AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53621

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53621.t1 MTR_1g046680 98.770 244 3 0 1 244 1 244 0.0 498
MS.gene53621.t1 MTR_5g007600 71.489 235 52 3 1 235 1 220 7.94e-105 306
MS.gene53621.t1 MTR_5g007600 76.147 109 26 0 136 244 223 331 1.09e-56 184
MS.gene53621.t1 MTR_8g013950 47.340 188 84 7 63 241 114 295 3.84e-39 139
MS.gene53621.t1 MTR_8g076340 72.000 75 21 0 170 244 216 290 5.15e-36 129
MS.gene53621.t1 MTR_3g109760 44.554 202 69 8 63 241 95 276 2.14e-35 128
MS.gene53621.t1 MTR_2g094600 46.584 161 54 4 99 236 129 280 2.14e-35 128
MS.gene53621.t1 MTR_1g006650 43.810 210 71 7 63 241 110 303 5.96e-35 127
MS.gene53621.t1 MTR_3g117380 36.260 262 122 9 6 241 56 298 1.44e-34 126
MS.gene53621.t1 MTR_4g071590 50.000 140 44 5 124 239 141 278 2.77e-33 122
MS.gene53621.t1 MTR_4g471590 50.000 140 44 5 124 239 141 278 2.77e-33 122
MS.gene53621.t1 MTR_5g021340 71.233 73 21 0 172 244 300 372 3.24e-33 124
MS.gene53621.t1 MTR_6g032830 76.471 68 16 0 171 238 191 258 6.94e-33 120
MS.gene53621.t1 MTR_1g094930 70.130 77 21 1 160 236 137 211 7.39e-33 119
MS.gene53621.t1 MTR_7g085410 74.648 71 18 0 166 236 182 252 8.44e-33 120
MS.gene53621.t1 MTR_1g094930 70.130 77 21 1 160 236 232 306 2.79e-32 120
MS.gene53621.t1 MTR_4g058910 74.627 67 17 0 172 238 314 380 1.25e-31 120
MS.gene53621.t1 MTR_1g116010 71.642 67 19 0 170 236 162 228 1.76e-31 116
MS.gene53621.t1 MTR_7g446110 55.224 67 28 1 172 238 187 251 4.92e-19 83.6
MS.gene53621.t1 MTR_7g445980 54.286 70 29 2 170 238 31 98 6.04e-18 76.6
MS.gene53621.t1 MTR_7g445950 47.126 87 44 2 149 233 95 181 1.22e-17 78.2
MS.gene53621.t1 MTR_7g446070 46.739 92 45 3 149 238 107 196 2.45e-17 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53621.t1 AT3G24050 80.000 75 15 0 170 244 192 266 2.39e-40 140
MS.gene53621.t1 AT4G32890 39.636 275 98 11 7 241 20 266 6.27e-38 135
MS.gene53621.t1 AT4G36240 38.340 253 104 6 6 239 12 231 6.77e-36 127
MS.gene53621.t1 AT4G34680 46.479 142 63 3 111 242 112 250 4.77e-35 126
MS.gene53621.t1 AT4G34680 46.479 142 63 3 111 242 112 250 4.77e-35 126
MS.gene53621.t1 AT5G66320 79.412 68 14 0 172 239 249 316 8.54e-35 127
MS.gene53621.t1 AT5G66320 79.412 68 14 0 172 239 249 316 8.54e-35 127
MS.gene53621.t1 AT5G25830 77.143 70 16 0 172 241 219 288 1.19e-33 124
MS.gene53621.t1 AT3G51080 72.603 73 20 0 172 244 221 293 2.66e-33 122
MS.gene53621.t1 AT3G60530 75.362 69 17 0 171 239 157 225 2.79e-33 120
MS.gene53621.t1 AT1G08010 72.464 69 19 0 170 238 218 286 2.74e-32 120
MS.gene53621.t1 AT1G08010 72.464 69 19 0 170 238 218 286 2.74e-32 120
MS.gene53621.t1 AT1G08010 72.464 69 19 0 170 238 218 286 2.74e-32 120
MS.gene53621.t1 AT2G45050 75.000 68 17 0 172 239 179 246 3.73e-32 118
MS.gene53621.t1 AT1G08000 64.634 82 29 0 157 238 203 284 6.80e-32 119
MS.gene53621.t1 AT1G08000 64.634 82 29 0 157 238 203 284 6.80e-32 119
MS.gene53621.t1 AT3G54810 75.000 68 17 0 172 239 229 296 6.38e-31 117
MS.gene53621.t1 AT3G54810 75.000 68 17 0 172 239 229 296 6.38e-31 117
MS.gene53621.t1 AT3G45170 60.294 68 27 0 172 239 115 182 2.32e-27 104
MS.gene53621.t1 AT2G28340 66.667 63 21 0 174 236 193 255 9.56e-26 102

Find 86 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTTTCTCTTTGGAAGTTT+TGG 0.158917 1.2:+29442253 None:intergenic
CTTGTGCTGTTCTCGAGCTT+TGG 0.240759 1.2:+29442434 None:intergenic
TTGTGCTGTTCTCGAGCTTT+GGG 0.269770 1.2:+29442435 None:intergenic
GGTTTGTTTCACTTTCTCTT+TGG 0.274207 1.2:+29442244 None:intergenic
AGTGAAGGAAAGGCTTTCTT+AGG 0.280723 1.2:+29443134 None:intergenic
GTCCAACAAAGATGCATTTC+CGG 0.285678 1.2:-29442522 MS.gene53621:CDS
GACATTTCCTCCCAATGGTT+GGG 0.293078 1.2:+29442222 None:intergenic
CTATTTGCAGGACGGTATTC+AGG 0.302785 1.2:+29442095 None:intergenic
CGAAAAGTTGGGCTATTTGC+AGG 0.311001 1.2:+29442083 None:intergenic
CATGCATTACAAAGTGTCTT+TGG 0.315428 1.2:+29442149 None:intergenic
CAAAGTGTCTTTGGACCATT+TGG 0.337970 1.2:+29442158 None:intergenic
GAAGGTGGATCACTGCATTC+TGG 0.356189 1.2:+29443107 None:intergenic
GTTGTTTCCACGGGAATTGT+AGG 0.366200 1.2:+29442282 None:intergenic
TGGTTCTGTGGACGACCTCT+TGG 0.388206 1.2:-29443198 MS.gene53621:CDS
GATGAGGAAGCAGAAGGGAA+TGG 0.399465 1.2:-29442021 MS.gene53621:CDS
TTTGTAATGCATGTGGGGTT+AGG 0.415099 1.2:-29442139 MS.gene53621:CDS
ACTTACAGAAAGTGAATTGA+TGG 0.418526 1.2:+29443058 None:intergenic
CCACGGGAATTGTAGGTTTG+AGG 0.425374 1.2:+29442289 None:intergenic
AAGCAGAAGGGAATGGGAAC+GGG 0.428545 1.2:-29442014 MS.gene53621:CDS
TGTGATTGTAGCCACTTAAG+AGG 0.429240 1.2:+29442375 None:intergenic
AGCCGGAAATGCATCTTTGT+TGG 0.438765 1.2:+29442520 None:intergenic
TGCATGTCACTGCGAAAAGT+TGG 0.440834 1.2:+29442071 None:intergenic
ACATTTCCTCCCAATGGTTG+GGG 0.449081 1.2:+29442223 None:intergenic
ATTTGATGCTTCAAAAGATC+CGG 0.450692 1.2:+29442464 None:intergenic
TGAGTTGTCCTGTATTCAAC+CGG 0.452850 1.2:-29442483 MS.gene53621:CDS
CAAAAGATCCGGTTGAATAC+AGG 0.453486 1.2:+29442475 None:intergenic
GACTTGCTCCGTGCACGGAC+AGG 0.458205 1.2:+29442344 None:intergenic
TTCGCTCAATCCATTGGCCC+TGG 0.469600 1.2:-29443087 MS.gene53621:CDS
CCAGGAGGAGGTTGCAGAAG+AGG 0.477241 1.2:-29442558 MS.gene53621:intron
GTGGAGATGAGGAAGCAGAA+GGG 0.478182 1.2:-29442026 MS.gene53621:CDS
TTGCAGAAGAGGAGCTTGAA+TGG 0.482772 1.2:-29442547 MS.gene53621:CDS
ATGAGGAAGCAGAAGGGAAT+GGG 0.486460 1.2:-29442020 MS.gene53621:CDS
GTGAAACAAACCCCAACCAT+TGG 0.493226 1.2:-29442233 MS.gene53621:CDS
TTGGTACTCGGTTGTTTCCA+CGG 0.494137 1.2:+29442272 None:intergenic
AACAGCATTACCCTCTTAAG+TGG 0.500575 1.2:-29442386 MS.gene53621:CDS
GCATGTCACTGCGAAAAGTT+GGG 0.502991 1.2:+29442072 None:intergenic
TGGTACTCGGTTGTTTCCAC+GGG 0.505676 1.2:+29442273 None:intergenic
CAATGGCGTGCAGGTCCAAA+TGG 0.508669 1.2:-29442173 MS.gene53621:CDS
TTGGATTTCTCATCTGACAT+AGG 0.508816 1.2:-29443179 MS.gene53621:CDS
AGTGGAGATGAGGAAGCAGA+AGG 0.514467 1.2:-29442027 MS.gene53621:CDS
TTCTGGTTTGAGTGAAGGAA+AGG 0.515866 1.2:+29443124 None:intergenic
TGACATTTCCTCCCAATGGT+TGG 0.516568 1.2:+29442221 None:intergenic
TGTGGGGTTAGGTATAAGTC+TGG 0.520333 1.2:-29442128 MS.gene53621:CDS
TCCACCTTCGCTCAATCCAT+TGG 0.521499 1.2:-29443093 MS.gene53621:CDS
GATGGAGGCCCTTGGTTCTG+TGG 0.522422 1.2:-29443210 MS.gene53621:CDS
TGAAACAAACCCCAACCATT+GGG 0.524385 1.2:-29442232 MS.gene53621:CDS
GTGAATTGATGGGATCATCC+AGG 0.528433 1.2:+29443069 None:intergenic
ATGGGATCATCCAGGGCCAA+TGG 0.530010 1.2:+29443077 None:intergenic
CTGCATTCTGGTTTGAGTGA+AGG 0.532294 1.2:+29443119 None:intergenic
TCATCTCCACTACTTTCCTG+TGG 0.533667 1.2:+29442039 None:intergenic
TCTCGTAGCAAGCCTCGCCT+TGG 0.534627 1.2:-29442323 MS.gene53621:CDS
GATACTCCACAATGGCGTGC+AGG 0.539263 1.2:-29442182 MS.gene53621:CDS
TCAACAAACGTTTCAACAGC+CGG 0.539345 1.2:+29442503 None:intergenic
TTGAGGCATCAGCAAGGCCA+AGG 0.541565 1.2:+29442306 None:intergenic
GAAGCAGAAGGGAATGGGAA+CGG 0.544276 1.2:-29442015 MS.gene53621:CDS
GCCAATGGATTGAGCGAAGG+TGG 0.546210 1.2:+29443092 None:intergenic
ACTTAAGAGGGTAATGCTGT+TGG 0.550014 1.2:+29442388 None:intergenic
CATCTCCACTACTTTCCTGT+GGG 0.557649 1.2:+29442040 None:intergenic
TGAAGTTCCCTGTCCGTGCA+CGG 0.571256 1.2:-29442352 MS.gene53621:CDS
TTTGGACCTGCACGCCATTG+TGG 0.572595 1.2:+29442176 None:intergenic
ACTTGCTCCGTGCACGGACA+GGG 0.574860 1.2:+29442345 None:intergenic
TACGAGACTTGCTCCGTGCA+CGG 0.578328 1.2:+29442339 None:intergenic
ATGATGACATTTCCTCCCAA+TGG 0.580868 1.2:+29442217 None:intergenic
ACAGGAAAGTAGTGGAGATG+AGG 0.582547 1.2:-29442037 MS.gene53621:CDS
TGTGCTGTTCTCGAGCTTTG+GGG 0.589584 1.2:+29442436 None:intergenic
TGAATTGATGGGATCATCCA+GGG 0.600445 1.2:+29443070 None:intergenic
GCATCAGCAAGGCCAAGGCG+AGG 0.601336 1.2:+29442311 None:intergenic
GACACTTTGTAATGCATGTG+GGG 0.601729 1.2:-29442144 MS.gene53621:CDS
AAGACACTTTGTAATGCATG+TGG 0.602236 1.2:-29442146 MS.gene53621:CDS
TAATTCCCACAGGAAAGTAG+TGG 0.604924 1.2:-29442045 MS.gene53621:CDS
GAGGTCGTCCACAGAACCAA+GGG 0.608143 1.2:+29443202 None:intergenic
AGACACTTTGTAATGCATGT+GGG 0.608576 1.2:-29442145 MS.gene53621:CDS
CTTACAGAAAGTGAATTGAT+GGG 0.611737 1.2:+29443059 None:intergenic
GTGATTGTAGCCACTTAAGA+GGG 0.622729 1.2:+29442376 None:intergenic
ACATGCATTCTAATTCCCAC+AGG 0.630282 1.2:-29442055 MS.gene53621:CDS
AACAAACCCCAACCATTGGG+AGG 0.635541 1.2:-29442229 MS.gene53621:CDS
AGGGCCAATGGATTGAGCGA+AGG 0.639769 1.2:+29443089 None:intergenic
AGAGGTCGTCCACAGAACCA+AGG 0.642044 1.2:+29443201 None:intergenic
GGGAGGAAATGTCATCATTG+TGG 0.646984 1.2:-29442212 MS.gene53621:CDS
CCTCAAACCTACAATTCCCG+TGG 0.649034 1.2:-29442289 MS.gene53621:CDS
GACGGTATTCAGGTACAAGT+CGG 0.649278 1.2:+29442105 None:intergenic
AAGTTGGGCTATTTGCAGGA+CGG 0.658089 1.2:+29442087 None:intergenic
TTTCTCATCTGACATAGGTG+AGG 0.665571 1.2:-29443174 MS.gene53621:CDS
GTAGGTTTGAGGCATCAGCA+AGG 0.716777 1.2:+29442300 None:intergenic
TGTCAGATGAGAAATCCAAG+AGG 0.747403 1.2:+29443183 None:intergenic
GAGTTGATGATACTCCACAA+TGG 0.801331 1.2:-29442190 MS.gene53621:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTATTTTGAAAAGGTTAA+TGG + chr1.2:29442195-29442214 None:intergenic 20.0%
! AACTCATAAGTATGGAAAAA+AGG + chr1.2:29442607-29442626 None:intergenic 25.0%
! AAGGAAAACAAGTAAAAATC+TGG + chr1.2:29442360-29442379 None:intergenic 25.0%
!!! ATCAAGCAAGTATTTTGAAA+AGG + chr1.2:29442202-29442221 None:intergenic 25.0%
ACTTACAGAAAGTGAATTGA+TGG + chr1.2:29442164-29442183 None:intergenic 30.0%
ATTTGATGCTTCAAAAGATC+CGG + chr1.2:29442758-29442777 None:intergenic 30.0%
CACTAGCTAATATTCTACTT+TGG + chr1.2:29442334-29442353 None:intergenic 30.0%
CATGAAAACTTTGATGCAAA+CGG + chr1.2:29442451-29442470 None:intergenic 30.0%
CTAATATTCTACTTTGGACA+AGG + chr1.2:29442328-29442347 None:intergenic 30.0%
CTTACAGAAAGTGAATTGAT+GGG + chr1.2:29442163-29442182 None:intergenic 30.0%
CTTCACATAACTCATAAGTA+TGG + chr1.2:29442615-29442634 None:intergenic 30.0%
TCCTTTAAGCATCAATGTAT+TGG - chr1.2:29442375-29442394 MS.gene53621:CDS 30.0%
TTCACTTTCTGTAAGTTTGA+CGG - chr1.2:29442168-29442187 MS.gene53621:CDS 30.0%
!!! TATCTAGTTTTGACTTGTTG+AGG - chr1.2:29442226-29442245 MS.gene53621:CDS 30.0%
AAGACACTTTGTAATGCATG+TGG - chr1.2:29443073-29443092 MS.gene53621:CDS 35.0%
AGACACTTTGTAATGCATGT+GGG - chr1.2:29443074-29443093 MS.gene53621:CDS 35.0%
GCCAATACATTGATGCTTAA+AGG + chr1.2:29442379-29442398 None:intergenic 35.0%
TCCTGGAACATGAAAATAAG+CGG + chr1.2:29442647-29442666 None:intergenic 35.0%
TTGGATTTCTCATCTGACAT+AGG - chr1.2:29442040-29442059 MS.gene53621:CDS 35.0%
! AGCATCAATGTATTGGCATT+TGG - chr1.2:29442382-29442401 MS.gene53621:CDS 35.0%
! CATGCATTACAAAGTGTCTT+TGG + chr1.2:29443073-29443092 None:intergenic 35.0%
! GGTTTGTTTCACTTTCTCTT+TGG + chr1.2:29442978-29442997 None:intergenic 35.0%
!! CACTTTCTCTTTGGAAGTTT+TGG + chr1.2:29442969-29442988 None:intergenic 35.0%
!! TCTTTGGAAGTTTTGGTACT+CGG + chr1.2:29442962-29442981 None:intergenic 35.0%
AACAGCATTACCCTCTTAAG+TGG - chr1.2:29442833-29442852 MS.gene53621:intron 40.0%
ACATGCATTCTAATTCCCAC+AGG - chr1.2:29443164-29443183 MS.gene53621:CDS 40.0%
ACTTAAGAGGGTAATGCTGT+TGG + chr1.2:29442834-29442853 None:intergenic 40.0%
AGTGAAGGAAAGGCTTTCTT+AGG + chr1.2:29442088-29442107 None:intergenic 40.0%
ATGATGACATTTCCTCCCAA+TGG + chr1.2:29443005-29443024 None:intergenic 40.0%
CAAAAGATCCGGTTGAATAC+AGG + chr1.2:29442747-29442766 None:intergenic 40.0%
CACTTGTGATCACTGTTTCA+TGG - chr1.2:29442471-29442490 MS.gene53621:CDS 40.0%
GACACTTTGTAATGCATGTG+GGG - chr1.2:29443075-29443094 MS.gene53621:CDS 40.0%
GTCCAACAAAGATGCATTTC+CGG - chr1.2:29442697-29442716 MS.gene53621:intron 40.0%
GTGATTGTAGCCACTTAAGA+GGG + chr1.2:29442846-29442865 None:intergenic 40.0%
TAATTCCCACAGGAAAGTAG+TGG - chr1.2:29443174-29443193 MS.gene53621:CDS 40.0%
TCAACAAACGTTTCAACAGC+CGG + chr1.2:29442719-29442738 None:intergenic 40.0%
TGAAACAAACCCCAACCATT+GGG - chr1.2:29442987-29443006 MS.gene53621:intron 40.0%
TGAATTGATGGGATCATCCA+GGG + chr1.2:29442152-29442171 None:intergenic 40.0%
TGAGTTGTCCTGTATTCAAC+CGG - chr1.2:29442736-29442755 MS.gene53621:intron 40.0%
TGTCAGATGAGAAATCCAAG+AGG + chr1.2:29442039-29442058 None:intergenic 40.0%
TGTGATTGTAGCCACTTAAG+AGG + chr1.2:29442847-29442866 None:intergenic 40.0%
TTTCTCATCTGACATAGGTG+AGG - chr1.2:29442045-29442064 MS.gene53621:CDS 40.0%
TTTGTAATGCATGTGGGGTT+AGG - chr1.2:29443080-29443099 MS.gene53621:CDS 40.0%
! CAAAGTGTCTTTGGACCATT+TGG + chr1.2:29443064-29443083 None:intergenic 40.0%
! TATTTTCATGTTCCAGGAGG+AGG - chr1.2:29442649-29442668 MS.gene53621:intron 40.0%
!! GAGTTGATGATACTCCACAA+TGG - chr1.2:29443029-29443048 MS.gene53621:intron 40.0%
!! GCTTATTTTCATGTTCCAGG+AGG - chr1.2:29442646-29442665 MS.gene53621:intron 40.0%
!! TTCTGGTTTGAGTGAAGGAA+AGG + chr1.2:29442098-29442117 None:intergenic 40.0%
AAGTTGGGCTATTTGCAGGA+CGG + chr1.2:29443135-29443154 None:intergenic 45.0%
ACATTTCCTCCCAATGGTTG+GGG + chr1.2:29442999-29443018 None:intergenic 45.0%
ACCGCAGGCATTAACAATCT+CGG + chr1.2:29442412-29442431 None:intergenic 45.0%
AGCCGGAAATGCATCTTTGT+TGG + chr1.2:29442702-29442721 None:intergenic 45.0%
ATGAGGAAGCAGAAGGGAAT+GGG - chr1.2:29443199-29443218 MS.gene53621:CDS 45.0%
CATCTCCACTACTTTCCTGT+GGG + chr1.2:29443182-29443201 None:intergenic 45.0%
CGAAAAGTTGGGCTATTTGC+AGG + chr1.2:29443139-29443158 None:intergenic 45.0%
CTATTTGCAGGACGGTATTC+AGG + chr1.2:29443127-29443146 None:intergenic 45.0%
GACATTTCCTCCCAATGGTT+GGG + chr1.2:29443000-29443019 None:intergenic 45.0%
GACGGTATTCAGGTACAAGT+CGG + chr1.2:29443117-29443136 None:intergenic 45.0%
GCATGTCACTGCGAAAAGTT+GGG + chr1.2:29443150-29443169 None:intergenic 45.0%
GGAATTCCTCGAGTAAACTC+AGG + chr1.2:29442586-29442605 None:intergenic 45.0%
GGGAGGAAATGTCATCATTG+TGG - chr1.2:29443007-29443026 MS.gene53621:intron 45.0%
GTGAAACAAACCCCAACCAT+TGG - chr1.2:29442986-29443005 MS.gene53621:intron 45.0%
GTGAATTGATGGGATCATCC+AGG + chr1.2:29442153-29442172 None:intergenic 45.0%
GTTTCATGGTTTCCATGTCG+TGG - chr1.2:29442485-29442504 MS.gene53621:CDS 45.0%
TCATCTCCACTACTTTCCTG+TGG + chr1.2:29443183-29443202 None:intergenic 45.0%
TCCGAGATTGTTAATGCCTG+CGG - chr1.2:29442408-29442427 MS.gene53621:CDS 45.0%
TGAATGCCTGAGTTTACTCG+AGG - chr1.2:29442577-29442596 MS.gene53621:intron 45.0%
TGACATTTCCTCCCAATGGT+TGG + chr1.2:29443001-29443020 None:intergenic 45.0%
TGCATGTCACTGCGAAAAGT+TGG + chr1.2:29443151-29443170 None:intergenic 45.0%
TGTGGGGTTAGGTATAAGTC+TGG - chr1.2:29443091-29443110 MS.gene53621:CDS 45.0%
TTGCAGAAGAGGAGCTTGAA+TGG - chr1.2:29442672-29442691 MS.gene53621:intron 45.0%
! ACAGGAAAGTAGTGGAGATG+AGG - chr1.2:29443182-29443201 MS.gene53621:CDS 45.0%
! GTTGTTTCCACGGGAATTGT+AGG + chr1.2:29442940-29442959 None:intergenic 45.0%
! TTGGTACTCGGTTGTTTCCA+CGG + chr1.2:29442950-29442969 None:intergenic 45.0%
! TTGTGCTGTTCTCGAGCTTT+GGG + chr1.2:29442787-29442806 None:intergenic 45.0%
!! CTGCATTCTGGTTTGAGTGA+AGG + chr1.2:29442103-29442122 None:intergenic 45.0%
!! GCCGCTTATTTTCATGTTCC+AGG - chr1.2:29442643-29442662 MS.gene53621:intron 45.0%
AACAAACCCCAACCATTGGG+AGG - chr1.2:29442990-29443009 MS.gene53621:intron 50.0%
AAGCAGAAGGGAATGGGAAC+GGG - chr1.2:29443205-29443224 MS.gene53621:CDS 50.0%
AGTGGAGATGAGGAAGCAGA+AGG - chr1.2:29443192-29443211 MS.gene53621:CDS 50.0%
CCACGGGAATTGTAGGTTTG+AGG + chr1.2:29442933-29442952 None:intergenic 50.0%
CCTCAAACCTACAATTCCCG+TGG - chr1.2:29442930-29442949 MS.gene53621:intron 50.0%
GAAGCAGAAGGGAATGGGAA+CGG - chr1.2:29443204-29443223 MS.gene53621:CDS 50.0%
GAAGGTGGATCACTGCATTC+TGG + chr1.2:29442115-29442134 None:intergenic 50.0%
GATGAGGAAGCAGAAGGGAA+TGG - chr1.2:29443198-29443217 MS.gene53621:CDS 50.0%
GTGGAGATGAGGAAGCAGAA+GGG - chr1.2:29443193-29443212 MS.gene53621:CDS 50.0%
TCCACCTTCGCTCAATCCAT+TGG - chr1.2:29442126-29442145 MS.gene53621:CDS 50.0%
! CTTGTGCTGTTCTCGAGCTT+TGG + chr1.2:29442788-29442807 None:intergenic 50.0%
! GAATTTGTCGTTTGCACCGC+AGG + chr1.2:29442427-29442446 None:intergenic 50.0%
! GTAGGTTTGAGGCATCAGCA+AGG + chr1.2:29442922-29442941 None:intergenic 50.0%
! TGGTACTCGGTTGTTTCCAC+GGG + chr1.2:29442949-29442968 None:intergenic 50.0%
! TGTGCTGTTCTCGAGCTTTG+GGG + chr1.2:29442786-29442805 None:intergenic 50.0%
AGAGATGCAGCTCCACGACA+TGG + chr1.2:29442500-29442519 None:intergenic 55.0%
AGAGGTCGTCCACAGAACCA+AGG + chr1.2:29442021-29442040 None:intergenic 55.0%
ATGGGATCATCCAGGGCCAA+TGG + chr1.2:29442145-29442164 None:intergenic 55.0%
CAATGGCGTGCAGGTCCAAA+TGG - chr1.2:29443046-29443065 MS.gene53621:intron 55.0%
GAGGTCGTCCACAGAACCAA+GGG + chr1.2:29442020-29442039 None:intergenic 55.0%
GATACTCCACAATGGCGTGC+AGG - chr1.2:29443037-29443056 MS.gene53621:intron 55.0%
TGAAGTTCCCTGTCCGTGCA+CGG - chr1.2:29442867-29442886 MS.gene53621:intron 55.0%
TGTCGTGGAGCTGCATCTCT+CGG - chr1.2:29442500-29442519 MS.gene53621:CDS 55.0%
TTCGCTCAATCCATTGGCCC+TGG - chr1.2:29442132-29442151 MS.gene53621:CDS 55.0%
TTTGGACCTGCACGCCATTG+TGG + chr1.2:29443046-29443065 None:intergenic 55.0%
! TACGAGACTTGCTCCGTGCA+CGG + chr1.2:29442883-29442902 None:intergenic 55.0%
! TGGTTCTGTGGACGACCTCT+TGG - chr1.2:29442021-29442040 MS.gene53621:CDS 55.0%
! TTGAGGCATCAGCAAGGCCA+AGG + chr1.2:29442916-29442935 None:intergenic 55.0%
!! AGGGCCAATGGATTGAGCGA+AGG + chr1.2:29442133-29442152 None:intergenic 55.0%
!! GCCAATGGATTGAGCGAAGG+TGG + chr1.2:29442130-29442149 None:intergenic 55.0%
ACTTGCTCCGTGCACGGACA+GGG + chr1.2:29442877-29442896 None:intergenic 60.0%
CCAGGAGGAGGTTGCAGAAG+AGG - chr1.2:29442661-29442680 MS.gene53621:intron 60.0%
CCTCTTCTGCAACCTCCTCC+TGG + chr1.2:29442664-29442683 None:intergenic 60.0%
TCTCGTAGCAAGCCTCGCCT+TGG - chr1.2:29442896-29442915 MS.gene53621:intron 60.0%
GCATCAGCAAGGCCAAGGCG+AGG + chr1.2:29442911-29442930 None:intergenic 65.0%
! GACTTGCTCCGTGCACGGAC+AGG + chr1.2:29442878-29442897 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 29442010 29443231 29442010 ID=MS.gene53621
chr1.2 mRNA 29442010 29443231 29442010 ID=MS.gene53621.t1;Parent=MS.gene53621
chr1.2 exon 29443064 29443231 29443064 ID=MS.gene53621.t1.exon1;Parent=MS.gene53621.t1
chr1.2 CDS 29443064 29443231 29443064 ID=cds.MS.gene53621.t1;Parent=MS.gene53621.t1
chr1.2 exon 29442010 29442576 29442010 ID=MS.gene53621.t1.exon2;Parent=MS.gene53621.t1
chr1.2 CDS 29442010 29442576 29442010 ID=cds.MS.gene53621.t1;Parent=MS.gene53621.t1
Gene Sequence

>MS.gene53621

ATGGAGGCCCTTGGTTCTGTGGACGACCTCTTGGATTTCTCATCTGACATAGGTGAGGATGATGATGATGACAAACCTAAGAAAGCCTTTCCTTCACTCAAACCAGAATGCAGTGATCCACCTTCGCTCAATCCATTGGCCCTGGATGATCCCATCAATTCACTTTCTGTAAGTTTGACGGTCCATTAACCTTTTCAAAATACTTGCTTGATTTTTTATCTAGTTTTGACTTGTTGAGGTGTGATCATGAAAGCGAAAGCATGTGTGAATTATGATTTGTTTTTAATCAATTAGAATTTTGATATATTATATTGTCCTTGTCCAAAGTAGAATATTAGCTAGTGTTTCCAGATTTTTACTTGTTTTCCTTTAAGCATCAATGTATTGGCATTTGGAATTCCGAGATTGTTAATGCCTGCGGTGCAAACGACAAATTCTCCGTTTGCATCAAAGTTTTCATGCACTTGTGATCACTGTTTCATGGTTTCCATGTCGTGGAGCTGCATCTCTCGGCGTGAATACTAATTTCTTGATCGAATTGTTGTGTCGTTGACTTGTTGCATCAGTTGAATGCCTGAGTTTACTCGAGGAATTCCTTTTTTCCATACTTATGAGTTATGTGAAGTTTAATGTGCCGCTTATTTTCATGTTCCAGGAGGAGGTTGCAGAAGAGGAGCTTGAATGGCTGTCCAACAAAGATGCATTTCCGGCTGTTGAAACGTTTGTTGAGTTGTCCTGTATTCAACCGGATCTTTTGAAGCATCAAATGACATCCCCAAAGCTCGAGAACAGCACAAGCAGCAGCAACAGCAATAACAGTTCCAACAGCATTACCCTCTTAAGTGGCTACAATCACATGAAGTTCCCTGTCCGTGCACGGAGCAAGTCTCGTAGCAAGCCTCGCCTTGGCCTTGCTGATGCCTCAAACCTACAATTCCCGTGGAAACAACCGAGTACCAAAACTTCCAAAGAGAAAGTGAAACAAACCCCAACCATTGGGAGGAAATGTCATCATTGTGGAGTTGATGATACTCCACAATGGCGTGCAGGTCCAAATGGTCCAAAGACACTTTGTAATGCATGTGGGGTTAGGTATAAGTCTGGCCGACTTGTACCTGAATACCGTCCTGCAAATAGCCCAACTTTTCGCAGTGACATGCATTCTAATTCCCACAGGAAAGTAGTGGAGATGAGGAAGCAGAAGGGAATGGGAACGGGTTGA
Protein sequence

>MS.gene53621.t1

MEALGSVDDLLDFSSDIGEDDDDDKPKKAFPSLKPECSDPPSLNPLALDDPINSLSEEVAEEELEWLSNKDAFPAVETFVELSCIQPDLLKHQMTSPKLENSTSSSNSNNSSNSITLLSGYNHMKFPVRARSKSRSKPRLGLADASNLQFPWKQPSTKTSKEKVKQTPTIGRKCHHCGVDDTPQWRAGPNGPKTLCNACGVRYKSGRLVPEYRPANSPTFRSDMHSNSHRKVVEMRKQKGMGTG