AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53329

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53329.t1 MTR_8g019540 91.875 320 22 3 1 318 1 318 0.0 561
MS.gene53329.t1 MTR_2g099490 53.659 328 117 10 7 318 23 331 2.40e-91 275
MS.gene53329.t1 MTR_2g099490 51.613 341 120 10 7 318 23 347 1.35e-89 271
MS.gene53329.t1 MTR_2g041090 48.404 188 86 6 111 287 17 204 2.45e-45 154
MS.gene53329.t1 MTR_2g030170 63.333 90 32 1 138 227 85 173 8.37e-34 123
MS.gene53329.t1 MTR_2g030170 68.354 79 25 0 149 227 128 206 1.12e-32 121
MS.gene53329.t1 MTR_8g037270 47.651 149 60 5 149 280 136 283 1.86e-31 119
MS.gene53329.t1 MTR_3g061510 54.902 102 36 2 125 222 131 226 4.26e-31 119
MS.gene53329.t1 MTR_3g061510 54.902 102 36 2 125 222 151 246 5.40e-31 119
MS.gene53329.t1 MTR_1g056530 49.573 117 44 4 112 226 120 223 8.49e-27 108
MS.gene53329.t1 MTR_7g106450 49.153 118 45 4 112 227 122 226 2.22e-26 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53329.t1 AT3G14020 45.425 306 130 14 4 294 17 300 1.02e-58 191
MS.gene53329.t1 AT3G14020 45.425 306 130 14 4 294 17 300 1.02e-58 191
MS.gene53329.t1 AT1G72830 37.273 330 176 9 4 316 23 338 5.79e-51 172
MS.gene53329.t1 AT1G72830 37.160 331 176 10 4 316 23 339 3.81e-49 167
MS.gene53329.t1 AT1G17590 43.651 252 109 9 79 316 94 326 1.98e-47 162
MS.gene53329.t1 AT1G17590 43.651 252 109 9 79 316 94 326 1.98e-47 162
MS.gene53329.t1 AT1G17590 43.651 252 109 9 79 316 94 326 1.98e-47 162
MS.gene53329.t1 AT1G17590 43.651 252 109 9 79 316 94 326 1.98e-47 162
MS.gene53329.t1 AT1G54160 46.639 238 105 9 7 230 12 241 1.04e-46 160
MS.gene53329.t1 AT1G72830 33.435 329 165 9 4 316 23 313 7.84e-37 134
MS.gene53329.t1 AT1G30500 55.556 108 47 1 124 231 58 164 8.82e-37 130
MS.gene53329.t1 AT1G30500 53.704 108 45 1 124 231 58 160 5.82e-35 125
MS.gene53329.t1 AT2G34720 47.899 119 52 2 115 227 44 158 4.54e-31 116
MS.gene53329.t1 AT2G34720 50.442 113 46 2 121 227 15 123 3.34e-30 112
MS.gene53329.t1 AT2G34720 50.442 113 46 2 121 227 15 123 3.34e-30 112
MS.gene53329.t1 AT5G12840 45.802 131 57 4 104 229 112 233 4.58e-30 115
MS.gene53329.t1 AT5G12840 45.802 131 57 4 104 229 112 233 4.58e-30 115
MS.gene53329.t1 AT5G12840 45.802 131 57 4 104 229 113 234 4.78e-30 115
MS.gene53329.t1 AT5G12840 45.802 131 57 4 104 229 113 234 4.78e-30 115
MS.gene53329.t1 AT5G12840 45.802 131 57 4 104 229 113 234 4.78e-30 115
MS.gene53329.t1 AT3G20910 48.413 126 47 5 106 222 105 221 1.28e-29 115
MS.gene53329.t1 AT3G05690 46.667 135 50 6 109 235 90 210 5.71e-24 99.8
MS.gene53329.t1 AT5G06510 49.138 116 45 5 112 226 88 190 9.44e-24 98.6
MS.gene53329.t1 AT5G06510 49.138 116 45 5 112 226 88 190 9.44e-24 98.6
MS.gene53329.t1 AT5G06510 49.138 116 45 5 112 226 88 190 9.44e-24 98.6
MS.gene53329.t1 AT5G06510 49.138 116 45 5 112 226 88 190 9.44e-24 98.6
MS.gene53329.t1 AT3G05690 46.667 135 50 6 109 235 127 247 1.35e-23 99.4
MS.gene53329.t1 AT5G06510 51.351 111 40 5 117 226 44 141 1.93e-23 96.7
MS.gene53329.t1 AT5G06510 54.369 103 33 5 125 226 6 95 1.96e-23 95.1
MS.gene53329.t1 AT5G06510 54.369 103 33 5 125 226 6 95 1.96e-23 95.1

Find 81 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTATACTGGACAGAAAT+TGG 0.227693 8.4:-73708588 None:intergenic
TGCTTCGCATTCACATAAAT+TGG 0.276367 8.4:-73709537 None:intergenic
AACTGGTCGTCAATCTTATC+TGG 0.313777 8.4:+73707759 MS.gene53329:CDS
TACCTTGCCTCCTTCTCAAC+TGG 0.319759 8.4:+73708695 MS.gene53329:CDS
CGGATCATCTGCAGGGAGTC+AGG 0.383305 8.4:+73708668 MS.gene53329:CDS
ACATTATTCCGTGATCCATA+TGG 0.396988 8.4:-73709020 None:intergenic
ATCTTATCTGGAAGTTGGTT+CGG 0.412123 8.4:+73707771 MS.gene53329:CDS
GGTTCCTCACAAAGATTAAG+AGG 0.418602 8.4:-73709516 None:intergenic
TGTTCAACACTTAGCGAAAC+CGG 0.430982 8.4:+73708621 MS.gene53329:CDS
ATTGAGTAGAAGAAGAATCT+TGG 0.441655 8.4:-73707735 None:intergenic
TGAAGTCGGATTCATGTTGT+TGG 0.453276 8.4:-73709957 None:intergenic
AAACATTAAAGCCATGGTCT+TGG 0.460398 8.4:-73709801 None:intergenic
CATGCTGAGACATACTCTAA+TGG 0.462998 8.4:+73708984 MS.gene53329:CDS
TGGCGCGACTCGTGTAGATA+TGG 0.466406 8.4:-73709718 None:intergenic
AAGCAGAGGAATTGTTATAC+TGG 0.467814 8.4:-73708599 None:intergenic
TGGTTACCCTTCTCATATGC+AGG 0.470689 8.4:+73709987 MS.gene53329:CDS
GGCCTACTAGCAGCTCCATA+TGG 0.471924 8.4:+73709005 MS.gene53329:CDS
TCTTCTTCTACTCAATCAAC+TGG 0.473460 8.4:+73707742 MS.gene53329:CDS
GTGGAAATGTGCTGGAATCA+AGG 0.474923 8.4:+73709848 MS.gene53329:CDS
CTAATCATGTCTCTCACATA+AGG 0.480287 8.4:-73710065 None:intergenic
TTGAAGAGAGCTAGAGGCTC+TGG 0.481702 8.4:+73709751 MS.gene53329:CDS
GGTATCATCGGATCATCTGC+AGG 0.483140 8.4:+73708660 MS.gene53329:CDS
GCCGATGCCGGTGGTGGTGG+TGG 0.483222 8.4:+73710018 MS.gene53329:CDS
TCAGCCGATGCCGGTGGTGG+TGG 0.489227 8.4:+73710015 MS.gene53329:CDS
CGTCAATCTTATCTGGAAGT+TGG 0.507589 8.4:+73707766 MS.gene53329:CDS
AACCTAGTGGTTTAGACTTG+TGG 0.508102 8.4:-73707699 None:intergenic
TTGTCAGCCGATGCCGGTGG+TGG 0.509405 8.4:+73710012 MS.gene53329:CDS
TAGCAGGAATGCCTCCCGTT+CGG 0.509646 8.4:+73709484 MS.gene53329:CDS
ATTCCGACCACCACCACCAC+CGG 0.525135 8.4:-73710025 None:intergenic
CAATGAAACACTCATGGCCA+TGG 0.528292 8.4:+73707593 None:intergenic
CAGGATTTGTCAGCCGATGC+CGG 0.529122 8.4:+73710006 MS.gene53329:CDS
TTAATGTTTCCACCACTACT+CGG 0.534943 8.4:+73709815 MS.gene53329:CDS
TCTCTCACATAAGGACAGAT+AGG 0.538450 8.4:-73710056 None:intergenic
ACCTTCCACACTCTTTCCGC+CGG 0.539251 8.4:-73708640 None:intergenic
GTTCATCATACTCAGCTAGC+AGG 0.544741 8.4:+73709468 MS.gene53329:CDS
AGAGGCAAAGGAATCCGAAC+GGG 0.545487 8.4:-73709498 None:intergenic
TTTGCCTCTTAATCTTTGTG+AGG 0.548183 8.4:+73709512 MS.gene53329:CDS
TGGAAATGTGCTGGAATCAA+GGG 0.553488 8.4:+73709849 MS.gene53329:CDS
GAAGTTGGTTCGGAACAATC+CGG 0.553531 8.4:+73707781 MS.gene53329:CDS
GACAAATCCTGCATATGAGA+AGG 0.557039 8.4:-73709994 None:intergenic
TCACAAAGATTAAGAGGCAA+AGG 0.559654 8.4:-73709510 None:intergenic
ATCCATATGGAGCTGCTAGT+AGG 0.561398 8.4:-73709007 None:intergenic
GAATCCGAGTGGAAATGTGC+TGG 0.564823 8.4:+73709840 MS.gene53329:CDS
CTCTCTTCAATGCATGCAGA+TGG 0.566697 8.4:-73709738 None:intergenic
AATGAAACACTCATGGCCAT+GGG 0.573475 8.4:+73707594 None:intergenic
CGGATTCAGCCGAGTAGTGG+TGG 0.574639 8.4:-73709824 None:intergenic
ACTCGGATTCAGCCGAGTAG+TGG 0.577382 8.4:-73709827 None:intergenic
ACTTGTGACGTCGGAACAAG+TGG 0.585137 8.4:-73709911 None:intergenic
TTAGAGTATGTCTCAGCATG+AGG 0.588380 8.4:-73708981 None:intergenic
CAAGTAGAAAAGTATAGAGA+TGG 0.594749 8.4:+73709877 MS.gene53329:CDS
CGAAACCGGCGGAAAGAGTG+TGG 0.602019 8.4:+73708635 MS.gene53329:CDS
ACCGGCGGAAAGAGTGTGGA+AGG 0.603635 8.4:+73708639 MS.gene53329:CDS
TTGTGATCCAGTTGAGAAGG+AGG 0.605135 8.4:-73708702 None:intergenic
CCTTGAAATTGAAAACCTAG+TGG 0.606654 8.4:-73707712 None:intergenic
AAGAGTGTGGAAGGTATCAT+CGG 0.608613 8.4:+73708648 MS.gene53329:CDS
TGGTGGAAACATTAAAGCCA+TGG 0.608658 8.4:-73709807 None:intergenic
AAGAGAGCTAGAGGCTCTGG+TGG 0.614598 8.4:+73709754 MS.gene53329:CDS
CATGCATTGAAGAGAGCTAG+AGG 0.616928 8.4:+73709745 MS.gene53329:CDS
GATTCCAGCACATTTCCACT+CGG 0.619314 8.4:-73709844 None:intergenic
TGATTGTGATCCAGTTGAGA+AGG 0.622776 8.4:-73708705 None:intergenic
GCTAAGTGTTGAACAAGCAG+AGG 0.624669 8.4:-73708613 None:intergenic
GGAAGACAATGAAACACTCA+TGG 0.629376 8.4:+73707587 None:intergenic
ACAAATCCTGCATATGAGAA+GGG 0.629662 8.4:-73709993 None:intergenic
GATTTGTCAGCCGATGCCGG+TGG 0.632484 8.4:+73710009 MS.gene53329:CDS
TAGCAGCTCCATATGGATCA+CGG 0.633412 8.4:+73709012 MS.gene53329:CDS
GGCAAAGGAATCCGAACGGG+AGG 0.635841 8.4:-73709495 None:intergenic
ATCCAGTTGAGAAGGAGGCA+AGG 0.636669 8.4:-73708697 None:intergenic
TAGAGTATGTCTCAGCATGA+GGG 0.637829 8.4:-73708980 None:intergenic
ATTAGAAGCACTTGTGACGT+CGG 0.640775 8.4:-73709920 None:intergenic
GTCTACGTCTCATAATAGCA+TGG 0.645173 8.4:-73709563 None:intergenic
GAATCCGACTTCAGATCATG+TGG 0.653505 8.4:+73709967 MS.gene53329:CDS
ATGAAACACTCATGGCCATG+GGG 0.654173 8.4:+73707595 MS.gene53329:CDS
AAGAGGCAAAGGAATCCGAA+CGG 0.660223 8.4:-73709499 None:intergenic
GTAACCACATGATCTGAAGT+CGG 0.661286 8.4:-73709971 None:intergenic
GACGTCGGAACAAGTGGTTG+TGG 0.668249 8.4:-73709905 None:intergenic
TGCCACAAGTCTAAACCACT+AGG 0.682061 8.4:+73707697 MS.gene53329:CDS
GTATCATCGGATCATCTGCA+GGG 0.684338 8.4:+73708661 MS.gene53329:CDS
ACTACTCGGCTGAATCCGAG+TGG 0.691698 8.4:+73709829 MS.gene53329:CDS
TGAAACACTCATGGCCATGG+GGG 0.692226 8.4:+73707596 MS.gene53329:CDS
CTCACATAAGGACAGATAGG+CGG 0.718305 8.4:-73710053 None:intergenic
TCAACACTTAGCGAAACCGG+CGG 0.741425 8.4:+73708624 MS.gene53329:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATTAATTTCAATCTT+TGG - chr8.4:73708476-73708495 None:intergenic 10.0%
!!! ACATTATGAATATTTTAAAA+AGG - chr8.4:73708270-73708289 None:intergenic 10.0%
!! ATAATAAGTGGTTAAATATA+AGG + chr8.4:73708150-73708169 MS.gene53329:intron 15.0%
!! TTAACAATTAACTATCAATT+TGG - chr8.4:73708177-73708196 None:intergenic 15.0%
!! AATTACGAATATATAGTTAC+CGG - chr8.4:73707803-73707822 None:intergenic 20.0%
!! AGATAAAGACATAAATATGA+TGG - chr8.4:73709107-73709126 None:intergenic 20.0%
!! ATCATATTTATGTCTTTATC+TGG + chr8.4:73709106-73709125 MS.gene53329:intron 20.0%
!! TACAAAATTAATGACTAACT+TGG - chr8.4:73708038-73708057 None:intergenic 20.0%
!!! AAGTTAGTCATTAATTTTGT+AGG + chr8.4:73708037-73708056 MS.gene53329:intron 20.0%
!!! AGTTAGTCATTAATTTTGTA+GGG + chr8.4:73708038-73708057 MS.gene53329:intron 20.0%
! AAAATTAATGACTAACTTGG+TGG - chr8.4:73708035-73708054 None:intergenic 25.0%
! AAAGTAGATTATTAGAGATG+TGG + chr8.4:73707889-73707908 MS.gene53329:intron 25.0%
! AACTAGTATTAGAAAACTGT+TGG + chr8.4:73708505-73708524 MS.gene53329:intron 25.0%
! AATACTCATCAATATGCATT+AGG - chr8.4:73708558-73708577 None:intergenic 25.0%
! AATGATTCAAATGAGAAAAG+CGG - chr8.4:73707982-73708001 None:intergenic 25.0%
! ACAGATATATTCATTATGTC+AGG - chr8.4:73708080-73708099 None:intergenic 25.0%
! AGAATTCGTTCAAATTCAAA+AGG - chr8.4:73709246-73709265 None:intergenic 25.0%
! CAGATATATTCATTATGTCA+GGG - chr8.4:73708079-73708098 None:intergenic 25.0%
! TCATGTATATATATTCACCA+TGG - chr8.4:73709431-73709450 None:intergenic 25.0%
! TGATTACATCAAGTCTAAAT+AGG - chr8.4:73707952-73707971 None:intergenic 25.0%
! TGTTTATAGATTCTATTGGT+TGG + chr8.4:73709364-73709383 MS.gene53329:intron 25.0%
! TTGTGTCAAAAATATTTGCA+CGG + chr8.4:73709072-73709091 MS.gene53329:intron 25.0%
!! GAAGTGTTTATAGATTCTAT+TGG + chr8.4:73709360-73709379 MS.gene53329:intron 25.0%
!! GGAACTAAATTGATAATAAG+TGG + chr8.4:73708138-73708157 MS.gene53329:intron 25.0%
AGAAAACATGATATCCAAGA+AGG - chr8.4:73708369-73708388 None:intergenic 30.0%
AGAACAAACTCGTCAAAAAT+CGG + chr8.4:73709610-73709629 MS.gene53329:CDS 30.0%
ATTGAGTAGAAGAAGAATCT+TGG - chr8.4:73707738-73707757 None:intergenic 30.0%
GAAAACATGATATCCAAGAA+GGG - chr8.4:73708368-73708387 None:intergenic 30.0%
GTATATATGTGTTTGTGTGT+TGG + chr8.4:73708739-73708758 MS.gene53329:intron 30.0%
TACTCAAACGAACAACTATA+CGG - chr8.4:73709292-73709311 None:intergenic 30.0%
TATTGATGAGTATTTGCTCT+AGG + chr8.4:73708564-73708583 MS.gene53329:intron 30.0%
TTGTTATACTGGACAGAAAT+TGG - chr8.4:73708591-73708610 None:intergenic 30.0%
! AATATCATGTTTTCCTGCAA+AGG - chr8.4:73709208-73709227 None:intergenic 30.0%
! AGTTAGATTTTCTGACATAG+AGG - chr8.4:73707650-73707669 None:intergenic 30.0%
! ATTAGTGGTCAAACAGATTT+TGG - chr8.4:73708402-73708421 None:intergenic 30.0%
! CAAGTAGAAAAGTATAGAGA+TGG + chr8.4:73709877-73709896 MS.gene53329:CDS 30.0%
! CTTCTAAGAAGCTTAGTTAA+AGG - chr8.4:73709344-73709363 None:intergenic 30.0%
! GCTTAGTTAAAGGCATAAAT+AGG - chr8.4:73709334-73709353 None:intergenic 30.0%
! TTAGTGGTCAAACAGATTTT+GGG - chr8.4:73708401-73708420 None:intergenic 30.0%
!! AGTTCAGTGTTGATAAATAG+AGG - chr8.4:73708843-73708862 None:intergenic 30.0%
!! GTGCAAATATTTTTGACACA+AGG - chr8.4:73709073-73709092 None:intergenic 30.0%
!! TATTAGTCTTTGTGACAACA+TGG + chr8.4:73708113-73708132 MS.gene53329:intron 30.0%
!!! ACCAACTTGATGGTTTTATA+GGG + chr8.4:73708238-73708257 MS.gene53329:intron 30.0%
AAAAAGAGAAACACCTTTGC+AGG + chr8.4:73709192-73709211 MS.gene53329:intron 35.0%
AAACATTAAAGCCATGGTCT+TGG - chr8.4:73709804-73709823 None:intergenic 35.0%
AAATTAGTCCCTGACTTTGT+AGG - chr8.4:73708311-73708330 None:intergenic 35.0%
AAGCAGAGGAATTGTTATAC+TGG - chr8.4:73708602-73708621 None:intergenic 35.0%
AATTAGTCCCTGACTTTGTA+GGG - chr8.4:73708310-73708329 None:intergenic 35.0%
ACAAATCCTGCATATGAGAA+GGG - chr8.4:73709996-73710015 None:intergenic 35.0%
ACATTATTCCGTGATCCATA+TGG - chr8.4:73709023-73709042 None:intergenic 35.0%
ACCCTATAAAACCATCAAGT+TGG - chr8.4:73708242-73708261 None:intergenic 35.0%
AGAAGAGAGAGAAGTCTAAA+AGG - chr8.4:73708345-73708364 None:intergenic 35.0%
ATAGTGAGTGGTCAAATATG+GGG + chr8.4:73708215-73708234 MS.gene53329:intron 35.0%
CCTTGAAATTGAAAACCTAG+TGG - chr8.4:73707715-73707734 None:intergenic 35.0%
GATAGTGAGTGGTCAAATAT+GGG + chr8.4:73708214-73708233 MS.gene53329:intron 35.0%
GTGTTGTCAATTGTGAATTG+TGG + chr8.4:73708778-73708797 MS.gene53329:intron 35.0%
TACAAAGTCAGGGACTAATT+TGG + chr8.4:73708310-73708329 MS.gene53329:intron 35.0%
TCAAGTAACTCAATGGCATT+TGG - chr8.4:73709404-73709423 None:intergenic 35.0%
TCACAAAGATTAAGAGGCAA+AGG - chr8.4:73709513-73709532 None:intergenic 35.0%
TCTTCAGTGTTGTCAAATAG+TGG - chr8.4:73708910-73708929 None:intergenic 35.0%
TCTTCTTCTACTCAATCAAC+TGG + chr8.4:73707742-73707761 MS.gene53329:CDS 35.0%
TGCTTCGCATTCACATAAAT+TGG - chr8.4:73709540-73709559 None:intergenic 35.0%
TGGTACTTCAAGTAACTCAA+TGG - chr8.4:73709411-73709430 None:intergenic 35.0%
TTAATGTTTCCACCACTACT+CGG + chr8.4:73709815-73709834 MS.gene53329:CDS 35.0%
TTGACCACTCACTATCAATT+AGG - chr8.4:73708210-73708229 None:intergenic 35.0%
TTGTCAAATAGTGGCTATAG+CGG - chr8.4:73708901-73708920 None:intergenic 35.0%
TTTCTTATAGTCTGCCTTTC+AGG + chr8.4:73708944-73708963 MS.gene53329:intron 35.0%
TTTGCCTCTTAATCTTTGTG+AGG + chr8.4:73709512-73709531 MS.gene53329:CDS 35.0%
! ATCTTATCTGGAAGTTGGTT+CGG + chr8.4:73707771-73707790 MS.gene53329:CDS 35.0%
! CCACTAGGTTTTCAATTTCA+AGG + chr8.4:73707712-73707731 MS.gene53329:CDS 35.0%
! GGATATCATGTTTTCTACCA+TGG + chr8.4:73708373-73708392 MS.gene53329:intron 35.0%
! TGATAGTGAGTGGTCAAATA+TGG + chr8.4:73708213-73708232 MS.gene53329:intron 35.0%
! TTGAGTTACTTGAAGTACCA+TGG + chr8.4:73709411-73709430 MS.gene53329:intron 35.0%
!!! CTTGATGGTTTTATAGGGTT+AGG + chr8.4:73708243-73708262 MS.gene53329:intron 35.0%
!!! GACCAACTTGATGGTTTTAT+AGG + chr8.4:73708237-73708256 MS.gene53329:intron 35.0%
AAAGTCAGGGACTAATTTGG+CGG + chr8.4:73708313-73708332 MS.gene53329:intron 40.0%
AAATATGGGGACCAACTTGA+TGG + chr8.4:73708228-73708247 MS.gene53329:intron 40.0%
AACCTAGTGGTTTAGACTTG+TGG - chr8.4:73707702-73707721 None:intergenic 40.0%
AACTGGTCGTCAATCTTATC+TGG + chr8.4:73707759-73707778 MS.gene53329:CDS 40.0%
AATATACTCCCCTCACTTAC+AGG - chr8.4:73708432-73708451 None:intergenic 40.0%
AATGTCTAGTCCTGTAAGTG+AGG + chr8.4:73708419-73708438 MS.gene53329:intron 40.0%
AGCAGTTTCTTCCAATGCTA+TGG + chr8.4:73708806-73708825 MS.gene53329:intron 40.0%
AGTCTTTGTGACAACATGGT+AGG + chr8.4:73708117-73708136 MS.gene53329:intron 40.0%
ATGTCTAGTCCTGTAAGTGA+GGG + chr8.4:73708420-73708439 MS.gene53329:intron 40.0%
ATTTGATGCCCTACAAAGTC+AGG + chr8.4:73708299-73708318 MS.gene53329:intron 40.0%
CATGATACACAAATCTTGCC+AGG + chr8.4:73709446-73709465 MS.gene53329:intron 40.0%
CATGCTGAGACATACTCTAA+TGG + chr8.4:73708984-73709003 MS.gene53329:CDS 40.0%
CGTCAATCTTATCTGGAAGT+TGG + chr8.4:73707766-73707785 MS.gene53329:CDS 40.0%
CTTACAGGACTAGACATTAG+TGG - chr8.4:73708417-73708436 None:intergenic 40.0%
GAATGCAACATGATCCTGAA+AGG - chr8.4:73708961-73708980 None:intergenic 40.0%
GACAAATCCTGCATATGAGA+AGG - chr8.4:73709997-73710016 None:intergenic 40.0%
GGTTCCTCACAAAGATTAAG+AGG - chr8.4:73709519-73709538 None:intergenic 40.0%
GTCTACGTCTCATAATAGCA+TGG - chr8.4:73709566-73709585 None:intergenic 40.0%
TAGAGTATGTCTCAGCATGA+GGG - chr8.4:73708983-73709002 None:intergenic 40.0%
TGAAGTCGGATTCATGTTGT+TGG - chr8.4:73709960-73709979 None:intergenic 40.0%
TGATTGTGATCCAGTTGAGA+AGG - chr8.4:73708708-73708727 None:intergenic 40.0%
TGGAAATGTGCTGGAATCAA+GGG + chr8.4:73709849-73709868 MS.gene53329:CDS 40.0%
TGGTGGAAACATTAAAGCCA+TGG - chr8.4:73709810-73709829 None:intergenic 40.0%
TGTTCAACACTTAGCGAAAC+CGG + chr8.4:73708621-73708640 MS.gene53329:CDS 40.0%
TTAGAGTATGTCTCAGCATG+AGG - chr8.4:73708984-73709003 None:intergenic 40.0%
TTTGATGCCCTACAAAGTCA+GGG + chr8.4:73708300-73708319 MS.gene53329:intron 40.0%
! GACTTTGTAGGGCATCAAAT+AGG - chr8.4:73708299-73708318 None:intergenic 40.0%
! GTAACCACATGATCTGAAGT+CGG - chr8.4:73709974-73709993 None:intergenic 40.0%
!! AAGAGTGTGGAAGGTATCAT+CGG + chr8.4:73708648-73708667 MS.gene53329:CDS 40.0%
!! ATTAGAAGCACTTGTGACGT+CGG - chr8.4:73709923-73709942 None:intergenic 40.0%
!! CAAACAGATTTTGGGTACCA+TGG - chr8.4:73708393-73708412 None:intergenic 40.0%
!! GAGACCTAATTGATAGTGAG+TGG + chr8.4:73708203-73708222 MS.gene53329:intron 40.0%
AAGAGGCAAAGGAATCCGAA+CGG - chr8.4:73709502-73709521 None:intergenic 45.0%
ACCAAAAAGCTCCAAGACCA+TGG + chr8.4:73709790-73709809 MS.gene53329:CDS 45.0%
ATCCATATGGAGCTGCTAGT+AGG - chr8.4:73709010-73709029 None:intergenic 45.0%
ATGAAACACTCATGGCCATG+GGG + chr8.4:73707595-73707614 MS.gene53329:CDS 45.0%
CATGCATTGAAGAGAGCTAG+AGG + chr8.4:73709745-73709764 MS.gene53329:CDS 45.0%
CGTTTGAGTAAATGCTGCAG+TGG + chr8.4:73709302-73709321 MS.gene53329:intron 45.0%
CTAGCTGAGTATGATGAACC+TGG - chr8.4:73709467-73709486 None:intergenic 45.0%
CTATAGCGACACCATAGCAT+TGG - chr8.4:73708820-73708839 None:intergenic 45.0%
CTCTCTTCAATGCATGCAGA+TGG - chr8.4:73709741-73709760 None:intergenic 45.0%
GAATCCGACTTCAGATCATG+TGG + chr8.4:73709967-73709986 MS.gene53329:CDS 45.0%
GATTCCAGCACATTTCCACT+CGG - chr8.4:73709847-73709866 None:intergenic 45.0%
GTATCATCGGATCATCTGCA+GGG + chr8.4:73708661-73708680 MS.gene53329:CDS 45.0%
GTGGAAATGTGCTGGAATCA+AGG + chr8.4:73709848-73709867 MS.gene53329:CDS 45.0%
GTTCATCATACTCAGCTAGC+AGG + chr8.4:73709468-73709487 MS.gene53329:CDS 45.0%
TAGCAGCTCCATATGGATCA+CGG + chr8.4:73709012-73709031 MS.gene53329:CDS 45.0%
TGCCACAAGTCTAAACCACT+AGG + chr8.4:73707697-73707716 MS.gene53329:CDS 45.0%
TGGTTACCCTTCTCATATGC+AGG + chr8.4:73709987-73710006 MS.gene53329:CDS 45.0%
TGTCTAGTCCTGTAAGTGAG+GGG + chr8.4:73708421-73708440 MS.gene53329:intron 45.0%
TTCTCTCTCTTCTCCCTTCT+TGG + chr8.4:73708352-73708371 MS.gene53329:intron 45.0%
TTGTGATCCAGTTGAGAAGG+AGG - chr8.4:73708705-73708724 None:intergenic 45.0%
!! GAAGTTGGTTCGGAACAATC+CGG + chr8.4:73707781-73707800 MS.gene53329:CDS 45.0%
!! GCTAAGTGTTGAACAAGCAG+AGG - chr8.4:73708616-73708635 None:intergenic 45.0%
ACTTGTGACGTCGGAACAAG+TGG - chr8.4:73709914-73709933 None:intergenic 50.0%
AGAGGCAAAGGAATCCGAAC+GGG - chr8.4:73709501-73709520 None:intergenic 50.0%
ATCCAGTTGAGAAGGAGGCA+AGG - chr8.4:73708700-73708719 None:intergenic 50.0%
GAATCCGAGTGGAAATGTGC+TGG + chr8.4:73709840-73709859 MS.gene53329:CDS 50.0%
GATTCAGAACAAGTCCCCCA+TGG - chr8.4:73707613-73707632 None:intergenic 50.0%
GGTATCATCGGATCATCTGC+AGG + chr8.4:73708660-73708679 MS.gene53329:CDS 50.0%
TACCTTGCCTCCTTCTCAAC+TGG + chr8.4:73708695-73708714 MS.gene53329:CDS 50.0%
TCAACACTTAGCGAAACCGG+CGG + chr8.4:73708624-73708643 MS.gene53329:CDS 50.0%
TGAAACACTCATGGCCATGG+GGG + chr8.4:73707596-73707615 MS.gene53329:CDS 50.0%
TTGAAGAGAGCTAGAGGCTC+TGG + chr8.4:73709751-73709770 MS.gene53329:CDS 50.0%
!! GCCATGGTCTTGGAGCTTTT+TGG - chr8.4:73709794-73709813 None:intergenic 50.0%
ACCTTCCACACTCTTTCCGC+CGG - chr8.4:73708643-73708662 None:intergenic 55.0%
ACTACTCGGCTGAATCCGAG+TGG + chr8.4:73709829-73709848 MS.gene53329:CDS 55.0%
ACTCGGATTCAGCCGAGTAG+TGG - chr8.4:73709830-73709849 None:intergenic 55.0%
CAGGATTTGTCAGCCGATGC+CGG + chr8.4:73710006-73710025 MS.gene53329:CDS 55.0%
GACGTCGGAACAAGTGGTTG+TGG - chr8.4:73709908-73709927 None:intergenic 55.0%
GGCCTACTAGCAGCTCCATA+TGG + chr8.4:73709005-73709024 MS.gene53329:CDS 55.0%
TAGCAGGAATGCCTCCCGTT+CGG + chr8.4:73709484-73709503 MS.gene53329:CDS 55.0%
TGGCGCGACTCGTGTAGATA+TGG - chr8.4:73709721-73709740 None:intergenic 55.0%
! AAGAGAGCTAGAGGCTCTGG+TGG + chr8.4:73709754-73709773 MS.gene53329:CDS 55.0%
ATTCCGACCACCACCACCAC+CGG - chr8.4:73710028-73710047 None:intergenic 60.0%
CGAAACCGGCGGAAAGAGTG+TGG + chr8.4:73708635-73708654 MS.gene53329:CDS 60.0%
CGGATCATCTGCAGGGAGTC+AGG + chr8.4:73708668-73708687 MS.gene53329:CDS 60.0%
CGGATTCAGCCGAGTAGTGG+TGG - chr8.4:73709827-73709846 None:intergenic 60.0%
GATTTGTCAGCCGATGCCGG+TGG + chr8.4:73710009-73710028 MS.gene53329:CDS 60.0%
GGCAAAGGAATCCGAACGGG+AGG - chr8.4:73709498-73709517 None:intergenic 60.0%
! ACCGGCGGAAAGAGTGTGGA+AGG + chr8.4:73708639-73708658 MS.gene53329:CDS 60.0%
ACCACCACCACCACCGGCAT+CGG - chr8.4:73710022-73710041 None:intergenic 65.0%
TTGTCAGCCGATGCCGGTGG+TGG + chr8.4:73710012-73710031 MS.gene53329:CDS 65.0%
!! ATGCCGGTGGTGGTGGTGGT+CGG + chr8.4:73710022-73710041 MS.gene53329:CDS 65.0%
! TCAGCCGATGCCGGTGGTGG+TGG + chr8.4:73710015-73710034 MS.gene53329:CDS 70.0%
!! GCCGATGCCGGTGGTGGTGG+TGG + chr8.4:73710018-73710037 MS.gene53329:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 73707595 73710074 73707595 ID=MS.gene53329
chr8.4 mRNA 73707595 73710074 73707595 ID=MS.gene53329.t1;Parent=MS.gene53329
chr8.4 exon 73707595 73707802 73707595 ID=MS.gene53329.t1.exon1;Parent=MS.gene53329.t1
chr8.4 CDS 73707595 73707802 73707595 ID=cds.MS.gene53329.t1;Parent=MS.gene53329.t1
chr8.4 exon 73708586 73708734 73708586 ID=MS.gene53329.t1.exon2;Parent=MS.gene53329.t1
chr8.4 CDS 73708586 73708734 73708586 ID=cds.MS.gene53329.t1;Parent=MS.gene53329.t1
chr8.4 exon 73708966 73709040 73708966 ID=MS.gene53329.t1.exon3;Parent=MS.gene53329.t1
chr8.4 CDS 73708966 73709040 73708966 ID=cds.MS.gene53329.t1;Parent=MS.gene53329.t1
chr8.4 exon 73709468 73709635 73709468 ID=MS.gene53329.t1.exon4;Parent=MS.gene53329.t1
chr8.4 CDS 73709468 73709635 73709468 ID=cds.MS.gene53329.t1;Parent=MS.gene53329.t1
chr8.4 exon 73709718 73710074 73709718 ID=MS.gene53329.t1.exon5;Parent=MS.gene53329.t1
chr8.4 CDS 73709718 73710074 73709718 ID=cds.MS.gene53329.t1;Parent=MS.gene53329.t1
Gene Sequence

>MS.gene53329

ATGAAACACTCATGGCCATGGGGGACTTGTTCTGAATCTGATGTGCAACAATCCTCTATGTCAGAAAATCTAACTTTGAATATGAGTGTTTTGCAACAAGAATGCCACAAGTCTAAACCACTAGGTTTTCAATTTCAAGGCCAAGATTCTTCTTCTACTCAATCAACTGGTCGTCAATCTTATCTGGAAGTTGGTTCGGAACAATCCGGTAACTATATATTCGTAATTAAATCTGTTACATATCTGCATTTGACTCTTGTTATCAATTGTTTTTTGTTATTTCTATAAGCATCAAAAGTAGATTATTAGAGATGTGGTTAGTAGTTGATTATTATTTAGACTACTTTGTGAATGCCTATTTAGACTTGATGTAATCATTTGAATCCGCTTTTCTCATTTGAATCATTTTACACTCATCAATACAGTAAACAAGTAAACCACCAAGTTAGTCATTAATTTTGTAGGGCGCTCTCATATAAATCCCTGACATAATGAATATATCTGTAAAACTTTACTCATATTAGTCTTTGTGACAACATGGTAGGAACTAAATTGATAATAAGTGGTTAAATATAAGGACCAAATTGATAGTTAATTGTTAAGATATAGAGACCTAATTGATAGTGAGTGGTCAAATATGGGGACCAACTTGATGGTTTTATAGGGTTAGGACCTTTTTAAAATATTCATAATGTCAGAGACCTATTTGATGCCCTACAAAGTCAGGGACTAATTTGGCGGTTTACTCCTTTTAGACTTCTCTCTCTTCTCCCTTCTTGGATATCATGTTTTCTACCATGGTACCCAAAATCTGTTTGACCACTAATGTCTAGTCCTGTAAGTGAGGGGAGTATATTAAGAGTCACACATTGTTCTTTCCAAAGATTGAAATTAATATTTTAGACAAATCAACTAGTATTAGAAAACTGTTGGAGTACAAAATTTAAACTTTTGTCACTTCCTAATGCATATTGATGAGTATTTGCTCTAGGTCCAATTTCTGTCCAGTATAACAATTCCTCTGCTTGTTCAACACTTAGCGAAACCGGCGGAAAGAGTGTGGAAGGTATCATCGGATCATCTGCAGGGAGTCAGGATTTTACCTTGCCTCCTTCTCAACTGGATCACAATCAATCACTTGTAAGTATATATGTGTTTGTGTGTTGGTGCAAAGAAGATATAAGTGTTGTCAATTGTGAATTGTGGAAAATAGCAGTTTCTTCCAATGCTATGGTGTCGCTATAGCCTCTATTTATCAACACTGAACTAAATAGCGTATCACAAATTAATAGCAATTGTAGCGCCGCTATAGCCACTATTTGACAACACTGAAGATAGTTGCAATTTTTTTTCTTATAGTCTGCCTTTCAGGATCATGTTGCATTCCCTCATGCTGAGACATACTCTAATGGCCTACTAGCAGCTCCATATGGATCACGGAATAATGTAAGATTTGAGTTTGTTCATTTCTTTTACCTTGTGTCAAAAATATTTGCACGGAAAATTATGCCATCATATTTATGTCTTTATCTGGAATGTACAATGTTGCTTTTGCTTCTGATTTTGCAGTGTAGTCTTTGTTATGTCACGAAATTAGAAAAAGAGAAACACCTTTGCAGGAAAACATGATATTCTTATTGTAACTTTTCCTTTTGAATTTGAACGAATTCTGTTCTAAGTTTTTTGTGATTCTTCCGTATAGTTGTTCGTTTGAGTAAATGCTGCAGTGGTTGAATCCTATTTATGCCTTTAACTAAGCTTCTTAGAAGTGTTTATAGATTCTATTGGTTGGTTTTCAATTTATATCCAAATGCCATTGAGTTACTTGAAGTACCATGGTGAATATATATACATGATACACAAATCTTGCCAGGTTCATCATACTCAGCTAGCAGGAATGCCTCCCGTTCGGATTCCTTTGCCTCTTAATCTTTGTGAGGAACCAATTTATGTGAATGCGAAGCAGTACCATGCTATTATGAGACGTAGACAGTATCGTGCAAAGCTCGAAGCACAGAACAAACTCGTCAAAAATCGGAAAGTAAGCATACTTTTTTGTTCTTTTTCTCTAATGCAAATTTCTCTTTATTTCTCTCAATAACATTTTGAATATTGTCTTGCAGCCATATCTACACGAGTCGCGCCATCTGCATGCATTGAAGAGAGCTAGAGGCTCTGGTGGACGTTTTCTAAATACCAAAAAGCTCCAAGACCATGGCTTTAATGTTTCCACCACTACTCGGCTGAATCCGAGTGGAAATGTGCTGGAATCAAGGGTACATCAAGTAGAAAAGTATAGAGATGGTGCTTCCACAACCACTTGTTCCGACGTCACAAGTGCTTCTAATAGTGACGACATGTTCCAACAACATGAATCCGACTTCAGATCATGTGGTTACCCTTCTCATATGCAGGATTTGTCAGCCGATGCCGGTGGTGGTGGTGGTCGGAATCAACACCGCCTATCTGTCCTTATGTGA
Protein sequence

>MS.gene53329.t1

MKHSWPWGTCSESDVQQSSMSENLTLNMSVLQQECHKSKPLGFQFQGQDSSSTQSTGRQSYLEVGSEQSGPISVQYNNSSACSTLSETGGKSVEGIIGSSAGSQDFTLPPSQLDHNQSLDHVAFPHAETYSNGLLAAPYGSRNNVHHTQLAGMPPVRIPLPLNLCEEPIYVNAKQYHAIMRRRQYRAKLEAQNKLVKNRKPYLHESRHLHALKRARGSGGRFLNTKKLQDHGFNVSTTTRLNPSGNVLESRVHQVEKYRDGASTTTCSDVTSASNSDDMFQQHESDFRSCGYPSHMQDLSADAGGGGGRNQHRLSVLM