AlfalfaGEDB | MS.gene53749

M. sativa cultivar XinJiangDaYe / MS.gene53749

KEGG: -
GO: GO:0000976 GO:0000981 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0035326 GO:0043565 GO:0044212 GO:0045893 GO:0045935 GO:0045944 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53749.t1	XP_003605494.2	74.7	217	42	1	3	219	28	231	7.60E-82	313.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53749.t1	Q9FJK3	50.7	134	65	1	2	135	27	159	2.2e-29	130.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53749.t1	G7JN54	74.7	217	42	1	3	219	28	231	5.5e-82	313.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene53749.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53749.t1	MTR_4g032290	80.751	213	28	1	7	219	32	231	1.89e-124	352
MS.gene53749.t1	MTR_4g031910	79.812	213	28	1	7	219	32	229	5.95e-119	337
MS.gene53749.t1	MTR_4g032620	58.257	218	78	2	2	219	27	231	1.34e-85	253
MS.gene53749.t1	MTR_8g036130	59.471	227	77	4	2	219	27	247	7.32e-84	249
MS.gene53749.t1	MTR_4g032260	82.400	125	22	0	7	131	32	156	1.17e-74	223
MS.gene53749.t1	MTR_3g065100	44.811	212	110	3	7	216	32	238	3.94e-53	171
MS.gene53749.t1	MTR_4g032270	74.545	110	14	1	110	219	36	131	5.73e-52	164
MS.gene53749.t1	MTR_3g466980	55.039	129	57	1	2	130	27	154	3.32e-48	157
MS.gene53749.t1	MTR_2g035610	74.725	91	23	0	7	97	32	122	3.73e-42	139
MS.gene53749.t1	MTR_3g466830	41.606	137	79	1	2	138	26	161	1.77e-34	120
MS.gene53749.t1	MTR_4g063790	39.568	139	84	0	2	140	27	165	8.75e-33	117
MS.gene53749.t1	MTR_3g465410	39.416	137	83	0	4	140	1	137	9.60e-33	115
MS.gene53749.t1	MTR_3g466900	40.000	130	77	1	7	136	2	130	1.72e-31	112
MS.gene53749.t1	MTR_3g467080	39.231	130	79	0	2	131	27	156	3.60e-29	107
MS.gene53749.t1	MTR_3g466890	38.519	135	82	1	2	136	26	159	3.64e-29	107
MS.gene53749.t1	MTR_5g075380	39.062	128	77	1	2	129	27	153	1.07e-28	105
MS.gene53749.t1	MTR_3g466930	37.692	130	80	1	7	136	2	130	1.32e-28	104
MS.gene53749.t1	MTR_7g055800	57.303	89	23	1	6	94	18	91	2.57e-28	102
MS.gene53749.t1	MTR_3g031100	39.231	130	79	0	2	131	27	156	4.15e-28	106
MS.gene53749.t1	MTR_2g016210	36.154	130	83	0	2	131	27	156	6.01e-26	101
MS.gene53749.t1	MTR_5g047580	36.923	130	80	2	2	131	27	154	9.93e-24	93.2
MS.gene53749.t1	MTR_2g035590	57.732	97	40	1	49	144	1	97	1.03e-23	91.3
MS.gene53749.t1	MTR_4g028800	86.364	44	6	0	2	45	25	68	6.58e-23	88.2
MS.gene53749.t1	MTR_7g055940	56.579	76	18	1	6	81	18	78	3.83e-22	86.7
MS.gene53749.t1	MTR_1g084950	35.200	125	79	2	7	131	32	154	1.04e-21	87.8
MS.gene53749.t1	MTR_1g077320	34.400	125	80	2	7	131	32	154	3.49e-21	86.3
MS.gene53749.t1	MTR_1g077300	35.385	130	80	2	7	136	32	157	3.90e-21	86.3
MS.gene53749.t1	MTR_1g090697	33.333	132	86	2	2	133	27	156	8.06e-21	85.5
MS.gene53749.t1	MTR_1g077390	34.615	130	83	2	2	131	27	154	9.69e-21	85.1
MS.gene53749.t1	MTR_3g093900	34.746	118	77	0	2	119	30	147	1.46e-20	89.7
MS.gene53749.t1	MTR_7g011950	34.400	125	80	2	7	131	32	154	2.32e-20	84.3
MS.gene53749.t1	MTR_3g031240	31.818	132	90	0	2	133	27	158	3.68e-20	84.3
MS.gene53749.t1	MTR_4g028720	33.600	125	81	2	7	131	32	154	4.71e-20	83.6
MS.gene53749.t1	MTR_1g090710	30.303	132	91	1	2	133	27	157	1.88e-19	82.0
MS.gene53749.t1	MTR_4g019670	41.584	101	59	0	2	102	30	130	1.99e-19	86.3
MS.gene53749.t1	MTR_1g090783	31.538	130	87	2	2	131	26	153	2.37e-19	81.6
MS.gene53749.t1	MTR_7g106510	39.806	103	62	0	2	104	30	132	1.76e-18	83.6
MS.gene53749.t1	MTR_2g035580	34.615	130	84	1	2	131	27	155	8.69e-18	77.4
MS.gene53749.t1	MTR_7g055790	49.412	85	25	1	2	86	85	151	5.65e-15	69.7
MS.gene53749.t1	MTR_5g047560	39.474	76	45	1	17	92	26	100	1.90e-12	62.8
MS.gene53749.t1	MTR_4g084780	57.692	52	22	0	49	100	1	52	4.46e-12	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53749.t1	AT5G48670	49.612	129	64	1	7	135	32	159	3.21e-36	129
MS.gene53749.t1	AT1G65300	45.736	129	69	1	2	130	26	153	1.53e-33	122
MS.gene53749.t1	AT5G27810	56.989	93	40	0	4	96	1	93	4.79e-32	113
MS.gene53749.t1	AT1G65330	40.127	157	80	2	2	158	26	168	1.09e-31	117
MS.gene53749.t1	AT5G26630	35.326	184	106	2	9	192	34	204	1.43e-29	110
MS.gene53749.t1	AT5G26650	38.028	142	87	1	2	143	25	165	4.26e-29	112
MS.gene53749.t1	AT5G27960	37.324	142	88	1	2	143	25	165	2.23e-28	109
MS.gene53749.t1	AT1G31640	40.157	127	74	2	2	128	26	150	1.50e-26	106
MS.gene53749.t1	AT1G31630	34.395	157	88	3	2	158	26	167	1.77e-26	104
MS.gene53749.t1	AT3G05860	40.496	121	71	1	2	122	27	146	2.28e-22	90.9
MS.gene53749.t1	AT3G05860	40.496	121	71	1	2	122	27	146	7.62e-22	90.5
MS.gene53749.t1	AT3G05860	40.496	121	71	1	2	122	27	146	7.69e-22	90.5
MS.gene53749.t1	AT2G28700	36.508	126	75	2	2	125	27	149	1.48e-19	85.5
MS.gene53749.t1	AT5G26580	34.109	129	70	2	2	130	27	140	2.04e-17	79.7
MS.gene53749.t1	AT1G22590	28.462	130	89	1	2	131	27	152	1.41e-16	74.3
MS.gene53749.t1	AT5G06500	36.458	96	61	0	2	97	27	122	2.52e-13	67.4
MS.gene53749.t1	AT2G40210	34.862	109	71	0	1	109	26	134	1.25e-12	66.2
MS.gene53749.t1	AT2G15660	23.926	163	102	4	13	173	31	173	1.04e-11	62.8

Find 0 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATGTCATCATCATTAAT+AGG	-	33302:3246-3265	MS.gene53749:intergenic	20.0%
!!	GAAACAAAGAAAAGATAATA+GGG	+	33302:2939-2958	MS.gene53749:CDS	20.0%
!!	TGAAACAAAGAAAAGATAAT+AGG	+	33302:2938-2957	MS.gene53749:CDS	20.0%
!	TATGCAAAGTCAATTATTGA+TGG	+	33302:3263-3282	MS.gene53749:CDS	25.0%
	AGTGAAGAAATGGCAATTAT+GGG	+	33302:3201-3220	MS.gene53749:CDS	30.0%
!!	TCAATTATTGATGGACTCAA+TGG	+	33302:3272-3291	MS.gene53749:CDS	30.0%
	AAACCAAGTCCAACTTGAAA+TGG	+	33302:3140-3159	MS.gene53749:CDS	35.0%
	AAGAGACTTTCTTGACACAA+AGG	+	33302:2890-2909	MS.gene53749:CDS	35.0%
	AGAGACTTTCTTGACACAAA+GGG	+	33302:2891-2910	MS.gene53749:CDS	35.0%
	ATGGCTCAATGTCTTAATGA+AGG	+	33302:2982-3001	MS.gene53749:CDS	35.0%
	CATTAATAGGTATCCCAACA+TGG	-	33302:3233-3252	MS.gene53749:intergenic	35.0%
	GAGTGAAGAAATGGCAATTA+TGG	+	33302:3200-3219	MS.gene53749:CDS	35.0%
	GCACAACAATATGTCAACAA+TGG	+	33302:3014-3033	MS.gene53749:CDS	35.0%
	TACCAACGTGATTCAAATTG+TGG	-	33302:3068-3087	MS.gene53749:intergenic	35.0%
	TGGATATGAGTTATCTTGCT+TGG	+	33302:3034-3053	MS.gene53749:CDS	35.0%
	TTTGTGTCAAGAAAGTCTCT+TGG	-	33302:2891-2910	MS.gene53749:intergenic	35.0%
!!	ATTATTGATGGACTCAATGG+TGG	+	33302:3275-3294	MS.gene53749:CDS	35.0%
!!	TGAATTTTGAGAGCACAGAT+TGG	-	33302:2828-2847	MS.gene53749:intergenic	35.0%
	AGCATCTAGGAGTGAAGAAA+TGG	+	33302:3191-3210	MS.gene53749:CDS	40.0%
	GAAATGGCAATTATGGGTCA+TGG	+	33302:3207-3226	MS.gene53749:CDS	40.0%
	GACCACAATTTGAATCACGT+TGG	+	33302:3063-3082	MS.gene53749:CDS	40.0%
	GCAAGAGATGACAATACTCA+TGG	+	33302:2963-2982	MS.gene53749:CDS	40.0%
	TCAGAATTGACCTCACCAAA+TGG	-	33302:3324-3343	MS.gene53749:intergenic	40.0%
!!	GTGAGGTCAATTCTGAAGTT+TGG	+	33302:3328-3347	MS.gene53749:CDS	40.0%
	ACAAGCTTCAATGCCACAGA+GGG	-	33302:2746-2765	MS.gene53749:intergenic	45.0%
	ATGAGCCTCAACCTGAGATT+TGG	+	33302:2785-2804	MS.gene53749:CDS	45.0%
	ATTATGGGTCATGGCCATGT+TGG	+	33302:3216-3235	MS.gene53749:CDS	45.0%
	CCAAGTCCAACTTGAAATGG+CGG	+	33302:3143-3162	MS.gene53749:CDS	45.0%
	CTCATAAGGGCCATACACTA+TGG	-	33302:2770-2789	MS.gene53749:intergenic	45.0%
	GAAGCTTGTGCCATAGTGTA+TGG	+	33302:2757-2776	MS.gene53749:CDS	45.0%
	TTATGGGTCATGGCCATGTT+GGG	+	33302:3217-3236	MS.gene53749:CDS	45.0%
	TTGGTAGACGTTTGGAAGCA+TGG	+	33302:3082-3101	MS.gene53749:CDS	45.0%
!	GAATCACGTTGGTAGACGTT+TGG	+	33302:3074-3093	MS.gene53749:CDS	45.0%
!	TGGTAGACGTTTGGAAGCAT+GGG	+	33302:3083-3102	MS.gene53749:CDS	45.0%
!	TTGTTGCCGCCATTTCAAGT+TGG	-	33302:3152-3171	MS.gene53749:intergenic	45.0%
!!	ATCTCAGGTTGAGGCTCATA+AGG	-	33302:2784-2803	MS.gene53749:intergenic	45.0%
!!	TCTCAGGTTGAGGCTCATAA+GGG	-	33302:2783-2802	MS.gene53749:intergenic	45.0%
	CACAAGCTTCAATGCCACAG+AGG	-	33302:2747-2766	MS.gene53749:intergenic	50.0%
	GATGAACTTAGCACCCTCTG+TGG	+	33302:2730-2749	MS.gene53749:CDS	50.0%
	TCTGGTGAAGGCCAAATCTC+AGG	-	33302:2799-2818	MS.gene53749:intergenic	50.0%
	TCTTCACTCCTAGATGCTGC+TGG	-	33302:3189-3208	MS.gene53749:intergenic	50.0%
!	AGATTTGGCCTTCACCAGAG+GGG	+	33302:2800-2819	MS.gene53749:CDS	50.0%
!	GAAGGCCAAATCTCAGGTTG+AGG	-	33302:2793-2812	MS.gene53749:intergenic	50.0%
!	GAGATTTGGCCTTCACCAGA+GGG	+	33302:2799-2818	MS.gene53749:CDS	50.0%
!	GTGCAAGAGACAGTGCCATT+TGG	+	33302:3306-3325	MS.gene53749:CDS	50.0%
!	TGAGATTTGGCCTTCACCAG+AGG	+	33302:2798-2817	MS.gene53749:CDS	50.0%
!!	AGAGACAGTGCCATTTGGTG+AGG	+	33302:3311-3330	MS.gene53749:CDS	50.0%
!!	CCGCCATTTCAAGTTGGACT+TGG	-	33302:3146-3165	MS.gene53749:intergenic	50.0%
!!	TTGATGGACTCAATGGTGGC+TGG	+	33302:3279-3298	MS.gene53749:CDS	50.0%
	TCACTCCTAGATGCTGCTGG+AGG	-	33302:3186-3205	MS.gene53749:intergenic	55.0%
!	GATTTGGCCTTCACCAGAGG+GGG	+	33302:2801-2820	MS.gene53749:CDS	55.0%
	AGCACAGATTGGACCCCCTC+TGG	-	33302:2817-2836	MS.gene53749:intergenic	60.0%
	CACCACCTCCAGCAGCATCT+AGG	+	33302:3178-3197	MS.gene53749:CDS	60.0%
	CTAGATGCTGCTGGAGGTGG+TGG	-	33302:3180-3199	MS.gene53749:intergenic	60.0%
	CTCCTAGATGCTGCTGGAGG+TGG	-	33302:3183-3202	MS.gene53749:intergenic	60.0%
	GATTGGACCCCCTCTGGTGA+AGG	-	33302:2811-2830	MS.gene53749:intergenic	60.0%
!	GATGCTGCTGGAGGTGGTGG+CGG	-	33302:3177-3196	MS.gene53749:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
33302	gene	2709	3368	2709	ID=MS.gene53749
33302	mRNA	2709	3368	2709	ID=MS.gene53749.t1;Parent=MS.gene53749
33302	exon	2709	3368	2709	ID=MS.gene53749.t1.exon1;Parent=MS.gene53749.t1
33302	CDS	2709	3368	2709	ID=cds.MS.gene53749.t1;Parent=MS.gene53749.t1

Gene Sequence

>MS.gene53749

ATGGGTCTATTGAAAAAGGTTGATGAACTTAGCACCCTCTGTGGCATTGAAGCTTGTGCCATAGTGTATGGCCCTTATGAGCCTCAACCTGAGATTTGGCCTTCACCAGAGGGGGTCCAATCTGTGCTCTCAAAATTCATGACGATGCATGAGTTTCAAAAGTGCAAGAAAATGATGAACCAAGAGACTTTCTTGACACAAAGGGTTCTGAAGACTGAAGAGAAATTGATGAAACAAAGAAAAGATAATAGGGAGCAAGAGATGACAATACTCATGGCTCAATGTCTTAATGAAGGCAAAGTTGTGCACAACAATATGTCAACAATGGATATGAGTTATCTTGCTTGGTTGATCGACCACAATTTGAATCACGTTGGTAGACGTTTGGAAGCATGGGATAACAATGATCAAAATCAAATCATTGCTATTCAAAACCAAGTCCAACTTGAAATGGCGGCAACAATACCGCCACCACCTCCAGCAGCATCTAGGAGTGAAGAAATGGCAATTATGGGTCATGGCCATGTTGGGATACCTATTAATGATGATGACATTATGCAAAGTCAATTATTGATGGACTCAATGGTGGCTGGTAATGTGCAAGAGACAGTGCCATTTGGTGAGGTCAATTCTGAAGTTTGGCCTGATTTATTACCTTGA

Protein sequence

>MS.gene53749.t1

MGLLKKVDELSTLCGIEACAIVYGPYEPQPEIWPSPEGVQSVLSKFMTMHEFQKCKKMMNQETFLTQRVLKTEEKLMKQRKDNREQEMTILMAQCLNEGKVVHNNMSTMDMSYLAWLIDHNLNHVGRRLEAWDNNDQNQIIAIQNQVQLEMAATIPPPPPAASRSEEMAIMGHGHVGIPINDDDIMQSQLLMDSMVAGNVQETVPFGEVNSEVWPDLLP