Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51013.t1 | XP_013459385.1 | 100 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 2.20E-38 | 167.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51013.t1 | Q9CA64 | 75.3 | 93 | 22 | 1 | 1 | 92 | 1 | 93 | 1.0e-28 | 127.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51013.t1 | I3STA1 | 100.0 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 1.6e-38 | 167.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene51013.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51013.t1 | MTR_3g438070 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 3.41e-60 | 178 |
| MS.gene51013.t1 | MTR_7g010120 | 72.340 | 94 | 23 | 2 | 1 | 92 | 1 | 93 | 9.01e-38 | 122 |
| MS.gene51013.t1 | MTR_5g039180 | 72.727 | 77 | 19 | 1 | 17 | 91 | 17 | 93 | 2.66e-33 | 110 |
| MS.gene51013.t1 | MTR_6g011670 | 73.077 | 78 | 19 | 1 | 17 | 92 | 15 | 92 | 1.58e-32 | 108 |
| MS.gene51013.t1 | MTR_7g059260 | 48.611 | 72 | 37 | 0 | 8 | 79 | 3 | 74 | 2.92e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51013.t1 | AT3G47710 | 73.118 | 93 | 23 | 2 | 1 | 92 | 1 | 92 | 2.65e-39 | 125 |
| MS.gene51013.t1 | AT1G74500 | 77.922 | 77 | 16 | 1 | 17 | 92 | 17 | 93 | 9.44e-36 | 117 |
| MS.gene51013.t1 | AT1G26945 | 79.730 | 74 | 13 | 1 | 19 | 90 | 19 | 92 | 1.49e-34 | 114 |
| MS.gene51013.t1 | AT3G28857 | 65.957 | 94 | 27 | 3 | 1 | 91 | 1 | 92 | 1.45e-32 | 108 |
| MS.gene51013.t1 | AT5G39860 | 69.231 | 78 | 21 | 2 | 17 | 91 | 15 | 92 | 9.71e-30 | 102 |
| MS.gene51013.t1 | AT5G15160 | 66.667 | 75 | 22 | 2 | 19 | 90 | 18 | 92 | 2.48e-27 | 95.9 |
Find 17 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCTTCAAGGGTGTTGC+AGG | 0.328305 | 3.2:-30436836 | MS.gene51013:CDS |
| TGAGCGTTTGTCTGAGCTAT+TGG | 0.392203 | 3.2:-30436764 | MS.gene51013:CDS |
| TATATCAGGTTTCAGCTTCA+AGG | 0.403696 | 3.2:-30436846 | MS.gene51013:intron |
| ACTATATTAGAAACTTGAAC+AGG | 0.414262 | 3.2:-30436804 | MS.gene51013:CDS |
| AGGAAGAAGTTGCTGCAACT+TGG | 0.461097 | 3.2:+30437732 | None:intergenic |
| GAGCTCCTATTGCGAATCTC+AGG | 0.466230 | 3.2:+30437712 | None:intergenic |
| TTCTTCCTGAGATTCGCAAT+AGG | 0.484861 | 3.2:-30437717 | MS.gene51013:CDS |
| ATATCAGGTTTCAGCTTCAA+GGG | 0.502020 | 3.2:-30436845 | MS.gene51013:intron |
| CTAGCAGGAGGTCGCGGTCA+CGG | 0.548676 | 3.2:-30437807 | MS.gene51013:CDS |
| GCTCAGACAAACGCTCACTT+AGG | 0.559789 | 3.2:+30436770 | None:intergenic |
| GCAACTTGGAGACAAGATCA+TGG | 0.561243 | 3.2:+30437746 | None:intergenic |
| AGATGTCTAGCAGGAGGTCG+CGG | 0.564343 | 3.2:-30437813 | MS.gene51013:CDS |
| CACGGCAAACAAGTAGTTCG+AGG | 0.585931 | 3.2:-30437789 | MS.gene51013:CDS |
| TCGCAATAGGAGCTCTGACA+AGG | 0.593557 | 3.2:-30437704 | MS.gene51013:intron |
| AAGATCATGGATCTGATCGT+CGG | 0.643584 | 3.2:+30437759 | None:intergenic |
| TTATAAAGATGTCTAGCAGG+AGG | 0.648667 | 3.2:-30437819 | None:intergenic |
| CTATATTAGAAACTTGAACA+GGG | 0.660572 | 3.2:-30436803 | MS.gene51013:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAAAAACTTGAA+AGG | - | chr3.2:30437388-30437407 | MS.gene51013:intron | 10.0% |
| !! | TGTATTAAAAATATAGTATA+TGG | + | chr3.2:30437065-30437084 | None:intergenic | 10.0% |
| !!! | AGTTTTTTTTTTTTTTTTCT+TGG | + | chr3.2:30437384-30437403 | None:intergenic | 10.0% |
| !!! | TTATTTTTTATAAAAGTCAT+TGG | - | chr3.2:30436971-30436990 | MS.gene51013:intron | 10.0% |
| !!! | AAATTGAATTTTACTATATC+AGG | - | chr3.2:30437662-30437681 | MS.gene51013:intron | 15.0% |
| ! | ACTATATTAGAAACTTGAAC+AGG | - | chr3.2:30437718-30437737 | MS.gene51013:CDS | 25.0% |
| ! | ATTTAAACCAACTTAATCCT+AGG | - | chr3.2:30437476-30437495 | MS.gene51013:intron | 25.0% |
| ! | CTAGGAAACAAATAATATGT+AGG | + | chr3.2:30436850-30436869 | None:intergenic | 25.0% |
| ! | CTATATTAGAAACTTGAACA+GGG | - | chr3.2:30437719-30437738 | MS.gene51013:CDS | 25.0% |
| ! | GATAATGCATAATATATGCA+GGG | - | chr3.2:30437291-30437310 | MS.gene51013:intron | 25.0% |
| !!! | AGAAGAGATTAGTTTTATAG+CGG | - | chr3.2:30437086-30437105 | MS.gene51013:intron | 25.0% |
| !!! | CTAGTCTTTTGATTAAAACT+AGG | + | chr3.2:30436868-30436887 | None:intergenic | 25.0% |
| ACACTAGAGTAAGTATAAGA+GGG | - | chr3.2:30437452-30437471 | MS.gene51013:intron | 30.0% | |
| ACATCAACAATTCTTGTAGA+TGG | + | chr3.2:30437588-30437607 | None:intergenic | 30.0% | |
| ACCATACTTATTCACCTTTA+AGG | - | chr3.2:30437335-30437354 | MS.gene51013:intron | 30.0% | |
| ACCTTAAAGGTGAATAAGTA+TGG | + | chr3.2:30437339-30437358 | None:intergenic | 30.0% | |
| CAAGAAAAAGGAACAACAAT+AGG | - | chr3.2:30437512-30437531 | MS.gene51013:intron | 30.0% | |
| CATCAACAATTCTTGTAGAT+GGG | + | chr3.2:30437587-30437606 | None:intergenic | 30.0% | |
| GGATAATGCATAATATATGC+AGG | - | chr3.2:30437290-30437309 | MS.gene51013:intron | 30.0% | |
| TACACTAGAGTAAGTATAAG+AGG | - | chr3.2:30437451-30437470 | MS.gene51013:intron | 30.0% | |
| AATAGGCCTAACAAATACGA+GGG | - | chr3.2:30437529-30437548 | MS.gene51013:intron | 35.0% | |
| ATATCAGGTTTCAGCTTCAA+GGG | - | chr3.2:30437677-30437696 | MS.gene51013:intron | 35.0% | |
| GAGATACCCTATTAATCTAC+CGG | - | chr3.2:30437130-30437149 | MS.gene51013:intron | 35.0% | |
| GAGCTTAGTTCATTTGGTAA+GGG | - | chr3.2:30437269-30437288 | MS.gene51013:intron | 35.0% | |
| TATATCAGGTTTCAGCTTCA+AGG | - | chr3.2:30437676-30437695 | MS.gene51013:intron | 35.0% | |
| TCTTGTAGATGGGAAAAAAC+TGG | + | chr3.2:30437577-30437596 | None:intergenic | 35.0% | |
| TGAGCTTAGTTCATTTGGTA+AGG | - | chr3.2:30437268-30437287 | MS.gene51013:intron | 35.0% | |
| TTAATCCCCTCGTATTTGTT+AGG | + | chr3.2:30437538-30437557 | None:intergenic | 35.0% | |
| ! | TTTCTTGGTCAATTAGCCTA+GGG | + | chr3.2:30437369-30437388 | None:intergenic | 35.0% |
| !! | TTATTCACCTTTAAGGTCTC+TGG | - | chr3.2:30437342-30437361 | MS.gene51013:intron | 35.0% |
| !! | TTTTCTTGGTCAATTAGCCT+AGG | + | chr3.2:30437370-30437389 | None:intergenic | 35.0% |
| AAGATCATGGATCTGATCGT+CGG | + | chr3.2:30436766-30436785 | None:intergenic | 40.0% | |
| AGCTCAGAAGGACTTCTTTA+CGG | + | chr3.2:30437254-30437273 | None:intergenic | 40.0% | |
| AGGTGAATAAGTATGGTGTC+TGG | + | chr3.2:30437332-30437351 | None:intergenic | 40.0% | |
| ATAGGCCTAACAAATACGAG+GGG | - | chr3.2:30437530-30437549 | MS.gene51013:intron | 40.0% | |
| CAATAGGCCTAACAAATACG+AGG | - | chr3.2:30437528-30437547 | MS.gene51013:intron | 40.0% | |
| CCAAATGAACTAAGCTCAGA+AGG | + | chr3.2:30437266-30437285 | None:intergenic | 40.0% | |
| CCAGGGACATAACAAGAAAA+AGG | - | chr3.2:30437500-30437519 | MS.gene51013:intron | 40.0% | |
| CCTTCTGAGCTTAGTTCATT+TGG | - | chr3.2:30437263-30437282 | MS.gene51013:intron | 40.0% | |
| GACTCTCCGGTAGATTAATA+GGG | + | chr3.2:30437139-30437158 | None:intergenic | 40.0% | |
| GGTGAATAAGTATGGTGTCT+GGG | + | chr3.2:30437331-30437350 | None:intergenic | 40.0% | |
| GTGAATAAGTATGGTGTCTG+GGG | + | chr3.2:30437330-30437349 | None:intergenic | 40.0% | |
| TGACTCTCCGGTAGATTAAT+AGG | + | chr3.2:30437140-30437159 | None:intergenic | 40.0% | |
| TGCATAATATATGCAGGGAC+CGG | - | chr3.2:30437296-30437315 | MS.gene51013:intron | 40.0% | |
| TTCTTCCTGAGATTCGCAAT+AGG | - | chr3.2:30436805-30436824 | MS.gene51013:CDS | 40.0% | |
| ! | TTCTTGGTCAATTAGCCTAG+GGG | + | chr3.2:30437368-30437387 | None:intergenic | 40.0% |
| !! | CCTTTTTCTTGTTATGTCCC+TGG | + | chr3.2:30437503-30437522 | None:intergenic | 40.0% |
| !! | GAGCTGTTTTGTGAGCTTTT+AGG | + | chr3.2:30437182-30437201 | None:intergenic | 40.0% |
| ACCAACTTAATCCTAGGTCC+AGG | - | chr3.2:30437482-30437501 | MS.gene51013:intron | 45.0% | |
| AGGAAGAAGTTGCTGCAACT+TGG | + | chr3.2:30436793-30436812 | None:intergenic | 45.0% | |
| CCAACTTAATCCTAGGTCCA+GGG | - | chr3.2:30437483-30437502 | MS.gene51013:intron | 45.0% | |
| GCAACTTGGAGACAAGATCA+TGG | + | chr3.2:30436779-30436798 | None:intergenic | 45.0% | |
| TGAGCGTTTGTCTGAGCTAT+TGG | - | chr3.2:30437758-30437777 | MS.gene51013:CDS | 45.0% | |
| CACGGCAAACAAGTAGTTCG+AGG | - | chr3.2:30436733-30436752 | MS.gene51013:CDS | 50.0% | |
| CCCTGGACCTAGGATTAAGT+TGG | + | chr3.2:30437486-30437505 | None:intergenic | 50.0% | |
| GAGCTCCTATTGCGAATCTC+AGG | + | chr3.2:30436813-30436832 | None:intergenic | 50.0% | |
| GCTCAGACAAACGCTCACTT+AGG | + | chr3.2:30437755-30437774 | None:intergenic | 50.0% | |
| ! | AGAGTCAGCTCTAACCATGG+TGG | - | chr3.2:30437153-30437172 | MS.gene51013:intron | 50.0% |
| ! | CATGGTTAGAGCTGACTCTC+CGG | + | chr3.2:30437152-30437171 | None:intergenic | 50.0% |
| ! | CTTGTTATGTCCCTGGACCT+AGG | + | chr3.2:30437496-30437515 | None:intergenic | 50.0% |
| ! | TCGCAATAGGAGCTCTGACA+AGG | - | chr3.2:30436818-30436837 | MS.gene51013:CDS | 50.0% |
| !! | GAGCTTTTAGGAGTCCACCA+TGG | + | chr3.2:30437170-30437189 | None:intergenic | 50.0% |
| !! | TTCAGCTTCAAGGGTGTTGC+AGG | - | chr3.2:30437686-30437705 | MS.gene51013:intron | 50.0% |
| CTAGGGGCCAGAGACCTTAA+AGG | + | chr3.2:30437352-30437371 | None:intergenic | 55.0% | |
| ! | CGGAGAGTCAGCTCTAACCA+TGG | - | chr3.2:30437150-30437169 | MS.gene51013:intron | 55.0% |
| !! | CTTTAAGGTCTCTGGCCCCT+AGG | - | chr3.2:30437350-30437369 | MS.gene51013:intron | 55.0% |
| ! | GGTGTCTGGGGTTCGAACTC+CGG | + | chr3.2:30437318-30437337 | None:intergenic | 60.0% |
| CTAGCAGGAGGTCGCGGTCA+CGG | - | chr3.2:30436715-30436734 | MS.gene51013:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 30436711 | 30437833 | 30436711 | ID=MS.gene51013 |
| chr3.2 | mRNA | 30436711 | 30437833 | 30436711 | ID=MS.gene51013.t1;Parent=MS.gene51013 |
| chr3.2 | exon | 30437705 | 30437833 | 30437705 | ID=MS.gene51013.t1.exon1;Parent=MS.gene51013.t1 |
| chr3.2 | CDS | 30437705 | 30437833 | 30437705 | ID=cds.MS.gene51013.t1;Parent=MS.gene51013.t1 |
| chr3.2 | exon | 30436711 | 30436860 | 30436711 | ID=MS.gene51013.t1.exon2;Parent=MS.gene51013.t1 |
| chr3.2 | CDS | 30436711 | 30436860 | 30436711 | ID=cds.MS.gene51013.t1;Parent=MS.gene51013.t1 |
| Gene Sequence |
| Protein sequence |
