AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51394

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51394.t1 MTR_0402s0040 36.264 273 144 9 45 311 25 273 3.29e-46 166
MS.gene51394.t1 MTR_7g074790 34.286 175 91 4 42 213 6 159 7.10e-25 102
MS.gene51394.t1 MTR_5g039760 35.088 171 82 3 48 215 12 156 6.10e-21 90.5
MS.gene51394.t1 MTR_4g067290 35.714 168 89 6 46 201 56 216 7.32e-21 91.3
MS.gene51394.t1 MTR_4g067290 35.714 168 89 6 46 201 76 236 8.92e-21 91.7
MS.gene51394.t1 MTR_4g067290 35.714 168 89 6 46 201 116 276 1.84e-20 91.7
MS.gene51394.t1 MTR_5g015480 39.264 163 84 7 14 165 19 177 2.05e-20 90.9
MS.gene51394.t1 MTR_4g067290 34.940 166 91 6 46 201 56 214 4.47e-20 89.4
MS.gene51394.t1 MTR_2g070590 44.762 105 54 4 45 146 16 119 5.61e-20 85.5
MS.gene51394.t1 MTR_4g067290 34.940 166 91 6 46 201 74 232 5.87e-20 89.4
MS.gene51394.t1 MTR_4g067290 34.940 166 91 6 46 201 76 234 5.91e-20 89.4
MS.gene51394.t1 MTR_6g017200 44.330 97 50 3 45 138 57 152 1.04e-18 83.2
MS.gene51394.t1 MTR_2g098920 40.367 109 61 3 46 152 80 186 1.11e-17 82.8
MS.gene51394.t1 MTR_2g098880 37.097 124 64 5 35 149 49 167 3.67e-17 81.3
MS.gene51394.t1 MTR_1g057920 43.956 91 47 3 46 133 34 123 3.70e-17 77.8
MS.gene51394.t1 MTR_2g098880 37.097 124 64 5 35 149 61 179 4.01e-17 81.3
MS.gene51394.t1 MTR_7g061980 35.294 119 71 3 40 155 4 119 4.72e-17 77.4
MS.gene51394.t1 MTR_6g051425 28.205 195 133 2 47 236 18 210 2.83e-16 80.1
MS.gene51394.t1 MTR_4g102720 29.310 174 85 4 41 212 2 139 1.50e-15 75.9
MS.gene51394.t1 MTR_1g013060 31.429 175 110 3 40 206 18 190 2.37e-15 75.5
MS.gene51394.t1 MTR_1g070355 27.957 186 127 3 51 231 79 262 3.36e-15 78.2
MS.gene51394.t1 MTR_7g113010 34.375 128 66 3 46 170 457 569 1.18e-14 76.3
MS.gene51394.t1 MTR_5g076070 26.415 212 138 6 6 205 10 215 1.80e-14 73.9
MS.gene51394.t1 MTR_3g006370 42.202 109 58 3 46 150 50 157 2.15e-14 72.8
MS.gene51394.t1 MTR_7g446520 29.224 219 133 7 3 218 46 245 8.51e-14 72.4
MS.gene51394.t1 MTR_6g022080 26.012 173 118 4 38 205 80 247 1.56e-13 70.5
MS.gene51394.t1 MTR_0137s0040 38.095 105 59 2 25 129 15 113 2.14e-13 66.6
MS.gene51394.t1 MTR_2g014100 28.649 185 112 3 61 225 59 243 2.35e-12 67.0
MS.gene51394.t1 MTR_4g035820 24.519 416 229 13 32 367 61 471 4.03e-12 68.6
MS.gene51394.t1 MTR_3g450850 28.571 182 116 5 42 210 43 223 5.82e-12 67.0
MS.gene51394.t1 MTR_1g008290 37.255 102 62 2 130 230 33 133 1.20e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51394.t1 AT4G38180 34.101 217 131 4 19 224 40 255 5.34e-26 111
MS.gene51394.t1 AT3G59470 42.593 108 60 2 46 152 70 176 5.02e-21 92.4
MS.gene51394.t1 AT3G59470 42.593 108 60 2 46 152 70 176 5.02e-21 92.4
MS.gene51394.t1 AT3G59470 42.593 108 60 2 46 152 82 188 7.26e-21 92.0
MS.gene51394.t1 AT3G07500 42.735 117 60 4 46 156 33 148 9.83e-19 85.1
MS.gene51394.t1 AT5G18960 44.118 102 45 2 46 147 43 132 2.76e-18 88.2
MS.gene51394.t1 AT5G18960 39.604 101 49 2 46 146 211 299 4.39e-15 78.2
MS.gene51394.t1 AT5G18960 44.118 102 45 2 46 147 43 132 2.76e-18 88.2
MS.gene51394.t1 AT5G18960 39.604 101 49 2 46 146 211 299 4.39e-15 78.2
MS.gene51394.t1 AT3G06250 37.302 126 64 3 22 147 7 117 4.69e-17 84.3
MS.gene51394.t1 AT3G06250 27.751 209 109 6 30 237 174 341 2.43e-16 82.0
MS.gene51394.t1 AT3G06250 37.302 126 64 3 22 147 7 117 4.69e-17 84.3
MS.gene51394.t1 AT3G06250 27.751 209 109 6 30 237 174 341 2.43e-16 82.0
MS.gene51394.t1 AT3G06250 37.302 126 64 3 22 147 7 117 4.69e-17 84.3
MS.gene51394.t1 AT3G06250 27.751 209 109 6 30 237 174 341 2.43e-16 82.0
MS.gene51394.t1 AT1G10240 35.971 139 83 2 45 177 47 185 8.27e-17 83.2
MS.gene51394.t1 AT1G10240 35.971 139 83 2 45 177 47 185 8.27e-17 83.2
MS.gene51394.t1 AT4G12850 37.705 122 72 3 46 165 11 130 9.22e-16 74.3
MS.gene51394.t1 AT4G12850 39.604 101 59 2 46 146 11 109 1.81e-14 70.9
MS.gene51394.t1 AT4G12850 39.604 101 59 2 46 146 11 109 2.36e-14 71.6
MS.gene51394.t1 AT5G28530 28.902 173 110 5 46 209 55 223 3.31e-14 75.1
MS.gene51394.t1 AT5G28530 28.902 173 110 5 46 209 55 223 3.44e-14 75.1
MS.gene51394.t1 AT5G28530 28.571 175 112 5 46 211 55 225 3.72e-14 75.1
MS.gene51394.t1 AT5G28530 28.571 175 112 5 46 211 55 225 3.72e-14 75.1
MS.gene51394.t1 AT5G28530 28.571 175 112 5 46 211 55 225 3.83e-14 75.1
MS.gene51394.t1 AT4G15090 29.851 201 129 6 28 223 36 229 5.64e-14 74.7
MS.gene51394.t1 AT4G15090 29.851 201 129 6 28 223 36 229 5.64e-14 74.7
MS.gene51394.t1 AT4G15090 29.851 201 129 6 28 223 36 229 5.64e-14 74.7
MS.gene51394.t1 AT4G15090 29.851 201 129 6 28 223 36 229 5.64e-14 74.7
MS.gene51394.t1 AT4G15090 29.851 201 129 6 28 223 36 229 5.64e-14 74.7
MS.gene51394.t1 AT2G43280 35.036 137 79 6 44 174 20 152 8.25e-12 64.7
MS.gene51394.t1 AT1G52520 38.679 106 56 4 46 148 85 184 1.07e-11 67.4

Find 69 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTTGCATCCATTTATTT+TGG 0.182893 4.1:-52233386 None:intergenic
GGTAATTTATGCTTACTTCT+AGG 0.207796 4.1:-52232598 None:intergenic
CCATTAGAATTCATATTTAC+TGG 0.226524 4.1:-52231786 None:intergenic
TCTTGATATGTATGTGAAAA+TGG 0.253215 4.1:-52234427 None:intergenic
AATTTATATGCTGGCTTTGT+TGG 0.254717 4.1:+52232340 MS.gene51394:CDS
TACATGATGGCTTGTCTTCA+TGG 0.309415 4.1:+52234856 MS.gene51394:CDS
TATTCAATGCAGGCATCTTC+TGG 0.309663 4.1:-52233617 None:intergenic
ACGACCTTCACCAAAATAAA+TGG 0.312043 4.1:+52233376 MS.gene51394:CDS
AATGACATGAATAACGAAAA+TGG 0.314237 4.1:+52234591 MS.gene51394:CDS
GATAAATGACATGCGTTATA+AGG 0.325984 4.1:+52233569 MS.gene51394:CDS
GAAGATGCCTGCATTGAATA+TGG 0.336910 4.1:+52233620 MS.gene51394:CDS
GGAGAACACGGGGAAGAATT+TGG 0.347555 4.1:+52234612 MS.gene51394:CDS
CTTCATTTCCTTCATCCTCT+TGG 0.349382 4.1:-52232779 None:intergenic
TCAAACTCCATGCCAACATA+TGG 0.373528 4.1:-52232289 None:intergenic
GTAATTTATGCTTACTTCTA+GGG 0.397907 4.1:-52232597 None:intergenic
CCAGTAAATATGAATTCTAA+TGG 0.398242 4.1:+52231786 MS.gene51394:CDS
GGGATGCAAGCTCATGATTA+TGG 0.399641 4.1:-52232576 None:intergenic
CGAACTAGAGAAAAGATAAC+TGG 0.408912 4.1:-52233681 None:intergenic
ACATGATGGCTTGTCTTCAT+GGG 0.409074 4.1:+52234857 MS.gene51394:CDS
TCAATTTCAACCAAGTCCTT+TGG 0.409112 4.1:+52234731 MS.gene51394:CDS
AGCAATGTCAATGAACCATT+AGG 0.429016 4.1:+52231747 MS.gene51394:CDS
AGCACATGTTGTCATTAGTC+GGG 0.431183 4.1:+52232513 MS.gene51394:CDS
GGTTTATCGCCACTCCCTAA+TGG 0.444741 4.1:-52231762 None:intergenic
TCTCGTAAGGATATCAGAAC+TGG 0.455278 4.1:+52232484 MS.gene51394:CDS
TCAAGCAGCTGAGGTAGAAA+TGG 0.462861 4.1:+52232645 MS.gene51394:CDS
TAGCACATGTTGTCATTAGT+CGG 0.467396 4.1:+52232512 MS.gene51394:CDS
GCAATGTCAATGAACCATTA+GGG 0.473025 4.1:+52231748 MS.gene51394:CDS
CAATTAAGTGAGAGCTTCAA+TGG 0.473964 4.1:+52233480 MS.gene51394:CDS
CATTCTACCGATGCTTATCA+TGG 0.489055 4.1:+52234636 MS.gene51394:CDS
GTAGAAATGGCAAATCGATC+TGG 0.489212 4.1:+52232658 MS.gene51394:CDS
AAATCTATATGCATGGTCTT+CGG 0.500652 4.1:-52232314 None:intergenic
GCTTAAAATACCAAAGGACT+TGG 0.501002 4.1:-52234741 None:intergenic
GCGTGAAGAACCATATCAAT+TGG 0.502432 4.1:-52234895 None:intergenic
CTCAACAAATGTAGAGCCTC+AGG 0.502446 4.1:+52234692 MS.gene51394:CDS
GTCTTCATGGGGTGGAAACA+AGG 0.516471 4.1:+52234869 MS.gene51394:CDS
TTCTGCTGCTCAAGCAGCTG+AGG 0.524786 4.1:+52232636 MS.gene51394:CDS
AGCTAGTATTAGTACATCAC+AGG 0.524897 4.1:+52234459 MS.gene51394:CDS
CAAACTCCATGCCAACATAT+GGG 0.526793 4.1:-52232288 None:intergenic
TAGGAATTTAGCTTACATGA+TGG 0.535116 4.1:+52234843 MS.gene51394:intron
TTTAACTGCCAAGAGGATGA+AGG 0.543689 4.1:+52232771 MS.gene51394:CDS
CAAGAGGATGAAGGAAATGA+AGG 0.543859 4.1:+52232780 MS.gene51394:CDS
ATGATATCCATATTCAATGC+AGG 0.546116 4.1:-52233627 None:intergenic
ATAAATTACCATCACAAAGG+AGG 0.551834 4.1:+52232611 MS.gene51394:CDS
AGGATGAAGGAAATGAAGGA+AGG 0.556547 4.1:+52232784 MS.gene51394:CDS
GCGTCAACACGTTGGAGGGA+GGG 0.564822 4.1:+52232711 MS.gene51394:CDS
GAAGATATAATCCCATATGT+TGG 0.564964 4.1:+52232277 MS.gene51394:CDS
TAAATTACCATCACAAAGGA+GGG 0.565763 4.1:+52232612 MS.gene51394:CDS
TAATTTATGCTTACTTCTAG+GGG 0.570523 4.1:-52232596 None:intergenic
GTCAATGAACCATTAGGGAG+TGG 0.572938 4.1:+52231753 MS.gene51394:CDS
TTTATGCGTCAACACGTTGG+AGG 0.575636 4.1:+52232706 MS.gene51394:CDS
AATTATAAAATCTATATGCA+TGG 0.584715 4.1:-52232321 None:intergenic
TGCGTCAACACGTTGGAGGG+AGG 0.585310 4.1:+52232710 MS.gene51394:CDS
CTCTTGTCCATGATAAGCAT+CGG 0.585310 4.1:-52234643 None:intergenic
TATAATCCCATATGTTGGCA+TGG 0.590560 4.1:+52232282 MS.gene51394:CDS
TTATGCGTCAACACGTTGGA+GGG 0.593454 4.1:+52232707 MS.gene51394:CDS
CATGATGGCTTGTCTTCATG+GGG 0.595058 4.1:+52234858 MS.gene51394:CDS
AACTCAGCAGATGGTGCCTG+AGG 0.610239 4.1:-52234708 None:intergenic
GCAGAAACCCTCCTTTGTGA+TGG 0.614369 4.1:-52232619 None:intergenic
TTAGAATTCATATTTACTGG+AGG 0.621664 4.1:-52231783 None:intergenic
AATAACGAAAATGGAGAACA+CGG 0.627085 4.1:+52234600 MS.gene51394:CDS
GTCATTAGTCGGGAATCAAG+TGG 0.642505 4.1:+52232523 MS.gene51394:CDS
AGCATAAATTACCATCACAA+AGG 0.644723 4.1:+52232608 MS.gene51394:CDS
GTTGGAGGGAGGGAGAATGT+TGG 0.645198 4.1:+52232721 MS.gene51394:CDS
GATGGCTTGTCTTCATGGGG+TGG 0.647236 4.1:+52234861 MS.gene51394:CDS
GCAATCATTTAACTGCCAAG+AGG 0.652505 4.1:+52232764 MS.gene51394:CDS
ATAACGAAAATGGAGAACAC+GGG 0.654047 4.1:+52234601 MS.gene51394:CDS
TGAAATTGAAACTCAGCAGA+TGG 0.678366 4.1:-52234717 None:intergenic
GAGTTTATGCGTCAACACGT+TGG 0.685392 4.1:+52232703 MS.gene51394:CDS
TAACGAAAATGGAGAACACG+GGG 0.724055 4.1:+52234602 MS.gene51394:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATAGTGTTATTATTAA+AGG - chr4.1:52232027-52232046 None:intergenic 10.0%
!!! CAATGTTTTTAAATATTTTT+AGG + chr4.1:52231836-52231855 MS.gene51394:intron 10.0%
!! AATTATAAAATCTATATGCA+TGG - chr4.1:52232324-52232343 None:intergenic 15.0%
!! CTATAAGAAAAAAGAAAAAT+GGG + chr4.1:52233410-52233429 MS.gene51394:CDS 15.0%
!! TCTATAAGAAAAAAGAAAAA+TGG + chr4.1:52233409-52233428 MS.gene51394:CDS 15.0%
!!! AGATTTTATAATTTATATGC+TGG + chr4.1:52232331-52232350 MS.gene51394:CDS 15.0%
!!! TTTTGGATTATGTTTATTTT+GGG + chr4.1:52232932-52232951 MS.gene51394:intron 15.0%
!! AAAAAATACAAAAGAAGCAA+AGG + chr4.1:52234237-52234256 MS.gene51394:intron 20.0%
!! AAATTCTAACACATTATAAG+TGG - chr4.1:52233529-52233548 None:intergenic 20.0%
!! ATCTATTATAAACAAGCAAT+CGG - chr4.1:52232172-52232191 None:intergenic 20.0%
!! TAAACATTTAACATGAACAT+AGG - chr4.1:52232121-52232140 None:intergenic 20.0%
!!! AAGCAAATTTTTTTAATCAG+TGG + chr4.1:52234803-52234822 MS.gene51394:intron 20.0%
!!! AAGTTTGTTTGTTTTAAAGA+AGG + chr4.1:52232427-52232446 MS.gene51394:CDS 20.0%
!!! AATATGTAGTAAGTTTTTTC+AGG + chr4.1:52233769-52233788 MS.gene51394:intron 20.0%
!!! ATATGTAGTAAGTTTTTTCA+GGG + chr4.1:52233770-52233789 MS.gene51394:intron 20.0%
!!! ATTTTCTTATTCACAGTTAA+AGG + chr4.1:52232223-52232242 MS.gene51394:intron 20.0%
!!! CATTTATGATTTTGAATATG+AGG + chr4.1:52233314-52233333 MS.gene51394:intron 20.0%
!!! GTTGTTTAAAACTTTTCTTA+AGG + chr4.1:52233098-52233117 MS.gene51394:intron 20.0%
!!! GTTTTGGATTATGTTTATTT+TGG + chr4.1:52232931-52232950 MS.gene51394:intron 20.0%
!!! TTTGGATTATGTTTATTTTG+GGG + chr4.1:52232933-52232952 MS.gene51394:intron 20.0%
!!! TTTTCTTATTCACAGTTAAA+GGG + chr4.1:52232224-52232243 MS.gene51394:intron 20.0%
! AATGACATGAATAACGAAAA+TGG + chr4.1:52234591-52234610 MS.gene51394:CDS 25.0%
! CCAGTAAATATGAATTCTAA+TGG + chr4.1:52231786-52231805 MS.gene51394:CDS 25.0%
! CCATTAGAATTCATATTTAC+TGG - chr4.1:52231789-52231808 None:intergenic 25.0%
! CTAGTTATAGTTGTTTATGT+TGG + chr4.1:52231941-52231960 MS.gene51394:intron 25.0%
! GTAATTTATGCTTACTTCTA+GGG - chr4.1:52232600-52232619 None:intergenic 25.0%
! TAATTTATGCTTACTTCTAG+GGG - chr4.1:52232599-52232618 None:intergenic 25.0%
! TCTTGATATGTATGTGAAAA+TGG - chr4.1:52234430-52234449 None:intergenic 25.0%
! TTAGAATTCATATTTACTGG+AGG - chr4.1:52231786-52231805 None:intergenic 25.0%
!! AAAAATGGGCTTTAGTATAT+GGG + chr4.1:52233424-52233443 MS.gene51394:CDS 25.0%
!! ATCAAATCACAAACATTTTC+TGG + chr4.1:52232893-52232912 MS.gene51394:intron 25.0%
!! TCAAATCACAAACATTTTCT+GGG + chr4.1:52232894-52232913 MS.gene51394:intron 25.0%
!!! ACATAGGTTTTTTTCTAGTT+TGG - chr4.1:52232105-52232124 None:intergenic 25.0%
!!! ATCAGTGGTATATTTTTTGT+TGG + chr4.1:52234818-52234837 MS.gene51394:intron 25.0%
!!! GTTTTAAAGAAGGTTTTAAG+AGG + chr4.1:52232437-52232456 MS.gene51394:CDS 25.0%
!!! GTTTTAGCATTAGAAAAGAT+TGG + chr4.1:52232362-52232381 MS.gene51394:CDS 25.0%
AAAAGAATCTGATCTCTGAA+AGG - chr4.1:52233274-52233293 None:intergenic 30.0%
AAATCTATATGCATGGTCTT+CGG - chr4.1:52232317-52232336 None:intergenic 30.0%
AATAACGAAAATGGAGAACA+CGG + chr4.1:52234600-52234619 MS.gene51394:CDS 30.0%
AATCCAATAAAAGGAGCAAA+TGG - chr4.1:52233006-52233025 None:intergenic 30.0%
ACACACTACAAGAAAACAAA+GGG + chr4.1:52231981-52232000 MS.gene51394:intron 30.0%
ACTTTGATCTTCAGAGTTAA+AGG - chr4.1:52233817-52233836 None:intergenic 30.0%
AGCATAAATTACCATCACAA+AGG + chr4.1:52232608-52232627 MS.gene51394:CDS 30.0%
ATAAATTACCATCACAAAGG+AGG + chr4.1:52232611-52232630 MS.gene51394:CDS 30.0%
ATACTCAGAAATTGATCGAT+AGG + chr4.1:52231864-52231883 MS.gene51394:intron 30.0%
ATATCACACTTTCTCTATGA+TGG - chr4.1:52233032-52233051 None:intergenic 30.0%
ATCATCTGTTACTATCTCAT+TGG - chr4.1:52234133-52234152 None:intergenic 30.0%
ATGATATCCATATTCAATGC+AGG - chr4.1:52233630-52233649 None:intergenic 30.0%
ATGGGTATAAAAAAGAAGGA+TGG + chr4.1:52234174-52234193 MS.gene51394:intron 30.0%
GAAGATATAATCCCATATGT+TGG + chr4.1:52232277-52232296 MS.gene51394:CDS 30.0%
GATAAATGACATGCGTTATA+AGG + chr4.1:52233569-52233588 MS.gene51394:CDS 30.0%
GGTAATTTATGCTTACTTCT+AGG - chr4.1:52232601-52232620 None:intergenic 30.0%
TAAATTACCATCACAAAGGA+GGG + chr4.1:52232612-52232631 MS.gene51394:CDS 30.0%
TGATCTGTGAAAATTGTCTT+CGG - chr4.1:52233141-52233160 None:intergenic 30.0%
TGATGGTTGAATCCAATAAA+AGG - chr4.1:52233015-52233034 None:intergenic 30.0%
TTATGTAGTACCTGCATAAT+TGG - chr4.1:52234309-52234328 None:intergenic 30.0%
TTGAGTGCTTAAAATACCAA+AGG - chr4.1:52234750-52234769 None:intergenic 30.0%
! AAAAGTACATATTGAAGAGG+TGG + chr4.1:52233931-52233950 MS.gene51394:intron 30.0%
! ACCTCTTCAATATGTACTTT+TGG - chr4.1:52233932-52233951 None:intergenic 30.0%
! CATAGAGAAAGTGTGATATT+TGG + chr4.1:52233033-52233052 MS.gene51394:intron 30.0%
! CCTCTTCAATATGTACTTTT+GGG - chr4.1:52233931-52233950 None:intergenic 30.0%
! CTCTTCAATATGTACTTTTG+GGG - chr4.1:52233930-52233949 None:intergenic 30.0%
! GAAAAATGGGCTTTAGTATA+TGG + chr4.1:52233423-52233442 MS.gene51394:CDS 30.0%
! GATCTTGCATCCATTTATTT+TGG - chr4.1:52233389-52233408 None:intergenic 30.0%
! TAGGAATTTAGCTTACATGA+TGG + chr4.1:52234843-52234862 MS.gene51394:intron 30.0%
!! AATTTATATGCTGGCTTTGT+TGG + chr4.1:52232340-52232359 MS.gene51394:CDS 30.0%
!!! ATCCTTCTTTTTTATACCCA+TGG - chr4.1:52234175-52234194 None:intergenic 30.0%
!!! GGTATATTTTTTGTTGGTGT+AGG + chr4.1:52234824-52234843 MS.gene51394:intron 30.0%
!!! TGTCGACTTTTTCAATTCAT+TGG + chr4.1:52233849-52233868 MS.gene51394:intron 30.0%
ACGACCTTCACCAAAATAAA+TGG + chr4.1:52233376-52233395 MS.gene51394:CDS 35.0%
AGAAATTGATCGATAGGAGT+TGG + chr4.1:52231870-52231889 MS.gene51394:intron 35.0%
AGAAGATCCAAAGATAGTTG+TGG + chr4.1:52234007-52234026 MS.gene51394:intron 35.0%
AGCAATGTCAATGAACCATT+AGG + chr4.1:52231747-52231766 MS.gene51394:CDS 35.0%
AGCCATGGGTATAAAAAAGA+AGG + chr4.1:52234170-52234189 MS.gene51394:intron 35.0%
AGCTAGTATTAGTACATCAC+AGG + chr4.1:52234459-52234478 MS.gene51394:CDS 35.0%
AGTATTAGTACATCACAGGT+TGG + chr4.1:52234463-52234482 MS.gene51394:intron 35.0%
ATAACGAAAATGGAGAACAC+GGG + chr4.1:52234601-52234620 MS.gene51394:CDS 35.0%
ATCAACAACAAATCTTGGTG+AGG + chr4.1:52234095-52234114 MS.gene51394:intron 35.0%
ATCATATCCCCTTCAAATGT+AGG + chr4.1:52234566-52234585 MS.gene51394:intron 35.0%
CAATTAAGTGAGAGCTTCAA+TGG + chr4.1:52233480-52233499 MS.gene51394:CDS 35.0%
CACACACTACAAGAAAACAA+AGG + chr4.1:52231980-52231999 MS.gene51394:intron 35.0%
CATATCATCGTTTATGCTTG+TGG + chr4.1:52233211-52233230 MS.gene51394:intron 35.0%
CGAACTAGAGAAAAGATAAC+TGG - chr4.1:52233684-52233703 None:intergenic 35.0%
CGATGATATGTTTCACTTTC+AGG - chr4.1:52233201-52233220 None:intergenic 35.0%
GATGGTAAATCACGCATAAA+AGG + chr4.1:52234192-52234211 MS.gene51394:intron 35.0%
GCAATGTCAATGAACCATTA+GGG + chr4.1:52231748-52231767 MS.gene51394:CDS 35.0%
GCTTAAAATACCAAAGGACT+TGG - chr4.1:52234744-52234763 None:intergenic 35.0%
GTCGTAGATTCTTATTAGTG+CGG - chr4.1:52232984-52233003 None:intergenic 35.0%
TAGCACATGTTGTCATTAGT+CGG + chr4.1:52232512-52232531 MS.gene51394:CDS 35.0%
TATAATCCCATATGTTGGCA+TGG + chr4.1:52232282-52232301 MS.gene51394:CDS 35.0%
TCAATTTCAACCAAGTCCTT+TGG + chr4.1:52234731-52234750 MS.gene51394:CDS 35.0%
TCCACATCAACAACAAATCT+TGG + chr4.1:52234090-52234109 MS.gene51394:intron 35.0%
TGAAATTGAAACTCAGCAGA+TGG - chr4.1:52234720-52234739 None:intergenic 35.0%
! ACTTTTCTTAAGGCAATGTG+TGG + chr4.1:52233108-52233127 MS.gene51394:intron 35.0%
! CCCAAAAGTACATATTGAAG+AGG + chr4.1:52233928-52233947 MS.gene51394:intron 35.0%
! GAGATCAGATTCTTTTGCTA+TGG + chr4.1:52233278-52233297 MS.gene51394:intron 35.0%
! GTCATTAGCCTACATTTGAA+GGG - chr4.1:52234577-52234596 None:intergenic 35.0%
! TCATTAGCCTACATTTGAAG+GGG - chr4.1:52234576-52234595 None:intergenic 35.0%
! TGTCATTAGCCTACATTTGA+AGG - chr4.1:52234578-52234597 None:intergenic 35.0%
! TTTTTCTCCACACAACTCTT+AGG - chr4.1:52234222-52234241 None:intergenic 35.0%
!! ACCAAGATTTGTTGTTGATG+TGG - chr4.1:52234094-52234113 None:intergenic 35.0%
!! GTTATCTTTTCTCTAGTTCG+TGG + chr4.1:52233684-52233703 MS.gene51394:CDS 35.0%
!! TGATGAAAAAGTGTCTCGTA+AGG + chr4.1:52232471-52232490 MS.gene51394:CDS 35.0%
!!! GCATCCATTTATTTTGGTGA+AGG - chr4.1:52233383-52233402 None:intergenic 35.0%
!!! TAAGTTTTTTCAGGGACATG+AGG + chr4.1:52233778-52233797 MS.gene51394:intron 35.0%
AAACTGCAGCATCATTTGAG+TGG + chr4.1:52233076-52233095 MS.gene51394:intron 40.0%
AACCATCATGCCAATTATGC+AGG + chr4.1:52234296-52234315 MS.gene51394:intron 40.0%
ACCACTGTATCAAAAGCCAT+GGG + chr4.1:52234156-52234175 MS.gene51394:intron 40.0%
AGCACATGTTGTCATTAGTC+GGG + chr4.1:52232513-52232532 MS.gene51394:CDS 40.0%
AGGATGAAGGAAATGAAGGA+AGG + chr4.1:52232784-52232803 MS.gene51394:CDS 40.0%
CAAACTCCATGCCAACATAT+GGG - chr4.1:52232291-52232310 None:intergenic 40.0%
CAAGAGGATGAAGGAAATGA+AGG + chr4.1:52232780-52232799 MS.gene51394:CDS 40.0%
CATGGCAAAAGCAATTTCAG+AGG + chr4.1:52233170-52233189 MS.gene51394:intron 40.0%
CATTCTACCGATGCTTATCA+TGG + chr4.1:52234636-52234655 MS.gene51394:CDS 40.0%
CGATTTGCCACAACTATCTT+TGG - chr4.1:52234017-52234036 None:intergenic 40.0%
CTCTTGTCCATGATAAGCAT+CGG - chr4.1:52234646-52234665 None:intergenic 40.0%
CTTCATTTCCTTCATCCTCT+TGG - chr4.1:52232782-52232801 None:intergenic 40.0%
GAAGATGCCTGCATTGAATA+TGG + chr4.1:52233620-52233639 MS.gene51394:CDS 40.0%
GCAATCATTTAACTGCCAAG+AGG + chr4.1:52232764-52232783 MS.gene51394:CDS 40.0%
GTAAATCACGCATAAAAGGC+AGG + chr4.1:52234196-52234215 MS.gene51394:intron 40.0%
GTAGAAATGGCAAATCGATC+TGG + chr4.1:52232658-52232677 MS.gene51394:CDS 40.0%
TAACGAAAATGGAGAACACG+GGG + chr4.1:52234602-52234621 MS.gene51394:CDS 40.0%
TACCACTGTATCAAAAGCCA+TGG + chr4.1:52234155-52234174 MS.gene51394:intron 40.0%
TACCTGCATAATTGGCATGA+TGG - chr4.1:52234301-52234320 None:intergenic 40.0%
TATTCAATGCAGGCATCTTC+TGG - chr4.1:52233620-52233639 None:intergenic 40.0%
TCAAACTCCATGCCAACATA+TGG - chr4.1:52232292-52232311 None:intergenic 40.0%
TCTCGTAAGGATATCAGAAC+TGG + chr4.1:52232484-52232503 MS.gene51394:CDS 40.0%
TTTAACTGCCAAGAGGATGA+AGG + chr4.1:52232771-52232790 MS.gene51394:CDS 40.0%
TTTCTGGGCAGATGCTAAAA+TGG + chr4.1:52232909-52232928 MS.gene51394:intron 40.0%
! ACATGATGGCTTGTCTTCAT+GGG + chr4.1:52234857-52234876 MS.gene51394:CDS 40.0%
! CGACCATTTGCTCCTTTTAT+TGG + chr4.1:52233000-52233019 MS.gene51394:intron 40.0%
! GGCAGATGCTAAAATGGTTT+TGG + chr4.1:52232915-52232934 MS.gene51394:intron 40.0%
! TACATGATGGCTTGTCTTCA+TGG + chr4.1:52234856-52234875 MS.gene51394:CDS 40.0%
!!! CTCTGAAATTGCTTTTGCCA+TGG - chr4.1:52233172-52233191 None:intergenic 40.0%
AGGATGACTTTCTTGCAGCT+TGG + chr4.1:52233334-52233353 MS.gene51394:intron 45.0%
CTCAACAAATGTAGAGCCTC+AGG + chr4.1:52234692-52234711 MS.gene51394:CDS 45.0%
GAGTTTATGCGTCAACACGT+TGG + chr4.1:52232703-52232722 MS.gene51394:CDS 45.0%
GATCTATGTCGCACTACAGT+AGG + chr4.1:52234044-52234063 MS.gene51394:intron 45.0%
GGATGTGTGCTAGACAGTAA+GGG + chr4.1:52233972-52233991 MS.gene51394:intron 45.0%
GGGATGCAAGCTCATGATTA+TGG - chr4.1:52232579-52232598 None:intergenic 45.0%
GTCAATGAACCATTAGGGAG+TGG + chr4.1:52231753-52231772 MS.gene51394:CDS 45.0%
GTCATTAGTCGGGAATCAAG+TGG + chr4.1:52232523-52232542 MS.gene51394:CDS 45.0%
GTTGGTCAGTAGGTTAGAGA+TGG + chr4.1:52231888-52231907 MS.gene51394:intron 45.0%
TCAAGCAGCTGAGGTAGAAA+TGG + chr4.1:52232645-52232664 MS.gene51394:CDS 45.0%
TGGATGTGTGCTAGACAGTA+AGG + chr4.1:52233971-52233990 MS.gene51394:intron 45.0%
TTATGCGTCAACACGTTGGA+GGG + chr4.1:52232707-52232726 MS.gene51394:CDS 45.0%
TTTATGCGTCAACACGTTGG+AGG + chr4.1:52232706-52232725 MS.gene51394:CDS 45.0%
! ACCCATGGCTTTTGATACAG+TGG - chr4.1:52234160-52234179 None:intergenic 45.0%
! ATCGATAGGAGTTGGTCAGT+AGG + chr4.1:52231878-52231897 MS.gene51394:intron 45.0%
! CATGATGGCTTGTCTTCATG+GGG + chr4.1:52234858-52234877 MS.gene51394:CDS 45.0%
! CTCGAAGAGCATGACAACAA+AGG - chr4.1:52233878-52233897 None:intergenic 45.0%
!! AGAGGTGGACTAAACAAGCT+CGG + chr4.1:52233946-52233965 MS.gene51394:intron 45.0%
!! AAGAAAATATATAAATTTAA+TGG - chr4.1:52232211-52232230 None:intergenic 5.0%
CACAGATCAAGATGCTGCCA+TGG + chr4.1:52233152-52233171 MS.gene51394:intron 50.0%
GCAGAAACCCTCCTTTGTGA+TGG - chr4.1:52232622-52232641 None:intergenic 50.0%
GGAGAACACGGGGAAGAATT+TGG + chr4.1:52234612-52234631 MS.gene51394:CDS 50.0%
GGTTTATCGCCACTCCCTAA+TGG - chr4.1:52231765-52231784 None:intergenic 50.0%
GTCTTCATGGGGTGGAAACA+AGG + chr4.1:52234869-52234888 MS.gene51394:CDS 50.0%
TGGACTAAACAAGCTCGGAG+TGG + chr4.1:52233951-52233970 MS.gene51394:intron 50.0%
! GGGCGAAACACATTTTCAGC+TGG + chr4.1:52233444-52233463 MS.gene51394:CDS 50.0%
AACTCAGCAGATGGTGCCTG+AGG - chr4.1:52234711-52234730 None:intergenic 55.0%
AGGCAGGCCTAAGAGTTGTG+TGG + chr4.1:52234212-52234231 MS.gene51394:intron 55.0%
GTTGGAGGGAGGGAGAATGT+TGG + chr4.1:52232721-52232740 MS.gene51394:CDS 55.0%
TTCTGCTGCTCAAGCAGCTG+AGG + chr4.1:52232636-52232655 MS.gene51394:CDS 55.0%
! GATGGCTTGTCTTCATGGGG+TGG + chr4.1:52234861-52234880 MS.gene51394:CDS 55.0%
GCGTCAACACGTTGGAGGGA+GGG + chr4.1:52232711-52232730 MS.gene51394:CDS 60.0%
TGCGTCAACACGTTGGAGGG+AGG + chr4.1:52232710-52232729 MS.gene51394:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 52231723 52234902 52231723 ID=MS.gene51394
chr4.1 mRNA 52231723 52234902 52231723 ID=MS.gene51394.t1;Parent=MS.gene51394
chr4.1 exon 52231723 52231807 52231723 ID=MS.gene51394.t1.exon1;Parent=MS.gene51394.t1
chr4.1 CDS 52231723 52231807 52231723 ID=cds.MS.gene51394.t1;Parent=MS.gene51394.t1
chr4.1 exon 52232239 52232805 52232239 ID=MS.gene51394.t1.exon2;Parent=MS.gene51394.t1
chr4.1 CDS 52232239 52232805 52232239 ID=cds.MS.gene51394.t1;Parent=MS.gene51394.t1
chr4.1 exon 52233352 52233504 52233352 ID=MS.gene51394.t1.exon3;Parent=MS.gene51394.t1
chr4.1 CDS 52233352 52233504 52233352 ID=cds.MS.gene51394.t1;Parent=MS.gene51394.t1
chr4.1 exon 52233562 52233776 52233562 ID=MS.gene51394.t1.exon4;Parent=MS.gene51394.t1
chr4.1 CDS 52233562 52233776 52233562 ID=cds.MS.gene51394.t1;Parent=MS.gene51394.t1
chr4.1 exon 52234421 52234480 52234421 ID=MS.gene51394.t1.exon5;Parent=MS.gene51394.t1
chr4.1 CDS 52234421 52234480 52234421 ID=cds.MS.gene51394.t1;Parent=MS.gene51394.t1
chr4.1 exon 52234588 52234752 52234588 ID=MS.gene51394.t1.exon6;Parent=MS.gene51394.t1
chr4.1 CDS 52234588 52234752 52234588 ID=cds.MS.gene51394.t1;Parent=MS.gene51394.t1
chr4.1 exon 52234846 52234902 52234846 ID=MS.gene51394.t1.exon7;Parent=MS.gene51394.t1
chr4.1 CDS 52234846 52234902 52234846 ID=cds.MS.gene51394.t1;Parent=MS.gene51394.t1
Gene Sequence

>MS.gene51394

ATGGAAAATTCAAATTTCACTGCAAGCAATGTCAATGAACCATTAGGGAGTGGCGATAAACCTCCAGTAAATATGAATTCTAATGGTATGTATTTTTCTATTTAAATTATATTCAATGTTTTTAAATATTTTTAGGAGAAGATACTCAGAAATTGATCGATAGGAGTTGGTCAGTAGGTTAGAGATGGTACAAATTGATAACATAATAAAGCATTGTTCTAGTTATAGTTGTTTATGTTGGTACTGTCATAGAATAACACACACTACAAGAAAACAAAGGGTTCATAGAGACATAACTACACCTTTAATAATAACACTATAATTGTAATTTATAACAACGTTTAAATTCATAATAATAGTAACATTGAAGCTTTATCTACCAAACTAGAAAAAAACCTATGTTCATGTTAAATGTTTATGTATTGCTTTTTCTTATAATATGTTTACCGATTGCTTGTTTATAATAGATTGAGCTGATGATTTAACCATTAAATTTATATATTTTCTTATTCACAGTTAAAGGGAATGATAATCTTGAGCATGAACTAACTGAAGAAGATATAATCCCATATGTTGGCATGGAGTTTGATACCGAAGACCATGCATATAGATTTTATAATTTATATGCTGGCTTTGTTGGTTTTAGCATTAGAAAAGATTGGAGAAACAAAAGCAAATTAGATAATGAAACTGTGATGTCTCGAAAGTTTGTTTGTTTTAAAGAAGGTTTTAAGAGGATCAAAGAATATGATGAAAAAGTGTCTCGTAAGGATATCAGAACTGGATGTTTAGCACATGTTGTCATTAGTCGGGAATCAAGTGGAAAATATGTTGTTACATCTTTCAGAAAAGACCATAATCATGAGCTTGCATCCCCTAGAAGTAAGCATAAATTACCATCACAAAGGAGGGTTTCTGCTGCTCAAGCAGCTGAGGTAGAAATGGCAAATCGATCTGGTATACGTCAAAAATTGATTTTTGAGTTTATGCGTCAACACGTTGGAGGGAGGGAGAATGTTGGTTGCACTTCTAAAGATATTAGCAATCATTTAACTGCCAAGAGGATGAAGGAAATGAAGGAAGGTGAAGCATATACTTTGCTTCATTATTTTAAAGCAAAGCAAACAGAGAATCAATCATTCTTTTATGATATTCAACTTGATGTTGATAATCAAATCACAAACATTTTCTGGGCAGATGCTAAAATGGTTTTGGATTATGTTTATTTTGGGGATGTAGTATGTTTTGATACAACATACCGCACTAATAAGAATCTACGACCATTTGCTCCTTTTATTGGATTCAACCATCATAGAGAAAGTGTGATATTTGGTGCAGCATTGTTATACGATGAAACTGCAGCATCATTTGAGTGGTTGTTTAAAACTTTTCTTAAGGCAATGTGTGGAAAAAAGCCGAAGACAATTTTCACAGATCAAGATGCTGCCATGGCAAAAGCAATTTCAGAGGTTTTTCCTGAAAGTGAAACATATCATCGTTTATGCTTGTGGCATTTATTTCAGAATGCACTTAAGAATCTTAATCATGCCTTTCAGAGATCAGATTCTTTTGCTATGGATTTGAGAAGTTGCATTTATGATTTTGAATATGAGGATGACTTTCTTGCAGCTTGGAATTCGTTACTTGAAAAGCACGACCTTCACCAAAATAAATGGATGCAAGATCTCTATAAGAAAAAAGAAAAATGGGCTTTAGTATATGGGCGAAACACATTTTCAGCTGGTGCTACAACAACACAATTAAGTGAGAGCTTCAATGGTCGTTTACGACTTTACATGAAGTCCACTTATAATGTGTTAGAATTTTTCACACACTTAGAAAGATTGATAAATGACATGCGTTATAAGGAGATAGAGTCTAAATATGAAATGAGCCAGAAGATGCCTGCATTGAATATGGATATCATGTTACTCAAAACTGCTAGAGATATCTACACACCAGTTATCTTTTCTCTAGTTCGTGGAGAATATGAAAAGTCTTGTAATCTTCTTTTGAACAACTGCTCTAAAAACTTGCAATTATATGAATATGTAGTAAGTTTTTTCAGGGACATGAGGCAGCATAAAGTTACCTTTAACTCTGAAGATCAAAGTGTTGAGTGCAGTTGTCGACTTTTTCAATTCATTGGAATCCTTTGTTGTCATGCTCTTCGAGTACTTAATCATCAAAATATAATAGTTTTTCCCCAAAAGTACATATTGAAGAGGTGGACTAAACAAGCTCGGAGTGGATGTGTGCTAGACAGTAAGGGACAAATTATCAAAGAAGATCCAAAGATAGTTGTGGCAAATCGCTTCAAAGATCTATGTCGCACTACAGTAGGAATAAATCTTAAGCAAAAGATCTTCCACATCAACAACAAATCTTGGTGAGGTAAATAGTCGAACCAATGAGATAGTAACAGATGATATTACCACTGTATCAAAAGCCATGGGTATAAAAAAGAAGGATGGTAAATCACGCATAAAAGGCAGGCCTAAGAGTTGTGTGGAGAAAAAATACAAAAGAAGCAAAGGCACTCACAAGCGTAGTCATGTGACACAAAATTATTCAACCATCATGCCAATTATGCAGGTACTACATAATAGTAACTCATATTTCTTTGTTTGAGTATAAATTATTTTGTTCAATACTTTATCTATTAAATTAAATATGTTTTTGATTGTTGTATGTGCAGAATTTTCCATTTTCACATACATATCAAGATCTAGAAGCAGCTAGTATTAGTACATCACAGGTTGGATTATTCATCACACAATTTATTTTTCTTTTTTGTACTTGCAGATTTTTTTTTTGTTCAGCTTTATATGTGTGTCATACTCATCATATCCCCTTCAAATGTAGGCTAATGACATGAATAACGAAAATGGAGAACACGGGGAAGAATTTGGTCATTCTACCGATGCTTATCATGGACAAGAGCATCTGAGTCAAAGTCGCATGCTTAACTCAACAAATGTAGAGCCTCAGGCACCATCTGCTGAGTTTCAATTTCAACCAAGTCCTTTGGTATTTTAAGCACTCAATGCTTTATCACTAATGTTGTCAACACTCATTACAAGCAAATTTTTTTAATCAGTGGTATATTTTTTGTTGGTGTAGGAATTTAGCTTACATGATGGCTTGTCTTCATGGGGTGGAAACAAGGAGACCAATTGA
Protein sequence

>MS.gene51394.t1

MENSNFTASNVNEPLGSGDKPPVNMNSNVKGNDNLEHELTEEDIIPYVGMEFDTEDHAYRFYNLYAGFVGFSIRKDWRNKSKLDNETVMSRKFVCFKEGFKRIKEYDEKVSRKDIRTGCLAHVVISRESSGKYVVTSFRKDHNHELASPRSKHKLPSQRRVSAAQAAEVEMANRSGIRQKLIFEFMRQHVGGRENVGCTSKDISNHLTAKRMKEMKEAWNSLLEKHDLHQNKWMQDLYKKKEKWALVYGRNTFSAGATTTQLSESFNGQRLINDMRYKEIESKYEMSQKMPALNMDIMLLKTARDIYTPVIFSLVRGEYEKSCNLLLNNCSKNLQLYEYVNFPFSHTYQDLEAASISTSQANDMNNENGEHGEEFGHSTDAYHGQEHLSQSRMLNSTNVEPQAPSAEFQFQPSPLEFSLHDGLSSWGGNKETN