AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53240.t1 MTR_0558s0020 88.312 231 27 0 1 231 66 296 8.61e-153 445
MS.gene53240.t1 MTR_6g090505 68.667 300 69 2 1 300 1 275 3.88e-142 420
MS.gene53240.t1 MTR_6g090505 67.647 102 6 2 332 431 230 306 3.46e-34 134
MS.gene53240.t1 MTR_6g090505 77.551 49 11 0 503 551 314 362 8.75e-12 67.8
MS.gene53240.t1 MTR_1g083640 89.706 204 21 0 1 204 81 284 6.80e-136 401
MS.gene53240.t1 MTR_8g446020 86.829 205 27 0 27 231 52 256 1.08e-131 389
MS.gene53240.t1 MTR_0337s0060 59.494 316 28 4 383 695 21 239 9.21e-111 335
MS.gene53240.t1 MTR_0337s0040 78.010 191 42 0 5 195 31 221 3.00e-105 320
MS.gene53240.t1 MTR_2g014100 86.047 172 24 0 1 172 74 245 3.35e-105 321
MS.gene53240.t1 MTR_1g055240 88.028 142 17 0 27 168 3 144 1.85e-87 271
MS.gene53240.t1 MTR_0019s0290 82.237 152 27 0 18 169 9 160 9.75e-87 270
MS.gene53240.t1 MTR_3g028680 81.457 151 25 1 554 701 1 151 4.81e-81 256
MS.gene53240.t1 MTR_3g008240 85.507 138 20 0 1 138 66 203 3.96e-80 254
MS.gene53240.t1 MTR_4g040430 84.783 138 17 1 1 138 65 198 2.30e-78 249
MS.gene53240.t1 MTR_2g067090 78.378 148 29 1 557 701 99 246 4.52e-72 236
MS.gene53240.t1 MTR_4g133495 63.536 181 40 2 512 689 88 245 3.59e-68 225
MS.gene53240.t1 MTR_4g133340 63.536 181 40 2 512 689 88 245 3.59e-68 225
MS.gene53240.t1 MTR_5g071590 79.612 103 21 0 1 103 78 180 3.85e-56 189
MS.gene53240.t1 MTR_6g051425 33.333 273 154 5 21 277 64 324 2.27e-36 140
MS.gene53240.t1 MTR_1281s0010 79.365 63 13 0 1 63 47 109 1.95e-28 110
MS.gene53240.t1 MTR_2g067130 62.162 74 22 2 631 701 46 116 2.11e-18 83.2
MS.gene53240.t1 MTR_1g070355 25.357 280 193 4 21 298 119 384 7.32e-16 81.6
MS.gene53240.t1 MTR_0337s0050 75.000 36 9 0 195 230 14 49 1.14e-13 67.0
MS.gene53240.t1 MTR_0402s0040 26.000 150 96 3 20 169 71 205 1.74e-12 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53240.t1 AT4G38180 27.674 589 350 12 20 601 118 637 1.40e-66 234
MS.gene53240.t1 AT4G15090 25.309 648 408 14 59 689 121 709 1.12e-58 213
MS.gene53240.t1 AT4G15090 25.309 648 408 14 59 689 121 709 1.12e-58 213
MS.gene53240.t1 AT4G15090 25.309 648 408 14 59 689 121 709 1.12e-58 213
MS.gene53240.t1 AT4G15090 25.309 648 408 14 59 689 121 709 1.12e-58 213
MS.gene53240.t1 AT4G15090 25.309 648 408 14 59 689 121 709 1.12e-58 213
MS.gene53240.t1 AT2G27110 26.131 597 367 15 63 655 108 634 2.63e-52 195
MS.gene53240.t1 AT2G27110 26.131 597 367 15 63 655 108 634 2.63e-52 195
MS.gene53240.t1 AT2G27110 27.112 509 314 11 149 655 36 489 5.20e-52 192
MS.gene53240.t1 AT1G76320 24.883 643 391 18 19 655 41 597 2.80e-47 178
MS.gene53240.t1 AT3G06250 26.180 466 284 10 135 597 295 703 3.29e-47 179
MS.gene53240.t1 AT3G06250 26.180 466 284 10 135 597 295 703 3.29e-47 179
MS.gene53240.t1 AT3G06250 26.180 466 284 10 135 597 295 703 3.29e-47 179
MS.gene53240.t1 AT1G76320 24.883 643 391 18 19 655 41 597 5.04e-47 178
MS.gene53240.t1 AT1G76320 24.883 643 391 18 19 655 41 597 5.04e-47 178
MS.gene53240.t1 AT1G76320 24.883 643 391 18 19 655 41 597 6.70e-47 178
MS.gene53240.t1 AT3G22170 22.665 653 401 16 4 644 99 659 4.86e-44 170
MS.gene53240.t1 AT3G22170 22.665 653 401 16 4 644 99 659 4.86e-44 170
MS.gene53240.t1 AT4G38170 25.290 431 264 11 171 598 24 399 3.56e-43 164
MS.gene53240.t1 AT5G18960 26.327 471 281 12 134 597 316 727 5.87e-41 160
MS.gene53240.t1 AT5G18960 26.327 471 281 12 134 597 316 727 5.87e-41 160
MS.gene53240.t1 AT1G10240 25.044 567 347 16 43 595 102 604 2.49e-40 158
MS.gene53240.t1 AT1G10240 25.044 567 347 16 43 595 102 604 2.49e-40 158
MS.gene53240.t1 AT5G28530 23.902 615 366 18 5 596 84 619 3.25e-37 149
MS.gene53240.t1 AT2G32250 23.900 682 391 18 2 679 65 622 5.31e-37 148
MS.gene53240.t1 AT2G32250 23.900 682 391 18 2 679 65 622 7.37e-37 148
MS.gene53240.t1 AT2G32250 23.900 682 391 18 2 679 65 622 7.37e-37 148
MS.gene53240.t1 AT2G32250 23.900 682 391 18 2 679 65 622 7.37e-37 148
MS.gene53240.t1 AT2G32250 23.900 682 391 18 2 679 24 581 7.49e-37 148
MS.gene53240.t1 AT2G32250 23.900 682 391 18 2 679 24 581 7.62e-37 148
MS.gene53240.t1 AT2G32250 23.900 682 391 18 2 679 65 622 7.80e-37 148
MS.gene53240.t1 AT2G32250 23.900 682 391 18 2 679 65 622 7.80e-37 148
MS.gene53240.t1 AT5G28530 23.740 615 363 19 5 596 84 615 2.63e-36 145
MS.gene53240.t1 AT5G28530 23.740 615 363 19 5 596 84 615 2.63e-36 145
MS.gene53240.t1 AT1G80010 25.328 458 288 13 146 598 253 661 2.26e-34 140
MS.gene53240.t1 AT1G52520 21.906 598 395 15 2 598 112 638 5.15e-30 127
MS.gene53240.t1 AT4G19990 28.358 268 185 4 155 419 163 426 4.24e-26 114
MS.gene53240.t1 AT4G19990 35.338 133 72 3 527 657 495 615 9.13e-13 72.4
MS.gene53240.t1 AT4G19990 28.358 268 185 4 155 419 106 369 4.94e-26 114
MS.gene53240.t1 AT4G19990 35.338 133 72 3 527 657 438 558 1.00e-12 72.0
MS.gene53240.t1 AT4G19990 28.358 268 185 4 155 419 163 426 5.01e-26 114
MS.gene53240.t1 AT4G19990 35.338 133 72 3 527 657 495 615 9.06e-13 72.4
MS.gene53240.t1 AT4G19990 28.358 268 185 4 155 419 163 426 5.01e-26 114
MS.gene53240.t1 AT4G19990 35.338 133 72 3 527 657 495 615 9.06e-13 72.4
MS.gene53240.t1 AT4G19990 28.358 268 185 4 155 419 177 440 5.39e-26 114
MS.gene53240.t1 AT4G19990 35.338 133 72 3 527 657 509 629 1.07e-12 72.0
MS.gene53240.t1 AT4G19990 28.358 268 185 4 155 419 163 426 6.24e-26 114
MS.gene53240.t1 AT4G19990 35.338 133 72 3 527 657 495 615 9.63e-13 72.0
MS.gene53240.t1 AT4G19990 28.358 268 185 4 155 419 163 426 6.24e-26 114
MS.gene53240.t1 AT4G19990 35.338 133 72 3 527 657 495 615 9.63e-13 72.0
MS.gene53240.t1 AT5G28530 21.167 600 338 19 5 596 84 556 5.67e-18 88.6
MS.gene53240.t1 AT5G28530 21.000 600 335 20 5 596 84 552 3.36e-17 86.3

Find 106 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGCTGAGTTGGACTTTA+AGG 0.133287 3.3:+982525 MS.gene53240:CDS
TTTCGATGAGTTAACTAATT+TGG 0.156761 3.3:+982216 MS.gene53240:CDS
AGTCGCTTCTTTGACCTAAT+TGG 0.186128 3.3:-983351 None:intergenic
CTTTAGTCATTTCAACATTA+TGG 0.194287 3.3:+982483 MS.gene53240:CDS
CAACAAGATTATGCTCTATT+TGG 0.198611 3.3:+981432 MS.gene53240:CDS
TTCATTTCAGCTGCCTTTCT+TGG 0.201003 3.3:+981935 MS.gene53240:intron
CATCGTAAGAGTGTGTTATT+TGG 0.276792 3.3:+981968 MS.gene53240:CDS
TATGGTTTGAGGTTTCTTAT+TGG 0.280533 3.3:-982062 None:intergenic
TTCTTTGACCTAATTGGATT+TGG 0.299426 3.3:-983345 None:intergenic
AATCAGATTTCGACTACAAT+TGG 0.318540 3.3:+982257 MS.gene53240:CDS
TGTTCTTGAGGCAGGGAAAA+AGG 0.322946 3.3:+983197 MS.gene53240:CDS
GCCGATATTTATTGTTTGTT+CGG 0.341758 3.3:-981482 None:intergenic
GGTTTCGAATGTTGATGTTT+TGG 0.354913 3.3:+982780 MS.gene53240:CDS
ACTAGCCTGGTCTGTGTATA+TGG 0.360230 3.3:-982080 None:intergenic
TATGCATTGGTCTTCAGTTT+GGG 0.370760 3.3:+982357 MS.gene53240:CDS
CTCAACGGTGTTCTTGAGGC+AGG 0.386173 3.3:+983189 MS.gene53240:CDS
AACAATAAATATCGGCCATT+AGG 0.388497 3.3:+981489 MS.gene53240:CDS
ACCGAACAAACAATAAATAT+CGG 0.389395 3.3:+981481 MS.gene53240:CDS
GTATGCATTGGTCTTCAGTT+TGG 0.392120 3.3:+982356 MS.gene53240:CDS
GTCTTTAGTGCCAACATTCA+TGG 0.395146 3.3:-983073 None:intergenic
TTCTCATACTCAACAAGAAC+TGG 0.395633 3.3:-980952 None:intergenic
AAGAAAGACCAAATCCAATT+AGG 0.397771 3.3:+983337 MS.gene53240:CDS
TTTGGTCTTTCTTTGAACTT+TGG 0.398856 3.3:-983327 None:intergenic
TTCCTTCAAATACTATTATG+AGG 0.399583 3.3:+982657 MS.gene53240:CDS
GAGGAGTTTCTTATTTCTAA+AGG 0.403538 3.3:+983222 MS.gene53240:CDS
CATGTCGTATGTTTGAGAAC+CGG 0.411671 3.3:+982905 MS.gene53240:CDS
GATTATGATCTTCAAACCTT+GGG 0.413114 3.3:-982828 None:intergenic
GGGTTGTCACAGTCGACATT+AGG 0.419786 3.3:+982863 MS.gene53240:CDS
CGATTATGATCTTCAAACCT+TGG 0.425681 3.3:-982829 None:intergenic
CGAGAAGTAAATGAAGTTCA+AGG 0.430014 3.3:+981129 MS.gene53240:CDS
AGCTGGAATATATTTGAAGT+TGG 0.433136 3.3:+981011 MS.gene53240:CDS
TGGTCTGTGTATATGGTTTG+AGG 0.447350 3.3:-982073 None:intergenic
GAAAGGGTTGGTTGTAAAGC+TGG 0.447509 3.3:+980994 MS.gene53240:CDS
CGCTTTAAAACATATTGTGC+AGG 0.447633 3.3:-983006 None:intergenic
CGTTATGCTTGTAACAAAGA+AGG 0.449439 3.3:+980877 MS.gene53240:CDS
GCTTTAATAAATTCTAAAGC+TGG 0.450264 3.3:+981159 MS.gene53240:CDS
TGGTCTTTGCTCATGGCACA+TGG 0.452802 3.3:+982147 MS.gene53240:CDS
ATGTCGTATGTTTGAGAACC+GGG 0.461344 3.3:+982906 MS.gene53240:CDS
ACAAAATAAGAAATCGGATA+AGG 0.469178 3.3:+980843 MS.gene53240:CDS
GAAGTGATGGGTCAAGGAGT+TGG 0.484292 3.3:+981204 MS.gene53240:CDS
CTCCTCATAATAGTATTTGA+AGG 0.485089 3.3:-982659 None:intergenic
TTACAACCAACCCTTTCCTC+TGG 0.485330 3.3:-980988 None:intergenic
TCTAATGACAACTCGTCAAA+AGG 0.488137 3.3:+981272 MS.gene53240:CDS
CTCAAGTAGCACAAATGCTC+TGG 0.489977 3.3:-981099 None:intergenic
CAATTCACAAAATAAGAAAT+CGG 0.490263 3.3:+980837 MS.gene53240:CDS
TATGAGGAGGACACAACAAA+AGG 0.490908 3.3:+982673 MS.gene53240:CDS
ATGATTGTCAAAACCAAGAA+AGG 0.491378 3.3:-981948 None:intergenic
CTGTCTATGAACCATCTGTT+GGG 0.491412 3.3:+983298 MS.gene53240:CDS
ATCAGATTTCGACTACAATT+GGG 0.491708 3.3:+982258 MS.gene53240:CDS
CGTCAAAAGGAGATGGTGGC+TGG 0.492100 3.3:+981285 MS.gene53240:CDS
ACATTATGGTTCAGAGAATG+AGG 0.494022 3.3:+982497 MS.gene53240:CDS
GACCGCATAAGCTGACTGTT+GGG 0.496307 3.3:-982577 None:intergenic
GAGAACATCTTATGAAGTGA+TGG 0.499875 3.3:+981191 MS.gene53240:CDS
TGTCTTCTGCAGCATCAACT+TGG 0.502977 3.3:-981377 None:intergenic
AAATGCTCTGGTTTATGTAA+TGG 0.504865 3.3:-981087 None:intergenic
CTCATTCAAATCGTATGCAT+TGG 0.507227 3.3:+982344 MS.gene53240:CDS
ATCATAATCGACTTGATGAA+AGG 0.507955 3.3:+982842 MS.gene53240:CDS
TTTAAGTCCGAAAATCGAAA+AGG 0.508080 3.3:-981243 None:intergenic
GAGGGATAATCATGCTGAGT+TGG 0.508852 3.3:+982516 MS.gene53240:CDS
ATTGGTCTTCAGTTTGGGTG+AGG 0.509410 3.3:+982362 MS.gene53240:CDS
TGTCTATAAGATATATAAGT+GGG 0.514682 3.3:+981047 MS.gene53240:CDS
GATCTTCAAACCTTGGGGAA+AGG 0.519004 3.3:-982822 None:intergenic
TAAAGAATCGAACAAGTGAA+TGG 0.519618 3.3:-982465 None:intergenic
TCATAATCGACTTGATGAAA+GGG 0.526860 3.3:+982843 MS.gene53240:CDS
CATAATAGTATTTGAAGGAA+AGG 0.529686 3.3:-982654 None:intergenic
ACTTCAACAACGCACTAGCC+TGG 0.534735 3.3:-982093 None:intergenic
TAACAAAGAAGGTTTCAAGA+AGG 0.538422 3.3:+980888 MS.gene53240:CDS
AATCGAACAAGTGAATGGTC+TGG 0.540971 3.3:-982460 None:intergenic
ATGAGGAGGACACAACAAAA+GGG 0.542065 3.3:+982674 MS.gene53240:CDS
AACAAAGAAGGTTTCAAGAA+GGG 0.545537 3.3:+980889 MS.gene53240:CDS
CTGAAATCCACCATGAATGT+TGG 0.557140 3.3:+983063 MS.gene53240:CDS
AATGAAGCTCTCTCGAAACC+AGG 0.565261 3.3:+981339 MS.gene53240:CDS
GCTGTCTATGAACCATCTGT+TGG 0.568181 3.3:+983297 MS.gene53240:CDS
GTGATGGGTCAAGGAGTTGG+AGG 0.568802 3.3:+981207 MS.gene53240:CDS
TACACGTGTTTGCGCAGATG+CGG 0.571672 3.3:+983149 MS.gene53240:CDS
ATGTCTATAAGATATATAAG+TGG 0.572287 3.3:+981046 MS.gene53240:CDS
GGACCGCATAAGCTGACTGT+TGG 0.579264 3.3:-982578 None:intergenic
TTTGGTGCAGTCCCAACAGA+TGG 0.581566 3.3:-983309 None:intergenic
TGGTGTATACTTGTTTACTA+CGG 0.585960 3.3:-982599 None:intergenic
TCTTATGAAGTGATGGGTCA+AGG 0.589029 3.3:+981198 MS.gene53240:CDS
AGACACTTAAAGTCCGAAGT+AGG 0.589084 3.3:-982313 None:intergenic
TCAACGGTGTTCTTGAGGCA+GGG 0.590097 3.3:+983190 MS.gene53240:CDS
ATTTCTCAACGGTGTTCTTG+AGG 0.591378 3.3:+983185 MS.gene53240:CDS
TTGAAGAAAACCTTTCCCCA+AGG 0.594796 3.3:+982812 MS.gene53240:CDS
TGAAGACTTAGAAGGCGTAA+CGG 0.598405 3.3:-983265 None:intergenic
GACAACTCGTCAAAAGGAGA+TGG 0.599038 3.3:+981278 MS.gene53240:CDS
TTGATATCGTTGTGCTTGAG+TGG 0.601464 3.3:-983097 None:intergenic
AGAACATCTTATGAAGTGAT+GGG 0.601988 3.3:+981192 MS.gene53240:CDS
AACTCGTCAAAAGGAGATGG+TGG 0.603142 3.3:+981281 MS.gene53240:CDS
ACGTGTTTGCGCAGATGCGG+AGG 0.605934 3.3:+983152 MS.gene53240:CDS
ATCCCAACAGTCAGCTTATG+CGG 0.606153 3.3:+982575 MS.gene53240:CDS
CTTCAAATACTATTATGAGG+AGG 0.607664 3.3:+982660 MS.gene53240:CDS
AGAACTGGACTGTCACATGT+TGG 0.620791 3.3:-980937 None:intergenic
GGGTCAAGGAGTTGGAGGCA+CGG 0.621882 3.3:+981212 MS.gene53240:CDS
ATTATGATCTTCAAACCTTG+GGG 0.630358 3.3:-982827 None:intergenic
GGTCTTTGCTCATGGCACAT+GGG 0.639768 3.3:+982148 MS.gene53240:CDS
TTTAGTGCCAACATTCATGG+TGG 0.641441 3.3:-983070 None:intergenic
CATTATGGTTCAGAGAATGA+GGG 0.643278 3.3:+982498 MS.gene53240:CDS
GATTTCAGCTTATCGACAGA+CGG 0.644926 3.3:-983048 None:intergenic
TGAATAAATGAAAAGCAAGA+TGG 0.655697 3.3:-982619 None:intergenic
GCATCTGAAATATTTCTCAA+CGG 0.661104 3.3:+983174 MS.gene53240:CDS
CATGTCGACATAAGAAACCC+CGG 0.663279 3.3:-982924 None:intergenic
TGTCGTATGTTTGAGAACCG+GGG 0.673760 3.3:+982907 MS.gene53240:CDS
ATGTTGATAATGATAATGAG+AGG 0.683310 3.3:+982758 MS.gene53240:CDS
TCAAACCATATACACAGACC+AGG 0.683597 3.3:+982075 MS.gene53240:CDS
TGTTGATAATGATAATGAGA+GGG 0.689129 3.3:+982759 MS.gene53240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTCTATAAGATATATAAG+TGG + chr3.3:981046-981065 MS.gene53240:CDS 20.0%
!! CAATTCACAAAATAAGAAAT+CGG + chr3.3:980837-980856 MS.gene53240:CDS 20.0%
!! TGTCTATAAGATATATAAGT+GGG + chr3.3:981047-981066 MS.gene53240:CDS 20.0%
!!! AATTTAATGTTCTGGTTTAT+GGG + chr3.3:981664-981683 MS.gene53240:intron 20.0%
!!! ATGATGAAAAACTGTTTTAA+TGG + chr3.3:982286-982305 MS.gene53240:CDS 20.0%
!!! TGAATGTTTATATTGATTGA+AGG + chr3.3:981636-981655 MS.gene53240:intron 20.0%
! ACAAAATAAGAAATCGGATA+AGG + chr3.3:980843-980862 MS.gene53240:CDS 25.0%
! ACCGAACAAACAATAAATAT+CGG + chr3.3:981481-981500 MS.gene53240:CDS 25.0%
! AGTACAAAATACTAACCTAA+TGG - chr3.3:981507-981526 None:intergenic 25.0%
! CATAATAGTATTTGAAGGAA+AGG - chr3.3:982657-982676 None:intergenic 25.0%
! CTTTAGTCATTTCAACATTA+TGG + chr3.3:982483-982502 MS.gene53240:CDS 25.0%
! TGAAATTTGAATGAATGTTC+TGG + chr3.3:981554-981573 MS.gene53240:intron 25.0%
! TGAATAAATGAAAAGCAAGA+TGG - chr3.3:982622-982641 None:intergenic 25.0%
! TTCCTTCAAATACTATTATG+AGG + chr3.3:982657-982676 MS.gene53240:CDS 25.0%
! TTTCGATGAGTTAACTAATT+TGG + chr3.3:982216-982235 MS.gene53240:CDS 25.0%
!! AGGTTTAGAATTTAATGTTC+TGG + chr3.3:981656-981675 MS.gene53240:intron 25.0%
!! TCAATTTCGAATGTTTTTCA+AGG + chr3.3:982115-982134 MS.gene53240:CDS 25.0%
!! TGTTGTATTTTGTTCTGATT+TGG + chr3.3:981912-981931 MS.gene53240:intron 25.0%
!!! ATGTTGATAATGATAATGAG+AGG + chr3.3:982758-982777 MS.gene53240:CDS 25.0%
!!! GAATTTAATGTTCTGGTTTA+TGG + chr3.3:981663-981682 MS.gene53240:intron 25.0%
!!! GCTTTAATAAATTCTAAAGC+TGG + chr3.3:981159-981178 MS.gene53240:CDS 25.0%
!!! TGAAAAACTGTTTTAATGGA+AGG + chr3.3:982290-982309 MS.gene53240:CDS 25.0%
!!! TGTTGATAATGATAATGAGA+GGG + chr3.3:982759-982778 MS.gene53240:CDS 25.0%
AAACACATATGATCTTTCCA+TGG - chr3.3:981727-981746 None:intergenic 30.0%
AACAAAGAAGGTTTCAAGAA+GGG + chr3.3:980889-980908 MS.gene53240:CDS 30.0%
AACAATAAATATCGGCCATT+AGG + chr3.3:981489-981508 MS.gene53240:CDS 30.0%
AAGAAAGACCAAATCCAATT+AGG + chr3.3:983337-983356 MS.gene53240:CDS 30.0%
AATCAGATTTCGACTACAAT+TGG + chr3.3:982257-982276 MS.gene53240:CDS 30.0%
AGAACATCTTATGAAGTGAT+GGG + chr3.3:981192-981211 MS.gene53240:CDS 30.0%
ATCAGATTTCGACTACAATT+GGG + chr3.3:982258-982277 MS.gene53240:CDS 30.0%
ATGATTGTCAAAACCAAGAA+AGG - chr3.3:981951-981970 None:intergenic 30.0%
ATTATGATCTTCAAACCTTG+GGG - chr3.3:982830-982849 None:intergenic 30.0%
CAACAAGATTATGCTCTATT+TGG + chr3.3:981432-981451 MS.gene53240:CDS 30.0%
CTCCTCATAATAGTATTTGA+AGG - chr3.3:982662-982681 None:intergenic 30.0%
CTGGTATATGTGTGTTAATT+TGG + chr3.3:981593-981612 MS.gene53240:intron 30.0%
CTTCAAATACTATTATGAGG+AGG + chr3.3:982660-982679 MS.gene53240:CDS 30.0%
GAGAATGCAAAGAAAAATCT+CGG + chr3.3:982172-982191 MS.gene53240:CDS 30.0%
GATTATGATCTTCAAACCTT+GGG - chr3.3:982831-982850 None:intergenic 30.0%
GCCGATATTTATTGTTTGTT+CGG - chr3.3:981485-981504 None:intergenic 30.0%
TAAAGAAAAACCAGAGGAAA+GGG + chr3.3:980978-980997 MS.gene53240:CDS 30.0%
TAAAGAATCGAACAAGTGAA+TGG - chr3.3:982468-982487 None:intergenic 30.0%
TAACAAAGAAGGTTTCAAGA+AGG + chr3.3:980888-980907 MS.gene53240:CDS 30.0%
TGGATATCATAGTAAAAACC+TGG - chr3.3:981360-981379 None:intergenic 30.0%
TGGTGTATACTTGTTTACTA+CGG - chr3.3:982602-982621 None:intergenic 30.0%
TGTATAGAGCATAAATTCCA+AGG - chr3.3:981703-981722 None:intergenic 30.0%
TTCTTTGACCTAATTGGATT+TGG - chr3.3:983348-983367 None:intergenic 30.0%
TTTAAGTCCGAAAATCGAAA+AGG - chr3.3:981246-981265 None:intergenic 30.0%
TTTCTATTTACACAGCACAA+TGG + chr3.3:981871-981890 MS.gene53240:intron 30.0%
! AAATGCTCTGGTTTATGTAA+TGG - chr3.3:981090-981109 None:intergenic 30.0%
! AGCTGGAATATATTTGAAGT+TGG + chr3.3:981011-981030 MS.gene53240:CDS 30.0%
! CAAAGTTTTCACAATTGAGA+AGG + chr3.3:982717-982736 MS.gene53240:CDS 30.0%
! GAATCTTCCTTTTCGATTTT+CGG + chr3.3:981236-981255 MS.gene53240:CDS 30.0%
! GCATCTGAAATATTTCTCAA+CGG + chr3.3:983174-983193 MS.gene53240:CDS 30.0%
! TATGGTTTGAGGTTTCTTAT+TGG - chr3.3:982065-982084 None:intergenic 30.0%
! TGAGGCTTTAACAATTTAAG+CGG + chr3.3:983446-983465 MS.gene53240:intron 30.0%
! TTTGGTCTTTCTTTGAACTT+TGG - chr3.3:983330-983349 None:intergenic 30.0%
!! ACATGTTTTCAAGATTTTGG+AGG + chr3.3:982942-982961 MS.gene53240:CDS 30.0%
!! ATCATAATCGACTTGATGAA+AGG + chr3.3:982842-982861 MS.gene53240:CDS 30.0%
!! ATGTTCTGGTTTAGAATTGA+AGG + chr3.3:981568-981587 MS.gene53240:intron 30.0%
!! GAGGAGTTTCTTATTTCTAA+AGG + chr3.3:983222-983241 MS.gene53240:CDS 30.0%
!! TCATAATCGACTTGATGAAA+GGG + chr3.3:982843-982862 MS.gene53240:CDS 30.0%
!! TCGACATGTTTTCAAGATTT+TGG + chr3.3:982939-982958 MS.gene53240:CDS 30.0%
!!! AAGACATAGCCAGTATTTTT+TGG + chr3.3:981394-981413 MS.gene53240:CDS 30.0%
!!! AGACATAGCCAGTATTTTTT+GGG + chr3.3:981395-981414 MS.gene53240:CDS 30.0%
!!! CTGCACAATATGTTTTAAAG+CGG + chr3.3:983007-983026 MS.gene53240:CDS 30.0%
!!! GTTTTTCAAGGTGTTTTTCA+TGG + chr3.3:982127-982146 MS.gene53240:CDS 30.0%
ACATTATGGTTCAGAGAATG+AGG + chr3.3:982497-982516 MS.gene53240:CDS 35.0%
ATTTATGCTCTATACAGCCA+TGG + chr3.3:981707-981726 MS.gene53240:intron 35.0%
CATAAATTCCAAGGCAAGAT+AGG - chr3.3:981694-981713 None:intergenic 35.0%
CATTATGGTTCAGAGAATGA+GGG + chr3.3:982498-982517 MS.gene53240:CDS 35.0%
CGAGAAGTAAATGAAGTTCA+AGG + chr3.3:981129-981148 MS.gene53240:CDS 35.0%
CGATTATGATCTTCAAACCT+TGG - chr3.3:982832-982851 None:intergenic 35.0%
CGTTATGCTTGTAACAAAGA+AGG + chr3.3:980877-980896 MS.gene53240:CDS 35.0%
CTAAAGAAAAACCAGAGGAA+AGG + chr3.3:980977-980996 MS.gene53240:CDS 35.0%
CTCATTCAAATCGTATGCAT+TGG + chr3.3:982344-982363 MS.gene53240:CDS 35.0%
GAAAGCTAAAGAAAAACCAG+AGG + chr3.3:980972-980991 MS.gene53240:CDS 35.0%
GAAGCAAAAGAAAGATGATG+AGG + chr3.3:983428-983447 MS.gene53240:intron 35.0%
GACAGAAATTCCTTATAGCT+CGG + chr3.3:983403-983422 MS.gene53240:intron 35.0%
GAGAACATCTTATGAAGTGA+TGG + chr3.3:981191-981210 MS.gene53240:CDS 35.0%
GTCTGCTTAATCCTAAAAAC+AGG + chr3.3:980914-980933 MS.gene53240:CDS 35.0%
TCTAATGACAACTCGTCAAA+AGG + chr3.3:981272-981291 MS.gene53240:CDS 35.0%
TTCTCATACTCAACAAGAAC+TGG - chr3.3:980955-980974 None:intergenic 35.0%
! CATCGTAAGAGTGTGTTATT+TGG + chr3.3:981968-981987 MS.gene53240:CDS 35.0%
! GAGGCTTTAACAATTTAAGC+GGG + chr3.3:983447-983466 MS.gene53240:intron 35.0%
! GGTTTCGAATGTTGATGTTT+TGG + chr3.3:982780-982799 MS.gene53240:CDS 35.0%
! TCTTTCAATGCTTTTCTTCG+TGG + chr3.3:982427-982446 MS.gene53240:CDS 35.0%
! TGGTTTAGAATTGAAGGTTC+TGG + chr3.3:981574-981593 MS.gene53240:intron 35.0%
! TTTTAATGGAAGGCCTACTT+CGG + chr3.3:982300-982319 MS.gene53240:CDS 35.0%
! TTTTTCATGGTCTTTGCTCA+TGG + chr3.3:982140-982159 MS.gene53240:CDS 35.0%
!! ACTGCTTTTGAAGACTTAGA+AGG - chr3.3:983276-983295 None:intergenic 35.0%
!! CGCTTTAAAACATATTGTGC+AGG - chr3.3:983009-983028 None:intergenic 35.0%
!! TATGCATTGGTCTTCAGTTT+GGG + chr3.3:982357-982376 MS.gene53240:CDS 35.0%
!! TTCAAGATTTTGGAGGTCTT+AGG + chr3.3:982949-982968 MS.gene53240:CDS 35.0%
!!! AGACATCAGCTACTTTTGAT+TGG + chr3.3:982011-982030 MS.gene53240:CDS 35.0%
!!! CACAATATGTTTTAAAGCGG+TGG + chr3.3:983010-983029 MS.gene53240:CDS 35.0%
AATCGAACAAGTGAATGGTC+TGG - chr3.3:982463-982482 None:intergenic 40.0%
ACCTATGATCTTTGCATAGC+TGG - chr3.3:981821-981840 None:intergenic 40.0%
AGACACTTAAAGTCCGAAGT+AGG - chr3.3:982316-982335 None:intergenic 40.0%
AGTCGCTTCTTTGACCTAAT+TGG - chr3.3:983354-983373 None:intergenic 40.0%
ATGAGGAGGACACAACAAAA+GGG + chr3.3:982674-982693 MS.gene53240:CDS 40.0%
ATGTCGTATGTTTGAGAACC+GGG + chr3.3:982906-982925 MS.gene53240:CDS 40.0%
ATTTCTCAACGGTGTTCTTG+AGG + chr3.3:983185-983204 MS.gene53240:CDS 40.0%
CATGTCGTATGTTTGAGAAC+CGG + chr3.3:982905-982924 MS.gene53240:CDS 40.0%
CTGAAATCCACCATGAATGT+TGG + chr3.3:983063-983082 MS.gene53240:CDS 40.0%
CTGTCTATGAACCATCTGTT+GGG + chr3.3:983298-983317 MS.gene53240:CDS 40.0%
GATTTCAGCTTATCGACAGA+CGG - chr3.3:983051-983070 None:intergenic 40.0%
GTCTTTAGTGCCAACATTCA+TGG - chr3.3:983076-983095 None:intergenic 40.0%
TATGAGGAGGACACAACAAA+AGG + chr3.3:982673-982692 MS.gene53240:CDS 40.0%
TCAAACCATATACACAGACC+AGG + chr3.3:982075-982094 MS.gene53240:CDS 40.0%
TCCAGCTATGCAAAGATCAT+AGG + chr3.3:981817-981836 MS.gene53240:intron 40.0%
TCCATCTGCCCAAAAAATAC+TGG - chr3.3:981406-981425 None:intergenic 40.0%
TGGTCTGTGTATATGGTTTG+AGG - chr3.3:982076-982095 None:intergenic 40.0%
TTCATTTCAGCTGCCTTTCT+TGG + chr3.3:981935-981954 MS.gene53240:intron 40.0%
TTGAAGAAAACCTTTCCCCA+AGG + chr3.3:982812-982831 MS.gene53240:CDS 40.0%
TTGATATCGTTGTGCTTGAG+TGG - chr3.3:983100-983119 None:intergenic 40.0%
TTTAGTGCCAACATTCATGG+TGG - chr3.3:983073-983092 None:intergenic 40.0%
TTTGCTTCTTCCGAGCTATA+AGG - chr3.3:983416-983435 None:intergenic 40.0%
! TCATGCTGAGTTGGACTTTA+AGG + chr3.3:982525-982544 MS.gene53240:CDS 40.0%
! TGAAGACTTAGAAGGCGTAA+CGG - chr3.3:983268-983287 None:intergenic 40.0%
!! AAGATTTTGGAGGTCTTAGG+TGG + chr3.3:982952-982971 MS.gene53240:CDS 40.0%
!! GTATGCATTGGTCTTCAGTT+TGG + chr3.3:982356-982375 MS.gene53240:CDS 40.0%
!! TCACATGTTGGCCTGTTTTT+AGG - chr3.3:980928-980947 None:intergenic 40.0%
!! TCTTATGAAGTGATGGGTCA+AGG + chr3.3:981198-981217 MS.gene53240:CDS 40.0%
AACTCGTCAAAAGGAGATGG+TGG + chr3.3:981281-981300 MS.gene53240:CDS 45.0%
AATGAAGCTCTCTCGAAACC+AGG + chr3.3:981339-981358 MS.gene53240:CDS 45.0%
AGAACTGGACTGTCACATGT+TGG - chr3.3:980940-980959 None:intergenic 45.0%
ATCCCAACAGTCAGCTTATG+CGG + chr3.3:982575-982594 MS.gene53240:CDS 45.0%
CATGTCGACATAAGAAACCC+CGG - chr3.3:982927-982946 None:intergenic 45.0%
CTCAAGTAGCACAAATGCTC+TGG - chr3.3:981102-981121 None:intergenic 45.0%
GAAAAACCAGAGGAAAGGGT+TGG + chr3.3:980982-981001 MS.gene53240:CDS 45.0%
GACAACTCGTCAAAAGGAGA+TGG + chr3.3:981278-981297 MS.gene53240:CDS 45.0%
GAGGGATAATCATGCTGAGT+TGG + chr3.3:982516-982535 MS.gene53240:CDS 45.0%
GATCTTCAAACCTTGGGGAA+AGG - chr3.3:982825-982844 None:intergenic 45.0%
GCTGTCTATGAACCATCTGT+TGG + chr3.3:983297-983316 MS.gene53240:CDS 45.0%
TGTCGTATGTTTGAGAACCG+GGG + chr3.3:982907-982926 MS.gene53240:CDS 45.0%
TGTCTTCTGCAGCATCAACT+TGG - chr3.3:981380-981399 None:intergenic 45.0%
TGTTCTTGAGGCAGGGAAAA+AGG + chr3.3:983197-983216 MS.gene53240:CDS 45.0%
TTACAACCAACCCTTTCCTC+TGG - chr3.3:980991-981010 None:intergenic 45.0%
! ACTAGCCTGGTCTGTGTATA+TGG - chr3.3:982083-982102 None:intergenic 45.0%
! GTGAGGTCACATTTTACAGC+TGG + chr3.3:982379-982398 MS.gene53240:CDS 45.0%
!! ATTGGTCTTCAGTTTGGGTG+AGG + chr3.3:982362-982381 MS.gene53240:CDS 45.0%
!! GAAAGGGTTGGTTGTAAAGC+TGG + chr3.3:980994-981013 MS.gene53240:CDS 45.0%
!!! GCCAGTATTTTTTGGGCAGA+TGG + chr3.3:981402-981421 MS.gene53240:CDS 45.0%
ACTTCAACAACGCACTAGCC+TGG - chr3.3:982096-982115 None:intergenic 50.0%
GACCGCATAAGCTGACTGTT+GGG - chr3.3:982580-982599 None:intergenic 50.0%
GGGTTGTCACAGTCGACATT+AGG + chr3.3:982863-982882 MS.gene53240:CDS 50.0%
GGTCTTTGCTCATGGCACAT+GGG + chr3.3:982148-982167 MS.gene53240:CDS 50.0%
TACACGTGTTTGCGCAGATG+CGG + chr3.3:983149-983168 MS.gene53240:CDS 50.0%
TCAACGGTGTTCTTGAGGCA+GGG + chr3.3:983190-983209 MS.gene53240:CDS 50.0%
TGGGTGATCCTATCTTGCCT+TGG + chr3.3:981683-981702 MS.gene53240:intron 50.0%
TGGTCTTTGCTCATGGCACA+TGG + chr3.3:982147-982166 MS.gene53240:CDS 50.0%
! TTTGGTGCAGTCCCAACAGA+TGG - chr3.3:983312-983331 None:intergenic 50.0%
!! GAAGTGATGGGTCAAGGAGT+TGG + chr3.3:981204-981223 MS.gene53240:CDS 50.0%
CTCAACGGTGTTCTTGAGGC+AGG + chr3.3:983189-983208 MS.gene53240:CDS 55.0%
GGACCGCATAAGCTGACTGT+TGG - chr3.3:982581-982600 None:intergenic 55.0%
TGAGGCAGGGAAAAAGGCAG+AGG + chr3.3:983203-983222 MS.gene53240:CDS 55.0%
! CGTCAAAAGGAGATGGTGGC+TGG + chr3.3:981285-981304 MS.gene53240:CDS 55.0%
!! GTGATGGGTCAAGGAGTTGG+AGG + chr3.3:981207-981226 MS.gene53240:CDS 55.0%
ACGTGTTTGCGCAGATGCGG+AGG + chr3.3:983152-983171 MS.gene53240:CDS 60.0%
GGGTCAAGGAGTTGGAGGCA+CGG + chr3.3:981212-981231 MS.gene53240:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 980820 983475 980820 ID=MS.gene53240
chr3.3 mRNA 980820 983475 980820 ID=MS.gene53240.t1;Parent=MS.gene53240
chr3.3 exon 980820 981510 980820 ID=MS.gene53240.t1.exon1;Parent=MS.gene53240.t1
chr3.3 CDS 980820 981510 980820 ID=cds.MS.gene53240.t1;Parent=MS.gene53240.t1
chr3.3 exon 981945 983358 981945 ID=MS.gene53240.t1.exon2;Parent=MS.gene53240.t1
chr3.3 CDS 981945 983358 981945 ID=cds.MS.gene53240.t1;Parent=MS.gene53240.t1
chr3.3 exon 983466 983475 983466 ID=MS.gene53240.t1.exon3;Parent=MS.gene53240.t1
chr3.3 CDS 983466 983475 983466 ID=cds.MS.gene53240.t1;Parent=MS.gene53240.t1
Gene Sequence

>MS.gene53240

ATGTTGAAGTTCAAGAACAATTCACAAAATAAGAAATCGGATAAGGTACATTATGTGCGTTATGCTTGTAACAAAGAAGGTTTCAAGAAGGGAAGTCTGCTTAATCCTAAAAACAGGCCAACATGTGACAGTCCAGTTCTTGTTGAGTATGAGAAAGCTAAAGAAAAACCAGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGTTGGATGAAGCGTTGAATGTCTATAAGATATATAAGTGGGAAGTTGATCACTGTCATCCATTACATAAACCAGAGCATTTGTGCTACTTGAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTTGCTTTAATAAATTCTAAAGCTGGAATGTCGATGAGAACATCTTATGAAGTGATGGGTCAAGGAGTTGGAGGCACGGAGAATCTTCCTTTTCGATTTTCGGACTTAAAGAACTATCTAATGACAACTCGTCAAAAGGAGATGGTGGCTGGTGAAGCAACTGTTATTCAAGAATTCTTTAGAAATGAAGCTCTCTCGAAACCAGGTTTTTACTATGATATCCAAGTTGATGCTGCAGAAGACATAGCCAGTATTTTTTGGGCAGATGGAATTATGCAACAAGATTATGCTCTATTTGGTGATGTTGTCAGTTTTGATACAACTTACCGAACAAACAATAAATATCGGCCATTAGGTTAGTATTTTGTACTTCTCACAGTTTTTAATTCTTATATTCTTGAAATTTGAATGAATGTTCTGGTTTAGAATTGAAGGTTCTGGTATATGTGTGTTAATTTGGCTCTTGTGTGTGTTTAGAATTGAATGTTTATATTGATTGAAGGTTTAGAATTTAATGTTCTGGTTTATGGGTGATCCTATCTTGCCTTGGAATTTATGCTCTATACAGCCATGGAAAGATCATATGTGTTTTTTTATCGATGATAAATAGCTCTTTCTGTGTTTGCGAGTTTAGACATCTCGCTTGAGAAGTTGTGCTATGTCCAGCTATGCAAAGATCATAGGTGTTTTTTTACAGTTGTTAAATGAATAGCTTTTTCTATTTACACAGCACAATGGTTTTATATTTTTTAAATATGTTGTATTTTGTTCTGATTTGGTTCATTTCAGCTGCCTTTCTTGGTTTTGACAATCATCGTAAGAGTGTGTTATTTGGTGCTGCGCTACTGTATGATGAGACATCAGCTACTTTTGATTGGTTGTTTACAACTTTTTTGAAGTGTATGTCCAATAAGAAACCTCAAACCATATACACAGACCAGGCTAGTGCGTTGTTGAAGTCAATTTCGAATGTTTTTCAAGGTGTTTTTCATGGTCTTTGCTCATGGCACATGGGAGAGAATGCAAAGAAAAATCTCGGCTCTCGTGCAAACAGTGCATTTTTCGATGAGTTAACTAATTTGGTTTCAAATGTCGAAGATGAATCAGATTTCGACTACAATTGGGATCAGATGATGAAAAACTGTTTTAATGGAAGGCCTACTTCGGACTTTAAGTGTCTTGTTCAAACTCATTCAAATCGTATGCATTGGTCTTCAGTTTGGGTGAGGTCACATTTTACAGCTGGTTTGAAAACGACACAGTTAAGTGAGTCTTTCAATGCTTTTCTTCGTGGATTTTTGCAGCCAGACCATTCACTTGTTCGATTCTTTAGTCATTTCAACATTATGGTTCAGAGAATGAGGGATAATCATGCTGAGTTGGACTTTAAGGCTGCAAACACTAGAACAAAAAATAATTATCCCAACAGTCAGCTTATGCGGTCCGTAGTAAACAAGTATACACCATCTTGCTTTTCATTTATTCACAGTCAGTATGACCTTTCCTTCAAATACTATTATGAGGAGGACACAACAAAAGGGTCTGAATTGAACAAGTTCTTCAAAGTTTTCACAATTGAGAAGGTTGATTATAATTACGATGATGTTGATAATGATAATGAGAGGGTTTCGAATGTTGATGTTTTGGATGCAGAATTTGAAGAAAACCTTTCCCCAAGGTTTGAAGATCATAATCGACTTGATGAAAGGGTTGTCACAGTCGACATTAGGAGCAAAAGTTTTACTTGTTCATGTCGTATGTTTGAGAACCGGGGTTTCTTATGTCGACATGTTTTCAAGATTTTGGAGGTCTTAGGTGGTCATGTGCAATATCATTTTTTGAAGACTATACCTGCACAATATGTTTTAAAGCGGTGGACTAGAGATGTGCGTCCGTCTGTCGATAAGCTGAAATCCACCATGAATGTTGGCACTAAAGACACCACTCAAGCACAACGATATCAACAAATTTGTGCTGTTACAGTTCAGCTTTCTACACGTGTTTGCGCAGATGCGGAGGCATCTGAAATATTTCTCAACGGTGTTCTTGAGGCAGGGAAAAAGGCAGAGGAGTTTCTTATTTCTAAAGGTATTCATACAGATCGATCATCCGTTACGCCTTCTAAGTCTTCAAAAGCAGTTGCTGTCTATGAACCATCTGTTGGGACTGCACCAAAGTTCAAAGAAAGACCAAATCCAATTAGGTCAAAGAAGCGACTTAAAAGTGATTATGAAAAAGCTAGAGCAAGACAGAAATTCCTTATAGCTCGGAAGAAGCAAAAGAAAGATGATGAGGCTTTAACAATTTAAGCGGGCACTGA
Protein sequence

>MS.gene53240.t1

MLKFKNNSQNKKSDKVHYVRYACNKEGFKKGSLLNPKNRPTCDSPVLVEYEKAKEKPEERVGCKAGIYLKLDEALNVYKIYKWEVDHCHPLHKPEHLCYLRSFREVNEVQGQLALINSKAGMSMRTSYEVMGQGVGGTENLPFRFSDLKNYLMTTRQKEMVAGEATVIQEFFRNEALSKPGFYYDIQVDAAEDIASIFWADGIMQQDYALFGDVVSFDTTYRTNNKYRPLAAFLGFDNHRKSVLFGAALLYDETSATFDWLFTTFLKCMSNKKPQTIYTDQASALLKSISNVFQGVFHGLCSWHMGENAKKNLGSRANSAFFDELTNLVSNVEDESDFDYNWDQMMKNCFNGRPTSDFKCLVQTHSNRMHWSSVWVRSHFTAGLKTTQLSESFNAFLRGFLQPDHSLVRFFSHFNIMVQRMRDNHAELDFKAANTRTKNNYPNSQLMRSVVNKYTPSCFSFIHSQYDLSFKYYYEEDTTKGSELNKFFKVFTIEKVDYNYDDVDNDNERVSNVDVLDAEFEENLSPRFEDHNRLDERVVTVDIRSKSFTCSCRMFENRGFLCRHVFKILEVLGGHVQYHFLKTIPAQYVLKRWTRDVRPSVDKLKSTMNVGTKDTTQAQRYQQICAVTVQLSTRVCADAEASEIFLNGVLEAGKKAEEFLISKGIHTDRSSVTPSKSSKAVAVYEPSVGTAPKFKERPNPISGH