Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52784.t1 | XP_024632959.1 | 51.5 | 330 | 83 | 7 | 1 | 253 | 1 | 330 | 1.50E-65 | 259.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52784.t1 | Q9SSW2 | 40.2 | 97 | 39 | 4 | 45 | 137 | 106 | 187 | 2.9e-09 | 63.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52784.t1 | A0A396JGH2 | 51.5 | 330 | 83 | 7 | 1 | 253 | 1 | 330 | 1.1e-65 | 259.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene52784.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52784.t1 | MTR_6g081160 | 34.266 | 286 | 108 | 8 | 27 | 253 | 8 | 272 | 1.49e-31 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52784.t1 | AT3G19580 | 40.206 | 97 | 39 | 4 | 45 | 137 | 106 | 187 | 1.38e-13 | 69.3 |
| MS.gene52784.t1 | AT3G19580 | 40.206 | 97 | 39 | 4 | 45 | 137 | 106 | 187 | 1.38e-13 | 69.3 |
| MS.gene52784.t1 | AT1G49900 | 39.130 | 92 | 38 | 2 | 46 | 137 | 194 | 267 | 1.21e-11 | 64.7 |
| MS.gene52784.t1 | AT5G67450 | 39.175 | 97 | 48 | 2 | 45 | 137 | 97 | 186 | 3.69e-11 | 62.0 |
Find 36 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAATTTCTCTTTACCAAAA+TGG | 0.235457 | 3.1:-91137700 | MS.gene52784:CDS |
| AAGCATGAGGTTATTATTAC+AGG | 0.279433 | 3.1:-91138118 | MS.gene52784:CDS |
| TCTAACTTTATCTTCTTAAG+AGG | 0.301299 | 3.1:+91138019 | None:intergenic |
| TCCGACTCTTCATAAGTATT+CGG | 0.323788 | 3.1:+91137971 | None:intergenic |
| CCTCTTGTCAGACTCTTCAT+AGG | 0.349643 | 3.1:+91138186 | None:intergenic |
| CATAAAGAAGATCAAGCTTA+TGG | 0.366706 | 3.1:-91137722 | MS.gene52784:CDS |
| TTACGAATAACATGATTTGC+TGG | 0.441171 | 3.1:+91137785 | None:intergenic |
| GAATGAGCCATATGCTATTG+AGG | 0.477019 | 3.1:-91137486 | MS.gene52784:CDS |
| AGAAGAGGTAGAAAGTGTAT+TGG | 0.483023 | 3.1:-91137665 | MS.gene52784:CDS |
| GCTTAGTCCTCAATAGCATA+TGG | 0.486464 | 3.1:+91137479 | None:intergenic |
| TTGGTGAGTCATCATCAAAT+TGG | 0.494071 | 3.1:-91137586 | MS.gene52784:CDS |
| CTAACTTTATCTTCTTAAGA+GGG | 0.512429 | 3.1:+91138020 | None:intergenic |
| TGAGATCTCCGGTGAGGAAG+GGG | 0.514342 | 3.1:+91137994 | None:intergenic |
| ACTGATGATCAGCTAAAGAA+AGG | 0.524124 | 3.1:-91137527 | MS.gene52784:CDS |
| GTGAGATCTCCGGTGAGGAA+GGG | 0.530993 | 3.1:+91137993 | None:intergenic |
| TAAGTATTCGGTGAGATCTC+CGG | 0.541580 | 3.1:+91137983 | None:intergenic |
| TTTAGCAGCGGACAAGCACT+TGG | 0.544373 | 3.1:-91138061 | MS.gene52784:CDS |
| CTTATGAAGAGTCGGAAAAG+AGG | 0.546986 | 3.1:-91137964 | MS.gene52784:CDS |
| GAAAAGCATTGGGTGGTCAC+CGG | 0.556634 | 3.1:-91137841 | MS.gene52784:CDS |
| TCTTCTTTATGATGTTCAGT+AGG | 0.559242 | 3.1:+91137734 | None:intergenic |
| GGTGAGATCTCCGGTGAGGA+AGG | 0.559786 | 3.1:+91137992 | None:intergenic |
| GTTAGAAACCCCTTCCTCAC+CGG | 0.572034 | 3.1:-91138002 | MS.gene52784:CDS |
| ACCGAATACTTATGAAGAGT+CGG | 0.572679 | 3.1:-91137972 | MS.gene52784:CDS |
| TCTTAATAGAGTGAGATCTC+CGG | 0.581386 | 3.1:+91137822 | None:intergenic |
| GAGTCATCATCAAATTGGAA+TGG | 0.587360 | 3.1:-91137581 | MS.gene52784:CDS |
| TGGCAAAAGAAAGATAGAAG+AGG | 0.598017 | 3.1:-91137680 | MS.gene52784:CDS |
| AGCAGCGGACAAGCACTTGG+TGG | 0.609210 | 3.1:-91138058 | MS.gene52784:CDS |
| TCTTGTTCACTATCAAGTGA+CGG | 0.609728 | 3.1:-91138151 | MS.gene52784:CDS |
| CCTATGAAGAGTCTGACAAG+AGG | 0.641227 | 3.1:-91138186 | MS.gene52784:CDS |
| CGAAGATGATGTCAAGCATG+AGG | 0.647694 | 3.1:-91137909 | MS.gene52784:CDS |
| CGGAGATGATGCAAAGCATG+AGG | 0.655546 | 3.1:-91138131 | MS.gene52784:CDS |
| GTGTATTGGTAGTGTTGAAG+CGG | 0.655661 | 3.1:-91137651 | MS.gene52784:CDS |
| AAAAGTACTTATCTGAACGA+AGG | 0.659168 | 3.1:+91137612 | None:intergenic |
| TGGAATGGCAAGAATGAGCA+TGG | 0.683517 | 3.1:-91137566 | MS.gene52784:CDS |
| ATTCGGTGAGATCTCCGGTG+AGG | 0.731513 | 3.1:+91137988 | None:intergenic |
| AATGGCAAGAATGAGCATGG+TGG | 0.807756 | 3.1:-91137563 | MS.gene52784:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGCAGAAAAACTTTTAAAAA+TGG | - | chr3.1:91137838-91137857 | MS.gene52784:CDS | 20.0% |
| !!! | TTTAAAAATGGAAAAGCATT+GGG | - | chr3.1:91137850-91137869 | MS.gene52784:CDS | 20.0% |
| !!! | TTTTAAAAATGGAAAAGCAT+TGG | - | chr3.1:91137849-91137868 | MS.gene52784:CDS | 20.0% |
| ! | CTAACTTTATCTTCTTAAGA+GGG | + | chr3.1:91137684-91137703 | None:intergenic | 25.0% |
| ! | GTAATTTCTCTTTACCAAAA+TGG | - | chr3.1:91138001-91138020 | MS.gene52784:CDS | 25.0% |
| ! | TCTAACTTTATCTTCTTAAG+AGG | + | chr3.1:91137685-91137704 | None:intergenic | 25.0% |
| ! | TGTGAAAAAAAATTTAGCAG+CGG | - | chr3.1:91137628-91137647 | MS.gene52784:CDS | 25.0% |
| AAGCATGAGGTTATTATTAC+AGG | - | chr3.1:91137583-91137602 | MS.gene52784:CDS | 30.0% | |
| CATAAAGAAGATCAAGCTTA+TGG | - | chr3.1:91137979-91137998 | MS.gene52784:CDS | 30.0% | |
| TCTTCTTTATGATGTTCAGT+AGG | + | chr3.1:91137970-91137989 | None:intergenic | 30.0% | |
| ! | GTTTTCTCATAATCATGCTT+AGG | + | chr3.1:91137496-91137515 | None:intergenic | 30.0% |
| ! | TCAGATAAGTACTTTTGTGT+TGG | - | chr3.1:91138096-91138115 | MS.gene52784:CDS | 30.0% |
| ! | TTACGAATAACATGATTTGC+TGG | + | chr3.1:91137919-91137938 | None:intergenic | 30.0% |
| ! | TTTTTCTGCAAATATTGCAC+TGG | + | chr3.1:91137828-91137847 | None:intergenic | 30.0% |
| !! | AAAAGTACTTATCTGAACGA+AGG | + | chr3.1:91138092-91138111 | None:intergenic | 30.0% |
| !! | CTATCTTTCTTTTGCCATTT+TGG | + | chr3.1:91138018-91138037 | None:intergenic | 30.0% |
| ACCGAATACTTATGAAGAGT+CGG | - | chr3.1:91137729-91137748 | MS.gene52784:CDS | 35.0% | |
| ACTGATGATCAGCTAAAGAA+AGG | - | chr3.1:91138174-91138193 | MS.gene52784:CDS | 35.0% | |
| GAGTCATCATCAAATTGGAA+TGG | - | chr3.1:91138120-91138139 | MS.gene52784:CDS | 35.0% | |
| TCCGACTCTTCATAAGTATT+CGG | + | chr3.1:91137733-91137752 | None:intergenic | 35.0% | |
| TCTTAATAGAGTGAGATCTC+CGG | + | chr3.1:91137882-91137901 | None:intergenic | 35.0% | |
| TCTTGTTCACTATCAAGTGA+CGG | - | chr3.1:91137550-91137569 | MS.gene52784:CDS | 35.0% | |
| TGGCAAAAGAAAGATAGAAG+AGG | - | chr3.1:91138021-91138040 | MS.gene52784:CDS | 35.0% | |
| TTGGTGAGTCATCATCAAAT+TGG | - | chr3.1:91138115-91138134 | MS.gene52784:CDS | 35.0% | |
| ! | AGAAGAGGTAGAAAGTGTAT+TGG | - | chr3.1:91138036-91138055 | MS.gene52784:CDS | 35.0% |
| !! | AAAAATGGAAAAGCATTGGG+TGG | - | chr3.1:91137853-91137872 | MS.gene52784:CDS | 35.0% |
| CTTATGAAGAGTCGGAAAAG+AGG | - | chr3.1:91137737-91137756 | MS.gene52784:CDS | 40.0% | |
| GAATGAGCCATATGCTATTG+AGG | - | chr3.1:91138215-91138234 | MS.gene52784:CDS | 40.0% | |
| ! | TAAGTATTCGGTGAGATCTC+CGG | + | chr3.1:91137721-91137740 | None:intergenic | 40.0% |
| !! | GTGTATTGGTAGTGTTGAAG+CGG | - | chr3.1:91138050-91138069 | MS.gene52784:CDS | 40.0% |
| AATGGCAAGAATGAGCATGG+TGG | - | chr3.1:91138138-91138157 | MS.gene52784:CDS | 45.0% | |
| CCTATGAAGAGTCTGACAAG+AGG | - | chr3.1:91137515-91137534 | MS.gene52784:CDS | 45.0% | |
| CCTCTTGTCAGACTCTTCAT+AGG | + | chr3.1:91137518-91137537 | None:intergenic | 45.0% | |
| CGAAGATGATGTCAAGCATG+AGG | - | chr3.1:91137792-91137811 | MS.gene52784:CDS | 45.0% | |
| TGGAATGGCAAGAATGAGCA+TGG | - | chr3.1:91138135-91138154 | MS.gene52784:CDS | 45.0% | |
| CGGAGATGATGCAAAGCATG+AGG | - | chr3.1:91137570-91137589 | MS.gene52784:CDS | 50.0% | |
| GTTAGAAACCCCTTCCTCAC+CGG | - | chr3.1:91137699-91137718 | MS.gene52784:CDS | 50.0% | |
| TTTAGCAGCGGACAAGCACT+TGG | - | chr3.1:91137640-91137659 | MS.gene52784:CDS | 50.0% | |
| !! | GAAAAGCATTGGGTGGTCAC+CGG | - | chr3.1:91137860-91137879 | MS.gene52784:CDS | 50.0% |
| GTGAGATCTCCGGTGAGGAA+GGG | + | chr3.1:91137711-91137730 | None:intergenic | 55.0% | |
| TGAGATCTCCGGTGAGGAAG+GGG | + | chr3.1:91137710-91137729 | None:intergenic | 55.0% | |
| ! | ATTCGGTGAGATCTCCGGTG+AGG | + | chr3.1:91137716-91137735 | None:intergenic | 55.0% |
| AGCAGCGGACAAGCACTTGG+TGG | - | chr3.1:91137643-91137662 | MS.gene52784:CDS | 60.0% | |
| GGTGAGATCTCCGGTGAGGA+AGG | + | chr3.1:91137712-91137731 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 91137481 | 91138242 | 91137481 | ID=MS.gene52784 |
| chr3.1 | mRNA | 91137481 | 91138242 | 91137481 | ID=MS.gene52784.t1;Parent=MS.gene52784 |
| chr3.1 | exon | 91137481 | 91138242 | 91137481 | ID=MS.gene52784.t1.exon1;Parent=MS.gene52784.t1 |
| chr3.1 | CDS | 91137481 | 91138242 | 91137481 | ID=cds.MS.gene52784.t1;Parent=MS.gene52784.t1 |
| Gene Sequence |
| Protein sequence |
