AlfalfaGEDB | MS.gene53294

M. sativa cultivar XinJiangDaYe / MS.gene53294

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53294.t1	XP_003605334.1	98.1	159	3	0	1	159	1	159	3.30E-79	304.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53294.t1	Q9FJK3	46.2	156	83	1	1	155	1	156	3.5e-29	129.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53294.t1	G7JJI5	98.1	159	3	0	1	159	1	159	2.4e-79	304.3

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene53294.t1	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53294.t1	MTR_4g028720	98.113	159	3	0	1	159	1	159	3.00e-114	320
MS.gene53294.t1	MTR_7g011950	91.824	159	13	0	1	159	1	159	1.46e-99	283
MS.gene53294.t1	MTR_1g077320	86.164	159	22	0	1	159	1	159	6.38e-92	264
MS.gene53294.t1	MTR_5g047580	71.154	156	45	0	1	156	1	156	1.64e-77	227
MS.gene53294.t1	MTR_1g077390	70.701	157	46	0	1	157	1	157	1.94e-77	227
MS.gene53294.t1	MTR_1g084950	76.282	156	37	0	1	156	1	156	3.46e-76	224
MS.gene53294.t1	MTR_1g077300	74.843	159	38	1	1	159	1	157	2.22e-73	217
MS.gene53294.t1	MTR_5g047560	59.615	156	47	1	1	156	1	140	1.71e-58	179
MS.gene53294.t1	MTR_1g090697	55.484	155	69	0	1	155	1	155	8.51e-58	177
MS.gene53294.t1	MTR_1g090710	53.205	156	72	1	1	155	1	156	9.57e-56	172

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53294.t1	AT5G48670	46.405	153	81	1	1	152	1	153	1.45e-33	120
MS.gene53294.t1	AT3G05860	38.926	149	90	1	1	148	1	149	9.96e-31	110
MS.gene53294.t1	AT3G05860	38.926	149	90	1	1	148	1	149	5.33e-30	109
MS.gene53294.t1	AT5G26630	38.750	160	97	1	1	159	1	160	7.61e-30	108
MS.gene53294.t1	AT3G05860	38.926	149	90	1	1	148	1	149	8.03e-30	109
MS.gene53294.t1	AT1G65300	36.620	142	89	1	3	143	2	143	1.27e-27	104
MS.gene53294.t1	AT1G65330	36.735	147	92	1	3	148	2	148	4.79e-27	102
MS.gene53294.t1	AT5G26650	33.987	153	100	1	4	155	2	154	1.93e-24	97.4
MS.gene53294.t1	AT5G27960	33.333	153	101	1	4	155	2	154	3.46e-23	93.2
MS.gene53294.t1	AT1G31630	29.677	155	107	2	3	156	2	155	8.36e-22	89.7

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Find 25 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCCAAACAGATGATTGAG+AGG	0.630265	4.3:+71275962	MS.gene53294:CDS
ACCTACAAGCGGAGGAAGAA+AGG	0.494539	4.3:+71275832	MS.gene53294:CDS
ACCTTTCTTCCTCCGCTTGT+AGG	0.316491	4.3:-71275833	None:intergenic
AGAGAACTCACTATTCTTTG+TGG	0.526508	4.3:+71275871	MS.gene53294:CDS
AGAGCTCATATATGAAAGAT+GGG	0.560272	4.3:+71275989	MS.gene53294:CDS
AGAGGTGTGGCCAAATCTAA+AGG	0.522555	4.3:+71275939	MS.gene53294:CDS
AGCTGAACATTCTTATGATT+TGG	0.274450	4.3:+71276106	MS.gene53294:CDS
ATACCTCTCAATCATCTGTT+TGG	0.362514	4.3:-71275965	None:intergenic
CAAAGTCATGGCTAGGAAGA+AGG	0.502352	4.3:+71275768	None:intergenic
CAAGAAAGGCGACCTACAAG+CGG	0.654193	4.3:+71275821	MS.gene53294:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAGCTCATATATGAAAGAT+GGG	+	chr4.3:71275989-71276008	MS.gene53294:CDS	30.0%
AGCTGAACATTCTTATGATT+TGG	+	chr4.3:71276106-71276125	MS.gene53294:CDS	30.0%
GCAAAAGATTACTAAAGCTA+GGG	+	chr4.3:71276044-71276063	MS.gene53294:CDS	30.0%
TGCAAAAGATTACTAAAGCT+AGG	+	chr4.3:71276043-71276062	MS.gene53294:CDS	30.0%
TTTGCAAAAGGAAACTTTCT+TGG	-	chr4.3:71276029-71276048	None:intergenic	30.0%
AGAGAACTCACTATTCTTTG+TGG	+	chr4.3:71275871-71275890	MS.gene53294:CDS	35.0%
ATACCTCTCAATCATCTGTT+TGG	-	chr4.3:71275968-71275987	None:intergenic	35.0%
CAGAGCTCATATATGAAAGA+TGG	+	chr4.3:71275988-71276007	MS.gene53294:CDS	35.0%
CTCCAATGATTCAACAAGAA+AGG	+	chr4.3:71275807-71275826	MS.gene53294:CDS	35.0%
TCAGAAACAGTCACATCATT+TGG	-	chr4.3:71276153-71276172	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	71275775	71276254	71275775	ID=MS.gene53294
chr4.3	mRNA	71275775	71276254	71275775	ID=MS.gene53294.t1;Parent=MS.gene53294
chr4.3	exon	71275775	71276254	71275775	ID=MS.gene53294.t1.exon1;Parent=MS.gene53294.t1
chr4.3	CDS	71275775	71276254	71275775	ID=cds.MS.gene53294.t1;Parent=MS.gene53294.t1

Gene Sequence

>MS.gene53294

ATGGCTAGGAAGAAGGTGAAACTCGCTTTTATCTCCAATGATTCAACAAGAAAGGCGACCTACAAGCGGAGGAAGAAAGGTATCATCAAGAAGGTGAGAGAACTCACTATTCTTTGTGGTATTCCAGCATGTGCTATAATTTCCGATCCTTTTGATTCTAAAACAGAGGTGTGGCCAAATCTAAAGGAAGCCAAACAGATGATTGAGAGGTATCAGAGCTCATATATGAAAGATGGGAGAAAAAATGTGAACCAAGAAAGTTTCCTTTTGCAAAAGATTACTAAAGCTAGGGAGCAACTGAGAATACAAAGACACGACAATCATGAGAATGAGCTGAACATTCTTATGATTTGGTACCTACAAAACAACAAGCTGCCAAATGATGTGACTGTTTCTGATTTGAAAGACTTGGATAAGTTGATTGAAAAAAATATGAAGGAAATTGATGACAAGATGGCTTCACTTAGTTTATCAAACTGA

Protein sequence

>MS.gene53294.t1

MARKKVKLAFISNDSTRKATYKRRKKGIIKKVRELTILCGIPACAIISDPFDSKTEVWPNLKEAKQMIERYQSSYMKDGRKNVNQESFLLQKITKAREQLRIQRHDNHENELNILMIWYLQNNKLPNDVTVSDLKDLDKLIEKNMKEIDDKMASLSLSN