AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52475

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52475.t1 MTR_7g037260 95.706 163 7 0 1 163 195 357 6.22e-110 317
MS.gene52475.t1 MTR_7g037130 91.411 163 14 0 1 163 195 357 1.09e-105 306
MS.gene52475.t1 MTR_7g451170 73.099 171 38 1 1 163 241 411 6.30e-83 250
MS.gene52475.t1 MTR_4g088015 70.760 171 42 1 1 163 271 441 1.90e-74 229
MS.gene52475.t1 MTR_7g035350 53.529 170 56 4 1 148 275 443 2.64e-48 162
MS.gene52475.t1 MTR_1g057980 53.614 166 58 4 1 148 130 294 1.61e-44 148
MS.gene52475.t1 MTR_7g450950 55.714 140 44 4 1 138 207 330 3.87e-38 133
MS.gene52475.t1 MTR_7g035300 41.104 163 52 3 1 163 114 232 8.04e-31 111
MS.gene52475.t1 MTR_3g077650 69.014 71 22 0 1 71 231 301 2.56e-28 108
MS.gene52475.t1 MTR_4g105660 37.056 197 83 8 1 160 230 422 4.60e-28 108
MS.gene52475.t1 MTR_3g463400 51.852 81 34 2 1 76 183 263 3.14e-23 95.1
MS.gene52475.t1 MTR_5g070020 54.667 75 32 1 1 73 172 246 3.56e-23 94.0
MS.gene52475.t1 MTR_5g049190 35.632 174 96 5 1 161 69 239 5.33e-23 91.7
MS.gene52475.t1 MTR_4g019370 58.824 51 21 0 1 51 88 138 7.93e-17 76.6
MS.gene52475.t1 MTR_1g026870 52.941 51 24 0 1 51 135 185 4.85e-15 72.0
MS.gene52475.t1 MTR_3g110028 50.980 51 25 0 1 51 135 185 6.22e-15 71.6
MS.gene52475.t1 MTR_7g061330 54.902 51 23 0 1 51 163 213 9.50e-15 71.2
MS.gene52475.t1 MTR_7g461410 54.902 51 23 0 1 51 163 213 9.50e-15 71.2
MS.gene52475.t1 MTR_7g061330 54.902 51 23 0 1 51 127 177 1.34e-14 70.5
MS.gene52475.t1 MTR_4g073420 54.237 59 24 1 1 59 65 120 3.03e-14 68.9
MS.gene52475.t1 MTR_8g095390 50.980 51 25 0 1 51 65 115 8.20e-14 67.8
MS.gene52475.t1 MTR_5g010650 60.000 45 18 0 1 45 160 204 2.71e-13 67.0
MS.gene52475.t1 MTR_3g097450 50.980 51 25 0 1 51 65 115 4.61e-13 65.5
MS.gene52475.t1 MTR_5g041570 47.368 57 30 0 1 57 184 240 5.57e-13 65.9
MS.gene52475.t1 MTR_5g082910 61.905 42 16 0 6 47 74 115 5.67e-13 65.5
MS.gene52475.t1 MTR_2g064160 49.020 51 26 0 1 51 64 114 6.11e-13 65.5
MS.gene52475.t1 MTR_5g029840 46.296 54 29 0 1 54 65 118 9.49e-13 65.1
MS.gene52475.t1 MTR_3g052450 52.830 53 24 1 1 52 93 145 1.27e-12 65.1
MS.gene52475.t1 MTR_4g094982 50.980 51 25 0 1 51 55 105 1.69e-12 63.9
MS.gene52475.t1 MTR_6g009430 48.148 54 28 0 1 54 65 118 2.01e-12 63.9
MS.gene52475.t1 MTR_3g052430 50.943 53 25 1 1 52 94 146 2.75e-12 63.9
MS.gene52475.t1 MTR_8g042410 50.000 54 26 1 1 53 90 143 2.81e-12 63.9
MS.gene52475.t1 MTR_5g016510 50.980 51 25 0 1 51 55 105 3.21e-12 63.5
MS.gene52475.t1 MTR_8g017440 45.098 51 28 0 1 51 65 115 6.34e-12 62.8
MS.gene52475.t1 MTR_8g017340 49.020 51 26 0 1 51 66 116 6.89e-12 62.8
MS.gene52475.t1 MTR_3g101290 47.170 53 28 0 1 53 87 139 7.30e-12 61.6
MS.gene52475.t1 MTR_4g082040 41.791 67 34 1 1 62 155 221 7.40e-12 62.8
MS.gene52475.t1 MTR_4g091490 49.020 51 26 0 1 51 66 116 7.67e-12 62.4
MS.gene52475.t1 MTR_8g017540 45.098 51 28 0 1 51 111 161 8.02e-12 62.4
MS.gene52475.t1 MTR_4g485530 36.170 94 56 1 1 90 65 158 8.60e-12 60.8
MS.gene52475.t1 MTR_8g031360 30.709 127 66 3 1 108 67 190 9.63e-12 62.0
MS.gene52475.t1 MTR_6g015455 50.980 51 25 0 1 51 55 105 9.86e-12 62.4
MS.gene52475.t1 MTR_2g011660 49.020 51 26 0 1 51 66 116 1.02e-11 62.0
MS.gene52475.t1 MTR_3g028740 47.170 53 27 1 1 52 75 127 1.19e-11 62.0
MS.gene52475.t1 MTR_4g063100 40.678 59 35 0 1 59 31 89 1.30e-11 60.8
MS.gene52475.t1 MTR_7g076740 50.000 54 26 1 1 53 66 119 1.30e-11 61.6
MS.gene52475.t1 MTR_8g017390 40.351 57 34 0 1 57 66 122 1.31e-11 62.0
MS.gene52475.t1 MTR_4g063100 43.396 53 30 0 7 59 2 54 1.34e-11 60.1
MS.gene52475.t1 MTR_5g079290 45.455 55 30 0 1 55 70 124 1.49e-11 61.2
MS.gene52475.t1 MTR_7g110830 44.643 56 28 1 1 53 65 120 1.69e-11 61.6
MS.gene52475.t1 MTR_2g099740 45.098 51 28 0 1 51 65 115 1.80e-11 61.6
MS.gene52475.t1 MTR_8g027345 52.174 46 22 0 1 46 65 110 1.85e-11 61.2
MS.gene52475.t1 MTR_6g055910 28.994 169 86 8 8 151 5 164 2.06e-11 60.1
MS.gene52475.t1 MTR_2g097910 45.283 53 28 1 1 52 73 125 2.11e-11 61.2
MS.gene52475.t1 MTR_2g089620 47.059 51 27 0 1 51 65 115 2.16e-11 61.2
MS.gene52475.t1 MTR_6g074860 41.429 70 38 1 1 67 54 123 2.46e-11 60.5
MS.gene52475.t1 MTR_1g085640 38.182 55 34 0 1 55 65 119 2.50e-11 61.2
MS.gene52475.t1 MTR_4g063100 42.857 56 32 0 1 56 60 115 2.58e-11 60.5
MS.gene52475.t1 MTR_5g078910 49.020 51 26 0 1 51 60 110 2.67e-11 60.8
MS.gene52475.t1 MTR_3g011610 48.148 54 27 1 1 53 90 143 2.86e-11 60.8
MS.gene52475.t1 MTR_4g102380 46.296 54 29 0 1 54 65 118 2.89e-11 60.5
MS.gene52475.t1 MTR_0008s0390 47.059 51 27 0 1 51 66 116 2.97e-11 60.5
MS.gene52475.t1 MTR_5g079220 43.636 55 31 0 1 55 74 128 3.08e-11 60.5
MS.gene52475.t1 MTR_1g043050 28.571 161 96 5 1 152 65 215 3.15e-11 60.1
MS.gene52475.t1 MTR_4g097570 27.097 155 105 2 1 153 66 214 3.23e-11 60.5
MS.gene52475.t1 MTR_4g097570 27.097 155 105 2 1 153 67 215 3.28e-11 60.5
MS.gene52475.t1 MTR_4g125520 28.221 163 84 5 1 145 65 212 3.48e-11 60.5
MS.gene52475.t1 MTR_4g478180 47.059 51 27 0 1 51 66 116 3.82e-11 60.5
MS.gene52475.t1 MTR_6g055910 28.994 169 86 8 8 151 46 205 4.04e-11 59.7
MS.gene52475.t1 MTR_5g078860 49.020 51 26 0 1 51 60 110 4.93e-11 60.1
MS.gene52475.t1 MTR_7g117730 52.174 46 22 0 1 46 65 110 5.70e-11 59.3
MS.gene52475.t1 MTR_6g055910 28.481 158 81 7 8 140 75 225 5.76e-11 59.7
MS.gene52475.t1 MTR_1g085040 39.216 51 31 0 1 51 65 115 5.96e-11 60.1
MS.gene52475.t1 MTR_6g090405 41.429 70 37 1 1 70 66 131 6.38e-11 59.3
MS.gene52475.t1 MTR_5g079670 44.444 54 30 0 1 54 64 117 6.84e-11 58.5
MS.gene52475.t1 MTR_8g017350 47.059 51 27 0 1 51 66 116 6.88e-11 60.1
MS.gene52475.t1 MTR_7g017260 44.643 56 31 0 1 56 80 135 6.89e-11 59.3
MS.gene52475.t1 MTR_4g065017 45.283 53 28 1 1 52 70 122 7.58e-11 59.7
MS.gene52475.t1 MTR_0008s0470 45.098 51 28 0 1 51 66 116 9.62e-11 58.9
MS.gene52475.t1 MTR_1g100653 45.098 51 28 0 1 51 65 115 9.80e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52475.t1 AT4G18770 64.789 71 25 0 1 71 267 337 6.60e-27 105
MS.gene52475.t1 AT5G40360 58.442 77 30 1 1 75 208 284 3.86e-23 94.0
MS.gene52475.t1 AT2G25230 54.667 75 34 0 1 75 76 150 1.74e-22 90.1
MS.gene52475.t1 AT3G27785 58.228 79 29 2 1 75 239 317 2.79e-22 92.4
MS.gene52475.t1 AT5G58850 37.500 144 71 4 1 127 155 296 4.85e-21 89.0
MS.gene52475.t1 AT5G11050 62.069 58 22 0 1 58 155 212 1.75e-20 87.0
MS.gene52475.t1 AT5G40430 38.406 138 64 3 1 125 103 232 4.15e-20 84.3
MS.gene52475.t1 AT1G57560 29.697 165 95 4 1 157 65 216 5.19e-15 71.2
MS.gene52475.t1 AT5G17800 33.088 136 83 2 1 136 143 270 7.66e-15 70.9
MS.gene52475.t1 AT5G14750 31.092 119 81 1 1 119 8 125 1.09e-14 67.4
MS.gene52475.t1 AT5G14750 31.092 119 81 1 1 119 8 125 1.09e-14 67.4
MS.gene52475.t1 AT1G09540 29.530 149 93 4 1 146 65 204 1.49e-14 70.1
MS.gene52475.t1 AT3G55730 49.153 59 30 0 1 59 106 164 1.52e-14 70.5
MS.gene52475.t1 AT5G02320 28.736 174 100 6 1 161 177 339 2.07e-14 70.1
MS.gene52475.t1 AT5G02320 28.736 174 100 6 1 161 177 339 2.07e-14 70.1
MS.gene52475.t1 AT5G02320 28.736 174 100 6 1 161 117 279 2.46e-14 69.7
MS.gene52475.t1 AT5G14750 31.356 118 80 1 1 118 35 151 3.06e-14 67.0
MS.gene52475.t1 AT4G09460 30.822 146 91 2 1 139 65 207 3.37e-14 68.2
MS.gene52475.t1 AT4G38620 54.237 59 24 1 1 59 65 120 3.86e-14 68.6
MS.gene52475.t1 AT3G09230 45.588 68 37 0 1 68 105 172 4.43e-14 68.9
MS.gene52475.t1 AT4G32730 50.980 51 25 0 1 51 137 187 6.38e-14 68.9
MS.gene52475.t1 AT4G32730 50.980 51 25 0 1 51 137 187 6.38e-14 68.9
MS.gene52475.t1 AT4G32730 50.980 51 25 0 1 51 137 187 6.38e-14 68.9
MS.gene52475.t1 AT4G32730 50.980 51 25 0 1 51 137 187 6.38e-14 68.9
MS.gene52475.t1 AT5G14750 31.092 119 81 1 1 119 69 186 7.98e-14 66.6
MS.gene52475.t1 AT2G32460 55.556 54 23 1 1 53 71 124 8.12e-14 68.6
MS.gene52475.t1 AT2G32460 55.556 54 23 1 1 53 59 112 8.45e-14 68.2
MS.gene52475.t1 AT4G32730 50.980 51 25 0 1 51 137 187 8.99e-14 68.6
MS.gene52475.t1 AT1G22640 46.032 63 34 0 1 63 8 70 8.99e-14 66.2
MS.gene52475.t1 AT5G11510 47.059 51 27 0 1 51 131 181 1.33e-13 67.8
MS.gene52475.t1 AT5G11510 47.059 51 27 0 1 51 131 181 1.34e-13 67.8
MS.gene52475.t1 AT5G11510 47.059 51 27 0 1 51 131 181 1.38e-13 67.8
MS.gene52475.t1 AT5G11510 47.059 51 27 0 1 51 131 181 1.38e-13 67.8
MS.gene52475.t1 AT5G11510 47.059 51 27 0 1 51 131 181 1.38e-13 67.8
MS.gene52475.t1 AT3G28470 52.174 46 22 0 1 46 65 110 1.57e-13 67.0
MS.gene52475.t1 AT3G09370 50.980 51 25 0 1 51 193 243 3.23e-13 66.6
MS.gene52475.t1 AT3G11440 50.000 54 26 1 1 53 94 147 3.49e-13 66.6
MS.gene52475.t1 AT3G09370 50.980 51 25 0 1 51 185 235 3.61e-13 66.6
MS.gene52475.t1 AT3G09370 50.980 51 25 0 1 51 180 230 3.74e-13 66.6
MS.gene52475.t1 AT3G11440 50.000 54 26 1 1 53 94 147 3.82e-13 66.6
MS.gene52475.t1 AT3G11440 50.000 54 26 1 1 53 94 147 3.82e-13 66.6
MS.gene52475.t1 AT3G11440 50.000 54 26 1 1 53 94 147 3.82e-13 66.6
MS.gene52475.t1 AT1G22640 52.941 51 24 0 1 51 65 115 4.06e-13 65.5
MS.gene52475.t1 AT3G12720 49.020 51 26 0 1 51 75 125 4.47e-13 65.9
MS.gene52475.t1 AT1G69560 40.278 72 43 0 1 72 173 244 8.53e-13 65.1
MS.gene52475.t1 AT4G34990 49.091 55 28 0 1 55 65 119 9.45e-13 64.7
MS.gene52475.t1 AT1G69560 40.278 72 43 0 1 72 157 228 1.10e-12 64.7
MS.gene52475.t1 AT2G39880 43.636 55 31 0 1 55 100 154 1.12e-12 65.1
MS.gene52475.t1 AT1G57560 28.025 157 69 4 1 157 65 177 1.16e-12 64.3
MS.gene52475.t1 AT4G00540 55.102 49 22 0 3 51 158 206 1.33e-12 64.7
MS.gene52475.t1 AT5G06100 48.148 54 27 1 1 53 85 138 1.42e-12 64.7
MS.gene52475.t1 AT5G06100 48.148 54 27 1 1 53 85 138 1.52e-12 64.7
MS.gene52475.t1 AT5G06100 48.148 54 27 1 1 53 85 138 1.52e-12 64.7
MS.gene52475.t1 AT5G06100 48.148 54 27 1 1 53 85 138 1.52e-12 64.7
MS.gene52475.t1 AT5G06100 48.148 54 27 1 1 53 85 138 1.52e-12 64.7
MS.gene52475.t1 AT4G00540 55.102 49 22 0 3 51 156 204 1.75e-12 64.7
MS.gene52475.t1 AT1G17950 42.029 69 37 1 1 66 55 123 1.80e-12 63.5
MS.gene52475.t1 AT4G28110 46.296 54 29 0 1 54 65 118 2.34e-12 63.5
MS.gene52475.t1 AT1G26780 50.980 51 25 0 1 51 148 198 2.88e-12 63.9
MS.gene52475.t1 AT2G16720 49.020 51 26 0 1 51 65 115 3.05e-12 63.2
MS.gene52475.t1 AT2G26950 46.296 54 29 0 1 54 69 122 3.05e-12 63.5
MS.gene52475.t1 AT1G26780 50.980 51 25 0 1 51 148 198 3.34e-12 63.2
MS.gene52475.t1 AT3G53200 42.857 70 33 2 7 70 35 103 6.25e-12 61.6
MS.gene52475.t1 AT4G26930 48.000 50 26 0 1 50 72 121 6.55e-12 62.8
MS.gene52475.t1 AT3G27920 52.174 46 22 0 1 46 67 112 6.99e-12 61.6
MS.gene52475.t1 AT3G13890 45.098 51 28 0 1 51 74 124 7.95e-12 62.4
MS.gene52475.t1 AT3G13890 45.098 51 28 0 1 51 65 115 7.95e-12 62.4
MS.gene52475.t1 AT4G05100 48.148 54 28 0 1 54 66 119 9.73e-12 62.0
MS.gene52475.t1 AT1G18570 47.273 55 29 0 1 55 66 120 1.20e-11 62.0
MS.gene52475.t1 AT2G26960 52.000 50 24 0 1 50 73 122 1.22e-11 62.0
MS.gene52475.t1 AT5G16770 49.020 51 26 0 1 51 65 115 1.35e-11 61.6
MS.gene52475.t1 AT5G16770 49.020 51 26 0 1 51 65 115 1.35e-11 61.6
MS.gene52475.t1 AT3G53200 42.857 70 33 2 7 70 68 136 1.36e-11 60.8
MS.gene52475.t1 AT5G12870 42.593 54 31 0 1 54 71 124 1.43e-11 61.2
MS.gene52475.t1 AT5G67300 45.763 59 32 0 1 59 56 114 1.50e-11 61.6
MS.gene52475.t1 AT5G16770 49.020 51 26 0 1 51 65 115 1.66e-11 61.6
MS.gene52475.t1 AT5G16770 49.020 51 26 0 1 51 65 115 1.66e-11 61.6
MS.gene52475.t1 AT5G26660 40.000 55 33 0 1 55 34 88 1.75e-11 61.2
MS.gene52475.t1 AT3G08500 47.170 53 27 1 1 52 83 135 2.06e-11 61.2
MS.gene52475.t1 AT4G12350 42.593 54 31 0 1 54 65 118 2.22e-11 60.8
MS.gene52475.t1 AT5G35550 39.655 58 35 0 1 58 70 127 2.22e-11 60.8
MS.gene52475.t1 AT3G23250 38.298 94 51 4 1 88 8 100 2.56e-11 60.1
MS.gene52475.t1 AT5G40330 55.000 40 18 0 7 46 71 110 3.08e-11 59.7
MS.gene52475.t1 AT4G33450 50.000 52 26 0 1 52 69 120 3.12e-11 60.1
MS.gene52475.t1 AT5G59780 46.000 50 27 0 7 56 2 51 3.39e-11 58.9
MS.gene52475.t1 AT2G23290 50.980 51 25 0 1 51 63 113 3.47e-11 60.5
MS.gene52475.t1 AT1G35515 45.763 59 29 1 1 59 65 120 3.54e-11 59.7
MS.gene52475.t1 AT5G26660 40.000 55 33 0 1 55 65 119 3.70e-11 60.5
MS.gene52475.t1 AT4G37260 47.059 51 27 0 1 51 63 113 3.91e-11 60.5
MS.gene52475.t1 AT5G59780 42.857 56 32 0 1 56 40 95 4.54e-11 59.3
MS.gene52475.t1 AT4G21440 35.789 95 59 1 1 93 65 159 4.60e-11 60.5
MS.gene52475.t1 AT3G48920 30.172 116 72 2 1 116 71 177 4.69e-11 59.7
MS.gene52475.t1 AT4G22680 44.444 54 30 0 1 54 65 118 5.21e-11 59.7
MS.gene52475.t1 AT5G55020 48.000 50 26 0 1 50 79 128 6.40e-11 60.1
MS.gene52475.t1 AT3G30210 33.594 128 67 4 1 116 80 201 6.58e-11 59.3
MS.gene52475.t1 AT3G23250 38.298 94 51 4 1 88 65 157 6.93e-11 59.3
MS.gene52475.t1 AT3G61250 45.098 51 28 0 1 51 65 115 7.06e-11 59.7
MS.gene52475.t1 AT3G46130 46.000 50 27 0 7 56 2 51 7.07e-11 58.5
MS.gene52475.t1 AT5G52600 47.458 59 25 1 1 53 65 123 7.35e-11 58.5
MS.gene52475.t1 AT4G01680 39.216 51 31 0 1 51 77 127 8.25e-11 59.7
MS.gene52475.t1 AT5G35550 39.655 58 35 0 1 58 67 124 8.59e-11 58.9
MS.gene52475.t1 AT5G59780 42.857 56 32 0 1 56 61 116 8.84e-11 58.9
MS.gene52475.t1 AT3G02940 47.059 51 27 0 1 51 65 115 8.98e-11 59.3

Find 45 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCTTCTTCTTGGCTTTC+TGG 0.037710 7.2:+61190535 None:intergenic
CTCTCAGAACTTTCATAATT+TGG 0.107846 7.2:+61190393 None:intergenic
TCCTGCTTTATAGGAATTTC+AGG 0.183391 7.2:+61190249 None:intergenic
AGGTCAATTTCCTGCTTTAT+AGG 0.200831 7.2:+61190240 None:intergenic
GTTACCCCGATTCTTCTTCT+TGG 0.263788 7.2:+61190527 None:intergenic
TAGCCACCAATTTCATCTTC+TGG 0.310919 7.2:+61190342 None:intergenic
ACTACTCCAGAAGATGAAAT+TGG 0.319338 7.2:-61190348 MS.gene52475:CDS
GGCTTTCTGGCGGCGCTTAG+TGG 0.332385 7.2:+61190548 None:intergenic
TCTCGGAGCATGATGAATAT+CGG 0.380696 7.2:-61190423 MS.gene52475:CDS
GATGGAATGGCAAGTGGGTT+TGG 0.392878 7.2:-61190294 MS.gene52475:CDS
GCAAGTGGGTTTGGATCAAA+TGG 0.421916 7.2:-61190285 MS.gene52475:CDS
GTGGTTCTTGACAGAGTTCT+CGG 0.425204 7.2:+61190578 None:intergenic
CCCTGAAATTCCTATAAAGC+AGG 0.433661 7.2:-61190250 MS.gene52475:CDS
AGAAATTGCAAGGAGATTGC+CGG 0.434643 7.2:-61190607 MS.gene52475:CDS
TCATCATCATTGAACATCAT+TGG 0.444693 7.2:+61190315 None:intergenic
AGAAAGCCAAGAAGAAGAAT+CGG 0.447063 7.2:-61190533 MS.gene52475:CDS
CTCGGAGCATGATGAATATC+GGG 0.466743 7.2:-61190422 MS.gene52475:CDS
TTCTGCTACGAAAGTTGAAA+AGG 0.470660 7.2:-61190460 MS.gene52475:CDS
AAGATATATAGAAACTCTTA+AGG 0.471628 7.2:-61190210 None:intergenic
CCTGCTTTATAGGAATTTCA+GGG 0.476507 7.2:+61190250 None:intergenic
GAAATTGCAAGGAGATTGCC+GGG 0.477484 7.2:-61190606 MS.gene52475:CDS
AGGAGATGATGAATAATTCT+CGG 0.487334 7.2:-61190440 MS.gene52475:CDS
GAAAGCCAAGAAGAAGAATC+GGG 0.489419 7.2:-61190532 MS.gene52475:CDS
ATCGAAGCTCACAAGATAGT+TGG 0.496519 7.2:-61190642 MS.gene52475:CDS
AATGATTGAAGAAAGAGTCA+TGG 0.514007 7.2:-61190689 MS.gene52475:intron
AAAGCAGGAAATTGACCTCA+TGG 0.534744 7.2:-61190235 MS.gene52475:CDS
AGAACTCTGTCAAGAACCAC+TGG 0.551331 7.2:-61190575 MS.gene52475:CDS
GAATCGGGGTAACAGTTCAA+AGG 0.553014 7.2:-61190517 MS.gene52475:CDS
ACAGAGTTCTCGGTGCGCCC+CGG 0.553736 7.2:+61190588 None:intergenic
GGCGCTTAGTGGTGTTCCAG+TGG 0.556964 7.2:+61190559 None:intergenic
AATCGGGGTAACAGTTCAAA+GGG 0.564615 7.2:-61190516 MS.gene52475:CDS
TGGGTTTGGATCAAATGGAA+TGG 0.578195 7.2:-61190280 MS.gene52475:CDS
GTTCAATGATGATGATGGAA+TGG 0.583601 7.2:-61190307 MS.gene52475:CDS
ACAAGATAGTTGGTAACAAG+TGG 0.585557 7.2:-61190632 MS.gene52475:CDS
ATGATGTTCAATGATGATGA+TGG 0.603439 7.2:-61190312 MS.gene52475:CDS
ACTCCAGAAGATGAAATTGG+TGG 0.615237 7.2:-61190345 MS.gene52475:CDS
ATGATGATGGAATGGCAAGT+GGG 0.639684 7.2:-61190299 MS.gene52475:CDS
CAAGATAGTTGGTAACAAGT+GGG 0.641772 7.2:-61190631 MS.gene52475:CDS
TTCTATATATCTTCTCCATG+AGG 0.643428 7.2:+61190220 None:intergenic
GATGATGATGGAATGGCAAG+TGG 0.644418 7.2:-61190300 MS.gene52475:CDS
GAAAGAGTCATGGACCGAAG+AGG 0.655361 7.2:-61190679 MS.gene52475:CDS
ACAAGTGGGAAGAAATTGCA+AGG 0.658551 7.2:-61190617 MS.gene52475:CDS
TCTTCTTCTTGGCTTTCTGG+CGG 0.705181 7.2:+61190538 None:intergenic
AAAGCCAAGAAGAAGAATCG+GGG 0.727248 7.2:-61190531 MS.gene52475:CDS
AAATTGCAAGGAGATTGCCG+GGG 0.787081 7.2:-61190605 MS.gene52475:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTATCTAAATATATTATAT+AGG - chr7.2:61190623-61190642 MS.gene52475:CDS 10.0%
!! ATTAAATTAATTCACCAATT+TGG + chr7.2:61190838-61190857 None:intergenic 15.0%
!! CATTCAAAAAAGTTAATAAA+TGG + chr7.2:61190879-61190898 None:intergenic 15.0%
!! TATAATTAAAATAGTGTACA+AGG - chr7.2:61190781-61190800 MS.gene52475:intron 15.0%
!!! TAAGTTATTAATTTTACGTT+TGG - chr7.2:61190526-61190545 MS.gene52475:CDS 15.0%
!!! ACTACTTTTGAAATACATAA+TGG - chr7.2:61191094-61191113 MS.gene52475:intron 20.0%
!!! TACAACTCATTTTAAAACTA+GGG - chr7.2:61190323-61190342 MS.gene52475:CDS 20.0%
!!! TTACAACTCATTTTAAAACT+AGG - chr7.2:61190322-61190341 MS.gene52475:CDS 20.0%
! AGGTTATGATTCTATAAGAT+AGG + chr7.2:61190277-61190296 None:intergenic 25.0%
! GGTTATGATTCTATAAGATA+GGG + chr7.2:61190276-61190295 None:intergenic 25.0%
!! AAATTTTGTCAATGTCACAA+CGG + chr7.2:61190585-61190604 None:intergenic 25.0%
AAACAAAACTTCAGATTGCT+TGG - chr7.2:61190728-61190747 MS.gene52475:intron 30.0%
AAGGATGCAAAACTTTACTT+GGG - chr7.2:61190800-61190819 MS.gene52475:intron 30.0%
AATGATTGAAGAAAGAGTCA+TGG - chr7.2:61190895-61190914 MS.gene52475:intron 30.0%
ACTCGTGATGTATATAACAT+CGG + chr7.2:61190685-61190704 None:intergenic 30.0%
AGGAGATGATGAATAATTCT+CGG - chr7.2:61191144-61191163 MS.gene52475:intron 30.0%
CTCTCAGAACTTTCATAATT+TGG + chr7.2:61191194-61191213 None:intergenic 30.0%
GGAATAAGCTATTACCAAAT+TGG - chr7.2:61190821-61190840 MS.gene52475:intron 30.0%
TCACGAGTATTATACATGAA+TGG - chr7.2:61190697-61190716 MS.gene52475:intron 30.0%
TCATCATCATTGAACATCAT+TGG + chr7.2:61191272-61191291 None:intergenic 30.0%
TTCTATATATCTTCTCCATG+AGG + chr7.2:61191367-61191386 None:intergenic 30.0%
! ATGATGTTCAATGATGATGA+TGG - chr7.2:61191272-61191291 MS.gene52475:intron 30.0%
! CAATATTTTGTCTTCCTCTT+CGG + chr7.2:61190922-61190941 None:intergenic 30.0%
!! TCTAAAGTTTGTTACAGTAG+AGG + chr7.2:61190297-61190316 None:intergenic 30.0%
ACTACTCCAGAAGATGAAAT+TGG - chr7.2:61191236-61191255 MS.gene52475:intron 35.0%
AGAAAGCCAAGAAGAAGAAT+CGG - chr7.2:61191051-61191070 MS.gene52475:intron 35.0%
AGGTCAATTTCCTGCTTTAT+AGG + chr7.2:61191347-61191366 None:intergenic 35.0%
CAAGGATGCAAAACTTTACT+TGG - chr7.2:61190799-61190818 MS.gene52475:intron 35.0%
CCTGCTTTATAGGAATTTCA+GGG + chr7.2:61191337-61191356 None:intergenic 35.0%
CTAGGGTTTCTAATAACTTG+TGG - chr7.2:61190340-61190359 MS.gene52475:CDS 35.0%
CTCGTGATGTATATAACATC+GGG + chr7.2:61190684-61190703 None:intergenic 35.0%
TCCTGCTTTATAGGAATTTC+AGG + chr7.2:61191338-61191357 None:intergenic 35.0%
TTCTGCTACGAAAGTTGAAA+AGG - chr7.2:61191124-61191143 MS.gene52475:intron 35.0%
!! ACAAGATAGTTGGTAACAAG+TGG - chr7.2:61190952-61190971 MS.gene52475:intron 35.0%
!! CAAGATAGTTGGTAACAAGT+GGG - chr7.2:61190953-61190972 MS.gene52475:intron 35.0%
!! GTTCAATGATGATGATGGAA+TGG - chr7.2:61191277-61191296 MS.gene52475:intron 35.0%
!!! GAGAGTGATTTTTTCTCTGA+AGG - chr7.2:61191209-61191228 MS.gene52475:intron 35.0%
AAAGCAGGAAATTGACCTCA+TGG - chr7.2:61191349-61191368 MS.gene52475:intron 40.0%
AAAGCCAAGAAGAAGAATCG+GGG - chr7.2:61191053-61191072 MS.gene52475:intron 40.0%
AATCGGGGTAACAGTTCAAA+GGG - chr7.2:61191068-61191087 MS.gene52475:intron 40.0%
ACAAGTGGGAAGAAATTGCA+AGG - chr7.2:61190967-61190986 MS.gene52475:intron 40.0%
ACTCCAGAAGATGAAATTGG+TGG - chr7.2:61191239-61191258 MS.gene52475:intron 40.0%
AGAAATTGCAAGGAGATTGC+CGG - chr7.2:61190977-61190996 MS.gene52475:intron 40.0%
AGTGTGTTCTGCTTCCTTAT+TGG - chr7.2:61190381-61190400 MS.gene52475:CDS 40.0%
ATAAGGAAGCAGAACACACT+AGG + chr7.2:61190381-61190400 None:intergenic 40.0%
ATCGAAGCTCACAAGATAGT+TGG - chr7.2:61190942-61190961 MS.gene52475:intron 40.0%
CCCTGAAATTCCTATAAAGC+AGG - chr7.2:61191334-61191353 MS.gene52475:intron 40.0%
GAAAGCCAAGAAGAAGAATC+GGG - chr7.2:61191052-61191071 MS.gene52475:intron 40.0%
TAAGGAAGCAGAACACACTA+GGG + chr7.2:61190380-61190399 None:intergenic 40.0%
TAGCCACCAATTTCATCTTC+TGG + chr7.2:61191245-61191264 None:intergenic 40.0%
TCTCGGAGCATGATGAATAT+CGG - chr7.2:61191161-61191180 MS.gene52475:intron 40.0%
TGGGTTTGGATCAAATGGAA+TGG - chr7.2:61191304-61191323 MS.gene52475:intron 40.0%
! GATTCTTCTTCTTGGCTTTC+TGG + chr7.2:61191052-61191071 None:intergenic 40.0%
!! ATGATGATGGAATGGCAAGT+GGG - chr7.2:61191285-61191304 MS.gene52475:intron 40.0%
!! GTCAAGTTTGCTAGCCAATA+AGG + chr7.2:61190398-61190417 None:intergenic 40.0%
AAATTGCAAGGAGATTGCCG+GGG - chr7.2:61190979-61190998 MS.gene52475:intron 45.0%
AGAACTCTGTCAAGAACCAC+TGG - chr7.2:61191009-61191028 MS.gene52475:intron 45.0%
CTCGGAGCATGATGAATATC+GGG - chr7.2:61191162-61191181 MS.gene52475:intron 45.0%
GAAATTGCAAGGAGATTGCC+GGG - chr7.2:61190978-61190997 MS.gene52475:intron 45.0%
GAATCGGGGTAACAGTTCAA+AGG - chr7.2:61191067-61191086 MS.gene52475:intron 45.0%
GTGGTTCTTGACAGAGTTCT+CGG + chr7.2:61191009-61191028 None:intergenic 45.0%
GTTACCCCGATTCTTCTTCT+TGG + chr7.2:61191060-61191079 None:intergenic 45.0%
! GCAAGTGGGTTTGGATCAAA+TGG - chr7.2:61191299-61191318 MS.gene52475:intron 45.0%
! TCTTCTTCTTGGCTTTCTGG+CGG + chr7.2:61191049-61191068 None:intergenic 45.0%
!! GATGATGATGGAATGGCAAG+TGG - chr7.2:61191284-61191303 MS.gene52475:intron 45.0%
GAAAGAGTCATGGACCGAAG+AGG - chr7.2:61190905-61190924 MS.gene52475:intron 50.0%
! GATGGAATGGCAAGTGGGTT+TGG - chr7.2:61191290-61191309 MS.gene52475:intron 50.0%
CACACTAGGGCACAGTTGCT+TGG + chr7.2:61190367-61190386 None:intergenic 55.0%
GGCGCTTAGTGGTGTTCCAG+TGG + chr7.2:61191028-61191047 None:intergenic 60.0%
! ACAGAGTTCTCGGTGCGCCC+CGG + chr7.2:61190999-61191018 None:intergenic 65.0%
! GGCTTTCTGGCGGCGCTTAG+TGG + chr7.2:61191039-61191058 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 61190212 61191394 61190212 ID=MS.gene52475
chr7.2 mRNA 61190212 61191394 61190212 ID=MS.gene52475.t1;Parent=MS.gene52475
chr7.2 exon 61191389 61191394 61191389 ID=MS.gene52475.t1.exon1;Parent=MS.gene52475.t1
chr7.2 CDS 61191389 61191394 61191389 ID=cds.MS.gene52475.t1;Parent=MS.gene52475.t1
chr7.2 exon 61190212 61190700 61190212 ID=MS.gene52475.t1.exon2;Parent=MS.gene52475.t1
chr7.2 CDS 61190212 61190700 61190212 ID=cds.MS.gene52475.t1;Parent=MS.gene52475.t1
Gene Sequence

>MS.gene52475

ATGAAGGTATTACATCTATATATAGAGTTACAAATGATAGTGTATGTTAAGCTATTGTAAACCCTATCTTATAGAATCATAACCTCTACTGTAACAAACTTTAGAGTTAGTTACAACTCATTTTAAAACTAGGGTTTCTAATAACTTGTGGACCAAGCAACTGTGCCCTAGTGTGTTCTGCTTCCTTATTGGCTAGCAAACTTGACTCTATCTCTTTAGTATTCTGCTTCAATACATAAATTATATTTTAATAATTATTTTATACATTTAATTTAAGTAAATTGATAAAATTAAGCATTTTTTAAATTGTAATATAAGTTATTAATTTTACGTTTGGATATGTAAAATAAAAAATTTGCAGATTTATTAACCGTTGTGACATTGACAAAATTTTATTTTAGTCTTGCATATTCTATCTAAATATATTATATAGGTGTTTGATTCTGTATCTTGTTTACAACTCTCACGTCCCGATGTTATATACATCACGAGTATTATACATGAATGGATGCAAGAAAACAAAACTTCAGATTGCTTGGAAACAAACAAATGCAATGTCTAATTAGATATATAATTAAAATAGTGTACAAGGATGCAAAACTTTACTTGGGAATAAGCTATTACCAAATTGGTGAATTAATTTAATCATATTTATATAACTAATCCATTTATTAACTTTTTTGAATGATTGAAGAAAGAGTCATGGACCGAAGAGGAAGACAAAATATTGATCGAAGCTCACAAGATAGTTGGTAACAAGTGGGAAGAAATTGCAAGGAGATTGCCGGGGCGCACCGAGAACTCTGTCAAGAACCACTGGAACACCACTAAGCGCCGCCAGAAAGCCAAGAAGAAGAATCGGGGTAACAGTTCAAAGGGAACACTACTTTTGAAATACATAATGGAAGTCACTTCTGCTACGAAAGTTGAAAAGGAGATGATGAATAATTCTCGGAGCATGATGAATATCGGGAATCAGCCAAATTATGAAAGTTCTGAGAGTGATTTTTTCTCTGAAGGTTTGACTACTCCAGAAGATGAAATTGGTGGCTATGTGCCAATGATGTTCAATGATGATGATGGAATGGCAAGTGGGTTTGGATCAAATGGAATGGAGTTTTTCCCTGAAATTCCTATAAAGCAGGAAATTGACCTCATGGAGAAGATATATAGAAACTCTTAA
Protein sequence

>MS.gene52475.t1

MKKESWTEEEDKILIEAHKIVGNKWEEIARRLPGRTENSVKNHWNTTKRRQKAKKKNRGNSSKGTLLLKYIMEVTSATKVEKEMMNNSRSMMNIGNQPNYESSESDFFSEGLTTPEDEIGGYVPMMFNDDDGMASGFGSNGMEFFPEIPIKQEIDLMEKIYRNS