AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52435

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52435.t1 MTR_2g437240 84.652 417 64 0 1 417 1 417 0.0 703
MS.gene52435.t1 MTR_2g437200 75.062 401 94 2 1 398 1 398 0.0 585
MS.gene52435.t1 MTR_2g438020 72.987 385 103 1 31 415 70 453 0.0 555
MS.gene52435.t1 MTR_2g437960 66.062 386 94 2 34 417 55 405 1.33e-176 499
MS.gene52435.t1 MTR_2g437170 65.610 410 94 5 19 416 36 410 1.65e-175 496
MS.gene52435.t1 MTR_2g437960 65.885 384 94 2 34 415 55 403 2.06e-175 497
MS.gene52435.t1 MTR_2g437150 64.428 402 98 4 19 410 36 402 2.16e-170 483
MS.gene52435.t1 MTR_2g437120 63.728 397 104 4 18 410 35 395 1.70e-168 479
MS.gene52435.t1 MTR_2g437180 59.674 429 117 6 1 410 1 392 1.28e-163 466
MS.gene52435.t1 MTR_2g437100 60.993 423 105 6 3 400 10 397 1.59e-161 461
MS.gene52435.t1 MTR_2g437990 62.564 390 108 3 31 418 49 402 5.67e-161 460
MS.gene52435.t1 MTR_2g438010 60.000 390 119 2 31 418 69 423 3.87e-159 455
MS.gene52435.t1 MTR_2g076320 61.795 390 112 2 31 418 49 403 5.35e-159 454
MS.gene52435.t1 MTR_2g437160 58.741 429 120 8 3 410 15 407 1.69e-155 446
MS.gene52435.t1 MTR_2g437940 55.300 434 113 6 3 418 4 374 4.89e-145 417
MS.gene52435.t1 MTR_2g437130 52.488 402 150 4 15 414 25 387 2.95e-131 383
MS.gene52435.t1 MTR_2g437260 48.662 411 167 6 1 405 1 373 9.11e-123 362
MS.gene52435.t1 MTR_2g437080 46.970 396 168 5 16 408 24 380 3.10e-116 344
MS.gene52435.t1 MTR_2g437060 46.734 398 166 5 17 406 34 393 6.69e-113 337
MS.gene52435.t1 MTR_2g436440 46.565 393 169 4 21 410 34 388 8.51e-113 336
MS.gene52435.t1 MTR_4g119550 47.480 377 159 3 31 406 50 388 3.49e-112 335
MS.gene52435.t1 MTR_2g437020 46.301 419 171 9 1 406 1 378 1.68e-110 330
MS.gene52435.t1 MTR_2g437030 42.891 422 190 5 2 410 85 468 4.73e-108 327
MS.gene52435.t1 MTR_2g437040 45.995 387 169 3 27 411 35 383 5.12e-108 323
MS.gene52435.t1 MTR_2g436400 42.857 378 176 3 27 402 42 381 1.11e-99 302
MS.gene52435.t1 MTR_2g436460 46.505 372 160 5 28 398 43 376 1.28e-95 291
MS.gene52435.t1 MTR_4g119580 41.867 375 176 3 25 395 33 369 4.34e-85 265
MS.gene52435.t1 MTR_2g437160 71.038 183 53 0 228 410 134 316 3.71e-84 260
MS.gene52435.t1 MTR_2g437160 33.750 240 88 8 3 174 15 251 1.82e-20 92.0
MS.gene52435.t1 MTR_4g120380 41.209 364 176 2 32 395 44 369 9.69e-82 256
MS.gene52435.t1 MTR_2g019810 40.750 400 184 8 7 398 5 359 3.26e-78 246
MS.gene52435.t1 MTR_2g019840 40.374 374 181 6 32 403 43 376 2.66e-77 245
MS.gene52435.t1 MTR_4g120380 41.449 345 164 2 32 376 44 350 3.49e-76 243
MS.gene52435.t1 MTR_3g085240 62.694 193 25 3 1 188 21 171 3.62e-65 209
MS.gene52435.t1 MTR_3g085240 60.870 92 19 1 298 389 174 248 2.71e-25 103
MS.gene52435.t1 MTR_2g437090 54.878 164 74 0 241 404 1 164 6.71e-57 185
MS.gene52435.t1 MTR_4g007490 28.065 367 225 3 32 395 37 367 2.97e-43 155
MS.gene52435.t1 MTR_3g450500 27.179 390 234 5 20 395 16 369 9.21e-43 154
MS.gene52435.t1 MTR_4g119570 36.437 247 121 2 148 394 17 227 2.69e-41 146
MS.gene52435.t1 MTR_2g436370 35.294 221 85 6 8 224 24 190 9.06e-31 117
MS.gene52435.t1 MTR_8g081000 25.816 337 211 3 61 395 85 384 1.77e-27 113
MS.gene52435.t1 MTR_8g081000 25.816 337 211 3 61 395 85 384 1.92e-27 113
MS.gene52435.t1 MTR_8g081000 25.816 337 211 3 61 395 85 384 2.59e-27 112
MS.gene52435.t1 MTR_8g080990 27.410 332 202 3 63 392 74 368 4.11e-27 111
MS.gene52435.t1 MTR_4g049400 42.236 161 69 5 1 137 2 162 3.21e-24 100
MS.gene52435.t1 MTR_2g436380 58.333 60 25 0 241 300 1 60 6.45e-16 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52435.t1 AT5G07900 30.894 369 211 4 33 395 69 399 3.27e-55 188
MS.gene52435.t1 AT1G21150 29.700 367 216 4 32 393 55 384 1.91e-40 148
MS.gene52435.t1 AT1G21150 29.700 367 216 4 32 393 119 448 7.17e-40 148
MS.gene52435.t1 AT1G21150 29.076 368 219 4 31 393 18 348 6.90e-39 143
MS.gene52435.t1 AT1G61980 28.093 388 204 7 31 381 50 399 4.60e-36 137
MS.gene52435.t1 AT1G61980 28.093 388 204 7 31 381 50 399 4.60e-36 137
MS.gene52435.t1 AT1G61980 28.093 388 204 7 31 381 50 399 4.60e-36 137
MS.gene52435.t1 AT1G61990 28.325 406 201 10 32 395 51 408 2.02e-33 130
MS.gene52435.t1 AT1G61990 28.325 406 201 10 32 395 51 408 2.02e-33 130
MS.gene52435.t1 AT1G61970 26.992 389 203 9 33 381 52 399 3.29e-31 124
MS.gene52435.t1 AT1G61970 26.992 389 203 9 33 381 52 399 3.29e-31 124
MS.gene52435.t1 AT1G61970 26.992 389 203 9 33 381 52 399 3.29e-31 124
MS.gene52435.t1 AT1G61970 26.992 389 203 9 33 381 52 399 3.29e-31 124
MS.gene52435.t1 AT1G61970 26.992 389 203 9 33 381 52 399 3.29e-31 124
MS.gene52435.t1 AT1G61970 26.992 389 203 9 33 381 52 399 3.29e-31 124
MS.gene52435.t1 AT1G61970 26.992 389 203 9 33 381 52 399 3.29e-31 124
MS.gene52435.t1 AT3G46950 28.000 300 173 4 15 313 34 291 3.68e-29 118
MS.gene52435.t1 AT1G62120 27.551 392 209 6 6 358 26 381 7.09e-28 114
MS.gene52435.t1 AT5G64950 25.474 369 216 8 44 395 49 375 9.66e-24 102
MS.gene52435.t1 AT1G62110 28.929 280 162 6 32 310 51 294 1.71e-23 102
MS.gene52435.t1 AT1G61960 29.032 279 160 3 33 310 52 293 3.89e-23 101
MS.gene52435.t1 AT1G62150 29.066 289 168 7 23 310 48 300 4.76e-23 101
MS.gene52435.t1 AT1G62085 29.032 279 161 3 33 310 55 297 3.42e-22 98.6
MS.gene52435.t1 AT1G62085 29.032 279 161 3 33 310 55 297 3.42e-22 98.6
MS.gene52435.t1 AT5G23930 26.371 383 244 9 32 381 54 431 2.73e-20 93.2
MS.gene52435.t1 AT1G56380 24.462 372 237 11 34 381 17 368 1.94e-16 80.9
MS.gene52435.t1 AT1G56380 24.462 372 237 11 34 381 28 379 2.62e-16 80.5
MS.gene52435.t1 AT1G62010 23.939 330 173 6 66 358 71 359 2.70e-15 77.8

Find 71 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCTTGATTTCACGAATTT+AGG 0.108304 2.3:+54343540 None:intergenic
TCCTACCTCATCCACAAATT+TGG 0.167245 2.3:-54343807 MS.gene52435:CDS
GTTAATTTGATGTCTGATTT+TGG 0.175734 2.3:-54343282 MS.gene52435:CDS
CATCCTTCAACGACTTCTTT+TGG 0.239296 2.3:-54343156 MS.gene52435:CDS
AAACTATAACCAAACATAAT+TGG 0.255041 2.3:+54342922 None:intergenic
TTGGAGGAAGTTAAGTGTTT+AGG 0.257551 2.3:-54343183 MS.gene52435:CDS
TTGATTTGTTGATGAGCTTT+TGG 0.261432 2.3:-54342987 MS.gene52435:CDS
AAGTCAAGGCCATCTATATT+TGG 0.261665 2.3:-54343228 MS.gene52435:CDS
TGGAGGAAGTTAAGTGTTTA+GGG 0.262883 2.3:-54343182 MS.gene52435:CDS
AAACTACCAATGACATTATT+AGG 0.279319 2.3:+54343255 None:intergenic
AATTTCTCAGAATAAGTAAA+TGG 0.313249 2.3:+54342814 None:intergenic
TTGGTTATAGTTTACATAAA+AGG 0.315414 2.3:-54342912 MS.gene52435:CDS
TTAAAGCTATATGACGAAAA+AGG 0.320094 2.3:-54342742 MS.gene52435:CDS
AAAGGATTTGATTAAGTCAT+TGG 0.323129 2.3:-54343202 MS.gene52435:CDS
AGCTTGATTTCACGAATTTA+GGG 0.334381 2.3:+54343541 None:intergenic
AAGAAGTTGATAACTGAATC+TGG 0.340602 2.3:+54343723 None:intergenic
GCAAATACTGATATCCTTGT+TGG 0.344981 2.3:+54343362 None:intergenic
AAGCTGTTATTCGAACATTT+AGG 0.358207 2.3:-54343047 MS.gene52435:CDS
ATATCAGTATTTGCTTAATT+CGG 0.363607 2.3:-54343353 MS.gene52435:CDS
AATTAACATTATCCACAATA+AGG 0.365879 2.3:+54343298 None:intergenic
GGTAGAGTGGTGTTGTTGAT+AGG 0.366564 2.3:+54343880 None:intergenic
ACTAAATGTCCAATTATGTT+TGG 0.386959 2.3:-54342931 MS.gene52435:CDS
AGATTAGTAAATTACACATT+TGG 0.386965 2.3:+54342678 None:intergenic
ATCAACTTCTTCAAAATTCA+CGG 0.390183 2.3:-54343711 MS.gene52435:CDS
TCCTTTGAACCAAATATAGA+TGG 0.398078 2.3:+54343219 None:intergenic
TGAAGAATCTGATAGAAAAT+TGG 0.398100 2.3:+54343859 None:intergenic
GGAGTTCCTAATAATGTCAT+TGG 0.406851 2.3:-54343261 MS.gene52435:CDS
GTCAGAGGAAGAAGCACCTT+TGG 0.409246 2.3:+54343587 None:intergenic
AAGCAACTTTGCAAAATTTG+AGG 0.414928 2.3:+54343435 None:intergenic
GCCATCTATATTTGGTTCAA+AGG 0.427799 2.3:-54343220 MS.gene52435:CDS
TTCCTCTGACATTGTTCGTA+TGG 0.429860 2.3:-54343574 MS.gene52435:CDS
TCAATTGGGTCGGAATTCCT+TGG 0.449002 2.3:-54342959 MS.gene52435:CDS
AGCAACTTTGCAAAATTTGA+GGG 0.450806 2.3:+54343436 None:intergenic
TTAGTAAATTACACATTTGG+TGG 0.452312 2.3:+54342681 None:intergenic
ACATACACCGCATCATCAAA+AGG 0.466920 2.3:-54343674 MS.gene52435:CDS
AAGAGAAACGGTTTGAACAT+TGG 0.491996 2.3:+54343918 None:intergenic
CTGCAATTTCTATCTATGAA+AGG 0.500353 2.3:-54342868 MS.gene52435:CDS
ATTGCTTTGGCAGCCAAGAA+AGG 0.500968 2.3:-54343132 MS.gene52435:CDS
ATCAGAGGAAGAAGCACCAT+TGG 0.505631 2.3:+54343482 None:intergenic
GAATTCTTATCACCTTATTG+TGG 0.506274 2.3:-54343310 MS.gene52435:CDS
CGAACATTTAGGTTGCATCC+TGG 0.509848 2.3:-54343036 MS.gene52435:CDS
ATTCCAAAAGAAGTCGTTGA+AGG 0.513854 2.3:+54343153 None:intergenic
TACATAAAAGGATCATTCCA+AGG 0.520767 2.3:-54342900 MS.gene52435:CDS
TTTCCTGCGATACCCACAAA+AGG 0.521045 2.3:-54343638 MS.gene52435:CDS
GTTGCATCCTGGTTTGATGT+TGG 0.522713 2.3:-54343025 MS.gene52435:CDS
ACAGCATTGCGGAAGAGAAA+CGG 0.528960 2.3:+54343906 None:intergenic
CTGATAGAAAATTGGTAGAG+TGG 0.530821 2.3:+54343867 None:intergenic
GGTATCGCAGGAAAGAAGCC+AGG 0.539957 2.3:+54343647 None:intergenic
GTAGAGTGGTGTTGTTGATA+GGG 0.540082 2.3:+54343881 None:intergenic
AATTGCAGAACTGAGGCCCT+TGG 0.541980 2.3:+54342883 None:intergenic
CATACACCGCATCATCAAAA+GGG 0.551684 2.3:-54343673 MS.gene52435:CDS
TGGACATTTAGTAAGTTCCA+AGG 0.554638 2.3:+54342942 None:intergenic
TTGGTAGTTTGCTTAAGTCA+AGG 0.561949 2.3:-54343242 MS.gene52435:CDS
TTTGGGTCAATCAATTGGGT+CGG 0.562496 2.3:-54342969 MS.gene52435:CDS
AAACAGTGAATGATTGAGAA+GGG 0.564998 2.3:+54343829 None:intergenic
AGAATCCAAATTTGTGGATG+AGG 0.569749 2.3:+54343802 None:intergenic
GGATTTGATTAAGTCATTGG+AGG 0.571043 2.3:-54343199 MS.gene52435:CDS
CATGTGAGAATCCAAATTTG+TGG 0.580478 2.3:+54343796 None:intergenic
TCCAAATTTGTGGATGAGGT+AGG 0.597396 2.3:+54343806 None:intergenic
GAAACAGTGAATGATTGAGA+AGG 0.600981 2.3:+54343828 None:intergenic
ACATAAAAGGATCATTCCAA+GGG 0.612722 2.3:-54342899 MS.gene52435:CDS
TAACAATGAAACAATATCAG+AGG 0.619380 2.3:+54343467 None:intergenic
AACAGTGAATGATTGAGAAG+GGG 0.629756 2.3:+54343830 None:intergenic
GCATCATCAAAAGGGAACCC+TGG 0.632776 2.3:-54343665 MS.gene52435:CDS
ATTGAAGCCAACATCAAACC+AGG 0.644090 2.3:+54343018 None:intergenic
AACCATACGAACAATGTCAG+AGG 0.658039 2.3:+54343572 None:intergenic
AGATAGAAATTGCAGAACTG+AGG 0.664204 2.3:+54342876 None:intergenic
TTGATAGGGCAACAGCATTG+CGG 0.672663 2.3:+54343895 None:intergenic
GCAAACACAAAGGAGAACAA+TGG 0.674249 2.3:-54342712 MS.gene52435:CDS
AGGCAAACTTGCAAACACAA+AGG 0.675272 2.3:-54342722 MS.gene52435:CDS
GTATCGCAGGAAAGAAGCCA+GGG 0.691083 2.3:+54343648 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTATAACCAAACATAAT+TGG + chr2.3:54343677-54343696 None:intergenic 20.0%
!! AATTAACATTATCCACAATA+AGG + chr2.3:54343301-54343320 None:intergenic 20.0%
!! AATTTCTCAGAATAAGTAAA+TGG + chr2.3:54343785-54343804 None:intergenic 20.0%
!! ATATCAGTATTTGCTTAATT+CGG - chr2.3:54343243-54343262 MS.gene52435:CDS 20.0%
!! TACTTAACAATTGAAAAAGA+GGG + chr2.3:54343202-54343221 None:intergenic 20.0%
!! TTGGTTATAGTTTACATAAA+AGG - chr2.3:54343684-54343703 MS.gene52435:CDS 20.0%
! AAACTACCAATGACATTATT+AGG + chr2.3:54343344-54343363 None:intergenic 25.0%
! AAAGGATTTGATTAAGTCAT+TGG - chr2.3:54343394-54343413 MS.gene52435:CDS 25.0%
! AAGGTTTGAGTAAAAAACTT+TGG - chr2.3:54343483-54343502 MS.gene52435:CDS 25.0%
! ACTAAATGTCCAATTATGTT+TGG - chr2.3:54343665-54343684 MS.gene52435:CDS 25.0%
! AGGTTTGAGTAAAAAACTTT+GGG - chr2.3:54343484-54343503 MS.gene52435:CDS 25.0%
! ATCAACTTCTTCAAAATTCA+CGG - chr2.3:54342885-54342904 MS.gene52435:CDS 25.0%
! CTACTTAACAATTGAAAAAG+AGG + chr2.3:54343203-54343222 None:intergenic 25.0%
! GAGAGTAAAAATTGAAACTT+TGG + chr2.3:54342990-54343009 None:intergenic 25.0%
! TAACAATGAAACAATATCAG+AGG + chr2.3:54343132-54343151 None:intergenic 25.0%
! TGAAGAATCTGATAGAAAAT+TGG + chr2.3:54342740-54342759 None:intergenic 25.0%
! TTAAAGCTATATGACGAAAA+AGG - chr2.3:54343854-54343873 MS.gene52435:CDS 25.0%
! TTAGTAAATTACACATTTGG+TGG + chr2.3:54343918-54343937 None:intergenic 25.0%
!! AGTTGATGCCTTTAAAAAAT+GGG - chr2.3:54343514-54343533 MS.gene52435:CDS 25.0%
!! TTTCAATTTTTACTCTCCAA+AGG - chr2.3:54342993-54343012 MS.gene52435:CDS 25.0%
!!! AAGTTGATGCCTTTAAAAAA+TGG - chr2.3:54343513-54343532 MS.gene52435:CDS 25.0%
!!! CTCTTTTTCAATTGTTAAGT+AGG - chr2.3:54343201-54343220 MS.gene52435:CDS 25.0%
!!! GTTAATTTGATGTCTGATTT+TGG - chr2.3:54343314-54343333 MS.gene52435:CDS 25.0%
!!! TTTCACTTTTTTCTATCCAA+TGG - chr2.3:54343098-54343117 MS.gene52435:CDS 25.0%
!!! TTTTTTACTCAAACCTTTCT+TGG + chr2.3:54343480-54343499 None:intergenic 25.0%
AAACAGTGAATGATTGAGAA+GGG + chr2.3:54342770-54342789 None:intergenic 30.0%
AAGCAACTTTGCAAAATTTG+AGG + chr2.3:54343164-54343183 None:intergenic 30.0%
AAGCTGTTATTCGAACATTT+AGG - chr2.3:54343549-54343568 MS.gene52435:CDS 30.0%
AAGCTTGATTTCACGAATTT+AGG + chr2.3:54343059-54343078 None:intergenic 30.0%
ACATAAAAGGATCATTCCAA+GGG - chr2.3:54343697-54343716 MS.gene52435:CDS 30.0%
AGCAACTTTGCAAAATTTGA+GGG + chr2.3:54343163-54343182 None:intergenic 30.0%
AGCTTGATTTCACGAATTTA+GGG + chr2.3:54343058-54343077 None:intergenic 30.0%
CTGCAATTTCTATCTATGAA+AGG - chr2.3:54343728-54343747 MS.gene52435:CDS 30.0%
GAATTCTTATCACCTTATTG+TGG - chr2.3:54343286-54343305 MS.gene52435:CDS 30.0%
TACATAAAAGGATCATTCCA+AGG - chr2.3:54343696-54343715 MS.gene52435:CDS 30.0%
TCCTTTGAACCAAATATAGA+TGG + chr2.3:54343380-54343399 None:intergenic 30.0%
! GTTGATGCCTTTAAAAAATG+GGG - chr2.3:54343515-54343534 MS.gene52435:CDS 30.0%
! TCAAATTTTGCAAAGTTGCT+TGG - chr2.3:54343163-54343182 MS.gene52435:CDS 30.0%
!! AAGAAGTTGATAACTGAATC+TGG + chr2.3:54342876-54342895 None:intergenic 30.0%
!! CAAGTTTGTTTTCTGCTTTA+AGG - chr2.3:54343814-54343833 MS.gene52435:CDS 30.0%
!! TAGGTTTTTGAAAACCAACA+AGG - chr2.3:54343220-54343239 MS.gene52435:CDS 30.0%
!!! GGTTTTGAAATAAAGCTGTT+TGG + chr2.3:54342844-54342863 None:intergenic 30.0%
!!! TGATTTGTTGATGAGCTTTT+GGG - chr2.3:54343610-54343629 MS.gene52435:CDS 30.0%
!!! TTGATTTGTTGATGAGCTTT+TGG - chr2.3:54343609-54343628 MS.gene52435:CDS 30.0%
AACAGTGAATGATTGAGAAG+GGG + chr2.3:54342769-54342788 None:intergenic 35.0%
AAGTCAAGGCCATCTATATT+TGG - chr2.3:54343368-54343387 MS.gene52435:CDS 35.0%
AGAATCCAAATTTGTGGATG+AGG + chr2.3:54342797-54342816 None:intergenic 35.0%
AGATAGAAATTGCAGAACTG+AGG + chr2.3:54343723-54343742 None:intergenic 35.0%
ATTCCAAAAGAAGTCGTTGA+AGG + chr2.3:54343446-54343465 None:intergenic 35.0%
CATGTGAGAATCCAAATTTG+TGG + chr2.3:54342803-54342822 None:intergenic 35.0%
CTGATAGAAAATTGGTAGAG+TGG + chr2.3:54342732-54342751 None:intergenic 35.0%
GAAACAGTGAATGATTGAGA+AGG + chr2.3:54342771-54342790 None:intergenic 35.0%
GCAAATACTGATATCCTTGT+TGG + chr2.3:54343237-54343256 None:intergenic 35.0%
GGAGTTCCTAATAATGTCAT+TGG - chr2.3:54343335-54343354 MS.gene52435:CDS 35.0%
GGATTTGATTAAGTCATTGG+AGG - chr2.3:54343397-54343416 MS.gene52435:CDS 35.0%
TGGACATTTAGTAAGTTCCA+AGG + chr2.3:54343657-54343676 None:intergenic 35.0%
TGGAGGAAGTTAAGTGTTTA+GGG - chr2.3:54343414-54343433 MS.gene52435:CDS 35.0%
TTGGAGGAAGTTAAGTGTTT+AGG - chr2.3:54343413-54343432 MS.gene52435:CDS 35.0%
! ACTTTGGTAGAATCCTTTTG+TGG + chr2.3:54342974-54342993 None:intergenic 35.0%
! ATGCCTTTAAAAAATGGGGT+TGG - chr2.3:54343519-54343538 MS.gene52435:CDS 35.0%
! CTTTGGTAGAATCCTTTTGT+GGG + chr2.3:54342973-54342992 None:intergenic 35.0%
! GCCATCTATATTTGGTTCAA+AGG - chr2.3:54343376-54343395 MS.gene52435:CDS 35.0%
!! AGCTTTTGGGTCAATCAATT+GGG - chr2.3:54343623-54343642 MS.gene52435:CDS 35.0%
!! TTGGTAGTTTGCTTAAGTCA+AGG - chr2.3:54343354-54343373 MS.gene52435:CDS 35.0%
!!! AACTGAATCTGGTTTTTGAG+AGG + chr2.3:54342865-54342884 None:intergenic 35.0%
!!! AGACCAACCCCATTTTTTAA+AGG + chr2.3:54343525-54343544 None:intergenic 35.0%
!!! GACTTCTTTTGGAATTGCTT+TGG - chr2.3:54343451-54343470 MS.gene52435:CDS 35.0%
!!! GTTTGTTTTCTGCTTTAAGG+AGG - chr2.3:54343817-54343836 MS.gene52435:CDS 35.0%
AACCATACGAACAATGTCAG+AGG + chr2.3:54343027-54343046 None:intergenic 40.0%
ACATACACCGCATCATCAAA+AGG - chr2.3:54342922-54342941 MS.gene52435:CDS 40.0%
AGGCAAACTTGCAAACACAA+AGG - chr2.3:54343874-54343893 MS.gene52435:CDS 40.0%
ATTGAAGCCAACATCAAACC+AGG + chr2.3:54343581-54343600 None:intergenic 40.0%
CATACACCGCATCATCAAAA+GGG - chr2.3:54342923-54342942 MS.gene52435:CDS 40.0%
GCAAACACAAAGGAGAACAA+TGG - chr2.3:54343884-54343903 MS.gene52435:CDS 40.0%
TCCAAATTTGTGGATGAGGT+AGG + chr2.3:54342793-54342812 None:intergenic 40.0%
TCCTACCTCATCCACAAATT+TGG - chr2.3:54342789-54342808 MS.gene52435:CDS 40.0%
TTCCTCTGACATTGTTCGTA+TGG - chr2.3:54343022-54343041 MS.gene52435:CDS 40.0%
TTTGGGTCAATCAATTGGGT+CGG - chr2.3:54343627-54343646 MS.gene52435:CDS 40.0%
! CATCCTTCAACGACTTCTTT+TGG - chr2.3:54343440-54343459 MS.gene52435:CDS 40.0%
!! GAGCTTTTGGGTCAATCAAT+TGG - chr2.3:54343622-54343641 MS.gene52435:CDS 40.0%
!! GTAGAGTGGTGTTGTTGATA+GGG + chr2.3:54342718-54342737 None:intergenic 40.0%
ACAGCATTGCGGAAGAGAAA+CGG + chr2.3:54342693-54342712 None:intergenic 45.0%
ATTGCTTTGGCAGCCAAGAA+AGG - chr2.3:54343464-54343483 MS.gene52435:CDS 45.0%
TCAATTGGGTCGGAATTCCT+TGG - chr2.3:54343637-54343656 MS.gene52435:CDS 45.0%
TTGATAGGGCAACAGCATTG+CGG + chr2.3:54342704-54342723 None:intergenic 45.0%
TTTCCTGCGATACCCACAAA+AGG - chr2.3:54342958-54342977 MS.gene52435:CDS 45.0%
! GTTGCATCCTGGTTTGATGT+TGG - chr2.3:54343571-54343590 MS.gene52435:CDS 45.0%
!! AATCCTTTTGTGGGTATCGC+AGG + chr2.3:54342964-54342983 None:intergenic 45.0%
!! ATCAGAGGAAGAAGCACCAT+TGG + chr2.3:54343117-54343136 None:intergenic 45.0%
!! CGAACATTTAGGTTGCATCC+TGG - chr2.3:54343560-54343579 MS.gene52435:CDS 45.0%
!! GGTAGAGTGGTGTTGTTGAT+AGG + chr2.3:54342719-54342738 None:intergenic 45.0%
!!! AGGGTTCCCTTTTGATGATG+CGG + chr2.3:54342932-54342951 None:intergenic 45.0%
AATTGCAGAACTGAGGCCCT+TGG + chr2.3:54343716-54343735 None:intergenic 50.0%
GCATCATCAAAAGGGAACCC+TGG - chr2.3:54342931-54342950 MS.gene52435:CDS 50.0%
GTATCGCAGGAAAGAAGCCA+GGG + chr2.3:54342951-54342970 None:intergenic 50.0%
!! GTCAGAGGAAGAAGCACCTT+TGG + chr2.3:54343012-54343031 None:intergenic 50.0%
GGTATCGCAGGAAAGAAGCC+AGG + chr2.3:54342952-54342971 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 54342681 54343937 54342681 ID=MS.gene52435
chr2.3 mRNA 54342681 54343937 54342681 ID=MS.gene52435.t1;Parent=MS.gene52435
chr2.3 exon 54342681 54343937 54342681 ID=MS.gene52435.t1.exon1;Parent=MS.gene52435.t1
chr2.3 CDS 54342681 54343937 54342681 ID=cds.MS.gene52435.t1;Parent=MS.gene52435.t1
Gene Sequence

>MS.gene52435

ATGTTCAAACCGTTTCTCTTCCGCAATGCTGTTGCCCTATCAACAACACCACTCTACCAATTTTCTATCAGATTCTTCAACACTGCCCCTTCTCAATCATTCACTGTTTCCTACCTCATCCACAAATTTGGATTCTCACATGAATTTGCACTCAAAGCTTCCAAACAGCTTTATTTCAAAACCTCTCAAAAACCAGATTCAGTTATCAACTTCTTCAAAATTCACGGTTTCACTGACTCAGACATACACCGCATCATCAAAAGGGAACCCTGGCTTCTTTCCTGCGATACCCACAAAAGGATTCTACCAAAGTTTCAATTTTTACTCTCCAAAGGTGCTTCTTCCTCTGACATTGTTCGTATGGTTGTAAGTAACCCTAAATTCGTGAAATCAAGCTTGAAAAATCATGAAATTATGTTTCACTTTTTTCTATCCAATGGTGCTTCTTCCTCTGATATTGTTTCATTGTTAACTGCAAACCCTCAAATTTTGCAAAGTTGCTTGGAGAAACGAATAATCCCTCTTTTTCAATTGTTAAGTAGGTTTTTGAAAACCAACAAGGATATCAGTATTTGCTTAATTCGGCATTCGACTACATTTTCTATGAATTCTTATCACCTTATTGTGGATAATGTTAATTTGATGTCTGATTTTGGAGTTCCTAATAATGTCATTGGTAGTTTGCTTAAGTCAAGGCCATCTATATTTGGTTCAAAGGATTTGATTAAGTCATTGGAGGAAGTTAAGTGTTTAGGGTTTCATCCTTCAACGACTTCTTTTGGAATTGCTTTGGCAGCCAAGAAAGGTTTGAGTAAAAAACTTTGGGATGAGAAAGTTGATGCCTTTAAAAAATGGGGTTGGTCTGATGAAGCTGTTATTCGAACATTTAGGTTGCATCCTGGTTTGATGTTGGCTTCAATTGATAAGATTGATTTGTTGATGAGCTTTTGGGTCAATCAATTGGGTCGGAATTCCTTGGAACTTACTAAATGTCCAATTATGTTTGGTTATAGTTTACATAAAAGGATCATTCCAAGGGCCTCAGTTCTGCAATTTCTATCTATGAAAGGTTTGCGAAAAAAGAATGCAAGCTTAGTTAAACCATTTACTTATTCTGAGAAATTGTTCTTGAGCAAGTTTGTTTTCTGCTTTAAGGAGGAGTCTGATTATCTATTAAAGCTATATGACGAAAAAGGCAAACTTGCAAACACAAAGGAGAACAATGGCATGCTGTCCACCAAATGTGTAATTTACTAA
Protein sequence

>MS.gene52435.t1

MFKPFLFRNAVALSTTPLYQFSIRFFNTAPSQSFTVSYLIHKFGFSHEFALKASKQLYFKTSQKPDSVINFFKIHGFTDSDIHRIIKREPWLLSCDTHKRILPKFQFLLSKGASSSDIVRMVVSNPKFVKSSLKNHEIMFHFFLSNGASSSDIVSLLTANPQILQSCLEKRIIPLFQLLSRFLKTNKDISICLIRHSTTFSMNSYHLIVDNVNLMSDFGVPNNVIGSLLKSRPSIFGSKDLIKSLEEVKCLGFHPSTTSFGIALAAKKGLSKKLWDEKVDAFKKWGWSDEAVIRTFRLHPGLMLASIDKIDLLMSFWVNQLGRNSLELTKCPIMFGYSLHKRIIPRASVLQFLSMKGLRKKNASLVKPFTYSEKLFLSKFVFCFKEESDYLLKLYDEKGKLANTKENNGMLSTKCVIY