Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51726.t1 | XP_004508645.1 | 100 | 46 | 0 | 0 | 1 | 46 | 290 | 335 | 6.50E-18 | 99 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51726.t1 | Q9LEZ3 | 80.4 | 46 | 9 | 0 | 1 | 46 | 298 | 343 | 2.9e-14 | 78.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51726.t1 | A0A072UQV4 | 100.0 | 46 | 0 | 0 | 1 | 46 | 156 | 201 | 4.7e-18 | 99.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene51726.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51726.t1 | MTR_4g110040 | 100.000 | 46 | 0 | 0 | 1 | 46 | 156 | 201 | 1.09e-27 | 102 |
| MS.gene51726.t1 | MTR_4g110040 | 100.000 | 46 | 0 | 0 | 1 | 46 | 288 | 333 | 1.67e-27 | 102 |
| MS.gene51726.t1 | MTR_5g048350 | 71.739 | 46 | 13 | 0 | 1 | 46 | 66 | 111 | 1.14e-19 | 79.3 |
| MS.gene51726.t1 | MTR_7g017320 | 73.333 | 45 | 12 | 0 | 2 | 46 | 73 | 117 | 3.47e-18 | 75.1 |
| MS.gene51726.t1 | MTR_7g017320 | 73.333 | 45 | 12 | 0 | 2 | 46 | 73 | 117 | 3.78e-18 | 74.7 |
| MS.gene51726.t1 | MTR_5g048350 | 58.696 | 46 | 10 | 1 | 1 | 46 | 66 | 102 | 7.97e-12 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51726.t1 | AT5G08130 | 80.435 | 46 | 9 | 0 | 1 | 46 | 178 | 223 | 1.96e-20 | 82.0 |
| MS.gene51726.t1 | AT5G08130 | 80.435 | 46 | 9 | 0 | 1 | 46 | 178 | 223 | 2.13e-20 | 81.6 |
| MS.gene51726.t1 | AT5G08130 | 80.435 | 46 | 9 | 0 | 1 | 46 | 298 | 343 | 6.23e-20 | 80.9 |
| MS.gene51726.t1 | AT5G08130 | 80.435 | 46 | 9 | 0 | 1 | 46 | 298 | 343 | 6.81e-20 | 80.5 |
| MS.gene51726.t1 | AT5G08130 | 80.435 | 46 | 9 | 0 | 1 | 46 | 298 | 343 | 6.81e-20 | 80.5 |
| MS.gene51726.t1 | AT5G08130 | 80.435 | 46 | 9 | 0 | 1 | 46 | 302 | 347 | 6.82e-20 | 80.5 |
| MS.gene51726.t1 | AT5G08130 | 80.435 | 46 | 9 | 0 | 1 | 46 | 302 | 347 | 7.44e-20 | 80.5 |
| MS.gene51726.t1 | AT5G08130 | 80.435 | 46 | 9 | 0 | 1 | 46 | 298 | 343 | 9.22e-20 | 80.1 |
| MS.gene51726.t1 | AT1G69010 | 71.739 | 46 | 13 | 0 | 1 | 46 | 67 | 112 | 1.18e-18 | 76.3 |
| MS.gene51726.t1 | AT5G38860 | 75.000 | 44 | 9 | 1 | 2 | 43 | 56 | 99 | 7.51e-15 | 65.1 |
| MS.gene51726.t1 | AT5G38860 | 75.000 | 44 | 9 | 1 | 2 | 43 | 56 | 99 | 8.30e-15 | 65.9 |
Find 10 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAATTTACCTCTAACAAAA+AGG | 0.343203 | 3.4:+7315769 | None:intergenic |
| GGCATTCGTTATTCCAACCT+TGG | 0.426366 | 3.4:+7315617 | None:intergenic |
| ATGAAGGTTCATTCCAAGGT+TGG | 0.500331 | 3.4:-7315630 | MS.gene51726:CDS |
| TTCCACACAGTGATCAAAAG+AGG | 0.540612 | 3.4:-7315802 | MS.gene51726:CDS |
| GAGAAGGTTCATAAGTATGA+AGG | 0.541707 | 3.4:-7315646 | MS.gene51726:CDS |
| AAGTATGAAGGTTCATTCCA+AGG | 0.551145 | 3.4:-7315634 | MS.gene51726:CDS |
| GGTTGGAATAACGAATGCCA+TGG | 0.585339 | 3.4:-7315613 | MS.gene51726:CDS |
| GAAAGTGTGGTATTTACCCA+TGG | 0.625507 | 3.4:+7315596 | None:intergenic |
| GTTGGAATAACGAATGCCAT+GGG | 0.643560 | 3.4:-7315612 | MS.gene51726:CDS |
| TCCACACAGTGATCAAAAGA+GGG | 0.647542 | 3.4:-7315801 | MS.gene51726:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTTAATCTAAATTATTTGT+AGG | - | chr3.4:7315732-7315751 | MS.gene51726:intron | 15.0% |
| ! | TGAATTTACCTCTAACAAAA+AGG | + | chr3.4:7315661-7315680 | None:intergenic | 25.0% |
| ! | TTGTTAGAGGTAAATTCATA+CGG | - | chr3.4:7315663-7315682 | MS.gene51726:CDS | 25.0% |
| !!! | CATTCACTTTTTACAAGAGA+AGG | - | chr3.4:7315765-7315784 | MS.gene51726:intron | 30.0% |
| AAGTATGAAGGTTCATTCCA+AGG | - | chr3.4:7315793-7315812 | MS.gene51726:CDS | 35.0% | |
| GAGAAGGTTCATAAGTATGA+AGG | - | chr3.4:7315781-7315800 | MS.gene51726:CDS | 35.0% | |
| TAGAGGTAAATTCATACGGT+TGG | - | chr3.4:7315667-7315686 | MS.gene51726:CDS | 35.0% | |
| !! | CAAAGCATCCTTTTTGTTAG+AGG | - | chr3.4:7315650-7315669 | MS.gene51726:CDS | 35.0% |
| ATGAAGGTTCATTCCAAGGT+TGG | - | chr3.4:7315797-7315816 | MS.gene51726:CDS | 40.0% | |
| GTTGGAATAACGAATGCCAT+GGG | - | chr3.4:7315815-7315834 | MS.gene51726:CDS | 40.0% | |
| TCCACACAGTGATCAAAAGA+GGG | - | chr3.4:7315626-7315645 | MS.gene51726:CDS | 40.0% | |
| TTCCACACAGTGATCAAAAG+AGG | - | chr3.4:7315625-7315644 | MS.gene51726:CDS | 40.0% | |
| GGCATTCGTTATTCCAACCT+TGG | + | chr3.4:7315813-7315832 | None:intergenic | 45.0% | |
| GGTTGGAATAACGAATGCCA+TGG | - | chr3.4:7315814-7315833 | MS.gene51726:CDS | 45.0% | |
| ! | TCCCTCTTTTGATCACTGTG+TGG | + | chr3.4:7315630-7315649 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 7315609 | 7315840 | 7315609 | ID=MS.gene51726 |
| chr3.4 | mRNA | 7315609 | 7315840 | 7315609 | ID=MS.gene51726.t1;Parent=MS.gene51726 |
| chr3.4 | exon | 7315778 | 7315840 | 7315778 | ID=MS.gene51726.t1.exon1;Parent=MS.gene51726.t1 |
| chr3.4 | CDS | 7315778 | 7315840 | 7315778 | ID=cds.MS.gene51726.t1;Parent=MS.gene51726.t1 |
| chr3.4 | exon | 7315609 | 7315695 | 7315609 | ID=MS.gene51726.t1.exon2;Parent=MS.gene51726.t1 |
| chr3.4 | CDS | 7315609 | 7315695 | 7315609 | ID=cds.MS.gene51726.t1;Parent=MS.gene51726.t1 |
| Gene Sequence |
| Protein sequence |
