3.4:1019325-1019344 | MS.gene50842:CDS | 40.0% |
| ACACAAAGTGTGCTAGTCCT+AGG | - | chr3.4:1019670-1019689 | MS.gene50842:intron | 45.0% | |
| ACATGCTTCTACAAGAGCAG+AGG | - | chr3.4:1019752-1019771 | MS.gene50842:intron | 45.0% | |
| ACGGACAACAATCTGTCACA+TGG | - | chr3.4:1020086-1020105 | MS.gene50842:CDS | 45.0% | |
| AGATCAAAGGATGCTGCCAA+TGG | - | chr3.4:1018333-1018352 | MS.gene50842:CDS | 45.0% | |
| AGTGTGCTAGTCCTAGGAAA+AGG | - | chr3.4:1019676-1019695 | MS.gene50842:intron | 45.0% | |
| CTCTGCTTCCGAAAGATCAA+AGG | - | chr3.4:1018320-1018339 | MS.gene50842:CDS | 45.0% | |
| CTGCAGTGATAACAGCTCAT+TGG | + | chr3.4:1019364-1019383 | None:intergenic | 45.0% | |
| GCTACAGTTACAGTTCCTGA+TGG | - | chr3.4:1019435-1019454 | MS.gene50842:intron | 45.0% | |
| GGCAGCATCCTTTGATCTTT+CGG | + | chr3.4:1018331-1018350 | None:intergenic | 45.0% | |
| TAGTCCTAGGAAAAGGTCCA+AGG | - | chr3.4:1019683-1019702 | MS.gene50842:intron | 45.0% | |
| TCAGCAATGCGAATTACGAG+TGG | - | chr3.4:1018617-1018636 | MS.gene50842:intron | 45.0% | |
| TCGCATAGACGATGATCTGA+CGG | + | chr3.4:1020039-1020058 | None:intergenic | 45.0% | |
| TCTTTGTCATGAATGGGCGA+GGG | + | chr3.4:1018952-1018971 | None:intergenic | 45.0% | |
| TGAGCTCATCAAGAAGAAGC+CGG | - | chr3.4:1020124-1020143 | MS.gene50842:CDS | 45.0% | |
| TGCCCAGTCAATGACAGAAA+AGG | - | chr3.4:1018888-1018907 | MS.gene50842:intron | 45.0% | |
| TGCTCCTTAGCAAATTCACC+AGG | + | chr3.4:1018512-1018531 | None:intergenic | 45.0% | |
| TGGAGCATGAACGGAACATT+TGG | + | chr3.4:1018542-1018561 | None:intergenic | 45.0% | |
| TGTTTGCAGCTCCTAAAGCA+AGG | - | chr3.4:1018003-1018022 | MS.gene50842:intron | 45.0% | |
| ! | GGACTAGCACACTTTGTGTT+TGG | + | chr3.4:1019669-1019688 | None:intergenic | 45.0% |
| ! | TCAACCTTGGACCTTTTCCT+AGG | + | chr3.4:1019690-1019709 | None:intergenic | 45.0% |
| ! | TTTCTTGAGGGAAGTGCAGA+AGG | - | chr3.4:1017874-1017893 | MS.gene50842:CDS | 45.0% |
| !! | GTGGGAAGTTTCTGGTGTAT+TGG | - | chr3.4:1018580-1018599 | MS.gene50842:intron | 45.0% |
| !! | TGAGAGCACTTAGACGTGAT+TGG | - | chr3.4:1019235-1019254 | MS.gene50842:CDS | 45.0% |
| !!! | AGTGGGAAGTGTTTTGCATG+CGG | - | chr3.4:1020009-1020028 | MS.gene50842:CDS | 45.0% |
| ATGAATGGGCGAGGGAAGTA+TGG | + | chr3.4:1018944-1018963 | None:intergenic | 50.0% | |
| ATTGCAATGCAGCCTCCACA+GGG | - | chr3.4:1018859-1018878 | MS.gene50842:intron | 50.0% | |
| CAGTTCCTGATGGTCGTGAT+TGG | - | chr3.4:1019445-1019464 | MS.gene50842:intron | 50.0% | |
| CCAAGACGAAATATGCGGTG+AGG | - | chr3.4:1019826-1019845 | MS.gene50842:intron | 50.0% | |
| CCTCACCGCATATTTCGTCT+TGG | + | chr3.4:1019829-1019848 | None:intergenic | 50.0% | |
| CTCTTTGTCATGAATGGGCG+AGG | + | chr3.4:1018953-1018972 | None:intergenic | 50.0% | |
| GATCAAGAGGAAGCCTGTTG+TGG | - | chr3.4:1018700-1018719 | MS.gene50842:intron | 50.0% | |
| GGAAAGCAGTGGGAAGTTTC+TGG | - | chr3.4:1018572-1018591 | MS.gene50842:intron | 50.0% | |
| TATTGCAATGCAGCCTCCAC+AGG | - | chr3.4:1018858-1018877 | MS.gene50842:intron | 50.0% | |
| TGCATAGCAGCCAAAGGCTT+TGG | + | chr3.4:1018388-1018407 | None:intergenic | 50.0% | |
| TTGCAGCTCCTAAAGCAAGG+AGG | - | chr3.4:1018006-1018025 | MS.gene50842:intron | 50.0% | |
| ! | TTGAGGGAAGTGCAGAAGGA+TGG | - | chr3.4:1017878-1017897 | MS.gene50842:CDS | 50.0% |
| CCTCACTGCATAGCAGCCAA+AGG | + | chr3.4:1018394-1018413 | None:intergenic | 55.0% | |
| CCTTTGGCTGCTATGCAGTG+AGG | - | chr3.4:1018391-1018410 | MS.gene50842:CDS | 55.0% | |
| CAATGCAGCCTCCACAGGGA+AGG | - | chr3.4:1018863-1018882 | MS.gene50842:intron | 60.0% | |
| GACTGGGCACACCTTCCCTG+TGG | + | chr3.4:1018877-1018896 | None:intergenic | 65.0% | |
| TGGGCACACCTTCCCTGTGG+AGG | + | chr3.4:1018874-1018893 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 1017731 | 1020193 | 1017731 | ID=MS.gene50842 |
| chr3.4 | mRNA | 1017731 | 1020193 | 1017731 | ID=MS.gene50842.t1;Parent=MS.gene50842 |
| chr3.4 | exon | 1019923 | 1020193 | 1019923 | ID=MS.gene50842.t1.exon1;Parent=MS.gene50842.t1 |
| chr3.4 | CDS | 1019923 | 1020193 | 1019923 | ID=cds.MS.gene50842.t1;Parent=MS.gene50842.t1 |
| chr3.4 | exon | 1019517 | 1019626 | 1019517 | ID=MS.gene50842.t1.exon2;Parent=MS.gene50842.t1 |
| chr3.4 | CDS | 1019517 | 1019626 | 1019517 | ID=cds.MS.gene50842.t1;Parent=MS.gene50842.t1 |
| chr3.4 | exon | 1019176 | 1019421 | 1019176 | ID=MS.gene50842.t1.exon3;Parent=MS.gene50842.t1 |
| chr3.4 | CDS | 1019176 | 1019421 | 1019176 | ID=cds.MS.gene50842.t1;Parent=MS.gene50842.t1 |
| chr3.4 | exon | 1018949 | 1019053 | 1018949 | ID=MS.gene50842.t1.exon4;Parent=MS.gene50842.t1 |
| chr3.4 | CDS | 1018949 | 1019053 | 1018949 | ID=cds.MS.gene50842.t1;Parent=MS.gene50842.t1 |
| chr3.4 | exon | 1018082 | 1018540 | 1018082 | ID=MS.gene50842.t1.exon5;Parent=MS.gene50842.t1 |
| chr3.4 | CDS | 1018082 | 1018540 | 1018082 | ID=cds.MS.gene50842.t1;Parent=MS.gene50842.t1 |
| chr3.4 | exon | 1017731 | 1017991 | 1017731 | ID=MS.gene50842.t1.exon6;Parent=MS.gene50842.t1 |
| chr3.4 | CDS | 1017731 | 1017991 | 1017731 | ID=cds.MS.gene50842.t1;Parent=MS.gene50842.t1 |
| Gene Sequence |
| Protein sequence |
