AlfalfaGEDB | MS.gene51026

M. sativa cultivar XinJiangDaYe / MS.gene51026

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51026.t1	KEH33404.1	82.8	180	25	3	1	174	7	186	1.10E-72	282.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51026.t1	A0A072UUA3	82.8	180	25	3	1	174	7	186	8.2e-73	282.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene51026.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51026.t1	MTR_3g437790	82.778	180	25	3	1	174	7	186	2.04e-96	277
MS.gene51026.t1	MTR_2g045020	64.072	167	52	6	6	168	19	181	3.03e-56	175
MS.gene51026.t1	MTR_8g022970	50.857	175	73	6	2	167	10	180	1.59e-46	150
MS.gene51026.t1	MTR_3g109930	51.656	151	69	3	18	166	16	164	8.30e-45	145
MS.gene51026.t1	MTR_2g105290	36.025	161	95	3	1	155	3	161	1.05e-23	92.0
MS.gene51026.t1	MTR_7g028448	38.060	134	71	3	23	155	31	153	7.44e-22	87.0
MS.gene51026.t1	MTR_4g036915	38.060	134	71	3	23	155	31	153	7.44e-22	87.0
MS.gene51026.t1	MTR_8g086290	35.065	154	92	3	5	152	7	158	1.04e-20	84.3
MS.gene51026.t1	MTR_5g053390	36.774	155	86	4	8	152	7	159	1.88e-18	79.7
MS.gene51026.t1	MTR_4g127140	33.803	142	84	3	23	155	31	171	4.39e-17	75.1
MS.gene51026.t1	MTR_3g052920	32.911	158	88	3	8	152	7	159	2.29e-16	74.3
MS.gene51026.t1	MTR_8g043650	30.909	165	95	4	1	152	1	159	4.10e-16	73.6
MS.gene51026.t1	MTR_1g012570	32.298	161	85	5	8	152	7	159	1.04e-15	72.4
MS.gene51026.t1	MTR_1g106070	30.909	165	95	4	1	152	1	159	1.25e-15	71.2
MS.gene51026.t1	MTR_1g105920	31.646	158	90	4	8	152	7	159	1.38e-15	72.0
MS.gene51026.t1	MTR_3g030770	33.125	160	83	5	9	152	8	159	2.03e-15	71.6
MS.gene51026.t1	MTR_3g080940	29.861	144	83	3	23	156	28	163	4.68e-15	70.1
MS.gene51026.t1	MTR_1g105910	31.013	158	91	3	8	152	7	159	6.02e-15	70.5
MS.gene51026.t1	MTR_2g085250	29.375	160	91	4	8	152	7	159	7.86e-15	70.1
MS.gene51026.t1	MTR_2g085280	29.375	160	91	4	8	152	7	159	8.89e-15	70.1
MS.gene51026.t1	MTR_1g108510	32.500	160	84	5	9	152	8	159	1.77e-14	68.9
MS.gene51026.t1	MTR_2g030740	32.298	161	85	5	8	152	7	159	2.04e-14	68.9
MS.gene51026.t1	MTR_1g108580	32.500	160	84	5	9	152	8	159	6.12e-14	67.4
MS.gene51026.t1	MTR_8g046350	42.466	73	36	1	11	77	3	75	1.56e-13	65.1
MS.gene51026.t1	MTR_5g055100	32.895	152	76	6	8	142	7	149	2.39e-13	65.9
MS.gene51026.t1	MTR_0121s0080	33.824	136	78	4	24	152	29	159	5.58e-12	62.4
MS.gene51026.t1	MTR_1g047550	34.615	104	60	2	11	106	3	106	7.42e-12	61.2
MS.gene51026.t1	MTR_8g051580	46.552	58	31	0	23	80	21	78	7.82e-12	60.8
MS.gene51026.t1	MTR_3g052870	43.056	72	35	1	8	73	7	78	7.84e-12	61.6
MS.gene51026.t1	MTR_1g105905	30.216	139	79	4	23	151	11	141	9.84e-12	61.2
MS.gene51026.t1	MTR_2g093190	30.573	157	91	4	9	152	8	159	1.57e-11	60.8
MS.gene51026.t1	MTR_8g079502	30.631	111	69	2	11	113	3	113	1.88e-11	60.1
MS.gene51026.t1	MTR_1g077360	29.193	161	90	3	8	152	7	159	2.50e-11	60.5
MS.gene51026.t1	MTR_7g062350	33.577	137	79	4	23	152	40	171	4.90e-11	59.7
MS.gene51026.t1	MTR_1g063160	42.647	68	33	1	11	72	3	70	8.24e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51026.t1	AT5G60440	31.081	148	93	4	23	164	26	170	1.48e-16	75.5
MS.gene51026.t1	AT2G24840	29.299	157	101	4	16	162	72	228	5.43e-16	73.6
MS.gene51026.t1	AT3G66656	51.020	49	24	0	23	71	21	69	2.75e-12	62.4
MS.gene51026.t1	AT4G36590	28.571	161	97	5	8	152	5	163	6.09e-12	62.4
MS.gene51026.t1	AT2G34440	29.508	122	80	2	23	141	21	139	9.14e-12	60.8
MS.gene51026.t1	AT1G65360	29.655	145	83	5	8	139	5	143	2.83e-11	60.5

Find 26 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACACACATTCTCTTGAAT+TGG	0.284587	3.2:+30718152	None:intergenic
GAATAAATCTATTGATGATA+TGG	0.314688	3.2:-30718267	MS.gene51026:CDS
TGAATGACAGCATCATAGTT+AGG	0.369392	3.2:+30718470	None:intergenic
TCGCTTCATATCGAAGCCTT+AGG	0.376463	3.2:+30718378	None:intergenic
TTGTTTCTCTCGGCCGCCAT+TGG	0.404729	3.2:+30718656	None:intergenic
CTTCCGTGTGTTGTTTCTCT+CGG	0.413838	3.2:+30718646	None:intergenic
CCCTTCTCGAAACTCAAACT+TGG	0.418484	3.2:-30718590	MS.gene51026:CDS
CGATGAGTGCAGTTTCTGAT+TGG	0.423120	3.2:+30718531	None:intergenic
CGCTTCATATCGAAGCCTTA+GGG	0.430275	3.2:+30718379	None:intergenic
CTCATCCTCGTCCATATGTC+CGG	0.445428	3.2:+30718436	None:intergenic
CTTCTTCGCTTCGCTTGCTA+CGG	0.451335	3.2:+30718187	None:intergenic
GAAGGATGAAATCAAGAAAG+AGG	0.477445	3.2:-30718618	MS.gene51026:CDS
GATTTATTCCACCAATCAGC+AGG	0.485193	3.2:+30718281	None:intergenic
CTTCGATATGAAGCGATTCA+AGG	0.502715	3.2:-30718371	MS.gene51026:CDS
GAATGACAGCATCATAGTTA+GGG	0.520135	3.2:+30718471	None:intergenic
CATGCATAGAACTTGTTGTT+GGG	0.522753	3.2:+30718500	None:intergenic
AGGAAAACTTAAAGAAGAGA+AGG	0.524858	3.2:-30718351	MS.gene51026:CDS
CAACACACGGAAGAGAAAGA+AGG	0.527351	3.2:-30718636	MS.gene51026:CDS
CCAAGTTTGAGTTTCGAGAA+GGG	0.530556	3.2:+30718590	None:intergenic
TTGATTTGCCTGCTGATTGG+TGG	0.538450	3.2:-30718289	MS.gene51026:CDS
TCCAAGTTTGAGTTTCGAGA+AGG	0.565563	3.2:+30718589	None:intergenic
ATGATGTTGTTGAAACCCTA+AGG	0.589934	3.2:-30718394	MS.gene51026:CDS
ACATGCATAGAACTTGTTGT+TGG	0.594378	3.2:+30718499	None:intergenic
AACAACAAGTTCTATGCATG+TGG	0.619639	3.2:-30718497	MS.gene51026:CDS
CGGCCGAGAGAAACAACACA+CGG	0.685523	3.2:-30718649	MS.gene51026:CDS
TCTGACCGGACATATGGACG+AGG	0.696130	3.2:-30718441	MS.gene51026:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GAATAAATCTATTGATGATA+TGG	-	chr3.2:30718537-30718556	MS.gene51026:CDS	20.0%
	AGGAAAACTTAAAGAAGAGA+AGG	-	chr3.2:30718453-30718472	MS.gene51026:CDS	30.0%
	TAACACACATTCTCTTGAAT+TGG	+	chr3.2:30718655-30718674	None:intergenic	30.0%
	AACAACAAGTTCTATGCATG+TGG	-	chr3.2:30718307-30718326	MS.gene51026:CDS	35.0%
	ATGATGTTGTTGAAACCCTA+AGG	-	chr3.2:30718410-30718429	MS.gene51026:CDS	35.0%
	GAAGGATGAAATCAAGAAAG+AGG	-	chr3.2:30718186-30718205	MS.gene51026:CDS	35.0%
	GAATGACAGCATCATAGTTA+GGG	+	chr3.2:30718336-30718355	None:intergenic	35.0%
	TGAATGACAGCATCATAGTT+AGG	+	chr3.2:30718337-30718356	None:intergenic	35.0%
!!	ACATGCATAGAACTTGTTGT+TGG	+	chr3.2:30718308-30718327	None:intergenic	35.0%
!!	CATGCATAGAACTTGTTGTT+GGG	+	chr3.2:30718307-30718326	None:intergenic	35.0%
!!!	ATTTTGATTTGCCTGCTGAT+TGG	-	chr3.2:30718512-30718531	MS.gene51026:CDS	35.0%
	CTTCGATATGAAGCGATTCA+AGG	-	chr3.2:30718433-30718452	MS.gene51026:CDS	40.0%
	GATTTATTCCACCAATCAGC+AGG	+	chr3.2:30718526-30718545	None:intergenic	40.0%
!	CCAAGTTTGAGTTTCGAGAA+GGG	+	chr3.2:30718217-30718236	None:intergenic	40.0%
!	TCCAAGTTTGAGTTTCGAGA+AGG	+	chr3.2:30718218-30718237	None:intergenic	40.0%
	CAACACACGGAAGAGAAAGA+AGG	-	chr3.2:30718168-30718187	MS.gene51026:CDS	45.0%
	CCCTTCTCGAAACTCAAACT+TGG	-	chr3.2:30718214-30718233	MS.gene51026:CDS	45.0%
	CGATGAGTGCAGTTTCTGAT+TGG	+	chr3.2:30718276-30718295	None:intergenic	45.0%
	CGCTTCATATCGAAGCCTTA+GGG	+	chr3.2:30718428-30718447	None:intergenic	45.0%
	TCGCTTCATATCGAAGCCTT+AGG	+	chr3.2:30718429-30718448	None:intergenic	45.0%
!	GTCATTCAGCGTTTTCTGAC+CGG	-	chr3.2:30718349-30718368	MS.gene51026:CDS	45.0%
!!	CTTCCGTGTGTTGTTTCTCT+CGG	+	chr3.2:30718161-30718180	None:intergenic	45.0%
!!	TTGATTTGCCTGCTGATTGG+TGG	-	chr3.2:30718515-30718534	MS.gene51026:CDS	45.0%
	CTCATCCTCGTCCATATGTC+CGG	+	chr3.2:30718371-30718390	None:intergenic	50.0%
!	CTTCTTCGCTTCGCTTGCTA+CGG	+	chr3.2:30718620-30718639	None:intergenic	50.0%
!	GCGTTTTCTGACCGGACATA+TGG	-	chr3.2:30718357-30718376	MS.gene51026:CDS	50.0%
	CGGCCGAGAGAAACAACACA+CGG	-	chr3.2:30718155-30718174	MS.gene51026:CDS	55.0%
	TCTGACCGGACATATGGACG+AGG	-	chr3.2:30718363-30718382	MS.gene51026:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	30718151	30718675	30718151	ID=MS.gene51026
chr3.2	mRNA	30718151	30718675	30718151	ID=MS.gene51026.t1;Parent=MS.gene51026
chr3.2	exon	30718151	30718675	30718151	ID=MS.gene51026.t1.exon1;Parent=MS.gene51026.t1
chr3.2	CDS	30718151	30718675	30718151	ID=cds.MS.gene51026.t1;Parent=MS.gene51026.t1

Gene Sequence

>MS.gene51026

ATGGCGGCCGAGAGAAACAACACACGGAAGAGAAAGAAGGATGAAATCAAGAAAGAGGAAAAGCCCTTCTCGAAACTCAAACTTGGAATTTTCAACAAAGCAATAGAGCTATCTATTCTATGCCAATCAGAAACTGCACTCATCGTAACATCACCCAACAACAAGTTCTATGCATGTGGCTACCCTAACTATGATGCTGTCATTCAGCGTTTTCTGACCGGACATATGGACGAGGATGAGAAAAATAAGCAAGAAGATGATGATGTTGTTGAAACCCTAAGGCTTCGATATGAAGCGATTCAAGGAAAACTTAAAGAAGAGAAGGAGAAATTGCAAGCTGTCAAAGATGCACAAAAGAGTAATTTTGATTTGCCTGCTGATTGGTGGAATAAATCTATTGATGATATGGATTTAACATCTCTTGAAGATATTACGACTTCGTTGAATAAATTGAAGATTAATTTGTCCGTAGCAAGCGAAGCGAAGAAGTTCAATTTACATCCAATTCAAGAGAATGTGTGTTAA

Protein sequence

>MS.gene51026.t1

MAAERNNTRKRKKDEIKKEEKPFSKLKLGIFNKAIELSILCQSETALIVTSPNNKFYACGYPNYDAVIQRFLTGHMDEDEKNKQEDDDVVETLRLRYEAIQGKLKEEKEKLQAVKDAQKSNFDLPADWWNKSIDDMDLTSLEDITTSLNKLKINLSVASEAKKFNLHPIQENVC