AlfalfaGEDB | MS.gene52573

M. sativa cultivar XinJiangDaYe / MS.gene52573

KEGG: K18835
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52573.t1	AES80392.2	86.8	106	14	0	1	106	408	513	2.00E-43	184.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52573.t1	O65590	56.8	88	38	0	19	106	305	392	4.9e-19	95.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52573.t1	G7KRI3	86.8	106	14	0	1	106	408	513	1.4e-43	184.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene52573.t1	TF	WRKY

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52573.t1	MTR_7g080020	86.792	106	14	0	1	106	408	513	6.74e-60	195
MS.gene52573.t1	MTR_7g080000	85.849	106	9	2	1	106	353	452	2.83e-54	179
MS.gene52573.t1	MTR_3g009470	54.639	97	43	1	10	106	420	515	3.04e-26	101
MS.gene52573.t1	MTR_3g009470	54.639	97	43	1	10	106	420	515	3.65e-26	101
MS.gene52573.t1	MTR_3g056100	51.064	94	41	3	14	106	346	435	2.76e-21	87.4
MS.gene52573.t1	MTR_3g056100	51.064	94	41	3	14	106	333	422	3.03e-21	87.4
MS.gene52573.t1	MTR_3g056100	51.064	94	41	3	14	106	189	278	3.17e-21	87.0
MS.gene52573.t1	MTR_3g056100	51.064	94	41	3	14	106	202	291	3.52e-21	87.0
MS.gene52573.t1	MTR_5g094430	49.425	87	44	0	20	106	186	272	2.23e-20	84.7
MS.gene52573.t1	MTR_5g094430	49.425	87	44	0	20	106	164	250	2.33e-20	84.3
MS.gene52573.t1	MTR_5g094430	49.425	87	44	0	20	106	298	384	2.44e-20	84.7
MS.gene52573.t1	MTR_3g031220	61.667	60	23	0	47	106	326	385	1.10e-19	82.4
MS.gene52573.t1	MTR_3g031220	61.667	60	23	0	47	106	326	385	1.61e-19	82.4
MS.gene52573.t1	MTR_5g074400	58.462	65	25	1	44	106	376	440	3.63e-19	81.3
MS.gene52573.t1	MTR_4g013260	46.018	113	49	4	2	106	248	356	4.79e-19	80.9
MS.gene52573.t1	MTR_4g082580	47.674	86	36	2	30	106	213	298	7.35e-19	80.1
MS.gene52573.t1	MTR_7g011820	50.725	69	32	1	40	106	362	430	9.75e-18	77.4
MS.gene52573.t1	MTR_7g100510	54.688	64	29	0	43	106	318	381	6.35e-17	74.7
MS.gene52573.t1	MTR_7g071120	72.500	40	11	0	67	106	305	344	8.06e-17	74.7
MS.gene52573.t1	MTR_5g042920	57.627	59	24	1	49	106	282	340	1.63e-16	73.6
MS.gene52573.t1	MTR_8g007270	65.854	41	14	0	66	106	134	174	3.45e-14	65.9
MS.gene52573.t1	MTR_1g077760	65.000	40	14	0	67	106	95	134	2.04e-13	62.0
MS.gene52573.t1	MTR_4g130900	37.234	94	56	1	13	106	289	379	5.29e-13	63.9
MS.gene52573.t1	MTR_3g095080	63.158	38	14	0	69	106	151	188	2.75e-12	60.1
MS.gene52573.t1	MTR_3g095080	63.158	38	14	0	69	106	127	164	3.87e-12	60.1
MS.gene52573.t1	MTR_3g095080	63.158	38	14	0	69	106	151	188	4.09e-12	60.5
MS.gene52573.t1	MTR_1g077780	57.500	40	17	0	67	106	43	82	3.13e-11	56.6
MS.gene52573.t1	MTR_3g104750	51.220	41	20	0	66	106	84	124	4.05e-11	56.6
MS.gene52573.t1	MTR_5g097490	50.000	58	29	0	49	106	215	272	8.84e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52573.t1	AT4G26440	58.140	86	36	0	21	106	307	392	1.38e-25	99.8
MS.gene52573.t1	AT5G56270	72.881	59	16	0	48	106	449	507	2.85e-24	95.9
MS.gene52573.t1	AT5G07100	48.864	88	28	3	19	106	91	161	7.29e-20	80.5
MS.gene52573.t1	AT5G07100	48.864	88	28	3	19	106	91	161	7.29e-20	80.5
MS.gene52573.t1	AT5G07100	48.864	88	28	3	19	106	91	161	7.29e-20	80.5
MS.gene52573.t1	AT5G07100	48.864	88	28	3	19	106	91	161	7.29e-20	80.5
MS.gene52573.t1	AT5G07100	48.864	88	28	3	19	106	184	254	2.07e-19	80.9
MS.gene52573.t1	AT4G26640	53.750	80	34	2	29	106	147	225	7.59e-19	80.1
MS.gene52573.t1	AT4G26640	53.750	80	34	2	29	106	147	225	7.59e-19	80.1
MS.gene52573.t1	AT4G26640	53.750	80	34	2	29	106	147	225	7.59e-19	80.1
MS.gene52573.t1	AT4G26640	53.750	80	34	2	29	106	147	225	7.59e-19	80.1
MS.gene52573.t1	AT4G26640	53.750	80	34	2	29	106	323	401	8.65e-19	80.5
MS.gene52573.t1	AT4G26640	60.317	63	24	1	44	106	300	361	9.55e-19	80.1
MS.gene52573.t1	AT4G26640	53.750	80	34	2	29	106	251	329	9.67e-19	80.1
MS.gene52573.t1	AT2G38470	77.500	40	9	0	67	106	343	382	1.29e-18	79.7
MS.gene52573.t1	AT2G03340	46.939	98	29	3	30	106	340	435	2.94e-17	75.9
MS.gene52573.t1	AT1G13960	72.500	40	11	0	67	106	390	429	2.63e-16	73.2
MS.gene52573.t1	AT1G13960	72.500	40	11	0	67	106	237	276	2.96e-16	72.8
MS.gene52573.t1	AT4G12020	45.556	90	43	2	18	106	577	661	3.92e-16	72.8
MS.gene52573.t1	AT4G12020	45.556	90	43	2	18	106	577	661	4.04e-16	72.8
MS.gene52573.t1	AT4G12020	45.556	90	43	2	18	106	577	661	4.04e-16	72.8
MS.gene52573.t1	AT4G12020	45.556	90	43	2	18	106	577	661	4.29e-16	72.8
MS.gene52573.t1	AT4G12020	45.556	90	43	2	18	106	577	661	4.33e-16	72.8
MS.gene52573.t1	AT4G12020	45.556	90	43	2	18	106	577	661	4.38e-16	72.8
MS.gene52573.t1	AT4G12020	45.556	90	43	2	18	106	577	661	4.46e-16	72.8
MS.gene52573.t1	AT4G12020	45.556	90	43	2	18	106	577	661	4.46e-16	72.8
MS.gene52573.t1	AT4G12020	45.556	90	43	2	18	106	577	661	4.46e-16	72.8
MS.gene52573.t1	AT4G12020	45.556	90	43	2	18	106	510	594	4.50e-16	72.8
MS.gene52573.t1	AT4G12020	45.556	90	43	2	18	106	577	661	4.59e-16	72.8
MS.gene52573.t1	AT3G01080	68.293	41	13	0	66	106	324	364	4.78e-16	72.4
MS.gene52573.t1	AT3G01080	70.000	40	12	0	67	106	287	326	5.15e-16	72.4
MS.gene52573.t1	AT2G30250	72.500	40	11	0	67	106	309	348	2.84e-15	70.1
MS.gene52573.t1	AT2G44745	65.854	41	14	0	66	106	125	165	2.07e-14	66.2
MS.gene52573.t1	AT4G39410	54.000	50	23	0	57	106	194	243	2.35e-13	64.3
MS.gene52573.t1	AT2G04880	37.037	81	51	0	26	106	247	327	3.53e-13	64.3
MS.gene52573.t1	AT2G04880	37.037	81	51	0	26	106	223	303	3.87e-13	64.3
MS.gene52573.t1	AT1G55600	62.000	50	18	1	58	106	278	327	5.63e-13	63.5
MS.gene52573.t1	AT1G69310	46.377	69	32	1	43	106	99	167	5.88e-12	60.5
MS.gene52573.t1	AT1G69310	46.377	69	32	1	43	106	99	167	6.85e-12	60.5
MS.gene52573.t1	AT1G69310	46.377	69	32	1	43	106	99	167	6.85e-12	60.5
MS.gene52573.t1	AT1G69310	46.377	69	32	1	43	106	99	167	6.85e-12	60.5
MS.gene52573.t1	AT5G41570	60.976	41	16	0	66	106	78	118	1.91e-11	57.8
MS.gene52573.t1	AT3G01970	67.647	34	11	0	73	106	52	85	3.45e-11	56.6
MS.gene52573.t1	AT1G64000	58.537	41	17	0	66	106	94	134	8.59e-11	56.2
MS.gene52573.t1	AT4G30935	43.939	66	21	1	41	106	302	351	9.92e-11	57.4

Find 30 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGATTCTGAGTCGGAATCTT+CGG	0.317084	7.4:-26145895	None:intergenic
GGTTATCGCTGGCGAAAGTA+TGG	0.322826	7.4:+26146125	MS.gene52573:CDS
CAATATGACTACCTTGGGTT+TGG	0.333947	7.4:-26146170	None:intergenic
CGAGGCACCATTGTTTCAAC+CGG	0.375364	7.4:-26146038	None:intergenic
AAAAGCAATATGACTACCTT+GGG	0.438859	7.4:-26146175	None:intergenic
TTTGAATCAGACGACTCTCC+GGG	0.463891	7.4:+26145795	MS.gene52573:CDS
GTTTGAATCAGACGACTCTC+CGG	0.479499	7.4:+26145794	MS.gene52573:CDS
TCCGCACTTGCTAATCATGA+TGG	0.511029	7.4:+26145825	MS.gene52573:CDS
CATCGATGTCACTCTCACTT+TGG	0.518656	7.4:-26146091	None:intergenic
CCGACTCAGAATCGAAAAGA+AGG	0.525017	7.4:+26145904	MS.gene52573:CDS
TGAAACAATGGTGCCTCGGA+GGG	0.551047	7.4:+26146043	MS.gene52573:CDS
GCAAGTGCGGAAGAATGCCC+CGG	0.553258	7.4:-26145813	None:intergenic
ATGGACAGAAGGTTGTCAAA+GGG	0.557821	7.4:+26146144	MS.gene52573:CDS
TATGGACAGAAGGTTGTCAA+AGG	0.558376	7.4:+26146143	MS.gene52573:CDS
ATGAAGAGAGCTAGATCTCA+AGG	0.562206	7.4:+26145768	MS.gene52573:CDS
AGGTGATAAGACTGCTTGAG+TGG	0.567812	7.4:-26145862	None:intergenic
GAGCTACCCGGTTGAAACAA+TGG	0.579050	7.4:+26146031	MS.gene52573:CDS
CGGTTGAAACAATGGTGCCT+CGG	0.589928	7.4:+26146039	MS.gene52573:CDS
TACTTGATGATGGTTATCGC+TGG	0.598356	7.4:+26146114	MS.gene52573:CDS
CTGGCGAAAGTATGGACAGA+AGG	0.605120	7.4:+26146133	MS.gene52573:CDS
GACATCGATGTACTTGATGA+TGG	0.605527	7.4:+26146104	MS.gene52573:CDS
CAGGAAGAAGGAGAGCTACC+CGG	0.610711	7.4:+26146019	MS.gene52573:intron
TTGAAACAATGGTGCCTCGG+AGG	0.618982	7.4:+26146042	MS.gene52573:CDS
ACCATCATGATTAGCAAGTG+CGG	0.620117	7.4:-26145826	None:intergenic
AATCATGATGGTGATACAGA+TGG	0.629440	7.4:+26145837	MS.gene52573:CDS
TCAAAGGGAATCCAAACCCA+AGG	0.645747	7.4:+26146159	MS.gene52573:CDS
GAGGCACCATTGTTTCAACC+GGG	0.652598	7.4:-26146037	None:intergenic
GGCGCACGAATTGCCCTCCG+AGG	0.656268	7.4:-26146056	None:intergenic
TCACTTTGGACTATCACTCG+TGG	0.680114	7.4:-26146077	None:intergenic
TTGAATCAGACGACTCTCCG+GGG	0.693866	7.4:+26145796	MS.gene52573:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTGGTTAAAATTTTTGTTGC+AGG	+	chr7.4:26146000-26146019	MS.gene52573:intron	25.0%
	AGACAATCTATATACGTTGT+TGG	+	chr7.4:26145981-26146000	MS.gene52573:intron	30.0%
!	AAATTTTTGTTGCAGGAAGA+AGG	+	chr7.4:26146007-26146026	MS.gene52573:intron	30.0%
	ATCTGATACTGTCTTCATGT+AGG	+	chr7.4:26145938-26145957	MS.gene52573:intron	35.0%
!	AATCATGATGGTGATACAGA+TGG	+	chr7.4:26145837-26145856	MS.gene52573:CDS	35.0%
!	GAATCTTCGGAATTGTTTTC+AGG	-	chr7.4:26145885-26145904	None:intergenic	35.0%
	ACCATCATGATTAGCAAGTG+CGG	-	chr7.4:26145829-26145848	None:intergenic	40.0%
	ATGAAGAGAGCTAGATCTCA+AGG	+	chr7.4:26145768-26145787	MS.gene52573:CDS	40.0%
	ATGGACAGAAGGTTGTCAAA+GGG	+	chr7.4:26146144-26146163	MS.gene52573:CDS	40.0%
	GACATCGATGTACTTGATGA+TGG	+	chr7.4:26146104-26146123	MS.gene52573:CDS	40.0%
	TACTGTCTTCATGTAGGCAT+TGG	+	chr7.4:26145944-26145963	MS.gene52573:intron	40.0%
	TATGGACAGAAGGTTGTCAA+AGG	+	chr7.4:26146143-26146162	MS.gene52573:CDS	40.0%
!	CCTTCTTTTCGATTCTGAGT+CGG	-	chr7.4:26145907-26145926	None:intergenic	40.0%
!!	TACTTGATGATGGTTATCGC+TGG	+	chr7.4:26146114-26146133	MS.gene52573:CDS	40.0%
	AGGTGATAAGACTGCTTGAG+TGG	-	chr7.4:26145865-26145884	None:intergenic	45.0%
	CATCGATGTCACTCTCACTT+TGG	-	chr7.4:26146094-26146113	None:intergenic	45.0%
	CCGACTCAGAATCGAAAAGA+AGG	+	chr7.4:26145904-26145923	MS.gene52573:CDS	45.0%
	CGATTCTGAGTCGGAATCTT+CGG	-	chr7.4:26145898-26145917	None:intergenic	45.0%
	GTTTGAATCAGACGACTCTC+CGG	+	chr7.4:26145794-26145813	MS.gene52573:CDS	45.0%
	TCAAAGGGAATCCAAACCCA+AGG	+	chr7.4:26146159-26146178	MS.gene52573:CDS	45.0%
	TCACTTTGGACTATCACTCG+TGG	-	chr7.4:26146080-26146099	None:intergenic	45.0%
	TTTGAATCAGACGACTCTCC+GGG	+	chr7.4:26145795-26145814	MS.gene52573:CDS	45.0%
!	TCCGCACTTGCTAATCATGA+TGG	+	chr7.4:26145825-26145844	MS.gene52573:CDS	45.0%
	CGGTTGAAACAATGGTGCCT+CGG	+	chr7.4:26146039-26146058	MS.gene52573:CDS	50.0%
	GAGCTACCCGGTTGAAACAA+TGG	+	chr7.4:26146031-26146050	MS.gene52573:CDS	50.0%
	GGTTATCGCTGGCGAAAGTA+TGG	+	chr7.4:26146125-26146144	MS.gene52573:CDS	50.0%
	TGAAACAATGGTGCCTCGGA+GGG	+	chr7.4:26146043-26146062	MS.gene52573:CDS	50.0%
	TTGAAACAATGGTGCCTCGG+AGG	+	chr7.4:26146042-26146061	MS.gene52573:CDS	50.0%
	TTGAATCAGACGACTCTCCG+GGG	+	chr7.4:26145796-26145815	MS.gene52573:CDS	50.0%
!	CGAGGCACCATTGTTTCAAC+CGG	-	chr7.4:26146041-26146060	None:intergenic	50.0%
!	CTGGCGAAAGTATGGACAGA+AGG	+	chr7.4:26146133-26146152	MS.gene52573:CDS	50.0%
!	GAGGCACCATTGTTTCAACC+GGG	-	chr7.4:26146040-26146059	None:intergenic	50.0%
	CAGGAAGAAGGAGAGCTACC+CGG	+	chr7.4:26146019-26146038	MS.gene52573:intron	55.0%
	GCAAGTGCGGAAGAATGCCC+CGG	-	chr7.4:26145816-26145835	None:intergenic	60.0%
	GGCGCACGAATTGCCCTCCG+AGG	-	chr7.4:26146059-26146078	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	26145768	26146184	26145768	ID=MS.gene52573
chr7.4	mRNA	26145768	26146184	26145768	ID=MS.gene52573.t1;Parent=MS.gene52573
chr7.4	exon	26145768	26145925	26145768	ID=MS.gene52573.t1.exon1;Parent=MS.gene52573.t1
chr7.4	CDS	26145768	26145925	26145768	ID=cds.MS.gene52573.t1;Parent=MS.gene52573.t1
chr7.4	exon	26146022	26146184	26146022	ID=MS.gene52573.t1.exon2;Parent=MS.gene52573.t1
chr7.4	CDS	26146022	26146184	26146022	ID=cds.MS.gene52573.t1;Parent=MS.gene52573.t1

Gene Sequence

>MS.gene52573

ATGAAGAGAGCTAGATCTCAAGGTAAGTTTGAATCAGACGACTCTCCGGGGCATTCTTCCGCACTTGCTAATCATGATGGTGATACAGATGGAGCCACTCAAGCAGTCTTATCACCTGAAAACAATTCCGAAGATTCCGACTCAGAATCGAAAAGAAGGTATTGTATTTTATCTGATACTGTCTTCATGTAGGCATTGGCAATACAAATAGAAAGACAATCTATATACGTTGTTGGTTAAAATTTTTGTTGCAGGAAGAAGGAGAGCTACCCGGTTGAAACAATGGTGCCTCGGAGGGCAATTCGTGCGCCACGAGTGATAGTCCAAAGTGAGAGTGACATCGATGTACTTGATGATGGTTATCGCTGGCGAAAGTATGGACAGAAGGTTGTCAAAGGGAATCCAAACCCAAGGTAG

Protein sequence

>MS.gene52573.t1

MKRARSQGKFESDDSPGHSSALANHDGDTDGATQAVLSPENNSEDSDSESKRRKKESYPVETMVPRRAIRAPRVIVQSESDIDVLDDGYRWRKYGQKVVKGNPNPR