Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53293.t1 | XP_013455142.1 | 90.5 | 126 | 11 | 1 | 1 | 125 | 1 | 126 | 5.60E-54 | 220.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53293.t1 | Q9M202 | 44.4 | 117 | 36 | 6 | 2 | 115 | 1 | 91 | 3.3e-10 | 66.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53293.t1 | A0A072UTH2 | 90.5 | 126 | 11 | 1 | 1 | 125 | 1 | 126 | 4.1e-54 | 220.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene53293.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53293.t1 | MTR_4g027440 | 91.200 | 125 | 10 | 1 | 1 | 124 | 1 | 125 | 5.39e-63 | 197 |
| MS.gene53293.t1 | MTR_0160s0030 | 57.895 | 133 | 28 | 7 | 2 | 114 | 1 | 125 | 6.21e-29 | 108 |
| MS.gene53293.t1 | MTR_4g100860 | 44.737 | 114 | 61 | 2 | 2 | 113 | 1 | 114 | 1.24e-21 | 88.6 |
| MS.gene53293.t1 | MTR_5g012630 | 41.727 | 139 | 54 | 7 | 2 | 122 | 1 | 130 | 3.26e-20 | 84.0 |
| MS.gene53293.t1 | MTR_4g109340 | 45.070 | 71 | 39 | 0 | 4 | 74 | 10 | 80 | 8.42e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53293.t1 | AT4G16610 | 40.179 | 112 | 55 | 3 | 1 | 112 | 1 | 100 | 3.07e-16 | 72.4 |
| MS.gene53293.t1 | AT1G02030 | 40.367 | 109 | 26 | 4 | 1 | 109 | 1 | 70 | 6.38e-13 | 64.3 |
| MS.gene53293.t1 | AT3G60580 | 38.793 | 116 | 48 | 6 | 2 | 117 | 1 | 93 | 3.33e-11 | 59.7 |
| MS.gene53293.t1 | AT2G45120 | 66.667 | 33 | 11 | 0 | 2 | 34 | 1 | 33 | 5.72e-11 | 59.3 |
Find 26 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTCATTGCTCAGCAAATT+GGG | 0.202212 | 4.3:+71345519 | MS.gene53293:CDS |
| TTGCTCATTGCTCAGCAAAT+TGG | 0.257224 | 4.3:+71345518 | MS.gene53293:CDS |
| TCAGCAAATTGGGGTTATAA+TGG | 0.370820 | 4.3:+71345529 | MS.gene53293:CDS |
| ATCAACTTTCTTGACCCGTT+TGG | 0.394557 | 4.3:-71344590 | None:intergenic |
| GGTGATGACTCAGTTTGTTT+TGG | 0.396035 | 4.3:-71344361 | None:intergenic |
| GTGAATCGAAAGCTTCGCTT+TGG | 0.412150 | 4.3:-71344472 | None:intergenic |
| GCTTCATCTCTTTCATCCTA+TGG | 0.420837 | 4.3:+71344436 | MS.gene53293:CDS |
| TGGGCCAGTTGGATTCTTTG+AGG | 0.421097 | 4.3:-71344563 | None:intergenic |
| ACCAGCGTCGATGAATTGAT+TGG | 0.461470 | 4.3:-71344500 | None:intergenic |
| GTTGGTGCTGCTCGCGGCGG+TGG | 0.464935 | 4.3:-71344385 | None:intergenic |
| TGGATTCTCTCTTAGACCAT+AGG | 0.471463 | 4.3:-71344452 | None:intergenic |
| AACTGGCCCACGATCCAAAC+GGG | 0.477505 | 4.3:+71344576 | MS.gene53293:CDS |
| CGTTTGGATCGTGGGCCAGT+TGG | 0.485267 | 4.3:-71344574 | None:intergenic |
| GAGTCCTCAAAGAATCCAAC+TGG | 0.487670 | 4.3:+71344559 | MS.gene53293:CDS |
| TCTTGACCCGTTTGGATCGT+GGG | 0.488842 | 4.3:-71344582 | None:intergenic |
| TTCTTGACCCGTTTGGATCG+TGG | 0.496085 | 4.3:-71344583 | None:intergenic |
| GGTGCTGCTCGCGGCGGTGG+TGG | 0.503220 | 4.3:-71344382 | None:intergenic |
| CCAGCGTCGATGAATTGATT+GGG | 0.505485 | 4.3:-71344499 | None:intergenic |
| TCCACCTAAAGCTCTACCAT+TGG | 0.552290 | 4.3:-71344311 | None:intergenic |
| CAACTGGCCCACGATCCAAA+CGG | 0.561624 | 4.3:+71344575 | MS.gene53293:CDS |
| TCGAAACTGAGCTGAACAGT+TGG | 0.568717 | 4.3:-71344403 | None:intergenic |
| CCCAATCAATTCATCGACGC+TGG | 0.575794 | 4.3:+71344499 | MS.gene53293:CDS |
| GCTCATTGCTCAGCAAATTG+GGG | 0.586512 | 4.3:+71345520 | MS.gene53293:CDS |
| TCCAATGGTAGAGCTTTAGG+TGG | 0.603163 | 4.3:+71344310 | MS.gene53293:CDS |
| TGAACAGTTGGTGCTGCTCG+CGG | 0.604272 | 4.3:-71344391 | None:intergenic |
| ACAGTTGGTGCTGCTCGCGG+CGG | 0.667210 | 4.3:-71344388 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCATTTTTTATTTAGTTTGA+TGG | + | chr4.3:71345446-71345465 | MS.gene53293:intron | 15.0% |
| !!! | GAAAATTCTCTAAAACAAAT+TGG | - | chr4.3:71345132-71345151 | None:intergenic | 20.0% |
| ! | AGAACACATAATATTGGATT+CGG | + | chr4.3:71345180-71345199 | MS.gene53293:intron | 25.0% |
| !! | GAACTAAAATTTTGTACCTT+TGG | - | chr4.3:71345474-71345493 | None:intergenic | 25.0% |
| !!! | TGTTTTAGAGAATTTTCTTC+AGG | + | chr4.3:71345135-71345154 | MS.gene53293:intron | 25.0% |
| !!! | TGTTTTTGCAGAACAATTTT+AGG | + | chr4.3:71345494-71345513 | MS.gene53293:intron | 25.0% |
| !!! | TTTCTTTTTCAACCTTAATG+AGG | - | chr4.3:71345417-71345436 | None:intergenic | 25.0% |
| AATAAACAAGTTGCAGAAAG+TGG | + | chr4.3:71345009-71345028 | MS.gene53293:intron | 30.0% | |
| CATAGGAGAACACATAATAT+TGG | + | chr4.3:71345174-71345193 | MS.gene53293:intron | 30.0% | |
| GAATTTGTCAAACCTCATTA+AGG | + | chr4.3:71345402-71345421 | MS.gene53293:intron | 30.0% | |
| !! | ATTTAGTTTGATGGATCCAA+AGG | + | chr4.3:71345455-71345474 | MS.gene53293:intron | 30.0% |
| AGAACTAAAACCAGTGAAGA+AGG | + | chr4.3:71344900-71344919 | MS.gene53293:intron | 35.0% | |
| AGGTTTGACAAATTCAGCAT+CGG | - | chr4.3:71345397-71345416 | None:intergenic | 35.0% | |
| TCAGCAAATTGGGGTTATAA+TGG | + | chr4.3:71345529-71345548 | MS.gene53293:CDS | 35.0% | |
| TGAACAGTATGAACAACAAC+AGG | + | chr4.3:71344795-71344814 | MS.gene53293:intron | 35.0% | |
| TTCTGTACTGAGTTCTTCAT+CGG | + | chr4.3:71344645-71344664 | MS.gene53293:intron | 35.0% | |
| TTTCGTTCATATCAAGCACT+AGG | + | chr4.3:71344961-71344980 | MS.gene53293:intron | 35.0% | |
| ! | GTCTGTACTGAGTTTTTCAT+AGG | - | chr4.3:71344616-71344635 | None:intergenic | 35.0% |
| ! | TGCTTGAGAAGTTTTTCCAA+TGG | + | chr4.3:71344295-71344314 | MS.gene53293:CDS | 35.0% |
| ! | TTTTCCAATGGTAGAGCTTT+AGG | + | chr4.3:71344307-71344326 | MS.gene53293:CDS | 35.0% |
| !!! | ATGACTCAGTTTGTTTTGGT+AGG | - | chr4.3:71344360-71344379 | None:intergenic | 35.0% |
| AAACAAGTTGCAGAAAGTGG+TGG | + | chr4.3:71345012-71345031 | MS.gene53293:intron | 40.0% | |
| ATCAACTTTCTTGACCCGTT+TGG | - | chr4.3:71344593-71344612 | None:intergenic | 40.0% | |
| CAAACTATCTCCAACATTCG+CGG | - | chr4.3:71345277-71345296 | None:intergenic | 40.0% | |
| CCAAGAAGATGAAGATGTTG+AGG | + | chr4.3:71344834-71344853 | MS.gene53293:intron | 40.0% | |
| CCTCAACATCTTCATCTTCT+TGG | - | chr4.3:71344837-71344856 | None:intergenic | 40.0% | |
| CTCGCGAAGATAACAACATA+TGG | + | chr4.3:71344713-71344732 | MS.gene53293:intron | 40.0% | |
| GAAGATGAAGACGAAGATGA+TGG | + | chr4.3:71345333-71345352 | MS.gene53293:intron | 40.0% | |
| GATGGTTAGAACAAACGTTG+AGG | + | chr4.3:71345239-71345258 | MS.gene53293:intron | 40.0% | |
| GCTTCATCTCTTTCATCCTA+TGG | + | chr4.3:71344436-71344455 | MS.gene53293:CDS | 40.0% | |
| TCATCTTCATCTTCGTCCAT+CGG | - | chr4.3:71345321-71345340 | None:intergenic | 40.0% | |
| TGCTCATTGCTCAGCAAATT+GGG | + | chr4.3:71345519-71345538 | MS.gene53293:CDS | 40.0% | |
| TGGATTCTCTCTTAGACCAT+AGG | - | chr4.3:71344455-71344474 | None:intergenic | 40.0% | |
| TTGCTCATTGCTCAGCAAAT+TGG | + | chr4.3:71345518-71345537 | MS.gene53293:CDS | 40.0% | |
| ! | TCTAAATTTTCCTGCTCCGA+TGG | + | chr4.3:71345302-71345321 | MS.gene53293:intron | 40.0% |
| ! | TTTTCTTCAGGTCAAGCACT+AGG | + | chr4.3:71345147-71345166 | MS.gene53293:intron | 40.0% |
| !! | GACGATGTTAGTCAAGTTGA+AGG | + | chr4.3:71345360-71345379 | MS.gene53293:intron | 40.0% |
| !! | GGTGATGACTCAGTTTGTTT+TGG | - | chr4.3:71344364-71344383 | None:intergenic | 40.0% |
| AGCAGCAACAACAACAACGA+TGG | + | chr4.3:71345221-71345240 | MS.gene53293:intron | 45.0% | |
| CGTTCATATCAAGCACTAGG+TGG | + | chr4.3:71344964-71344983 | MS.gene53293:intron | 45.0% | |
| GAGTCCTCAAAGAATCCAAC+TGG | + | chr4.3:71344559-71344578 | MS.gene53293:CDS | 45.0% | |
| GCTCATTGCTCAGCAAATTG+GGG | + | chr4.3:71345520-71345539 | MS.gene53293:CDS | 45.0% | |
| GTGAATCGAAAGCTTCGCTT+TGG | - | chr4.3:71344475-71344494 | None:intergenic | 45.0% | |
| TCCACCTAAAGCTCTACCAT+TGG | - | chr4.3:71344314-71344333 | None:intergenic | 45.0% | |
| TCGAAACTGAGCTGAACAGT+TGG | - | chr4.3:71344406-71344425 | None:intergenic | 45.0% | |
| ! | AAACCAGTGAAGAAGGTACG+AGG | + | chr4.3:71344907-71344926 | MS.gene53293:intron | 45.0% |
| ! | GATGAAGACGAAGATGATGG+TGG | + | chr4.3:71345336-71345355 | MS.gene53293:intron | 45.0% |
| !! | ACCAGCGTCGATGAATTGAT+TGG | - | chr4.3:71344503-71344522 | None:intergenic | 45.0% |
| !! | CCAGCGTCGATGAATTGATT+GGG | - | chr4.3:71344502-71344521 | None:intergenic | 45.0% |
| !! | TCCAATGGTAGAGCTTTAGG+TGG | + | chr4.3:71344310-71344329 | MS.gene53293:CDS | 45.0% |
| ACAAACGTTGAGGTTGCTCG+AGG | + | chr4.3:71345249-71345268 | MS.gene53293:intron | 50.0% | |
| CCCAATCAATTCATCGACGC+TGG | + | chr4.3:71344499-71344518 | MS.gene53293:CDS | 50.0% | |
| CTTCCTCGTACCTTCTTCAC+TGG | - | chr4.3:71344913-71344932 | None:intergenic | 50.0% | |
| GTCAAGCACTAGGTGGACAT+AGG | + | chr4.3:71345157-71345176 | MS.gene53293:intron | 50.0% | |
| TCTTCAGGTCAAGCACTAGG+TGG | + | chr4.3:71345150-71345169 | MS.gene53293:intron | 50.0% | |
| TCTTGACCCGTTTGGATCGT+GGG | - | chr4.3:71344585-71344604 | None:intergenic | 50.0% | |
| TGGGCCAGTTGGATTCTTTG+AGG | - | chr4.3:71344566-71344585 | None:intergenic | 50.0% | |
| TTCTTGACCCGTTTGGATCG+TGG | - | chr4.3:71344586-71344605 | None:intergenic | 50.0% | |
| AACTGGCCCACGATCCAAAC+GGG | + | chr4.3:71344576-71344595 | MS.gene53293:CDS | 55.0% | |
| CAACTGGCCCACGATCCAAA+CGG | + | chr4.3:71344575-71344594 | MS.gene53293:CDS | 55.0% | |
| TCATCTTCGTCCATCGGAGC+AGG | - | chr4.3:71345315-71345334 | None:intergenic | 55.0% | |
| !! | TGAACAGTTGGTGCTGCTCG+CGG | - | chr4.3:71344394-71344413 | None:intergenic | 55.0% |
| CGTTTGGATCGTGGGCCAGT+TGG | - | chr4.3:71344577-71344596 | None:intergenic | 60.0% | |
| GCTCGAGGTTCCGCGAATGT+TGG | + | chr4.3:71345264-71345283 | MS.gene53293:intron | 60.0% | |
| !! | ACAGTTGGTGCTGCTCGCGG+CGG | - | chr4.3:71344391-71344410 | None:intergenic | 65.0% |
| !! | GTTGGTGCTGCTCGCGGCGG+TGG | - | chr4.3:71344388-71344407 | None:intergenic | 75.0% |
| !!! | GGTGCTGCTCGCGGCGGTGG+TGG | - | chr4.3:71344385-71344404 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 71344268 | 71345562 | 71344268 | ID=MS.gene53293 |
| chr4.3 | mRNA | 71344268 | 71345562 | 71344268 | ID=MS.gene53293.t1;Parent=MS.gene53293 |
| chr4.3 | exon | 71344268 | 71344638 | 71344268 | ID=MS.gene53293.t1.exon1;Parent=MS.gene53293.t1 |
| chr4.3 | CDS | 71344268 | 71344638 | 71344268 | ID=cds.MS.gene53293.t1;Parent=MS.gene53293.t1 |
| chr4.3 | exon | 71345505 | 71345562 | 71345505 | ID=MS.gene53293.t1.exon2;Parent=MS.gene53293.t1 |
| chr4.3 | CDS | 71345505 | 71345562 | 71345505 | ID=cds.MS.gene53293.t1;Parent=MS.gene53293.t1 |
| Gene Sequence |
| Protein sequence |
