AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52394

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52394.t1 MTR_2g436400 92.857 280 20 0 1 280 102 381 0.0 530
MS.gene52394.t1 MTR_2g437060 79.570 279 57 0 2 280 111 389 7.65e-161 452
MS.gene52394.t1 MTR_2g437080 70.357 280 83 0 1 280 95 374 3.12e-140 400
MS.gene52394.t1 MTR_2g437030 69.286 280 86 0 1 280 181 460 1.41e-133 386
MS.gene52394.t1 MTR_2g436440 67.143 280 92 0 1 280 101 380 1.60e-130 375
MS.gene52394.t1 MTR_2g437020 65.480 281 94 2 2 280 95 374 6.05e-127 366
MS.gene52394.t1 MTR_2g437040 63.214 280 103 0 1 280 95 374 2.67e-123 357
MS.gene52394.t1 MTR_4g119550 53.025 281 131 1 1 280 104 384 6.08e-105 310
MS.gene52394.t1 MTR_2g437170 52.281 285 131 3 1 280 110 394 1.75e-98 295
MS.gene52394.t1 MTR_2g437960 51.237 283 135 2 1 280 108 390 5.54e-98 293
MS.gene52394.t1 MTR_2g437960 51.237 283 135 2 1 280 108 390 1.31e-97 293
MS.gene52394.t1 MTR_2g438010 50.877 285 133 3 1 280 125 407 7.45e-97 291
MS.gene52394.t1 MTR_2g437150 52.143 280 129 3 1 275 110 389 1.75e-96 290
MS.gene52394.t1 MTR_2g437180 50.896 279 132 2 1 276 104 380 2.22e-95 286
MS.gene52394.t1 MTR_2g437160 51.064 282 136 1 1 280 118 399 5.41e-95 286
MS.gene52394.t1 MTR_2g437100 53.025 281 127 3 1 276 115 395 1.18e-94 285
MS.gene52394.t1 MTR_2g437120 52.688 279 128 2 1 276 106 383 1.50e-94 285
MS.gene52394.t1 MTR_2g437130 49.643 280 141 0 1 280 96 375 4.79e-93 280
MS.gene52394.t1 MTR_2g437200 46.178 314 131 2 1 276 85 398 1.68e-92 279
MS.gene52394.t1 MTR_2g437990 48.582 282 143 1 1 280 105 386 4.84e-92 279
MS.gene52394.t1 MTR_2g076320 48.780 287 134 2 2 280 106 387 1.56e-90 274
MS.gene52394.t1 MTR_2g437260 46.570 277 146 1 1 275 89 365 7.98e-89 270
MS.gene52394.t1 MTR_2g437240 44.025 318 140 2 1 280 85 402 1.10e-88 270
MS.gene52394.t1 MTR_2g438020 45.541 314 133 2 1 276 124 437 1.35e-88 272
MS.gene52394.t1 MTR_2g436460 46.570 277 147 1 1 276 100 376 3.28e-85 260
MS.gene52394.t1 MTR_2g019840 48.592 284 138 4 1 280 96 375 2.59e-84 258
MS.gene52394.t1 MTR_2g437940 47.857 280 121 3 1 280 104 358 5.88e-80 246
MS.gene52394.t1 MTR_2g019810 46.071 280 147 3 1 278 84 361 1.80e-79 244
MS.gene52394.t1 MTR_2g437090 69.753 162 49 0 119 280 1 162 2.60e-78 235
MS.gene52394.t1 MTR_4g120380 42.701 274 157 0 1 274 97 370 1.44e-72 227
MS.gene52394.t1 MTR_4g119580 41.971 274 159 0 1 274 97 370 2.02e-71 224
MS.gene52394.t1 MTR_4g120380 42.913 254 145 0 1 254 97 350 8.44e-67 214
MS.gene52394.t1 MTR_2g437160 54.082 196 87 1 88 280 113 308 3.62e-66 209
MS.gene52394.t1 MTR_4g119570 36.842 247 123 2 26 272 14 227 3.11e-47 157
MS.gene52394.t1 MTR_3g450500 32.509 283 181 4 2 276 92 372 7.95e-45 155
MS.gene52394.t1 MTR_2g436370 77.895 95 21 0 1 95 101 195 1.22e-41 142
MS.gene52394.t1 MTR_4g007490 30.070 286 189 4 1 278 90 372 4.03e-36 132
MS.gene52394.t1 MTR_8g080990 29.259 270 187 3 4 270 100 368 9.01e-31 118
MS.gene52394.t1 MTR_2g436380 55.752 113 43 3 119 231 1 106 1.48e-30 116
MS.gene52394.t1 MTR_8g081000 26.259 278 200 3 1 274 109 385 9.21e-28 110
MS.gene52394.t1 MTR_8g081000 26.259 278 200 3 1 274 109 385 9.78e-28 110
MS.gene52394.t1 MTR_8g081000 26.259 278 200 3 1 274 109 385 1.34e-27 110
MS.gene52394.t1 MTR_3g085240 60.377 53 21 0 178 230 176 228 1.14e-15 75.1
MS.gene52394.t1 MTR_4g119615 60.000 50 20 0 218 267 26 75 3.56e-14 67.4
MS.gene52394.t1 MTR_2g019830 52.857 70 32 1 207 276 131 199 1.55e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52394.t1 AT5G07900 35.842 279 173 3 1 273 121 399 9.74e-55 182
MS.gene52394.t1 AT1G21150 30.282 284 190 4 1 277 72 354 8.57e-37 134
MS.gene52394.t1 AT1G21150 30.282 284 190 4 1 277 108 390 2.24e-36 134
MS.gene52394.t1 AT1G21150 30.282 284 190 4 1 277 172 454 4.64e-36 134
MS.gene52394.t1 AT1G61980 26.861 309 175 6 1 265 104 405 1.86e-26 107
MS.gene52394.t1 AT1G61980 26.861 309 175 6 1 265 104 405 1.86e-26 107
MS.gene52394.t1 AT1G61980 26.861 309 175 6 1 265 104 405 1.86e-26 107
MS.gene52394.t1 AT5G64950 30.175 285 179 6 6 274 96 376 1.14e-24 102
MS.gene52394.t1 AT1G62010 25.442 283 172 5 20 265 109 389 5.24e-24 100
MS.gene52394.t1 AT3G46950 24.928 349 186 8 1 277 104 448 4.30e-23 98.6
MS.gene52394.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.45e-22 95.5
MS.gene52394.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.45e-22 95.5
MS.gene52394.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.45e-22 95.5
MS.gene52394.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.45e-22 95.5
MS.gene52394.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.45e-22 95.5
MS.gene52394.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.45e-22 95.5
MS.gene52394.t1 AT1G61970 25.753 299 179 6 1 259 104 399 3.45e-22 95.5
MS.gene52394.t1 AT1G62120 24.510 306 188 6 1 265 108 411 2.10e-21 93.6
MS.gene52394.t1 AT1G61990 25.321 312 187 5 1 273 104 408 6.61e-21 92.0
MS.gene52394.t1 AT1G61990 25.321 312 187 5 1 273 104 408 6.61e-21 92.0
MS.gene52394.t1 AT1G62085 25.217 345 172 10 1 265 107 445 9.10e-20 89.0
MS.gene52394.t1 AT1G62085 25.217 345 172 10 1 265 107 445 9.10e-20 89.0
MS.gene52394.t1 AT1G61960 23.782 349 189 8 1 273 104 451 3.31e-17 81.6
MS.gene52394.t1 AT1G62110 27.835 194 133 3 1 189 104 295 5.91e-15 74.7
MS.gene52394.t1 AT5G23930 22.985 335 182 8 1 264 107 436 6.25e-15 74.7

Find 48 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATCTGACAAGGAAATTT+TGG 0.119034 2.3:-54834578 MS.gene52394:CDS
TAAATTTAGTGATGAACTTT+TGG 0.199642 2.3:-54834207 MS.gene52394:CDS
GTGAAGGAATTAAAGGATTT+GGG 0.230293 2.3:-54834403 MS.gene52394:CDS
AGACCAACCCCATTTCTTAA+AGG 0.251996 2.3:+54834294 None:intergenic
GGTATTGATTACTGTTGTTT+TGG 0.274832 2.3:+54834339 None:intergenic
AAACTTAATGACAAAATATA+AGG 0.279924 2.3:+54834142 None:intergenic
GGTGAAGGAATTAAAGGATT+TGG 0.282534 2.3:-54834404 MS.gene52394:CDS
ATGGATGTTAACATACAATA+AGG 0.292032 2.3:+54834238 None:intergenic
AGGTTGGGACTATTTGATTC+TGG 0.292547 2.3:+54834623 None:intergenic
TGACAAAATATAAGGTTGTT+TGG 0.318444 2.3:+54834150 None:intergenic
AAACTTTGGCAACACCGTTT+TGG 0.327649 2.3:+54834729 None:intergenic
AAGTTGATACCTTTAAGAAA+TGG 0.365182 2.3:-54834303 MS.gene52394:CDS
AGTTGATACCTTTAAGAAAT+GGG 0.365271 2.3:-54834302 MS.gene52394:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2.3:-54834188 MS.gene52394:CDS
CAAACATTAAAACTTTGCTT+AGG 0.367331 2.3:-54834468 MS.gene52394:CDS
CAAGTCGGTGAAGGAATTAA+AGG 0.378802 2.3:-54834410 MS.gene52394:CDS
AACTTATCAGTCACTACAAA+TGG 0.416436 2.3:+54834034 None:intergenic
GTCAGAGGTAGAAGCACCTT+TGG 0.417863 2.3:+54834693 None:intergenic
AATAGTCCCAACCTATGAAT+TGG 0.421560 2.3:-54834614 MS.gene52394:CDS
TATCAACTTTCTCTTTCCAT+AGG 0.439630 2.3:+54834317 None:intergenic
GGTTTGCAGGAAAGCAGCTC+TGG 0.441213 2.3:+54834754 None:intergenic
AAACATTAAAACTTTGCTTA+GGG 0.450947 2.3:-54834467 MS.gene52394:CDS
ATCACAATGCTGATTGAGAA+TGG 0.457142 2.3:-54834502 MS.gene52394:CDS
GGAAAGCAGCTCTGGCATCT+TGG 0.471973 2.3:+54834762 None:intergenic
GAATCTGTACACCAATTCAT+AGG 0.478001 2.3:+54834603 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.480856 2.3:-54834189 MS.gene52394:CDS
CTGTACACCAATTCATAGGT+TGG 0.484595 2.3:+54834607 None:intergenic
AGGCTTGCATACAAAACTCT+AGG 0.506576 2.3:+54834646 None:intergenic
TCAGTTGGGTTGGGATGCTA+TGG 0.506950 2.3:-54834179 MS.gene52394:CDS
TGTACACCAATTCATAGGTT+GGG 0.507173 2.3:+54834608 None:intergenic
TCACAATGCTGATTGAGAAT+GGG 0.508211 2.3:-54834501 MS.gene52394:CDS
AATATAAGGTTGTTTGGCAA+TGG 0.508469 2.3:+54834156 None:intergenic
CAACAGTAATCAATACCCTA+TGG 0.513109 2.3:-54834333 MS.gene52394:CDS
ATCAACTTTCTCTTTCCATA+GGG 0.529759 2.3:+54834318 None:intergenic
GTGCAATTTCTTCTCAACAA+AGG 0.536369 2.3:-54834088 MS.gene52394:CDS
TTCCTGCAAACCCTCCAAAA+CGG 0.545667 2.3:-54834743 MS.gene52394:CDS
GTTGATACCTTTAAGAAATG+GGG 0.553181 2.3:-54834301 MS.gene52394:CDS
TTGGGACTATTTGATTCTGG+AGG 0.560239 2.3:+54834626 None:intergenic
AATTGCACAACAGCGGCCCT+CGG 0.562081 2.3:+54834103 None:intergenic
CAGATTCTTGCAATCTGACA+AGG 0.575407 2.3:-54834587 MS.gene52394:CDS
GGCTTGCATACAAAACTCTA+GGG 0.581368 2.3:+54834647 None:intergenic
ATACCTTTAAGAAATGGGGT+TGG 0.587688 2.3:-54834297 MS.gene52394:CDS
TGAAGGAATTAAAGGATTTG+GGG 0.597102 2.3:-54834402 MS.gene52394:CDS
TGACATGCTCAAGTCGGTGA+AGG 0.598383 2.3:-54834419 MS.gene52394:CDS
GACAATGTTAACAATGTCAG+AGG 0.618058 2.3:+54834678 None:intergenic
CACAATGCTGATTGAGAATG+GGG 0.632348 2.3:-54834500 MS.gene52394:CDS
GAGAAGAAATTGCACAACAG+CGG 0.635644 2.3:+54834096 None:intergenic
GAGACGTGACATGCTCAAGT+CGG 0.666473 2.3:-54834425 MS.gene52394:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTAATGACAAAATATA+AGG + chr2.3:54834579-54834598 None:intergenic 15.0%
!!! CACTAAATTTATTTTTTCAA+TGG + chr2.3:54834502-54834521 None:intergenic 15.0%
!!! TTATATTTTGTCATTAAGTT+TGG - chr2.3:54834578-54834597 MS.gene52394:CDS 15.0%
!! AAACATTAAAACTTTGCTTA+GGG - chr2.3:54834251-54834270 MS.gene52394:CDS 20.0%
!!! AAATTTAGTGATGAACTTTT+GGG - chr2.3:54834512-54834531 MS.gene52394:CDS 20.0%
!!! TAAATTTAGTGATGAACTTT+TGG - chr2.3:54834511-54834530 MS.gene52394:CDS 20.0%
!!! TTATCTTTTAAAGTTGTATG+AGG - chr2.3:54834749-54834768 MS.gene52394:CDS 20.0%
! ATGGATGTTAACATACAATA+AGG + chr2.3:54834483-54834502 None:intergenic 25.0%
! CAAACATTAAAACTTTGCTT+AGG - chr2.3:54834250-54834269 MS.gene52394:CDS 25.0%
! GAGAGAAAAAATTGAAACTT+TGG + chr2.3:54834006-54834025 None:intergenic 25.0%
! TGACAAAATATAAGGTTGTT+TGG + chr2.3:54834571-54834590 None:intergenic 25.0%
! TGTCATTAAGTTTGGAAAAA+AGG - chr2.3:54834586-54834605 MS.gene52394:CDS 25.0%
!! AATCCTTCAAAAACAACTTT+TGG - chr2.3:54834342-54834361 MS.gene52394:CDS 25.0%
!! AGTTGATACCTTTAAGAAAT+GGG - chr2.3:54834416-54834435 MS.gene52394:CDS 25.0%
!!! AAGTTGATACCTTTAAGAAA+TGG - chr2.3:54834415-54834434 MS.gene52394:CDS 25.0%
!!! TATAAAACGTTTTGAGAATG+AGG - chr2.3:54834722-54834741 MS.gene52394:CDS 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG - chr2.3:54834009-54834028 MS.gene52394:CDS 25.0%
AACTTATCAGTCACTACAAA+TGG + chr2.3:54834687-54834706 None:intergenic 30.0%
AATATAAGGTTGTTTGGCAA+TGG + chr2.3:54834565-54834584 None:intergenic 30.0%
ATCAACTTTCTCTTTCCATA+GGG + chr2.3:54834403-54834422 None:intergenic 30.0%
GTGAAGGAATTAAAGGATTT+GGG - chr2.3:54834315-54834334 MS.gene52394:CDS 30.0%
TATCAACTTTCTCTTTCCAT+AGG + chr2.3:54834404-54834423 None:intergenic 30.0%
TGAAGGAATTAAAGGATTTG+GGG - chr2.3:54834316-54834335 MS.gene52394:CDS 30.0%
! GTTGATACCTTTAAGAAATG+GGG - chr2.3:54834417-54834436 MS.gene52394:CDS 30.0%
!!! GGTATTGATTACTGTTGTTT+TGG + chr2.3:54834382-54834401 None:intergenic 30.0%
AATAGTCCCAACCTATGAAT+TGG - chr2.3:54834104-54834123 MS.gene52394:CDS 35.0%
CAACAGTAATCAATACCCTA+TGG - chr2.3:54834385-54834404 MS.gene52394:CDS 35.0%
GAATCTGTACACCAATTCAT+AGG + chr2.3:54834118-54834137 None:intergenic 35.0%
GACAATGTTAACAATGTCAG+AGG + chr2.3:54834043-54834062 None:intergenic 35.0%
GGTGAAGGAATTAAAGGATT+TGG - chr2.3:54834314-54834333 MS.gene52394:CDS 35.0%
GTGCAATTTCTTCTCAACAA+AGG - chr2.3:54834630-54834649 MS.gene52394:CDS 35.0%
TATGAGGAAAAACGCAATCT+AGG - chr2.3:54834765-54834784 MS.gene52394:CDS 35.0%
TGTACACCAATTCATAGGTT+GGG + chr2.3:54834113-54834132 None:intergenic 35.0%
! ATACCTTTAAGAAATGGGGT+TGG - chr2.3:54834421-54834440 MS.gene52394:CDS 35.0%
! ATCACAATGCTGATTGAGAA+TGG - chr2.3:54834216-54834235 MS.gene52394:CDS 35.0%
! GCAATCTGACAAGGAAATTT+TGG - chr2.3:54834140-54834159 MS.gene52394:CDS 35.0%
! TCACAATGCTGATTGAGAAT+GGG - chr2.3:54834217-54834236 MS.gene52394:CDS 35.0%
!! AACTTTTGGGTCAATCAGTT+GGG - chr2.3:54834525-54834544 MS.gene52394:CDS 35.0%
!!! GCACCAAAAGTTGTTTTTGA+AGG + chr2.3:54834348-54834367 None:intergenic 35.0%
AGACCAACCCCATTTCTTAA+AGG + chr2.3:54834427-54834446 None:intergenic 40.0%
AGGCTTGCATACAAAACTCT+AGG + chr2.3:54834075-54834094 None:intergenic 40.0%
AGGTTGGGACTATTTGATTC+TGG + chr2.3:54834098-54834117 None:intergenic 40.0%
CAAGTCGGTGAAGGAATTAA+AGG - chr2.3:54834308-54834327 MS.gene52394:CDS 40.0%
CAGATTCTTGCAATCTGACA+AGG - chr2.3:54834131-54834150 MS.gene52394:CDS 40.0%
CTGTACACCAATTCATAGGT+TGG + chr2.3:54834114-54834133 None:intergenic 40.0%
GAGAAGAAATTGCACAACAG+CGG + chr2.3:54834625-54834644 None:intergenic 40.0%
GGAAAAAAGGATCATTCCGA+GGG - chr2.3:54834599-54834618 MS.gene52394:CDS 40.0%
GGCTTGCATACAAAACTCTA+GGG + chr2.3:54834074-54834093 None:intergenic 40.0%
TGGAAAAAAGGATCATTCCG+AGG - chr2.3:54834598-54834617 MS.gene52394:CDS 40.0%
TTGGGACTATTTGATTCTGG+AGG + chr2.3:54834095-54834114 None:intergenic 40.0%
! AAACTTTGGCAACACCGTTT+TGG + chr2.3:54833992-54834011 None:intergenic 40.0%
! CACAATGCTGATTGAGAATG+GGG - chr2.3:54834218-54834237 MS.gene52394:CDS 40.0%
!! GAACTTTTGGGTCAATCAGT+TGG - chr2.3:54834524-54834543 MS.gene52394:CDS 40.0%
TTCCTGCAAACCCTCCAAAA+CGG - chr2.3:54833975-54833994 MS.gene52394:CDS 45.0%
TTGGGTCAATCAGTTGGGTT+GGG - chr2.3:54834530-54834549 MS.gene52394:CDS 45.0%
TTTGGGTCAATCAGTTGGGT+TGG - chr2.3:54834529-54834548 MS.gene52394:CDS 45.0%
!! TTTGGCAACACCGTTTTGGA+GGG + chr2.3:54833988-54834007 None:intergenic 45.0%
GAGACGTGACATGCTCAAGT+CGG - chr2.3:54834293-54834312 MS.gene52394:CDS 50.0%
TGACATGCTCAAGTCGGTGA+AGG - chr2.3:54834299-54834318 MS.gene52394:CDS 50.0%
! CTTTGGCAACACCGTTTTGG+AGG + chr2.3:54833989-54834008 None:intergenic 50.0%
!! GTCAGAGGTAGAAGCACCTT+TGG + chr2.3:54834028-54834047 None:intergenic 50.0%
!! TCAGTTGGGTTGGGATGCTA+TGG - chr2.3:54834539-54834558 MS.gene52394:CDS 50.0%
AATTGCACAACAGCGGCCCT+CGG + chr2.3:54834618-54834637 None:intergenic 55.0%
GGTTTGCAGGAAAGCAGCTC+TGG + chr2.3:54833967-54833986 None:intergenic 55.0%
! GGAAAGCAGCTCTGGCATCT+TGG + chr2.3:54833959-54833978 None:intergenic 55.0%
!!! CACCGTTTTGGAGGGTTTGC+AGG + chr2.3:54833980-54833999 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 54833949 54834791 54833949 ID=MS.gene52394
chr2.3 mRNA 54833949 54834791 54833949 ID=MS.gene52394.t1;Parent=MS.gene52394
chr2.3 exon 54833949 54834791 54833949 ID=MS.gene52394.t1.exon1;Parent=MS.gene52394.t1
chr2.3 CDS 54833949 54834791 54833949 ID=cds.MS.gene52394.t1;Parent=MS.gene52394.t1
Gene Sequence

>MS.gene52394

ATGATTGCCAAGATGCCAGAGCTGCTTTCCTGCAAACCCTCCAAAACGGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTACCTCTGACATTGTTAACATTGTCTCTACAAACCCTAGAGTTTTGTATGCAAGCCTCCAGAATCAAATAGTCCCAACCTATGAATTGGTGTACAGATTCTTGCAATCTGACAAGGAAATTTTGGCAAAATTGAGTTACAATGCAAATTTACTTTGTGATCGTACTGTGTATAATAACATCACAATGCTGATTGAGAATGGGGTGTCTGACTCAAACATTAAAACTTTGCTTAGGGAACAGACTCGAATATTCAAGAGACGTGACATGCTCAAGTCGGTGAAGGAATTAAAGGATTTGGGGTTTAATCCTTCAAAAACAACTTTTGGTGCAGCATTGCATGCCAAAACAACAGTAATCAATACCCTATGGAAAGAGAAAGTTGATACCTTTAAGAAATGGGGTTGGTCTGAAGAAGATTCTCTTGAAGCATTTAGAAAGAAACCTTATTGTATGTTAACATCCATTGAAAAAATAAATTTAGTGATGAACTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCATTGCCAAACAACCTTATATTTTGTCATTAAGTTTGGAAAAAAGGATCATTCCGAGGGCCGCTGTTGTGCAATTTCTTCTCAACAAAGGTTTGACAGATAAGAATGCAAGCTTAACTTATCCATTTGTAGTGACTGATAAGTTGTTTCTTAATATGTTTATAAAACGTTTTGAGAATGAGGCTTCTTATCTTTTAAAGTTGTATGAGGAAAAACGCAATCTAGGATAG
Protein sequence

>MS.gene52394.t1

MIAKMPELLSCKPSKTVLPKFQFFLSKGASTSDIVNIVSTNPRVLYASLQNQIVPTYELVYRFLQSDKEILAKLSYNANLLCDRTVYNNITMLIENGVSDSNIKTLLREQTRIFKRRDMLKSVKELKDLGFNPSKTTFGAALHAKTTVINTLWKEKVDTFKKWGWSEEDSLEAFRKKPYCMLTSIEKINLVMNFWVNQLGWDAMAIAKQPYILSLSLEKRIIPRAAVVQFLLNKGLTDKNASLTYPFVVTDKLFLNMFIKRFENEASYLLKLYEEKRNLG