AlfalfaGEDB | MS.gene50846

M. sativa cultivar XinJiangDaYe / MS.gene50846

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50846.t1	AES68281.1	81.4	274	41	1	1	274	1	264	1.70E-123	452.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50846.t1	Q8L3W1	27.9	341	153	8	21	274	6	340	7.0e-25	115.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50846.t1	G7IYE9	81.4	274	41	1	1	274	1	264	1.2e-123	452.2

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene50846.t1	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50846.t1	MTR_3g005480	83.942	274	34	1	1	274	1	264	3.53e-171	474
MS.gene50846.t1	MTR_3g005490	78.261	276	49	2	1	276	1	265	1.32e-156	437
MS.gene50846.t1	MTR_5g070440	69.203	276	83	1	1	276	1	274	1.95e-138	391
MS.gene50846.t1	MTR_3g005420	64.368	261	91	1	16	276	284	542	1.97e-117	347
MS.gene50846.t1	MTR_3g005420	54.275	269	97	4	1	267	20	264	8.43e-84	261
MS.gene50846.t1	MTR_3g005460	62.132	272	94	3	14	276	252	523	1.35e-111	332
MS.gene50846.t1	MTR_3g005460	54.717	265	93	4	2	264	4	243	1.99e-87	270
MS.gene50846.t1	MTR_3g005420	54.023	261	83	2	16	276	265	488	2.36e-89	274
MS.gene50846.t1	MTR_3g005420	54.275	269	97	4	1	267	1	245	1.31e-84	261
MS.gene50846.t1	MTR_3g005440	59.821	224	83	1	5	228	226	442	4.63e-85	261

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50846.t1	AT3G18990	41.667	120	66	1	21	140	6	121	6.42e-25	102
MS.gene50846.t1	AT3G18990	36.296	135	74	4	147	275	213	341	1.13e-15	76.3
MS.gene50846.t1	AT3G18990	41.667	120	66	1	21	140	6	121	1.64e-24	101
MS.gene50846.t1	AT3G18990	36.029	136	75	4	147	276	213	342	2.70e-15	75.5
MS.gene50846.t1	AT1G49475	41.667	120	69	1	10	129	23	141	2.11e-23	94.7
MS.gene50846.t1	AT3G18960	36.975	119	72	1	3	121	15	130	3.31e-21	89.0
MS.gene50846.t1	AT3G18960	36.975	119	72	1	3	121	15	130	4.27e-21	89.0
MS.gene50846.t1	AT4G01580	37.383	107	67	0	15	121	24	130	9.97e-21	87.4
MS.gene50846.t1	AT1G49480	36.296	135	74	4	147	275	98	226	4.89e-16	75.5
MS.gene50846.t1	AT1G49480	36.296	135	74	4	147	275	98	226	4.89e-16	75.5

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Find 50 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCTGAGGGAGAGCAT+TGG	0.523796	3.4:-1072103	MS.gene50846:CDS
AAATCTTTCTCCGCCGCCGG+TGG	0.575868	3.4:+1073725	None:intergenic
AAATTCTGAGGGAGAGCATT+GGG	0.443772	3.4:-1072102	MS.gene50846:CDS
AACAAACAATGCAGGCTCGC+CGG	0.535855	3.4:-1073763	None:intergenic
AAGCTCTGTCCTGCCACCGG+CGG	0.650688	3.4:-1073738	MS.gene50846:CDS
AAGTGAGAGTCATGAGAAAA+AGG	0.253305	3.4:-1072369	MS.gene50846:CDS
AATCTTGTTATAAACTCATC+TGG	0.413404	3.4:+1072692	None:intergenic
AATGCTCTCCCTCAGAATTT+TGG	0.165314	3.4:+1072105	None:intergenic
AATGTGCAGTATGTTAATGC+TGG	0.551027	3.4:-1072170	MS.gene50846:intron
AATTCTAATGGGAAATTATG+TGG	0.499901	3.4:-1072261	MS.gene50846:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTGAACCAAATCGAAACT+AGG	-	chr3.4:1072854-1072873	MS.gene50846:intron	30.0%
AGTCTCTTTATGATTGTTTG+GGG	-	chr3.4:1072638-1072657	MS.gene50846:CDS	30.0%
ATGATGACATGTTCTGTTTA+AGG	-	chr3.4:1072732-1072751	MS.gene50846:intron	30.0%
CATCTGCAATGATAATATCA+AGG	-	chr3.4:1073629-1073648	MS.gene50846:intron	30.0%
GAATACTATTCTATAGGGTA+TGG	-	chr3.4:1073121-1073140	MS.gene50846:intron	30.0%
GGGGATTTACTATATTTCAA+AGG	-	chr3.4:1073650-1073669	MS.gene50846:intron	30.0%
TCCTGTGTTTCAAATATGAA+GGG	-	chr3.4:1073149-1073168	MS.gene50846:intron	30.0%
TCTGCAATGATAATATCAAG+GGG	-	chr3.4:1073631-1073650	MS.gene50846:intron	30.0%
TTCCTGTGTTTCAAATATGA+AGG	-	chr3.4:1073148-1073167	MS.gene50846:intron	30.0%
TTGAATTGAAAGTGACTACA+TGG	-	chr3.4:1072525-1072544	MS.gene50846:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	1071917	1073777	1071917	ID=MS.gene50846
chr3.4	mRNA	1071917	1073777	1071917	ID=MS.gene50846.t1;Parent=MS.gene50846
chr3.4	exon	1073673	1073777	1073673	ID=MS.gene50846.t1.exon1;Parent=MS.gene50846.t1
chr3.4	CDS	1073673	1073777	1073673	ID=cds.MS.gene50846.t1;Parent=MS.gene50846.t1
chr3.4	exon	1072262	1072720	1072262	ID=MS.gene50846.t1.exon2;Parent=MS.gene50846.t1
chr3.4	CDS	1072262	1072720	1072262	ID=cds.MS.gene50846.t1;Parent=MS.gene50846.t1
chr3.4	exon	1071917	1072183	1071917	ID=MS.gene50846.t1.exon3;Parent=MS.gene50846.t1
chr3.4	CDS	1071917	1072183	1071917	ID=cds.MS.gene50846.t1;Parent=MS.gene50846.t1

Gene Sequence

>MS.gene50846

ATGCAGGCTCGCCGGAGAAGCTCTGTCCTGCCACCGGCGGCGGAGAAAGATTTCAAACATTTTATGAAAGTCATACTTCCCTCACCCATTCATGGCACTCAATTAGTAAGTTTATGTATCTGTCTTTCTTTTGAGTTGTTTTTCTGCCTATATATGTTTATGTCTCAAATAGTAGTGTTTTTTCTGGCTTGTTTTGTACTATTATATATGCATGCATGCATGCATGTCAATCAAATATTATTAATTTTATAAAATCATCAAATGTGTTTGTGCCGAAACTCTCTTTGCCATTGCTTTTGTGAATCTGGTTTAGATGCTTTGTGACTCTTTTGTGTGTGCTTGCATGTTGTGTTGATCGCTTGTTCTGTTTAGTGTTTCTTTCATTCAAAGGTTGTCAAAATTGATTTTTAATCTCTTATTGAGAGTTAGTTTAGTGTATGTTTGGTTTCACTTTTGGATAAGGCAAAATTGATTCTAGAGATGTGAAATTGATTTTGACATCTTTGCATGTGTTTGAGTAGAATTGATTTTGTCTCCTTAATTGTTTTTAATATGAAGCTAGAATTAGGAATTTTTGTCTCTACATATGTTTGATTTCATTCCCATAGTTGAATTGAAAGTGACTACATGGTGGTACATACTTAGTTGAATCAGAAGTGACTTGATTACATTCTTAGTTTAATTTGTGAATTGTGGGTAGCTTGTTTTGTGCTTCCTCGAAAGTCTCTTTATGATTGTTTGGGGAATCTGTTTGAAATGCTTTGTGACTCTTTTGCATGATTCGATTTCGACATTCAGTGCTCTCTCTATTCAAGATGATGACATGTTCTGTTTAAGGTTTATCCTTCCTTTTTCTACTAAGTTTACTTATCTTCGTTTGGTTCTATGGCGACAAAAATTTTGAACAAATTGAAGAAAAGTGAGCTGACTCTTAATTAATTGAACCAAATCGAAACTAGGAGCAATAAAAGTAAACCAATTATGTAAATCTCAAATGTGTCAGCCCTCCTCTAAAATTTGGTCCTATACTATAACGGATGAACTGTTATCACTGCAGAGAATTCCAGATGAGTTTATAACAAGATTTGGACATGAACTCAAGAATGTTGCTACAATTACTGTTCCTGATGGTCGTGATTGGAGAATGGAACTGAAGAAATGCGGCAACGACGTTTACTTTTGTAACAAATGGCAACAGTTTGCAGAATACTATTCTATAGGGTATGGTTCCTTCCTGTGTTTCAAATATGAAGGGGATTCAAAGTTTAGTGTCGTTATATTTGATGTAACTTCTGTTGAGATTTGCTATCCATTCAAAACTCAAAGTACAAATGGAGAAACAAACACAAAGTGTCATAGTCGTAGAAAAAGGTCCAAGGTTGAAACAAGTGAGAGTCATGAGAAAAAGGGCAAAAGCATGTCTAAACATGCTTCTGAAAGAGCAAAGGAAGCTGCCAAAGAATTTAATCCAAAAAACCCTTATTTTTGTTCCAAAATTCTAATGGGAAATTATGTGGTGAGGATTTTACTTTAAAGCAAATGTTGAGTTTTTTATTTATGTTATCACTTTAATGATCTCTCATTTAATGTGCAGTATGTTAATGCTGGTTTTGCTTCAAAGCATCTGAATCTAAATGTTCCTGTAAAGCTCCAAAATTCTGAGGGAGAGCATTGGGAAGTTTCTTGTGCAATGCATGATGCCAAATCATCATCATCTGCAATGATAATATCAAGGGGATTTACTATATTTCAAAGGGGCAACAATCTGTCTTATGGAGATTATTGTGTGTTTGAGCTGATCAATAAGAAGCCGGATGTGCTAAAAGTCACAATGTTTCGTGTGATTGACTATTGCGATTAA

Protein sequence

>MS.gene50846.t1

MQARRRSSVLPPAAEKDFKHFMKVILPSPIHGTQLRIPDEFITRFGHELKNVATITVPDGRDWRMELKKCGNDVYFCNKWQQFAEYYSIGYGSFLCFKYEGDSKFSVVIFDVTSVEICYPFKTQSTNGETNTKCHSRRKRSKVETSESHEKKGKSMSKHASERAKEAAKEFNPKNPYFCSKILMGNYVYVNAGFASKHLNLNVPVKLQNSEGEHWEVSCAMHDAKSSSSAMIISRGFTIFQRGNNLSYGDYCVFELINKKPDVLKVTMFRVIDYCD