AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53385

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53385.t1 MTR_4g073220 89.764 127 11 1 1 127 484 608 1.73e-77 251
MS.gene53385.t1 MTR_7g075000 61.582 177 66 1 142 318 43 217 5.72e-58 194
MS.gene53385.t1 MTR_1g026990 53.571 140 55 4 1 132 171 308 1.83e-39 142
MS.gene53385.t1 MTR_1g027160 50.968 155 66 4 1 147 171 323 3.71e-39 141
MS.gene53385.t1 MTR_1g028280 58.475 118 44 3 1 115 542 657 7.85e-38 143
MS.gene53385.t1 MTR_1g028280 58.475 118 44 3 1 115 503 618 9.42e-38 142
MS.gene53385.t1 MTR_1g027250 52.593 135 58 3 1 129 171 305 1.99e-37 136
MS.gene53385.t1 MTR_1g027460 56.780 118 46 3 1 115 198 313 8.87e-37 135
MS.gene53385.t1 MTR_1g027540 52.174 138 56 4 1 130 273 408 9.52e-36 134
MS.gene53385.t1 MTR_1g027680 54.622 119 48 3 1 115 561 677 1.82e-33 130
MS.gene53385.t1 MTR_1g027990 53.782 119 49 3 1 115 564 680 1.98e-33 130
MS.gene53385.t1 MTR_1g027320 52.893 121 50 3 1 114 171 291 5.47e-33 124
MS.gene53385.t1 MTR_1g031530 51.724 116 53 2 1 113 199 314 1.29e-32 124
MS.gene53385.t1 MTR_1g027730 53.390 118 50 3 1 115 197 312 1.32e-32 124
MS.gene53385.t1 MTR_1g031430 50.000 116 55 2 1 113 51 166 5.39e-32 118
MS.gene53385.t1 MTR_6g045030 44.371 151 73 5 1 148 277 419 1.30e-31 122
MS.gene53385.t1 MTR_3g028630 50.847 118 53 3 1 115 268 383 2.03e-31 122
MS.gene53385.t1 MTR_1g027840 52.542 118 51 3 1 115 197 312 2.58e-31 120
MS.gene53385.t1 MTR_1g027830 52.542 118 51 3 1 115 524 639 4.24e-31 123
MS.gene53385.t1 MTR_1g027930 51.695 118 52 3 1 115 141 256 4.87e-31 119
MS.gene53385.t1 MTR_1g027410 55.085 118 48 3 1 115 270 385 1.98e-30 119
MS.gene53385.t1 MTR_1g031510 52.586 116 52 2 1 113 486 601 1.98e-30 121
MS.gene53385.t1 MTR_1g028100 50.420 119 53 4 1 115 258 374 3.91e-30 118
MS.gene53385.t1 MTR_1g031280 49.138 116 56 2 1 113 515 630 2.50e-29 118
MS.gene53385.t1 MTR_1g028020 43.949 157 64 6 1 148 316 457 2.95e-29 118
MS.gene53385.t1 MTR_1g028170 42.767 159 77 5 1 155 285 433 7.01e-29 115
MS.gene53385.t1 MTR_1g027440 51.304 115 44 3 1 115 449 551 1.06e-28 116
MS.gene53385.t1 MTR_1g031200 49.565 115 55 2 2 113 370 484 1.13e-28 115
MS.gene53385.t1 MTR_1g027140 51.261 119 54 3 1 115 601 719 2.50e-28 115
MS.gene53385.t1 MTR_1g027050 51.261 119 54 3 1 115 604 722 3.32e-28 115
MS.gene53385.t1 MTR_1g031520 50.427 117 55 2 1 114 483 599 1.47e-27 113
MS.gene53385.t1 MTR_1g027070 50.000 120 52 4 1 115 567 683 2.43e-27 112
MS.gene53385.t1 MTR_1g031560 48.718 117 57 2 1 114 504 620 2.33e-26 109
MS.gene53385.t1 MTR_1g027030 49.580 119 54 3 1 115 603 719 5.51e-26 108
MS.gene53385.t1 MTR_1g027030 48.387 124 58 3 1 120 553 674 5.72e-26 108
MS.gene53385.t1 MTR_1g028290 44.915 118 60 3 1 115 458 573 2.42e-25 106
MS.gene53385.t1 MTR_6g082830 43.220 118 62 2 1 115 173 288 8.29e-25 102
MS.gene53385.t1 MTR_6g082860 43.220 118 62 2 1 115 229 344 3.71e-24 101
MS.gene53385.t1 MTR_1g110220 43.802 121 58 4 1 115 423 539 6.67e-24 102
MS.gene53385.t1 MTR_1g110140 40.288 139 72 5 1 133 225 358 1.65e-23 99.8
MS.gene53385.t1 MTR_7g085860 40.678 118 65 3 1 115 334 449 6.25e-23 99.0
MS.gene53385.t1 MTR_1g110260 40.496 121 62 4 1 115 403 519 2.06e-22 98.2
MS.gene53385.t1 MTR_1g115485 40.336 119 65 3 1 115 530 646 2.29e-22 98.2
MS.gene53385.t1 MTR_1g031580 45.000 120 57 4 1 115 470 585 6.00e-22 96.7
MS.gene53385.t1 MTR_6g083210 41.322 121 61 5 1 115 173 289 1.05e-21 93.6
MS.gene53385.t1 MTR_6g083290 41.322 121 61 5 1 115 185 301 2.02e-21 93.2
MS.gene53385.t1 MTR_7g082400 38.333 120 67 3 1 115 253 370 2.62e-21 94.0
MS.gene53385.t1 MTR_6g083780 40.678 118 65 3 1 115 522 637 3.06e-21 94.7
MS.gene53385.t1 MTR_7g082280 38.333 120 67 3 1 115 596 713 3.71e-21 94.7
MS.gene53385.t1 MTR_4g073230 40.678 118 65 3 1 115 468 583 4.29e-21 94.4
MS.gene53385.t1 MTR_6g083270 41.322 121 61 5 1 115 328 444 4.77e-21 93.6
MS.gene53385.t1 MTR_1g027970 38.889 126 73 3 1 124 459 582 4.84e-21 94.0
MS.gene53385.t1 MTR_6g082950 38.710 124 60 4 1 115 293 409 5.34e-21 93.2
MS.gene53385.t1 MTR_1g031460 42.500 120 60 4 1 115 461 576 5.50e-21 94.0
MS.gene53385.t1 MTR_1g031320 43.333 120 59 4 1 115 474 589 6.73e-21 93.6
MS.gene53385.t1 MTR_7g082300 34.899 149 84 5 1 141 719 862 7.28e-21 94.0
MS.gene53385.t1 MTR_1g027420 40.517 116 67 2 1 115 416 530 1.00e-20 93.2
MS.gene53385.t1 MTR_7g082510 37.815 119 68 3 1 115 515 631 1.10e-20 93.2
MS.gene53385.t1 MTR_1g027500 39.316 117 67 3 1 115 473 587 1.41e-20 92.8
MS.gene53385.t1 MTR_6g082870 39.496 119 66 4 1 115 530 646 1.73e-20 92.4
MS.gene53385.t1 MTR_1g026940 35.915 142 83 3 1 136 472 611 1.80e-20 92.4
MS.gene53385.t1 MTR_1g031540 42.373 118 63 3 1 115 165 280 2.02e-20 92.4
MS.gene53385.t1 MTR_1g031540 40.708 113 40 1 1 113 518 603 1.96e-18 86.7
MS.gene53385.t1 MTR_6g083300 38.710 124 60 4 1 115 173 289 2.03e-20 90.1
MS.gene53385.t1 MTR_1g031540 42.373 118 63 3 1 115 457 572 2.04e-20 92.4
MS.gene53385.t1 MTR_1g031540 40.708 113 40 1 1 113 810 895 1.60e-18 87.0
MS.gene53385.t1 MTR_7g082490 37.815 119 68 3 1 115 280 396 2.52e-20 91.3
MS.gene53385.t1 MTR_7g082110 38.333 120 66 4 1 115 705 821 2.55e-20 92.0
MS.gene53385.t1 MTR_1g027690 38.095 126 74 3 1 124 457 580 4.67e-20 91.3
MS.gene53385.t1 MTR_7g082380 37.984 129 72 4 1 124 230 355 5.30e-20 89.7
MS.gene53385.t1 MTR_7g082470 40.000 120 65 4 1 115 503 620 5.98e-20 90.9
MS.gene53385.t1 MTR_4g044393 39.167 120 65 4 1 115 703 819 6.46e-20 90.9
MS.gene53385.t1 MTR_6g083760 40.336 119 65 4 1 115 493 609 6.58e-20 90.9
MS.gene53385.t1 MTR_8g104520 40.164 122 64 4 1 115 513 632 8.40e-20 90.5
MS.gene53385.t1 MTR_1g028080 39.316 117 68 3 1 115 459 574 1.64e-19 89.7
MS.gene53385.t1 MTR_7g082430 38.655 119 67 3 1 115 484 600 1.75e-19 89.4
MS.gene53385.t1 MTR_1g108910 40.870 115 53 3 1 115 211 310 3.26e-19 87.0
MS.gene53385.t1 MTR_1g110270 39.831 118 61 3 1 115 491 601 3.71e-19 88.6
MS.gene53385.t1 MTR_1g110230 40.678 118 60 4 4 115 472 585 4.75e-19 88.2
MS.gene53385.t1 MTR_1g028130 39.316 117 68 3 1 115 459 574 4.96e-19 88.2
MS.gene53385.t1 MTR_0394s0020 37.500 120 67 4 1 115 243 359 5.66e-19 87.0
MS.gene53385.t1 MTR_7g082360 38.655 119 65 4 1 115 207 321 6.00e-19 86.7
MS.gene53385.t1 MTR_1g027370 39.496 119 65 4 1 115 473 588 3.71e-18 85.5
MS.gene53385.t1 MTR_1g028220 41.525 118 64 4 1 115 430 545 5.14e-18 85.1
MS.gene53385.t1 MTR_7g082460 36.975 119 69 3 1 115 519 635 7.77e-18 84.7
MS.gene53385.t1 MTR_2g036460 39.091 110 52 3 13 112 322 426 1.62e-17 83.2
MS.gene53385.t1 MTR_1g027200 39.316 117 59 3 1 115 402 508 2.42e-17 82.8
MS.gene53385.t1 MTR_6g083740 37.615 109 62 3 11 115 122 228 3.03e-17 80.1
MS.gene53385.t1 MTR_2g036500 38.393 112 61 3 2 107 244 353 3.37e-17 82.0
MS.gene53385.t1 MTR_1g027820 38.525 122 63 4 1 115 426 542 3.39e-17 82.4
MS.gene53385.t1 MTR_1g027060 37.815 119 67 4 1 115 375 490 3.61e-17 82.4
MS.gene53385.t1 MTR_0690s0010 36.220 127 75 3 1 124 407 530 7.52e-17 81.6
MS.gene53385.t1 MTR_1g027890 36.752 117 70 2 1 115 272 386 1.25e-16 80.5
MS.gene53385.t1 MTR_6g445600 36.220 127 75 3 1 124 433 556 1.30e-16 80.9
MS.gene53385.t1 MTR_1g027150 36.975 119 68 4 1 115 428 543 2.57e-16 80.1
MS.gene53385.t1 MTR_2g036430 38.679 106 58 4 2 102 228 331 2.75e-16 79.3
MS.gene53385.t1 MTR_7g060570 34.746 118 70 3 1 115 509 622 2.90e-16 80.1
MS.gene53385.t1 MTR_2g036440 38.393 112 61 3 2 107 245 354 5.51e-16 78.6
MS.gene53385.t1 MTR_1g027960 37.815 119 67 4 1 115 447 562 1.04e-15 78.2
MS.gene53385.t1 MTR_1g027040 36.667 120 68 4 1 115 430 546 1.08e-15 78.2
MS.gene53385.t1 MTR_2g036490 35.238 105 63 1 13 112 312 416 2.57e-15 76.6
MS.gene53385.t1 MTR_1g031400 60.345 58 22 1 1 57 189 246 3.28e-15 74.7
MS.gene53385.t1 MTR_3g028650 37.288 118 69 3 1 115 428 543 4.26e-15 76.3
MS.gene53385.t1 MTR_2g054870 39.683 126 51 7 1 106 278 398 1.93e-14 73.9
MS.gene53385.t1 MTR_1g027700 36.441 118 70 3 1 115 203 318 3.81e-14 72.8
MS.gene53385.t1 MTR_1g110160 37.288 118 49 5 4 115 466 564 4.41e-14 73.2
MS.gene53385.t1 MTR_8g058250 34.146 164 93 5 1 154 833 991 5.02e-14 73.2
MS.gene53385.t1 MTR_8g058250 34.146 164 93 5 1 154 737 895 5.05e-14 73.2
MS.gene53385.t1 MTR_4g113710 38.136 118 52 6 1 102 276 388 6.39e-14 72.4
MS.gene53385.t1 MTR_8g465150 34.188 117 71 2 1 113 686 800 9.06e-14 72.4
MS.gene53385.t1 MTR_1g031470 42.529 87 48 1 29 113 1 87 2.15e-13 66.2
MS.gene53385.t1 MTR_6g012810 35.833 120 68 4 1 115 770 885 6.34e-13 70.1
MS.gene53385.t1 MTR_4g091570 36.364 132 74 5 1 128 924 1049 2.90e-12 68.2
MS.gene53385.t1 MTR_8g465160 33.333 117 72 2 1 113 688 802 3.93e-12 67.4
MS.gene53385.t1 MTR_8g028065 35.156 128 72 5 1 122 740 862 7.10e-12 66.6
MS.gene53385.t1 MTR_4g081685 35.833 120 69 3 1 115 674 790 7.24e-12 66.6
MS.gene53385.t1 MTR_6g043510 30.556 144 83 7 1 133 658 795 7.68e-12 66.6
MS.gene53385.t1 MTR_2g073650 37.273 110 62 2 1 105 831 938 1.07e-11 66.2
MS.gene53385.t1 MTR_6g463710 33.083 133 71 4 1 123 743 867 1.10e-11 66.2
MS.gene53385.t1 MTR_6g057770 33.083 133 71 4 1 123 743 867 1.14e-11 66.2
MS.gene53385.t1 MTR_0007s0020 33.577 137 72 5 1 127 743 870 1.15e-11 66.2
MS.gene53385.t1 MTR_3g107070 34.043 141 75 4 1 136 641 768 1.39e-11 65.9
MS.gene53385.t1 MTR_8g035560 34.375 128 70 6 1 117 550 674 1.49e-11 65.5
MS.gene53385.t1 MTR_6g463700 32.031 128 76 3 1 123 712 833 1.66e-11 65.5
MS.gene53385.t1 MTR_8g465590 33.333 120 70 3 1 115 8 122 1.86e-11 61.6
MS.gene53385.t1 MTR_6g057750 32.031 128 76 3 1 123 757 878 1.86e-11 65.5
MS.gene53385.t1 MTR_8g016120 36.364 110 63 2 1 105 573 680 1.97e-11 65.5
MS.gene53385.t1 MTR_3g011910 33.088 136 74 5 1 127 496 623 3.10e-11 64.7
MS.gene53385.t1 MTR_1g012550 36.667 120 65 3 1 111 669 786 3.19e-11 64.7
MS.gene53385.t1 MTR_2g074980 37.069 116 65 3 1 110 799 912 3.29e-11 64.7
MS.gene53385.t1 MTR_6g053000 32.609 138 74 5 1 128 508 636 3.35e-11 64.7
MS.gene53385.t1 MTR_0074s0060 32.609 138 74 5 1 128 479 607 3.53e-11 64.3
MS.gene53385.t1 MTR_2g011150 32.117 137 75 5 1 128 630 757 3.54e-11 64.7
MS.gene53385.t1 MTR_2g075060 37.273 110 62 2 1 105 831 938 3.57e-11 64.7
MS.gene53385.t1 MTR_2g075250 34.483 116 68 3 1 111 835 947 4.32e-11 64.3
MS.gene53385.t1 MTR_2g075250 34.483 116 68 3 1 111 740 852 4.40e-11 64.3
MS.gene53385.t1 MTR_4g114280 35.200 125 71 4 1 119 720 840 5.28e-11 63.9
MS.gene53385.t1 MTR_2g074990 37.273 110 62 2 1 105 821 928 5.50e-11 63.9
MS.gene53385.t1 MTR_8g061110 28.767 146 95 3 1 139 576 719 5.85e-11 63.9
MS.gene53385.t1 MTR_2g074820 37.273 110 62 2 1 105 305 412 8.93e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53385.t1 AT5G38280 43.571 140 67 5 1 132 499 634 5.04e-25 105
MS.gene53385.t1 AT5G38280 43.571 140 67 5 1 132 499 634 6.68e-25 105
MS.gene53385.t1 AT5G38250 43.902 123 58 5 1 115 475 594 2.34e-23 100
MS.gene53385.t1 AT5G38240 41.463 123 61 4 1 115 478 597 2.93e-23 100
MS.gene53385.t1 AT5G38240 41.463 123 61 4 1 115 432 551 3.42e-23 100
MS.gene53385.t1 AT5G38240 41.463 123 61 4 1 115 496 615 3.51e-23 100
MS.gene53385.t1 AT1G67000 39.695 131 61 4 1 115 724 852 1.76e-22 98.6
MS.gene53385.t1 AT5G39030 43.089 123 59 4 1 115 665 784 1.55e-21 95.9
MS.gene53385.t1 AT5G39030 43.089 123 59 4 1 115 659 778 1.86e-21 95.5
MS.gene53385.t1 AT1G66930 39.370 127 60 4 1 115 514 635 5.40e-20 91.3
MS.gene53385.t1 AT1G66930 39.370 127 60 4 1 115 483 604 6.64e-20 90.9
MS.gene53385.t1 AT4G18250 40.000 125 63 5 1 115 524 646 8.00e-20 90.5
MS.gene53385.t1 AT1G66930 39.370 127 60 4 1 115 723 844 8.41e-20 90.9
MS.gene53385.t1 AT5G39020 37.778 135 77 3 1 130 663 795 1.72e-19 89.7
MS.gene53385.t1 AT5G38260 40.650 123 62 4 1 115 488 607 2.15e-19 89.4
MS.gene53385.t1 AT1G70250 37.903 124 65 4 1 115 627 747 3.65e-19 89.0
MS.gene53385.t1 AT1G66980 36.585 123 68 3 1 115 955 1075 8.05e-19 87.8
MS.gene53385.t1 AT1G66980 36.585 123 68 3 1 115 1001 1121 8.60e-19 87.8
MS.gene53385.t1 AT1G66980 36.585 123 68 3 1 115 972 1092 8.70e-19 87.8
MS.gene53385.t1 AT1G66980 36.585 123 68 3 1 115 999 1119 9.00e-19 87.8
MS.gene53385.t1 AT1G66910 37.143 140 69 5 1 125 512 647 1.07e-17 84.3
MS.gene53385.t1 AT1G66910 37.143 140 69 5 1 125 515 650 1.33e-17 84.0
MS.gene53385.t1 AT1G66920 39.516 124 60 5 1 115 475 592 1.36e-17 84.0
MS.gene53385.t1 AT1G66920 39.516 124 60 5 1 115 449 566 1.37e-17 84.0
MS.gene53385.t1 AT1G66920 39.516 124 60 5 1 115 467 584 1.43e-17 84.0
MS.gene53385.t1 AT4G23320 35.294 119 69 2 1 113 373 489 1.64e-14 74.3
MS.gene53385.t1 AT4G23320 35.294 119 69 2 1 113 352 468 1.83e-14 74.3
MS.gene53385.t1 AT4G23320 35.294 119 69 2 1 113 352 468 1.83e-14 74.3
MS.gene53385.t1 AT1G34300 32.877 146 86 6 1 138 652 793 1.03e-13 72.4
MS.gene53385.t1 AT4G04540 41.379 116 62 5 1 115 522 632 2.56e-13 71.2
MS.gene53385.t1 AT4G04540 41.379 116 62 5 1 115 493 603 2.90e-13 70.9
MS.gene53385.t1 AT1G53430 39.252 107 58 2 1 102 838 942 3.37e-13 70.9
MS.gene53385.t1 AT1G53430 39.252 107 58 2 1 102 797 901 3.93e-13 70.9
MS.gene53385.t1 AT4G04490 38.281 128 65 5 1 117 509 633 4.00e-13 70.5
MS.gene53385.t1 AT4G04570 38.400 125 69 5 1 124 517 634 8.11e-13 69.7
MS.gene53385.t1 AT1G53440 38.318 107 59 2 1 102 836 940 9.51e-13 69.7
MS.gene53385.t1 AT4G21230 35.772 123 65 3 1 115 502 618 1.43e-12 68.9
MS.gene53385.t1 AT1G53420 36.364 110 63 2 1 105 793 900 3.38e-12 67.8
MS.gene53385.t1 AT3G14840 37.383 107 60 2 1 102 841 945 3.94e-12 67.8
MS.gene53385.t1 AT4G23290 33.613 119 71 2 1 113 489 605 6.53e-12 66.6
MS.gene53385.t1 AT5G24080 31.452 124 76 3 1 119 699 818 7.24e-12 66.6
MS.gene53385.t1 AT4G23290 33.613 119 71 2 1 113 532 648 7.84e-12 66.6
MS.gene53385.t1 AT5G24080 31.452 124 76 3 1 119 572 691 9.60e-12 66.2
MS.gene53385.t1 AT5G24080 31.452 124 76 3 1 119 572 691 9.60e-12 66.2
MS.gene53385.t1 AT4G11900 34.167 120 69 3 1 115 706 820 2.03e-11 65.5
MS.gene53385.t1 AT4G11900 34.167 120 69 3 1 115 637 751 2.25e-11 65.1
MS.gene53385.t1 AT4G23280 31.933 119 73 2 1 113 463 579 5.62e-11 63.9
MS.gene53385.t1 AT4G23280 31.933 119 73 2 1 113 513 629 7.03e-11 63.5
MS.gene53385.t1 AT4G23260 31.092 119 74 2 1 113 497 613 7.47e-11 63.5
MS.gene53385.t1 AT4G23280 31.933 119 73 2 1 113 511 627 7.48e-11 63.5
MS.gene53385.t1 AT4G32300 38.739 111 54 4 1 102 661 766 7.73e-11 63.5
MS.gene53385.t1 AT3G17420 32.432 111 68 2 1 106 323 431 8.58e-11 63.2
MS.gene53385.t1 AT4G23260 31.092 119 74 2 1 113 508 624 9.02e-11 63.2

Find 54 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGCTGATGTTTATAGTTT+TGG 0.204782 4.1:-13886480 MS.gene53385:CDS
AAGATTATATAGAGAATTAT+TGG 0.213784 4.1:-13886419 MS.gene53385:CDS
TGCCAGGTTAAGAGAGATTC+AGG 0.266589 4.1:+13886183 None:intergenic
CAGATTCATTGACACAAGTT+TGG 0.282213 4.1:-13885685 MS.gene53385:CDS
GAAGGAAAATTGTTCAAGAT+TGG 0.330399 4.1:-13884801 MS.gene53385:CDS
GGCAAGATTACTTTGCTAGA+TGG 0.352379 4.1:-13886398 MS.gene53385:CDS
TTCATTGAGGGGCGATCATC+TGG 0.364908 4.1:+13886267 None:intergenic
TGCTTACACAACCACCTCTA+TGG 0.365363 4.1:-13884580 MS.gene53385:intron
TTCCGATGAGGATGAAGTTT+CGG 0.370322 4.1:-13885036 MS.gene53385:CDS
GCTTCAGATTTGCTGTTATG+AGG 0.386096 4.1:-13884069 None:intergenic
CTCGTGTCGTCGACAAATCA+AGG 0.427985 4.1:-13884652 MS.gene53385:CDS
ATCTGCTAACGTCCTTTGCC+AGG 0.438352 4.1:+13886167 None:intergenic
ATCGGAAATTGCAACTTTGT+CGG 0.438491 4.1:+13884974 None:intergenic
ATCTATCACTTCATTCATTG+AGG 0.443846 4.1:+13886254 None:intergenic
TGGAATGATGTTGATGGAAA+TGG 0.446700 4.1:-13886460 MS.gene53385:CDS
ACGATTACACCTTCAATTGA+AGG 0.455930 4.1:-13884819 MS.gene53385:CDS
GTTAAGAGAGATTCAGGATC+AGG 0.460602 4.1:+13886189 None:intergenic
TTCGTTATCTGAATCTGTTA+CGG 0.465411 4.1:-13884760 MS.gene53385:CDS
ATGGATTTATGATCAATTTG+AGG 0.466465 4.1:-13886379 MS.gene53385:CDS
GATTCAGGATCAGGTGGAAG+TGG 0.472491 4.1:+13886198 None:intergenic
GAAGGTGTAATCGTCGTGAT+TGG 0.477945 4.1:+13884828 None:intergenic
ATGTTCTTGTACAGCAACTC+TGG 0.487497 4.1:+13886519 None:intergenic
CTTCAGATTTGCTGTTATGA+GGG 0.493610 4.1:-13884068 None:intergenic
GAAGTGATAGATATGCTTGA+AGG 0.495281 4.1:-13886243 MS.gene53385:CDS
TCTATCACTTCATTCATTGA+GGG 0.502101 4.1:+13886255 None:intergenic
AACTTTGTCGGAATCTTCAA+CGG 0.542646 4.1:+13884986 None:intergenic
ATCTGAATCTGTTACGGTGA+CGG 0.551321 4.1:-13884754 MS.gene53385:CDS
AACCGAAACTTCATCCTCAT+CGG 0.555405 4.1:+13885034 None:intergenic
CGTCGACAAATCAAGGAAAG+AGG 0.556964 4.1:-13884645 MS.gene53385:CDS
AGTTTCATCTGAATCTTCAT+CGG 0.566452 4.1:+13884956 None:intergenic
GAGGAGCTCAATTCTGCAGA+AGG 0.570253 4.1:+13884933 None:intergenic
GGTGGAAGTGGAAGGTGTTG+TGG 0.571266 4.1:+13886210 None:intergenic
AAGAGAGATTCAGGATCAGG+TGG 0.571443 4.1:+13886192 None:intergenic
AAGAGGGCACCTAGCTGCAA+TGG 0.571514 4.1:-13884628 MS.gene53385:CDS
CAGGATCAGGTGGAAGTGGA+AGG 0.583464 4.1:+13886202 None:intergenic
TTGCAATCGTCTGAGACGAC+GGG 0.584710 4.1:-13884603 MS.gene53385:CDS
AGAAAGAAACTTTACCATAG+AGG 0.589858 4.1:+13884566 None:intergenic
GTTGCAATCGTCTGAGACGA+CGG 0.592350 4.1:-13884604 MS.gene53385:CDS
ATCGTCGTGATTGGAAACAC+TGG 0.595741 4.1:+13884837 None:intergenic
CTATCACTTCATTCATTGAG+GGG 0.597502 4.1:+13886256 None:intergenic
ATCCTGAATCTCTCTTAACC+TGG 0.601661 4.1:-13886185 MS.gene53385:CDS
GTCGACAAATCAAGGAAAGA+GGG 0.609988 4.1:-13884644 MS.gene53385:CDS
AATCTCTCTTAACCTGGCAA+AGG 0.613663 4.1:-13886179 MS.gene53385:CDS
TGGAAACACTGGAATAGCAT+CGG 0.614957 4.1:+13884848 None:intergenic
TTGAACTGTGATCAAATCAG+AGG 0.616683 4.1:+13884914 None:intergenic
AAGAAACTTTACCATAGAGG+TGG 0.620189 4.1:+13884569 None:intergenic
ATTGCAACACCATTGCAGCT+AGG 0.627459 4.1:+13884619 None:intergenic
GAGTTGCTGTACAAGAACAT+TGG 0.631951 4.1:-13886516 MS.gene53385:CDS
TTTCCTGCAAAAGCACGTTG+TGG 0.636258 4.1:-13884105 MS.gene53385:CDS
AGATACAAACAATGGAACAC+AGG 0.640860 4.1:-13886349 MS.gene53385:CDS
GAAACAATAGATACAAACAA+TGG 0.649053 4.1:-13886357 MS.gene53385:CDS
TTCCGTTGAAGATTCCGATG+AGG 0.673326 4.1:-13885048 MS.gene53385:intron
TCTGCGATCGTGTATCAATG+TGG 0.676695 4.1:-13884693 MS.gene53385:CDS
ATGATGTTGATGGAAATGGC+AGG 0.716597 4.1:-13886456 MS.gene53385:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATTATATAGAGAATTAT+TGG - chr4.1:13884177-13884196 MS.gene53385:intron 15.0%
!! AATAGATTGATAAAAGATAA+TGG + chr4.1:13885379-13885398 None:intergenic 15.0%
!! ATAGTGAATAAATCATAGTT+AGG - chr4.1:13885254-13885273 MS.gene53385:intron 20.0%
!!! ACTTTTTTTTCCATGTATTT+TGG - chr4.1:13884543-13884562 MS.gene53385:intron 20.0%
! ATGGATTTATGATCAATTTG+AGG - chr4.1:13884217-13884236 MS.gene53385:intron 25.0%
! CACAACTAAACTCTATATAT+AGG - chr4.1:13885335-13885354 MS.gene53385:intron 25.0%
! CGTAAATAAACTAATCATAC+AGG - chr4.1:13884960-13884979 MS.gene53385:CDS 25.0%
! GAAACAATAGATACAAACAA+TGG - chr4.1:13884239-13884258 MS.gene53385:intron 25.0%
! GTTAGGTTATATGTAAACTA+TGG - chr4.1:13885271-13885290 MS.gene53385:intron 25.0%
! TAATACTGTAATGTTCTTGA+AGG - chr4.1:13884997-13885016 MS.gene53385:CDS 25.0%
! TTTAACCGAAAAATCTAAGA+TGG + chr4.1:13884858-13884877 None:intergenic 25.0%
!! AAAGCTGATGTTTATAGTTT+TGG - chr4.1:13884116-13884135 MS.gene53385:CDS 25.0%
!! AACAATTTTCCTTCAATTGA+AGG + chr4.1:13885789-13885808 None:intergenic 25.0%
!! ATTCTAACTCTAGTTTGATT+TGG - chr4.1:13884832-13884851 MS.gene53385:CDS 25.0%
!! TCTGAATACGAATTCATTTT+TGG - chr4.1:13885708-13885727 MS.gene53385:intron 25.0%
!! TTTTACGAAACCAAAATACA+TGG + chr4.1:13884556-13884575 None:intergenic 25.0%
!!! GAATTCATTTTTGGTAGTTT+CGG - chr4.1:13885717-13885736 MS.gene53385:intron 25.0%
AGAAAGAAACTTTACCATAG+AGG + chr4.1:13886033-13886052 None:intergenic 30.0%
AGTTATCATGCAAACGTTAA+AGG - chr4.1:13884501-13884520 MS.gene53385:intron 30.0%
AGTTTCATCTGAATCTTCAT+CGG + chr4.1:13885643-13885662 None:intergenic 30.0%
ATCACTATGACTATTATCCT+TGG - chr4.1:13885463-13885482 MS.gene53385:intron 30.0%
ATCTATCACTTCATTCATTG+AGG + chr4.1:13884345-13884364 None:intergenic 30.0%
ATGAATGAAATTGGCACTAT+TGG - chr4.1:13884471-13884490 MS.gene53385:intron 30.0%
ATTGCATGTTCCATATGTTT+CGG - chr4.1:13884731-13884750 MS.gene53385:CDS 30.0%
CATATGGAACATGCAATATT+AGG + chr4.1:13884728-13884747 None:intergenic 30.0%
GAAGGAAAATTGTTCAAGAT+TGG - chr4.1:13885795-13885814 MS.gene53385:intron 30.0%
GTTATCATGCAAACGTTAAA+GGG - chr4.1:13884502-13884521 MS.gene53385:intron 30.0%
TCACTATGACTATTATCCTT+GGG - chr4.1:13885464-13885483 MS.gene53385:intron 30.0%
TCGATGAGAATGAATGAAAT+TGG - chr4.1:13884462-13884481 MS.gene53385:intron 30.0%
TCTATCACTTCATTCATTGA+GGG + chr4.1:13884344-13884363 None:intergenic 30.0%
TTCGTTATCTGAATCTGTTA+CGG - chr4.1:13885836-13885855 MS.gene53385:intron 30.0%
TTTAACGTTTGCATGATAAC+TGG + chr4.1:13884502-13884521 None:intergenic 30.0%
! ATTTTTCGTTTCTTTCTGCT+TGG - chr4.1:13886243-13886262 MS.gene53385:CDS 30.0%
! TTTGGCCATCTTAGATTTTT+CGG - chr4.1:13884850-13884869 MS.gene53385:CDS 30.0%
!! TCACTTTTACACAATCGTTT+TGG - chr4.1:13886437-13886456 MS.gene53385:CDS 30.0%
!!! TAGTTTTGGAATGATGTTGA+TGG - chr4.1:13884130-13884149 MS.gene53385:intron 30.0%
AACTTTGTCGGAATCTTCAA+CGG + chr4.1:13885613-13885632 None:intergenic 35.0%
AAGAAACTTTACCATAGAGG+TGG + chr4.1:13886030-13886049 None:intergenic 35.0%
AAGTAACCATAGAGACTCAT+AGG + chr4.1:13885408-13885427 None:intergenic 35.0%
ACGATTACACCTTCAATTGA+AGG - chr4.1:13885777-13885796 MS.gene53385:intron 35.0%
AGATACAAACAATGGAACAC+AGG - chr4.1:13884247-13884266 MS.gene53385:intron 35.0%
ATCGGAAATTGCAACTTTGT+CGG + chr4.1:13885625-13885644 None:intergenic 35.0%
CACAGTCTGAAGATATCAAA+TGG - chr4.1:13884761-13884780 MS.gene53385:CDS 35.0%
CAGATTCATTGACACAAGTT+TGG - chr4.1:13884911-13884930 MS.gene53385:CDS 35.0%
CATTTGATATCTTCAGACTG+TGG + chr4.1:13884763-13884782 None:intergenic 35.0%
CTATCACTTCATTCATTGAG+GGG + chr4.1:13884343-13884362 None:intergenic 35.0%
GAAGTGATAGATATGCTTGA+AGG - chr4.1:13884353-13884372 MS.gene53385:intron 35.0%
TCATTGACAACTTAAGACCA+GGG - chr4.1:13885102-13885121 MS.gene53385:intron 35.0%
TTGAACTGTGATCAAATCAG+AGG + chr4.1:13885685-13885704 None:intergenic 35.0%
! AAAGCAGAGCATTTAGCTAA+AGG + chr4.1:13884688-13884707 None:intergenic 35.0%
! AATATTAGGAGTATAGCCAG+AGG + chr4.1:13884714-13884733 None:intergenic 35.0%
! CTATGGTTAGTTAGTTAGCT+TGG - chr4.1:13885288-13885307 MS.gene53385:intron 35.0%
! TGGAATGATGTTGATGGAAA+TGG - chr4.1:13884136-13884155 MS.gene53385:intron 35.0%
!!! GTGGTTTTAACCGAAACATA+TGG + chr4.1:13884744-13884763 None:intergenic 35.0%
AACCGAAACTTCATCCTCAT+CGG + chr4.1:13885565-13885584 None:intergenic 40.0%
AAGTATGTTCAATCAGTCCC+TGG + chr4.1:13885122-13885141 None:intergenic 40.0%
AATCAAAGAAAACGCTCCAC+CGG + chr4.1:13886102-13886121 None:intergenic 40.0%
AATCTCTCTTAACCTGGCAA+AGG - chr4.1:13884417-13884436 MS.gene53385:intron 40.0%
ACCACAGTCTGAATTCAGAA+TGG - chr4.1:13884878-13884897 MS.gene53385:CDS 40.0%
ACCATTCTGAATTCAGACTG+TGG + chr4.1:13884882-13884901 None:intergenic 40.0%
ACGCTATATATCTCCAAGAG+AGG - chr4.1:13884784-13884803 MS.gene53385:CDS 40.0%
ATCCTCATCGGAATCTTCAA+CGG + chr4.1:13885553-13885572 None:intergenic 40.0%
ATCCTGAATCTCTCTTAACC+TGG - chr4.1:13884411-13884430 MS.gene53385:intron 40.0%
ATCTGAATCTGTTACGGTGA+CGG - chr4.1:13885842-13885861 MS.gene53385:intron 40.0%
ATCTTTACAACGTTCGCCTA+AGG - chr4.1:13884656-13884675 MS.gene53385:CDS 40.0%
ATGTTCTTGTACAGCAACTC+TGG + chr4.1:13884080-13884099 None:intergenic 40.0%
CGCTATATATCTCCAAGAGA+GGG - chr4.1:13884785-13884804 MS.gene53385:CDS 40.0%
GAGTTGCTGTACAAGAACAT+TGG - chr4.1:13884080-13884099 MS.gene53385:CDS 40.0%
GATTCTCCTATGAGTCTCTA+TGG - chr4.1:13885399-13885418 MS.gene53385:intron 40.0%
GCTATATATCTCCAAGAGAG+GGG - chr4.1:13884786-13884805 MS.gene53385:CDS 40.0%
GGCAAGATTACTTTGCTAGA+TGG - chr4.1:13884198-13884217 MS.gene53385:intron 40.0%
GTCATTGACAACTTAAGACC+AGG - chr4.1:13885101-13885120 MS.gene53385:intron 40.0%
GTCGACAAATCAAGGAAAGA+GGG - chr4.1:13885952-13885971 MS.gene53385:intron 40.0%
GTTAAGAGAGATTCAGGATC+AGG + chr4.1:13884410-13884429 None:intergenic 40.0%
TCGTTTCTTTCTGCTTGGTT+TGG - chr4.1:13886248-13886267 MS.gene53385:CDS 40.0%
TGGAAACACTGGAATAGCAT+CGG + chr4.1:13885751-13885770 None:intergenic 40.0%
TTACGAATCAGAGAATCCCA+AGG + chr4.1:13885483-13885502 None:intergenic 40.0%
TTCCGATGAGGATGAAGTTT+CGG - chr4.1:13885560-13885579 MS.gene53385:intron 40.0%
! ATGATGTTGATGGAAATGGC+AGG - chr4.1:13884140-13884159 MS.gene53385:intron 40.0%
! GGATTCTCTGATTCGTAACA+TGG - chr4.1:13885485-13885504 MS.gene53385:intron 40.0%
!! TGAAGAAGCTTTTGAGAGCA+AGG - chr4.1:13886290-13886309 MS.gene53385:CDS 40.0%
!! TGGAGCGTTTTCTTTGATTC+TGG - chr4.1:13886103-13886122 MS.gene53385:intron 40.0%
AAGAGAGATTCAGGATCAGG+TGG + chr4.1:13884407-13884426 None:intergenic 45.0%
AATAGTCATAGTGATCCGCG+AGG + chr4.1:13885458-13885477 None:intergenic 45.0%
AATGCTCTGCTTTGATCCTC+TGG - chr4.1:13884695-13884714 MS.gene53385:CDS 45.0%
AGCTTCTGTAACAGCGTGTA+TGG + chr4.1:13886222-13886241 None:intergenic 45.0%
AGTGCATGGCGATATTCATG+AGG + chr4.1:13885432-13885451 None:intergenic 45.0%
ATCAAAGAAAACGCTCCACC+GGG + chr4.1:13886101-13886120 None:intergenic 45.0%
ATCGTCGTGATTGGAAACAC+TGG + chr4.1:13885762-13885781 None:intergenic 45.0%
ATTGCAACACCATTGCAGCT+AGG + chr4.1:13885980-13885999 None:intergenic 45.0%
CGTCATCATCTTCATCCCTA+GGG + chr4.1:13885524-13885543 None:intergenic 45.0%
CGTCGACAAATCAAGGAAAG+AGG - chr4.1:13885951-13885970 MS.gene53385:intron 45.0%
TAGCTAAAGGCAGAAGCCTT+AGG + chr4.1:13884675-13884694 None:intergenic 45.0%
TCGGATTTCGTATTCGATCC+CGG - chr4.1:13886080-13886099 MS.gene53385:intron 45.0%
TCGTCATCATCTTCATCCCT+AGG + chr4.1:13885525-13885544 None:intergenic 45.0%
TCTGCGATCGTGTATCAATG+TGG - chr4.1:13885903-13885922 MS.gene53385:intron 45.0%
TGCCAGGTTAAGAGAGATTC+AGG + chr4.1:13884416-13884435 None:intergenic 45.0%
TGCTTACACAACCACCTCTA+TGG - chr4.1:13886016-13886035 MS.gene53385:intron 45.0%
TGTCACACGCTTCAGTTGTT+CGG - chr4.1:13886061-13886080 MS.gene53385:intron 45.0%
TTCCGTTGAAGATTCCGATG+AGG - chr4.1:13885548-13885567 MS.gene53385:intron 45.0%
! GAAGGTGTAATCGTCGTGAT+TGG + chr4.1:13885771-13885790 None:intergenic 45.0%
! TTTCCTGCAAAAGCACGTTG+TGG - chr4.1:13886491-13886510 MS.gene53385:CDS 45.0%
ATCACATCCCTTCCCGTTTG+TGG - chr4.1:13886150-13886169 MS.gene53385:intron 50.0%
ATCTGCTAACGTCCTTTGCC+AGG + chr4.1:13884432-13884451 None:intergenic 50.0%
CTCGTGTCGTCGACAAATCA+AGG - chr4.1:13885944-13885963 MS.gene53385:intron 50.0%
GAGGAGCTCAATTCTGCAGA+AGG + chr4.1:13885666-13885685 None:intergenic 50.0%
GATTCAGGATCAGGTGGAAG+TGG + chr4.1:13884401-13884420 None:intergenic 50.0%
GATTTCGTATTCGATCCCGG+TGG - chr4.1:13886083-13886102 MS.gene53385:intron 50.0%
GGTATCAGAGCGATACCCTA+GGG - chr4.1:13885506-13885525 MS.gene53385:intron 50.0%
TGGTATCAGAGCGATACCCT+AGG - chr4.1:13885505-13885524 MS.gene53385:intron 50.0%
TTCATTGAGGGGCGATCATC+TGG + chr4.1:13884332-13884351 None:intergenic 50.0%
! GTTGCAATCGTCTGAGACGA+CGG - chr4.1:13885992-13886011 MS.gene53385:intron 50.0%
! TCTCCACAACGTGCTTTTGC+AGG + chr4.1:13886497-13886516 None:intergenic 50.0%
! TTGCAATCGTCTGAGACGAC+GGG - chr4.1:13885993-13886012 MS.gene53385:intron 50.0%
ACATCCCTTCCCGTTTGTGG+TGG - chr4.1:13886153-13886172 MS.gene53385:intron 55.0%
CAGGATCAGGTGGAAGTGGA+AGG + chr4.1:13884397-13884416 None:intergenic 55.0%
CCAACATGTGACCCCTCTCT+TGG + chr4.1:13884800-13884819 None:intergenic 55.0%
CCAAGAGAGGGGTCACATGT+TGG - chr4.1:13884797-13884816 MS.gene53385:CDS 55.0%
CCAAGGGATCCACCACAAAC+GGG + chr4.1:13886165-13886184 None:intergenic 55.0%
CCCAAGGGATCCACCACAAA+CGG + chr4.1:13886166-13886185 None:intergenic 55.0%
CTTTCTTGCCGCGATCCCAA+GGG + chr4.1:13886181-13886200 None:intergenic 55.0%
GGATCCACCACAAACGGGAA+GGG + chr4.1:13886160-13886179 None:intergenic 55.0%
!! AAGAGGGCACCTAGCTGCAA+TGG - chr4.1:13885968-13885987 MS.gene53385:intron 55.0%
!! CCGTTTGTGGTGGATCCCTT+GGG - chr4.1:13886163-13886182 MS.gene53385:intron 55.0%
!! GGTGGAAGTGGAAGGTGTTG+TGG + chr4.1:13884389-13884408 None:intergenic 55.0%
CCTTGGGATCGCGGCAAGAA+AGG - chr4.1:13886179-13886198 MS.gene53385:CDS 60.0%
CCTTTCTTGCCGCGATCCCA+AGG + chr4.1:13886182-13886201 None:intergenic 60.0%
GGGATCCACCACAAACGGGA+AGG + chr4.1:13886161-13886180 None:intergenic 60.0%
TCGCCATGCACTCTTCCTCG+CGG - chr4.1:13885440-13885459 MS.gene53385:intron 60.0%
! GATCCGCGAGGAAGAGTGCA+TGG + chr4.1:13885446-13885465 None:intergenic 60.0%
! TGGTGGATCCCTTGGGATCG+CGG - chr4.1:13886170-13886189 MS.gene53385:intron 60.0%
!! CCCGTTTGTGGTGGATCCCT+TGG - chr4.1:13886162-13886181 MS.gene53385:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 13884071 13886547 13884071 ID=MS.gene53385
chr4.1 mRNA 13884071 13886547 13884071 ID=MS.gene53385.t1;Parent=MS.gene53385
chr4.1 exon 13886177 13886547 13886177 ID=MS.gene53385.t1.exon1;Parent=MS.gene53385.t1
chr4.1 CDS 13886177 13886547 13886177 ID=cds.MS.gene53385.t1;Parent=MS.gene53385.t1
chr4.1 exon 13885658 13885704 13885658 ID=MS.gene53385.t1.exon2;Parent=MS.gene53385.t1
chr4.1 CDS 13885658 13885704 13885658 ID=cds.MS.gene53385.t1;Parent=MS.gene53385.t1
chr4.1 exon 13884581 13885059 13884581 ID=MS.gene53385.t1.exon3;Parent=MS.gene53385.t1
chr4.1 CDS 13884581 13885059 13884581 ID=cds.MS.gene53385.t1;Parent=MS.gene53385.t1
chr4.1 exon 13884071 13884148 13884071 ID=MS.gene53385.t1.exon4;Parent=MS.gene53385.t1
chr4.1 CDS 13884071 13884148 13884071 ID=cds.MS.gene53385.t1;Parent=MS.gene53385.t1
Gene Sequence

>MS.gene53385

ATGGCTCCAGAGTTGCTGTACAAGAACATTGGAAGCATATCTCACAAAGCTGATGTTTATAGTTTTGGAATGATGTTGATGGAAATGGCAGGTAGAAAAAAGAACAAAGATTATATAGAGAATTATTGGCAAGATTACTTTGCTAGATGGATTTATGATCAATTTGAGGAAACAATAGATACAAACAATGGAACACAGGAGAAGAAAAATATTGCTATGAAGATGATTGTGATAGCATTAAAATGCATACAAATGAAACCAGATGATCGCCCCTCAATGAATGAAGTGATAGATATGCTTGAAGGAGATGAAGTTCCACAACACCTTCCACTTCCACCTGATCCTGAATCTCTCTTAACCTGGCAAAGGACGTTAGCAGATGCAAATGAATTCGATGAGAATGAATGAAATTGGCACTATTGGTTGTTCCAGTTATCATGCAAACGTTAAAGGGTACTAGTAGATAAGTATTACTTTTTTTTCCATGTATTTTGGTTTCGTAAAATGTAATCTATCTATTATAATGATAATTTGCATCATGAACAATCTTATAGACTTTCATTATGCAGTTGAAGCAAGATCTACATCTTTACAACGTTCGCCTAAGGCTTCTGCCTTTAGCTAAATGCTCTGCTTTGATCCTCTGGCTATACTCCTAATATTGCATGTTCCATATGTTTCGGTTAAAACCACAGTCTGAAGATATCAAATGGACGCTATATATCTCCAAGAGAGGGGTCACATGTTGGACATGTCTTTTAATTCTAACTCTAGTTTGATTTGGCCATCTTAGATTTTTCGGTTAAAACCACAGTCTGAATTCAGAATGGTTTGAAAAAACAGATTCATTGACACAAGTTTGGACAGTTTAATGATAAAAACAAACTCACGTAAATAAACTAATCATACAGGTGTATTTCTAACATTAATACTGTAATGTTCTTGAAGGACAAAAAAATGTACATTTAATTTCATTCACTATCAAAGCTTTTTTAGAGCAAACATTTTATTTATATATACTTATCACTGAGTCATTGACAACTTAAGACCAGGGACTGATTGAACATACTTATCATACTCGAATTATATATACATTTTTTAGTATTGTTCACAGTCAATTTATTTTGATTTCTACATCATATCATGTCAAAGCATGTTTATAAATAAATCAATCTATTAGAGTATAGTGAATAAATCATAGTTAGGTTATATGTAAACTATGGTTAGTTAGTTAGCTTGGTTAATAGTTAGTTAGAGTTAGTTACACAACTAAACTCTATATATAGGAACACTCTTGTATCATTTCCATTATCTTTTATCAATCTATTGATTCTCCTATGAGTCTCTATGGTTACTTACCTCATGAATATCGCCATGCACTCTTCCTCGCGGATCACTATGACTATTATCCTTGGGATTCTCTGATTCGTAACATGGTATCAGAGCGATACCCTAGGGATGAAGATGATGACGACGCTTCCGTTGAAGATTCCGATGAGGATGAAGTTTCGGTTGAAGATTCTAACGAAGTTGCAATTTCCGTTGAAGATTCCGACAAAGTTGCAATTTCCGATGAAGATTCAGATGAAACTCCTTCTGCAGAATTGAGCTCCTCTGATTTGATCACAGTTCAAGATTCTGAATACGAATTCATTTTTGGTAGTTTCGGTCAAGCTTCCGATGCTATTCCAGTGTTTCCAATCACGACGATTACACCTTCAATTGAAGGAAAATTGTTCAAGATTGGAGAAATTGAGTGTTCTCTTTCGTTATCTGAATCTGTTACGGTGACGGAACAACAAAGCTCGAATCTAGAAATTCAAGATTTCTCTTCTGCGATCGTGTATCAATGTGGAAACGACGTCGATCAATTCTCGTGTCGTCGACAAATCAAGGAAAGAGGGCACCTAGCTGCAATGGTGTTGCAATCGTCTGAGACGACGGGTGCTTACACAACCACCTCTATGGTAAAGTTTCTTTCTGACTTTTTTGTCACACGCTTCAGTTGTTCGGATTTCGTATTCGATCCCGGTGGAGCGTTTTCTTTGATTCTGGTGTGTTTTGATCTCAAAGTTCTTCATCACATCCCTTCCCGTTTGTGGTGGATCCCTTGGGATCGCGGCAAGAAAGGTCTGTATTCATTCGATTCCATACACGCTGTTACAGAAGCTCATTTTTCGTTTCTTTCTGCTTGGTTTGGTTCAAAACTACGACGTCGTTGAAGAAGCTTTTGAGAGCAAGGATGTATTGTTTCGCAATCTCAATTCAGAACTCAGACAGATCAAGAAGCATGATTGCATCATTTTGTTCTTCAATTTCAACATACAACAGAAAAAAAATCACGTTTGCAGATTTACTTCGCAAAATCACTTTTACACAATCGTTTTGGCATGTTGCAGCATCAAGCTTTGTTTATGTTCTTTCCTGCAAAAGCACGTTGTGGAGAAGTTATTTTTGCTTCAGATTTGCTGTTATGA
Protein sequence

>MS.gene53385.t1

MAPELLYKNIGSISHKADVYSFGMMLMEMAGRKKNKDYIENYWQDYFARWIYDQFEETIDTNNGTQEKKNIAMKMIVIALKCIQMKPDDRPSMNEVIDMLEGDEVPQHLPLPPDPESLLTWQRTFIDTSLDSLMIKTNSHSDEDEVSVEDSNEVAISVEDSDKVAISDEDSDETPSAELSSSDLITVQDSEYEFIFGSFGQASDAIPVFPITTITPSIEGKLFKIGEIECSLSLSESVTVTEQQSSNLEIQDFSSAIVYQCGNDVDQFSCRRQIKERGHLAAMVLQSSETTGAYTTTSMHQALFMFFPAKARCGEVIFASDLLL