AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53441

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53441.t1 MTR_8g086410 96.350 274 5 2 1 272 109 379 0.0 545
MS.gene53441.t1 MTR_8g086410 96.350 274 5 2 1 272 111 381 0.0 545
MS.gene53441.t1 MTR_8g086410 93.950 281 5 3 1 272 111 388 0.0 538
MS.gene53441.t1 MTR_2g016220 45.051 293 85 9 1 272 89 326 1.06e-64 207
MS.gene53441.t1 MTR_2g016220 45.051 293 85 9 1 272 89 326 1.77e-64 206
MS.gene53441.t1 MTR_2g016220 44.444 297 85 10 1 272 89 330 1.47e-62 201
MS.gene53441.t1 MTR_2g084230 46.392 194 76 7 1 194 97 262 3.15e-38 136
MS.gene53441.t1 MTR_2g084230 46.392 194 76 7 1 194 97 262 8.61e-38 137
MS.gene53441.t1 MTR_7g069660 79.710 69 13 1 2 70 72 139 7.95e-32 120
MS.gene53441.t1 MTR_2g023580 55.056 89 36 2 1 89 55 139 1.55e-26 103
MS.gene53441.t1 MTR_0223s0040 66.667 63 21 0 1 63 18 80 2.30e-24 99.4
MS.gene53441.t1 MTR_1g112370 68.421 57 18 0 1 57 22 78 1.76e-22 95.1
MS.gene53441.t1 MTR_6g071625 69.643 56 17 0 2 57 203 258 3.91e-20 89.0
MS.gene53441.t1 MTR_1g021520 66.129 62 20 1 2 62 127 188 7.09e-20 87.4
MS.gene53441.t1 MTR_3g450310 57.353 68 24 2 2 68 20 83 7.45e-20 85.5
MS.gene53441.t1 MTR_7g115530 53.247 77 32 1 2 78 219 291 1.68e-19 86.3
MS.gene53441.t1 MTR_5g029470 66.129 62 20 1 2 62 220 281 2.17e-19 87.4
MS.gene53441.t1 MTR_7g098250 66.071 56 19 0 2 57 263 318 2.27e-19 87.8
MS.gene53441.t1 MTR_8g066310 64.407 59 21 0 2 60 174 232 2.36e-19 86.7
MS.gene53441.t1 MTR_7g115530 53.247 77 32 1 2 78 219 291 3.26e-19 85.9
MS.gene53441.t1 MTR_3g064840 69.643 56 17 0 2 57 275 330 4.29e-19 86.7
MS.gene53441.t1 MTR_7g115530 53.247 77 32 1 2 78 219 291 4.62e-19 86.7
MS.gene53441.t1 MTR_1g080330 64.286 56 20 0 2 57 176 231 6.77e-19 84.3
MS.gene53441.t1 MTR_7g115530 64.286 56 20 0 2 57 219 274 7.76e-19 85.1
MS.gene53441.t1 MTR_1g080330 64.286 56 20 0 2 57 176 231 1.01e-18 85.1
MS.gene53441.t1 MTR_7g093030 58.929 56 23 0 3 58 214 269 1.27e-17 81.3
MS.gene53441.t1 MTR_7g115530 62.500 56 21 0 2 57 219 274 2.09e-17 80.1
MS.gene53441.t1 MTR_7g093030 58.929 56 23 0 3 58 214 269 2.64e-17 81.3
MS.gene53441.t1 MTR_7g115530 65.385 52 18 0 2 53 219 270 2.95e-17 80.1
MS.gene53441.t1 MTR_6g032990 62.264 53 20 0 2 54 43 95 5.51e-17 80.5
MS.gene53441.t1 MTR_1g053835 61.818 55 21 0 3 57 260 314 1.36e-16 79.3
MS.gene53441.t1 MTR_5g027440 62.264 53 20 0 2 54 26 78 1.72e-16 77.8
MS.gene53441.t1 MTR_2g086450 58.929 56 23 0 2 57 46 101 1.95e-16 78.6
MS.gene53441.t1 MTR_7g088070 63.636 55 20 0 3 57 52 106 4.42e-16 76.6
MS.gene53441.t1 MTR_1g053830 56.364 55 24 0 3 57 20 74 4.45e-16 72.4
MS.gene53441.t1 MTR_7g088070 63.636 55 20 0 3 57 52 106 4.68e-16 76.6
MS.gene53441.t1 MTR_1g090670 56.452 62 25 1 2 63 45 104 8.49e-16 75.9
MS.gene53441.t1 MTR_2g027800 58.929 56 23 0 2 57 40 95 1.02e-15 75.9
MS.gene53441.t1 MTR_2g027800 60.377 53 21 0 2 54 40 92 1.76e-15 75.1
MS.gene53441.t1 MTR_2g027860 63.636 55 20 0 3 57 51 105 2.16e-15 75.1
MS.gene53441.t1 MTR_5g041350 39.175 97 48 3 2 87 16 112 2.19e-15 75.5
MS.gene53441.t1 MTR_6g444980 53.571 56 26 0 2 57 36 91 2.29e-14 72.4
MS.gene53441.t1 MTR_6g444980 53.571 56 26 0 2 57 36 91 2.47e-14 72.4
MS.gene53441.t1 MTR_1g053800 58.491 53 22 0 3 55 179 231 7.11e-14 70.9
MS.gene53441.t1 MTR_7g068600 59.322 59 21 1 3 58 15 73 1.17e-13 69.3
MS.gene53441.t1 MTR_4g081710 51.786 56 27 0 2 57 18 73 1.58e-13 70.1
MS.gene53441.t1 MTR_7g093010 60.377 53 21 0 3 55 215 267 6.22e-13 68.2
MS.gene53441.t1 MTR_4g021790 51.923 52 24 1 3 54 170 220 3.79e-11 62.4
MS.gene53441.t1 MTR_4g113140 53.846 52 24 0 3 54 237 288 5.07e-11 62.8
MS.gene53441.t1 MTR_4g086835 51.923 52 25 0 3 54 276 327 7.67e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53441.t1 AT2G38300 77.273 66 15 0 1 66 54 119 8.92e-31 117
MS.gene53441.t1 AT2G42660 81.356 59 11 0 1 59 50 108 9.88e-28 107
MS.gene53441.t1 AT2G40260 84.746 59 9 0 2 60 83 141 1.93e-27 109
MS.gene53441.t1 AT4G04605 60.759 79 31 0 1 79 14 92 3.00e-27 105
MS.gene53441.t1 AT2G02060 76.364 55 13 0 1 55 29 83 3.75e-24 98.2
MS.gene53441.t1 AT2G02060 76.364 55 13 0 1 55 29 83 3.75e-24 98.2
MS.gene53441.t1 AT1G14600 65.079 63 22 0 1 63 23 85 1.23e-23 96.7
MS.gene53441.t1 AT5G42630 65.574 61 21 0 2 62 106 166 6.91e-21 88.6
MS.gene53441.t1 AT5G42630 65.574 61 21 0 2 62 106 166 1.46e-20 89.0
MS.gene53441.t1 AT4G04580 60.000 60 24 0 2 61 15 74 1.50e-19 83.6
MS.gene53441.t1 AT1G32240 66.129 62 20 1 2 62 214 275 1.66e-19 87.4
MS.gene53441.t1 AT5G16560 54.118 85 38 1 2 86 220 303 1.76e-19 87.4
MS.gene53441.t1 AT1G32240 66.129 62 20 1 2 62 214 275 2.52e-19 87.0
MS.gene53441.t1 AT4G17695 66.102 59 20 0 2 60 165 223 6.59e-19 85.1
MS.gene53441.t1 AT4G04555 57.377 61 26 0 1 61 14 74 5.51e-18 77.8
MS.gene53441.t1 AT4G28610 63.636 55 20 0 3 57 227 281 1.03e-17 82.4
MS.gene53441.t1 AT5G29000 63.636 55 20 0 3 57 233 287 2.03e-17 80.9
MS.gene53441.t1 AT3G04450 60.000 55 22 0 3 57 240 294 2.03e-17 80.9
MS.gene53441.t1 AT5G45580 62.264 53 20 0 2 54 8 60 2.55e-17 79.7
MS.gene53441.t1 AT5G29000 63.636 55 20 0 3 57 190 244 2.56e-17 81.3
MS.gene53441.t1 AT5G29000 63.636 55 20 0 3 57 190 244 2.56e-17 81.3
MS.gene53441.t1 AT5G29000 63.636 55 20 0 3 57 233 287 3.24e-17 81.3
MS.gene53441.t1 AT5G45580 62.264 53 20 0 2 54 24 76 3.27e-17 79.7
MS.gene53441.t1 AT3G04450 60.000 55 22 0 3 57 240 294 3.50e-17 81.3
MS.gene53441.t1 AT3G04450 60.000 55 22 0 3 57 200 254 3.84e-17 80.9
MS.gene53441.t1 AT3G04450 60.000 55 22 0 3 57 200 254 3.84e-17 80.9
MS.gene53441.t1 AT3G13040 65.455 55 19 0 3 57 243 297 1.38e-16 79.3
MS.gene53441.t1 AT3G13040 65.455 55 19 0 3 57 243 297 1.38e-16 79.3
MS.gene53441.t1 AT3G13040 65.455 55 19 0 3 57 243 297 1.38e-16 79.3
MS.gene53441.t1 AT3G04030 55.357 56 25 0 2 57 46 101 3.54e-16 78.2
MS.gene53441.t1 AT3G04030 55.357 56 25 0 2 57 46 101 4.24e-16 77.8
MS.gene53441.t1 AT5G18240 55.357 56 25 0 2 57 46 101 6.09e-16 77.4
MS.gene53441.t1 AT5G18240 55.357 56 25 0 2 57 46 101 6.09e-16 77.4
MS.gene53441.t1 AT5G18240 55.357 56 25 0 2 57 46 101 6.50e-16 77.4
MS.gene53441.t1 AT5G18240 55.357 56 25 0 2 57 46 101 6.74e-16 77.4
MS.gene53441.t1 AT5G18240 55.357 56 25 0 2 57 46 101 6.74e-16 77.4
MS.gene53441.t1 AT2G01060 63.636 55 20 0 3 57 17 71 1.14e-15 75.5
MS.gene53441.t1 AT5G06800 58.182 55 23 0 3 57 194 248 1.59e-15 75.1
MS.gene53441.t1 AT2G20400 54.286 70 32 0 3 72 233 302 2.01e-15 75.9
MS.gene53441.t1 AT2G20400 55.405 74 26 2 3 72 233 303 2.42e-15 75.5
MS.gene53441.t1 AT3G12730 55.357 56 25 0 2 57 24 79 2.46e-15 73.9
MS.gene53441.t1 AT5G06800 58.182 55 23 0 3 57 194 248 5.67e-15 74.3
MS.gene53441.t1 AT5G06800 58.182 55 23 0 3 57 194 248 5.97e-15 74.3
MS.gene53441.t1 AT1G69580 53.226 62 26 1 2 60 35 96 8.28e-15 73.6
MS.gene53441.t1 AT1G69580 53.226 62 26 1 2 60 35 96 9.27e-15 73.6
MS.gene53441.t1 AT2G06020 53.333 60 28 0 3 62 88 147 9.87e-15 73.2
MS.gene53441.t1 AT4G13640 54.717 53 24 0 2 54 38 90 1.02e-14 72.8
MS.gene53441.t1 AT4G13640 54.717 53 24 0 2 54 38 90 1.03e-14 72.8
MS.gene53441.t1 AT3G24120 54.717 53 24 0 2 54 42 94 1.31e-14 72.8
MS.gene53441.t1 AT3G24120 54.717 53 24 0 2 54 42 94 1.39e-14 72.8
MS.gene53441.t1 AT1G79430 53.571 56 26 0 2 57 35 90 1.68e-14 72.8
MS.gene53441.t1 AT3G04030 53.571 56 25 1 2 57 46 100 1.33e-13 70.5
MS.gene53441.t1 AT3G62670 52.632 57 23 1 2 54 212 268 6.89e-12 65.5
MS.gene53441.t1 AT2G03500 53.846 52 24 0 3 54 235 286 3.94e-11 63.2
MS.gene53441.t1 AT1G25550 53.846 52 24 0 3 54 211 262 6.57e-11 62.4

Find 67 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCAGTTGAAGCACCAATT+TGG 0.203737 4.3:+14309726 None:intergenic
GATGATTTAAGTACCATTCT+TGG 0.272460 4.3:+14307934 None:intergenic
TGATCTTCCTTGTGTATTAA+TGG 0.279777 4.3:+14307860 None:intergenic
ATGATTGTTCATGATATCTA+AGG 0.331312 4.3:+14307734 None:intergenic
ATCAGTTGAAGCACCAATTT+GGG 0.338016 4.3:+14309727 None:intergenic
TATAGTTTACCTTGAATTGA+TGG 0.341831 4.3:+14308034 None:intergenic
GAACCAGCAGCTTCAAGTTA+AGG 0.347972 4.3:-14307826 MS.gene53441:CDS
TGCAGGAGCAACACCCAAAT+TGG 0.368712 4.3:-14309740 MS.gene53441:intron
CACAGATCAACCCATGAATT+AGG 0.371135 4.3:-14308221 MS.gene53441:CDS
TCATGAACAATCATCCATAT+TGG 0.374740 4.3:-14307725 MS.gene53441:CDS
AAGCTGCTGGTTCAAAGTTA+TGG 0.381198 4.3:+14307836 None:intergenic
AGAAATTGGCTTGGTCTCAT+AGG 0.398094 4.3:+14307777 None:intergenic
GGTACTTAAATCATCAATCT+TGG 0.408196 4.3:-14307926 MS.gene53441:CDS
TCATGTCAAGAGTCACTTGC+AGG 0.410009 4.3:-14309680 MS.gene53441:intron
GTGATAAGGATGAGGGATGC+AGG 0.412034 4.3:+14308078 None:intergenic
ATGTTACCTTAATCCGAAAC+GGG 0.413271 4.3:+14307541 None:intergenic
TATATATATACTTACTCTCT+TGG 0.424725 4.3:+14309878 None:intergenic
TTCTCTTCAAGAAATTGGCT+TGG 0.425569 4.3:+14307768 None:intergenic
AGACCCGCTGAGGTGAGCTT+TGG 0.428693 4.3:-14307648 MS.gene53441:CDS
GAGAAATGGAACCTAATTCA+TGG 0.443905 4.3:+14308210 None:intergenic
TGTGTATTAATGGCTGTTGA+TGG 0.444504 4.3:+14307870 None:intergenic
AGCAAGAAACTTGACGAGGC+TGG 0.448557 4.3:-14308989 MS.gene53441:CDS
TTTCCAAAGCTCACCTCAGC+GGG 0.450601 4.3:+14307645 None:intergenic
ATAAAAGAGTGATGAAGATC+AGG 0.470459 4.3:+14309925 None:intergenic
ATGGGCTACATGGTGAGAAA+TGG 0.471248 4.3:+14308196 None:intergenic
AATGTTACCTTAATCCGAAA+CGG 0.472630 4.3:+14307540 None:intergenic
CAACATTTCAAGATGGCAAA+TGG 0.480821 4.3:-14308155 MS.gene53441:CDS
CCTCGTCAAGTTTCTTGCTC+CGG 0.482077 4.3:+14308993 None:intergenic
TATCCTTAACTTGAAGCTGC+TGG 0.484791 4.3:+14307823 None:intergenic
ATTCAAAGCCCATCAATTCA+AGG 0.485522 4.3:-14308043 MS.gene53441:intron
ATAGTTTACCTTGAATTGAT+GGG 0.489818 4.3:+14308035 None:intergenic
GATCAAAGATGCCTAGGCTT+AGG 0.490445 4.3:-14309954 None:intergenic
ATGCATGCTGTTGAAAGGCT+TGG 0.493618 4.3:-14309904 MS.gene53441:CDS
AATTCATTGCTCATGTAGCT+TGG 0.505208 4.3:+14307900 None:intergenic
AATTTCTTGAAGAGAAAAGA+TGG 0.506639 4.3:-14307761 MS.gene53441:CDS
TCATGGGTTGATCTGTGTGT+TGG 0.507129 4.3:+14308227 None:intergenic
CAAAGATGCCTAGGCTTAGG+TGG 0.512420 4.3:-14309951 None:intergenic
TTGAACTATTAAAATAGTTG+TGG 0.532058 4.3:+14307580 None:intergenic
TTGGAAACAACACAAGAAGC+AGG 0.535242 4.3:-14307629 MS.gene53441:CDS
ATGTTAGATCAAAGATGCCT+AGG 0.555843 4.3:-14309960 None:intergenic
ATGTGAATGTGATAAGGATG+AGG 0.556397 4.3:+14308070 None:intergenic
ATGTGAATGTGAATGTGATA+AGG 0.557968 4.3:+14308064 None:intergenic
CATGCTGTTGAAAGGCTTGG+AGG 0.559982 4.3:-14309901 MS.gene53441:CDS
GATGAGGTATCATGGACTGA+TGG 0.560341 4.3:+14308177 None:intergenic
GAAATGTTGATGAGGTATCA+TGG 0.573003 4.3:+14308169 None:intergenic
TGTGGAAATTATAACTGTGC+TGG 0.573367 4.3:+14308105 None:intergenic
AGAAATGGAACCTAATTCAT+GGG 0.583302 4.3:+14308211 None:intergenic
TCATGGACTGATGGGCTACA+TGG 0.586690 4.3:+14308186 None:intergenic
TCAGGAGTCCACCTAAGCCT+AGG 0.589432 4.3:+14309943 None:intergenic
ACCTCATCAACATTTCAAGA+TGG 0.592000 4.3:-14308162 MS.gene53441:CDS
ATGAGGGATGCAGGAGACTG+TGG 0.594666 4.3:+14308087 None:intergenic
CATTTCAAGATGGCAAATGG+TGG 0.597539 4.3:-14308152 MS.gene53441:CDS
TGTGAATGTGATAAGGATGA+GGG 0.599304 4.3:+14308071 None:intergenic
AAACTTGACGAGGCTGGACA+AGG 0.608837 4.3:-14308983 MS.gene53441:intron
ATGAGGTATCATGGACTGAT+GGG 0.611244 4.3:+14308178 None:intergenic
TGATTGTTCATGATATCTAA+GGG 0.612865 4.3:+14307735 None:intergenic
TGGTCTCATAGGAGCAAGTG+TGG 0.621257 4.3:+14307788 None:intergenic
GCCATCTTGAAATGTTGATG+AGG 0.640047 4.3:+14308161 None:intergenic
GTTTCCAAAGCTCACCTCAG+CGG 0.640457 4.3:+14307644 None:intergenic
TGGAAACAACACAAGAAGCA+GGG 0.655021 4.3:-14307628 MS.gene53441:CDS
CTTCAACTGATGAATGTGAG+AGG 0.660411 4.3:-14309715 MS.gene53441:CDS
TGTTACCTTAATCCGAAACG+GGG 0.678201 4.3:+14307542 None:intergenic
AACATCACTTAGACCCGCTG+AGG 0.687013 4.3:-14307658 MS.gene53441:CDS
TCAACAGCCATTAATACACA+AGG 0.693154 4.3:-14307867 MS.gene53441:CDS
CCGGAGCAAGAAACTTGACG+AGG 0.718472 4.3:-14308993 MS.gene53441:CDS
GTTACCTTAATCCGAAACGG+GGG 0.718704 4.3:+14307543 None:intergenic
TTGTTCATGATATCTAAGGG+AGG 0.723510 4.3:+14307738 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATATATTAAAATAAAA+GGG - chr4.3:14307938-14307957 MS.gene53441:CDS 0.0%
!! AAATATATTAAAATAAAAGG+GGG - chr4.3:14307940-14307959 MS.gene53441:CDS 10.0%
!!! AGAATTTTTTTTTCATTATA+GGG + chr4.3:14308588-14308607 None:intergenic 10.0%
!!! TAGAATTTTTTTTTCATTAT+AGG + chr4.3:14308589-14308608 None:intergenic 10.0%
!! AATATATTAAAATAAAAGGG+GGG - chr4.3:14307941-14307960 MS.gene53441:intron 15.0%
!! AATGTCAAAATAAACAAAAT+CGG + chr4.3:14309215-14309234 None:intergenic 15.0%
!! AATTGTGAAATTGTTTAATT+TGG - chr4.3:14307682-14307701 MS.gene53441:CDS 15.0%
!! ATATAACAGTGAAAAAAATA+TGG + chr4.3:14308857-14308876 None:intergenic 15.0%
!! TCTAATTAAAAATTTGTACT+GGG - chr4.3:14309137-14309156 MS.gene53441:intron 15.0%
!!! AAAGTTACATTTTTTTACTT+AGG - chr4.3:14309535-14309554 MS.gene53441:intron 15.0%
!!! GTATTTCATAAATCATTTTA+TGG - chr4.3:14308453-14308472 MS.gene53441:intron 15.0%
!!! TTTTCCTTGATTTTTTTTTT+TGG - chr4.3:14308370-14308389 MS.gene53441:intron 15.0%
!! AAACAATTTCACAATTATCT+AGG + chr4.3:14307678-14307697 None:intergenic 20.0%
!! AACAATTTCACAATTATCTA+GGG + chr4.3:14307677-14307696 None:intergenic 20.0%
!! AATAAATACAATAGATTGGT+TGG - chr4.3:14308255-14308274 MS.gene53441:intron 20.0%
!! ATAGAAAAAATATGAAGTGT+TGG - chr4.3:14308299-14308318 MS.gene53441:intron 20.0%
!! ATGCAATAAATACAATAGAT+TGG - chr4.3:14308251-14308270 MS.gene53441:intron 20.0%
!! CACTGTTATATAAAAACAAA+TGG - chr4.3:14308866-14308885 MS.gene53441:intron 20.0%
!! GATTTATAAGACAAAAAAAC+AGG + chr4.3:14308731-14308750 None:intergenic 20.0%
!! GTCTAATTAAAAATTTGTAC+TGG - chr4.3:14309136-14309155 MS.gene53441:intron 20.0%
!! TAGTAATTACATAAAAGAGT+AGG - chr4.3:14309114-14309133 MS.gene53441:intron 20.0%
!! TATATATATACTTACTCTCT+TGG + chr4.3:14307619-14307638 None:intergenic 20.0%
!! TATATCAACACTTAATTTCA+AGG + chr4.3:14308432-14308451 None:intergenic 20.0%
!! TTGAACTATTAAAATAGTTG+TGG + chr4.3:14309917-14309936 None:intergenic 20.0%
!!! AATCAAAGTATTACAATACA+AGG - chr4.3:14309167-14309186 MS.gene53441:intron 20.0%
!!! ATAAATACAATAGATTGGTT+GGG - chr4.3:14308256-14308275 MS.gene53441:intron 20.0%
!!! ATCAAAGTATTACAATACAA+GGG - chr4.3:14309168-14309187 MS.gene53441:intron 20.0%
!!! GTCATCTATTTTAAAATTGA+TGG - chr4.3:14309020-14309039 MS.gene53441:intron 20.0%
!!! TGTAAATTTTTCTTCCAATA+TGG + chr4.3:14309786-14309805 None:intergenic 20.0%
!!! TTATTTTTTTTTCTCTCTAG+TGG + chr4.3:14308223-14308242 None:intergenic 20.0%
!!! TTTATTTTTTTCCATTCAAG+TGG - chr4.3:14309508-14309527 MS.gene53441:intron 20.0%
! AAAGAGAAAGAGAAAAAGTA+TGG + chr4.3:14308647-14308666 None:intergenic 25.0%
! AAATACCGAATGATTTATCT+TGG - chr4.3:14308148-14308167 MS.gene53441:CDS 25.0%
! AAGTAAGTATCATTAGAGTT+GGG + chr4.3:14309066-14309085 None:intergenic 25.0%
! AATTTCTTGAAGAGAAAAGA+TGG - chr4.3:14309733-14309752 MS.gene53441:CDS 25.0%
! ACCACCAAAAAAAAAAATCA+AGG + chr4.3:14308377-14308396 None:intergenic 25.0%
! ATAGTTTACCTTGAATTGAT+GGG + chr4.3:14309462-14309481 None:intergenic 25.0%
! ATGATTGTTCATGATATCTA+AGG + chr4.3:14309763-14309782 None:intergenic 25.0%
! CAATTCCAAGATAAATCATT+CGG + chr4.3:14308156-14308175 None:intergenic 25.0%
! GGTATATAAGACAAAATTTG+AGG - chr4.3:14307835-14307854 MS.gene53441:CDS 25.0%
! GTTGTGTTCATTATTAATGT+TGG - chr4.3:14307724-14307743 MS.gene53441:CDS 25.0%
! TATAGTTTACCTTGAATTGA+TGG + chr4.3:14309463-14309482 None:intergenic 25.0%
! TCTAGTGGTATATTATTCTT+TGG + chr4.3:14308208-14308227 None:intergenic 25.0%
! TGATTGTTCATGATATCTAA+GGG + chr4.3:14309762-14309781 None:intergenic 25.0%
!! AATTTTCATTTCAGATGTAC+CGG - chr4.3:14308482-14308501 MS.gene53441:intron 25.0%
!! AGATCAATTAATTTTTCTGC+AGG - chr4.3:14307985-14308004 MS.gene53441:intron 25.0%
!! ATCTTTTCTCTTCAAGAAAT+TGG + chr4.3:14309734-14309753 None:intergenic 25.0%
!! GATCAATTAATTTTTCTGCA+GGG - chr4.3:14307986-14308005 MS.gene53441:intron 25.0%
!! TAAATACAATAGATTGGTTG+GGG - chr4.3:14308257-14308276 MS.gene53441:intron 25.0%
!! TTCAAATAAGTGACATCTTT+TGG - chr4.3:14308800-14308819 MS.gene53441:intron 25.0%
!!! ATTTTTTTTTTTGGTGGTGA+TGG - chr4.3:14308379-14308398 MS.gene53441:intron 25.0%
!!! TCCTTGATTTTTTTTTTTGG+TGG - chr4.3:14308373-14308392 MS.gene53441:intron 25.0%
AATTGTGCACATGTGTAAAA+CGG - chr4.3:14308989-14309008 MS.gene53441:CDS 30.0%
ACGTGAAACAAAAATATCAG+TGG + chr4.3:14307919-14307938 None:intergenic 30.0%
AGAAATGGAACCTAATTCAT+GGG + chr4.3:14309286-14309305 None:intergenic 30.0%
ATAAAAGAGTGATGAAGATC+AGG + chr4.3:14307572-14307591 None:intergenic 30.0%
ATGTGAATGTGAATGTGATA+AGG + chr4.3:14309433-14309452 None:intergenic 30.0%
CGAATGATTTATCTTGGAAT+TGG - chr4.3:14308154-14308173 MS.gene53441:CDS 30.0%
GAAGTAAGTATCATTAGAGT+TGG + chr4.3:14309067-14309086 None:intergenic 30.0%
GATGATTTAAGTACCATTCT+TGG + chr4.3:14309563-14309582 None:intergenic 30.0%
GGCAGACAAAAAAAAATTCA+AGG + chr4.3:14308567-14308586 None:intergenic 30.0%
GGTACTTAAATCATCAATCT+TGG - chr4.3:14309568-14309587 MS.gene53441:intron 30.0%
GTGTTCATTATTAATGTTGG+TGG - chr4.3:14307727-14307746 MS.gene53441:CDS 30.0%
TATTACAATACAAGGGCTTA+GGG - chr4.3:14309175-14309194 MS.gene53441:intron 30.0%
TCATGAACAATCATCCATAT+TGG - chr4.3:14309769-14309788 MS.gene53441:intron 30.0%
TGATCTTCCTTGTGTATTAA+TGG + chr4.3:14309637-14309656 None:intergenic 30.0%
! TAACTTTAATGCCACTTGAA+TGG + chr4.3:14309522-14309541 None:intergenic 30.0%
!! GAGAAAAAGTATGGTAGTTT+TGG + chr4.3:14308638-14308657 None:intergenic 30.0%
!! TGGACTTTCTTTTTCATGTA+GGG + chr4.3:14308618-14308637 None:intergenic 30.0%
!! TTGGACTTTCTTTTTCATGT+AGG + chr4.3:14308619-14308638 None:intergenic 30.0%
AATTCATTGCTCATGTAGCT+TGG + chr4.3:14309597-14309616 None:intergenic 35.0%
ACCTCATCAACATTTCAAGA+TGG - chr4.3:14309332-14309351 MS.gene53441:intron 35.0%
AGGTGTGTCTAACAAGATTT+AGG + chr4.3:14308547-14308566 None:intergenic 35.0%
ATGTGAATGTGATAAGGATG+AGG + chr4.3:14309427-14309446 None:intergenic 35.0%
ATTCAAAGCCCATCAATTCA+AGG - chr4.3:14309451-14309470 MS.gene53441:intron 35.0%
CAACATTTCAAGATGGCAAA+TGG - chr4.3:14309339-14309358 MS.gene53441:intron 35.0%
GAGAAATGGAACCTAATTCA+TGG + chr4.3:14309287-14309306 None:intergenic 35.0%
GGTGTGTCTAACAAGATTTA+GGG + chr4.3:14308546-14308565 None:intergenic 35.0%
GTATTACAATACAAGGGCTT+AGG - chr4.3:14309174-14309193 MS.gene53441:intron 35.0%
TATTTGAAGAGTGAGAGTAG+CGG + chr4.3:14308788-14308807 None:intergenic 35.0%
TCAACAGCCATTAATACACA+AGG - chr4.3:14309627-14309646 MS.gene53441:intron 35.0%
TGTGAATGTGATAAGGATGA+GGG + chr4.3:14309426-14309445 None:intergenic 35.0%
TGTGGAAATTATAACTGTGC+TGG + chr4.3:14309392-14309411 None:intergenic 35.0%
TGTGTATTAATGGCTGTTGA+TGG + chr4.3:14309627-14309646 None:intergenic 35.0%
TTCTCTTCAAGAAATTGGCT+TGG + chr4.3:14309729-14309748 None:intergenic 35.0%
TTGTTCATGATATCTAAGGG+AGG + chr4.3:14309759-14309778 None:intergenic 35.0%
! ATCAGTTGAAGCACCAATTT+GGG + chr4.3:14307770-14307789 None:intergenic 35.0%
! CTTCCCAGCAATTTCATTTT+GGG - chr4.3:14308697-14308716 MS.gene53441:intron 35.0%
! CTTTTATGCATGCTGTTGAA+AGG - chr4.3:14307585-14307604 MS.gene53441:CDS 35.0%
! GAAATGTTGATGAGGTATCA+TGG + chr4.3:14309328-14309347 None:intergenic 35.0%
! TTCCCAGCAATTTCATTTTG+GGG - chr4.3:14308698-14308717 MS.gene53441:intron 35.0%
! TTTTACTTAGGCACCAAGAA+TGG - chr4.3:14309547-14309566 MS.gene53441:intron 35.0%
!!! TGGTGGTGATGGTATTTTTT+TGG - chr4.3:14308390-14308409 MS.gene53441:intron 35.0%
AACCTACAAACATTATCCCC+CGG + chr4.3:14308025-14308044 None:intergenic 40.0%
CACAGATCAACCCATGAATT+AGG - chr4.3:14309273-14309292 MS.gene53441:intron 40.0%
CATTTCAAGATGGCAAATGG+TGG - chr4.3:14309342-14309361 MS.gene53441:intron 40.0%
CCAAAATGAAATTGCTGGGA+AGG + chr4.3:14308699-14308718 None:intergenic 40.0%
CTTCAACTGATGAATGTGAG+AGG - chr4.3:14307779-14307798 MS.gene53441:CDS 40.0%
GCCATCTTGAAATGTTGATG+AGG + chr4.3:14309336-14309355 None:intergenic 40.0%
TGGAAACAACACAAGAAGCA+GGG - chr4.3:14309866-14309885 MS.gene53441:intron 40.0%
TTGAATTTGATCCCCCGTTT+CGG - chr4.3:14309940-14309959 MS.gene53441:CDS 40.0%
TTGGAAACAACACAAGAAGC+AGG - chr4.3:14309865-14309884 MS.gene53441:intron 40.0%
! ATGAGGTATCATGGACTGAT+GGG + chr4.3:14309319-14309338 None:intergenic 40.0%
! CATCAGTTGAAGCACCAATT+TGG + chr4.3:14307771-14307790 None:intergenic 40.0%
! CCTTCCCAGCAATTTCATTT+TGG - chr4.3:14308696-14308715 MS.gene53441:intron 40.0%
! TATCCTTAACTTGAAGCTGC+TGG + chr4.3:14309674-14309693 None:intergenic 40.0%
! TCCCAGCAATTTCATTTTGG+GGG - chr4.3:14308699-14308718 MS.gene53441:intron 40.0%
!! AAGCTGCTGGTTCAAAGTTA+TGG + chr4.3:14309661-14309680 None:intergenic 40.0%
!! AGAAATTGGCTTGGTCTCAT+AGG + chr4.3:14309720-14309739 None:intergenic 40.0%
ATGCATGCTGTTGAAAGGCT+TGG - chr4.3:14307590-14307609 MS.gene53441:CDS 45.0%
ATTCCCCGCAAAACAGGTTT+GGG - chr4.3:14308667-14308686 MS.gene53441:intron 45.0%
CCCCCCAAAATGAAATTGCT+GGG + chr4.3:14308703-14308722 None:intergenic 45.0%
CTCTTTATTCCCCGCAAAAC+AGG - chr4.3:14308661-14308680 MS.gene53441:intron 45.0%
TATTCCCCGCAAAACAGGTT+TGG - chr4.3:14308666-14308685 MS.gene53441:intron 45.0%
TCATGTCAAGAGTCACTTGC+AGG - chr4.3:14307814-14307833 MS.gene53441:CDS 45.0%
TCCCCCCAAAATGAAATTGC+TGG + chr4.3:14308704-14308723 None:intergenic 45.0%
! ATGGGCTACATGGTGAGAAA+TGG + chr4.3:14309301-14309320 None:intergenic 45.0%
! CCCAGCAATTTCATTTTGGG+GGG - chr4.3:14308700-14308719 MS.gene53441:intron 45.0%
! GAACCAGCAGCTTCAAGTTA+AGG - chr4.3:14309668-14309687 MS.gene53441:intron 45.0%
! GATGAGGTATCATGGACTGA+TGG + chr4.3:14309320-14309339 None:intergenic 45.0%
!! GCAATTTCATTTTGGGGGGA+AGG - chr4.3:14308704-14308723 MS.gene53441:intron 45.0%
!! TCATGGGTTGATCTGTGTGT+TGG + chr4.3:14309270-14309289 None:intergenic 45.0%
!! TTAATGTTGGTGGTGCATGC+AGG - chr4.3:14307737-14307756 MS.gene53441:CDS 45.0%
!! AAAATATATTAAAATAAAAG+GGG - chr4.3:14307939-14307958 MS.gene53441:CDS 5.0%
!! GTAAAATATATTAAAATAAA+AGG - chr4.3:14307937-14307956 MS.gene53441:CDS 5.0%
!!! ATTTTTTTTATTGATTAATT+TGG + chr4.3:14308132-14308151 None:intergenic 5.0%
AAACTTGACGAGGCTGGACA+AGG - chr4.3:14308511-14308530 MS.gene53441:intron 50.0%
AACATCACTTAGACCCGCTG+AGG - chr4.3:14309836-14309855 MS.gene53441:intron 50.0%
AGCAAGAAACTTGACGAGGC+TGG - chr4.3:14308505-14308524 MS.gene53441:intron 50.0%
CACCGGGGGATAATGTTTGT+AGG - chr4.3:14308020-14308039 MS.gene53441:intron 50.0%
CATGCTGTTGAAAGGCTTGG+AGG - chr4.3:14307593-14307612 MS.gene53441:CDS 50.0%
CCTCGTCAAGTTTCTTGCTC+CGG + chr4.3:14308504-14308523 None:intergenic 50.0%
CTACAAACATTATCCCCCGG+TGG + chr4.3:14308022-14308041 None:intergenic 50.0%
GTGATAAGGATGAGGGATGC+AGG + chr4.3:14309419-14309438 None:intergenic 50.0%
GTTTCCAAAGCTCACCTCAG+CGG + chr4.3:14309853-14309872 None:intergenic 50.0%
TGCAGGAGCAACACCCAAAT+TGG - chr4.3:14307754-14307773 MS.gene53441:CDS 50.0%
TGGTCTCATAGGAGCAAGTG+TGG + chr4.3:14309709-14309728 None:intergenic 50.0%
TTCCCCGCAAAACAGGTTTG+GGG - chr4.3:14308668-14308687 MS.gene53441:intron 50.0%
TTTCCAAAGCTCACCTCAGC+GGG + chr4.3:14309852-14309871 None:intergenic 50.0%
!! TCATGGACTGATGGGCTACA+TGG + chr4.3:14309311-14309330 None:intergenic 50.0%
ACATTATCCCCCGGTGGACA+CGG + chr4.3:14308016-14308035 None:intergenic 55.0%
ATGAGGGATGCAGGAGACTG+TGG + chr4.3:14309410-14309429 None:intergenic 55.0%
CCGGAGCAAGAAACTTGACG+AGG - chr4.3:14308501-14308520 MS.gene53441:intron 55.0%
TCAGGAGTCCACCTAAGCCT+AGG + chr4.3:14307554-14307573 None:intergenic 55.0%
TCCCCGCAAAACAGGTTTGG+GGG - chr4.3:14308669-14308688 MS.gene53441:intron 55.0%
TGACGAGGCTGGACAAGGTA+AGG - chr4.3:14308516-14308535 MS.gene53441:intron 55.0%
! ACCCCCCAAACCTGTTTTGC+GGG + chr4.3:14308674-14308693 None:intergenic 55.0%
! CACCCCCCAAACCTGTTTTG+CGG + chr4.3:14308675-14308694 None:intergenic 55.0%
AGACCCGCTGAGGTGAGCTT+TGG - chr4.3:14309846-14309865 MS.gene53441:intron 60.0%
CCCCGCAAAACAGGTTTGGG+GGG - chr4.3:14308670-14308689 MS.gene53441:intron 60.0%
! CCCCCCAAACCTGTTTTGCG+GGG + chr4.3:14308673-14308692 None:intergenic 60.0%
AGGGTGATCCGTGTCCACCG+GGG - chr4.3:14308005-14308024 MS.gene53441:intron 65.0%
CAGGGTGATCCGTGTCCACC+GGG - chr4.3:14308004-14308023 MS.gene53441:intron 65.0%
GCAGGGTGATCCGTGTCCAC+CGG - chr4.3:14308003-14308022 MS.gene53441:intron 65.0%
GGGTGATCCGTGTCCACCGG+GGG - chr4.3:14308006-14308025 MS.gene53441:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 14307548 14309968 14307548 ID=MS.gene53441
chr4.3 mRNA 14307548 14309968 14307548 ID=MS.gene53441.t1;Parent=MS.gene53441
chr4.3 exon 14309893 14309968 14309893 ID=MS.gene53441.t1.exon1;Parent=MS.gene53441.t1
chr4.3 CDS 14309893 14309968 14309893 ID=cds.MS.gene53441.t1;Parent=MS.gene53441.t1
chr4.3 exon 14309681 14309757 14309681 ID=MS.gene53441.t1.exon2;Parent=MS.gene53441.t1
chr4.3 CDS 14309681 14309757 14309681 ID=cds.MS.gene53441.t1;Parent=MS.gene53441.t1
chr4.3 exon 14308984 14309020 14308984 ID=MS.gene53441.t1.exon3;Parent=MS.gene53441.t1
chr4.3 CDS 14308984 14309020 14308984 ID=cds.MS.gene53441.t1;Parent=MS.gene53441.t1
chr4.3 exon 14308044 14308257 14308044 ID=MS.gene53441.t1.exon4;Parent=MS.gene53441.t1
chr4.3 CDS 14308044 14308257 14308044 ID=cds.MS.gene53441.t1;Parent=MS.gene53441.t1
chr4.3 exon 14307548 14307959 14307548 ID=MS.gene53441.t1.exon5;Parent=MS.gene53441.t1
chr4.3 CDS 14307548 14307959 14307548 ID=cds.MS.gene53441.t1;Parent=MS.gene53441.t1
Gene Sequence

>MS.gene53441

ATGCCTAGGCTTAGGTGGACTCCTGATCTTCATCACTCTTTTATGCATGCTGTTGAAAGGCTTGGAGGCCAAGAGAGTAAGTATATATATATTTTTTGTCTTAATTCTTTAGAAGAAAAAACTATGCCCTAGATAATTGTGAAATTGTTTAATTTGGTTGAAGTTGAAAACTTTATGTTGTGTTCATTATTAATGTTGGTGGTGCATGCAGGAGCAACACCCAAATTGGTGCTTCAACTGATGAATGTGAGAGGACTCAGCATTGCTCATGTCAAGAGTCACTTGCAGGTATATAAGACAAAATTTGAGGTAGATATATATGTTTCTTAAAAGAAAAAAAACAAAACTGTACAACTTCAATCTTATAGCCACTGATATTTTTGTTTCACGTAAAATATATTAAAATAAAAGGGGGGAATAATTGAGAAACATATATTAGATCAATTAATTTTTCTGCAGGGTGATCCGTGTCCACCGGGGGATAATGTTTGTAGGTTTTTTTAATTTGATTTTTGATGTTCTTTATGATAAGTTTTATAAAAAAATAAAAATCATTTATATATTCAAACAATTTGTACATTCCAAATTAATCAATAAAAAAAATACCGAATGATTTATCTTGGAATTGGATATTTTTTTAAAAATGATTTTTAGACACCAAAGAATAATATACCACTAGAGAGAAAAAAAAATAAAGATAGCGATGCAATAAATACAATAGATTGGTTGGGGATGTGATATGAAGAAAGAGATAGAAAAAATATGAAGTGTTGGATGAATGTAAAAAAAGTAAAAGTGTTTAAATATAATTGCTCTTTTTTTTTTTCCTTGATTTTTTTTTTTGGTGGTGATGGTATTTTTTTGGTAGTGATTTTATTTTTCCTTGAAATTAAGTGTTGATATATGTATTTCATAAATCATTTTATGGCTATGCAATTTTCATTTCAGATGTACCGGAGCAAGAAACTTGACGAGGCTGGACAAGGTAAGGATTGCCCTAAATCTTGTTAGACACACCTTGAATTTTTTTTTGTCTGCCCTATAATGAAAAAAAAATTCTAAATCTGCCCTACATGAAAAAGAAAGTCCAAAACTACCATACTTTTTCTCTTTCTCTTTATTCCCCGCAAAACAGGTTTGGGGGGTGTCCTTCCCAGCAATTTCATTTTGGGGGGAAGGTCCTGTTTTTTTGTCTTATAAATCTACTACATGCTTTGAGAATATACAATCATTCAAACCGCTACTCTCACTCTTCAAATAAGTGACATCTTTTGGCTATGTGTACTATTCATATAATATATTTTGACCATATTTTTTTCACTGTTATATAAAAACAAATGGTGTCATATAAGATGTTGTGTGAATTTGTCTCTATCAACATTTTAAAAACATCAAATTTTATATTTTTTTTAATTACATAATTAAAAATATTAAAGATTAAAATTGTGCACATGTGTAAAACGGTCAATGATGTCATCTATTTTAAAATTGATGGAGTAAATGTTATGTTAGATTCCCAACTCTAATGATACTTACTTCTGTCTTTTAAAAAAAAACATTATTTTTTAGTAATTACATAAAAGAGTAGGTCTAATTAAAAATTTGTACTGGGTGTTCAAAATCAAAGTATTACAATACAAGGGCTTAGGGTTAGTTTACTTTAGCCGATTTTGTTTATTTTGACATTTATTCATATTTATTTACTTTGCAGTACTAAGTCCAACACACAGATCAACCCATGAATTAGGTTCCATTTCTCACCATGTAGCCCATCAGTCCATGATACCTCATCAACATTTCAAGATGGCAAATGGTGGAATTGTTCTAGAAACTAATTACAACCAGCACAGTTATAATTTCCACAGTCTCCTGCATCCCTCATCCTTATCACATTCACATTCACATTCAAAGCCCATCAATTCAAGGTAAACTATATATGCATACATTCTTATTTATTTATTTTATTTTTTTCCATTCAAGTGGCATTAAAGTTACATTTTTTTACTTAGGCACCAAGAATGGTACTTAAATCATCAATCTTGGAGACCAAGCTACATGAGCAATGAATTAGTTATGCCATCAACAGCCATTAATACACAAGGAAGATCATCCATAACTTTGAACCAGCAGCTTCAAGTTAAGGATACTACAGTCACTACCACACTTGCTCCTATGAGACCAAGCCAATTTCTTGAAGAGAAAAGATGGCCTCCCTTAGATATCATGAACAATCATCCATATTGGAAGAAAAATTTACATGATAATTTTTCTAATAATACTTGTGATAGAACATCACTTAGACCCGCTGAGGTGAGCTTTGGAAACAACACAAGAAGCAGGGAAAACATATCAAACATCAATAATGACCACAACTATTTTAATAGTTCAAAGATTGAATTTGATCCCCCGTTTCGGATTAAG
Protein sequence

>MS.gene53441.t1

MPRLRWTPDLHHSFMHAVERLGGQERATPKLVLQLMNVRGLSIAHVKSHLQMYRSKKLDEAGQVLSPTHRSTHELGSISHHVAHQSMIPHQHFKMANGGIVLETNYNQHSYNFHSLLHPSSLSHSHSHSKPINSRHQEWYLNHQSWRPSYMSNELVMPSTAINTQGRSSITLNQQLQVKDTTVTTTLAPMRPSQFLEEKRWPPLDIMNNHPYWKKNLHDNFSNNTCDRTSLRPAEVSFGNNTRSRENISNINNDHNYFNSSKIEFDPPFRIK