AlfalfaGEDB | MS.gene53293

M. sativa cultivar XinJiangDaYe / MS.gene53293

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF13912.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53293.t1	XP_013455142.1	90.5	126	11	1	1	125	1	126	5.60E-54	220.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53293.t1	Q9M202	44.4	117	36	6	2	115	1	91	3.3e-10	66.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53293.t1	A0A072UTH2	90.5	126	11	1	1	125	1	126	4.1e-54	220.3

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene53293.t1	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53293.t1	MTR_4g027440	91.200	125	10	1	1	124	1	125	5.39e-63	197
MS.gene53293.t1	MTR_0160s0030	57.895	133	28	7	2	114	1	125	6.21e-29	108
MS.gene53293.t1	MTR_4g100860	44.737	114	61	2	2	113	1	114	1.24e-21	88.6
MS.gene53293.t1	MTR_5g012630	41.727	139	54	7	2	122	1	130	3.26e-20	84.0
MS.gene53293.t1	MTR_4g109340	45.070	71	39	0	4	74	10	80	8.42e-14	67.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53293.t1	AT4G16610	40.179	112	55	3	1	112	1	100	3.07e-16	72.4
MS.gene53293.t1	AT1G02030	40.367	109	26	4	1	109	1	70	6.38e-13	64.3
MS.gene53293.t1	AT3G60580	38.793	116	48	6	2	117	1	93	3.33e-11	59.7
MS.gene53293.t1	AT2G45120	66.667	33	11	0	2	34	1	33	5.72e-11	59.3

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Find 26 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTGGCCCACGATCCAAAC+GGG	0.477505	4.3:+71344576	MS.gene53293:CDS
ACAGTTGGTGCTGCTCGCGG+CGG	0.667210	4.3:-71344388	None:intergenic
ACCAGCGTCGATGAATTGAT+TGG	0.461470	4.3:-71344500	None:intergenic
ATCAACTTTCTTGACCCGTT+TGG	0.394557	4.3:-71344590	None:intergenic
CAACTGGCCCACGATCCAAA+CGG	0.561624	4.3:+71344575	MS.gene53293:CDS
CCAGCGTCGATGAATTGATT+GGG	0.505485	4.3:-71344499	None:intergenic
CCCAATCAATTCATCGACGC+TGG	0.575794	4.3:+71344499	MS.gene53293:CDS
CGTTTGGATCGTGGGCCAGT+TGG	0.485267	4.3:-71344574	None:intergenic
GAGTCCTCAAAGAATCCAAC+TGG	0.487670	4.3:+71344559	MS.gene53293:CDS
GCTCATTGCTCAGCAAATTG+GGG	0.586512	4.3:+71345520	MS.gene53293:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATAAACAAGTTGCAGAAAG+TGG	+	chr4.3:71345009-71345028	MS.gene53293:intron	30.0%
CATAGGAGAACACATAATAT+TGG	+	chr4.3:71345174-71345193	MS.gene53293:intron	30.0%
GAATTTGTCAAACCTCATTA+AGG	+	chr4.3:71345402-71345421	MS.gene53293:intron	30.0%
AGAACTAAAACCAGTGAAGA+AGG	+	chr4.3:71344900-71344919	MS.gene53293:intron	35.0%
AGGTTTGACAAATTCAGCAT+CGG	-	chr4.3:71345397-71345416	None:intergenic	35.0%
TCAGCAAATTGGGGTTATAA+TGG	+	chr4.3:71345529-71345548	MS.gene53293:CDS	35.0%
TGAACAGTATGAACAACAAC+AGG	+	chr4.3:71344795-71344814	MS.gene53293:intron	35.0%
TTCTGTACTGAGTTCTTCAT+CGG	+	chr4.3:71344645-71344664	MS.gene53293:intron	35.0%
TTTCGTTCATATCAAGCACT+AGG	+	chr4.3:71344961-71344980	MS.gene53293:intron	35.0%
AAACAAGTTGCAGAAAGTGG+TGG	+	chr4.3:71345012-71345031	MS.gene53293:intron	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	71344268	71345562	71344268	ID=MS.gene53293
chr4.3	mRNA	71344268	71345562	71344268	ID=MS.gene53293.t1;Parent=MS.gene53293
chr4.3	exon	71344268	71344638	71344268	ID=MS.gene53293.t1.exon1;Parent=MS.gene53293.t1
chr4.3	CDS	71344268	71344638	71344268	ID=cds.MS.gene53293.t1;Parent=MS.gene53293.t1
chr4.3	exon	71345505	71345562	71345505	ID=MS.gene53293.t1.exon2;Parent=MS.gene53293.t1
chr4.3	CDS	71345505	71345562	71345505	ID=cds.MS.gene53293.t1;Parent=MS.gene53293.t1

Gene Sequence

>MS.gene53293

ATGATGGAGAAACACAAGTGCAAATTTTGCTTGAGAAGTTTTTCCAATGGTAGAGCTTTAGGTGGACACATGAGATCACACATGATGAACCTACCAAAACAAACTGAGTCATCACCACCACCGCCGCGAGCAGCACCAACTGTTCAGCTCAGTTTCGAAGCTGAATCAGCTTCATCTCTTTCATCCTATGGTCTAAGAGAGAATCCAAAGCGAAGCTTTCGATTCACAGATCCCAATCAATTCATCGACGCTGGTTCGATAATTCATCAAGAAGATAGAGAAAGTGAAACTGAGTCCTCAAAGAATCCAACTGGCCCACGATCCAAACGGGTCAAGAAAGTTGATCCTATGAAAAACTCAGTACAGACTGAGTCTGTTTCTGTACTGAGTTCTTCATCGGATATAACAACTGAAGAAGATGTTGCTTTTTTTCTCATATCACTGTCTCGCGAAGATAACAACATATGGAAAACTCATACACAACATCATGATCAACAACATCAGCAGCATCATGAAGAACAAGAAGATGAACAGTATGAACAACAACAGGTCGAAGAAGATGAAGACCAAGAAGATGAAGATGTTGAGGAAGCAGAAGAAGAAAGTGAAGCTGAATCTGAAGAAGAAGAAGAAGAACTAAAACCAGTGAAGAAGGTACGAGGAAGATACAAATGTGACACGTGTAACAAAGTGTTTCGTTCATATCAAGCACTAGGTGGACACAGAGCGAGTCACAAGAAAAATAAACAAGTTGCAGAAAGTGGTGGTGATTATTCAATTTCACAACATCATGAGAAAATTAACATTGAAAAAAATATCAATGTTAATAATAATAAGAGTGAAAAGAAAATTCATGAATGTCCAATTTGTTTTAGAGAATTTTCTTCAGGTCAAGCACTAGGTGGACATAGGAGAACACATAATATTGGATTCGGATCAGCATCAACATCAGCAGCAGCAACAACAACAACGATGGTTAGAACAAACGTTGAGGTTGCTCGAGGTTCCGCGAATGTTGGAGATAGTTTGTTAGATCTAAATTTTCCTGCTCCGATGGACGAAGATGAAGATGAAGACGAAGATGATGGTGGTGACGATGTTAGTCAAGTTGAAGGTTCTGCTGTTTCCGATGCTGAATTTGTCAAACCTCATTAAGGTTGAAAAAGAAAATGATGAATTCATTTTTTATTTAGTTTGATGGATCCAAAGGTACAAAATTTTAGTTCTGTTTTTGCAGAACAATTTTAGGTTTGCTCATTGCTCAGCAAATTGGGGTTATAATGGTTATTTCATAG

Protein sequence

>MS.gene53293.t1

MMEKHKCKFCLRSFSNGRALGGHMRSHMMNLPKQTESSPPPPRAAPTVQLSFEAESASSLSSYGLRENPKRSFRFTDPNQFIDAGSIIHQEDRESETESSKNPTGPRSKRVKKVDPMKNSVQTETILGLLIAQQIGVIMVIS