AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52161

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52161.t1 MTR_7g076080 97.943 778 16 0 1 778 1 778 0.0 1580
MS.gene52161.t1 MTR_8g432540 80.454 793 128 11 1 778 30 810 0.0 1273
MS.gene52161.t1 MTR_1g101280 64.085 749 231 9 53 775 15 751 0.0 948
MS.gene52161.t1 MTR_4g047800 53.887 759 313 13 30 778 2 733 0.0 778
MS.gene52161.t1 MTR_2g088470 54.654 752 306 14 37 777 8 735 0.0 771
MS.gene52161.t1 MTR_0198s0030 51.963 764 313 16 44 774 9 751 0.0 768
MS.gene52161.t1 MTR_6g477820 48.883 716 321 12 91 778 29 727 0.0 661
MS.gene52161.t1 MTR_2g101720 43.333 690 344 10 95 777 143 792 0.0 601
MS.gene52161.t1 MTR_5g029320 43.276 751 371 18 55 774 52 778 0.0 601
MS.gene52161.t1 MTR_5g005600 43.011 744 372 16 46 774 10 716 0.0 544
MS.gene52161.t1 MTR_7g427710 42.616 711 309 17 85 776 10 640 2.30e-176 521
MS.gene52161.t1 MTR_2g030570 34.545 715 438 13 67 774 4 695 1.65e-134 415
MS.gene52161.t1 MTR_2g030600 33.801 713 439 14 67 774 4 688 9.96e-127 394
MS.gene52161.t1 MTR_2g030660 31.780 708 409 22 78 774 3 647 1.36e-98 319
MS.gene52161.t1 MTR_7g427690 43.077 390 194 8 285 666 57 426 1.97e-97 310
MS.gene52161.t1 MTR_2g083350 34.725 527 293 11 290 778 22 535 7.16e-88 288
MS.gene52161.t1 MTR_2g083330 32.824 524 304 8 290 778 19 529 1.80e-81 271
MS.gene52161.t1 MTR_7g427700 38.580 324 139 5 85 402 10 279 1.97e-62 216
MS.gene52161.t1 MTR_7g427700 44.444 162 71 5 625 776 281 433 1.75e-29 122
MS.gene52161.t1 MTR_0653s0020 40.376 213 108 5 468 678 1 196 1.62e-45 162
MS.gene52161.t1 MTR_5g046040 49.645 141 70 1 91 230 33 173 3.66e-40 152
MS.gene52161.t1 MTR_7g027265 55.000 120 54 0 91 210 17 136 9.64e-39 140
MS.gene52161.t1 MTR_2g030690 31.741 293 175 10 491 774 1 277 2.83e-28 115
MS.gene52161.t1 MTR_1g105420 31.925 213 108 6 146 344 1 190 3.32e-24 102
MS.gene52161.t1 MTR_0653s0010 49.593 123 45 4 664 776 1 116 3.18e-23 95.9
MS.gene52161.t1 MTR_2g451350 36.029 136 77 2 297 427 1 131 8.10e-22 92.4
MS.gene52161.t1 MTR_7g027605 49.462 93 44 2 373 465 2 91 2.47e-21 89.4
MS.gene52161.t1 MTR_6g027290 29.193 161 109 2 74 229 4 164 1.59e-18 84.3
MS.gene52161.t1 MTR_7g427670 42.222 90 40 2 145 233 32 110 6.13e-14 69.7
MS.gene52161.t1 MTR_2g451350 33.333 75 50 0 353 427 18 92 2.91e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52161.t1 AT4G00730 69.599 773 210 8 11 778 50 802 0.0 1061
MS.gene52161.t1 AT3G61150 64.838 802 230 13 1 778 34 807 0.0 1018
MS.gene52161.t1 AT4G04890 54.290 746 293 13 52 777 21 738 0.0 790
MS.gene52161.t1 AT4G04890 54.290 746 293 13 52 777 21 738 0.0 790
MS.gene52161.t1 AT4G21750 55.281 729 268 12 84 777 55 760 0.0 770
MS.gene52161.t1 AT4G21750 55.281 729 268 12 84 777 55 760 0.0 770
MS.gene52161.t1 AT4G21750 55.281 729 268 12 84 777 55 760 0.0 770
MS.gene52161.t1 AT4G21750 55.281 729 268 12 84 777 55 760 0.0 770
MS.gene52161.t1 AT1G05230 53.142 732 306 14 50 778 23 720 0.0 761
MS.gene52161.t1 AT1G05230 53.142 732 306 14 50 778 23 720 0.0 761
MS.gene52161.t1 AT1G05230 53.142 732 306 14 50 778 23 720 0.0 761
MS.gene52161.t1 AT1G05230 53.142 732 306 14 50 778 23 720 0.0 761
MS.gene52161.t1 AT1G05230 53.142 732 306 14 50 778 23 720 0.0 761
MS.gene52161.t1 AT1G05230 53.142 732 306 14 50 778 23 720 0.0 761
MS.gene52161.t1 AT1G05230 53.142 732 306 14 50 778 23 720 0.0 761
MS.gene52161.t1 AT1G05230 53.142 732 306 14 50 778 23 720 0.0 761
MS.gene52161.t1 AT1G05230 53.142 732 306 14 50 778 23 720 0.0 761
MS.gene52161.t1 AT1G05230 53.142 732 306 14 50 778 23 720 0.0 761
MS.gene52161.t1 AT1G05230 53.142 732 304 15 50 778 23 718 0.0 757
MS.gene52161.t1 AT5G52170 52.982 721 268 12 61 775 24 679 0.0 740
MS.gene52161.t1 AT5G52170 52.982 721 268 12 61 775 24 679 0.0 740
MS.gene52161.t1 AT5G52170 52.617 726 268 13 61 775 24 684 0.0 734
MS.gene52161.t1 AT4G00730 65.844 527 157 7 11 532 50 558 0.0 684
MS.gene52161.t1 AT1G73360 47.783 699 333 10 91 774 32 713 0.0 626
MS.gene52161.t1 AT1G17920 46.286 700 323 14 94 778 24 685 0.0 614
MS.gene52161.t1 AT1G17920 46.286 700 323 14 94 778 24 685 0.0 614
MS.gene52161.t1 AT1G05230 51.396 609 265 13 50 655 23 603 0.0 608
MS.gene52161.t1 AT2G32370 43.966 696 343 11 91 778 68 724 0.0 573
MS.gene52161.t1 AT1G79840 41.774 699 347 12 88 777 98 745 0.0 558
MS.gene52161.t1 AT1G79840 41.774 699 347 12 88 777 40 687 0.0 557
MS.gene52161.t1 AT1G79840 41.774 699 347 12 88 777 40 687 0.0 557
MS.gene52161.t1 AT1G79840 41.774 699 347 12 88 777 127 774 0.0 556
MS.gene52161.t1 AT5G46880 41.667 720 374 17 84 774 104 806 8.56e-175 523
MS.gene52161.t1 AT5G46880 41.667 720 374 17 84 774 104 806 8.56e-175 523
MS.gene52161.t1 AT5G46880 41.667 720 374 17 84 774 104 806 8.56e-175 523
MS.gene52161.t1 AT4G25530 39.636 714 368 16 80 778 21 686 4.40e-163 488
MS.gene52161.t1 AT1G79840 41.373 568 284 9 88 651 98 620 3.02e-153 461
MS.gene52161.t1 AT4G17710 40.115 698 328 18 89 774 84 703 3.32e-150 456
MS.gene52161.t1 AT3G03260 36.746 713 383 18 92 776 24 696 4.92e-142 434
MS.gene52161.t1 AT5G17320 35.088 741 416 21 73 777 7 718 1.53e-127 397
MS.gene52161.t1 AT1G34650 34.177 711 412 19 98 777 23 708 7.31e-116 367
MS.gene52161.t1 AT1G79840 40.359 446 219 7 88 530 98 499 1.53e-107 339
MS.gene52161.t1 AT3G03260 35.501 569 316 15 73 623 3 538 2.37e-104 331
MS.gene52161.t1 AT5G07260 27.321 560 325 16 157 712 10 491 1.32e-54 197
MS.gene52161.t1 AT5G07260 27.419 558 322 16 157 712 10 486 4.73e-54 196
MS.gene52161.t1 AT4G26920 28.829 222 145 9 528 746 231 442 4.22e-14 75.9
MS.gene52161.t1 AT1G30490 40.000 100 53 3 79 174 6 102 1.09e-12 72.4

Find 183 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTTGTGTTCTTCTATTT+TGG 0.092193 7.4:-29399295 None:intergenic
GGCTCTTGTTGAAACTTTAA+TGG 0.179447 7.4:+29400201 MS.gene52161:CDS
CAGTTGTAGAGCACCATTTC+TGG 0.218972 7.4:-29400769 None:intergenic
ATGCCTTTAGGTCAAGATTT+TGG 0.233318 7.4:+29399869 MS.gene52161:CDS
CTAAGCTTAGGCATGGGTTT+TGG 0.241414 7.4:+29403240 MS.gene52161:CDS
TCAAACCATTATTTGTTTGA+TGG 0.255996 7.4:-29398932 None:intergenic
GAGTTTGGTAACGACGTAAT+TGG 0.264874 7.4:-29399797 None:intergenic
GTGATATCTAGTGGAATAAA+TGG 0.274598 7.4:+29400744 MS.gene52161:CDS
CACAAACTAATGAGCCTCTT+TGG 0.274681 7.4:+29400035 MS.gene52161:CDS
GATAACATGGATGGTATTTC+TGG 0.280364 7.4:+29399002 MS.gene52161:CDS
AATTTCCATCAAACAAATAA+TGG 0.293878 7.4:+29398927 MS.gene52161:CDS
AGCTTCTCTGATGGTATCTA+TGG 0.310496 7.4:-29401043 None:intergenic
ACACTTGCCGGCAAGTTTCT+AGG 0.314978 7.4:+29399770 MS.gene52161:CDS
CCAATTTCTAAACTCGAGTT+TGG 0.322467 7.4:-29399812 None:intergenic
CCAAACTCGAGTTTAGAAAT+TGG 0.322627 7.4:+29399812 MS.gene52161:CDS
AAACAAGACAAGTCAAGTTT+TGG 0.322654 7.4:+29399270 MS.gene52161:CDS
GGAATGTGGGCGAGGACGTT+AGG 0.329094 7.4:+29403601 MS.gene52161:CDS
ACTTAGCAAAAGGCTTTGTT+TGG 0.341498 7.4:+29399247 MS.gene52161:CDS
TATGTTTCTTGAACTTGCTT+TGG 0.349553 7.4:+29399985 MS.gene52161:CDS
AACTGTAGGAGGCTTCCTTC+TGG 0.355417 7.4:+29401090 MS.gene52161:CDS
TTGCCTTCAACAATGCCTTT+AGG 0.357400 7.4:+29399857 MS.gene52161:CDS
GACCGAGTTTGTACACTTGC+CGG 0.358469 7.4:+29399758 MS.gene52161:CDS
TTCATTACTACATGCATTGC+GGG 0.358765 7.4:-29405741 None:intergenic
GGTGCACAAATGGAACAAAT+TGG 0.362490 7.4:+29403572 MS.gene52161:CDS
CACAAAAGTTGTTTGTCATT+CGG 0.369193 7.4:-29403530 None:intergenic
TGAACTTGCTTTGGCTGCAA+TGG 0.370269 7.4:+29399994 MS.gene52161:CDS
AAATCCATGTTCAACTCATC+TGG 0.372663 7.4:+29398734 MS.gene52161:CDS
TCGTTTATCAACTCCTACTT+TGG 0.381588 7.4:+29398709 MS.gene52161:CDS
ATTATTAGTACTCCTTGTAT+TGG 0.382879 7.4:+29400112 MS.gene52161:CDS
GAAATTTCACTAATAATTGC+TGG 0.383011 7.4:-29399683 None:intergenic
GCCTCAGAAACAAAACCATT+TGG 0.385246 7.4:-29400142 None:intergenic
TGGTGATATGAAGAGATTAA+TGG 0.385985 7.4:+29398899 MS.gene52161:CDS
GTCTATAGAGCTGGTGAACT+TGG 0.390142 7.4:-29403212 None:intergenic
GTGCATCGACGGTGCACAAA+TGG 0.392526 7.4:+29403562 MS.gene52161:CDS
TTTGTTCCTCTTGTAATTGA+AGG 0.396389 7.4:-29399911 None:intergenic
TATGCCAAATGGTTACTCTA+AGG 0.401101 7.4:+29401128 MS.gene52161:CDS
AGCAGCATGCAGAAGGGGTA+TGG 0.402136 7.4:+29401004 MS.gene52161:CDS
GAAGGCTATTCACAAGTATC+TGG 0.404248 7.4:-29405899 None:intergenic
TTGATTATATCAGACTCAAT+TGG 0.408893 7.4:+29399553 MS.gene52161:intron
GTTGCCATGGTCATGTCCTT+TGG 0.409163 7.4:-29403808 None:intergenic
AATTACAAGAGGAACAAATA+TGG 0.409950 7.4:+29399916 MS.gene52161:CDS
GGAATAAATGGAACCAGAAA+TGG 0.422357 7.4:+29400756 MS.gene52161:CDS
ATTACCTTAGAGTAACCATT+TGG 0.422663 7.4:-29401132 None:intergenic
CTCCCGTCGCTATGTCCATC+TGG 0.423743 7.4:-29405816 None:intergenic
TGGACGGCCTAGAAACTTGC+CGG 0.428194 7.4:-29399777 None:intergenic
GAACATGAAAGCTGCAGATC+TGG 0.433822 7.4:+29398975 MS.gene52161:CDS
AAGGAAGAGTGTGGATGATC+CGG 0.443006 7.4:+29403632 MS.gene52161:CDS
TGTATTGGTTTAAAACCAAA+TGG 0.443568 7.4:+29400127 MS.gene52161:CDS
CTCTTGCATTGGACCACCAT+TGG 0.446395 7.4:-29403772 None:intergenic
TGACCTAAAGGCATTGTTGA+AGG 0.455945 7.4:-29399860 None:intergenic
GGATGATCCGGGTGAGCCAC+CGG 0.456267 7.4:+29403644 MS.gene52161:CDS
CAGAAATGGTGCTCTACAAC+TGG 0.456843 7.4:+29400770 MS.gene52161:CDS
GGTTGTGAGATTGCACTAGA+AGG 0.460615 7.4:-29398686 None:intergenic
GAAACAAGCACAGACGCATC+GGG 0.460821 7.4:+29405687 MS.gene52161:CDS
GACATTCACATTGGCGTTGG+AGG 0.461547 7.4:-29403284 None:intergenic
AGAAAACTCACCTCACGAAC+AGG 0.469601 7.4:-29400970 None:intergenic
GAATGTGGGCGAGGACGTTA+GGG 0.470341 7.4:+29403602 MS.gene52161:CDS
ACAATCCCCGGTGGCTCACC+CGG 0.478000 7.4:-29403651 None:intergenic
TTATGTTGCGCTTCTTCCGT+CGG 0.480355 7.4:+29405779 MS.gene52161:CDS
AAAGACTTGAACTTAGCAAA+AGG 0.481146 7.4:+29399237 MS.gene52161:CDS
TTAGTGCAGCAACCTCAGTT+TGG 0.483693 7.4:+29403676 MS.gene52161:CDS
ACGAGAAGCTTAGAAGTGAA+TGG 0.489778 7.4:+29403739 MS.gene52161:CDS
TATGTTGCGCTTCTTCCGTC+GGG 0.492269 7.4:+29405780 MS.gene52161:CDS
CAAATTGGATGCAGGGAATG+TGG 0.492429 7.4:+29403587 MS.gene52161:CDS
TGGATTAGAAGTGTTGAAAG+TGG 0.496742 7.4:+29400055 MS.gene52161:CDS
GATGATCCGGGTGAGCCACC+GGG 0.500082 7.4:+29403645 MS.gene52161:CDS
AAATGGAACAAATTGGATGC+AGG 0.503062 7.4:+29403579 MS.gene52161:CDS
ATACCTGAGTGTTCTCTAGC+GGG 0.506667 7.4:-29403333 None:intergenic
CTAGACATTCACATTGGCGT+TGG 0.506942 7.4:-29403287 None:intergenic
CACGGTGGAATCAGTGGAAA+CGG 0.508426 7.4:+29405935 MS.gene52161:CDS
ATGCCAAAATCTTGACCTAA+AGG 0.510136 7.4:-29399872 None:intergenic
CCTCCTACAGTTAAGAAAAG+TGG 0.511269 7.4:-29401079 None:intergenic
CGTCCACAATTTGAACACAT+TGG 0.511856 7.4:-29399659 None:intergenic
TGCAGAAGGGGTATGGGCAG+TGG 0.512805 7.4:+29401011 MS.gene52161:CDS
ACTTCAAGTACTTTCTCCAT+TGG 0.513136 7.4:+29400945 MS.gene52161:CDS
CGAGAAGCTTAGAAGTGAAT+GGG 0.514863 7.4:+29403740 MS.gene52161:CDS
GGTGGAGCATGCAGAATATG+AGG 0.519622 7.4:+29403185 MS.gene52161:CDS
ACTAGATATCACTTCAGTTG+TGG 0.533585 7.4:-29400733 None:intergenic
GAGGCTTCCTTCTGGTTGTG+TGG 0.534040 7.4:+29401098 MS.gene52161:CDS
TTCTAAGCTTCTCGTTGCGA+AGG 0.534552 7.4:-29403731 None:intergenic
ACCCTTGCTAAGCTTAGGCA+TGG 0.536023 7.4:+29403233 MS.gene52161:CDS
CACCGTCGATGCACAAACTC+CGG 0.536497 7.4:-29403553 None:intergenic
CCGTGAGCTTTGCCGTCGGA+AGG 0.537799 7.4:-29405917 None:intergenic
CTCCTACAGTTAAGAAAAGT+GGG 0.539174 7.4:-29401078 None:intergenic
AGGAAGAGTGTGGATGATCC+GGG 0.542002 7.4:+29403633 MS.gene52161:CDS
TCTGAGGCTTCTAGAGAAAG+TGG 0.543217 7.4:+29400157 MS.gene52161:CDS
TCAAGTTCTTGAATTTGTTG+AGG 0.544285 7.4:-29399083 None:intergenic
AACATGGATTTAACCAAAGT+AGG 0.544723 7.4:-29398722 None:intergenic
AAATAGAAGAACACAAATGA+AGG 0.546022 7.4:+29399298 MS.gene52161:CDS
GCAGCATGCAGAAGGGGTAT+GGG 0.548640 7.4:+29401005 MS.gene52161:CDS
AATCCAATGTGTTCAAATTG+TGG 0.549032 7.4:+29399656 MS.gene52161:CDS
CAGATCTGGTAGTGATAACA+TGG 0.549386 7.4:+29398989 MS.gene52161:CDS
TGCCGGAGTTTGTGCATCGA+CGG 0.549484 7.4:+29403551 MS.gene52161:CDS
CCCTTGCTAAGCTTAGGCAT+GGG 0.551648 7.4:+29403234 MS.gene52161:CDS
TCCATTGGTTCCTGTTCGTG+AGG 0.554096 7.4:+29400960 MS.gene52161:CDS
GGTAATGACAAGGAAGAGTG+TGG 0.554273 7.4:+29403623 MS.gene52161:CDS
TAGCAATAAATGCGGGAGGA+AGG 0.555245 7.4:+29403484 MS.gene52161:intron
ATGTGAGCCATCTCTTGCAT+TGG 0.555705 7.4:-29403783 None:intergenic
TGCATACACTAGCTCGTAGG+AGG 0.558654 7.4:-29403839 None:intergenic
AGAAACAAGCACAGACGCAT+CGG 0.558699 7.4:+29405686 MS.gene52161:CDS
ACATTCACATTGGCGTTGGA+GGG 0.559350 7.4:-29403283 None:intergenic
TCAACACTTCTAATCCAAAG+AGG 0.561610 7.4:-29400049 None:intergenic
GGGTCGCAGCGGAGAGCGAG+TGG 0.562161 7.4:+29405855 MS.gene52161:CDS
AAATTGGATGCAGGGAATGT+GGG 0.562535 7.4:+29403588 MS.gene52161:CDS
AACATATGATGCAGGTGACA+TGG 0.564953 7.4:+29403163 MS.gene52161:intron
TTAGCAAGGGTCTATAGAGC+TGG 0.566123 7.4:-29403221 None:intergenic
TGTTGTTATCATAAACAGCT+TGG 0.566826 7.4:+29400180 MS.gene52161:CDS
ACCCATGCCTAAGCTTAGCA+AGG 0.568460 7.4:-29403235 None:intergenic
ACCTCACGAACAGGAACCAA+TGG 0.570995 7.4:-29400961 None:intergenic
ACCATCAGAGAAGCTTCAGC+TGG 0.571588 7.4:+29401051 MS.gene52161:CDS
GTAACAAATACTAATAGTAA+TGG 0.574242 7.4:+29399938 MS.gene52161:CDS
CTGTTGTATAACAGAATCGA+TGG 0.574417 7.4:+29400676 MS.gene52161:intron
GAGCTTCTTGCAATCACACA+AGG 0.574571 7.4:-29400711 None:intergenic
GTCGCTTGTTGTGTACGCGC+CGG 0.575675 7.4:+29405710 MS.gene52161:CDS
GGTTTCGCCGTGTTACCAGA+TGG 0.577544 7.4:+29405801 MS.gene52161:CDS
GGATGCAGGGAATGTGGGCG+AGG 0.578994 7.4:+29403593 MS.gene52161:CDS
AATGGAACAAATTGGATGCA+GGG 0.583192 7.4:+29403580 MS.gene52161:CDS
AATGGATGAGTTAGTGAAGA+TGG 0.583650 7.4:+29400012 MS.gene52161:CDS
TGCATTGCGGGAATATCTAC+CGG 0.586205 7.4:-29405729 None:intergenic
GGAGTGGTAATCACGATGAC+GGG 0.588681 7.4:+29405835 MS.gene52161:CDS
GAGTGATGATGACATTAAGA+TGG 0.589154 7.4:-29403310 None:intergenic
CCTTCCGACGGCAAAGCTCA+CGG 0.589815 7.4:+29405917 MS.gene52161:CDS
TACCAGATGGACATAGCGAC+GGG 0.591264 7.4:+29405814 MS.gene52161:CDS
AGAAACATAGACCTCTCCAT+TGG 0.591789 7.4:-29399971 None:intergenic
GCTGCACTAAGCACAATCCC+CGG 0.592750 7.4:-29403663 None:intergenic
AAACAAATAATGGTTTGAGA+AGG 0.592932 7.4:+29398937 MS.gene52161:CDS
ACAACTGAAGTGATATCTAG+TGG 0.593868 7.4:+29400735 MS.gene52161:CDS
TGGTGGTCCAATGCAAGAGA+TGG 0.598545 7.4:+29403776 MS.gene52161:CDS
TTGCAAGCAGCATGCAGAAG+GGG 0.600217 7.4:+29400999 MS.gene52161:CDS
ACCAGCTGAAGCTTCTCTGA+TGG 0.600752 7.4:-29401052 None:intergenic
GTGGTTCAAGATATGCCAAA+TGG 0.603358 7.4:+29401117 MS.gene52161:CDS
TTAATCATGAGGAATATACA+AGG 0.603991 7.4:+29400089 MS.gene52161:CDS
GATGGACATAGCGACGGGAG+TGG 0.607388 7.4:+29405819 MS.gene52161:CDS
GTTCCTCTTGTAATTGAAGG+TGG 0.608032 7.4:-29399908 None:intergenic
CTATGTGTAGCAATAAATGC+GGG 0.610261 7.4:+29403477 MS.gene52161:intron
TCACCACCTTCAATTACAAG+AGG 0.610841 7.4:+29399905 MS.gene52161:CDS
GGAGGGAGACACAGTTGCCA+TGG 0.612613 7.4:-29403821 None:intergenic
CCCATGCCTAAGCTTAGCAA+GGG 0.614032 7.4:-29403234 None:intergenic
GAATTTGTTGAGGAGTGTGT+CGG 0.614112 7.4:-29399073 None:intergenic
AAAGCTCACGGTGGAATCAG+TGG 0.620306 7.4:+29405929 MS.gene52161:CDS
TCCACCGTGAGCTTTGCCGT+CGG 0.623528 7.4:-29405921 None:intergenic
GTCACGAGAATTCACTACTT+AGG 0.625972 7.4:+29399579 MS.gene52161:CDS
TCTGGTAGTGATAACATGGA+TGG 0.630519 7.4:+29398993 MS.gene52161:CDS
GAATGGGACATACTCTCCAA+TGG 0.631124 7.4:+29403756 MS.gene52161:CDS
GGGAGTGGTAATCACGATGA+CGG 0.631663 7.4:+29405834 MS.gene52161:CDS
TGCCGGCAAGTGTACAAACT+CGG 0.632620 7.4:-29399760 None:intergenic
TTTGCAAGCAGCATGCAGAA+GGG 0.633056 7.4:+29400998 MS.gene52161:CDS
TAAACCAGATGAGTTGAACA+TGG 0.634149 7.4:-29398738 None:intergenic
TCTTGAACCACACAACCAGA+AGG 0.644378 7.4:-29401105 None:intergenic
CCTATGTGTAGCAATAAATG+CGG 0.644689 7.4:+29403476 MS.gene52161:intron
GTTCATTACTACATGCATTG+CGG 0.652040 7.4:-29405742 None:intergenic
TTGGTAACGACGTAATTGGA+CGG 0.652910 7.4:-29399793 None:intergenic
ATATGCATACACTAGCTCGT+AGG 0.653193 7.4:-29403842 None:intergenic
TATAGACCCTTGCTAAGCTT+AGG 0.654709 7.4:+29403228 MS.gene52161:CDS
ACATATGATGCAGGTGACAT+GGG 0.655326 7.4:+29403164 MS.gene52161:intron
GCATACACTAGCTCGTAGGA+GGG 0.655354 7.4:-29403838 None:intergenic
GAGATGGCTCACATTGCCAA+AGG 0.656074 7.4:+29403792 MS.gene52161:CDS
ATCACGATGACGGGTCGCAG+CGG 0.657275 7.4:+29405844 MS.gene52161:CDS
GGTAACACGGCGAAACCCGA+CGG 0.657528 7.4:-29405795 None:intergenic
AGATGGCTAGACATTCACAT+TGG 0.658957 7.4:-29403293 None:intergenic
GCAATGCATGTAGTAATGAA+CGG 0.659870 7.4:+29405744 MS.gene52161:CDS
TTACCAGATGGACATAGCGA+CGG 0.660884 7.4:+29405813 MS.gene52161:CDS
TATACCTGAGTGTTCTCTAG+CGG 0.665145 7.4:-29403334 None:intergenic
TGTCAATAAGAGAAGCAATG+AGG 0.665809 7.4:+29399633 MS.gene52161:CDS
GCTATGTCCATCTGGTAACA+CGG 0.669438 7.4:-29405808 None:intergenic
ACTTGTGAATAGCCTTCCGA+CGG 0.670143 7.4:+29405905 MS.gene52161:CDS
ATGATCCGGGTGAGCCACCG+GGG 0.670578 7.4:+29403646 MS.gene52161:CDS
TACCTGAGTGTTCTCTAGCG+GGG 0.673914 7.4:-29403332 None:intergenic
TGGGACATACTCTCCAATGG+TGG 0.679385 7.4:+29403759 MS.gene52161:CDS
TATGATGCAGGTGACATGGG+TGG 0.686232 7.4:+29403167 MS.gene52161:intron
CAAACAAATATAGAAGCACA+AGG 0.687269 7.4:+29398876 MS.gene52161:CDS
TGTGTAGCAATAAATGCGGG+AGG 0.696223 7.4:+29403480 MS.gene52161:intron
ATTGCCAAAGGACATGACCA+TGG 0.701545 7.4:+29403804 MS.gene52161:CDS
GCACTAAGCACAATCCCCGG+TGG 0.713315 7.4:-29403660 None:intergenic
AGCTTCTTGCAATCACACAA+GGG 0.721070 7.4:-29400710 None:intergenic
AAACAAGCACAGACGCATCG+GGG 0.726013 7.4:+29405688 MS.gene52161:CDS
AGGACGTTAGGGTAATGACA+AGG 0.727528 7.4:+29403613 MS.gene52161:CDS
TGAAGCGGGTAGCCAAACTG+AGG 0.731803 7.4:-29403688 None:intergenic
CTCCCCGCTAGAGAACACTC+AGG 0.744703 7.4:+29403330 MS.gene52161:CDS
TCCGACGGCAAAGCTCACGG+TGG 0.748267 7.4:+29405920 MS.gene52161:CDS
ACAAATATAGAAGCACAAGG+TGG 0.748500 7.4:+29398879 MS.gene52161:CDS
AAAGTAGGAGTTGATAAACG+AGG 0.750797 7.4:-29398707 None:intergenic
ATGCATGTAGTAATGAACGG+CGG 0.775511 7.4:+29405747 MS.gene52161:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.4 gene 29398683 29406019 29398683 ID=MS.gene52161
chr7.4 mRNA 29398683 29406019 29398683 ID=MS.gene52161.t1;Parent=MS.gene52161
chr7.4 exon 29398683 29398772 29398683 ID=MS.gene52161.t1.exon1;Parent=MS.gene52161.t1
chr7.4 CDS 29398683 29398772 29398683 ID=cds.MS.gene52161.t1;Parent=MS.gene52161.t1
chr7.4 exon 29398876 29399108 29398876 ID=MS.gene52161.t1.exon2;Parent=MS.gene52161.t1
chr7.4 CDS 29398876 29399108 29398876 ID=cds.MS.gene52161.t1;Parent=MS.gene52161.t1
chr7.4 exon 29399202 29399319 29399202 ID=MS.gene52161.t1.exon3;Parent=MS.gene52161.t1
chr7.4 CDS 29399202 29399319 29399202 ID=cds.MS.gene52161.t1;Parent=MS.gene52161.t1
chr7.4 exon 29399566 29400228 29399566 ID=MS.gene52161.t1.exon4;Parent=MS.gene52161.t1
chr7.4 CDS 29399566 29400228 29399566 ID=cds.MS.gene52161.t1;Parent=MS.gene52161.t1
chr7.4 exon 29400690 29400791 29400690 ID=MS.gene52161.t1.exon5;Parent=MS.gene52161.t1
chr7.4 CDS 29400690 29400791 29400690 ID=cds.MS.gene52161.t1;Parent=MS.gene52161.t1
chr7.4 exon 29400934 29401149 29400934 ID=MS.gene52161.t1.exon6;Parent=MS.gene52161.t1
chr7.4 CDS 29400934 29401149 29400934 ID=cds.MS.gene52161.t1;Parent=MS.gene52161.t1
chr7.4 exon 29403177 29403351 29403177 ID=MS.gene52161.t1.exon7;Parent=MS.gene52161.t1
chr7.4 CDS 29403177 29403351 29403177 ID=cds.MS.gene52161.t1;Parent=MS.gene52161.t1
chr7.4 exon 29403487 29403854 29403487 ID=MS.gene52161.t1.exon8;Parent=MS.gene52161.t1
chr7.4 CDS 29403487 29403854 29403487 ID=cds.MS.gene52161.t1;Parent=MS.gene52161.t1
chr7.4 exon 29405648 29406019 29405648 ID=MS.gene52161.t1.exon9;Parent=MS.gene52161.t1
chr7.4 CDS 29405648 29406019 29405648 ID=cds.MS.gene52161.t1;Parent=MS.gene52161.t1
Gene Sequence

>MS.gene52161

ATGCCTTCTAGTGCAATCTCACAACCTCGTTTATCAACTCCTACTTTGGTTAAATCCATGTTCAACTCATCTGGTTTATCTTTAGCACTTGTAAGAGATTATTTTCTTTTCATACTATAATATGATTTTATATTTTTACTTCAATCAATTTTTTATGTTCTTCTTGTTTGGGAAATATTGAATATTATTACAGCAAACAAATATAGAAGCACAAGGTGGTGATATGAAGAGATTAATGGCAGAAAATTTCCATCAAACAAATAATGGTTTGAGAAGGAATAGAGAAGAAGAAGAACATGAAAGCTGCAGATCTGGTAGTGATAACATGGATGGTATTTCTGGTGATGATATTGATGCTGCCGACAATCCGCCAAGAAAAAAACGTTATCACCGACACACTCCTCAACAAATTCAAGAACTTGAATCGTATGTATCATAATTTCCTTCAAAAAAAAGTTCATCTTTTTTATGAAGTTTATAAAAAATAATTGTTTGGTTTTTGTCTTTTATATTTTGTAGGATGTTTAAAGAGTGTCCTCACCCTGATGAAAAACAAAGACTTGAACTTAGCAAAAGGCTTTGTTTGGAAACAAGACAAGTCAAGTTTTGGTTCCAAAATAGAAGAACACAAATGAAGGTAACCAAAAAAACACTTTCCAAAAGTCTTTGTATTCACATGGAATAGTATCTTTTTCTTTTGGAGTTCAATATTTTCATACGGCTCCTAGAAGAAGAAAAAAAAAGTTGAAATAACAACATAAATTTAGAAACAAAAAATGGTAAAAACCGGTCTCTAATTTTATTTTTTTCGTCTCTAATTTTTGTCGTTATTTCAACTTTTTCTAATTTTGTCGCTAATTTTATTTTTTGTTGATTATATCAGACTCAATTGGAACGTCACGAGAATTCACTACTTAGGCAAGCAAATGATAAATTAAGAGCTGAGAACATGTCAATAAGAGAAGCAATGAGGAATCCAATGTGTTCAAATTGTGGACGACCAGCAATTATTAGTGAAATTTCACTTGAAGAACAACATCTAAGAATTGAGAATGCAAGATTAAAAGATGAATTAGACCGAGTTTGTACACTTGCCGGCAAGTTTCTAGGCCGTCCAATTACGTCGTTACCAAACTCGAGTTTAGAAATTGGTTTTAATGGTTTGAACAATACTTTGCCTTCAACAATGCCTTTAGGTCAAGATTTTGGCATGGTGTCAATGTCACCACCTTCAATTACAAGAGGAACAAATATGGTAACAAATACTAATAGTAATGGTTTTGATAGACCAATGGAGAGGTCTATGTTTCTTGAACTTGCTTTGGCTGCAATGGATGAGTTAGTGAAGATGGCACAAACTAATGAGCCTCTTTGGATTAGAAGTGTTGAAAGTGGAAAAGAAATTTTTAATCATGAGGAATATACAAGGATTATTAGTACTCCTTGTATTGGTTTAAAACCAAATGGTTTTGTTTCTGAGGCTTCTAGAGAAAGTGGTGTTGTTATCATAAACAGCTTGGCTCTTGTTGAAACTTTAATGGATTCAGTAAGCCTTTTTCAACTACTTCATTCAATTTTCTCCAACTTCTCTTCTTAGCCTTTCAATCATTCTTGGCTTAGGATGATTTATTTATTTTTTTCATTTTACTTGGATGAATATATTTAGTACTATACTAGAATTCATTAAGAAAAAATTGAAGAAATGAAAATTATTATTAATACCTGTAACACTAATGACTACTCCAATATTTACAATTATCTCATGTTTAATTTGAACTATGTCATGGAGGTTAGTAATCAAGCTATTTTTTACTACTAGTTTACTACTACAAAATATTTTAGAAACTAGAAAGCAATTTGTATGATGATAAAATTATCATAGACAAATTTTATATTTTGTCTTAATAAATTTATGTTGCAAATATAATTTTTTAGTGGTGATTTATTAATAATAATATATATTAAATTGTTGTACTTGAAACTGATTTTGTTTCTGTTGTATAACAGAATCGATGGTCAGAGATGTTCCCTTGTGTGATTGCAAGAAGCTCCACAACTGAAGTGATATCTAGTGGAATAAATGGAACCAGAAATGGTGCTCTACAACTGGTATATAAATTATAAATTATATAGTACATAAACAGAAATTTTTCCATTAATACTATAATGTTTTAGTTACCTTTTATTTTCATCACTTTAAAAATACTTCTATATATTTTTGTTTTAGCTTATGAAATTTATTTGTTTACAGATGCAAGCTGAACTTCAAGTACTTTCTCCATTGGTTCCTGTTCGTGAGGTGAGTTTTCTAAGGTTTTGCAAGCAGCATGCAGAAGGGGTATGGGCAGTGGTTGATGTGTCCATAGATACCATCAGAGAAGCTTCAGCTGGTGCACCCACTTTTCTTAACTGTAGGAGGCTTCCTTCTGGTTGTGTGGTTCAAGATATGCCAAATGGTTACTCTAAGGTAATTAAAAAAAAAAAAAAAACTCGTTAGCAAAACCCTTTTATAAAATACATGATGCGATGCCAATAGATGTATATAAAAAATTTACATTATTAGTTTATACAAATTAAAATTGAAATAGCGACGAAAATTTAAATAATGACGTAAAGTTAGTAACACAAAAATGCAAAATTAGCTTCTAACATTGGTTTACATGTTAGGGACATAATTTTTAGAAACCAATTTTGTATTTTTCATCTCTAATTTATGTTGTTTTTTCAATTTTTTCTAGTAGTGTGAATCATTTTATTTTATGTTTCAATTTACTGCATTTTTACCTTCCCAAATTCCAAAAAGTAATAAAGGATTCACAATCTGTCAAAAGTTGTATCCTAATAATTTTTGTAATATATGTAAAGGACACATATATAGTAAACCGTACTACTATCTCAACTGTTTTTTTTTCATCACATTTATTTATTTATTTATTTCAAGTTGGTGTACAAGTAATTGAAACATGGTAGCTGAATTGAGCAATATTTTAAAGTTGGGGAGGGGGAAAACACAAAGCTAAGGAATCATGGAGCTTTTGCTTTCCCAAGCAACTTAGAAAGCTCATGGTTCAAAGTAATATTTCAGCTTGTTTAGTGAGTATTCACTATAGAACCACTCTCTCAGTGATGGTTCCCATGTTATTAATTTTATAATGGGTCATGTTAACCAGTGTAAGGAAACTAAGAAAAGGAAGTTTTAAAATGAAAATAACACTTTTTATGTTTTATAAGAGTTAAAAACAAAAGTTTCAATGTAATACTTAATACATTTGATTTCTTAATCAATGATCCGGGAGCACCGGTTAACATTACTCTTTTATAATTAGTCTCTCAAATATGCAACCTGGGCTAGGATGCTTCCTCTCTTGTGTCTATCACAAAAATTATTTGAGTGATGAACACATCAATGTAGGTGAAATCTAGGTGAATCCATTAATTTAATCATGGAAGTCTCCATCACTTAAATTTTGGTAGCTGCATGAACACATTAATTACAAACCTTTTAAATAATTAATTTGTTGTTTTTTTAACTTTATCACATTCTATAAAAAAAATCATATGATGTTTCTATTCCAATCTCTCATGCAGGTTGTTCCCTATAAGAATATTATTTTAATTCATGAAATTGTGTGACCTGGTTTTGAACCCTATAAAAACATATAGGTCAGTTATATAATATATTCAATGTGGACCCTTTGAATCAAGATAATAGACTGAATATTTTGTCTTTTTGCTATAAGTGTTTGGGATGAGTTCACCCCACTGCAAAATATAGCAAATCTCGATGAACCTAATTTTATATTGCATGTAGCTTCTCTAAAATCCCGGATGATGACTTTATCTAGTAGTACCATTATTTTATGTATGTACTTGCTCGATCTCCATAAGATAGATACCTTGGTCTGTCGTTGAGTCAACTTGCCAGGCTTTCTCCAAGATTTTCTGTTCATACTTGTACATCATGGTCCATGAATCATGATCATAATCAATCAGCAGCTTCCTTCATTCCTGTTCAACATGTGTGAATGATGACAGCTTAGATACATCGTGAGAACGATAAAATTACCGCGAATACTCGTGATTTTGCCAAATTATAGTATATATGGGCTTTAATAAAATGACCATGTCACCGTAATCTTGTGAAACACATCGTGATTTCAAATATAACTACTAATTTCAGGAAAATTTAATAACTCGATTATTTAGAAGGATGCGTCGGACCATTATTAAAGCTTAGACTATGAAATTTGTAACGGACTTGGATCTTCTATAGCAGTTGTAGAATCATTAATTCTGATTGTAAGATTCAACAATAATAACTTACTTTTAGATCTAATAGCTAACAATTATGTTGTGGTGCAGCTGTTGTAGATAAAAAAAAATTATGTTTACAATAAACTTCGCAAAGTTGGGATTTCATGAATATGGTAGGAAAATGAGCATGGTAATGGTATAACTAATAACATGGTTGATTTACATGAACATATGATGCAGGTGACATGGGTGGAGCATGCAGAATATGAGGAAAGCCAAGTTCACCAGCTCTATAGACCCTTGCTAAGCTTAGGCATGGGTTTTGGCGCACAACGTTGGGTTGCAACCCTCCAACGCCAATGTGAATGTCTAGCCATCTTAATGTCATCATCACTCCCCGCTAGAGAACACTCAGGTATATATCCATCAATCCACACGTACCAATGTGGATCCTCAACTTACAGCTAGAGGATTCAAACACCATACACACACACATTTAACAAAATGTATTTGTCCACAATAACCCACGGTGTTATGTTCCTATGTGTAGCAATAAATGCGGGAGGAAGGCGTAGCATGCTAAAGCTAGCGCACCGAATGACAAACAACTTTTGTGCCGGAGTTTGTGCATCGACGGTGCACAAATGGAACAAATTGGATGCAGGGAATGTGGGCGAGGACGTTAGGGTAATGACAAGGAAGAGTGTGGATGATCCGGGTGAGCCACCGGGGATTGTGCTTAGTGCAGCAACCTCAGTTTGGCTACCCGCTTCACCACAAAAAGTCTTTGATTTCCTTCGCAACGAGAAGCTTAGAAGTGAATGGGACATACTCTCCAATGGTGGTCCAATGCAAGAGATGGCTCACATTGCCAAAGGACATGACCATGGCAACTGTGTCTCCCTCCTACGAGCTAGTGTATGCATATTCTTCCATCTTCAATATCATTTTCTTATAATATTTTGAGTTAAAATTAGATTTTTGAATTTTAGAATTCGTTTAGTGTTTTCTTATCTTTATTTTCCAAACTAATACCATAATTAAGTGTTTTTTATTACAATAATCAAGTGTTTTAAAAAATGGTTGACATGATTAGAAGATTAAAAAAAATAAATTTTATTTAGAATTTAATTATCATATATTACTAAAAAAATTGTATACAAAAATTTAGAGACAAAGACTTAAAGATGTATAAATATAATTTTTCATCTATAAATTTTATGTAAATATTTTATAAAACCATATTATTGCCGAACATTACCATAGTGAAATATGATTCAATTTGAGCTATTTATTTATGGGTCTTGCTAACTAGTGCCTTCAGGACAATGGTTAAGGAAACCAAAGTAGTATATTTACATTGAATTCAACAATGTTTTGACTTTTAAAAAGTTAAATTCTCCACTTTTCACCATTTTCCAATGCAATGTTTCTATTTTTATCATCTTAACCAATACCCCAAGGACACTATTTAGCATTTCCCTTTGTTTATATATGATCAAACTTTCTAAATTGATTGTAACAAAAAAAAAAAAACTTTCTAAATTGAAATTTAGAAATGAATGGTGTACGTTTTACTATTATTGATTTGATTGTTGTATGACGAGATAACGTTTTCAAGAGAAGAAGAAACGTGAATCTTGTTTTGTCGCGTAGTCATTGGATGAAAAGGTGAAATTGAGAAGGACAGGGTTGAAAATATTTGGAAACATCTATGAAGACAATTTGTAACAGAAAAGGGAAGCGTTTGTTTCAGCAAATTTTTCCAAAAAGTCAAAATCTGAAACTTTTGTCAACTTCCTACATTTTTCTAACTTTTGAGTTCTGACCTCATTCTTTTAACCTAAATTTTGATGTTTAGAAAAAGAAAACAAAAAATGTGGTACCAAGCAATGTTTCGCAAATCGAGTGTTAACACTCCGAGTTTACCGAGTTTACGTGTTTAGGTACCAAAATCGTGTTAACTATGAGGTAAACTCGGTTTCTGGTAAAATCGGAAGGTTTGACGAGTTTGACCGAGTTAACACTCGGTAAACTCGTATAAACTCGACCGAGTTTACTTACGAGTTTACATCTAAAAAAATAAAATAAAAATTGGCCATTTTTTCTGAAACTTGTTTTGTTGGATGGTTTTATGGATGGAATGCTTATGAAACTTATGATTTTTTGCATTAATTTTTATATTTTGATGCTAAGTTGAGAATAACGTTTTATTATGGTAGTTTGGTATAATTTGTCTTTGTAATTAAGTCATATTTTGCACTATTAACTTATTTACAATATTATATTAGCTTATATATGTCTTGCATATATTAAATTTTATATAATTTTAATTTTAGGTAAACTCGTACGAGTTCTCGAGTCGAGTTTACGAGTTGAGTTTACCTAGGCAAAACGAGTTGAGGTAAACTCTCGAGTTGTAAAACATTGGTACCAAGGTTTGACTTATTGACTACTCTCCAACCCTAAAACTTAACGAAAAAGACAACACATAAAATATAAATATTAATTTCGAAAAACATTCTTATATCATGAAATTTTGTTGGATATTTATTTTATCATTCAATTATTTATTTAATGTCATATCATCTTCTAATATTTAATTATTTCAAGCTAAATTAAAATTTGAATTGATTTTAGTTAAAAACCAACAAAACTCTAAAAATCAAATAATTTTTCATTTTCTTAACGTTGTTTTTTTCTCTTTGCAGGCTATTAATTCAAGTCAAAGTAGCATGTTGATTCTACAAGAAACAAGCACAGACGCATCGGGGTCGCTTGTTGTGTACGCGCCGGTAGATATTCCCGCAATGCATGTAGTAATGAACGGCGGCGATTCAGCTTATGTTGCGCTTCTTCCGTCGGGTTTCGCCGTGTTACCAGATGGACATAGCGACGGGAGTGGTAATCACGATGACGGGTCGCAGCGGAGAGCGAGTGGATCACTTTTGACGGTAGCATTCCAGATACTTGTGAATAGCCTTCCGACGGCAAAGCTCACGGTGGAATCAGTGGAAACGGTGAACAATCTCATTTCATGTACTATTCAAAAGATAAAAGTAGCACTTCAATGTGAGAGTTAA
Protein sequence

>MS.gene52161.t1

MPSSAISQPRLSTPTLVKSMFNSSGLSLALQTNIEAQGGDMKRLMAENFHQTNNGLRRNREEEEHESCRSGSDNMDGISGDDIDAADNPPRKKRYHRHTPQQIQELESMFKECPHPDEKQRLELSKRLCLETRQVKFWFQNRRTQMKTQLERHENSLLRQANDKLRAENMSIREAMRNPMCSNCGRPAIISEISLEEQHLRIENARLKDELDRVCTLAGKFLGRPITSLPNSSLEIGFNGLNNTLPSTMPLGQDFGMVSMSPPSITRGTNMVTNTNSNGFDRPMERSMFLELALAAMDELVKMAQTNEPLWIRSVESGKEIFNHEEYTRIISTPCIGLKPNGFVSEASRESGVVIINSLALVETLMDSNRWSEMFPCVIARSSTTEVISSGINGTRNGALQLMQAELQVLSPLVPVREVSFLRFCKQHAEGVWAVVDVSIDTIREASAGAPTFLNCRRLPSGCVVQDMPNGYSKVTWVEHAEYEESQVHQLYRPLLSLGMGFGAQRWVATLQRQCECLAILMSSSLPAREHSAINAGGRRSMLKLAHRMTNNFCAGVCASTVHKWNKLDAGNVGEDVRVMTRKSVDDPGEPPGIVLSAATSVWLPASPQKVFDFLRNEKLRSEWDILSNGGPMQEMAHIAKGHDHGNCVSLLRASAINSSQSSMLILQETSTDASGSLVVYAPVDIPAMHVVMNGGDSAYVALLPSGFAVLPDGHSDGSGNHDDGSQRRASGSLLTVAFQILVNSLPTAKLTVESVETVNNLISCTIQKIKVALQCES