AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53592

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53592.t1 MTR_1g046340 61.254 351 115 4 2 351 5 335 1.81e-135 389
MS.gene53592.t1 MTR_1g046200 91.716 169 13 1 184 351 156 324 3.20e-111 327
MS.gene53592.t1 MTR_1g046320 72.674 172 45 1 179 350 164 333 2.54e-87 266
MS.gene53592.t1 MTR_3g088515 52.823 248 98 5 2 246 16 247 7.59e-58 188
MS.gene53592.t1 MTR_1g021435 26.380 163 114 3 9 167 22 182 3.20e-14 73.6
MS.gene53592.t1 MTR_1g021400 25.352 142 102 2 13 154 36 173 9.50e-12 65.9
MS.gene53592.t1 MTR_1g021330 27.692 130 90 2 9 136 26 153 3.25e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53592.t1 AT3G06160 23.256 344 222 6 6 349 8 309 3.76e-21 92.8
MS.gene53592.t1 AT3G06160 23.256 344 222 6 6 349 8 309 3.76e-21 92.8
MS.gene53592.t1 AT3G06160 23.256 344 222 6 6 349 8 309 3.76e-21 92.8
MS.gene53592.t1 AT3G06160 23.256 344 222 6 6 349 23 324 4.16e-21 92.8
MS.gene53592.t1 AT3G06160 23.256 344 222 6 6 349 67 368 8.98e-21 92.8
MS.gene53592.t1 AT5G57720 21.972 355 202 13 5 349 10 299 2.30e-14 73.2
MS.gene53592.t1 AT3G18960 37.624 101 59 3 8 107 30 127 7.75e-12 64.3
MS.gene53592.t1 AT3G18960 37.624 101 59 3 8 107 30 127 9.03e-12 63.9
MS.gene53592.t1 AT4G01580 36.364 99 59 3 8 105 30 125 2.92e-11 62.4
MS.gene53592.t1 AT5G66980 23.164 354 223 12 1 348 4 314 7.84e-11 63.2

Find 58 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATGAAGTGTGGATTTC+CGG 0.187944 1.2:+29120267 None:intergenic
AAATCCTTGATAACATTCTT+TGG 0.259575 1.2:+29119928 None:intergenic
TGTTAAGGGTGCACATTGTT+AGG 0.310846 1.2:-29119527 MS.gene53592:CDS
TGCTGCTGATGATGATATTA+TGG 0.325258 1.2:-29120627 MS.gene53592:CDS
GCATCAATTTCGTCGTCTTC+AGG 0.326868 1.2:+29120307 None:intergenic
CTTACCAGCTTTGCACTTGC+AGG 0.327904 1.2:+29120466 None:intergenic
AAGAATTGTACCAATTCATT+AGG 0.342988 1.2:+29120226 None:intergenic
TAGAGACGAAAATTCTTTAG+AGG 0.344242 1.2:-29120849 MS.gene53592:CDS
AGATCATGATGATGATATTA+TGG 0.349539 1.2:-29120594 MS.gene53592:CDS
ATCAAGTTCACAAAAGCATT+AGG 0.355825 1.2:+29120992 None:intergenic
TGAACTTGATGCGTTCAAAA+AGG 0.358189 1.2:-29120979 MS.gene53592:CDS
CTCTTCCTCTACGATCTCTT+AGG 0.378241 1.2:+29120936 None:intergenic
TTGTACCAATTCATTAGGTC+TGG 0.410085 1.2:+29120231 None:intergenic
GCAATGAAGTGTGGATTTCC+GGG 0.417877 1.2:+29120268 None:intergenic
ATATTGAAAATAACATATTC+AGG 0.423216 1.2:-29120802 MS.gene53592:CDS
ATATGTGTTCTGCTTATTTG+TGG 0.425629 1.2:+29119693 None:intergenic
TCAAGTTCACAAAAGCATTA+GGG 0.428525 1.2:+29120993 None:intergenic
TCATTCCAGACCTAATGAAT+TGG 0.430343 1.2:-29120236 MS.gene53592:intron
CCTTGCAGTGGCAACATTCA+AGG 0.435374 1.2:+29119878 None:intergenic
CATGGAGGAAAATAACAATA+TGG 0.446533 1.2:-29120729 MS.gene53592:CDS
CATCTCTCCACGTACGTACT+TGG 0.449196 1.2:+29119658 None:intergenic
CATGTCAAAGCTCGTCGTAT+TGG 0.455772 1.2:-29120908 MS.gene53592:CDS
TATTCCAAAGAATGTTATCA+AGG 0.458986 1.2:-29119932 MS.gene53592:CDS
GATGGTCGAGTACGTGTGCT+AGG 0.481978 1.2:-29119639 MS.gene53592:CDS
TTGTGGCAAGATGTGATGAT+AGG 0.486090 1.2:+29119710 None:intergenic
GAGATCGTAGAGGAAGAGAT+TGG 0.486399 1.2:-29120931 MS.gene53592:CDS
GAAGAAATCTGGGTAATCCT+TGG 0.489215 1.2:+29121229 None:intergenic
CAATATGGAGGATGAGGAAG+AGG 0.500994 1.2:-29120714 MS.gene53592:CDS
GTCGAGTACGTGTGCTAGGG+TGG 0.503803 1.2:-29119635 MS.gene53592:CDS
AGAACCTGCAAGTGCAAAGC+TGG 0.504053 1.2:-29120470 MS.gene53592:intron
AAATAACAATATGGAGGATG+AGG 0.505646 1.2:-29120720 MS.gene53592:CDS
AGAAAAGCACACTGAAAGAA+TGG 0.515189 1.2:-29121192 MS.gene53592:intron
ATCAATGTGTGAAAAGAAGA+AGG 0.520397 1.2:-29120759 MS.gene53592:CDS
AAGGGTGCACATTGTTAGGA+AGG 0.552584 1.2:-29119523 MS.gene53592:CDS
GATGAGGAAGAGGACGGTGA+TGG 0.556360 1.2:-29120704 MS.gene53592:CDS
CGAGAATGAAAGAATGTACA+AGG 0.558420 1.2:-29120522 MS.gene53592:CDS
TGATGATGATATTATGGCAG+AGG 0.566025 1.2:-29120588 MS.gene53592:CDS
AGACGAAAATTCTTTAGAGG+AGG 0.570120 1.2:-29120846 MS.gene53592:CDS
GAGATTGTGCTGCGTGAAGA+TGG 0.578226 1.2:-29119561 MS.gene53592:CDS
CTTGCAGTGGCAACATTCAA+GGG 0.578433 1.2:+29119879 None:intergenic
CAAGTACGTACGTGGAGAGA+TGG 0.582116 1.2:-29119657 MS.gene53592:CDS
CCTTGAATGTTGCCACTGCA+AGG 0.583961 1.2:-29119878 MS.gene53592:intron
AAAGAAGAAGGTAAGTGACA+TGG 0.601455 1.2:-29120747 MS.gene53592:CDS
AATTGTAACTAAGATGGCCA+AGG 0.605912 1.2:-29121246 None:intergenic
GAAGAAGGTAAGTGACATGG+AGG 0.606864 1.2:-29120744 MS.gene53592:CDS
CATGATACCGATGATGATGA+TGG 0.612164 1.2:-29120557 MS.gene53592:CDS
ATGGTCGAGTACGTGTGCTA+GGG 0.616130 1.2:-29119638 MS.gene53592:CDS
TCGAGTACGTGTGCTAGGGT+GGG 0.622095 1.2:-29119634 MS.gene53592:CDS
GTCATCACCATCATCATCAT+CGG 0.626181 1.2:+29120550 None:intergenic
TGATGATGATATTATGGCAG+AGG 0.627493 1.2:-29120621 MS.gene53592:CDS
AATTATACTATACCTTGCAG+TGG 0.634623 1.2:+29119866 None:intergenic
GGAGGAAAATAACAATATGG+AGG 0.639547 1.2:-29120726 MS.gene53592:CDS
AGAGAGGCCAAGTACGTACG+TGG 0.642453 1.2:-29119665 MS.gene53592:CDS
GCTGAAATGTATATTCAACC+CGG 0.655563 1.2:-29120286 MS.gene53592:CDS
ATGGAGGATGAGGAAGAGGA+CGG 0.656729 1.2:-29120710 MS.gene53592:CDS
AGAACACATATCGAAAAGAG+AGG 0.666945 1.2:-29119681 MS.gene53592:CDS
TGATGATGATGACGATGACG+AGG 0.734080 1.2:-29120678 MS.gene53592:CDS
TTCATCCTAAGAGATCGTAG+AGG 0.754677 1.2:-29120941 MS.gene53592:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTGAAAATAACATATTC+AGG - chr1.2:29119949-29119968 MS.gene53592:intron 15.0%
!! TGTCAAAAAAAAAAAAGTAA+TGG + chr1.2:29120556-29120575 None:intergenic 15.0%
!!! TTACTTTTTTTTTTTTGACA+TGG - chr1.2:29120556-29120575 MS.gene53592:CDS 15.0%
!! CAAAAAGGAATAAAAAACAT+TGG + chr1.2:29119620-29119639 None:intergenic 20.0%
!! CTAAATCAAAATAAATCATC+AGG + chr1.2:29120392-29120411 None:intergenic 20.0%
!! TCATAATAGTGAAAAATGAA+AGG + chr1.2:29120921-29120940 None:intergenic 20.0%
!! TTTCAATATGTGTAAAAACA+AGG + chr1.2:29119938-29119957 None:intergenic 20.0%
!!! AAAAAACTTTGAAGAAATCT+GGG + chr1.2:29119535-29119554 None:intergenic 20.0%
!!! CAAAATAGTAGTTTTATCTT+CGG + chr1.2:29120324-29120343 None:intergenic 20.0%
!!! GATGATTTATTTTGATTTAG+CGG - chr1.2:29120392-29120411 MS.gene53592:intron 20.0%
! AAGAATTGTACCAATTCATT+AGG + chr1.2:29120528-29120547 None:intergenic 25.0%
! AATAAAGAGAATAACTAGAC+AGG + chr1.2:29120694-29120713 None:intergenic 25.0%
! ATCAACACATTAAACAAAAG+TGG + chr1.2:29120952-29120971 None:intergenic 25.0%
! ATGGAAAAATCGAAATGTTA+AGG - chr1.2:29121209-29121228 MS.gene53592:CDS 25.0%
! GAAAAATGAAAGGTAAAGAT+TGG + chr1.2:29120911-29120930 None:intergenic 25.0%
! TATTCCAAAGAATGTTATCA+AGG - chr1.2:29120819-29120838 MS.gene53592:CDS 25.0%
! TGGAAAAATCGAAATGTTAA+GGG - chr1.2:29121210-29121229 MS.gene53592:CDS 25.0%
!! AAATCCTTGATAACATTCTT+TGG + chr1.2:29120826-29120845 None:intergenic 25.0%
!! AGATCATGATGATGATATTA+TGG - chr1.2:29120157-29120176 MS.gene53592:intron 25.0%
!! ATATATTTTGCAATGAAGTG+TGG + chr1.2:29120495-29120514 None:intergenic 25.0%
!! CATTTTTCACTATTATGAAG+TGG - chr1.2:29120923-29120942 MS.gene53592:CDS 25.0%
!! CTTTTTGATAAAAACCAGTT+TGG - chr1.2:29119633-29119652 MS.gene53592:CDS 25.0%
!! GAAAAAACTTTGAAGAAATC+TGG + chr1.2:29119536-29119555 None:intergenic 25.0%
!!! CAAACTGGTTTTTATCAAAA+AGG + chr1.2:29119635-29119654 None:intergenic 25.0%
AAAAAACATTGGAGAAAGCA+TGG + chr1.2:29119609-29119628 None:intergenic 30.0%
AAATAACAATATGGAGGATG+AGG - chr1.2:29120031-29120050 MS.gene53592:intron 30.0%
AATTATACTATACCTTGCAG+TGG + chr1.2:29120888-29120907 None:intergenic 30.0%
ATATGTGTTCTGCTTATTTG+TGG + chr1.2:29121061-29121080 None:intergenic 30.0%
ATCAATGTGTGAAAAGAAGA+AGG - chr1.2:29119992-29120011 MS.gene53592:intron 30.0%
CAAGTAAAATTGCAACAACT+TGG + chr1.2:29120727-29120746 None:intergenic 30.0%
CATGGAGGAAAATAACAATA+TGG - chr1.2:29120022-29120041 MS.gene53592:intron 30.0%
TAGAGACGAAAATTCTTTAG+AGG - chr1.2:29119902-29119921 MS.gene53592:CDS 30.0%
TATTTCTTTGCATACCAAAC+TGG + chr1.2:29119650-29119669 None:intergenic 30.0%
TTCAAAAAGGAAGGTCATAA+AGG - chr1.2:29119785-29119804 MS.gene53592:intron 30.0%
!! ATCAAGTTCACAAAAGCATT+AGG + chr1.2:29119762-29119781 None:intergenic 30.0%
!! TCAAGTTCACAAAAGCATTA+GGG + chr1.2:29119761-29119780 None:intergenic 30.0%
!!! AAGTTGCATTCATCCTTTTT+TGG - chr1.2:29120984-29121003 MS.gene53592:CDS 30.0%
!!! ATTTTGATTTAGCGGACATT+GGG - chr1.2:29120400-29120419 MS.gene53592:intron 30.0%
!!! TATTTTGATTTAGCGGACAT+TGG - chr1.2:29120399-29120418 MS.gene53592:intron 30.0%
AAAGAAGAAGGTAAGTGACA+TGG - chr1.2:29120004-29120023 MS.gene53592:intron 35.0%
ACTTCAAATGTGAAGAGTGA+TGG + chr1.2:29120798-29120817 None:intergenic 35.0%
AGAACACATATCGAAAAGAG+AGG - chr1.2:29121070-29121089 MS.gene53592:intron 35.0%
CGAGAATGAAAGAATGTACA+AGG - chr1.2:29120229-29120248 MS.gene53592:intron 35.0%
GATAAGAAATGACAAGTCGA+AGG + chr1.2:29120650-29120669 None:intergenic 35.0%
GCTGAAATGTATATTCAACC+CGG - chr1.2:29120465-29120484 MS.gene53592:intron 35.0%
GGAGGAAAATAACAATATGG+AGG - chr1.2:29120025-29120044 MS.gene53592:intron 35.0%
TCATTCCAGACCTAATGAAT+TGG - chr1.2:29120515-29120534 MS.gene53592:CDS 35.0%
TGAACTTGATGCGTTCAAAA+AGG - chr1.2:29119772-29119791 MS.gene53592:intron 35.0%
TGTCCTGTCATAACCAAAAA+AGG + chr1.2:29121000-29121019 None:intergenic 35.0%
TTGTACCAATTCATTAGGTC+TGG + chr1.2:29120523-29120542 None:intergenic 35.0%
! AGAAAAGCACACTGAAAGAA+TGG - chr1.2:29119559-29119578 MS.gene53592:CDS 35.0%
! AGACGAAAATTCTTTAGAGG+AGG - chr1.2:29119905-29119924 MS.gene53592:CDS 35.0%
! TGATGATGATATTATGGCAG+AGG - chr1.2:29120130-29120149 MS.gene53592:intron 35.0%
! TGCTGCTGATGATGATATTA+TGG - chr1.2:29120124-29120143 MS.gene53592:intron 35.0%
!!! AGCTTTATTTTGACGATGGT+TGG - chr1.2:29119871-29119890 MS.gene53592:intron 35.0%
!!! CATCCTTTTTTGGTTATGAC+AGG - chr1.2:29120994-29121013 MS.gene53592:CDS 35.0%
!!! GATGAGCTTTATTTTGACGA+TGG - chr1.2:29119867-29119886 MS.gene53592:intron 35.0%
ATTTGCTTAACCCTAGGTAG+AGG - chr1.2:29120606-29120625 MS.gene53592:CDS 40.0%
CTTGATGCGTTCAAAAAGGA+AGG - chr1.2:29119776-29119795 MS.gene53592:intron 40.0%
GAAGAAATCTGGGTAATCCT+TGG + chr1.2:29119525-29119544 None:intergenic 40.0%
GTCATCACCATCATCATCAT+CGG + chr1.2:29120204-29120223 None:intergenic 40.0%
TAGACAGGCATAGAGTACAA+AGG + chr1.2:29120679-29120698 None:intergenic 40.0%
TAGTCCATTTGCTTAACCCT+AGG - chr1.2:29120600-29120619 MS.gene53592:CDS 40.0%
TCTACCTAGGGTTAAGCAAA+TGG + chr1.2:29120607-29120626 None:intergenic 40.0%
TGCAATGAAGTGTGGATTTC+CGG + chr1.2:29120487-29120506 None:intergenic 40.0%
TTCATCCTAAGAGATCGTAG+AGG - chr1.2:29119810-29119829 MS.gene53592:intron 40.0%
TTGTGGCAAGATGTGATGAT+AGG + chr1.2:29121044-29121063 None:intergenic 40.0%
! CATGATACCGATGATGATGA+TGG - chr1.2:29120194-29120213 MS.gene53592:intron 40.0%
! TGACATGTTTTGTGAAGCAG+AGG + chr1.2:29120852-29120871 None:intergenic 40.0%
! TGTTAAGGGTGCACATTGTT+AGG - chr1.2:29121224-29121243 MS.gene53592:CDS 40.0%
CAATATGGAGGATGAGGAAG+AGG - chr1.2:29120037-29120056 MS.gene53592:intron 45.0%
CATGTCAAAGCTCGTCGTAT+TGG - chr1.2:29119843-29119862 MS.gene53592:intron 45.0%
CTCTTCCTCTACGATCTCTT+AGG + chr1.2:29119818-29119837 None:intergenic 45.0%
CTTGCAGTGGCAACATTCAA+GGG + chr1.2:29120875-29120894 None:intergenic 45.0%
GAAGAAGGTAAGTGACATGG+AGG - chr1.2:29120007-29120026 MS.gene53592:intron 45.0%
GAGATCGTAGAGGAAGAGAT+TGG - chr1.2:29119820-29119839 MS.gene53592:intron 45.0%
GCAATGAAGTGTGGATTTCC+GGG + chr1.2:29120486-29120505 None:intergenic 45.0%
GCATCAATTTCGTCGTCTTC+AGG + chr1.2:29120447-29120466 None:intergenic 45.0%
TCGACTAACTCCTCTACCTA+GGG + chr1.2:29120619-29120638 None:intergenic 45.0%
TTCGACTAACTCCTCTACCT+AGG + chr1.2:29120620-29120639 None:intergenic 45.0%
! AAGGGTGCACATTGTTAGGA+AGG - chr1.2:29121228-29121247 MS.gene53592:CDS 45.0%
! TGATGATGATGACGATGACG+AGG - chr1.2:29120073-29120092 MS.gene53592:intron 45.0%
AGAACCTGCAAGTGCAAAGC+TGG - chr1.2:29120281-29120300 MS.gene53592:CDS 50.0%
ATGGAGGATGAGGAAGAGGA+CGG - chr1.2:29120041-29120060 MS.gene53592:intron 50.0%
ATGGTCGAGTACGTGTGCTA+GGG - chr1.2:29121113-29121132 MS.gene53592:intron 50.0%
CAAGTACGTACGTGGAGAGA+TGG - chr1.2:29121094-29121113 MS.gene53592:intron 50.0%
CATCTCTCCACGTACGTACT+TGG + chr1.2:29121096-29121115 None:intergenic 50.0%
CCTTGAATGTTGCCACTGCA+AGG - chr1.2:29120873-29120892 MS.gene53592:CDS 50.0%
CCTTGCAGTGGCAACATTCA+AGG + chr1.2:29120876-29120895 None:intergenic 50.0%
CTTACCAGCTTTGCACTTGC+AGG + chr1.2:29120288-29120307 None:intergenic 50.0%
GAGATTGTGCTGCGTGAAGA+TGG - chr1.2:29121190-29121209 MS.gene53592:intron 50.0%
AGAGAGGCCAAGTACGTACG+TGG - chr1.2:29121086-29121105 MS.gene53592:intron 55.0%
GATGAGGAAGAGGACGGTGA+TGG - chr1.2:29120047-29120066 MS.gene53592:intron 55.0%
GATGGTCGAGTACGTGTGCT+AGG - chr1.2:29121112-29121131 MS.gene53592:intron 55.0%
TCGAGTACGTGTGCTAGGGT+GGG - chr1.2:29121117-29121136 MS.gene53592:intron 55.0%
GTCGAGTACGTGTGCTAGGG+TGG - chr1.2:29121116-29121135 MS.gene53592:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 29119518 29121255 29119518 ID=MS.gene53592
chr1.2 mRNA 29119518 29121255 29119518 ID=MS.gene53592.t1;Parent=MS.gene53592
chr1.2 exon 29121193 29121255 29121193 ID=MS.gene53592.t1.exon1;Parent=MS.gene53592.t1
chr1.2 CDS 29121193 29121255 29121193 ID=cds.MS.gene53592.t1;Parent=MS.gene53592.t1
chr1.2 exon 29120471 29121020 29120471 ID=MS.gene53592.t1.exon2;Parent=MS.gene53592.t1
chr1.2 CDS 29120471 29121020 29120471 ID=cds.MS.gene53592.t1;Parent=MS.gene53592.t1
chr1.2 exon 29120237 29120361 29120237 ID=MS.gene53592.t1.exon3;Parent=MS.gene53592.t1
chr1.2 CDS 29120237 29120361 29120237 ID=cds.MS.gene53592.t1;Parent=MS.gene53592.t1
chr1.2 exon 29119879 29119956 29119879 ID=MS.gene53592.t1.exon4;Parent=MS.gene53592.t1
chr1.2 CDS 29119879 29119956 29119879 ID=cds.MS.gene53592.t1;Parent=MS.gene53592.t1
chr1.2 exon 29119518 29119757 29119518 ID=MS.gene53592.t1.exon5;Parent=MS.gene53592.t1
chr1.2 CDS 29119518 29119757 29119518 ID=cds.MS.gene53592.t1;Parent=MS.gene53592.t1
Gene Sequence

>MS.gene53592

ATGGCCAAGGATTACCCAGATTTCTTCAAAGTTTTTTCTACAGAAAAGCACACTGAAAGAATGGTACGTACTACTTGCTTATTCTCAACCATGCTTTCTCCAATGTTTTTTATTCCTTTTTGATAAAAACCAGTTTGGTATGCAAAGAAATAATATGAAATGTATTGAAATAACTTGTGATGTAGTAGTTATTTACTACTAATTAATCTTCATCATTTTCTTTAATCTATTGCAGCGTATCCCTAATGCTTTTGTGAACTTGATGCGTTCAAAAAGGAAGGTCATAAAGGATTTCATCCTAAGAGATCGTAGAGGAAGAGATTGGCATGTCAAAGCTCGTCGTATTGGCGATGAGCTTTATTTTGACGATGGTTGGAAGCAATTTAGAGACGAAAATTCTTTAGAGGAGGATGATTTCCTTGTTTTTACACATATTGAAAATAACATATTCAGGTTCAAAATTCTTGAACTATCATCAATGTGTGAAAAGAAGAAGGTAAGTGACATGGAGGAAAATAACAATATGGAGGATGAGGAAGAGGACGGTGATGGTGATGATGATGATGACGATGACGAGGATGATGATTATGATTTTGATGCTGCTGCTGCTGCTGATGATGATATTATGGCAGAGGAAGAAGATCATGATGATGATATTATGGCAGAGGAAGAACATCATGATACCGATGATGATGATGGTGATGACGACGACGAGAATGAAAGAATGTACAAGGAAATTAGTAGAAGTAAACATCAACATTGTAGAACCTGCAAGTGCAAAGCTGGTAAGTTTTGACATCATACCGAAGATAAAACTACTATTTTGTTTTTTCATTCTATATGAGTTTGTGCATGCATGAGTCAGAATTTACCTGATGATTTATTTTGATTTAGCGGACATTGGGAGCAGCTCTGCACATTCAAAACCTGAAGACGACGAAATTGATGCTGAAATGTATATTCAACCCGGAAATCCACACTTCATTGCAAAATATATTCATTCCAGACCTAATGAATTGGTACAATTCTTTATGTCCATTACTTTTTTTTTTTTGACATGGAAGCATAGTTAAACGAGTGATTAGTCCATTTGCTTAACCCTAGGTAGAGGAGTTAGTCGAAATTTTCACCTTCGACTTGTCATTTCTTATCAATTTTCCTTTGTACTCTATGCCTGTCTAGTTATTCTCTTTATTACTTTTATGTCCAAGTTGTTGCAATTTTACTTGTCATAACTTGCAATCATTTCATGTTACTTCTTTGATGTACTTATTATGCCATCACTCTTCACATTTGAAGTTTATTCCAAAGAATGTTATCAAGGATTTCTGCCTCTGCTTCACAAAACATGTCACCCTTGAATGTTGCCACTGCAAGGTATAGTATAATTCCAATCTTTACCTTTCATTTTTCACTATTATGAAGTGGTATCCACTTTTGTTTAATGTGTTGATAATTTGATATTCAAGTTGCATTCATCCTTTTTTGGTTATGACAGGACATTCAGAGCAATGACTTAGCAGCCTATCATCACATCTTGCCACAAATAAGCAGAACACATATCGAAAAGAGAGGCCAAGTACGTACGTGGAGAGATGGTCGAGTACGTGTGCTAGGGTGGGAAGATTTTTGTAGAAAGTGCAATATCACAGAAAATGATAGTTGCTTATGCGAGATTGTGCTGCGTGAAGATGGAAAAATCGAAATGTTAAGGGTGCACATTGTTAGGAAGGAATGA
Protein sequence

>MS.gene53592.t1

MAKDYPDFFKVFSTEKHTERMRIPNAFVNLMRSKRKVIKDFILRDRRGRDWHVKARRIGDELYFDDGWKQFRDENSLEEDDFLVFTHIENNIFRFKILELSSMCEKKKVSDMEENNNMEDEEEDGDGDDDDDDDEDDDYDFDAAAAADDDIMAEEEDHDDDIMAEEEHHDTDDDDGDDDDENERMYKEISRSKHQHCRTCKCKAADIGSSSAHSKPEDDEIDAEMYIQPGNPHFIAKYIHSRPNELFIPKNVIKDFCLCFTKHVTLECCHCKDIQSNDLAAYHHILPQISRTHIEKRGQVRTWRDGRVRVLGWEDFCRKCNITENDSCLCEIVLREDGKIEMLRVHIVRKE