AlfalfaGEDB | MS.gene51226

M. sativa cultivar XinJiangDaYe / MS.gene51226

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51226.t1	RHN47048.1	85.8	162	23	0	1	162	1	162	2.30E-70	275

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51226.t1	Q9FFS3	63.2	57	13	1	105	161	70	118	3.2e-12	73.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51226.t1	G7L6N9	86.3	161	22	0	1	161	1	161	1.6e-70	275.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene51226.t1	TF	WRKY

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51226.t1	MTR_7g079010	95.031	161	8	0	1	161	1	161	1.04e-112	319
MS.gene51226.t1	MTR_8g007270	65.116	43	15	0	119	161	132	174	1.35e-13	66.2
MS.gene51226.t1	MTR_7g028415	84.848	33	5	0	129	161	81	113	1.62e-13	65.1
MS.gene51226.t1	MTR_7g028710	84.848	33	5	0	129	161	81	113	1.62e-13	65.1
MS.gene51226.t1	MTR_3g095080	68.421	38	12	0	127	164	154	191	2.14e-13	65.1
MS.gene51226.t1	MTR_5g029850	69.231	39	12	0	119	157	29	67	1.33e-12	60.5
MS.gene51226.t1	MTR_5g043880	58.929	56	14	1	106	161	98	144	1.87e-12	63.9
MS.gene51226.t1	MTR_4g094588	80.000	35	7	0	127	161	164	198	1.92e-12	64.3
MS.gene51226.t1	MTR_2g033820	80.000	35	7	0	127	161	189	223	2.02e-12	64.3
MS.gene51226.t1	MTR_1g086790	70.000	40	12	0	122	161	139	178	3.99e-12	63.2
MS.gene51226.t1	MTR_5g091390	80.000	35	7	0	127	161	143	177	6.11e-12	62.8
MS.gene51226.t1	MTR_8g092140	69.444	36	10	1	127	161	145	180	1.69e-11	60.8
MS.gene51226.t1	MTR_7g109800	50.000	62	22	1	100	161	113	165	1.86e-11	61.2
MS.gene51226.t1	MTR_3g095080	68.571	35	11	0	127	161	154	188	2.28e-11	60.5
MS.gene51226.t1	MTR_3g095040	58.000	50	20	1	113	161	89	138	2.38e-11	60.1
MS.gene51226.t1	MTR_3g095080	63.158	38	14	0	124	161	127	164	2.59e-11	60.1
MS.gene51226.t1	MTR_8g096730	48.387	62	29	1	100	161	32	90	4.04e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51226.t1	AT5G41570	63.158	57	13	1	105	161	70	118	3.69e-17	75.1
MS.gene51226.t1	AT2G46130	69.565	46	14	0	122	167	11	56	3.77e-17	73.2
MS.gene51226.t1	AT2G46130	75.000	40	10	0	122	161	11	50	6.35e-15	67.4
MS.gene51226.t1	AT1G64000	65.909	44	15	0	118	161	91	134	7.03e-15	69.3
MS.gene51226.t1	AT5G13080	85.294	34	5	0	128	161	54	87	1.42e-14	67.4
MS.gene51226.t1	AT2G03340	38.947	95	54	1	71	161	341	435	2.16e-13	67.4
MS.gene51226.t1	AT1G69310	44.186	86	42	2	82	167	94	173	2.22e-13	66.6
MS.gene51226.t1	AT3G01970	82.353	34	6	0	128	161	52	85	4.28e-13	63.5
MS.gene51226.t1	AT2G44745	60.465	43	17	0	119	161	123	165	4.38e-13	65.1
MS.gene51226.t1	AT1G69310	46.250	80	37	2	82	161	94	167	7.59e-13	65.1
MS.gene51226.t1	AT1G69310	46.250	80	37	2	82	161	94	167	7.59e-13	65.1
MS.gene51226.t1	AT1G69310	46.250	80	37	2	82	161	94	167	7.59e-13	65.1
MS.gene51226.t1	AT4G39410	52.083	48	23	0	114	161	196	243	2.27e-12	63.9
MS.gene51226.t1	AT5G46350	34.899	149	71	4	28	161	66	203	8.74e-12	62.4
MS.gene51226.t1	AT5G49520	82.353	34	6	0	128	161	208	241	1.15e-11	62.4
MS.gene51226.t1	AT4G18170	62.500	48	14	1	118	161	145	192	1.37e-11	61.6
MS.gene51226.t1	AT2G38470	50.746	67	25	1	103	161	316	382	9.08e-11	59.7

Find 43 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTTCATCTTCTTCTAATT+TGG	0.109939	7.3:-28055506	MS.gene51226:CDS
AAGAAGTGTTGATGATATTT+TGG	0.221201	7.3:-28055353	MS.gene51226:CDS
ATTAATGGTGGTGGTGGGTT+TGG	0.274937	7.3:+28055733	None:intergenic
GGTGATGGGATATTCATTAA+TGG	0.303707	7.3:+28055718	None:intergenic
GTGGTGGGTTTGGTGGGTCT+TGG	0.304953	7.3:+28055743	None:intergenic
ACAACAATAGCTTGCTTGTT+GGG	0.309012	7.3:-28055570	MS.gene51226:CDS
GGTTATCGTTGGAGGAAATA+TGG	0.317694	7.3:-28055325	MS.gene51226:CDS
GAAACAAGTTAGAGGAGAAA+AGG	0.356863	7.3:-28055432	MS.gene51226:CDS
TTAGAGGAGAAAAGGGTTAG+AGG	0.362096	7.3:-28055424	MS.gene51226:CDS
TTCTTTACAAAATTACCCTT+TGG	0.380075	7.3:-28055650	MS.gene51226:CDS
AGAAAAGGGAAACAAGTTAG+AGG	0.399768	7.3:-28055440	MS.gene51226:CDS
TCAACTACATGAAGAAGAAA+AGG	0.419090	7.3:-28055455	MS.gene51226:CDS
AAACAAGTTAGAGGAGAAAA+GGG	0.432866	7.3:-28055431	MS.gene51226:CDS
AACAACAATAGCTTGCTTGT+TGG	0.439151	7.3:-28055571	MS.gene51226:CDS
GAATCACAAGATCAAGGTCT+TGG	0.450978	7.3:-28055628	MS.gene51226:CDS
ATGGTGGTGGTGGGTTTGGT+GGG	0.457794	7.3:+28055737	None:intergenic
TGGATGATGGTTATCGTTGG+AGG	0.458848	7.3:-28055333	MS.gene51226:CDS
TAAAGAAGGCGTAAAGAAGA+AGG	0.461538	7.3:+28055666	None:intergenic
ATATTCATTAATGGTGGTGG+TGG	0.463366	7.3:+28055727	None:intergenic
AATGGTGGTGGTGGGTTTGG+TGG	0.469202	7.3:+28055736	None:intergenic
GTAATTCAAAATAATGAAAG+TGG	0.470864	7.3:-28055484	MS.gene51226:CDS
AGGAGAAAAGGGTTAGAGGT+GGG	0.487742	7.3:-28055420	MS.gene51226:CDS
TATTCATTAATGGTGGTGGT+GGG	0.497156	7.3:+28055728	None:intergenic
AAAGAAGGCGTAAAGAAGAA+GGG	0.509237	7.3:+28055667	None:intergenic
CTTGATCTTGTGATTCCAAA+GGG	0.509438	7.3:+28055635	None:intergenic
ACATACATGTAGTATATACC+TGG	0.511478	7.3:+28055273	None:intergenic
CAACTACATGAAGAAGAAAA+GGG	0.514940	7.3:-28055454	MS.gene51226:CDS
CCTTTGGAATCACAAGATCA+AGG	0.519006	7.3:-28055634	MS.gene51226:CDS
CTTGCTTGTTGGGGATGCTA+AGG	0.519688	7.3:-28055560	MS.gene51226:CDS
GAGGAGAAAAGGGTTAGAGG+TGG	0.538687	7.3:-28055421	MS.gene51226:CDS
GGGATATTCATTAATGGTGG+TGG	0.540612	7.3:+28055724	None:intergenic
CAACAATAGCTTGCTTGTTG+GGG	0.541714	7.3:-28055569	MS.gene51226:CDS
AAGAAGGCGTAAAGAAGAAG+GGG	0.548737	7.3:+28055668	None:intergenic
CCTTGATCTTGTGATTCCAA+AGG	0.562491	7.3:+28055634	None:intergenic
CTTGTCTGAAATGCAAACCT+TGG	0.566555	7.3:+28055373	None:intergenic
TCAAGAACAGCAAGTATCCC+AGG	0.569780	7.3:-28055291	MS.gene51226:CDS
AGGTCTTGGTGATATTGAGT+GGG	0.577572	7.3:-28055614	MS.gene51226:CDS
AAGGTCTTGGTGATATTGAG+TGG	0.598654	7.3:-28055615	MS.gene51226:CDS
TAATTCAAAATAATGAAAGT+GGG	0.605922	7.3:-28055483	MS.gene51226:CDS
GATGGGATATTCATTAATGG+TGG	0.606842	7.3:+28055721	None:intergenic
AAAAGACAACAAAAGTGCCA+AGG	0.641958	7.3:-28055390	MS.gene51226:CDS
CATACATGTAGTATATACCT+GGG	0.661629	7.3:+28055274	None:intergenic
GGTCTTGGTGATATTGAGTG+GGG	0.698935	7.3:-28055613	MS.gene51226:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATTCAAAATAATGAAAGT+GGG	-	chr7.3:28055535-28055554	MS.gene51226:CDS	15.0%
!!	GTAATTCAAAATAATGAAAG+TGG	-	chr7.3:28055534-28055553	MS.gene51226:CDS	20.0%
!	TTCTTTACAAAATTACCCTT+TGG	-	chr7.3:28055368-28055387	MS.gene51226:CDS	25.0%
!!	AAAGGGTAATTTTGTAAAGA+AGG	+	chr7.3:28055369-28055388	None:intergenic	25.0%
!!!	AAGAAGTGTTGATGATATTT+TGG	-	chr7.3:28055665-28055684	MS.gene51226:CDS	25.0%
	AAACAAGTTAGAGGAGAAAA+GGG	-	chr7.3:28055587-28055606	MS.gene51226:CDS	30.0%
	ACATACATGTAGTATATACC+TGG	+	chr7.3:28055748-28055767	None:intergenic	30.0%
	CAACTACATGAAGAAGAAAA+GGG	-	chr7.3:28055564-28055583	MS.gene51226:CDS	30.0%
	CACTTCATCTTCTTCTAATT+TGG	-	chr7.3:28055512-28055531	MS.gene51226:CDS	30.0%
	CATACATGTAGTATATACCT+GGG	+	chr7.3:28055747-28055766	None:intergenic	30.0%
	TCAACTACATGAAGAAGAAA+AGG	-	chr7.3:28055563-28055582	MS.gene51226:CDS	30.0%
!!!	GTTGATGATATTTTGGATGA+TGG	-	chr7.3:28055672-28055691	MS.gene51226:CDS	30.0%
	AGAAAAGGGAAACAAGTTAG+AGG	-	chr7.3:28055578-28055597	MS.gene51226:CDS	35.0%
	CTTGATCTTGTGATTCCAAA+GGG	+	chr7.3:28055386-28055405	None:intergenic	35.0%
	GAAACAAGTTAGAGGAGAAA+AGG	-	chr7.3:28055586-28055605	MS.gene51226:CDS	35.0%
	GATGGGATATTCATTAATGG+TGG	+	chr7.3:28055300-28055319	None:intergenic	35.0%
!	AAAGAAGGCGTAAAGAAGAA+GGG	+	chr7.3:28055354-28055373	None:intergenic	35.0%
!	TAAAGAAGGCGTAAAGAAGA+AGG	+	chr7.3:28055355-28055374	None:intergenic	35.0%
!!	AAAAGACAACAAAAGTGCCA+AGG	-	chr7.3:28055628-28055647	MS.gene51226:CDS	35.0%
!!	AACAACAATAGCTTGCTTGT+TGG	-	chr7.3:28055447-28055466	MS.gene51226:CDS	35.0%
!!	ACAACAATAGCTTGCTTGTT+GGG	-	chr7.3:28055448-28055467	MS.gene51226:CDS	35.0%
!!	ATATTCATTAATGGTGGTGG+TGG	+	chr7.3:28055294-28055313	None:intergenic	35.0%
!!	GGTGATGGGATATTCATTAA+TGG	+	chr7.3:28055303-28055322	None:intergenic	35.0%
!!	TATTCATTAATGGTGGTGGT+GGG	+	chr7.3:28055293-28055312	None:intergenic	35.0%
!!	TTTTGGATGATGGTTATCGT+TGG	-	chr7.3:28055682-28055701	MS.gene51226:CDS	35.0%
!!!	GTGTTGTTGTTTTGAGTTTG+TGG	+	chr7.3:28055324-28055343	None:intergenic	35.0%
!!!	TGTTTTGAGTTTGTGGTGAT+GGG	+	chr7.3:28055317-28055336	None:intergenic	35.0%
!!!	TTGTTTTGAGTTTGTGGTGA+TGG	+	chr7.3:28055318-28055337	None:intergenic	35.0%
	CCTTGATCTTGTGATTCCAA+AGG	+	chr7.3:28055387-28055406	None:intergenic	40.0%
	GAATCACAAGATCAAGGTCT+TGG	-	chr7.3:28055390-28055409	MS.gene51226:CDS	40.0%
	GGGATATTCATTAATGGTGG+TGG	+	chr7.3:28055297-28055316	None:intergenic	40.0%
	GGTTATCGTTGGAGGAAATA+TGG	-	chr7.3:28055693-28055712	MS.gene51226:CDS	40.0%
	TTAGAGGAGAAAAGGGTTAG+AGG	-	chr7.3:28055594-28055613	MS.gene51226:CDS	40.0%
!	AAGAAGGCGTAAAGAAGAAG+GGG	+	chr7.3:28055353-28055372	None:intergenic	40.0%
!	AAGGTCTTGGTGATATTGAG+TGG	-	chr7.3:28055403-28055422	MS.gene51226:CDS	40.0%
!	AGGTCTTGGTGATATTGAGT+GGG	-	chr7.3:28055404-28055423	MS.gene51226:CDS	40.0%
!	CTTGTCTGAAATGCAAACCT+TGG	+	chr7.3:28055648-28055667	None:intergenic	40.0%
!!	CAACAATAGCTTGCTTGTTG+GGG	-	chr7.3:28055449-28055468	MS.gene51226:CDS	40.0%
!!	CCTTTGGAATCACAAGATCA+AGG	-	chr7.3:28055384-28055403	MS.gene51226:CDS	40.0%
!!	GAGTGGGGAAACTTTTTCTT+AGG	-	chr7.3:28055420-28055439	MS.gene51226:CDS	40.0%
	AGGAGAAAAGGGTTAGAGGT+GGG	-	chr7.3:28055598-28055617	MS.gene51226:CDS	45.0%
	TCAAGAACAGCAAGTATCCC+AGG	-	chr7.3:28055727-28055746	MS.gene51226:CDS	45.0%
!	GGTCTTGGTGATATTGAGTG+GGG	-	chr7.3:28055405-28055424	MS.gene51226:CDS	45.0%
!	TGGATGATGGTTATCGTTGG+AGG	-	chr7.3:28055685-28055704	MS.gene51226:CDS	45.0%
!!	ATTAATGGTGGTGGTGGGTT+TGG	+	chr7.3:28055288-28055307	None:intergenic	45.0%
	GAGGAGAAAAGGGTTAGAGG+TGG	-	chr7.3:28055597-28055616	MS.gene51226:CDS	50.0%
!	CTTGCTTGTTGGGGATGCTA+AGG	-	chr7.3:28055458-28055477	MS.gene51226:CDS	50.0%
!!	AATGGTGGTGGTGGGTTTGG+TGG	+	chr7.3:28055285-28055304	None:intergenic	55.0%
!!	ATGGTGGTGGTGGGTTTGGT+GGG	+	chr7.3:28055284-28055303	None:intergenic	55.0%
!!	GTGGTGGGTTTGGTGGGTCT+TGG	+	chr7.3:28055278-28055297	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	28055267	28055773	28055267	ID=MS.gene51226
chr7.3	mRNA	28055267	28055773	28055267	ID=MS.gene51226.t1;Parent=MS.gene51226
chr7.3	exon	28055267	28055773	28055267	ID=MS.gene51226.t1.exon1;Parent=MS.gene51226.t1
chr7.3	CDS	28055267	28055773	28055267	ID=cds.MS.gene51226.t1;Parent=MS.gene51226.t1

Gene Sequence

>MS.gene51226

ATGGATAGCCAAGACCCACCAAACCCACCACCACCATTAATGAATATCCCATCACCACAAACTCAAAACAACAACACTTCTCTCCCCTTCTTCTTTACGCCTTCTTTACAAAATTACCCTTTGGAATCACAAGATCAAGGTCTTGGTGATATTGAGTGGGGAAACTTTTTCTTAGGTCAAAACAACAATAGCTTGCTTGTTGGGGATGCTAAGGAGACATTAAAGATTGACAATATTCAACAATGCACTTCATCTTCTTCTAATTTGGTAATTCAAAATAATGAAAGTGGGAGTTATCAACTACATGAAGAAGAAAAGGGAAACAAGTTAGAGGAGAAAAGGGTTAGAGGTGGGAGAGTGAAAAAGACAACAAAAGTGCCAAGGTTTGCATTTCAGACAAGAAGTGTTGATGATATTTTGGATGATGGTTATCGTTGGAGGAAATATGGACAAAAAGCTGTCAAGAACAGCAAGTATCCCAGGTATATACTACATGTATGTTTGTGA

Protein sequence

>MS.gene51226.t1

MDSQDPPNPPPPLMNIPSPQTQNNNTSLPFFFTPSLQNYPLESQDQGLGDIEWGNFFLGQNNNSLLVGDAKETLKIDNIQQCTSSSSNLVIQNNESGSYQLHEEEKGNKLEEKRVRGGRVKKTTKVPRFAFQTRSVDDILDDGYRWRKYGQKAVKNSKYPRYILHVCL