AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51703

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51703.t1 MTR_3g010660 97.205 322 9 0 1 322 1 322 0.0 647
MS.gene51703.t1 MTR_8g043960 65.260 308 92 4 16 322 20 313 4.90e-137 391
MS.gene51703.t1 MTR_7g088090 49.216 319 128 9 20 320 16 318 2.57e-83 254
MS.gene51703.t1 MTR_6g026800 50.467 321 128 9 19 322 15 321 4.32e-80 246
MS.gene51703.t1 MTR_5g088230 44.886 176 81 5 1 170 1 166 4.52e-34 126
MS.gene51703.t1 MTR_5g088230 57.955 88 37 0 235 322 182 269 6.22e-23 96.3
MS.gene51703.t1 MTR_2g086390 33.968 315 139 10 23 320 11 273 1.05e-33 125
MS.gene51703.t1 MTR_2g086390 34.256 289 127 9 23 294 11 253 1.54e-33 125
MS.gene51703.t1 MTR_5g088230 45.455 176 75 6 1 170 1 161 7.10e-33 122
MS.gene51703.t1 MTR_5g088230 57.955 88 37 0 235 322 177 264 6.74e-23 96.3
MS.gene51703.t1 MTR_2g086340 36.735 294 152 10 12 294 14 284 1.50e-28 112
MS.gene51703.t1 MTR_3g101780 48.361 122 56 2 19 138 26 142 1.45e-26 108
MS.gene51703.t1 MTR_3g101780 63.333 60 22 0 238 297 339 398 7.11e-17 81.3
MS.gene51703.t1 MTR_4g085910 43.182 132 63 3 20 144 18 144 1.99e-24 102
MS.gene51703.t1 MTR_4g085910 68.421 57 17 1 246 301 296 352 2.40e-15 76.3
MS.gene51703.t1 MTR_5g060990 29.866 298 162 9 16 273 8 298 6.16e-21 91.7
MS.gene51703.t1 MTR_7g104480 43.182 132 56 3 26 138 45 176 3.31e-20 90.9
MS.gene51703.t1 MTR_4g094390 41.748 103 54 2 196 296 77 175 5.87e-14 69.7
MS.gene51703.t1 MTR_8g075130 36.957 138 68 4 194 322 51 178 4.32e-13 67.0
MS.gene51703.t1 MTR_1g098590 62.745 51 19 0 246 296 157 207 2.81e-12 65.9
MS.gene51703.t1 MTR_7g015990 68.000 50 13 1 251 297 120 169 2.92e-12 65.1
MS.gene51703.t1 MTR_1g098590 62.745 51 19 0 246 296 157 207 3.55e-12 65.5
MS.gene51703.t1 MTR_1g098590 62.745 51 19 0 246 296 157 207 4.37e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51703.t1 AT3G56850 52.716 313 119 8 14 322 10 297 7.30e-90 271
MS.gene51703.t1 AT2G41070 48.418 316 101 10 10 322 15 271 1.82e-74 230
MS.gene51703.t1 AT2G41070 48.418 316 101 10 10 322 6 262 2.39e-74 230
MS.gene51703.t1 AT2G41070 48.418 316 101 10 10 322 6 262 2.39e-74 230
MS.gene51703.t1 AT2G41070 48.418 316 101 10 10 322 6 262 2.39e-74 230
MS.gene51703.t1 AT2G41070 47.931 290 89 10 10 296 6 236 1.73e-63 201
MS.gene51703.t1 AT2G36270 31.707 410 165 15 12 315 30 430 6.50e-34 129
MS.gene51703.t1 AT2G36270 31.707 410 165 15 12 315 30 430 6.50e-34 129
MS.gene51703.t1 AT2G36270 32.540 378 150 14 12 293 30 398 1.57e-33 128
MS.gene51703.t1 AT1G49720 48.855 131 57 2 18 138 21 151 1.32e-30 119
MS.gene51703.t1 AT1G49720 58.333 60 25 0 237 296 298 357 2.56e-13 70.5
MS.gene51703.t1 AT1G49720 48.855 131 57 2 18 138 21 151 1.66e-30 119
MS.gene51703.t1 AT1G49720 58.333 60 25 0 237 296 298 357 3.19e-13 70.1
MS.gene51703.t1 AT1G49720 48.855 131 57 2 18 138 21 151 1.66e-30 119
MS.gene51703.t1 AT1G49720 58.333 60 25 0 237 296 298 357 3.19e-13 70.1
MS.gene51703.t1 AT3G44460 37.582 306 130 13 14 294 22 291 7.74e-30 116
MS.gene51703.t1 AT3G19290 43.046 151 65 3 9 138 21 171 3.16e-25 105
MS.gene51703.t1 AT3G19290 43.046 151 65 3 9 138 21 171 3.56e-25 105
MS.gene51703.t1 AT3G19290 37.500 136 63 4 183 296 262 397 7.46e-14 72.0
MS.gene51703.t1 AT3G19290 43.046 151 65 3 9 138 21 171 4.02e-25 105
MS.gene51703.t1 AT3G19290 37.500 136 63 4 183 296 262 397 7.96e-14 72.0
MS.gene51703.t1 AT3G19290 43.046 151 65 3 9 138 21 171 4.49e-25 104
MS.gene51703.t1 AT3G19290 37.500 136 63 4 183 296 262 397 8.95e-14 72.0
MS.gene51703.t1 AT3G19290 43.046 151 65 3 9 138 21 171 4.49e-25 104
MS.gene51703.t1 AT3G19290 37.500 136 63 4 183 296 262 397 8.95e-14 72.0
MS.gene51703.t1 AT5G42910 28.455 369 174 12 20 319 20 367 8.82e-23 97.8
MS.gene51703.t1 AT4G34000 34.715 193 77 6 19 171 26 209 2.17e-21 94.7
MS.gene51703.t1 AT4G34000 60.000 60 24 0 237 296 359 418 1.85e-15 77.0
MS.gene51703.t1 AT4G34000 34.197 193 78 5 19 171 26 209 2.41e-21 94.4
MS.gene51703.t1 AT4G34000 60.000 65 25 1 237 301 359 422 2.43e-15 76.6
MS.gene51703.t1 AT4G34000 34.197 193 78 5 19 171 26 209 2.41e-21 94.4
MS.gene51703.t1 AT4G34000 60.000 65 25 1 237 301 359 422 2.43e-15 76.6
MS.gene51703.t1 AT4G34000 34.197 193 78 5 19 171 26 209 2.41e-21 94.4
MS.gene51703.t1 AT4G34000 60.000 65 25 1 237 301 359 422 2.43e-15 76.6
MS.gene51703.t1 AT1G45249 41.791 134 60 3 20 138 21 151 3.10e-21 93.2
MS.gene51703.t1 AT1G45249 41.791 134 60 3 20 138 21 151 3.10e-21 93.2
MS.gene51703.t1 AT1G45249 41.791 134 60 3 20 138 21 151 5.42e-21 93.2
MS.gene51703.t1 AT1G45249 41.791 134 60 3 20 138 21 151 5.42e-21 93.2
MS.gene51703.t1 AT1G45249 41.791 134 60 3 20 138 21 151 5.42e-21 93.2
MS.gene51703.t1 AT1G45249 41.791 134 60 3 20 138 21 151 5.42e-21 93.2
MS.gene51703.t1 AT1G45249 41.791 134 60 3 20 138 21 151 5.48e-21 93.2
MS.gene51703.t1 AT1G45249 48.958 96 30 3 237 320 326 414 1.70e-17 83.2
MS.gene51703.t1 AT1G45249 41.791 134 60 3 20 138 36 166 5.71e-21 93.2
MS.gene51703.t1 AT1G45249 48.958 96 30 3 237 320 341 429 2.26e-17 82.8
MS.gene51703.t1 AT1G45249 41.791 134 60 3 20 138 36 166 5.71e-21 93.2
MS.gene51703.t1 AT1G45249 48.958 96 30 3 237 320 341 429 2.26e-17 82.8
MS.gene51703.t1 AT1G45249 41.791 134 60 3 20 138 21 151 6.25e-21 93.2
MS.gene51703.t1 AT1G45249 43.925 107 30 4 237 320 326 425 1.16e-14 74.7
MS.gene51703.t1 AT4G35900 51.163 86 40 2 237 322 202 285 2.16e-16 78.6
MS.gene51703.t1 AT5G44080 30.882 204 94 6 96 297 120 278 5.90e-15 74.7
MS.gene51703.t1 AT2G17770 48.837 86 42 2 237 322 112 195 2.66e-14 70.9
MS.gene51703.t1 AT1G03970 31.373 204 84 6 96 297 85 234 3.34e-14 72.0

Find 69 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGCGACGAAGCTGGCATTT+AGG 0.252772 3.4:+6693037 None:intergenic
AGAAGACGGTTGATGAAGTT+TGG 0.321664 3.4:-6694744 MS.gene51703:CDS
GATTCAACAACACTGACTTT+AGG 0.341594 3.4:+6694571 None:intergenic
GCCACCTGACCATCTGTATA+TGG 0.350922 3.4:+6694388 None:intergenic
TTTGGGTGAGATGACATTAG+AGG 0.364587 3.4:-6694662 MS.gene51703:CDS
TCAACCGTCTTCTTGCTTAA+CGG 0.378968 3.4:+6694754 None:intergenic
CACAGAAATCTGCGAGAAAA+CGG 0.396824 3.4:-6694330 MS.gene51703:CDS
GGTGAAAGCAGGCGTTGTTT+CGG 0.397022 3.4:-6694632 MS.gene51703:CDS
CGAAGCTGGCATTTAGGTTC+AGG 0.401882 3.4:+6693043 None:intergenic
CAACCTAATCTGTCGTTAAC+CGG 0.406252 3.4:-6694778 MS.gene51703:CDS
ACAGAAATCTGCGAGAAAAC+GGG 0.427381 3.4:-6694329 MS.gene51703:CDS
CAGAATCACTTAGGTGACTT+AGG 0.427650 3.4:-6694925 MS.gene51703:CDS
TCAAATAGAAAGAATACTAA+TGG 0.443190 3.4:-6694601 MS.gene51703:CDS
CAAGAGATACAGTCTACTTT+GGG 0.443357 3.4:-6694679 MS.gene51703:CDS
TCAAGGTTCATACTACTTAG+TGG 0.444599 3.4:+6694898 None:intergenic
GCATTGGCTTCAACAGTGAT+AGG 0.448053 3.4:-6694367 MS.gene51703:CDS
CCAGAGGCTGGAATTGCGGT+TGG 0.448828 3.4:+6694989 None:intergenic
TCAAGAGATACAGTCTACTT+TGG 0.452722 3.4:-6694680 MS.gene51703:CDS
CGCGCCAGAGGCTGGAATTG+CGG 0.479085 3.4:+6694985 None:intergenic
TACAGATGGTCAGGTGGCAT+TGG 0.479585 3.4:-6694383 MS.gene51703:CDS
GGACCGGTTAACGACAGATT+AGG 0.482657 3.4:+6694775 None:intergenic
GCGCTCAAGAGCAAGGAAAC+AGG 0.483729 3.4:-6694233 MS.gene51703:intron
ATCTGCGAGAAAACGGGGAC+CGG 0.499262 3.4:-6694323 MS.gene51703:CDS
CCAACCGCAATTCCAGCCTC+TGG 0.504611 3.4:-6694989 MS.gene51703:CDS
GATGAAGTTCAGAATCACTT+AGG 0.504785 3.4:-6694934 MS.gene51703:CDS
TCTGAACTTCATCAAGGGTA+AGG 0.505070 3.4:+6694944 None:intergenic
GTCCTCTCAATCATGTCCTC+CGG 0.505376 3.4:+6694304 None:intergenic
CAGTCACTGCACATGACAGC+CGG 0.507159 3.4:-6694430 MS.gene51703:CDS
CGTCTTCTTGCTTAACGGAC+CGG 0.507684 3.4:+6694759 None:intergenic
GGAGATCAAAGTCTCACGTC+TGG 0.515777 3.4:-6694086 MS.gene51703:CDS
CAAAGTCTCACGTCTGGAAG+AGG 0.522058 3.4:-6694080 MS.gene51703:CDS
CCAGCTTCGTCGCGTATCAT+CGG 0.523412 3.4:-6693029 MS.gene51703:CDS
TGTTCCATATACAGATGGTC+AGG 0.524137 3.4:-6694392 MS.gene51703:CDS
TGGCATTTAGGTTCAGGCGG+CGG 0.526019 3.4:+6693049 None:intergenic
CTGTTGAGAGGAAGCAGAAG+AGG 0.527853 3.4:-6694282 MS.gene51703:CDS
GCAGTGACTGCGCTATGATC+TGG 0.531003 3.4:+6694443 None:intergenic
ATTCTTTCTATTTGAAGATG+CGG 0.537311 3.4:+6694609 None:intergenic
AACGAGGACACAGCGCAAGC+CGG 0.540740 3.4:-6694820 MS.gene51703:CDS
GACTGCGCTATGATCTGGCT+CGG 0.540969 3.4:+6694448 None:intergenic
AGTGATTCTGAACTTCATCA+AGG 0.543380 3.4:+6694938 None:intergenic
CTGCTGCGCGCTCAAGAGCA+AGG 0.547968 3.4:-6694240 MS.gene51703:CDS
TTAGGCATATACAACTGAGT+TGG 0.551690 3.4:-6694107 MS.gene51703:intron
CGCAATTCCAGCCTCTGGCG+CGG 0.552340 3.4:-6694984 MS.gene51703:CDS
GTCGCCATCACACTTTGTTG+TGG 0.565829 3.4:+6694478 None:intergenic
GGCAAACCAATCAATTCGCA+TGG 0.566719 3.4:-6694845 MS.gene51703:CDS
CCGATGATACGCGACGAAGC+TGG 0.578620 3.4:+6693029 None:intergenic
TGAAAAGCTGAGGAAAGAGA+AGG 0.578797 3.4:-6694053 MS.gene51703:intron
GTATATGGAACACCGTCTGA+TGG 0.581646 3.4:+6694403 None:intergenic
TGTTGTGGATGCTGATAGTG+TGG 0.595475 3.4:+6694493 None:intergenic
AACACCGTCTGATGGAGCAC+CGG 0.598081 3.4:+6694411 None:intergenic
TACTGTATCCACGGACCATG+TGG 0.602459 3.4:+6694523 None:intergenic
AGCTGGCATTTAGGTTCAGG+CGG 0.604208 3.4:+6693046 None:intergenic
ATCAATTCGCATGGAAAACG+AGG 0.609101 3.4:-6694836 MS.gene51703:CDS
GTGATTCTGAACTTCATCAA+GGG 0.610620 3.4:+6694939 None:intergenic
GCATCCACAACAAAGTGTGA+TGG 0.617192 3.4:-6694482 MS.gene51703:CDS
TGCGAGAAAACGGGGACCGG+AGG 0.618027 3.4:-6694320 MS.gene51703:CDS
GACCGGAGGACATGATTGAG+AGG 0.620098 3.4:-6694306 MS.gene51703:CDS
ACAACACTGACTTTAGGAGT+AGG 0.622952 3.4:+6694577 None:intergenic
GACGGTGTTCCATATACAGA+TGG 0.623089 3.4:-6694397 MS.gene51703:CDS
ACAGCCGGTGCTCCATCAGA+CGG 0.626618 3.4:-6694415 MS.gene51703:CDS
AAGAGGAAAATGAAAAGCTG+AGG 0.635284 3.4:-6694063 MS.gene51703:CDS
CAGAAATCTGCGAGAAAACG+GGG 0.637882 3.4:-6694328 MS.gene51703:CDS
GGTTGTGCATACTGTATCCA+CGG 0.641304 3.4:+6694514 None:intergenic
TGATTGAGAGGACTGTTGAG+AGG 0.642725 3.4:-6694294 MS.gene51703:CDS
CCAACAAAACAACGATCATG+AGG 0.650306 3.4:-6694713 MS.gene51703:CDS
TCCATATACAGATGGTCAGG+TGG 0.655838 3.4:-6694389 MS.gene51703:CDS
CGGTCCGTTAAGCAAGAAGA+CGG 0.656636 3.4:-6694758 MS.gene51703:CDS
ACAAAACAACGATCATGAGG+AGG 0.694318 3.4:-6694710 MS.gene51703:CDS
GGACCATGTGGAGAAAACTG+TGG 0.769588 3.4:+6694535 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAGAATTTCAAAAACA+TGG + chr3.4:6693864-6693883 None:intergenic 15.0%
!! ATTTACATTCTTAATTCTTA+TGG - chr3.4:6694158-6694177 MS.gene51703:intron 15.0%
!!! ATGTTAGAAATTCAAATTTT+TGG - chr3.4:6694866-6694885 MS.gene51703:CDS 15.0%
!! AATACATATGAAGGAATAAA+AGG + chr3.4:6694424-6694443 None:intergenic 20.0%
!! AGATATTGAAATCAGTAAAT+TGG + chr3.4:6694671-6694690 None:intergenic 20.0%
!! TACTTAATATCAAGAAACTA+AGG + chr3.4:6694099-6694118 None:intergenic 20.0%
!! TCAAATAGAAAGAATACTAA+TGG - chr3.4:6693444-6693463 MS.gene51703:intron 20.0%
!!! ATTAACAGAAACATTTTCAA+TGG + chr3.4:6694049-6694068 None:intergenic 20.0%
!!! GATTTCAATATCTAAACTTT+TGG - chr3.4:6694678-6694697 MS.gene51703:CDS 20.0%
!!! TTGTTGCATTGTAATTTTTT+TGG - chr3.4:6694807-6694826 MS.gene51703:CDS 20.0%
! AATGAGTAAAAAGATCATGA+AGG - chr3.4:6694363-6694382 MS.gene51703:CDS 25.0%
! ATTCTTTCTATTTGAAGATG+CGG + chr3.4:6693439-6693458 None:intergenic 25.0%
! GCAACAAGATTAATAAACAA+AGG + chr3.4:6694794-6694813 None:intergenic 25.0%
! GTGTTTATTAACAACACATA+AGG + chr3.4:6694742-6694761 None:intergenic 25.0%
! TCTTAATTCTTATGGTGTTT+GGG - chr3.4:6694166-6694185 MS.gene51703:intron 25.0%
! TTCTTAATTCTTATGGTGTT+TGG - chr3.4:6694165-6694184 MS.gene51703:intron 25.0%
! TTGGAATGAATGTATCTTTA+TGG - chr3.4:6694885-6694904 MS.gene51703:CDS 25.0%
! TTTCTCATACTTCAAATTCA+AGG - chr3.4:6694500-6694519 MS.gene51703:CDS 25.0%
!! ATCTCATTTAAGTTTCTGTA+CGG - chr3.4:6694121-6694140 MS.gene51703:intron 25.0%
!! TTACTGCTTAGAATTTTCAT+TGG - chr3.4:6694225-6694244 MS.gene51703:intron 25.0%
!!! CATTGTAATTTTTTTGGAAG+AGG - chr3.4:6694813-6694832 MS.gene51703:CDS 25.0%
AGTGTGAGAAATACATATGA+AGG + chr3.4:6694433-6694452 None:intergenic 30.0%
ATGGAAAAAGTTGTTTACTC+GGG + chr3.4:6693903-6693922 None:intergenic 30.0%
CACAATTAGTACTCATGTAT+TGG - chr3.4:6694332-6694351 MS.gene51703:CDS 30.0%
CATGGAAAAAGTTGTTTACT+CGG + chr3.4:6693904-6693923 None:intergenic 30.0%
CTAATTACAGTTACAATCCA+TGG + chr3.4:6693922-6693941 None:intergenic 30.0%
GGTTGATTGTACTATATACA+AGG + chr3.4:6694721-6694740 None:intergenic 30.0%
TTTCTCACACTTCAAATTCA+AGG - chr3.4:6694442-6694461 MS.gene51703:CDS 30.0%
! ATTAGTACTCATGTATTGGT+TGG - chr3.4:6694336-6694355 MS.gene51703:CDS 30.0%
! CATGGATTGTAACTGTAATT+AGG - chr3.4:6693920-6693939 MS.gene51703:intron 30.0%
! TTCTACTTGTTGACTGTAAT+TGG - chr3.4:6694386-6694405 MS.gene51703:CDS 30.0%
!!! GATGACATTAGAGGATTTTT+TGG - chr3.4:6693392-6693411 MS.gene51703:intron 30.0%
AAGAGGAAAATGAAAAGCTG+AGG - chr3.4:6693982-6694001 MS.gene51703:intron 35.0%
AAGTAAGCACAAGACAGTAA+AGG + chr3.4:6694267-6694286 None:intergenic 35.0%
ACGTTGAAAAACAACTTGTC+CGG + chr3.4:6693247-6693266 None:intergenic 35.0%
AGTAAGCACAAGACAGTAAA+GGG + chr3.4:6694266-6694285 None:intergenic 35.0%
AGTGATTCTGAACTTCATCA+AGG + chr3.4:6693110-6693129 None:intergenic 35.0%
CAAGAGATACAGTCTACTTT+GGG - chr3.4:6693366-6693385 MS.gene51703:intron 35.0%
CGTTGCATAATGTATTTGGA+TGG - chr3.4:6694838-6694857 MS.gene51703:CDS 35.0%
GAAATTGTGCACTTTGATGT+TGG - chr3.4:6694188-6694207 MS.gene51703:intron 35.0%
GATGAAGTTCAGAATCACTT+AGG - chr3.4:6693111-6693130 MS.gene51703:intron 35.0%
GTAAAGGGTCACATATTCTA+TGG + chr3.4:6694251-6694270 None:intergenic 35.0%
GTGATTCTGAACTTCATCAA+GGG + chr3.4:6693109-6693128 None:intergenic 35.0%
TCAAGAGATACAGTCTACTT+TGG - chr3.4:6693365-6693384 MS.gene51703:intron 35.0%
TCAAGGTTCATACTACTTAG+TGG + chr3.4:6693150-6693169 None:intergenic 35.0%
TTAGGCATATACAACTGAGT+TGG - chr3.4:6693938-6693957 MS.gene51703:intron 35.0%
TTGTCTATGTGTCAATATCG+TGG - chr3.4:6694603-6694622 MS.gene51703:CDS 35.0%
! ACGAGCTTCTGAAAAATGTA+TGG - chr3.4:6693169-6693188 MS.gene51703:intron 35.0%
! GAAAAATGTATGGACTGTTG+AGG - chr3.4:6693179-6693198 MS.gene51703:intron 35.0%
! GTTTTACTTACTCAACTTGC+AGG - chr3.4:6694947-6694966 MS.gene51703:CDS 35.0%
!! ACTGTCTTGTGCTTACTTTT+AGG - chr3.4:6694269-6694288 MS.gene51703:CDS 35.0%
!! CGAGTAAACAACTTTTTCCA+TGG - chr3.4:6693902-6693921 MS.gene51703:intron 35.0%
!! GATTCAACAACACTGACTTT+AGG + chr3.4:6693477-6693496 None:intergenic 35.0%
ACAAAACAACGATCATGAGG+AGG - chr3.4:6693335-6693354 MS.gene51703:intron 40.0%
ACAGAAATCTGCGAGAAAAC+GGG - chr3.4:6693716-6693735 MS.gene51703:intron 40.0%
AGTTGTTTACTCGGGAGAAA+TGG + chr3.4:6693895-6693914 None:intergenic 40.0%
ATCAATTCGCATGGAAAACG+AGG - chr3.4:6693209-6693228 MS.gene51703:intron 40.0%
CAACCTAATCTGTCGTTAAC+CGG - chr3.4:6693267-6693286 MS.gene51703:intron 40.0%
CACAGAAATCTGCGAGAAAA+CGG - chr3.4:6693715-6693734 MS.gene51703:intron 40.0%
CAGAATCACTTAGGTGACTT+AGG - chr3.4:6693120-6693139 MS.gene51703:intron 40.0%
CCAACAAAACAACGATCATG+AGG - chr3.4:6693332-6693351 MS.gene51703:intron 40.0%
CTTCAAATTCAAGGCGTGTT+TGG - chr3.4:6694451-6694470 MS.gene51703:CDS 40.0%
GGCACGTTGCATAATGTATT+TGG - chr3.4:6694834-6694853 MS.gene51703:CDS 40.0%
TCAACCGTCTTCTTGCTTAA+CGG + chr3.4:6693294-6693313 None:intergenic 40.0%
TCTGAACTTCATCAAGGGTA+AGG + chr3.4:6693104-6693123 None:intergenic 40.0%
TGAAAAGCTGAGGAAAGAGA+AGG - chr3.4:6693992-6694011 MS.gene51703:intron 40.0%
TGTTCCATATACAGATGGTC+AGG - chr3.4:6693653-6693672 MS.gene51703:intron 40.0%
TTTGGGTGAGATGACATTAG+AGG - chr3.4:6693383-6693402 MS.gene51703:intron 40.0%
! CGTTTTCCATGCGAATTGAT+TGG + chr3.4:6693209-6693228 None:intergenic 40.0%
!! ACAACACTGACTTTAGGAGT+AGG + chr3.4:6693471-6693490 None:intergenic 40.0%
!! AGAAGACGGTTGATGAAGTT+TGG - chr3.4:6693301-6693320 MS.gene51703:intron 40.0%
!! CATTTTTCAGAAGCTCGTCA+AGG + chr3.4:6693167-6693186 None:intergenic 40.0%
!! CCTCATGATCGTTGTTTTGT+TGG + chr3.4:6693335-6693354 None:intergenic 40.0%
!!! GAGGATTTTTTGGTGAAAGC+AGG - chr3.4:6693402-6693421 MS.gene51703:intron 40.0%
CAGAAATCTGCGAGAAAACG+GGG - chr3.4:6693717-6693736 MS.gene51703:intron 45.0%
GACGGTGTTCCATATACAGA+TGG - chr3.4:6693648-6693667 MS.gene51703:intron 45.0%
GCATCCACAACAAAGTGTGA+TGG - chr3.4:6693563-6693582 MS.gene51703:intron 45.0%
GGCAAACCAATCAATTCGCA+TGG - chr3.4:6693200-6693219 MS.gene51703:intron 45.0%
GGTTGTGCATACTGTATCCA+CGG + chr3.4:6693534-6693553 None:intergenic 45.0%
GTATATGGAACACCGTCTGA+TGG + chr3.4:6693645-6693664 None:intergenic 45.0%
TCCATATACAGATGGTCAGG+TGG - chr3.4:6693656-6693675 MS.gene51703:intron 45.0%
TGATTGAGAGGACTGTTGAG+AGG - chr3.4:6693751-6693770 MS.gene51703:intron 45.0%
TGTCTCTGTCAACGCTTCAT+AGG - chr3.4:6694629-6694648 MS.gene51703:CDS 45.0%
! TGTTGTGGATGCTGATAGTG+TGG + chr3.4:6693555-6693574 None:intergenic 45.0%
! TTGCCACAGTTTTCTCCACA+TGG - chr3.4:6693507-6693526 MS.gene51703:intron 45.0%
!! ATGAAGCGTTGACAGAGACA+CGG + chr3.4:6694629-6694648 None:intergenic 45.0%
!! GCATTGGCTTCAACAGTGAT+AGG - chr3.4:6693678-6693697 MS.gene51703:intron 45.0%
CAAAGTCTCACGTCTGGAAG+AGG - chr3.4:6693965-6693984 MS.gene51703:intron 50.0%
CGGTCCGTTAAGCAAGAAGA+CGG - chr3.4:6693287-6693306 MS.gene51703:intron 50.0%
CTGTTGAGAGGAAGCAGAAG+AGG - chr3.4:6693763-6693782 MS.gene51703:intron 50.0%
GCCACCTGACCATCTGTATA+TGG + chr3.4:6693660-6693679 None:intergenic 50.0%
GGACCATGTGGAGAAAACTG+TGG + chr3.4:6693513-6693532 None:intergenic 50.0%
GGACCGGTTAACGACAGATT+AGG + chr3.4:6693273-6693292 None:intergenic 50.0%
GGAGATCAAAGTCTCACGTC+TGG - chr3.4:6693959-6693978 MS.gene51703:intron 50.0%
GTCCTCTCAATCATGTCCTC+CGG + chr3.4:6693744-6693763 None:intergenic 50.0%
GTCGCCATCACACTTTGTTG+TGG + chr3.4:6693570-6693589 None:intergenic 50.0%
TACAGATGGTCAGGTGGCAT+TGG - chr3.4:6693662-6693681 MS.gene51703:intron 50.0%
TACTGTATCCACGGACCATG+TGG + chr3.4:6693525-6693544 None:intergenic 50.0%
! AGCTGGCATTTAGGTTCAGG+CGG + chr3.4:6695002-6695021 None:intergenic 50.0%
! AGTTTTCTCCACATGGTCCG+TGG - chr3.4:6693514-6693533 MS.gene51703:intron 50.0%
! ATTGAATTTTGCCGCGCCAG+AGG + chr3.4:6693075-6693094 None:intergenic 50.0%
! CGAAGCTGGCATTTAGGTTC+AGG + chr3.4:6695005-6695024 None:intergenic 50.0%
! CGTCTTCTTGCTTAACGGAC+CGG + chr3.4:6693289-6693308 None:intergenic 50.0%
! GGTGAAAGCAGGCGTTGTTT+CGG - chr3.4:6693413-6693432 MS.gene51703:intron 50.0%
AACACCGTCTGATGGAGCAC+CGG + chr3.4:6693637-6693656 None:intergenic 55.0%
ACGCGACGAAGCTGGCATTT+AGG + chr3.4:6695011-6695030 None:intergenic 55.0%
ATCTGCGAGAAAACGGGGAC+CGG - chr3.4:6693722-6693741 MS.gene51703:intron 55.0%
CAGTCACTGCACATGACAGC+CGG - chr3.4:6693615-6693634 MS.gene51703:intron 55.0%
CCAGCTTCGTCGCGTATCAT+CGG - chr3.4:6695016-6695035 MS.gene51703:CDS 55.0%
GACCGGAGGACATGATTGAG+AGG - chr3.4:6693739-6693758 MS.gene51703:intron 55.0%
GACTGCGCTATGATCTGGCT+CGG + chr3.4:6693600-6693619 None:intergenic 55.0%
GCAGTGACTGCGCTATGATC+TGG + chr3.4:6693605-6693624 None:intergenic 55.0%
GCGCTCAAGAGCAAGGAAAC+AGG - chr3.4:6693812-6693831 MS.gene51703:intron 55.0%
! TGGCATTTAGGTTCAGGCGG+CGG + chr3.4:6694999-6695018 None:intergenic 55.0%
AACGAGGACACAGCGCAAGC+CGG - chr3.4:6693225-6693244 MS.gene51703:intron 60.0%
ACAGCCGGTGCTCCATCAGA+CGG - chr3.4:6693630-6693649 MS.gene51703:intron 60.0%
CCAACCGCAATTCCAGCCTC+TGG - chr3.4:6693056-6693075 MS.gene51703:CDS 60.0%
CCAGAGGCTGGAATTGCGGT+TGG + chr3.4:6693059-6693078 None:intergenic 60.0%
CCGATGATACGCGACGAAGC+TGG + chr3.4:6695019-6695038 None:intergenic 60.0%
! AATTTTGCCGCGCCAGAGGC+TGG + chr3.4:6693071-6693090 None:intergenic 60.0%
CGCAATTCCAGCCTCTGGCG+CGG - chr3.4:6693061-6693080 MS.gene51703:CDS 65.0%
CGCGCCAGAGGCTGGAATTG+CGG + chr3.4:6693063-6693082 None:intergenic 65.0%
CTGCTGCGCGCTCAAGAGCA+AGG - chr3.4:6693805-6693824 MS.gene51703:intron 65.0%
TGCGAGAAAACGGGGACCGG+AGG - chr3.4:6693725-6693744 MS.gene51703:intron 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 6693018 6695049 6693018 ID=MS.gene51703
chr3.4 mRNA 6693018 6695049 6693018 ID=MS.gene51703.t1;Parent=MS.gene51703
chr3.4 exon 6694234 6695049 6694234 ID=MS.gene51703.t1.exon1;Parent=MS.gene51703.t1
chr3.4 CDS 6694234 6695049 6694234 ID=cds.MS.gene51703.t1;Parent=MS.gene51703.t1
chr3.4 exon 6694054 6694125 6694054 ID=MS.gene51703.t1.exon2;Parent=MS.gene51703.t1
chr3.4 CDS 6694054 6694125 6694054 ID=cds.MS.gene51703.t1;Parent=MS.gene51703.t1
chr3.4 exon 6693018 6693098 6693018 ID=MS.gene51703.t1.exon3;Parent=MS.gene51703.t1
chr3.4 CDS 6693018 6693098 6693018 ID=cds.MS.gene51703.t1;Parent=MS.gene51703.t1
Gene Sequence

>MS.gene51703

ATGGGGTCTCAAGTTGTTGATGAAAGTAATAATGAAAACCAACCGCAATTCCAGCCTCTGGCGCGGCAAAATTCAATGTACAACCTTACCCTTGATGAAGTTCAGAATCACTTAGGTGACTTAGGAAAGCCACTAAGTAGTATGAACCTTGACGAGCTTCTGAAAAATGTATGGACTGTTGAGGCAAACCAATCAATTCGCATGGAAAACGAGGACACAGCGCAAGCCGGACAAGTTGTTTTTCAACGTCAACCTAATCTGTCGTTAACCGGTCCGTTAAGCAAGAAGACGGTTGATGAAGTTTGGAGAGATATCCAACAAAACAACGATCATGAGGAGGTGAAGTCTCAAGAGATACAGTCTACTTTGGGTGAGATGACATTAGAGGATTTTTTGGTGAAAGCAGGCGTTGTTTCGGCCGCATCTTCAAATAGAAAGAATACTAATGGTCCTACTCCTAAAGTCAGTGTTGTTGAATCGAATGTAGCGTTGCCACAGTTTTCTCCACATGGTCCGTGGATACAGTATGCACAACCACACTATCAGCATCCACAACAAAGTGTGATGGCGACTTATGTTCCGAGCCAGATCATAGCGCAGTCACTGCACATGACAGCCGGTGCTCCATCAGACGGTGTTCCATATACAGATGGTCAGGTGGCATTGGCTTCAACAGTGATAGGAAACTTATCTGATACACAGAAATCTGCGAGAAAACGGGGACCGGAGGACATGATTGAGAGGACTGTTGAGAGGAAGCAGAAGAGGATGATTAAGAATCGAGAATCTGCTGCGCGCTCAAGAGCAAGGAAACAGGTGATTTTTTTCTTTTTCTTTTGAATGCCATGTTTTTGAAATTCTAATTTTTTATCATCCATTTCTCCCGAGTAAACAACTTTTTCCATGGATTGTAACTGTAATTAGGCATATACAACTGAGTTGGAGATCAAAGTCTCACGTCTGGAAGAGGAAAATGAAAAGCTGAGGAAAGAGAAGGTTAGATTTTGCTTAGAAGTTATTGTTTGAATCCATTGAAAATGTTTCTGTTAATTCAATTTGCTGAATAATTTGCTCAATACCTTAGTTTCTTGATATTAAGTAATATCTCATTTAAGTTTCTGTACGGACATTAATTTATTTATTTACATTCTTAATTCTTATGGTGTTTGGGAAATTGTGCACTTTGATGTTGGTCTTGAATTATCAGTTACTGCTTAGAATTTTCATTGGCCATAGAATATGTGACCCTTTACTGTCTTGTGCTTACTTTTAGGCTTACAATTCATAGTTGCGATAAGAATATTTTATTTCTTACACAATTAGTACTCATGTATTGGTTGGAAGTAATGAGTAAAAAGATCATGAAGGTTCTACTTGTTGACTGTAATTGGTTGTTATTATTTCCTTTTATTCCTTCATATGTATTTCTCACACTTCAAATTCAAGGCGTGTTTGGTGTCTGACAATGATATGATATTGACATTTCTCATACTTCAAATTCAAGGCGTGTTTGGTGTCTGACAATGATATGATATTGACACATTTGATTATATTTAATCACTTTCGTTTTCTGAAATTATTACTATTGTCTATGTGTCAATATCGTGGCCGTGTCTCTGTCAACGCTTCATAGGATAAGATTATCATTTACCAATTTACTGATTTCAATATCTAAACTTTTGGATTGTTGATTCTATTCTCCTTGTATATAGTACAATCAACCTTATGTGTTGTTAATAAACACAATATAGACTTGTGTATTCAGCTTCAAAACCTTTGTTTATTAATCTTGTTGCATTGTAATTTTTTTGGAAGAGGCACGTTGCATAATGTATTTGGATGGTGTCAATGTTAGAAATTCAAATTTTTGGAATGAATGTATCTTTATGGTCTTCGATGCATCTTTTCTTACTTGTTCTTCGTTAATTTGTTTTACTTACTCAACTTGCAGGAGCTTGAGAACATGCTAGCTAATGCTCCGCCGCCTGAACCTAAATGCCAGCTTCGTCGCGTATCATCGGCTTCATTTTGA
Protein sequence

>MS.gene51703.t1

MGSQVVDESNNENQPQFQPLARQNSMYNLTLDEVQNHLGDLGKPLSSMNLDELLKNVWTVEANQSIRMENEDTAQAGQVVFQRQPNLSLTGPLSKKTVDEVWRDIQQNNDHEEVKSQEIQSTLGEMTLEDFLVKAGVVSAASSNRKNTNGPTPKVSVVESNVALPQFSPHGPWIQYAQPHYQHPQQSVMATYVPSQIIAQSLHMTAGAPSDGVPYTDGQVALASTVIGNLSDTQKSARKRGPEDMIERTVERKQKRMIKNRESAARSRARKQAYTTELEIKVSRLEEENEKLRKEKELENMLANAPPPEPKCQLRRVSSASF