AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51125

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51125.t1 MTR_5g083960 97.449 196 5 0 1 196 1 196 1.89e-144 400
MS.gene51125.t1 MTR_3g094690 63.025 238 43 8 1 195 1 236 7.79e-84 248
MS.gene51125.t1 MTR_3g094690 62.605 238 43 9 1 195 1 235 2.82e-81 241
MS.gene51125.t1 MTR_5g075020 59.052 232 55 7 1 195 1 229 2.49e-71 216
MS.gene51125.t1 MTR_5g075020 58.929 224 53 6 9 195 12 233 6.26e-69 210
MS.gene51125.t1 MTR_5g083230 60.504 119 47 0 34 152 1 119 7.79e-49 157
MS.gene51125.t1 MTR_2g068760 63.063 111 41 0 36 146 10 120 6.54e-48 154
MS.gene51125.t1 MTR_3g071590 67.619 105 34 0 35 139 4 108 7.45e-48 154
MS.gene51125.t1 MTR_4g083680 62.727 110 41 0 29 138 5 114 2.14e-47 153
MS.gene51125.t1 MTR_4g105170 62.385 109 41 0 38 146 10 118 1.19e-44 147
MS.gene51125.t1 MTR_7g033800 62.617 107 40 0 36 142 8 114 1.99e-44 145
MS.gene51125.t1 MTR_2g093310 55.752 113 50 0 38 150 32 144 9.25e-44 145
MS.gene51125.t1 MTR_3g031660 55.645 124 50 1 37 155 5 128 1.46e-43 144
MS.gene51125.t1 MTR_3g077240 59.091 110 45 0 37 146 8 117 8.36e-43 142
MS.gene51125.t1 MTR_6g005070 60.577 104 41 0 36 139 6 109 1.15e-42 140
MS.gene51125.t1 MTR_3g073690 59.813 107 43 0 36 142 6 112 1.72e-42 142
MS.gene51125.t1 MTR_5g017950 60.748 107 42 0 36 142 5 111 3.12e-42 144
MS.gene51125.t1 MTR_4g060950 55.263 114 50 1 38 150 23 136 6.26e-42 140
MS.gene51125.t1 MTR_6g005080 57.009 107 46 0 36 142 7 113 3.52e-41 136
MS.gene51125.t1 MTR_3g071420 59.804 102 41 0 36 137 11 112 1.96e-40 136
MS.gene51125.t1 MTR_3g452660 55.963 109 48 0 36 144 4 112 7.97e-40 137
MS.gene51125.t1 MTR_1g095850 54.955 111 50 0 40 150 73 183 8.72e-40 135
MS.gene51125.t1 MTR_7g074990 62.745 102 37 1 39 139 20 121 1.31e-39 135
MS.gene51125.t1 MTR_8g040900 43.590 156 88 0 36 191 4 159 1.07e-38 132
MS.gene51125.t1 MTR_5g083010 55.660 106 47 0 36 141 8 113 1.15e-38 131
MS.gene51125.t1 MTR_7g096530 53.333 105 48 1 36 139 11 115 2.09e-36 126
MS.gene51125.t1 MTR_7g028905 53.333 105 49 0 40 144 6 110 5.91e-36 122
MS.gene51125.t1 MTR_8g036085 47.794 136 65 3 39 168 23 158 5.83e-35 123
MS.gene51125.t1 MTR_5g093010 46.457 127 65 1 40 166 6 129 2.99e-34 119
MS.gene51125.t1 MTR_8g036105 50.435 115 56 1 39 152 18 132 4.54e-34 120
MS.gene51125.t1 MTR_1g070220 53.465 101 46 1 39 138 15 115 5.83e-34 120
MS.gene51125.t1 MTR_6g027710 49.107 112 56 1 38 148 39 150 2.27e-33 119
MS.gene51125.t1 MTR_7g096610 53.398 103 45 2 38 138 7 108 2.80e-32 116
MS.gene51125.t1 MTR_1g070205 53.922 102 44 2 39 138 12 112 4.42e-32 116
MS.gene51125.t1 MTR_5g080470 50.505 99 48 1 38 135 6 104 3.83e-30 109
MS.gene51125.t1 MTR_8g079660 47.115 104 55 0 39 142 8 111 1.62e-29 110
MS.gene51125.t1 MTR_7g075230 42.405 158 79 5 39 191 12 162 1.78e-28 105
MS.gene51125.t1 MTR_6g027700 42.718 103 58 1 37 138 5 107 3.27e-27 102
MS.gene51125.t1 MTR_8g079620 43.269 104 59 0 39 142 8 111 4.83e-26 100
MS.gene51125.t1 MTR_6g011250 39.806 103 61 1 32 133 2 104 3.72e-25 96.7
MS.gene51125.t1 MTR_8g079580 46.667 105 55 1 39 142 8 112 3.72e-25 97.8
MS.gene51125.t1 MTR_4g107450 37.705 122 76 0 36 157 7 128 2.36e-24 97.4
MS.gene51125.t1 MTR_6g011230 38.235 102 63 0 35 136 7 108 1.39e-23 93.2
MS.gene51125.t1 MTR_6g011200 37.755 98 60 1 40 136 10 107 8.92e-22 89.7
MS.gene51125.t1 MTR_2g100020 39.048 105 62 2 36 139 16 119 8.43e-21 87.4
MS.gene51125.t1 MTR_8g017090 35.780 109 68 2 36 143 9 116 3.95e-20 86.3
MS.gene51125.t1 MTR_5g085390 34.579 107 66 2 34 136 1 107 1.71e-19 83.2
MS.gene51125.t1 MTR_4g099230 35.714 98 62 1 40 136 3 100 3.10e-19 80.5
MS.gene51125.t1 MTR_4g088035 32.955 88 59 0 38 125 5 92 7.16e-14 68.6
MS.gene51125.t1 MTR_8g009730 27.891 147 99 2 40 185 11 151 2.53e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51125.t1 AT2G40470 73.054 167 34 4 1 157 1 166 1.66e-71 216
MS.gene51125.t1 AT2G40470 79.851 134 24 1 27 157 8 141 4.33e-67 204
MS.gene51125.t1 AT2G30340 62.424 165 49 2 1 152 1 165 4.43e-67 206
MS.gene51125.t1 AT2G30340 74.797 123 31 0 30 152 44 166 4.13e-65 201
MS.gene51125.t1 AT2G30130 66.990 103 34 0 37 139 6 108 7.07e-46 150
MS.gene51125.t1 AT1G31320 60.345 116 46 0 36 151 10 125 8.35e-46 149
MS.gene51125.t1 AT5G63090 53.285 137 61 1 37 170 9 145 2.21e-43 143
MS.gene51125.t1 AT5G63090 53.285 137 61 1 37 170 9 145 2.21e-43 143
MS.gene51125.t1 AT5G63090 53.285 137 61 1 37 170 9 145 2.21e-43 143
MS.gene51125.t1 AT5G63090 53.285 137 61 1 37 170 9 145 2.21e-43 143
MS.gene51125.t1 AT3G27650 60.748 107 42 0 36 142 36 142 2.86e-43 142
MS.gene51125.t1 AT5G66870 55.072 138 57 3 36 173 4 136 9.26e-43 145
MS.gene51125.t1 AT1G07900 54.867 113 51 0 38 150 32 144 4.79e-42 140
MS.gene51125.t1 AT1G16530 50.704 142 63 3 23 162 3 139 7.75e-42 139
MS.gene51125.t1 AT2G28500 54.867 113 51 0 38 150 54 166 1.75e-41 140
MS.gene51125.t1 AT2G23660 57.944 107 45 0 36 142 2 108 6.74e-41 140
MS.gene51125.t1 AT2G23660 57.944 107 45 0 36 142 2 108 6.74e-41 140
MS.gene51125.t1 AT2G23660 57.944 107 45 0 36 142 2 108 6.74e-41 140
MS.gene51125.t1 AT1G65620 61.468 109 42 0 35 143 5 113 1.53e-40 137
MS.gene51125.t1 AT1G65620 61.468 109 42 0 35 143 5 113 1.53e-40 137
MS.gene51125.t1 AT1G65620 61.468 109 42 0 35 143 5 113 1.53e-40 137
MS.gene51125.t1 AT1G65620 61.468 109 42 0 35 143 5 113 1.53e-40 137
MS.gene51125.t1 AT1G65620 61.468 109 42 0 35 143 5 113 1.53e-40 137
MS.gene51125.t1 AT3G11090 52.500 120 57 0 36 155 8 127 4.61e-38 129
MS.gene51125.t1 AT3G26620 55.000 100 45 0 40 139 6 105 4.32e-37 125
MS.gene51125.t1 AT3G26660 55.000 100 45 0 40 139 6 105 5.03e-37 125
MS.gene51125.t1 AT2G42430 47.656 128 66 1 29 155 5 132 6.71e-36 126
MS.gene51125.t1 AT3G03760 51.786 112 53 1 38 148 50 161 5.81e-35 124
MS.gene51125.t1 AT4G00210 53.922 102 46 1 39 139 11 112 1.02e-33 120
MS.gene51125.t1 AT2G31310 51.961 102 48 1 38 138 6 107 9.04e-33 116
MS.gene51125.t1 AT4G00220 55.769 104 45 1 42 144 20 123 1.37e-32 117
MS.gene51125.t1 AT3G58190 52.941 102 45 2 39 138 11 111 1.83e-32 116
MS.gene51125.t1 AT5G06080 49.107 112 56 1 38 148 6 117 5.26e-32 114
MS.gene51125.t1 AT2G45420 50.962 104 50 1 42 144 40 143 8.03e-31 113
MS.gene51125.t1 AT2G42440 50.485 103 48 2 38 138 6 107 1.69e-30 112
MS.gene51125.t1 AT2G45410 51.515 99 47 1 42 139 19 117 1.25e-29 108
MS.gene51125.t1 AT3G50510 41.026 117 69 0 37 153 10 126 1.41e-26 100
MS.gene51125.t1 AT3G50510 41.026 117 69 0 37 153 10 126 1.41e-26 100
MS.gene51125.t1 AT3G47870 38.889 108 66 0 36 143 33 140 1.17e-25 101
MS.gene51125.t1 AT1G72980 40.385 104 62 0 36 139 10 113 1.46e-22 90.9
MS.gene51125.t1 AT5G35900 37.500 104 65 0 36 139 2 105 5.17e-22 89.4
MS.gene51125.t1 AT3G13850 33.654 104 69 0 40 143 37 140 8.05e-20 84.7
MS.gene51125.t1 AT1G06280 46.753 77 41 0 40 116 25 101 1.38e-18 80.1
MS.gene51125.t1 AT4G22700 39.216 102 51 4 40 132 6 105 1.27e-15 72.0
MS.gene51125.t1 AT2G19820 33.333 90 59 1 38 126 11 100 4.33e-15 68.9
MS.gene51125.t1 AT5G15060 38.202 89 49 3 27 110 3 90 8.43e-12 60.8
MS.gene51125.t1 AT1G36000 30.108 93 63 2 34 125 4 95 2.04e-11 59.3

Find 34 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGAGAAATATGGAGAAAA+AGG 0.233084 5.1:+66166381 None:intergenic
CTTGCAAATTTAATCAAAAT+AGG 0.235336 5.1:+66165328 None:intergenic
GCTTCTGTCCACAAAGTCTT+TGG 0.267396 5.1:-66166339 MS.gene51125:CDS
TTTCAAAAGGGAAGAATGTT+AGG 0.277293 5.1:-66166480 MS.gene51125:CDS
AGGCTAAGAGATCCAGTTTA+TGG 0.306813 5.1:-66165590 MS.gene51125:CDS
GCATCTGCTCTTTGATTCTC+TGG 0.341032 5.1:+66165644 None:intergenic
GGAAGAATGTTAGGTCCTTC+AGG 0.355019 5.1:-66166471 MS.gene51125:CDS
GGCTCATGAGGAGAGAAATA+TGG 0.368976 5.1:+66166372 None:intergenic
GCACCCATGCATCCATAAAC+TGG 0.381798 5.1:+66165578 None:intergenic
ACTTGAAATTGAGCTATAAC+TGG 0.426496 5.1:+66165361 None:intergenic
CAACAAGTTCAATCATTACT+AGG 0.447623 5.1:-66165539 MS.gene51125:CDS
TGCTGGCACCAAAGACTTTG+TGG 0.448216 5.1:+66166331 None:intergenic
TAGCAACTTTGAGACGTTGC+TGG 0.449895 5.1:+66166314 None:intergenic
CAACGTCTCAAAGTTGCTAT+TGG 0.452802 5.1:-66166310 MS.gene51125:intron
TAGTTTATGAAGCAAATGTA+AGG 0.466219 5.1:-66165610 MS.gene51125:CDS
TTTGAGACGAAGGTGTGCTC+AGG 0.487559 5.1:-66166409 MS.gene51125:CDS
TAAATAGAAGAAGGAAGCCT+TGG 0.522625 5.1:+66165404 None:intergenic
GAGATAGGAAAGAAGAGAGA+GGG 0.526552 5.1:-66166513 MS.gene51125:CDS
AGATCCAGTTTATGGATGCA+TGG 0.526865 5.1:-66165582 MS.gene51125:CDS
ATTGATACATCATCTGAAGA+AGG 0.534416 5.1:+66165455 None:intergenic
GCAAACTTCTGAGGCTCATG+AGG 0.547685 5.1:+66166360 None:intergenic
ATGCTTGAATGTCCAGAGAA+AGG 0.549398 5.1:-66166726 None:intergenic
TGAGATAGGAAAGAAGAGAG+AGG 0.549797 5.1:-66166514 MS.gene51125:CDS
AATTCATAAATACAAACTCA+TGG 0.552933 5.1:-66165492 MS.gene51125:CDS
GAAATTGAGCTATAACTGGT+AGG 0.562061 5.1:+66165365 None:intergenic
TGTTGTGTGTAAATAGAAGA+AGG 0.566644 5.1:+66165395 None:intergenic
GATCCAGTTTATGGATGCAT+GGG 0.578062 5.1:-66165581 MS.gene51125:CDS
TGGACAGAAGCAAACTTCTG+AGG 0.589514 5.1:+66166351 None:intergenic
AAAAGCTTGCATGCAGCACA+AGG 0.594669 5.1:+66166429 None:intergenic
GCATGCAGCACAAGGAGTAG+TGG 0.596320 5.1:+66166437 None:intergenic
GTGGTATTTAGTGTTCCTGA+AGG 0.618012 5.1:+66166456 None:intergenic
CACCTTCAACATCACTACCA+AGG 0.661627 5.1:-66165421 MS.gene51125:CDS
AGCCTTGGTAGTGATGTTGA+AGG 0.670441 5.1:+66165419 None:intergenic
CTTGGTAGTGATGTTGAAGG+TGG 0.715413 5.1:+66165422 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATAAAAAAAATTGAG+AGG + chr5.1:66166167-66166186 None:intergenic 10.0%
!! TTAAAAATTATTCTAATTAG+TGG - chr5.1:66166093-66166112 MS.gene51125:intron 10.0%
!!! AAATTGATAACAAAAAATTT+AGG - chr5.1:66165896-66165915 MS.gene51125:intron 10.0%
!!! AATTGATAACAAAAAATTTA+GGG - chr5.1:66165897-66165916 MS.gene51125:intron 10.0%
!!! ATAAATAATTTGTTATTTAG+AGG + chr5.1:66166276-66166295 None:intergenic 10.0%
!!! TAAATAATTTGTTATTTAGA+GGG + chr5.1:66166275-66166294 None:intergenic 10.0%
!!! TATTTCTACTAATTAATATA+TGG - chr5.1:66165405-66165424 MS.gene51125:CDS 10.0%
!! ATATGCTTTAATATCTATAT+AGG - chr5.1:66165504-66165523 MS.gene51125:CDS 15.0%
!! ATATTTGTAATAATGAACTA+AGG + chr5.1:66165786-66165805 None:intergenic 15.0%
!! TATGCTTTAATATCTATATA+GGG - chr5.1:66165505-66165524 MS.gene51125:CDS 15.0%
!!! AAAATTACAAATTTTGTCAT+AGG + chr5.1:66165383-66165402 None:intergenic 15.0%
!!! AAATAATTTGTTATTTAGAG+GGG + chr5.1:66166274-66166293 None:intergenic 15.0%
!!! AAATTACAAATTTTGTCATA+GGG + chr5.1:66165382-66165401 None:intergenic 15.0%
!!! TTAGACAACATTTTTTTAAT+CGG + chr5.1:66165983-66166002 None:intergenic 15.0%
!! AATTCATAAATACAAACTCA+TGG - chr5.1:66166563-66166582 MS.gene51125:intron 20.0%
!! ATGCTTTAATATCTATATAG+GGG - chr5.1:66165506-66165525 MS.gene51125:CDS 20.0%
!! ATGGATATACATTATATAAG+AGG - chr5.1:66165858-66165877 MS.gene51125:intron 20.0%
!! TATACATTATATAAGAGGTT+TGG - chr5.1:66165863-66165882 MS.gene51125:intron 20.0%
!! TGAAAAAAAAGTAACATTTG+TGG - chr5.1:66166361-66166380 MS.gene51125:CDS 20.0%
!! TTGTAAAAGTCCTAAATTAT+TGG + chr5.1:66165947-66165966 None:intergenic 20.0%
!!! TTGATTTTATTTTAAGTCGA+TGG - chr5.1:66165839-66165858 MS.gene51125:intron 20.0%
! AAAAAGTAACATTTGTGGTA+TGG - chr5.1:66166366-66166385 MS.gene51125:CDS 25.0%
! AAAAGTCCTAAATTATTGGT+CGG + chr5.1:66165943-66165962 None:intergenic 25.0%
! GTATGGTTATGTGTTTAATT+AGG - chr5.1:66166383-66166402 MS.gene51125:CDS 25.0%
! TAGTTTATGAAGCAAATGTA+AGG - chr5.1:66166445-66166464 MS.gene51125:CDS 25.0%
! TGCTTTAATATCTATATAGG+GGG - chr5.1:66165507-66165526 MS.gene51125:CDS 25.0%
!! AAAAGTATGCTTGAAAAGTT+TGG + chr5.1:66165434-66165453 None:intergenic 25.0%
!!! AAATGGATTACTAAACAATG+TGG - chr5.1:66166052-66166071 MS.gene51125:intron 25.0%
!!! AATGGATTACTAAACAATGT+GGG - chr5.1:66166053-66166072 MS.gene51125:intron 25.0%
ACTAAAATTCCCACATAGAT+AGG - chr5.1:66166006-66166025 MS.gene51125:intron 30.0%
ACTTGAAATTGAGCTATAAC+TGG + chr5.1:66166697-66166716 None:intergenic 30.0%
ATATACCAAGTTAACCTCAA+AGG + chr5.1:66166120-66166139 None:intergenic 30.0%
ATATAGTCCTGATCTAATTG+TGG + chr5.1:66166235-66166254 None:intergenic 30.0%
ATTCAAGTAGAATGCACATA+AGG + chr5.1:66166310-66166329 None:intergenic 30.0%
ATTGATACATCATCTGAAGA+AGG + chr5.1:66166603-66166622 None:intergenic 30.0%
CAACAAGTTCAATCATTACT+AGG - chr5.1:66166516-66166535 MS.gene51125:CDS 30.0%
CATATATTGATCAGTTCATG+AGG - chr5.1:66166208-66166227 MS.gene51125:intron 30.0%
CTAAAATTCCCACATAGATA+GGG - chr5.1:66166007-66166026 MS.gene51125:intron 30.0%
CTCATGAACTGATCAATATA+TGG + chr5.1:66166210-66166229 None:intergenic 30.0%
TCTACTTGAATACAAAGCAA+TGG - chr5.1:66166319-66166338 MS.gene51125:CDS 30.0%
TGGTTATGTGTTTAATTAGG+AGG - chr5.1:66166386-66166405 MS.gene51125:CDS 30.0%
TGTTGTGTGTAAATAGAAGA+AGG + chr5.1:66166663-66166682 None:intergenic 30.0%
TTTCAAAAGGGAAGAATGTT+AGG - chr5.1:66165575-66165594 MS.gene51125:CDS 30.0%
! GGTGATGATTCTTTTCAAAA+GGG - chr5.1:66165563-66165582 MS.gene51125:CDS 30.0%
GAAATTGAGCTATAACTGGT+AGG + chr5.1:66166693-66166712 None:intergenic 35.0%
GATTTGCCGACCAATAATTT+AGG - chr5.1:66165934-66165953 MS.gene51125:intron 35.0%
GGAGAGAAATATGGAGAAAA+AGG + chr5.1:66165677-66165696 None:intergenic 35.0%
TAAATAGAAGAAGGAAGCCT+TGG + chr5.1:66166654-66166673 None:intergenic 35.0%
! AGAGTTTTCTTACCTTTCTC+TGG + chr5.1:66165344-66165363 None:intergenic 35.0%
! GGGTGATGATTCTTTTCAAA+AGG - chr5.1:66165562-66165581 MS.gene51125:CDS 35.0%
! TTCTAATTAGTGGACCTTTG+AGG - chr5.1:66166103-66166122 MS.gene51125:intron 35.0%
!!! GGGAGATTTTTTGATGAGAT+AGG - chr5.1:66165527-66165546 MS.gene51125:CDS 35.0%
AGATCCAGTTTATGGATGCA+TGG - chr5.1:66166473-66166492 MS.gene51125:CDS 40.0%
AGGCTAAGAGATCCAGTTTA+TGG - chr5.1:66166465-66166484 MS.gene51125:CDS 40.0%
ATGAGGTCCACAATTAGATC+AGG - chr5.1:66166225-66166244 MS.gene51125:intron 40.0%
CAACGTCTCAAAGTTGCTAT+TGG - chr5.1:66165745-66165764 MS.gene51125:intron 40.0%
GAGATAGGAAAGAAGAGAGA+GGG - chr5.1:66165542-66165561 MS.gene51125:CDS 40.0%
GATCCAGTTTATGGATGCAT+GGG - chr5.1:66166474-66166493 MS.gene51125:CDS 40.0%
GGAGAGTCATAGTTGCTAAA+TGG - chr5.1:66166035-66166054 MS.gene51125:intron 40.0%
GTGGTATTTAGTGTTCCTGA+AGG + chr5.1:66165602-66165621 None:intergenic 40.0%
TCCTTAGACCCTATCTATGT+GGG + chr5.1:66166018-66166037 None:intergenic 40.0%
TGAGATAGGAAAGAAGAGAG+AGG - chr5.1:66165541-66165560 MS.gene51125:CDS 40.0%
AAAAGCTTGCATGCAGCACA+AGG + chr5.1:66165629-66165648 None:intergenic 45.0%
CACCTTCAACATCACTACCA+AGG - chr5.1:66166634-66166653 MS.gene51125:intron 45.0%
CTCCTTAGACCCTATCTATG+TGG + chr5.1:66166019-66166038 None:intergenic 45.0%
GCATCTGCTCTTTGATTCTC+TGG + chr5.1:66166414-66166433 None:intergenic 45.0%
GCTTCTGTCCACAAAGTCTT+TGG - chr5.1:66165716-66165735 MS.gene51125:intron 45.0%
GGAAGAATGTTAGGTCCTTC+AGG - chr5.1:66165584-66165603 MS.gene51125:CDS 45.0%
GGCTCATGAGGAGAGAAATA+TGG + chr5.1:66165686-66165705 None:intergenic 45.0%
TCCCACATAGATAGGGTCTA+AGG - chr5.1:66166014-66166033 MS.gene51125:intron 45.0%
TGGACAGAAGCAAACTTCTG+AGG + chr5.1:66165707-66165726 None:intergenic 45.0%
!! AGCCTTGGTAGTGATGTTGA+AGG + chr5.1:66166639-66166658 None:intergenic 45.0%
!! CTTGGTAGTGATGTTGAAGG+TGG + chr5.1:66166636-66166655 None:intergenic 45.0%
!! GTGGACCTTTGAGGTTAACT+TGG - chr5.1:66166112-66166131 MS.gene51125:intron 45.0%
!! TAGCAACTTTGAGACGTTGC+TGG + chr5.1:66165744-66165763 None:intergenic 45.0%
!!! CATGCAAGCTTTTGAGACGA+AGG - chr5.1:66165636-66165655 MS.gene51125:CDS 45.0%
!!! TAATTAGAATAATTTTTAAA+AGG + chr5.1:66166092-66166111 None:intergenic 5.0%
GCACCCATGCATCCATAAAC+TGG + chr5.1:66166480-66166499 None:intergenic 50.0%
TGCTGGCACCAAAGACTTTG+TGG + chr5.1:66165727-66165746 None:intergenic 50.0%
! TTTGAGACGAAGGTGTGCTC+AGG - chr5.1:66165646-66165665 MS.gene51125:CDS 50.0%
!! GCAAACTTCTGAGGCTCATG+AGG + chr5.1:66165698-66165717 None:intergenic 50.0%
GCATGCAGCACAAGGAGTAG+TGG + chr5.1:66165621-66165640 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 66165337 66166740 66165337 ID=MS.gene51125
chr5.1 mRNA 66165337 66166740 66165337 ID=MS.gene51125.t1;Parent=MS.gene51125
chr5.1 exon 66166727 66166740 66166727 ID=MS.gene51125.t1.exon1;Parent=MS.gene51125.t1
chr5.1 CDS 66166727 66166740 66166727 ID=cds.MS.gene51125.t1;Parent=MS.gene51125.t1
chr5.1 exon 66166311 66166551 66166311 ID=MS.gene51125.t1.exon2;Parent=MS.gene51125.t1
chr5.1 CDS 66166311 66166551 66166311 ID=cds.MS.gene51125.t1;Parent=MS.gene51125.t1
chr5.1 exon 66165337 66165672 66165337 ID=MS.gene51125.t1.exon3;Parent=MS.gene51125.t1
chr5.1 CDS 66165337 66165672 66165337 ID=cds.MS.gene51125.t1;Parent=MS.gene51125.t1
Gene Sequence

>MS.gene51125

ATGTCCAGAGAAAGGTAAGAAAACTCTTTCAAAGTATATATTCCCTATGACAAAATTTGTAATTTTAGTATTTCTACTAATTAATATATGGTAACCAAACTTTTCAAGCATACTTTTTTTACTCTTAAAAAGAATTTTGCATAAATTTGTTAATTACTAAATTTCATATATGCTTTAATATCTATATAGGGGGAGATTTTTTGATGAGATAGGAAAGAAGAGAGAGGGTGATGATTCTTTTCAAAAGGGAAGAATGTTAGGTCCTTCAGGAACACTAAATACCACTACTCCTTGTGCTGCATGCAAGCTTTTGAGACGAAGGTGTGCTCAGGAATGTCCTTTTTCTCCATATTTCTCTCCTCATGAGCCTCAGAAGTTTGCTTCTGTCCACAAAGTCTTTGGTGCCAGCAACGTCTCAAAGTTGCTATTGGTATACACATATAAAACCTTAGTTCATTATTACAAATATTTTAGTATAATTTAGTGATAACTGATTTAGCTGTTGATTTTATTTTAAGTCGATGGATATACATTATATAAGAGGTTTGGTGACTTTTACAAATTGATAACAAAAAATTTAGGGTTGATTTAGAGATCGATTTGCCGACCAATAATTTAGGACTTTTACAAATTGATCTCTAAACCGATTAAAAAAATGTTGTCTAATGCACTAAAATTCCCACATAGATAGGGTCTAAGGAGAGTCATAGTTGCTAAATGGATTACTAAACAATGTGGGTTTTTAATATGAACCTTTTAAAAATTATTCTAATTAGTGGACCTTTGAGGTTAACTTGGTATATATCAAGTTAATATTTTTTTTACTTCCTCTCAATTTTTTTTATATTTTTTTTTTTATAAAACTTAATACCATATATTGATCAGTTCATGAGGTCCACAATTAGATCAGGACTATATTAGAGAATAGATAGATCCCCTCTAAATAACAAATTATTTATATATATCTAAACCTTATGTGCATTCTACTTGAATACAAAGCAATGGTGATTAAATTTTATATGAATGAAAAAAAAGTAACATTTGTGGTATGGTTATGTGTTTAATTAGGAGGTACCAGAGAATCAAAGAGCAGATGCAGCAAATAGTTTAGTTTATGAAGCAAATGTAAGGCTAAGAGATCCAGTTTATGGATGCATGGGTGCAATTTCATCTTTGCAACAACAAGTTCAATCATTACTAGGTGAACTCAATGCTATAAGAGAAGAAATTCATAAATACAAACTCATGGAAGCTAACATGCTTCCTTCTTCAGATGATGTATCAATTGCTACTCTACCACCTTCAACATCACTACCAAGGCTTCCTTCTTCTATTTACACACAACAAACAAATCCTACCAGTTATAGCTCAATTTCAAGTGATCATATTTCCTATTTTGATTAA
Protein sequence

>MS.gene51125.t1

MSRERGRFFDEIGKKREGDDSFQKGRMLGPSGTLNTTTPCAACKLLRRRCAQECPFSPYFSPHEPQKFASVHKVFGASNVSKLLLEVPENQRADAANSLVYEANVRLRDPVYGCMGAISSLQQQVQSLLGELNAIREEIHKYKLMEANMLPSSDDVSIATLPPSTSLPRLPSSIYTQQTNPTSYSSISSDHISYFD