Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52555.t1 | XP_003614339.1 | 97.9 | 335 | 7 | 0 | 1 | 335 | 1 | 335 | 2.40E-180 | 641.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52555.t1 | Q9CAA4 | 52.7 | 334 | 133 | 4 | 1 | 333 | 1 | 310 | 1.3e-84 | 314.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52555.t1 | G7JZB3 | 97.9 | 335 | 7 | 0 | 1 | 335 | 1 | 335 | 1.7e-180 | 641.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene52555.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52555.t1 | MTR_5g048350 | 97.910 | 335 | 7 | 0 | 1 | 335 | 1 | 335 | 0.0 | 684 |
| MS.gene52555.t1 | MTR_5g048350 | 95.224 | 335 | 7 | 1 | 1 | 335 | 1 | 326 | 0.0 | 656 |
| MS.gene52555.t1 | MTR_4g110040 | 47.531 | 324 | 131 | 11 | 35 | 332 | 124 | 434 | 7.66e-79 | 248 |
| MS.gene52555.t1 | MTR_4g110040 | 47.095 | 327 | 134 | 11 | 35 | 335 | 256 | 569 | 2.68e-77 | 247 |
| MS.gene52555.t1 | MTR_7g017320 | 41.935 | 310 | 149 | 7 | 41 | 332 | 47 | 343 | 9.45e-59 | 193 |
| MS.gene52555.t1 | MTR_7g017320 | 47.368 | 171 | 77 | 3 | 41 | 211 | 47 | 204 | 8.29e-41 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52555.t1 | AT1G69010 | 52.695 | 334 | 133 | 4 | 1 | 333 | 1 | 310 | 1.96e-113 | 332 |
| MS.gene52555.t1 | AT5G08130 | 39.535 | 258 | 123 | 4 | 46 | 303 | 158 | 382 | 1.71e-50 | 173 |
| MS.gene52555.t1 | AT5G08130 | 39.147 | 258 | 125 | 4 | 46 | 303 | 158 | 383 | 1.05e-49 | 171 |
| MS.gene52555.t1 | AT5G08130 | 39.535 | 258 | 123 | 4 | 46 | 303 | 282 | 506 | 5.43e-49 | 172 |
| MS.gene52555.t1 | AT5G08130 | 39.535 | 258 | 123 | 4 | 46 | 303 | 278 | 502 | 6.06e-49 | 172 |
| MS.gene52555.t1 | AT5G08130 | 39.147 | 258 | 125 | 4 | 46 | 303 | 278 | 503 | 3.85e-48 | 169 |
| MS.gene52555.t1 | AT5G08130 | 39.147 | 258 | 125 | 4 | 46 | 303 | 278 | 503 | 3.89e-48 | 169 |
| MS.gene52555.t1 | AT5G08130 | 39.147 | 258 | 125 | 4 | 46 | 303 | 278 | 503 | 3.89e-48 | 169 |
| MS.gene52555.t1 | AT5G08130 | 39.147 | 258 | 125 | 4 | 46 | 303 | 282 | 507 | 4.19e-48 | 169 |
| MS.gene52555.t1 | AT5G38860 | 37.700 | 313 | 157 | 8 | 27 | 332 | 16 | 297 | 1.03e-46 | 160 |
| MS.gene52555.t1 | AT5G38860 | 45.963 | 161 | 75 | 2 | 27 | 185 | 16 | 166 | 2.02e-35 | 128 |
Find 83 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCGTGATCTATCATATTA+TGG | 0.225408 | 5.2:+38703556 | None:intergenic |
| GAGGTTGACCGACAAAATTT+TGG | 0.267640 | 5.2:+38703673 | None:intergenic |
| GTATGAGATCCCTCAATATT+TGG | 0.276214 | 5.2:+38704662 | None:intergenic |
| AGGGTACCTTTGTTGGTGTT+AGG | 0.276576 | 5.2:+38706527 | None:intergenic |
| TGTACCTTCCCATCCTCTTC+AGG | 0.299051 | 5.2:-38703418 | MS.gene52555:CDS |
| CACAGCTTCATTCTTATTAG+AGG | 0.312925 | 5.2:-38704617 | MS.gene52555:intron |
| GGGTACCTTTGTTGGTGTTA+GGG | 0.323081 | 5.2:+38706528 | None:intergenic |
| GGCGGAGAGGCAACCTGATT+TGG | 0.323743 | 5.2:-38703508 | MS.gene52555:CDS |
| ATTTCAGTTCAAATTGATCT+TGG | 0.329165 | 5.2:-38701110 | MS.gene52555:CDS |
| CCTGAACCATTCTTTACAAT+TGG | 0.334279 | 5.2:+38703651 | None:intergenic |
| TTTCCGGGAAACGGTGATAC+TGG | 0.349733 | 5.2:+38703627 | None:intergenic |
| ATTCCCTTGCTCGCCAAATC+AGG | 0.384265 | 5.2:+38703495 | None:intergenic |
| TTAAGGAAGGGTACCTTTGT+TGG | 0.391183 | 5.2:+38706520 | None:intergenic |
| TGAGTATGTTCAGTACTTAC+AGG | 0.412725 | 5.2:-38704493 | MS.gene52555:CDS |
| GGTGTTAGGGGCAGAGGAAG+TGG | 0.420329 | 5.2:+38706541 | None:intergenic |
| TGGGAGCCACTAAGCATTGT+TGG | 0.420920 | 5.2:+38703576 | None:intergenic |
| ATCTCTCCAACAATGCTTAG+TGG | 0.434167 | 5.2:-38703582 | MS.gene52555:CDS |
| ATCGTGATCTATCATATTAT+GGG | 0.435013 | 5.2:+38703557 | None:intergenic |
| TTTCAGTTCAAATTGATCTT+GGG | 0.437219 | 5.2:-38701109 | MS.gene52555:CDS |
| CCAATTGTAAAGAATGGTTC+AGG | 0.442919 | 5.2:-38703651 | MS.gene52555:CDS |
| CCTTCAGATTCCAAATATTG+AGG | 0.448909 | 5.2:-38704672 | MS.gene52555:intron |
| CTTCAGATTCCAAATATTGA+GGG | 0.451155 | 5.2:-38704671 | MS.gene52555:intron |
| TCATCAAACTTTCCGGGAAA+CGG | 0.451349 | 5.2:+38703618 | None:intergenic |
| GAACCATTCTTTACAATTGG+AGG | 0.459728 | 5.2:+38703654 | None:intergenic |
| CAGGATGAACTTACAGTTGA+AGG | 0.460627 | 5.2:-38703336 | MS.gene52555:CDS |
| GTACCTTCCCATCCTCTTCA+GGG | 0.461963 | 5.2:-38703417 | MS.gene52555:CDS |
| GGAGATGAGGCTGTGCCACT+TGG | 0.463787 | 5.2:+38701068 | None:intergenic |
| CAACTGTAAGTTCATCCTGC+TGG | 0.473427 | 5.2:+38703340 | None:intergenic |
| ACTCAAGCGCTACAGAGTGC+TGG | 0.475738 | 5.2:-38701155 | MS.gene52555:CDS |
| GGTCCAGTATCACCGTTTCC+CGG | 0.476824 | 5.2:-38703630 | MS.gene52555:CDS |
| CAACCTCCAATTGTAAAGAA+TGG | 0.480239 | 5.2:-38703657 | MS.gene52555:CDS |
| TTTGTTGGTGTTAGGGGCAG+AGG | 0.484917 | 5.2:+38706535 | None:intergenic |
| AAATATGAAGGCTCGTATCA+AGG | 0.490493 | 5.2:-38704459 | MS.gene52555:CDS |
| GCAACCTGATTTGGCGAGCA+AGG | 0.490959 | 5.2:-38703499 | MS.gene52555:CDS |
| TGATAGATCACGATCCGACT+CGG | 0.499986 | 5.2:-38703548 | MS.gene52555:CDS |
| CAAGGGAATTCCTCTGCCTA+TGG | 0.504233 | 5.2:-38703481 | MS.gene52555:CDS |
| TCTTGGGAAGCGTGCAAAGA+AGG | 0.509091 | 5.2:-38701093 | MS.gene52555:CDS |
| TTGAGTTGTTCACTCGTTGT+GGG | 0.512133 | 5.2:+38703363 | None:intergenic |
| ATGAAGGCTCGTATCAAGGC+TGG | 0.517535 | 5.2:-38704455 | MS.gene52555:CDS |
| GGTACCTTTGTTGGTGTTAG+GGG | 0.522122 | 5.2:+38706529 | None:intergenic |
| CTCAAGCGCTACAGAGTGCT+GGG | 0.524190 | 5.2:-38701154 | MS.gene52555:CDS |
| TTGCCTACAACAATGAAAGC+AGG | 0.539094 | 5.2:-38706626 | None:intergenic |
| GATAGATCACGATCCGACTC+GGG | 0.540435 | 5.2:-38703547 | MS.gene52555:CDS |
| TACCTCAGTGCGATCAAAAG+AGG | 0.540969 | 5.2:-38704642 | MS.gene52555:CDS |
| AGATGGGAAAGCTACTGATA+AGG | 0.542435 | 5.2:-38705432 | MS.gene52555:CDS |
| AAACAGTTCCCTGAAGAGGA+TGG | 0.542486 | 5.2:+38703409 | None:intergenic |
| GTTGAGTTGTTCACTCGTTG+TGG | 0.544799 | 5.2:+38703362 | None:intergenic |
| AACCGTCGAAGTTGATGCCA+TGG | 0.544835 | 5.2:-38704425 | MS.gene52555:CDS |
| TCTGTTCCTGTCAGAAGTGA+TGG | 0.550958 | 5.2:-38703441 | MS.gene52555:CDS |
| AATGATTGATTGCTACAAAG+AGG | 0.557171 | 5.2:+38699787 | None:intergenic |
| ACCCATGGCATCAACTTCGA+CGG | 0.559522 | 5.2:+38704423 | None:intergenic |
| TCTGTATACCTTGGGAGATG+AGG | 0.560824 | 5.2:+38701055 | None:intergenic |
| AACAGTTCCCTGAAGAGGAT+GGG | 0.568210 | 5.2:+38703410 | None:intergenic |
| ATCTCGAGTGTATACTCCCA+AGG | 0.571871 | 5.2:-38703300 | MS.gene52555:CDS |
| ATGATTGATTGCTACAAAGA+GGG | 0.583430 | 5.2:+38699788 | None:intergenic |
| ACATTCAGTTGATTGTGCAT+CGG | 0.585244 | 5.2:+38703386 | None:intergenic |
| ATGTTTGCATGCATAGCCAT+AGG | 0.589737 | 5.2:+38703465 | None:intergenic |
| GATCCGACTCGGGATATTGT+CGG | 0.590573 | 5.2:-38703537 | MS.gene52555:CDS |
| CAACCTGATTTGGCGAGCAA+GGG | 0.591521 | 5.2:-38703498 | MS.gene52555:CDS |
| GAAGAAGCAAGATAAATGAG+AGG | 0.595996 | 5.2:-38705367 | MS.gene52555:intron |
| TCGGAAACAGTTCCCTGAAG+AGG | 0.597102 | 5.2:+38703405 | None:intergenic |
| TGCCTACAACAATGAAAGCA+GGG | 0.600473 | 5.2:-38706625 | None:intergenic |
| GAGTGAACAACTCAACCAGC+AGG | 0.605106 | 5.2:-38703355 | MS.gene52555:CDS |
| GGTACACCATCACTTCTGAC+AGG | 0.608676 | 5.2:+38703435 | None:intergenic |
| GCATGCATAGCCATAGGCAG+AGG | 0.609045 | 5.2:+38703471 | None:intergenic |
| ACCTCAGTGCGATCAAAAGA+GGG | 0.614955 | 5.2:-38704641 | MS.gene52555:CDS |
| TCGGGATATTGTCGGCAAGA+CGG | 0.618174 | 5.2:-38703529 | MS.gene52555:CDS |
| AACATTCTGTAACGGAGCAG+CGG | 0.620536 | 5.2:-38705391 | MS.gene52555:CDS |
| TGGCACAGCCTCATCTCCCA+AGG | 0.623367 | 5.2:-38701063 | MS.gene52555:intron |
| ACCGTCGAAGTTGATGCCAT+GGG | 0.624180 | 5.2:-38704424 | MS.gene52555:intron |
| AAGATCGAAACATTCTGTAA+CGG | 0.625212 | 5.2:-38705399 | MS.gene52555:CDS |
| ATGTTCAGTACTTACAGGAG+AGG | 0.638295 | 5.2:-38704488 | MS.gene52555:CDS |
| TGCTGGGTTAGATCTGTCAA+AGG | 0.641347 | 5.2:-38701138 | MS.gene52555:CDS |
| GTTCCCTGAAGAGGATGGGA+AGG | 0.644437 | 5.2:+38703414 | None:intergenic |
| GATGAACTTACAGTTGAAGG+AGG | 0.651861 | 5.2:-38703333 | MS.gene52555:CDS |
| TGTTCAGTACTTACAGGAGA+GGG | 0.653015 | 5.2:-38704487 | MS.gene52555:CDS |
| TCTCGAGTGTATACTCCCAA+GGG | 0.653032 | 5.2:-38703299 | MS.gene52555:intron |
| GGATATTGTCGGCAAGACGG+CGG | 0.664149 | 5.2:-38703526 | MS.gene52555:CDS |
| ATGAACTTACAGTTGAAGGA+GGG | 0.671653 | 5.2:-38703332 | MS.gene52555:CDS |
| TTGCCGACAATATCCCGAGT+CGG | 0.672673 | 5.2:+38703534 | None:intergenic |
| TTGTCGGCAAGACGGCGGAG+AGG | 0.684251 | 5.2:-38703521 | MS.gene52555:CDS |
| TCTGCCCCTAACACCAACAA+AGG | 0.684939 | 5.2:-38706533 | MS.gene52555:intron |
| CGTGCAAAGAAGGATCCAAG+TGG | 0.732676 | 5.2:-38701083 | MS.gene52555:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 38699714 | 38706636 | 38699714 | ID=MS.gene52555 |
| chr5.2 | mRNA | 38699714 | 38706636 | 38699714 | ID=MS.gene52555.t1;Parent=MS.gene52555 |
| chr5.2 | exon | 38706534 | 38706636 | 38706534 | ID=MS.gene52555.t1.exon1;Parent=MS.gene52555.t1 |
| chr5.2 | CDS | 38706534 | 38706636 | 38706534 | ID=cds.MS.gene52555.t1;Parent=MS.gene52555.t1 |
| chr5.2 | exon | 38705368 | 38705452 | 38705368 | ID=MS.gene52555.t1.exon2;Parent=MS.gene52555.t1 |
| chr5.2 | CDS | 38705368 | 38705452 | 38705368 | ID=cds.MS.gene52555.t1;Parent=MS.gene52555.t1 |
| chr5.2 | exon | 38704618 | 38704687 | 38704618 | ID=MS.gene52555.t1.exon3;Parent=MS.gene52555.t1 |
| chr5.2 | CDS | 38704618 | 38704687 | 38704618 | ID=cds.MS.gene52555.t1;Parent=MS.gene52555.t1 |
| chr5.2 | exon | 38704425 | 38704520 | 38704425 | ID=MS.gene52555.t1.exon4;Parent=MS.gene52555.t1 |
| chr5.2 | CDS | 38704425 | 38704520 | 38704425 | ID=cds.MS.gene52555.t1;Parent=MS.gene52555.t1 |
| chr5.2 | exon | 38703300 | 38703715 | 38703300 | ID=MS.gene52555.t1.exon5;Parent=MS.gene52555.t1 |
| chr5.2 | CDS | 38703300 | 38703715 | 38703300 | ID=cds.MS.gene52555.t1;Parent=MS.gene52555.t1 |
| chr5.2 | exon | 38701064 | 38701193 | 38701064 | ID=MS.gene52555.t1.exon6;Parent=MS.gene52555.t1 |
| chr5.2 | CDS | 38701064 | 38701193 | 38701064 | ID=cds.MS.gene52555.t1;Parent=MS.gene52555.t1 |
| chr5.2 | exon | 38699714 | 38699821 | 38699714 | ID=MS.gene52555.t1.exon7;Parent=MS.gene52555.t1 |
| chr5.2 | CDS | 38699714 | 38699821 | 38699714 | ID=cds.MS.gene52555.t1;Parent=MS.gene52555.t1 |
| Gene Sequence |
| Protein sequence |
